https://www.bosterbio.com/catalog/product/view/id/1292972026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11420-1-sez6l2-primary-antibodies-wb-testing-1.jpgAnti-SEZ6L2 Antibody Picoband® Western blot analysis of SEZ6L2 using anti-SEZ6L2 antibody (A11420-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEZ6L2 antigen affinity purified polyclonal antibody (A11420-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SEZ6L2 at approximately 180 kDa. The expected band size for SEZ6L2 is at 98 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11420-1-sez6l2-primary-antibodies-ihc-testing-2.jpgAnti-SEZ6L2 Antibody Picoband® IHC analysis of SEZ6L2 using anti-SEZ6L2 antibody (A11420-1). SEZ6L2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEZ6L2 Antibody (A11420-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11420-1-sez6l2-primary-antibodies-ihc-testing-3.jpgAnti-SEZ6L2 Antibody Picoband® IHC analysis of SEZ6L2 using anti-SEZ6L2 antibody (A11420-1). SEZ6L2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEZ6L2 Antibody (A11420-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11420-1-sez6l2-primary-antibodies-ihc-testing-4.jpgAnti-SEZ6L2 Antibody Picoband® IHC analysis of SEZ6L2 using anti-SEZ6L2 antibody (A11420-1). SEZ6L2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEZ6L2 Antibody (A11420-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11420-1-sez6l2-primary-antibodies-fcm-testing-5.jpgAnti-SEZ6L2 Antibody Picoband® Flow Cytometry analysis of U251 cells using anti-SEZ6L2 antibody (A11420-1). Overlay histogram showing U251 cells stained with A11420-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SEZ6L2 Antibody (A11420-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11420-1-sez6l2-primary-antibodies-ihc-testing-5.jpgAnti-SEZ6L2 Antibody Picoband®IHC analysis of SEZ6L2 using anti-SEZ6L2 antibody (A11420-1). SEZ6L2 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEZ6L2 Antibody (A11420-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1292982026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14271-2-sgcz-primary-antibodies-wb-testing-1.jpgAnti-SGCZ Antibody Picoband® Western blot analysis of SGCZ using anti-SGCZ antibody (A14271-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGCZ antigen affinity purified polyclonal antibody (A14271-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SGCZ at approximately 33 kDa. The expected band size for SGCZ is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14271-2-sgcz-primary-antibodies-fcm-testing-2.jpgAnti-SGCZ Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SGCZ antibody (A14271-2). Overlay histogram showing HEL cells stained with A14271-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SGCZ Antibody (A14271-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1292992026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07564-1-smap2-primary-antibodies-wb-testing-1.jpgAnti-SMAP2 Antibody Picoband® Western blot analysis of SMAP2 using anti-SMAP2 antibody (A07564-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAP2 antigen affinity purified polyclonal antibody (A07564-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMAP2 at approximately 47 kDa. The expected band size for SMAP2 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07564-1-smap2-primary-antibodies-ip-testing-1.jpgAnti-SMAP2 Antibody Picoband®Immunoprecipitating (IP) SMAP2 in K562 whole cell lysate. Western blot analysis of SMAP2 using anti-SMAP2 antibody (A07564-1); Lane 1: K562 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-SMAP2 antibody in K562 whole cell lysate; Lane 3: anti-SMAP2 antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SMAP2 antigen affinity purified polyclonal antibody (A07564-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SMAP2 at approximately 47 kDa. The expected band size for SMAP2 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07564-1-smap2-primary-antibodies-fcm-testing-2.jpgAnti-SMAP2 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-SMAP2 antibody (A07564-1). Overlay histogram showing THP-1 cells stained with A07564-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMAP2 Antibody (A07564-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293002026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03613-1-spred1-primary-antibodies-wb-testing-1.jpgAnti-SPRED1 Antibody Picoband® Western blot analysis of SPRED1 using anti-SPRED1 antibody (A03613-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPRED1 antigen affinity purified polyclonal antibody (A03613-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPRED1 at approximately 50 kDa. The expected band size for SPRED1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03613-1-spred1-primary-antibodies-if-testing-2.jpgAnti-SPRED1 Antibody Picoband® IF analysis of SPRED1 using anti-SPRED1 antibody (A03613-1). SPRED1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SPRED1 Antibody (A03613-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03613-1-spred1-primary-antibodies-fcm-testing-3.jpgAnti-SPRED1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SPRED1 antibody (A03613-1). Overlay histogram showing HEL cells stained with A03613-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SPRED1 Antibody (A03613-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293012026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14169-1-spopl-primary-antibodies-wb-testing-1.jpgAnti-SPOPL Antibody Picoband® Western blot analysis of SPOPL using anti-SPOPL antibody (A14169-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPOPL antigen affinity purified polyclonal antibody (A14169-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPOPL at approximately 45 kDa. The expected band size for SPOPL is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14169-1-spopl-primary-antibodies-ihc-testing-2.jpgAnti-SPOPL Antibody Picoband® IHC analysis of SPOPL using anti-SPOPL antibody (A14169-1). SPOPL was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPOPL Antibody (A14169-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14169-1-spopl-primary-antibodies-ihc-testing-3.jpgAnti-SPOPL Antibody Picoband® IHC analysis of SPOPL using anti-SPOPL antibody (A14169-1). SPOPL was detected in a paraffin-embedded section of mouse bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPOPL Antibody (A14169-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14169-1-spopl-primary-antibodies-ihc-testing-4.jpgAnti-SPOPL Antibody Picoband® IHC analysis of SPOPL using anti-SPOPL antibody (A14169-1). SPOPL was detected in a paraffin-embedded section of mouse bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPOPL Antibody (A14169-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14169-1-spopl-primary-antibodies-if-testing-5.jpgAnti-SPOPL Antibody Picoband® IF analysis of SPOPL using anti-SPOPL antibody (A14169-1) and anti-Beta Tubulin antibody (M01857-3). SPOPL was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SPOPL Antibody (A14169-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14169-1-spopl-primary-antibodies-ip-testing-6.jpgAnti-SPOPL Antibody Picoband® Immunoprecipitating SPOPL in MCF-7 whole cell lysate. Western blot analysis of SPOPL using anti-SPOPL antibody (A14169-1). Lane 1: MCF-7 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-SPOPL antibody in MCF-7 whole cell lysate, Lane 3: anti-SPOPL antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SPOPL antigen affinity purified polyclonal antibody (A14169-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SPOPL at approximately 45 kDa. The expected band size for SPOPL is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14169-1-spopl-primary-antibodies-fcm-testing-7.jpgAnti-SPOPL Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-SPOPL antibody (A14169-1). Overlay histogram showing PC-3 cells stained with A14169-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPOPL Antibody (A14169-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293022026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31838-1-srarp-primary-antibodies-wb-testing-1.jpgAnti-SRARP Antibody Picoband® Western blot analysis of SRARP using anti-SRARP antibody (A31838-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRARP antigen affinity purified polyclonal antibody (A31838-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SRARP at approximately 18 kDa. The expected band size for SRARP is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31838-1-srarp-primary-antibodies-ihc-testing-2.jpgAnti-SRARP Antibody Picoband® IHC analysis of SRARP using anti-SRARP antibody (A31838-1). SRARP was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRARP Antibody (A31838-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31838-1-srarp-primary-antibodies-ihc-testing-3.jpgAnti-SRARP Antibody Picoband® IHC analysis of SRARP using anti-SRARP antibody (A31838-1). SRARP was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRARP Antibody (A31838-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1293032026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12736-1-srbd1-primary-antibodies-wb-testing-1.jpgAnti-SRBD1 Antibody Picoband® Western blot analysis of SRBD1 using anti-SRBD1 antibody (A12736-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRBD1 antigen affinity purified polyclonal antibody (A12736-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SRBD1 at approximately 70 kDa. The expected band size for SRBD1 is at 112 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12736-1-srbd1-primary-antibodies-ihc-testing-2.jpgAnti-SRBD1 Antibody Picoband® IHC analysis of SRBD1 using anti-SRBD1 antibody (A12736-1). SRBD1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRBD1 Antibody (A12736-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12736-1-srbd1-primary-antibodies-fcm-testing-3.jpgAnti-SRBD1 Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-SRBD1 antibody (A12736-1). Overlay histogram showing HEL cells stained with A12736-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRBD1 Antibody (A12736-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293042026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05964-1-stambp-primary-antibodies-wb-testing-1.jpgAnti-STAMBP Antibody Picoband® Western blot analysis of STAMBP using anti-STAMBP antibody (A05964-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey Cos-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAMBP antigen affinity purified polyclonal antibody (A05964-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STAMBP at approximately 50 kDa. The expected band size for STAMBP is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05964-1-stambp-primary-antibodies-ihc-testing-2.jpgAnti-STAMBP Antibody Picoband® IHC analysis of STAMBP using anti-STAMBP antibody (A05964-1). STAMBP was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAMBP Antibody (A05964-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05964-1-stambp-primary-antibodies-ihc-testing-3.jpgAnti-STAMBP Antibody Picoband® IHC analysis of STAMBP using anti-STAMBP antibody (A05964-1). STAMBP was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAMBP Antibody (A05964-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05964-1-stambp-primary-antibodies-ihc-testing-4.jpgAnti-STAMBP Antibody Picoband® IHC analysis of STAMBP using anti-STAMBP antibody (A05964-1). STAMBP was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAMBP Antibody (A05964-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05964-1-stambp-primary-antibodies-ihc-testing-5.jpgAnti-STAMBP Antibody Picoband® IHC analysis of STAMBP using anti-STAMBP antibody (A05964-1). STAMBP was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAMBP Antibody (A05964-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05964-1-stambp-primary-antibodies-ihc-testing-6.jpgAnti-STAMBP Antibody Picoband® IHC analysis of STAMBP using anti-STAMBP antibody (A05964-1). STAMBP was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAMBP Antibody (A05964-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05964-1-stambp-primary-antibodies-ihc-testing-7.jpgAnti-STAMBP Antibody Picoband® IHC analysis of STAMBP using anti-STAMBP antibody (A05964-1). STAMBP was detected in a paraffin-embedded section of human rectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAMBP Antibody (A05964-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05964-1-stambp-primary-antibodies-ihc-testing-8.jpgAnti-STAMBP Antibody Picoband® IHC analysis of STAMBP using anti-STAMBP antibody (A05964-1). STAMBP was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAMBP Antibody (A05964-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05964-1-stambp-primary-antibodies-if-testing-9.jpgAnti-STAMBP Antibody Picoband® IF analysis of STAMBP using anti-STAMBP antibody (A05964-1). STAMBP was detected in an immunocytochemical section of PC3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STAMBP Antibody (A05964-1) overnight at 4°C. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05964-1-stambp-primary-antibodies-fcm-testing-10.jpgAnti-STAMBP Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-STAMBP antibody (A05964-1). Overlay histogram showing Jurkat cells stained with A05964-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAMBP Antibody (A05964-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293052026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08436-1-stk17a-primary-antibodies-wb-testing-1.jpgAnti-STK17A Antibody Picoband® Western blot analysis of STK17A using anti-STK17A antibody (A08436-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STK17A antigen affinity purified polyclonal antibody (A08436-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STK17A at approximately 53 kDa. The expected band size for STK17A is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08436-1-stk17a-primary-antibodies-ip-testing-2.jpgAnti-STK17A Antibody Picoband® Immunoprecipitating STK17A in U251 whole cell lysate. Western blot analysis of STK17A using anti-STK17A antibody (A08436-1). Lane 1: U251 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-STK17A antibody in U251 whole cell lysate, Lane 3: anti-STK17A antibody (2μg) + U251 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-STK17A antigen affinity purified polyclonal antibody (A08436-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for STK17A at approximately 53 kDa. The expected band size for STK17A is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08436-1-stk17a-primary-antibodies-fcm-testing-3.jpgAnti-STK17A Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-STK17A antibody (A08436-1). Overlay histogram showing PC-3 cells stained with A08436-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STK17A Antibody (A08436-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293062026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03160-1-ubr5-primary-antibodies-wb-testing-1.jpgAnti-UBR5 Antibody Picoband® Western blot analysis of UBR5 using anti-UBR5 antibody (A03160-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBR5 antigen affinity purified polyclonal antibody (A03160-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBR5 at approximately 280 kDa. The expected band size for UBR5 is at 309 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03160-1-ubr5-primary-antibodies-fcm-testing-2.jpgAnti-UBR5 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-UBR5 antibody (A03160-1). Overlay histogram showing MCF-7 cells stained with A03160-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBR5 Antibody (A03160-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293072026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02658-2-rad51b-primary-antibodies-wb-testing-1.jpgAnti-RAD51L1/RAD51B Antibody Picoband® Western blot analysis of RAD51L1/RAD51B using anti-RAD51L1/RAD51B antibody (A02658-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51L1/RAD51B antigen affinity purified polyclonal antibody (A02658-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAD51L1/RAD51B at approximately 42 kDa. The expected band size for RAD51L1/RAD51B is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02658-2-rad51b-primary-antibodies-if-testing-2.jpgAnti-RAD51L1/RAD51B Antibody Picoband® IF analysis of RAD51L1/RAD51B using anti-RAD51L1/RAD51B antibody (A02658-2) and anti-Beta Tubulin antibody (M01857-3). RAD51L1/RAD51B was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAD51L1/RAD51B Antibody (A02658-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02658-2-rad51b-primary-antibodies-fcm-testing-3.jpgAnti-RAD51L1/RAD51B Antibody Picoband® Flow Cytometry analysis of U87 cells using anti-RAD51L1/RAD51B antibody (A02658-2). Overlay histogram showing U87 cells stained with A02658-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAD51L1/RAD51B Antibody (A02658-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293082026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14717-1-ralgapb-primary-antibodies-wb-testing-1.jpgAnti-RALGAPB Antibody Picoband® Western blot analysis of RALGAPB using anti-RALGAPB antibody (A14717-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALGAPB antigen affinity purified polyclonal antibody (A14717-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RALGAPB at approximately 170 kDa. The expected band size for RALGAPB is at 167 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14717-1-ralgapb-primary-antibodies-ihc-testing-2.jpgAnti-RALGAPB Antibody Picoband® IHC analysis of RALGAPB using anti-RALGAPB antibody (A14717-1). RALGAPB was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RALGAPB Antibody (A14717-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14717-1-ralgapb-primary-antibodies-ihc-testing-3.jpgAnti-RALGAPB Antibody Picoband® IHC analysis of RALGAPB using anti-RALGAPB antibody (A14717-1). RALGAPB was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RALGAPB Antibody (A14717-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14717-1-ralgapb-primary-antibodies-ihc-testing-4.jpgAnti-RALGAPB Antibody Picoband® IHC analysis of RALGAPB using anti-RALGAPB antibody (A14717-1). RALGAPB was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RALGAPB Antibody (A14717-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1293092026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06130-1-raph1-primary-antibodies-wb-testing-1.jpgAnti-RAPH1 Antibody Picoband® Western blot analysis of RAPH1 using anti-RAPH1 antibody (A06130-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAPH1 antigen affinity purified polyclonal antibody (A06130-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAPH1 at approximately 195 kDa. The expected band size for RAPH1 is at 135 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06130-1-raph1-primary-antibodies-ihc-testing-2.jpgAnti-RAPH1 Antibody Picoband® IHC analysis of RAPH1 using anti-RAPH1 antibody (A06130-1). RAPH1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAPH1 Antibody (A06130-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06130-1-raph1-primary-antibodies-ihc-testing-3.jpgAnti-RAPH1 Antibody Picoband® IHC analysis of RAPH1 using anti-RAPH1 antibody (A06130-1). RAPH1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAPH1 Antibody (A06130-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1293102026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10485-1-rars2-primary-antibodies-wb-testing-1.jpgAnti-RARS2 Antibody Picoband® Western blot analysis of RARS2 using anti-RARS2 antibody (A10485-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RARS2 antigen affinity purified polyclonal antibody (A10485-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RARS2 at approximately 66 kDa. The expected band size for RARS2 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10485-1-rars2-primary-antibodies-fcm-testing-2.jpgAnti-RARS2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-RARS2 antibody (A10485-1). Overlay histogram showing MCF-7 cells stained with A10485-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RARS2 Antibody (A10485-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293112026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11713-1-rasal3-primary-antibodies-wb-testing-1.jpgAnti-RASAL3 Antibody Picoband® Western blot analysis of RASAL3 using anti-RASAL3 antibody (A11713-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASAL3 antigen affinity purified polyclonal antibody (A11713-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RASAL3 at approximately 104 kDa. The expected band size for RASAL3 is at 112 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11713-1-rasal3-primary-antibodies-ihc-testing-2.jpgAnti-RASAL3 Antibody Picoband® IHC analysis of RASAL3 using anti-RASAL3 antibody (A11713-1). RASAL3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RASAL3 Antibody (A11713-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11713-1-rasal3-primary-antibodies-fcm-testing-3.jpgAnti-RASAL3 Antibody Picoband® Flow Cytometry analysis of Raji cells using anti-RASAL3 antibody (A11713-1). Overlay histogram showing Raji cells stained with A11713-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RASAL3 Antibody (A11713-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293122026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11612-2-rps19bp1-primary-antibodies-wb-testing-1.jpgAnti-RPS19BP1 Antibody Picoband® Western blot analysis of RPS19BP1 using anti-RPS19BP1 antibody (A11612-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS19BP1 antigen affinity purified polyclonal antibody (A11612-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RPS19BP1 at approximately 17 kDa. The expected band size for RPS19BP1 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11612-2-rps19bp1-primary-antibodies-if-testing-2.jpgAnti-RPS19BP1 Antibody Picoband® IF analysis of RPS19BP1 using anti-RPS19BP1 antibody (A11612-2) and anti-Beta Tubulin antibody (M01857-3). RPS19BP1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPS19BP1 Antibody (A11612-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11612-2-rps19bp1-primary-antibodies-fcm-testing-3.jpgAnti-RPS19BP1 Antibody Picoband® Flow Cytometry analysis of CACO-2 cells using anti-RPS19BP1 antibody (A11612-2). Overlay histogram showing CACO-2 cells stained with A11612-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS19BP1 Antibody (A11612-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293132026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07477-1-osbpl5-primary-antibodies-wb-testing-1.jpgAnti-OSBPL5 Antibody Picoband® Western blot analysis of OSBPL5 using anti-OSBPL5 antibody (A07477-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSBPL5 antigen affinity purified polyclonal antibody (A07477-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OSBPL5 at approximately 120 kDa. The expected band size for OSBPL5 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07477-1-osbpl5-primary-antibodies-if-testing-2.jpgAnti-OSBPL5 Antibody Picoband® IF analysis of OSBPL5 using anti-OSBPL5 antibody (A07477-1). OSBPL5 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OSBPL5 Antibody (A07477-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07477-1-osbpl5-primary-antibodies-fcm-testing-3.jpgAnti-OSBPL5 Antibody Picoband® Flow Cytometry analysis of MOLT-4 cells using anti-OSBPL5 antibody (A07477-1). Overlay histogram showing MOLT-4 cells stained with A07477-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OSBPL5 Antibody (A07477-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293142026-03-17T05:16:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02961-3-oscar-primary-antibodies-wb-testing-1.jpgAnti-OSCAR Antibody Picoband® Western blot analysis of OSCAR using anti-OSCAR antibody (A02961-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSCAR antigen affinity purified polyclonal antibody (A02961-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OSCAR at approximately 30 kDa. The expected band size for OSCAR is at 30 kDa. https://www.bosterbio.com/catalog/product/view/id/1293152026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02228-2-pde4b-primary-antibodies-wb-testing-1.jpgAnti-PDE4B Antibody Picoband® Western blot analysis of PDE4B using anti-PDE4B antibody (A02228-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE4B antigen affinity purified polyclonal antibody (A02228-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDE4B at approximately 90 kDa. The expected band size for PDE4B is at 83 kDa. https://www.bosterbio.com/catalog/product/view/id/1293162026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11752-1-pfdn2-primary-antibodies-wb-testing-1.jpgAnti-PFDN2 Antibody Picoband® Western blot analysis of PFDN2 using anti-PFDN2 antibody (A11752-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cel lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFDN2 antigen affinity purified polyclonal antibody (A11752-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PFDN2 at approximately 17 kDa. The expected band size for PFDN2 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11752-1-pfdn2-primary-antibodies-if-testing-2.jpgAnti-PFDN2 Antibody Picoband® IF analysis of PFDN2 using anti-PFDN2 antibody (A11752-1) and anti-Beta Tubulin antibody (M01857-3). PFDN2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PFDN2 Antibody (A11752-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11752-1-pfdn2-primary-antibodies-fcm-testing-3.jpgAnti-PFDN2 Antibody Picoband® Flow Cytometry analysis of PC-3 cells using anti-PFDN2 antibody (A11752-1). Overlay histogram showing PC-3 cells stained with A11752-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFDN2 Antibody (A11752-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293172026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12112-1-pitpnc1-primary-antibodies-wb-testing-1.jpgAnti-PITPNC1 Antibody Picoband® Western blot analysis of PITPNC1 using anti-PITPNC1 antibody (A12112-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITPNC1 antigen affinity purified polyclonal antibody (A12112-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PITPNC1 at approximately 38 kDa. The expected band size for PITPNC1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12112-1-pitpnc1-primary-antibodies-fcm-testing-2.jpgAnti-PITPNC1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PITPNC1 antibody (A12112-1). Overlay histogram showing MCF-7 cells stained with A12112-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PITPNC1 Antibody (A12112-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293182026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11077-2-polr1e-primary-antibodies-wb-testing-1.jpgAnti-POLR1E Antibody Picoband® Western blot analysis of POLR1E using anti-POLR1E antibody (A11077-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR1E antigen affinity purified polyclonal antibody (A11077-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POLR1E at approximately 47 kDa. The expected band size for POLR1E is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11077-2-polr1e-primary-antibodies-if-testing-2.jpgAnti-POLR1E Antibody Picoband® IF analysis of POLR1E using anti-POLR1E antibody (A11077-2) and anti-Beta Tubulin antibody (M01857-3). POLR1E was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-POLR1E Antibody (A11077-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11077-2-polr1e-primary-antibodies-ip-testing-3.jpgAnti-POLR1E Antibody Picoband® Immunoprecipitating POLR1E in RT4 whole cell lysate. Western blot analysis of POLR1E using anti-POLR1E antibody (A11077-2). Lane 1: RT4 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-POLR1E antibody in RT4 whole cell lysate, Lane 3: anti-POLR1E antibody (2μg) + RT4 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POLR1E antigen affinity purified polyclonal antibody (A11077-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for POLR1E at approximately 47 kDa. The expected band size for POLR1E is at 47 kDa. https://www.bosterbio.com/catalog/product/view/id/1293192026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08778-1-pold4-primary-antibodies-wb-testing-1.jpgAnti-POLD4 Antibody Picoband® Western blot analysis of POLD4 using anti-POLD4 antibody (A08778-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLD4 antigen affinity purified polyclonal antibody (A08778-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POLD4 at approximately 16 kDa. The expected band size for POLD4 is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08778-1-pold4-primary-antibodies-if-testing-2.jpgAnti-POLD4 Antibody Picoband® IF analysis of POLD4 using anti-POLD4 antibody (A08778-1) and anti-Beta Tubulin antibody (M01857-3). POLD4 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-POLD4 Antibody (A08778-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08778-1-pold4-primary-antibodies-fcm-testing-3.jpgAnti-POLD4 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-POLD4 antibody (A08778-1). Overlay histogram showing THP-1 cells stained with A08778-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POLD4 Antibody (A08778-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293202026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10026-1-ppih-primary-antibodies-wb-testing-1.jpgAnti-PPIH Antibody Picoband® Western blot analysis of PPIH using anti-PPIH antibody (A10026-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPIH antigen affinity purified polyclonal antibody (A10026-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPIH at approximately 19 kDa. The expected band size for PPIH is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10026-1-ppih-primary-antibodies-if-testing-2.jpgAnti-PPIH Antibody Picoband® IF analysis of PPIH using anti-PPIH antibody (A10026-1) and anti-Beta Tubulin antibody (M01857-3). PPIH was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPIH Antibody (A10026-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1293212026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07588-2-ppp2r5e-primary-antibodies-wb-testing-1.jpgAnti-PPP2R5E Antibody Picoband® Western blot analysis of PPP2R5E using anti-PPP2R5E antibody (A07588-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R5E antigen affinity purified polyclonal antibody (A07588-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPP2R5E at approximately 51 kDa. The expected band size for PPP2R5E is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07588-2-ppp2r5e-primary-antibodies-ihc-testing-2.jpgAnti-PPP2R5E Antibody Picoband® IHC analysis of PPP2R5E using anti-PPP2R5E antibody (A07588-2). PPP2R5E was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2R5E Antibody (A07588-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07588-2-ppp2r5e-primary-antibodies-ihc-testing-3.jpgAnti-PPP2R5E Antibody Picoband® IHC analysis of PPP2R5E using anti-PPP2R5E antibody (A07588-2). PPP2R5E was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2R5E Antibody (A07588-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07588-2-ppp2r5e-primary-antibodies-ihc-testing-4.jpgAnti-PPP2R5E Antibody Picoband® IHC analysis of PPP2R5E using anti-PPP2R5E antibody (A07588-2). PPP2R5E was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2R5E Antibody (A07588-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07588-2-ppp2r5e-primary-antibodies-fcm-testing-5.jpgAnti-PPP2R5E Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-PPP2R5E antibody (A07588-2). Overlay histogram showing A431 cells stained with A07588-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2R5E Antibody (A07588-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07588-2-ppp2r5e-primary-antibodies-fcm-testing-6.jpgAnti-PPP2R5E Antibody Picoband® Flow Cytometry analysis of U20S cells using anti-PPP2R5E antibody (A07588-2). Overlay histogram showing U20S cells stained with A07588-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2R5E Antibody (A07588-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293222026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04710-1-psma6-primary-antibodies-wb-testing-1.jpgAnti-PSMA6 Antibody Picoband® Western blot analysis of PSMA6 using anti-PSMA6 antibody (A04710-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA6 antigen affinity purified polyclonal antibody (A04710-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMA6 at approximately 27 kDa. The expected band size for PSMA6 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04710-1-psma6-primary-antibodies-if-testing-2.jpgAnti-PSMA6 Antibody Picoband® IF analysis of PSMA6 using anti-PSMA6 antibody (A04710-1). PSMA6 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMA6 Antibody (A04710-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1293232026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10795-1-ptcd3-primary-antibodies-wb-testing-1.jpgAnti-PTCD3 Antibody Picoband® Western blot analysis of PTCD3 using anti-PTCD3 antibody (A10795-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTCD3 antigen affinity purified polyclonal antibody (A10795-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTCD3 at approximately 79 kDa. The expected band size for PTCD3 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10795-1-ptcd3-primary-antibodies-ihc-testing-2.jpgAnti-PTCD3 Antibody Picoband® IHC analysis of PTCD3 using anti-PTCD3 antibody (A10795-1). PTCD3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTCD3 Antibody (A10795-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10795-1-ptcd3-primary-antibodies-ihc-testing-3.jpgAnti-PTCD3 Antibody Picoband® IHC analysis of PTCD3 using anti-PTCD3 antibody (A10795-1). PTCD3 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTCD3 Antibody (A10795-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10795-1-ptcd3-primary-antibodies-ihc-testing-4.jpgAnti-PTCD3 Antibody Picoband® IHC analysis of PTCD3 using anti-PTCD3 antibody (A10795-1). PTCD3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTCD3 Antibody (A10795-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10795-1-ptcd3-primary-antibodies-ihc-testing-5.jpgAnti-PTCD3 Antibody Picoband® IHC analysis of PTCD3 using anti-PTCD3 antibody (A10795-1). PTCD3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTCD3 Antibody (A10795-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10795-1-ptcd3-primary-antibodies-fcm-testing-6.jpgAnti-PTCD3 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-PTCD3 antibody (A10795-1). Overlay histogram showing A549 cells stained with A10795-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTCD3 Antibody (A10795-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293242026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05574-1-ptpre-primary-antibodies-wb-testing-1.jpgAnti-PTPRE Antibody Picoband® Western blot analysis of PTPRE using anti-PTPRE antibody (A05574-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPRE antigen affinity purified polyclonal antibody (A05574-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTPRE at approximately 81 kDa. The expected band size for PTPRE is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05574-1-ptpre-primary-antibodies-ihc-testing-2.jpgAnti-PTPRE Antibody Picoband® IHC analysis of PTPRE using anti-PTPRE antibody (A05574-1). PTPRE was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTPRE Antibody (A05574-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05574-1-ptpre-primary-antibodies-ihc-testing-3.jpgAnti-PTPRE Antibody Picoband® IHC analysis of PTPRE using anti-PTPRE antibody (A05574-1). PTPRE was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTPRE Antibody (A05574-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05574-1-ptpre-primary-antibodies-ihc-testing-4.jpgAnti-PTPRE Antibody Picoband® IHC analysis of PTPRE using anti-PTPRE antibody (A05574-1). PTPRE was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTPRE Antibody (A05574-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05574-1-ptpre-primary-antibodies-ihc-testing-5.jpgAnti-PTPRE Antibody Picoband® IHC analysis of PTPRE using anti-PTPRE antibody (A05574-1). PTPRE was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTPRE Antibody (A05574-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05574-1-ptpre-primary-antibodies-ihc-testing-6.jpgAnti-PTPRE Antibody Picoband® IHC analysis of PTPRE using anti-PTPRE antibody (A05574-1). PTPRE was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTPRE Antibody (A05574-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05574-1-ptpre-primary-antibodies-if-testing-7.jpgAnti-PTPRE Antibody Picoband® IF analysis of PTPRE using anti-PTPRE antibody (A05574-1). PTPRE was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PTPRE Antibody (A05574-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05574-1-ptpre-primary-antibodies-fcm-testing-8.jpgAnti-PTPRE Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-PTPRE antibody (A05574-1). Overlay histogram showing Jurkat cells stained with A05574-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PTPRE Antibody (A05574-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293252026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03116-1-ptprn-primary-antibodies-wb-testing-1.jpgAnti-IA-2/PTPRN Antibody Picoband® Western blot analysis of IA-2/PTPRN using anti-IA-2/PTPRN antibody (A03116-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IA-2/PTPRN antigen affinity purified polyclonal antibody (A03116-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IA-2/PTPRN at approximately 106 kDa. The expected band size for IA-2/PTPRN is at 106 kDa. https://www.bosterbio.com/catalog/product/view/id/1293262026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12115-1-naa30-primary-antibodies-wb-testing-1.jpgAnti-NAT12/NAA30 Antibody Picoband® Western blot analysis of NAT12/NAA30 using anti-NAT12/NAA30 antibody (A12115-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAT12/NAA30 antigen affinity purified polyclonal antibody (A12115-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NAT12/NAA30 at approximately 39 kDa. The expected band size for NAT12/NAA30 is at 39 kDa. https://www.bosterbio.com/catalog/product/view/id/1293272026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05057-1-nagk-primary-antibodies-wb-testing-1.jpgAnti-NAGK Antibody Picoband® Western blot analysis of NAGK using anti-NAGK antibody (A05057-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A431 whole cell lysates. Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAGK antigen affinity purified polyclonal antibody (A05057-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NAGK at approximately 37 kDa. The expected band size for NAGK is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05057-1-nagk-primary-antibodies-ihc-testing-2.jpgAnti-NAGK Antibody Picoband® IHC analysis of NAGK using anti-NAGK antibody (A05057-1). NAGK was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NAGK Antibody (A05057-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05057-1-nagk-primary-antibodies-ihc-testing-3.jpgAnti-NAGK Antibody Picoband® IHC analysis of NAGK using anti-NAGK antibody (A05057-1). NAGK was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NAGK Antibody (A05057-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05057-1-nagk-primary-antibodies-fcm-testing-4.jpgAnti-NAGK Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-NAGK antibody (A05057-1). Overlay histogram showing A431 cells stained with A05057-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NAGK Antibody (A05057-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293282026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04996-1-nek7-primary-antibodies-wb-testing-1.jpgAnti-NEK7 Antibody Picoband® Western blot analysis of NEK7 using anti-NEK7 antibody (A04996-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEK7 antigen affinity purified polyclonal antibody (A04996-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NEK7 at approximately 32 kDa. The expected band size for NEK7 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04996-1-nek7-primary-antibodies-ihc-testing-1.jpgAnti-NEK7 Antibody Picoband®IHC analysis of NEK7 using anti-NEK7 antibody (A04996-1). NEK7 was detected in a paraffin-embedded section of mouse bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEK7 Antibody (A04996-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04996-1-nek7-primary-antibodies-ihc-testing-2.jpgAnti-NEK7 Antibody Picoband®IHC analysis of NEK7 using anti-NEK7 antibody (A04996-1). NEK7 was detected in a paraffin-embedded section of rat bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEK7 Antibody (A04996-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04996-1-nek7-primary-antibodies-if-testing-2.jpgAnti-NEK7 Antibody Picoband® IF analysis of NEK7 using anti-NEK7 antibody (A04996-1) and anti-Beta Tubulin antibody (M01857-3). NEK7 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NEK7 Antibody (A04996-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04996-1-nek7-primary-antibodies-ip-testing-3.jpgAnti-NEK7 Antibody Picoband® Immunoprecipitating NEK7 in Jurkat whole cell lysate. Western blot analysis of NEK7 using anti-NEK7 antibody (A04996-1). Lane 1: Jurkat whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-NEK7 antibody in Jurkat whole cell lysate, Lane 3: anti-NEK7 antibody (2μg) + Jurkat whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NEK7 antigen affinity purified polyclonal antibody (A04996-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NEK7 at approximately 32 kDa. The expected band size for NEK7 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04996-1-nek7-primary-antibodies-fcm-testing-4.jpgAnti-NEK7 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-NEK7 antibody (A04996-1). Overlay histogram showing 293T cells stained with A04996-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEK7 Antibody (A04996-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293292026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03554-1-neurog3-primary-antibodies-wb-testing-1.jpgAnti-NEUROG3 Antibody Picoband® Western blot analysis of NEUROG3 using anti-NEUROG3 antibody (A03554-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEUROG3 antigen affinity purified polyclonal antibody (A03554-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NEUROG3 at approximately 27 kDa. The expected band size for NEUROG3 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03554-1-neurog3-primary-antibodies-fcm-testing-2.jpgAnti-NEUROG3 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-NEUROG3 antibody (A03554-1). Overlay histogram showing MCF-7 cells stained with A03554-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEUROG3 Antibody (A03554-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03554-1-neurog3-primary-antibodies-fcm-testing-3.jpgAnti-NEUROG3 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-NEUROG3 antibody (A03554-1). Overlay histogram showing THP-1 cells stained with A03554-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEUROG3 Antibody (A03554-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293302026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16309-1-nudt8-primary-antibodies-wb-testing-1.jpgAnti-NUDT8 Antibody Picoband® Western blot analysis of NUDT8 using anti-NUDT8 antibody (A16309-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT8 antigen affinity purified polyclonal antibody (A16309-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NUDT8 at approximately 25 kDa. The expected band size for NUDT8 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16309-1-nudt8-primary-antibodies-ihc-testing-2.jpgAnti-NUDT8 Antibody Picoband® IHC analysis of NUDT8 using anti-NUDT8 antibody (A16309-1). NUDT8 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUDT8 Antibody (A16309-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16309-1-nudt8-primary-antibodies-ihc-testing-3.jpgAnti-NUDT8 Antibody Picoband® IHC analysis of NUDT8 using anti-NUDT8 antibody (A16309-1). NUDT8 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUDT8 Antibody (A16309-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16309-1-nudt8-primary-antibodies-ihc-testing-4.jpgAnti-NUDT8 Antibody Picoband® IHC analysis of NUDT8 using anti-NUDT8 antibody (A16309-1). NUDT8 was detected in a paraffin-embedded section of human prostatic hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUDT8 Antibody (A16309-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16309-1-nudt8-primary-antibodies-fcm-testing-5.jpgAnti-NUDT8 Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-NUDT8 antibody (A16309-1). Overlay histogram showing A431 cells stained with A16309-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUDT8 Antibody (A16309-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293312026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10184-1-mplkip-primary-antibodies-wb-testing-1.jpgAnti-MPLKIP Antibody Picoband® Western blot analysis of MPLKIP using anti-MPLKIP antibody (A10184-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPLKIP antigen affinity purified polyclonal antibody (A10184-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MPLKIP at approximately 19 kDa. The expected band size for MPLKIP is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10184-1-mplkip-primary-antibodies-if-testing-2.jpgAnti-MPLKIP Antibody Picoband® IF analysis of MPLKIP using anti-MPLKIP antibody (A10184-1) and anti-Beta Tubulin antibody (M01857-3). MPLKIP was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MPLKIP Antibody (A10184-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1293322026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15162-1-lrguk-primary-antibodies-wb-testing-1.jpgAnti-LRGUK Antibody Picoband® Western blot analysis of LRGUK using anti-LRGUK antibody (A15162-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRGUK antigen affinity purified polyclonal antibody (A15162-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LRGUK at approximately 94 kDa. The expected band size for LRGUK is at 94 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15162-1-lrguk-primary-antibodies-fcm-testing-2.jpgAnti-LRGUK Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-LRGUK antibody (A15162-1). Overlay histogram showing SH-SY5Y cells stained with A15162-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LRGUK Antibody (A15162-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293332026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09440-1-iqsec2-primary-antibodies-wb-testing-1.jpgAnti-IQSEC2 Antibody Picoband® Western blot analysis of IQSEC2 using anti-IQSEC2 antibody (A09440-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IQSEC2 antigen affinity purified polyclonal antibody (A09440-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IQSEC2 at approximately 200 kDa. The expected band size for IQSEC2 is at 163 kDa. https://www.bosterbio.com/catalog/product/view/id/1293342026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10942-2-ivns1abp-primary-antibodies-wb-testing-1.jpgAnti-IVNS1ABP Antibody Picoband® Western blot analysis of IVNS1ABP using anti-IVNS1ABP antibody (A10942-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IVNS1ABP antigen affinity purified polyclonal antibody (A10942-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IVNS1ABP at approximately 72 kDa. The expected band size for IVNS1ABP is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10942-2-ivns1abp-primary-antibodies-ihc-testing-2.jpgAnti-IVNS1ABP Antibody Picoband® IHC analysis of IVNS1ABP using anti-IVNS1ABP antibody (A10942-2). IVNS1ABP was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IVNS1ABP Antibody (A10942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10942-2-ivns1abp-primary-antibodies-ihc-testing-3.jpgAnti-IVNS1ABP Antibody Picoband® IHC analysis of IVNS1ABP using anti-IVNS1ABP antibody (A10942-2). IVNS1ABP was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IVNS1ABP Antibody (A10942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10942-2-ivns1abp-primary-antibodies-ihc-testing-4.jpgAnti-IVNS1ABP Antibody Picoband® IHC analysis of IVNS1ABP using anti-IVNS1ABP antibody (A10942-2). IVNS1ABP was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IVNS1ABP Antibody (A10942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10942-2-ivns1abp-primary-antibodies-ihc-testing-5.jpgAnti-IVNS1ABP Antibody Picoband® IHC analysis of IVNS1ABP using anti-IVNS1ABP antibody (A10942-2). IVNS1ABP was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IVNS1ABP Antibody (A10942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10942-2-ivns1abp-primary-antibodies-ihc-testing-6.jpgAnti-IVNS1ABP Antibody Picoband® IHC analysis of IVNS1ABP using anti-IVNS1ABP antibody (A10942-2). IVNS1ABP was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IVNS1ABP Antibody (A10942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10942-2-ivns1abp-primary-antibodies-ihc-testing-7.jpgAnti-IVNS1ABP Antibody Picoband® IHC analysis of IVNS1ABP using anti-IVNS1ABP antibody (A10942-2). IVNS1ABP was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IVNS1ABP Antibody (A10942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10942-2-ivns1abp-primary-antibodies-ihc-testing-8.jpgAnti-IVNS1ABP Antibody Picoband® IHC analysis of IVNS1ABP using anti-IVNS1ABP antibody (A10942-2). IVNS1ABP was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IVNS1ABP Antibody (A10942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10942-2-ivns1abp-primary-antibodies-if-testing-9.jpgAnti-IVNS1ABP Antibody Picoband® IF analysis of IVNS1ABP using anti-IVNS1ABP antibody (A10942-2). IVNS1ABP was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-IVNS1ABP Antibody (A10942-2) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10942-2-ivns1abp-primary-antibodies-if-testing-10.jpgAnti-IVNS1ABP Antibody Picoband® IF analysis of IVNS1ABP using anti-IVNS1ABP antibody (A10942-2). IVNS1ABP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-IVNS1ABP Antibody (A10942-2) overnight at 4°C. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10942-2-ivns1abp-primary-antibodies-fcm-testing-11.jpgAnti-IVNS1ABP Antibody Picoband® Flow Cytometry analysis of HeLa cells using anti-IVNS1ABP antibody (A10942-2). Overlay histogram showing HeLa cells stained with A10942-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IVNS1ABP Antibody (A10942-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293352026-03-17T05:16:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31862-2-haspin-primary-antibodies-wb-testing-1.jpgAnti-GSG2/HASPIN Antibody Picoband® Western blot analysis of GSG2/HASPIN using anti-GSG2/HASPIN antibody (A31862-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSG2/HASPIN antigen affinity purified polyclonal antibody (A31862-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSG2/HASPIN at approximately 88 kDa. The expected band size for GSG2/HASPIN is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31862-2-haspin-primary-antibodies-if-testing-2.jpgAnti-GSG2/HASPIN Antibody Picoband® IF analysis of GSG2/HASPIN using anti-GSG2/HASPIN antibody (A31862-2). GSG2/HASPIN was detected in an immunocytochemical section of MG63 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GSG2/HASPIN Antibody (A31862-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31862-2-haspin-primary-antibodies-fcm-testing-3.jpgAnti-GSG2/HASPIN Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-GSG2/HASPIN antibody (A31862-2). Overlay histogram showing K562 cells stained with A31862-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSG2/HASPIN Antibody (A31862-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293362026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12994-2-haus8-primary-antibodies-wb-testing-1_1.jpgAnti-HAUS8 Antibody Picoband®Western blot analysis of HAUS8 using anti-HAUS8 antibody (A12994-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAUS8 antigen affinity purified polyclonal antibody (A12994-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HAUS8 at approximately 45 kDa. The expected band size for HAUS8 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12994-2-haus8-primary-antibodies-if-testing-1.jpgAnti-HAUS8 Antibody Picoband®IF analysis of HAUS8 using anti-HAUS8 antibody (A12994-2). HAUS8 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HAUS8 Antibody (A12994-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12994-2-haus8-primary-antibodies-ip-testing-1.jpgAnti-HAUS8 Antibody Picoband®Immunoprecipitating (IP) HAUS8 in MCF-7 whole cell lysate. Western blot analysis of HAUS8 using anti-HAUS8 antibody (A12994-2); Lane 1: MCF-7 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-HAUS8 antibody in MCF-7 whole cell lysate; Lane 3: anti-HAUS8 antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HAUS8 antigen affinity purified polyclonal antibody (A12994-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for HAUS8 at approximately 42 kDa. The expected band size for HAUS8 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12994-2-haus8-primary-antibodies-fcm-testing-1.jpgAnti-HAUS8 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-HAUS8 antibody (A12994-2). Overlay histogram showing RT4 cells stained with A12994-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HAUS8 Antibody (A12994-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293372026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12411-1-ghdc-primary-antibodies-wb-testing-1.jpgAnti-GHDC Antibody Picoband® Western blot analysis of GHDC using anti-GHDC antibody (A12411-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHDC antigen affinity purified polyclonal antibody (A12411-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GHDC at approximately 54 kDa. The expected band size for GHDC is at 54 kDa. https://www.bosterbio.com/catalog/product/view/id/1293382026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01860-2-epha4-primary-antibodies-wb-testing-1.jpgAnti-EPHA4 Antibody Picoband® Western blot analysis of EPHA4 using anti-EPHA4 antibody (A01860-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHA4 antigen affinity purified polyclonal antibody (A01860-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPHA4 at approximately 135 kDa. The expected band size for EPHA4 is at 110 kDa. https://www.bosterbio.com/catalog/product/view/id/1293392026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03126-2-fubp1-primary-antibodies-wb-testing-1.jpgAnti-FUBP1 Antibody Picoband® Western blot analysis of FUBP1 using anti-FUBP1 antibody (A03126-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FUBP1 antigen affinity purified polyclonal antibody (A03126-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FUBP1 at approximately 74 kDa. The expected band size for FUBP1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03126-2-fubp1-primary-antibodies-ihc-testing-2.jpgAnti-FUBP1 Antibody Picoband® IHC analysis of FUBP1 using anti-FUBP1 antibody (A03126-2). FUBP1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FUBP1 Antibody (A03126-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03126-2-fubp1-primary-antibodies-ihc-testing-3.jpgAnti-FUBP1 Antibody Picoband® IHC analysis of FUBP1 using anti-FUBP1 antibody (A03126-2). FUBP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FUBP1 Antibody (A03126-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03126-2-fubp1-primary-antibodies-ihc-testing-4.jpgAnti-FUBP1 Antibody Picoband® IHC analysis of FUBP1 using anti-FUBP1 antibody (A03126-2). FUBP1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FUBP1 Antibody (A03126-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03126-2-fubp1-primary-antibodies-if-testing-5.jpgAnti-FUBP1 Antibody Picoband® IF analysis of FUBP1 using anti-FUBP1 antibody (A03126-2) and anti-Beta Tubulin antibody (M01857-3). FUBP1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FUBP1 Antibody (A03126-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1293402026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02208-1-gp6-primary-antibodies-wb-testing-1.jpgAnti-GP6 Antibody Picoband® Western blot analysis of GP6 using anti-GP6 antibody (A02208-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GP6 antigen affinity purified polyclonal antibody (A02208-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GP6 at approximately 37 kDa. The expected band size for GP6 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02208-1-gp6-primary-antibodies-fcm-testing-2.jpgAnti-GP6 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-GP6 antibody (A02208-1). Overlay histogram showing RAW264.7 cells stained with A02208-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GP6 Antibody (A02208-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293412026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03262-1-gpd1-primary-antibodies-wb-testing-1.jpgAnti-GPD1 Antibody Picoband® Western blot analysis of GPD1 using anti-GPD1 antibody (A03262-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPD1 antigen affinity purified polyclonal antibody (A03262-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GPD1 at approximately 40 kDa. The expected band size for GPD1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03262-1-gpd1-primary-antibodies-ihc-testing-2.jpgAnti-GPD1 Antibody Picoband® IHC analysis of GPD1 using anti-GPD1 antibody (A03262-1). GPD1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPD1 Antibody (A03262-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03262-1-gpd1-primary-antibodies-fcm-testing-3.jpgAnti-GPD1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-GPD1 antibody (A03262-1). Overlay histogram showing HepG2 cells stained with A03262-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GPD1 Antibody (A03262-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293422026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06269-1-gpr35-primary-antibodies-wb-testing-1.jpgAnti-GPR35 Antibody Picoband® Western blot analysis of GPR35 using anti-GPR35 antibody (A06269-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPR35 antigen affinity purified polyclonal antibody (A06269-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GPR35 at approximately 34 kDa. The expected band size for GPR35 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06269-1-gpr35-primary-antibodies-fcm-testing-2.jpgAnti-GPR35 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-GPR35 antibody (A06269-1). Overlay histogram showing HepG2 cells stained with A06269-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GPR35 Antibody (A06269-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1293432026-03-24T05:36:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05072-2-gprc5a-primary-antibodies-wb-testing-1_1.jpgAnti-RAI3/GPRC5A Antibody Picoband®Western blot analysis of GPRC5A using anti-GPRC5A antibody (A05072-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPRC5A antigen affinity purified polyclonal antibody (A05072-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GPRC5A at approximately 37-50 kDa. The expected band size for GPRC5A is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05072-2-gprc5a-primary-antibodies-ihc-testing-1.jpgAnti-RAI3/GPRC5A Antibody Picoband®IHC analysis of GPRC5A using anti-GPRC5A antibody (A05072-2). GPRC5A was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPRC5A Antibody (A05072-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05072-2-gprc5a-primary-antibodies-ihc-testing-2.jpgAnti-RAI3/GPRC5A Antibody Picoband®IHC analysis of GPRC5A using anti-GPRC5A antibody (A05072-2). GPRC5A was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPRC5A Antibody (A05072-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05072-2-gprc5a-primary-antibodies-ihc-testing-3.jpgAnti-RAI3/GPRC5A Antibody Picoband®IHC analysis of GPRC5A using anti-GPRC5A antibody (A05072-2). GPRC5A was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPRC5A Antibody (A05072-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05072-2-gprc5a-primary-antibodies-ihc-testing-4.jpgAnti-RAI3/GPRC5A Antibody Picoband®IHC analysis of GPRC5A using anti-GPRC5A antibody (A05072-2). GPRC5A was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPRC5A Antibody (A05072-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1293442026-03-10T04:39:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0651-elisa-human-afp-alpha-1-fetoprotein-picokine-elisa-kit.pngHuman AFP/Alpha-fetoprotein ELISA Kit PicoKine®Human AFP PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1293452026-03-10T04:39:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/r/protp06734-4-sdspage.jpgHuman CD23/FCER2 Recombinant Protein3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/r/protp06734-4-ba.pngHuman CD23/FCER2 Recombinant ProteinHuman IgE is coated at 5 ug/ml (100 ul/well) can bind Human CD23/FCER2. The ED50 range ≤ 15 ug/ml. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-abce1-picoband-antibody-azq6tnw3-1-boster.html2026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tnw3-1-abce1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ABCE1 Antibody Picoband® Western blot analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (AZQ6TNW3-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tnw3-1-abce1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ABCE1 Antibody Picoband® IHC analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3-1). ABCE1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCE1 Antibody (AZQ6TNW3-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tnw3-1-abce1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish ABCE1 Antibody Picoband® IHC analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3-1). ABCE1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCE1 Antibody (AZQ6TNW3-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tnw3-1-abce1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish ABCE1 Antibody Picoband® IHC analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3-1). ABCE1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCE1 Antibody (AZQ6TNW3-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tnw3-1-abce1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish ABCE1 Antibody Picoband® IHC analysis of ABCE1 using anti-ABCE1 antibody (AZQ6TNW3-1). ABCE1 was detected in a paraffin-embedded section of zebrafish retina tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCE1 Antibody (AZQ6TNW3-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-acadm-picoband-antibody-aza2cg95-boster.html2026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2cg95-acadm-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ACADM Antibody Picoband® Western blot analysis of ACADM using anti-ACADM antibody (AZA2CG95). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: whole zebrafish tissue lysates, Lane 5: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADM antigen affinity purified polyclonal antibody (AZA2CG95) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ACADM at approximately 43 kDa. The expected band size for ACADM is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-acadm-picoband-antibody-aza2cg95-1-boster.html2026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2cg95-1-acadm-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ACADM Antibody Picoband® Western blot analysis of ACADM using anti-ACADM antibody (AZA2CG95-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: whole zebrafish tissue lysates, Lane 5: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADM antigen affinity purified polyclonal antibody (AZA2CG95-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ACADM at approximately 43 kDa. The expected band size for ACADM is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-beta-actin-actb-picoband-antibody-azq7zvi7-boster.html2026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvi7-actb-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Beta Actin/ACTB Antibody Picoband® Western blot analysis of Beta Actin/ACTB using anti-Beta Actin/ACTB antibody (AZQ7ZVI7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: whole zebrafish tissue lysates, Lane 5: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (AZQ7ZVI7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvi7-actb-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Beta Actin/ACTB Antibody Picoband® IHC analysis of Beta Actin/ACTB using anti-Beta Actin/ACTB antibody (AZQ7ZVI7). Beta Actin/ACTB was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Beta Actin/ACTB Antibody (AZQ7ZVI7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-arp2-actr2-picoband-antibody-azq7sxw6-boster.html2026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxw6-actr2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ARP2/ACTR2 Antibody Picoband® Western blot analysis of ARP2/ACTR2 using anti-ARP2/ACTR2 antibody (AZQ7SXW6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: whole zebrafish tissue lysates, Lane 5: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARP2/ACTR2 antigen affinity purified polyclonal antibody (AZQ7SXW6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ARP2/ACTR2 at approximately 45 kDa. The expected band size for ARP2/ACTR2 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxw6-actr2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ARP2/ACTR2 Antibody Picoband® IHC analysis of ARP2/ACTR2 using anti-ARP2/ACTR2 antibody (AZQ7SXW6). ARP2/ACTR2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARP2/ACTR2 Antibody (AZQ7SXW6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxw6-actr2-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish ARP2/ACTR2 Antibody Picoband® IHC analysis of ARP2/ACTR2 using anti-ARP2/ACTR2 antibody (AZQ7SXW6). ARP2/ACTR2 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARP2/ACTR2 Antibody (AZQ7SXW6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxw6-actr2-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish ARP2/ACTR2 Antibody Picoband® IHC analysis of ARP2/ACTR2 using anti-ARP2/ACTR2 antibody (AZQ7SXW6). ARP2/ACTR2 was detected in a paraffin-embedded section of zebrafish retina tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARP2/ACTR2 Antibody (AZQ7SXW6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxw6-actr2-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish ARP2/ACTR2 Antibody Picoband® IHC analysis of ARP2/ACTR2 using anti-ARP2/ACTR2 antibody (AZQ7SXW6). ARP2/ACTR2 was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARP2/ACTR2 Antibody (AZQ7SXW6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-adrm1-picoband-antibody-azq6nz09-boster.html2026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nz09-adrm1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ADRM1 Antibody Picoband® Western blot analysis of ADRM1 using anti-ADRM1 antibody (AZQ6NZ09). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: whole zebrafish tissue lysates, Lane 5: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRM1 antigen affinity purified polyclonal antibody (AZQ6NZ09) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ADRM1 at approximately 42 kDa. The expected band size for ADRM1 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nz09-adrm1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ADRM1 Antibody Picoband® IHC analysis of ADRM1 using anti-ADRM1 antibody (AZQ6NZ09). ADRM1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADRM1 Antibody (AZQ6NZ09) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-aff4-picoband-antibody-aza0a8n7tei3-boster.html2026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8n7tei3-aff4-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish AFF4 Antibody Picoband® Western blot analysis of AFF4 using anti-AFF4 antibody (AZA0A8N7TEI3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFF4 antigen affinity purified polyclonal antibody (AZA0A8N7TEI3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AFF4 at approximately 170 kDa. The expected band size for AFF4 is at 127 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ago1-picoband-antibody-azk4i6k9-boster.html2026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azk4i6k9-ago1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish AGO1 Antibody Picoband® Western blot analysis of AGO1 using anti-AGO1 antibody (AZK4I6K9). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGO1 antigen affinity purified polyclonal antibody (AZK4I6K9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AGO1 at approximately 97 kDa. The expected band size for AGO1 is at 97 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-akt1-2-3-picoband-antibody-azq802y3-boster.html2026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq802y3-akt1-2-3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish AKT1/2/3 Antibody Picoband® Western blot analysis of AKT1/2/3 using anti-AKT1/2/3 antibody (AZQ802Y3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1/2/3 antigen affinity purified polyclonal antibody (AZQ802Y3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AKT1/2/3 at approximately 62 kDa. The expected band size for AKT1/2/3 is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-akt3-picoband-antibody-azd9il79-boster.html2026-03-17T05:16:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azd9il79-akt3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish AKT3 Antibody Picoband® Western blot analysis of AKT3 using anti-AKT3 antibody (AZD9IL79). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: female zebrafish viscera tissue lysates, Lane 2: male zebrafish viscera tissue lysates, Lane 3: whole zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (AZD9IL79) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AKT3 at approximately 56 kDa. The expected band size for AKT3 is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-p5cs-aldh18a1-antibody.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza4igc8-aldh18a1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish P5CS/ALDH18A1 Antibody IHC analysis of P5CS/ALDH18A1 using anti-P5CS/ALDH18A1 antibody (AZA4IGC8). P5CS/ALDH18A1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P5CS/ALDH18A1 Antibody (AZA4IGC8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-arid2-picoband-antibody-aza0jpe6-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0jpe6-arid2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ARID2 Antibody Picoband® Western blot analysis of ARID2 using anti-ARID2 antibody (AZA0JPE6). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARID2 antigen affinity purified polyclonal antibody (AZA0JPE6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ARID2 at approximately 240 kDa. The expected band size for ARID2 is at 197 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ash2l-antibody.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2b2e1-ash2l-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish ASH2L Antibody IHC analysis of ASH2L using anti-ASH2L antibody (AZA0A8M2B2E1). ASH2L was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ASH2L Antibody (AZA0A8M2B2E1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2b2e1-ash2l-primary-antibodies-if-testing-2.jpgAnti-Zebrafish ASH2L Antibody IF analysis of ASH2L using anti-ASH2L antibody (AZA0A8M2B2E1). ASH2L was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ASH2L Antibody (AZA0A8M2B2E1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®594-conjugated Anti-rabbit IgG Secondary Antibody (red) (Catalog # BA1142). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2b2e1-ash2l-primary-antibodies-if-testing-3.jpgAnti-Zebrafish ASH2L Antibody IF analysis of ASH2L using anti-ASH2L antibody (AZA0A8M2B2E1). ASH2L was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ASH2L Antibody (AZA0A8M2B2E1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®594-conjugated Anti-rabbit IgG Secondary Antibody (red) (Catalog # BA1142). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2b2e1-ash2l-primary-antibodies-if-testing-4.jpgAnti-Zebrafish ASH2L Antibody IF analysis of ASH2L using anti-ASH2L antibody (AZA0A8M2B2E1). ASH2L was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ASH2L Antibody (AZA0A8M2B2E1) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®594-conjugated Anti-rabbit IgG Secondary Antibody (red) (Catalog # BA1142). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-atpb-atp5f1b-picoband-antibody-aza8wgc6-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8wgc6-atp5f1b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ATPB/ATP5F1B Antibody Picoband® Western blot analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody (AZA8WGC6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: whole zebrafish tissue lysates, Lane 5: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATPB/ATP5F1B antigen affinity purified polyclonal antibody (AZA8WGC6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATPB/ATP5F1B at approximately 50 kDa. The expected band size for ATPB/ATP5F1B is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8wgc6-atp5f1b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ATPB/ATP5F1B Antibody Picoband® IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody (AZA8WGC6). ATPB/ATP5F1B was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATPB/ATP5F1B Antibody (AZA8WGC6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8wgc6-atp5f1b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish ATPB/ATP5F1B Antibody Picoband® IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody (AZA8WGC6). ATPB/ATP5F1B was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATPB/ATP5F1B Antibody (AZA8WGC6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8wgc6-atp5f1b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish ATPB/ATP5F1B Antibody Picoband® IHC analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody (AZA8WGC6). ATPB/ATP5F1B was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATPB/ATP5F1B Antibody (AZA8WGC6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8wgc6-atp5f1ba-primary-antibodies-if-testing-5.jpgAnti-Zebrafish ATPB/ATP5F1B Antibody Picoband® IF analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody (AZA8WGC6). ATPB/ATP5F1B was detected in paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-ATPB/ATP5F1B Antibody (AZA8WGC6) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-atp5g1-2-3-picoband-antibody-azq6iqn6-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqn6-atp5mc1-2-3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ATP5G1/2/3 Antibody Picoband® Western blot analysis of ATP5G1/2/3 using anti-ATP5G1/2/3 antibody (AZQ6IQN6). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: whole zebrafish tissue lysates, Lane 5: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5G1/2/3 antigen affinity purified polyclonal antibody (AZQ6IQN6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATP5G1/2/3 at approximately 10 kDa. The expected band size for ATP5G1/2/3 is at 10 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqn6-atp5mc1-2-3-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ATP5G1/2/3 Antibody Picoband® IHC analysis of ATP5G1/2/3 using anti-ATP5G1/2/3 antibody (AZQ6IQN6). ATP5G1/2/3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5G1/2/3 Antibody (AZQ6IQN6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqn6-atp5g1-2-3a-primary-antibodies-if-testing-3.jpgAnti-Zebrafish ATP5G1/2/3 Antibody Picoband® IF analysis of ATP5G1/2/3 using anti-ATP5G1/2/3 antibody (AZQ6IQN6). ATP5G1/2/3 was detected in paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-ATP5G1/2/3 Antibody (AZQ6IQN6) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-atp6v1a-picoband-antibody-azq7sy46-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sy46-atp6v1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ATP6V1A Antibody Picoband® Western blot analysis of ATP6V1A using anti-ATP6V1A antibody (AZQ7SY46). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1A antigen affinity purified polyclonal antibody (AZQ7SY46) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATP6V1A at approximately 68 kDa. The expected band size for ATP6V1A is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sy46-atp6v1a-primary-antibodies-if-testing-2.jpgAnti-Zebrafish ATP6V1A Antibody Picoband® IF analysis of ATP6V1A using anti-ATP6V1A antibody (AZQ7SY46). ATP6V1A was detected in paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-ATP6V1A Antibody (AZQ7SY46) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-atp6v1a-picoband-antibody-aze7fcd8-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7fcd8-atp6v1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ATP6V1A Antibody Picoband® Western blot analysis of ATP6V1A using anti-ATP6V1A antibody (AZE7FCD8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1A antigen affinity purified polyclonal antibody (AZE7FCD8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATP6V1A at approximately 68 kDa. The expected band size for ATP6V1A is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7fcd8-atp6v1a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (AZE7FCD8). ATP6V1A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (AZE7FCD8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7fcd8-atp6v1a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (AZE7FCD8). ATP6V1A was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (AZE7FCD8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7fcd8-atp6v1a-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (AZE7FCD8). ATP6V1A was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (AZE7FCD8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7fcd8-atp6v1a-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (AZE7FCD8). ATP6V1A was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (AZE7FCD8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7fcd8-atp6v1a-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (AZE7FCD8). ATP6V1A was detected in a paraffin-embedded section of zebrafish spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (AZE7FCD8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7fcd8-atp6v1a-primary-antibodies-ihc-testing-7.jpgAnti-Zebrafish ATP6V1A Antibody Picoband® IHC analysis of ATP6V1A using anti-ATP6V1A antibody (AZE7FCD8). ATP6V1A was detected in a paraffin-embedded section of zebrafish intestinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1A Antibody (AZE7FCD8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-atp6v1b2-picoband-antibody-azq8qha6-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8qha6-atp6v1b2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ATP6V1B2 Antibody Picoband® Western blot analysis of ATP6V1B2 using anti-ATP6V1B2 antibody (AZQ8QHA6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1B2 antigen affinity purified polyclonal antibody (AZQ8QHA6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATP6V1B2 at approximately 57 kDa. The expected band size for ATP6V1B2 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8qha6-atp6v1b2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ATP6V1B2 Antibody Picoband® IHC analysis of ATP6V1B2 using anti-ATP6V1B2 antibody (AZQ8QHA6). ATP6V1B2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1B2 Antibody (AZQ8QHA6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-bap1-antibody.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l2g3-bap1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish BAP1 Antibody IHC analysis of BAP1 using anti-BAP1 antibody (AZA1L2G3). BAP1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BAP1 Antibody (AZA1L2G3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l2g3-bap1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish BAP1 Antibody IHC analysis of BAP1 using anti-BAP1 antibody (AZA1L2G3). BAP1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BAP1 Antibody (AZA1L2G3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-c9orf72-picoband-antibody-azq6tlh8-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tlh8-c9orf72-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish C9orf72 Antibody Picoband® Western blot analysis of C9orf72 using anti-C9orf72 antibody (AZQ6TLH8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C9orf72 antigen affinity purified polyclonal antibody (AZQ6TLH8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for C9orf72 at approximately 54 kDa. The expected band size for C9orf72 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tlh8-c9orf72-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish C9orf72 Antibody Picoband® IHC analysis of C9orf72 using anti-C9orf72 antibody (AZQ6TLH8). C9orf72 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C9orf72 Antibody (AZQ6TLH8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cask-antibody.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq910a4-cask-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish CASK Antibody IHC analysis of CASK using anti-CASK antibody (AZQ910A4). CASK was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CASK Antibody (AZQ910A4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq910a4-cask-primary-antibodies-if-testing-2..jpgAnti-Zebrafish CASK Antibody IF analysis of CASK using anti-CASK antibody (AZQ910A4). CASK was detected in paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-CASK Antibody (AZQ910A4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cbs-picoband-antibody-aza9jt04-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza9jt04-cbs-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CBS Antibody Picoband® Western blot analysis of CBS using anti-CBS antibody (AZA9JT04). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBS antigen affinity purified polyclonal antibody (AZA9JT04) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CBS at approximately 40 kDa. The expected band size for CBS is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza9jt04-cbs-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CBS Antibody Picoband® IHC analysis of CBS using anti-CBS antibody (AZA9JT04). CBS was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CBS Antibody (AZA9JT04) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cct3-picoband-antibody-azq8jhi7-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8jhi7-cct3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CCT3 Antibody Picoband® Western blot analysis of CCT3 using anti-CCT3 antibody (AZQ8JHI7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT3 antigen affinity purified polyclonal antibody (AZQ8JHI7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CCT3 at approximately 56 kDa. The expected band size for CCT3 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8jhi7-cct3-primary-antibodies-if-testing-2.jpgAnti-Zebrafish CCT3 Antibody Picoband® IF analysis of CCT3 using anti-CCT3 antibody (AZQ8JHI7). CCT3 was detected in paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-CCT3 Antibody (AZQ8JHI7) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cct4-picoband-antibody-azq6ph46-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph46-cct4-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CCT4 Antibody Picoband® Western blot analysis of CCT4 using anti-CCT4 antibody (AZQ6PH46). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT4 antigen affinity purified polyclonal antibody (AZQ6PH46) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CCT4 at approximately 58 kDa. The expected band size for CCT4 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph46-cct4-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CCT4 Antibody Picoband® IHC analysis of CCT4 using anti-CCT4 antibody (AZQ6PH46). CCT4 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCT4 Antibody (AZQ6PH46) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph46-cct4-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish CCT4 Antibody Picoband® IHC analysis of CCT4 using anti-CCT4 antibody (AZQ6PH46). CCT4 was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCT4 Antibody (AZQ6PH46) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph46-cct4-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish CCT4 Antibody Picoband® IHC analysis of CCT4 using anti-CCT4 antibody (AZQ6PH46). CCT4 was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCT4 Antibody (AZQ6PH46) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph46-cct4a-primary-antibodies-if-testing-5.jpgAnti-Zebrafish CCT4 Antibody Picoband® IF analysis of CCT4 using anti-CCT4 antibody (AZQ6PH46). CCT4 was detected in paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-CCT4 Antibody (AZQ6PH46) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cct7-antibody.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8jhg7-cct7-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish CCT7 Antibody IHC analysis of CCT7 using anti-CCT7 antibody (AZQ8JHG7). CCT7 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCT7 Antibody (AZQ8JHG7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-flotillin-1-flot1-picoband-antibody-azf1qew4-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qew4-flot1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Flotillin 1/FLOT1 Antibody Picoband® Western blot analysis of Flotillin 1/FLOT1 using anti-Flotillin 1/FLOT1 antibody (AZF1QEW4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flotillin 1/FLOT1 antigen affinity purified polyclonal antibody (AZF1QEW4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Flotillin 1/FLOT1 at approximately 47 kDa. The expected band size for Flotillin 1/FLOT1 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qew4-flot1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Flotillin 1/FLOT1 Antibody Picoband® IHC analysis of Flotillin 1/FLOT1 using anti-Flotillin 1/FLOT1 antibody (AZF1QEW4). Flotillin 1/FLOT1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Flotillin 1/FLOT1 Antibody (AZF1QEW4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qew4-flot1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish Flotillin 1/FLOT1 Antibody Picoband® IHC analysis of Flotillin 1/FLOT1 using anti-Flotillin 1/FLOT1 antibody (AZF1QEW4). Flotillin 1/FLOT1 was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Flotillin 1/FLOT1 Antibody (AZF1QEW4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gnaq-picoband-antibody-azb8jlj7-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jlj7-gnaq-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GNAQ Antibody Picoband® Western blot analysis of GNAQ using anti-GNAQ antibody (AZB8JLJ7). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAQ antigen affinity purified polyclonal antibody (AZB8JLJ7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GNAQ at approximately 42 kDa. The expected band size for GNAQ is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jlj7-gnaq-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GNAQ Antibody Picoband® IHC analysis of GNAQ using anti-GNAQ antibody (AZB8JLJ7). GNAQ was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNAQ Antibody (AZB8JLJ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jlj7-gnaq-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish GNAQ Antibody Picoband® IHC analysis of GNAQ using anti-GNAQ antibody (AZB8JLJ7). GNAQ was detected in a paraffin-embedded section of zebrafish esophagus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNAQ Antibody (AZB8JLJ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hprt1-picoband-antibody-azq7zv49-boster.html2026-03-17T05:16:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv49-hprt1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HPRT1 Antibody Picoband® Western blot analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: whole female zebrafish tissue lysates, Lane 5: whole male zebrafish tissue lysates, Lane 6: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPRT1 antigen affinity purified polyclonal antibody (AZQ7ZV49) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HPRT1 at approximately 27 kDa. The expected band size for HPRT1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv49-hprt1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HPRT1 Antibody Picoband® IHC analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49). HPRT1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HPRT1 Antibody (AZQ7ZV49) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv49-hprt1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HPRT1 Antibody Picoband® IHC analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49). HPRT1 was detected in a paraffin-embedded section of zebrafish retina tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HPRT1 Antibody (AZQ7ZV49) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hsc70-hspa8-picoband-antibody-azq90473-boster.html2026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90473-hspa8-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HSC70/HSPA8 Antibody Picoband® Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (AZQ90473). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: whole female zebrafish tissue lysates, Lane 5: whole male zebrafish tissue lysates, Lane 6: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSC70/HSPA8 antigen affinity purified polyclonal antibody (AZQ90473) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HSC70/HSPA8 at approximately 71 kDa. The expected band size for HSC70/HSPA8 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90473-hspa8-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HSC70/HSPA8 Antibody Picoband® IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (AZQ90473). HSC70/HSPA8 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSC70/HSPA8 Antibody (AZQ90473) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90473-hspa8-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HSC70/HSPA8 Antibody Picoband® IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (AZQ90473). HSC70/HSPA8 was detected in a paraffin-embedded section of zebrafish retina tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSC70/HSPA8 Antibody (AZQ90473) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ilk-picoband-antibody-azq6phd6-boster.html2026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phd6-ilk-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ILK Antibody Picoband® Western blot analysis of ILK using anti-ILK antibody (AZQ6PHD6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILK antigen affinity purified polyclonal antibody (AZQ6PHD6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ILK at approximately 51 kDa. The expected band size for ILK is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-kv1-2-kcna2-picoband-antibody-aze7f8m2-boster.html2026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f8m2-kcna2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Kv1.2/KCNA2 Antibody Picoband® Western blot analysis of Kv1.2/KCNA2 using anti-Kv1.2/KCNA2 antibody (AZE7F8M2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kv1.2/KCNA2 antigen affinity purified polyclonal antibody (AZE7F8M2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Kv1.2/KCNA2 at approximately 57 kDa. The expected band size for Kv1.2/KCNA2 is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-kif3a-picoband-antibody-aze9qb71-boster.html2026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze9qb71-kif3a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish KIF3A Antibody Picoband® Western blot analysis of KIF3A using anti-KIF3A antibody (AZE9QB71). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: whole zebrafish tissue lysates, Lane 5: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF3A antigen affinity purified polyclonal antibody (AZE9QB71) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KIF3A at approximately 70 kDa. The expected band size for KIF3A is at 80 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pbef-nampt-antibody.html2026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f8t6-nampt-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PBEF/NAMPT Antibody IHC analysis of PBEF/NAMPT using anti-PBEF/NAMPT antibody (AZE7F8T6). PBEF/NAMPT was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PBEF/NAMPT Antibody (AZE7F8T6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-alpha-parvin-actopaxin-parva-picoband-antibody-azq6drm4-boster.html2026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6drm4-parva-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Alpha Parvin/Actopaxin/PARVA Antibody Picoband® Western blot analysis of Alpha Parvin/Actopaxin/PARVA using anti-Alpha Parvin/Actopaxin/PARVA antibody (AZQ6DRM4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: whole female zebrafish tissue lysates, Lane 5: whole male zebrafish tissue lysates, Lane 6: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Parvin/Actopaxin/PARVA antigen affinity purified polyclonal antibody (AZQ6DRM4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Alpha Parvin/Actopaxin/PARVA at approximately 42 kDa. The expected band size for Alpha Parvin/Actopaxin/PARVA is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pgk1-picoband-antibody-azq7zv29-boster.html2026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv29-pgk1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PGK1 Antibody Picoband® Western blot analysis of PGK1 using anti-PGK1 antibody (AZQ7ZV29). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: female zebrafish viscera tissue lysates, Lane 3: male zebrafish viscera tissue lysates, Lane 4: whole female zebrafish tissue lysates, Lane 5: whole male zebrafish tissue lysates, Lane 6: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGK1 antigen affinity purified polyclonal antibody (AZQ7ZV29) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PGK1 at approximately 42 kDa. The expected band size for PGK1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv29-pgk1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PGK1 Antibody Picoband® IHC analysis of PGK1 using anti-PGK1 antibody (AZQ7ZV29). PGK1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGK1 Antibody (AZQ7ZV29) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv29-pgk1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PGK1 Antibody Picoband® IHC analysis of PGK1 using anti-PGK1 antibody (AZQ7ZV29). PGK1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGK1 Antibody (AZQ7ZV29) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ppp2ca-picoband-antibody-azq567y8-boster.html2026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq567y8-ppp2ca-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PPP2CA Antibody Picoband® Western blot analysis of PPP2CA using anti-PPP2CA antibody (AZQ567Y8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2CA antigen affinity purified polyclonal antibody (AZQ567Y8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPP2CA at approximately 36 kDa. The expected band size for PPP2CA is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq567y8-ppp2ca-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PPP2CA Antibody Picoband® IHC analysis of PPP2CA using anti-PPP2CA antibody (AZQ567Y8). PPP2CA was detected in a paraffin-embedded section of zebrafish retina tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2CA Antibody (AZQ567Y8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq567y8-ppp2ca-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PPP2CA Antibody Picoband® IHC analysis of PPP2CA using anti-PPP2CA antibody (AZQ567Y8). PPP2CA was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2CA Antibody (AZQ567Y8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq567y8-ppp2ca-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish PPP2CA Antibody Picoband® IHC analysis of PPP2CA using anti-PPP2CA antibody (AZQ567Y8). PPP2CA was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2CA Antibody (AZQ567Y8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ptp4a2-picoband-antibody-azq5u3r3-boster.html2026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u3r3-ptp4a2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PTP4A2 Antibody Picoband® Western blot analysis of PTP4A2 using anti-PTP4A2 antibody (AZQ5U3R3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTP4A2 antigen affinity purified polyclonal antibody (AZQ5U3R3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTP4A2 at approximately 19 kDa. The expected band size for PTP4A2 is at 19 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-shp2-ptpn11-picoband-antibody-azq7zw17-boster.html2026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zw17-ptpn11-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SHP2/PTPN11 Antibody Picoband® Western blot analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (AZQ7ZW17). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2/PTPN11 antigen affinity purified polyclonal antibody (AZQ7ZW17) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SHP2/PTPN11 at approximately 70 kDa. The expected band size for SHP2/PTPN11 is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rab10-picoband-antibody-azq6dgv5-boster.html2026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dgv5-rab10-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RAB10 Antibody Picoband® Western blot analysis of RAB10 using anti-RAB10 antibody (AZQ6DGV5). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB10 antigen affinity purified polyclonal antibody (AZQ6DGV5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB10 at approximately 22 kDa. The expected band size for RAB10 is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rac1-picoband-antibody-azq7zsz9-boster.html2026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zsz9-rac1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RAC1 Antibody Picoband® Western blot analysis of RAC1 using anti-RAC1 antibody (AZQ7ZSZ9). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAC1 antigen affinity purified polyclonal antibody (AZQ7ZSZ9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAC1 at approximately 21 kDa. The expected band size for RAC1 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zsz9-rac1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish RAC1 Antibody Picoband® IHC analysis of RAC1 using anti-RAC1 antibody (AZQ7ZSZ9). RAC1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAC1 Antibody (AZQ7ZSZ9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/polyclonal-anti-cav1-3-cacna1da-antibody-dz33931-boster.html2026-03-17T05:16:31+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-eif4e1c-antibody.html2026-03-17T05:16:31+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-eif4ea-antibody.html2026-03-17T05:16:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1293942026-03-10T04:40:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1293952026-03-10T04:40:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1293962026-04-06T05:04:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1293972026-03-10T04:40:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1293982026-03-10T04:40:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1293992026-03-10T04:40:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1294002026-03-10T04:40:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1294012026-03-10T04:40:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1294022026-03-10T04:40:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1294042026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03009-2-adamts1-primary-antibodies-wb-testing-1.jpgAnti-ADAMTS1 Antibody Picoband® Western blot analysis of ADAMTS1 using anti-ADAMTS1 antibody (A03009-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS1 antigen affinity purified polyclonal antibody (A03009-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ADAMTS1 at approximately 105 kDa. The expected band size for ADAMTS1 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03009-2-adamts1-primary-antibodies-ihc-testing-2.jpgAnti-ADAMTS1 Antibody Picoband® IHC analysis of ADAMTS1 using anti-ADAMTS1 antibody (A03009-2). ADAMTS1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADAMTS1 Antibody (A03009-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03009-2-adamts1-primary-antibodies-fcm-testing-3.jpgAnti-ADAMTS1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-ADAMTS1 antibody (A03009-2). Overlay histogram showing 293T cells stained with A03009-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ADAMTS1 Antibody (A03009-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294052026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16667-spata21-primary-antibodies-wb-testing-1.jpgAnti-SPATA21 Antibody Picoband® Western blot analysis of SPATA21 using anti-SPATA21 antibody (A16667). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA21 antigen affinity purified polyclonal antibody (A16667) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPATA21 at approximately 55 kDa. The expected band size for SPATA21 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16667-spata21-primary-antibodies-fcm-testing-2.jpgAnti-SPATA21 Antibody Picoband® Flow Cytometry analysis of RT4 cells using anti-SPATA21 antibody (A16667). Overlay histogram showing RT4 cells stained with A16667 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPATA21 Antibody (A16667, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294062026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09473-2-cby2-primary-antibodies-wb-testing-1.jpgAnti-CBY2 Antibody Picoband® Western blot analysis of CBY2 using anti-CBY2 antibody (A09473-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBY2 antigen affinity purified polyclonal antibody (A09473-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CBY2 at approximately 52 kDa. The expected band size for CBY2 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09473-2-cby2-primary-antibodies-fcm-testing-2.jpgAnti-CBY2 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-CBY2 antibody (A09473-2). Overlay histogram showing Jurkat cells stained with A09473-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CBY2 Antibody (A09473-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294072026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04719-3-spo11-primary-antibodies-wb-testing-1.jpgAnti-SPO11 Antibody Picoband® Western blot analysis of SPO11 using anti-SPO11 antibody (A04719-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPO11 antigen affinity purified polyclonal antibody (A04719-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPO11 at approximately 70 kDa. The expected band size for SPO11 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04719-3-spo11-primary-antibodies-if-testing-2.jpgAnti-SPO11 Antibody Picoband® IF analysis of SPO11 using anti-SPO11 antibody (A04719-3) and anti-Beta Tubulin antibody (M01857-3). SPO11 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SPO11 Antibody (A04719-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1294082026-03-17T05:16:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08811-1-spock2-primary-antibodies-wb-testing-1.jpgAnti-SPOCK2 Antibody Picoband® Western blot analysis of SPOCK2 using anti-SPOCK2 antibody (A08811-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPOCK2 antigen affinity purified polyclonal antibody (A08811-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPOCK2 at approximately 55 kDa. The expected band size for SPOCK2 is at 47 kDa. https://www.bosterbio.com/catalog/product/view/id/1294092026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12965-1-spry3-primary-antibodies-wb-testing-1.jpgAnti-SPRY3 Antibody Picoband® Western blot analysis of SPRY3 using anti-SPRY3 antibody (A12965-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPRY3 antigen affinity purified polyclonal antibody (A12965-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPRY3 at approximately 36 kDa. The expected band size for SPRY3 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12965-1-spry3-primary-antibodies-fcm-testing-2.jpgAnti-SPRY3 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-SPRY3 antibody (A12965-1). Overlay histogram showing MCF-7 cells stained with A12965-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPRY3 Antibody (A12965-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294102026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10914-2-ggn-primary-antibodies-wb-testing-1.jpgAnti-GGN Antibody Picoband® Western blot analysis of GGN using anti-GGN antibody (A10914-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGN antigen affinity purified polyclonal antibody (A10914-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GGN at approximately 67 kDa. The expected band size for GGN is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10914-2-ggn-primary-antibodies-fcm-testing-2.jpgAnti-GGN Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-GGN antibody (A10914-2). Overlay histogram showing SH-SY5Y cells stained with A10914-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GGN Antibody (A10914-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294112026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04423-2-gstm2-primary-antibodies-wb-testing-1.jpgAnti-GSTM2 Antibody Picoband® Western blot analysis of GSTM2 using anti-GSTM2 antibody (A04423-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTM2 antigen affinity purified polyclonal antibody (A04423-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSTM2 at approximately 26 kDa. The expected band size for GSTM2 is at 26 kDa. https://www.bosterbio.com/catalog/product/view/id/1294122026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04423-3-gstm2-primary-antibodies-wb-testing-1.jpgAnti-GSTM2 Antibody Picoband® Western blot analysis of GSTM2 using anti-GSTM2 antibody (A04423-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTM2 antigen affinity purified polyclonal antibody (A04423-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSTM2 at approximately 26 kDa. The expected band size for GSTM2 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04423-3-gstm2-primary-antibodies-ihc-testing-2.jpgAnti-GSTM2 Antibody Picoband® IHC analysis of GSTM2 using anti-GSTM2 antibody (A04423-3). GSTM2 was detected in a paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTM2 Antibody (A04423-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1294132026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06456-1-pxdn-primary-antibodies-wb-testing-1.jpgAnti-PXDN Antibody Picoband® Western blot analysis of PXDN using anti-PXDN antibody (A06456-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PXDN antigen affinity purified polyclonal antibody (A06456-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PXDN at approximately 165 kDa. The expected band size for PXDN is at 165 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06456-1-pxdn-primary-antibodies-ihc-testing-2.jpgAnti-PXDN Antibody Picoband® IHC analysis of PXDN using anti-PXDN antibody (A06456-1). PXDN was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PXDN Antibody (A06456-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06456-1-pxdn-primary-antibodies-ihc-testing-3.jpgAnti-PXDN Antibody Picoband® IHC analysis of PXDN using anti-PXDN antibody (A06456-1). PXDN was detected in a paraffin-embedded section of human clear cell renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PXDN Antibody (A06456-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1294142026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11433-2-supt20h-primary-antibodies-wb-testing-1.jpgAnti-SUPT20H Antibody Picoband® Western blot analysis of SUPT20H using anti-SUPT20H antibody (A11433-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUPT20H antigen affinity purified polyclonal antibody (A11433-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUPT20H at approximately 86 kDa. The expected band size for SUPT20H is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11433-2-supt20h-primary-antibodies-if-testing-2.jpgAnti-SUPT20H Antibody Picoband® IF analysis of SUPT20H using anti-SUPT20H antibody (A11433-2) and anti-Beta Tubulin antibody (M01857-3). SUPT20H was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SUPT20H Antibody (A11433-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1294152026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11596-3-gk2-primary-antibodies-wb-testing-1.jpgAnti-GK2 Antibody Picoband® Western blot analysis of GK2 using anti-GK2 antibody (A11596-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GK2 antigen affinity purified polyclonal antibody (A11596-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GK2 at approximately 61 kDa. The expected band size for GK2 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11596-3-gk2-primary-antibodies-if-testing-2.jpgAnti-GK2 Antibody Picoband® IF analysis of GK2 using anti-GK2 antibody (A11596-3). GK2 was detected in an immunocytochemical section of MG63 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GK2 Antibody (A11596-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11596-3-gk2-primary-antibodies-fcm-testing-3.jpgAnti-GK2 Antibody Picoband® Flow Cytometry analysis of SH-SY5Y cells using anti-GK2 antibody (A11596-3). Overlay histogram showing SH-SY5Y cells stained with A11596-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GK2 Antibody (A11596-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294162026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13528-2-qrsl1-primary-antibodies-wb-testing-1.jpgAnti-QRSL1 Antibody Picoband® Western blot analysis of QRSL1 using anti-QRSL1 antibody (A13528-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QRSL1 antigen affinity purified polyclonal antibody (A13528-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for QRSL1 at approximately 57 kDa. The expected band size for QRSL1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13528-2-qrsl1-primary-antibodies-ihc-testing-2.jpgAnti-QRSL1 Antibody Picoband® IHC analysis of QRSL1 using anti-QRSL1 antibody (A13528-2). QRSL1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-QRSL1 Antibody (A13528-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13528-2-qrsl1-primary-antibodies-ihc-testing-3.jpgAnti-QRSL1 Antibody Picoband® IHC analysis of QRSL1 using anti-QRSL1 antibody (A13528-2). QRSL1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-QRSL1 Antibody (A13528-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13528-2-qrsl1-primary-antibodies-fcm-testing-4.jpgAnti-QRSL1 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-QRSL1 antibody (A13528-2). Overlay histogram showing MCF-7 cells stained with A13528-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-QRSL1 Antibody (A13528-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294172026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13920-2-syap1-primary-antibodies-wb-testing-1.jpgAnti-SYAP1 Antibody Picoband® Western blot analysis of SYAP1 using anti-SYAP1 antibody (A13920-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYAP1 antigen affinity purified polyclonal antibody (A13920-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYAP1 at approximately 50 kDa. The expected band size for SYAP1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13920-2-syap1-primary-antibodies-ihc-testing-2.jpgAnti-SYAP1 Antibody Picoband® IHC analysis of SYAP1 using anti-SYAP1 antibody (A13920-2). SYAP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SYAP1 Antibody (A13920-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13920-2-syap1-primary-antibodies-ihc-testing-3.jpgAnti-SYAP1 Antibody Picoband® IHC analysis of SYAP1 using anti-SYAP1 antibody (A13920-2). SYAP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SYAP1 Antibody (A13920-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13920-2-syap1-primary-antibodies-fcm-testing-4.jpgAnti-SYAP1 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-SYAP1 antibody (A13920-2). Overlay histogram showing HepG2 cells stained with A13920-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYAP1 Antibody (A13920-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294182026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10958-1-sv2c-primary-antibodies-wb-testing-1.jpgAnti-SV2C Antibody Picoband® Western blot analysis of SV2C using anti-SV2C antibody (A10958-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human RT-4 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neoro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SV2C antigen affinity purified polyclonal antibody (A10958-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SV2C at approximately 90 kDa. The expected band size for SV2C is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10958-1-sv2c-primary-antibodies-fcm-testing-2.jpgAnti-SV2C Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-SV2C antibody (A10958-1). Overlay histogram showing Jurkat cells stained with A10958-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SV2C Antibody (A10958-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294192026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08716-3-wdr26-primary-antibodies-wb-testing-1.jpgAnti-WDR26 Antibody Picoband® Western blot analysis of WDR26 using anti-WDR26 antibody (A08716-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR26 antigen affinity purified polyclonal antibody (A08716-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for WDR26 at approximately 72 kDa. The expected band size for WDR26 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08716-3-wdr26-primary-antibodies-if-testing-2.jpgAnti-WDR26 Antibody Picoband® IF analysis of WDR26 using anti-WDR26 antibody (A08716-3) and anti-Beta Tubulin antibody (M01857-3). WDR26 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-WDR26 Antibody (A08716-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08716-3-wdr26-primary-antibodies-fcm-testing-3.jpgAnti-WDR26 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-WDR26 antibody (A08716-3). Overlay histogram showing K562 cells stained with A08716-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR26 Antibody (A08716-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294202026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11818-1-rnf220-primary-antibodies-wb-testing-1.jpgAnti-RNF220 Antibody Picoband® Western blot analysis of RNF220 using anti-RNF220 antibody (A11818-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF220 antigen affinity purified polyclonal antibody (A11818-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RNF220 at approximately 63 kDa. The expected band size for RNF220 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11818-1-rnf220-primary-antibodies-ihc-testing-2.jpgAnti-RNF220 Antibody Picoband® IHC analysis of RNF220 using anti-RNF220 antibody (A11818-1). RNF220 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNF220 Antibody (A11818-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11818-1-rnf220-primary-antibodies-ihc-testing-3.jpgAnti-RNF220 Antibody Picoband® IHC analysis of RNF220 using anti-RNF220 antibody (A11818-1). RNF220 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNF220 Antibody (A11818-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11818-1-rnf220-primary-antibodies-ihc-testing-4.jpgAnti-RNF220 Antibody Picoband® IHC analysis of RNF220 using anti-RNF220 antibody (A11818-1). RNF220 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNF220 Antibody (A11818-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11818-1-rnf220-primary-antibodies-if-testing-5.jpgAnti-RNF220 Antibody Picoband® IF analysis of RNF220 using anti-RNF220 antibody (A11818-1) and anti-Beta Tubulin antibody (M01857-3). RNF220 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RNF220 Antibody (A11818-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11818-1-rnf220-primary-antibodies-fcm-testing-6.jpgAnti-RNF220 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-RNF220 antibody (A11818-1). Overlay histogram showing 293T cells stained with A11818-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF220 Antibody (A11818-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294212026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15752-1-wdr37-primary-antibodies-wb-testing-1.jpgAnti-WDR37 Antibody Picoband® Western blot analysis of WDR37 using anti-WDR37 antibody (A15752-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR37 antigen affinity purified polyclonal antibody (A15752-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for WDR37 at approximately 55 kDa. The expected band size for WDR37 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15752-1-wdr37-primary-antibodies-fcm-testing-2.jpgAnti-WDR37 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-WDR37 antibody (A15752-1). Overlay histogram showing HepG2 cells stained with A15752-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR37 Antibody (A15752-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294222026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14373-1-syt12-primary-antibodies-wb-testing-1.jpgAnti-Synaptotagmin-12/SYT12 Antibody Picoband® Western blot analysis of Synaptotagmin-12/SYT12 using anti-Synaptotagmin-12/SYT12 antibody (A14373-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptotagmin-12/SYT12 antigen affinity purified polyclonal antibody (A14373-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Synaptotagmin-12/SYT12 at approximately 47 kDa. The expected band size for Synaptotagmin-12/SYT12 is at 47 kDa. https://www.bosterbio.com/catalog/product/view/id/1294232026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11587-1-syt17-primary-antibodies-wb-testing-1.jpgAnti-SYT17 Antibody Picoband® Western blot analysis of SYT17 using anti-SYT17 antibody (A11587-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYT17 antigen affinity purified polyclonal antibody (A11587-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYT17 at approximately 75 kDa. The expected band size for SYT17 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11587-1-syt17-primary-antibodies-if-testing-2.jpgAnti-SYT17 Antibody Picoband® IF analysis of SYT17 using anti-SYT17 antibody (A11587-1) and anti-Beta Tubulin antibody (M01857-3). SYT17 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SYT17 Antibody (A11587-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1294242026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12369-oard1-primary-antibodies-wb-testing-1.jpgAnti-C6orf130/OARD1 Antibody Picoband® Western blot analysis of C6orf130/OARD1 using anti-C6orf130/OARD1 antibody (A12369). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neoro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C6orf130/OARD1 antigen affinity purified polyclonal antibody (A12369) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for C6orf130/OARD1 at approximately 17 kDa. The expected band size for C6orf130/OARD1 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12369-oard1-primary-antibodies-ihc-testing-2.jpgAnti-C6orf130/OARD1 Antibody Picoband® IHC analysis of C6orf130/OARD1 using anti-C6orf130/OARD1 antibody (A12369). C6orf130/OARD1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C6orf130/OARD1 Antibody (A12369) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12369-oard1-primary-antibodies-if-testing-3.jpgAnti-C6orf130/OARD1 Antibody Picoband® IF analysis of C6orf130/OARD1 using anti-C6orf130/OARD1 antibody (A12369) and anti-Beta Tubulin antibody (M01857-3). C6orf130/OARD1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-C6orf130/OARD1 Antibody (A12369) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12369-oard1-primary-antibodies-fcm-testing-4.jpgAnti-C6orf130/OARD1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-C6orf130/OARD1 antibody (A12369). Overlay histogram showing K562 cells stained with A12369 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C6orf130/OARD1 Antibody (A12369, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294252026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14513-1-qrich1-primary-antibodies-wb-testing-1.jpgAnti-QRICH1 Antibody Picoband® Western blot analysis of QRICH1 using anti-QRICH1 antibody (A14513-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QRICH1 antigen affinity purified polyclonal antibody (A14513-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for QRICH1 at approximately 100 kDa. The expected band size for QRICH1 is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14513-1-qrich1-primary-antibodies-fcm-testing-2.jpgAnti-QRICH1 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-QRICH1 antibody (A14513-1). Overlay histogram showing 293T cells stained with A14513-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-QRICH1 Antibody (A14513-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294262026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32112-1-qtrt2-primary-antibodies-wb-testing-1.jpgAnti-QTRT2 Antibody Picoband® Western blot analysis of QTRT2 using anti-QTRT2 antibody (A32112-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QTRT2 antigen affinity purified polyclonal antibody (A32112-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for QTRT2 at approximately 47 kDa. The expected band size for QTRT2 is at 47 kDa. https://www.bosterbio.com/catalog/product/view/id/1294272026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08360-2-etv2-primary-antibodies-wb-testing-1.jpgAnti-ETV2 Antibody Picoband® Western blot analysis of ETV2 using anti-ETV2 antibody (A08360-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETV2 antigen affinity purified polyclonal antibody (A08360-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ETV2 at approximately 37 kDa. The expected band size for ETV2 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08360-2-etv2-primary-antibodies-fcm-testing-2.jpgAnti-ETV2 Antibody Picoband® Flow Cytometry analysis of RAW264.7 cells using anti-ETV2 antibody (A08360-2). Overlay histogram showing RAW264.7 cells stained with A08360-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ETV2 Antibody (A08360-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294282026-03-17T05:16:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04877-1-scai-primary-antibodies-wb-testing-1.jpgAnti-SCAI Antibody Picoband® Western blot analysis of SCAI using anti-SCAI antibody (A04877-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCAI antigen affinity purified polyclonal antibody (A04877-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SCAI at approximately 66 kDa. The expected band size for SCAI is at 70 kDa. https://www.bosterbio.com/catalog/product/view/id/1294292026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05599-3-odf2-primary-antibodies-wb-testing-1.jpgAnti-ODF2 Antibody Picoband® Western blot analysis of ODF2 using anti-ODF2 antibody (A05599-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF2 antigen affinity purified polyclonal antibody (A05599-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ODF2 at approximately 95 kDa. The expected band size for ODF2 is at 95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05599-3-odf2-primary-antibodies-ihc-testing-2.jpgAnti-ODF2 Antibody Picoband®IHC analysis of ODF2 using anti-ODF2 antibody (A05599-3). ODF2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ODF2 Antibody (A05599-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05599-3-odf2-primary-antibodies-ihc-testing-2_1.jpgAnti-ODF2 Antibody Picoband®IHC analysis of ODF2 using anti-ODF2 antibody (A05599-3). ODF2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ODF2 Antibody (A05599-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05599-3-odf2-primary-antibodies-fcm-testing-3.jpgAnti-ODF2 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-ODF2 antibody (A05599-3). Overlay histogram showing MCF-7 cells stained with A05599-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ODF2 Antibody (A05599-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294302026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07064-2-sgcb-primary-antibodies-wb-testing-1.jpgAnti-Beta Sarcoglycan/SGCB Antibody Picoband® Western blot analysis of Beta Sarcoglycan/SGCB using anti-Beta Sarcoglycan/SGCB antibody (A07064-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta Sarcoglycan/SGCB antigen affinity purified polyclonal antibody (A07064-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Beta Sarcoglycan/SGCB at approximately 45 kDa. The expected band size for Beta Sarcoglycan/SGCB is at 35 kDa. https://www.bosterbio.com/catalog/product/view/id/1294312026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04014-1-ier3-primary-antibodies-wb-testing-1.jpgAnti-IER3 Antibody Picoband® Western blot analysis of IER3 using anti-IER3 antibody (A04014-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IER3 antigen affinity purified polyclonal antibody (A04014-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IER3 at approximately 23 kDa. The expected band size for IER3 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04014-1-ier3-primary-antibodies-ihc-testing-2.jpgAnti-IER3 Antibody Picoband® IHC analysis of IER3 using anti-IER3 antibody (A04014-1). IER3 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IER3 Antibody (A04014-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04014-1-ier3-primary-antibodies-fcm-testing-3.jpgAnti-IER3 Antibody Picoband® Flow Cytometry analysis of Jurkat cells using anti-IER3 antibody (A04014-1). Overlay histogram showing Jurkat cells stained with A04014-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IER3 Antibody (A04014-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294322026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04981-1-sgms1-primary-antibodies-wb-testing-1.jpgAnti-SGMS1 Antibody Picoband® Western blot analysis of SGMS1 using anti-SGMS1 antibody (A04981-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGMS1 antigen affinity purified polyclonal antibody (A04981-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SGMS1 at approximately 55 kDa. The expected band size for SGMS1 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04981-1-k562-cells-primary-antibodies-fcm-testing-2.jpgAnti-SGMS1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-K562 cells antibody (A04981-1). Overlay histogram showing K562 cells stained with A04981-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-K562 cells Antibody (A04981-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294332026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08232-susd2-primary-antibodies-wb-testing-1.jpgAnti-SUSD2 Antibody Picoband® Western blot analysis of SUSD2 using anti-SUSD2 antibody (A08232). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUSD2 antigen affinity purified polyclonal antibody (A08232) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUSD2 at approximately 90 kDa. The expected band size for SUSD2 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08232-susd2-primary-antibodies-fcm-testing-2.jpgAnti-SUSD2 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-SUSD2 antibody (A08232). Overlay histogram showing Caco-2 cells stained with A08232 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SUSD2 Antibody (A08232, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294342026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15590-swt1-primary-antibodies-wb-testing-1.jpgAnti-C1orf26/SWT1 Antibody Picoband® Western blot analysis of C1orf26/SWT1 using anti-C1orf26/SWT1 antibody (A15590). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1orf26/SWT1 antigen affinity purified polyclonal antibody (A15590) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for C1orf26/SWT1 at approximately 103 kDa. The expected band size for C1orf26/SWT1 is at 103 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15590-swt1-primary-antibodies-fcm-testing-2.jpgAnti-C1orf26/SWT1 Antibody Picoband® Flow Cytometry analysis of K562 cells using anti-C1orf26/SWT1 antibody (A15590). Overlay histogram showing K562 cells stained with A15590 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C1orf26/SWT1 Antibody (A15590, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294352026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05119-1-plaat4-primary-antibodies-wb-testing-1.jpgAnti-RARRES3/PLAAT4 Antibody Picoband® Western blot analysis of RARRES3/PLAAT4 using anti-RARRES3/PLAAT4 antibody (A05119-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RARRES3/PLAAT4 antigen affinity purified polyclonal antibody (A05119-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RARRES3/PLAAT4 at approximately 20 kDa. The expected band size for RARRES3/PLAAT4 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05119-1-plaat4-primary-antibodies-ihc-testing-2.jpgAnti-RARRES3/PLAAT4 Antibody Picoband® IHC analysis of RARRES3/PLAAT4 using anti-RARRES3/PLAAT4 antibody (A05119-1). RARRES3/PLAAT4 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RARRES3/PLAAT4 Antibody (A05119-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05119-1-plaat4-primary-antibodies-ihc-testing-3.jpgAnti-RARRES3/PLAAT4 Antibody Picoband® IHC analysis of RARRES3/PLAAT4 using anti-RARRES3/PLAAT4 antibody (A05119-1). RARRES3/PLAAT4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RARRES3/PLAAT4 Antibody (A05119-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05119-1-plaat4-primary-antibodies-ihc-testing-4.jpgAnti-RARRES3/PLAAT4 Antibody Picoband® IHC analysis of RARRES3/PLAAT4 using anti-RARRES3/PLAAT4 antibody (A05119-1). RARRES3/PLAAT4 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RARRES3/PLAAT4 Antibody (A05119-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05119-1-plaat4-primary-antibodies-ihc-testing-5.jpgAnti-RARRES3/PLAAT4 Antibody Picoband® IHC analysis of RARRES3/PLAAT4 using anti-RARRES3/PLAAT4 antibody (A05119-1). RARRES3/PLAAT4 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RARRES3/PLAAT4 Antibody (A05119-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05119-1-plaat4-primary-antibodies-if-testing-6.jpgAnti-RARRES3/PLAAT4 Antibody Picoband® IF analysis of RARRES3/PLAAT4 using anti-RARRES3/PLAAT4 antibody (A05119-1). RARRES3/PLAAT4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RARRES3/PLAAT4 Antibody (A05119-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1294362026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05490-rims1-primary-antibodies-wb-testing-1.jpgAnti-RIM1/RIMS1 Antibody Picoband® Western blot analysis of RIM1/RIMS1 using anti-RIM1/RIMS1 antibody (A05490). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIM1/RIMS1 antigen affinity purified polyclonal antibody (A05490) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RIM1/RIMS1 at approximately 189 kDa. The expected band size for RIM1/RIMS1 is at 189 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05490-rims1-primary-antibodies-ihc-testing-2.jpgAnti-RIM1/RIMS1 Antibody Picoband® IHC analysis of RIM1/RIMS1 using anti-RIM1/RIMS1 antibody (A05490). RIM1/RIMS1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RIM1/RIMS1 Antibody (A05490) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05490-rims1-primary-antibodies-ihc-testing-3.jpgAnti-RIM1/RIMS1 Antibody Picoband® IHC analysis of RIM1/RIMS1 using anti-RIM1/RIMS1 antibody (A05490). RIM1/RIMS1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RIM1/RIMS1 Antibody (A05490) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1294372026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13216-1-prdm6-primary-antibodies-wb-testing-1.jpgAnti-PRDM6 Antibody Picoband® Western blot analysis of PRDM6 using anti-PRDM6 antibody (A13216-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM6 antigen affinity purified polyclonal antibody (A13216-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRDM6 at approximately 61 kDa. The expected band size for PRDM6 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13216-1-prdm6-primary-antibodies-if-testing-2.jpgAnti-PRDM6 Antibody Picoband® IF analysis of PRDM6 using anti-PRDM6 antibody (A13216-1) and anti-Beta Tubulin antibody (M01857-3). PRDM6 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRDM6 Antibody (A13216-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13216-1-prdm6-primary-antibodies-fcm-testing-3.jpgAnti-PRDM6 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-PRDM6 antibody (A13216-1). Overlay histogram showing MCF-7 cells stained with A13216-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM6 Antibody (A13216-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294382026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12220-1-psmd5-primary-antibodies-wb-testing-1.jpgAnti-PSMD5 Antibody Picoband® Western blot analysis of PSMD5 using anti-PSMD5 antibody (A12220-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD5 antigen affinity purified polyclonal antibody (A12220-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMD5 at approximately 56 kDa. The expected band size for PSMD5 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12220-1-psmd5-primary-antibodies-ihc-testing-2.jpgAnti-PSMD5 Antibody Picoband® IHC analysis of PSMD5 using anti-PSMD5 antibody (A12220-1). PSMD5 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD5 Antibody (A12220-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12220-1-psmd5-primary-antibodies-if-testing-3.jpgAnti-PSMD5 Antibody Picoband® IF analysis of PSMD5 using anti-PSMD5 antibody (A12220-1) and anti-Beta Tubulin antibody (M01857-3). PSMD5 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMD5 Antibody (A12220-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12220-1-psmd5-primary-antibodies-fcm-testing-4.jpgAnti-PSMD5 Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-PSMD5 antibody (A12220-1). Overlay histogram showing 293T cells stained with A12220-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD5 Antibody (A12220-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294392026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03169-2-psmd10-primary-antibodies-wb-testing-1.jpgAnti-Gankyrin/PSMD10 Antibody Picoband® Western blot analysis of Gankyrin/PSMD10 using anti-Gankyrin/PSMD10 antibody (A03169-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gankyrin/PSMD10 antigen affinity purified polyclonal antibody (A03169-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Gankyrin/PSMD10 at approximately 24 kDa. The expected band size for Gankyrin/PSMD10 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03169-2-psmd10-primary-antibodies-fcm-testing-2.jpgAnti-Gankyrin/PSMD10 Antibody Picoband® Flow Cytometry analysis of MCF-7 cells using anti-Gankyrin/PSMD10 antibody (A03169-2). Overlay histogram showing MCF-7 cells stained with A03169-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gankyrin/PSMD10 Antibody (A03169-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294402026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10147-1-psme4-primary-antibodies-wb-testing-1.jpgAnti-PSME4 Antibody Picoband® Western blot analysis of PSME4 using anti-PSME4 antibody (A10147-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSME4 antigen affinity purified polyclonal antibody (A10147-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSME4 at approximately 211 kDa. The expected band size for PSME4 is at 211 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10147-1-psme4-primary-antibodies-if-testing-2.jpgAnti-PSME4 Antibody Picoband® IF analysis of PSME4 using anti-PSME4 antibody (A10147-1) and anti-Beta Tubulin antibody (M01857-3). PSME4 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSME4 Antibody (A10147-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10147-1-psme4-primary-antibodies-fcm-testing-3.jpgAnti-PSME4 Antibody Picoband® Flow Cytometry analysis of HepG2 cells using anti-PSME4 antibody (A10147-1). Overlay histogram showing HepG2 cells stained with A10147-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSME4 Antibody (A10147-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294412026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11257-4-pum3-primary-antibodies-wb-testing-1.jpgAnti-PUF-A/KIAA0020/PUM3 Antibody Picoband® Western blot analysis of PUF-A/KIAA0020/PUM3 using anti-PUF-A/KIAA0020/PUM3 antibody (A11257-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUF-A/KIAA0020/PUM3 antigen affinity purified polyclonal antibody (A11257-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PUF-A/KIAA0020/PUM3 at approximately 70 kDa. The expected band size for PUF-A/KIAA0020/PUM3 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11257-4-pum3-primary-antibodies-fcm-testing-2.jpgAnti-PUF-A/KIAA0020/PUM3 Antibody Picoband® Flow Cytometry analysis of A549 cells using anti-PUF-A/KIAA0020/PUM3 antibody (A11257-4). Overlay histogram showing A549 cells stained with A11257-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PUF-A/KIAA0020/PUM3 Antibody (A11257-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294422026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05047-2-noxo1-primary-antibodies-wb-testing-1.jpgAnti-NOXO1 Antibody Picoband® Western blot analysis of NOXO1 using anti-NOXO1 antibody (A05047-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse small intestine tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOXO1 antigen affinity purified polyclonal antibody (A05047-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NOXO1 at approximately 41 kDa. The expected band size for NOXO1 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05047-2-noxo1-primary-antibodies-ihc-testing-2.jpgAnti-NOXO1 Antibody Picoband® IHC analysis of NOXO1 using anti-NOXO1 antibody (A05047-2). NOXO1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOXO1 Antibody (A05047-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05047-2-noxo1-primary-antibodies-ihc-testing-3.jpgAnti-NOXO1 Antibody Picoband® IHC analysis of NOXO1 using anti-NOXO1 antibody (A05047-2). NOXO1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOXO1 Antibody (A05047-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1294432026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05744-2-puf60-primary-antibodies-wb-testing-1.jpgAnti-PUF60 Antibody Picoband® Western blot analysis of PUF60 using anti-PUF60 antibody (A05744-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUF60 antigen affinity purified polyclonal antibody (A05744-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PUF60 at approximately 60 kDa. The expected band size for PUF60 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05744-2-puf60-primary-antibodies-ihc-testing-3.jpgAnti-PUF60 Antibody Picoband® IHC analysis of PUF60 using anti-PUF60 antibody (A05744-2). PUF60 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PUF60 Antibody (A05744-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05744-2-puf60-primary-antibodies-ihc-testing-2.jpgAnti-PUF60 Antibody Picoband® IHC analysis of PUF60 using anti-PUF60 antibody (A05744-2). PUF60 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PUF60 Antibody (A05744-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05744-2-puf60-primary-antibodies-ip-testing-1.jpgAnti-PUF60 Antibody Picoband®Immunoprecipitating (IP) PUF60 in MCF-7 whole cell lysate. Western blot analysis of PUF60 using anti-PUF60 antibody (A05744-2); Lane 1: MCF-7 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-PUF60 antibody in MCF-7 whole cell lysate; Lane 3: anti-PUF60 antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PUF60 antigen affinity purified polyclonal antibody (A05744-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PUF60 at approximately 60 kDa. The expected band size for PUF60 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05744-2-puf60-primary-antibodies-fcm-testing-4.jpgAnti-PUF60 Antibody Picoband® Flow Cytometry analysis of Caco-2 cells using anti-PUF60 antibody (A05744-2). Overlay histogram showing Caco-2 cells stained with A05744-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PUF60 Antibody (A05744-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294442026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13474-1-naa80-primary-antibodies-wb-testing-1.jpgAnti-NAT6/FUS2/NAA80 Antibody Picoband® Western blot analysis of NAT6/FUS2/NAA80 using anti-NAT6/FUS2/NAA80 antibody (A13474-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAT6/FUS2/NAA80 antigen affinity purified polyclonal antibody (A13474-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NAT6/FUS2/NAA80 at approximately 35 kDa. The expected band size for NAT6/FUS2/NAA80 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13474-1-naa80-primary-antibodies-fcm-testing-2.jpgAnti-NAT6/FUS2/NAA80 Antibody Picoband® Flow Cytometry analysis of THP-1 cells using anti-NAT6/FUS2/NAA80 antibody (A13474-1). Overlay histogram showing THP-1 cells stained with A13474-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NAT6/FUS2/NAA80 Antibody (A13474-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294452026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05458-2-ing3-primary-antibodies-wb-testing-1.jpgAnti-ING3 Antibody Picoband® Western blot analysis of ING3 using anti-ING3 antibody (A05458-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ING3 antigen affinity purified polyclonal antibody (A05458-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ING3 at approximately 47 kDa. The expected band size for ING3 is at 47 kDa. https://www.bosterbio.com/catalog/product/view/id/1294462026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05929-1-hbs1l-primary-antibodies-wb-testing-1.jpgAnti-HBS1L Antibody Picoband® Western blot analysis of HBS1L using anti-HBS1L antibody (A05929-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBS1L antigen affinity purified polyclonal antibody (A05929-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HBS1L at approximately 75 kDa. The expected band size for HBS1L is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05929-1-hbs1l-primary-antibodies-fcm-testing-2.jpgAnti-HBS1L Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-HBS1L antibody (A05929-1). Overlay histogram showing HEL cells stained with A05929-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HBS1L Antibody (A05929-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294472026-03-17T05:16:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09183-1-htr3e-primary-antibodies-wb-testing-1.jpgAnti-HTR3E Antibody Picoband® Western blot analysis of HTR3E using anti-HTR3E antibody (A09183-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTR3E antigen affinity purified polyclonal antibody (A09183-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HTR3E at approximately 51 kDa. The expected band size for HTR3E is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09183-1-htr3e-primary-antibodies-ihc-testing-2.jpgAnti-HTR3E Antibody Picoband® IHC analysis of HTR3E using anti-HTR3E antibody (A09183-1). HTR3E was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HTR3E Antibody (A09183-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1294482026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06496-3-hand1-primary-antibodies-wb-testing-1.jpgAnti-HAND1 Antibody Picoband® Western blot analysis of HAND1 using anti-HAND1 antibody (A06496-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAND1 antigen affinity purified polyclonal antibody (A06496-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HAND1 at approximately 24 kDa. The expected band size for HAND1 is at 24 kDa. https://www.bosterbio.com/catalog/product/view/id/1294492026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09133-2-hoxb6-primary-antibodies-wb-testing-1.jpgAnti-HOXB6 Antibody Picoband® Western blot analysis of HOXB6 using anti-HOXB6 antibody (A09133-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXB6 antigen affinity purified polyclonal antibody (A09133-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HOXB6 at approximately 25 kDa. The expected band size for HOXB6 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09133-2-hoxb6-primary-antibodies-fcm-testing-2.jpgAnti-HOXB6 Antibody Picoband® Flow Cytometry analysis of Hela cells using anti-HOXB6 antibody (A09133-2). Overlay histogram showing Hela cells stained with A09133-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HOXB6 Antibody (A09133-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294502026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01066-1-ptk2b-primary-antibodies-wb-testing-1_1.jpgAnti-PYK2/PTK2B Antibody Picoband® Western blot analysis of PYK2/PTK2B using anti-PYK2/PTK2B antibody (A01066-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYK2/PTK2B antigen affinity purified polyclonal antibody (A01066-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PYK2/PTK2B at approximately 116 kDa. The expected band size for PYK2/PTK2B is at 116 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01066-1-ptk2b-primary-antibodies-ihc-testing-2.jpgAnti-PYK2/PTK2B Antibody Picoband® IHC analysis of PYK2/PTK2B using anti-PYK2/PTK2B antibody (A01066-1). PYK2/PTK2B was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYK2/PTK2B Antibody (A01066-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01066-1-ptk2b-primary-antibodies-if-testing-3.jpgAnti-PYK2/PTK2B Antibody Picoband® IF analysis of PYK2/PTK2B using anti-PYK2/PTK2B antibody (A01066-1) and anti-Beta Tubulin antibody (M01857-3). PYK2/PTK2B was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PYK2/PTK2B Antibody (A01066-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01066-1-ptk2b-primary-antibodies-fcm-testing-4.jpgAnti-PYK2/PTK2B Antibody Picoband® Flow Cytometry analysis of A431 cells using anti-PYK2/PTK2B antibody (A01066-1). Overlay histogram showing A431 cells stained with A01066-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYK2/PTK2B Antibody (A01066-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294512026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00808-1-il21r-primary-antibodies-wb-testing-1.jpgAnti-IL21R Antibody Picoband® Western blot analysis of IL21R using anti-IL21R antibody (A00808-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL21R antigen affinity purified polyclonal antibody (A00808-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL21R at approximately 70 kDa. The expected band size for IL21R is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00808-1-il21r-primary-antibodies-ihc-testing-2.jpgAnti-IL21R Antibody Picoband® IHC analysis of IL21R using anti-IL21R antibody (A00808-1). IL21R was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL21R Antibody (A00808-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00808-1-il21r-primary-antibodies-ihc-testing-3.jpgAnti-IL21R Antibody Picoband® IHC analysis of IL21R using anti-IL21R antibody (A00808-1). IL21R was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL21R Antibody (A00808-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00808-1-il21r-primary-antibodies-fcm-testing-4.jpgAnti-IL21R Antibody Picoband® Flow Cytometry analysis of HEL cells using anti-IL21R antibody (A00808-1). Overlay histogram showing HEL cells stained with A00808-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IL21R Antibody (A00808-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294522026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04133-4-marcks-primary-antibodies-wb-testing-1.jpgAnti-MARCKS Antibody Picoband® Western blot analysis of MARCKS using anti-MARCKS antibody (A04133-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARCKS antigen affinity purified polyclonal antibody (A04133-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MARCKS at approximately 80 kDa. The expected band size for MARCKS is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04133-4-marcks-primary-antibodies-ihc-testing-2.jpgAnti-MARCKS Antibody Picoband® IHC analysis of MARCKS using anti-MARCKS antibody (A04133-4). MARCKS was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MARCKS Antibody (A04133-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04133-4-marcks-primary-antibodies-ihc-testing-3.jpgAnti-MARCKS Antibody Picoband® IHC analysis of MARCKS using anti-MARCKS antibody (A04133-4). MARCKS was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MARCKS Antibody (A04133-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04133-4-marcks-primary-antibodies-ihc-testing-4.jpgAnti-MARCKS Antibody Picoband® IHC analysis of MARCKS using anti-MARCKS antibody (A04133-4). MARCKS was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MARCKS Antibody (A04133-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04133-4-marcks-primary-antibodies-fcm-testing-5.jpgAnti-MARCKS Antibody Picoband® Flow Cytometry analysis of 293T cells using anti-MARCKS antibody (A04133-4). Overlay histogram showing 293T cells stained with A04133-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MARCKS Antibody (A04133-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1294532026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32233-marcol-primary-antibodies-wb-testing-1.jpgAnti-MARCOL Antibody Picoband® Western blot analysis of MARCOL using anti-MARCOL antibody (A32233). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates, Lane 5: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MARCOL antigen affinity purified polyclonal antibody (A32233) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MARCOL at approximately 30 kDa. The expected band size for MARCOL is at 30 kDa. https://www.bosterbio.com/catalog/product/view/id/1294542026-03-24T05:36:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07653-2-gimap5-primary-antibodies-wb-testing-1_1.jpgAnti-GIMAP5 Antibody Picoband®Western blot analysis of GIMAP5 using anti-GIMAP5 antibody (A07653-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIMAP5 antigen affinity purified polyclonal antibody (A07653-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GIMAP5 at approximately 35 kDa. The expected band size for GIMAP5 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07653-2-gimap5-primary-antibodies-if-testing-1.jpgAnti-GIMAP5 Antibody Picoband®IF analysis of GIMAP5 using anti-GIMAP5 antibody (A07653-2). GIMAP5 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GIMAP5 Antibody (A07653-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07653-2-gimap5-primary-antibodies-ip-testing-1.jpgAnti-GIMAP5 Antibody Picoband®Immunoprecipitating (IP) GIMAP5 in Jurkat whole cell lysate. Western blot analysis of GIMAP5 using anti-GIMAP5 antibody (A07653-2); Lane 1: Jurkat whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-GIMAP5 antibody in Jurkat whole cell lysate; Lane 3: anti-GIMAP5 antibody (2μg) + Jurkat whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GIMAP5 antigen affinity purified polyclonal antibody (A07653-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for GIMAP5 at approximately 35 kDa. The expected band size for GIMAP5 is at 35 kDa. https://www.bosterbio.com/catalog/product/view/id/1294552026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08286-2-gzmm-primary-antibodies-ihc-testing-1.jpgAnti-Granzyme M/GZMM Antibody IHC analysis of Granzyme M/GZMM using anti-Granzyme M/GZMM antibody (A08286-2). Granzyme M/GZMM was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Granzyme M/GZMM Antibody (A08286-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08286-2-gzmm-primary-antibodies-ihc-testing-2.jpgAnti-Granzyme M/GZMM Antibody IHC analysis of Granzyme M/GZMM using anti-Granzyme M/GZMM antibody (A08286-2). Granzyme M/GZMM was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Granzyme M/GZMM Antibody (A08286-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1294562026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17177-spink4-primary-antibodies-ihc-testing-1.jpgAnti-SPINK4 Antibody IHC analysis of SPINK4 using anti-SPINK4 antibody (A17177). SPINK4 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPINK4 Antibody (A17177) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/human-cxcl4-pf4-elisa-kit-ez-settm-diy-antibody-pairs-ez726-oster.html2026-03-10T04:40:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0726_1.pngHuman CXCL4/PF4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)Human CXCL4/PF4 EZ-Set ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-wolbachia-antibody-dz33973-1-boster.html2026-04-03T05:00:57+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ush2a-antibody.html2026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz01481-1_1_1.jpgAnti-Zebrafish Ush2a Antibody IF analysis of Ush2a using anti-Ush2a antibody (DZ01481). Ush2a was detected in cryosections of wildtype zebrafish retina tissue without prior fixation. The tissue section was blocked with Blocking buffer (10% NGS, 2% BSA, 0.1% Tween in PBS) for 30 minutes. The tissue section was then incubated with rabbit anti-Ush2a Antibody (DZ01481) in blocking solution at 1:200 dilution overnight at 4°C. Alexa Fluor® 568 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody a and incubated for 1 hour at room temperature in blocking solution. Post immuno-fixation with 4% PFA in PBS for 10 minutes. Mount in Prolong gold.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz01481-gr5_lrg.jpgAnti-Zebrafish Ush2a AntibodyAnalysis of usherin and Whrnb expression in retinal sections of wild-type, adgrv1rmc22, and adgrv1Δexon40-42 zebrafish. Retinal cryosections of wild-type, mutant adgrv1rmc22, and adgrv1Δexon40-42 zebrafish larvae (5 dpf) labeled with antibodies directed against Whrnb (red) (A) or usherin (red) (B) and centrin (green). Nuclei are counterstained with DAPI (blue). (A) Both in wild-type and in adgrv1Δexon40-42 zebrafish larvae, Whrnb was present at the photoreceptor periciliary region in close proximity to centrin. Quantification revealed that the Whrnb signal intensity in adgrv1Δexon40-42 retinal sections is similar to that in wild-type sections, whereas a significant reduction of Whrnb signal was observed in retinae of adgrv1rmc22 larvae. Magnified images are shown at the right side. Bar graphs represent the mean fluorescence intensity of anti-Whrnb staining of all photoreceptors of a single, central section of one larval zebrafish eye (n = 14 eyes). (B) Both in wild-type and adgrv1Δexon40-42 zebrafish larvae, usherin was present at the photoreceptor periciliary region in close proximity to centrin. Quantification reveals that the usherin signal intensity in retinal sections of adgrv1Δexon40-42 larvae is similar to that in sections of wild types, whereas a significant reduction of usherin signal was observed in retinae of adgrv1rmc22 larvae. Bar graphs represent the mean fluorescence intensity of anti-usherin staining of all photoreceptors of a single, central section of one larval zebrafish eye, with mean ± SD (n = 14 eyes). Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test (∗∗p < 0.01; ∗∗∗∗p < 0.0001). Scale bars, 10 μm. Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(25)00256-2'>41036464</a> https://www.bosterbio.com/products/nitrocellulose-membrane-for-western-blot-transfer-pore-0-25-micro-m-9-cm-x-10cm-ar0135-02-boster.html2026-03-31T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar0135-02_1.jpgNitrocellulose Membrane For Western Blot Transfer, Pore 0.25 µm, 9 cm x 10cm https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cdk5-antibody-azq9de44-boster.html2026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9de44-cdk5-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CDK5 Antibody Picoband® Western blot analysis of CDK5 using anti-CDK5 antibody (AZQ9DE44). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK5 antigen affinity purified polyclonal antibody (AZQ9DE44) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CDK5 at approximately 33 kDa. The expected band size for CDK5 is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ckba-b-antibody-aza0a8m6z2b4-boster.html2026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6z2b4-ckba-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CKBa/b Antibody Picoband® Western blot analysis of CKBa/b using anti-CKBa/b antibody (AZA0A8M6Z2B4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CKBa/b antigen affinity purified polyclonal antibody (AZA0A8M6Z2B4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CKBa/b at approximately 40 kDa. The expected band size for CKBa/b is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6z2b4-ckba-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CKBa/b Antibody Picoband® IHC analysis of CKBa/b using anti-CKBa/b antibody (AZA0A8M6Z2B4). CKBa/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CKBa/b Antibody (AZA0A8M6Z2B4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6z2b4-ckba-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish CKBa/b Antibody Picoband® IHC analysis of CKBa/b using anti-CKBa/b antibody (AZA0A8M6Z2B4). CKBa/b was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CKBa/b Antibody (AZA0A8M6Z2B4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ckma-b-antibody-aza2bha3-boster.html2026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2bha3-ckma-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CKMa/b Antibody Picoband® Western blot analysis of CKMa/b using anti-CKMa/b antibody (AZA2BHA3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CKMa/b antigen affinity purified polyclonal antibody (AZA2BHA3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CKMa/b at approximately 40 kDa. The expected band size for CKMa/b is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2bha3-ckma-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CKMa/b Antibody Picoband® IHC analysis of CKMa/b using anti-CKMa/b antibody (AZA2BHA3). CKMa/b was detected in a paraffin-embedded section of zebrafish spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CKMa/b Antibody (AZA2BHA3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2bha3-ckma-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish CKMa/b Antibody Picoband® IHC analysis of CKMa/b using anti-CKMa/b antibody (AZA2BHA3). CKMa/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CKMa/b Antibody (AZA2BHA3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2bha3-ckma-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish CKMa/b Antibody Picoband® IHC analysis of CKMa/b using anti-CKMa/b antibody (AZA2BHA3). CKMa/b was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CKMa/b Antibody (AZA2BHA3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-clpxa-b-antibody-aza0a8m9qbu1-boster.html2026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qbu1-clpxa-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CLPXa/b Antibody Picoband® Western blot analysis of CLPXa/b using anti-CLPXa/b antibody (AZA0A8M9QBU1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLPXa/b antigen affinity purified polyclonal antibody (AZA0A8M9QBU1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CLPXa/b at approximately 69 kDa. The expected band size for CLPXa/b is at 69 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cope-antibody-azq5u3e8-boster.html2026-03-17T05:16:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u3e8-cope-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish COPE Antibody IHC analysis of COPE using anti-COPE antibody (AZQ5U3E8). COPE was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COPE Antibody (AZQ5U3E8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u3e8-cope-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish COPE Antibody IHC analysis of COPE using anti-COPE antibody (AZQ5U3E8). COPE was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COPE Antibody (AZQ5U3E8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u3e8-cope-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish COPE Antibody IHC analysis of COPE using anti-COPE antibody (AZQ5U3E8). COPE was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COPE Antibody (AZQ5U3E8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-jab1-cops5-antibody-azq6pc30-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pc30-jab1-cops5-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish JAB1/COPS5 Antibody Picoband® Western blot analysis of JAB1/COPS5 using anti-JAB1/COPS5 antibody (AZQ6PC30). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAB1/COPS5 antigen affinity purified polyclonal antibody (AZQ6PC30) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for JAB1/COPS5 at approximately 38 kDa. The expected band size for JAB1/COPS5 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pc30-jab1-cops5-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish JAB1/COPS5 Antibody Picoband® IHC analysis of JAB1/COPS5 using anti-JAB1/COPS5 antibody (AZQ6PC30). JAB1/COPS5 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JAB1/COPS5 Antibody (AZQ6PC30) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pc30-jab1-cops5-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish JAB1/COPS5 Antibody Picoband® IHC analysis of JAB1/COPS5 using anti-JAB1/COPS5 antibody (AZQ6PC30). JAB1/COPS5 was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JAB1/COPS5 Antibody (AZQ6PC30) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pc30-jab1-cops5-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish JAB1/COPS5 Antibody Picoband® IHC analysis of JAB1/COPS5 using anti-JAB1/COPS5 antibody (AZQ6PC30). JAB1/COPS5 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JAB1/COPS5 Antibody (AZQ6PC30) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pc30-jab1-cops5-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish JAB1/COPS5 Antibody Picoband® IHC analysis of JAB1/COPS5 using anti-JAB1/COPS5 antibody (AZQ6PC30). JAB1/COPS5 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JAB1/COPS5 Antibody (AZQ6PC30) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pc30-jab1-cops5-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish JAB1/COPS5 Antibody Picoband® IHC analysis of JAB1/COPS5 using anti-JAB1/COPS5 antibody (AZQ6PC30). JAB1/COPS5 was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JAB1/COPS5 Antibody (AZQ6PC30) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-crkl-antibody-azq6ph06-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph06-crkl-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CRKL Antibody Picoband® Western blot analysis of CRKL using anti-CRKL antibody (AZQ6PH06). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRKL antigen affinity purified polyclonal antibody (AZQ6PH06) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CRKL at approximately 37 kDa. The expected band size for CRKL is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph06-crkl-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CRKL Antibody Picoband® IHC analysis of CRKL using anti-CRKL antibody (AZQ6PH06). CRKL was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CRKL Antibody (AZQ6PH06) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph06-crkl-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish CRKL Antibody Picoband® IHC analysis of CRKL using anti-CRKL antibody (AZQ6PH06). CRKL was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CRKL Antibody (AZQ6PH06) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph06-crkl-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish CRKL Antibody Picoband® IHC analysis of CRKL using anti-CRKL antibody (AZQ6PH06). CRKL was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CRKL Antibody (AZQ6PH06) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph06-crkl-primary-antibodies-if-testing-5.jpgAnti-Zebrafish CRKL Antibody Picoband® IF analysis of CRKL using anti-CRKL antibody (AZQ6PH06). CRKL was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CRKL Antibody (AZQ6PH06) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-csnk2b-antibody-azq91398-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq91398-csnk2b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CSNK2B Antibody Picoband® Western blot analysis of CSNK2B using anti-CSNK2B antibody (AZQ91398). Electrophoresis was performed on a 12% SDS-PAGE SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSNK2B antigen affinity purified polyclonal antibody (AZQ91398) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CSNK2B at approximately 25 kDa. The expected band size for CSNK2B is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq91398-csnk2b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CSNK2B Antibody Picoband® IHC analysis of CSNK2B using anti-CSNK2B antibody (AZQ91398). CSNK2B was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2B Antibody (AZQ91398) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq91398-csnk2b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish CSNK2B Antibody Picoband® IHC analysis of CSNK2B using anti-CSNK2B antibody (AZQ91398). CSNK2B was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2B Antibody (AZQ91398) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dazap1-antibody-azq29r83-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq29r83-dazap1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DAZAP1 Antibody Picoband® Western blot analysis of DAZAP1 using anti-DAZAP1 antibody (AZQ29R83). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAZAP1 antigen affinity purified polyclonal antibody (AZQ29R83) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DAZAP1 at approximately 39 kDa. The expected band size for DAZAP1 is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ddx6-antibody-aze7fd91-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7fd91-ddx6-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DDX6 Antibody Picoband® Western blot analysis of DDX6 using anti-DDX6 antibody (AZE7FD91). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX6 antigen affinity purified polyclonal antibody (AZE7FD91) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX6 at approximately 54 kDa. The expected band size for DDX6 is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dhx9-antibody-aza0a8m3aqb4-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3aqb4-dhx9-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish DHX9 Antibody IHC analysis of DHX9 using anti-DHX9 antibody (AZA0A8M3AQB4). DHX9 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX9 Antibody (AZA0A8M3AQB4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3aqb4-dhx9-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish DHX9 Antibody IHC analysis of DHX9 using anti-DHX9 antibody (AZA0A8M3AQB4). DHX9 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX9 Antibody (AZA0A8M3AQB4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3aqb4-dhx9-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish DHX9 Antibody IHC analysis of DHX9 using anti-DHX9 antibody (AZA0A8M3AQB4). DHX9 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX9 Antibody (AZA0A8M3AQB4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3aqb4-dhx9-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish DHX9 Antibody IHC analysis of DHX9 using anti-DHX9 antibody (AZA0A8M3AQB4). DHX9 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX9 Antibody (AZA0A8M3AQB4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3aqb4-dhx9-primary-antibodies-if-testing-5.jpgAnti-Zebrafish DHX9 Antibody IF analysis of DHX9 using anti-DHX9 antibody (AZA0A8M3AQB4). DHX9 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DHX9 Antibody (AZA0A8M3AQB4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dicer1-antibody-azq6tv19-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tv19-dicer1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DICER1 Antibody Picoband® Western blot analysis of DICER1 using anti-DICER1 antibody (AZQ6TV19). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DICER1 antigen affinity purified polyclonal antibody (AZQ6TV19) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DICER1 at approximately 250 kDa. The expected band size for DICER1 is at 219 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dlst-antibody-azq7zvl3-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvl3-dlst-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DLST Antibody Picoband® Western blot analysis of DLST using anti-DLST antibody (AZQ7ZVL3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLST antigen affinity purified polyclonal antibody (AZQ7ZVL3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DLST at approximately 53 kDa. The expected band size for DLST is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvl3-dlst-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish DLST Antibody Picoband® IHC analysis of DLST using anti-DLST antibody (AZQ7ZVL3). DLST was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DLST Antibody (AZQ7ZVL3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvl3-dlst-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish DLST Antibody Picoband® IHC analysis of DLST using anti-DLST antibody (AZQ7ZVL3). DLST was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DLST Antibody (AZQ7ZVL3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvl3-dlst-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish DLST Antibody Picoband® IHC analysis of DLST using anti-DLST antibody (AZQ7ZVL3). DLST was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DLST Antibody (AZQ7ZVL3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvl3-dlst-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish DLST Antibody Picoband® IHC analysis of DLST using anti-DLST antibody (AZQ7ZVL3). DLST was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DLST Antibody (AZQ7ZVL3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvl3-dlst-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish DLST Antibody Picoband® IHC analysis of DLST using anti-DLST antibody (AZQ7ZVL3). DLST was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DLST Antibody (AZQ7ZVL3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dnaja2a-b-antibody-azq7zup5-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zup5-dnaja2a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DNAJA2a/b Antibody Picoband® Western blot analysis of DNAJA2a/b using anti-DNAJA2a/b antibody (AZQ7ZUP5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJA2a/b antigen affinity purified polyclonal antibody (AZQ7ZUP5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNAJA2a/b at approximately 46 kDa. The expected band size for DNAJA2a/b is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zup5-dnaja2a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish DNAJA2a/b Antibody Picoband® IHC analysis of DNAJA2a/b using anti-DNAJA2a/b antibody (AZQ7ZUP5). DNAJA2a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAJA2a/b Antibody (AZQ7ZUP5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zup5-dnaja2a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish DNAJA2a/b Antibody Picoband® IHC analysis of DNAJA2a/b using anti-DNAJA2a/b antibody (AZQ7ZUP5). DNAJA2a/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAJA2a/b Antibody (AZQ7ZUP5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zup5-dnaja2a-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish DNAJA2a/b Antibody Picoband® IHC analysis of DNAJA2a/b using anti-DNAJA2a/b antibody (AZQ7ZUP5). DNAJA2a/b was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAJA2a/b Antibody (AZQ7ZUP5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zup5-dnaja2a-b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish DNAJA2a/b Antibody Picoband® IHC analysis of DNAJA2a/b using anti-DNAJA2a/b antibody (AZQ7ZUP5). DNAJA2a/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAJA2a/b Antibody (AZQ7ZUP5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-eed-antibody-azq566t0-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq566t0-eed-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish EED Antibody Picoband® Western blot analysis of EED using anti-EED antibody (AZQ566T0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EED antigen affinity purified polyclonal antibody (AZQ566T0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EED at approximately 45 kDa. The expected band size for EED is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-eif3a-antibody-azq6pcr7-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pcr7-eif3a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish EIF3A Antibody Picoband® Western blot analysis of EIF3A using anti-EIF3A antibody (AZQ6PCR7). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3A antigen affinity purified polyclonal antibody (AZQ6PCR7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EIF3A at approximately 220 kDa. The expected band size for EIF3A is at 167 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pcr7-eif3a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish EIF3A Antibody Picoband® IHC analysis of EIF3A using anti-EIF3A antibody (AZQ6PCR7). EIF3A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3A Antibody (AZQ6PCR7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pcr7-eif3a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish EIF3A Antibody Picoband® IHC analysis of EIF3A using anti-EIF3A antibody (AZQ6PCR7). EIF3A was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3A Antibody (AZQ6PCR7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pcr7-eif3a-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish EIF3A Antibody Picoband® IHC analysis of EIF3A using anti-EIF3A antibody (AZQ6PCR7). EIF3A was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3A Antibody (AZQ6PCR7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pcr7-eif3a-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish EIF3A Antibody Picoband® IHC analysis of EIF3A using anti-EIF3A antibody (AZQ6PCR7). EIF3A was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3A Antibody (AZQ6PCR7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-eif4a1a-b-2-antibody-azq802c9-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq802c9-eif4a1a-b-2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish EIF4A1a/b/2 Antibody Picoband® Western blot analysis of EIF4A1a/b/2 using anti-EIF4A1a/b/2 antibody (AZQ802C9). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4A1a/b/2 antigen affinity purified polyclonal antibody (AZQ802C9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EIF4A1a/b/2 at approximately 46 kDa. The expected band size for EIF4A1a/b/2 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq802c9-eif4a1a-b-2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish EIF4A1a/b/2 Antibody Picoband® IHC analysis of EIF4A1a/b/2 using anti-EIF4A1a/b/2 antibody (AZQ802C9). EIF4A1a/b/2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF4A1a/b/2 Antibody (AZQ802C9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq802c9-eif4a1a-b-2-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish EIF4A1a/b/2 Antibody Picoband® IHC analysis of EIF4A1a/b/2 using anti-EIF4A1a/b/2 antibody (AZQ802C9). EIF4A1a/b/2 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF4A1a/b/2 Antibody (AZQ802C9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-eif4a2-antibody-azf1r166-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r166-eif4a2-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish EIF4A2 Antibody Picoband® IHC analysis of EIF4A2 using anti-EIF4A2 antibody (AZF1R166). EIF4A2 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF4A2 Antibody (AZF1R166) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r166-eif4a2-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish EIF4A2 Antibody Picoband® IHC analysis of EIF4A2 using anti-EIF4A2 antibody (AZF1R166). EIF4A2 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF4A2 Antibody (AZF1R166) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r166-eif4a2-primary-antibodies-wb-testing-2.jpgAnti-Zebrafish EIF4A2 Antibody Picoband® Western blot analysis of EIF4A2 using anti-EIF4A2 antibody (AZF1R166). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4A2 antigen affinity purified polyclonal antibody (AZF1R166) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EIF4A2 at approximately 46 kDa. The expected band size for EIF4A2 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r166-eif4a2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish EIF4A2 Antibody Picoband®Western blot analysis of EIF4A2 using anti-EIF4A2 antibody (AZF1R166). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4A2 antigen affinity purified polyclonal antibody (AZF1R166) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. The exposure time was 30 seconds. The expected band size for EIF4A2 is at ~46 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r166-eif4a2-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish EIF4A2 Antibody Picoband® IHC analysis of EIF4A2 using anti-EIF4A2 antibody (AZF1R166). EIF4A2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF4A2 Antibody (AZF1R166) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r166-eif4a2-primary-antibodies-if-testing-1.jpgAnti-Zebrafish EIF4A2 Antibody Picoband® IF analysis of EIF4A2 using anti-EIF4A2 antibody (AZF1R166). EIF4A2 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EIF4A2 Antibody (AZF1R166) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-eno1a-antibody-aza0a2r8q1x2-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8q1x2-eno1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ENO1a Antibody Picoband® Western blot analysis of ENO1a using anti-ENO1a antibody (AZA0A2R8Q1X2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO1a antigen affinity purified polyclonal antibody (AZA0A2R8Q1X2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ENO1a at approximately 48 kDa. The expected band size for ENO1a is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8q1x2-eno1a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ENO1a Antibody Picoband® IHC analysis of ENO1a using anti-ENO1a antibody (AZA0A2R8Q1X2). ENO1a was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENO1a Antibody (AZA0A2R8Q1X2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-eno1a-b-antibody-azq6pc89-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pc89-eno1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ENO1a/b Antibody Picoband® Western blot analysis of ENO1a/b using anti-ENO1a/b antibody (AZQ6PC89). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO1a/b antigen affinity purified polyclonal antibody (AZQ6PC89) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ENO1a/b at approximately 48 kDa. The expected band size for ENO1a/b is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pc89-eno1a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ENO1a/b Antibody Picoband® IHC analysis of ENO1a/b using anti-ENO1a/b antibody (AZQ6PC89). ENO1a/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENO1a/b Antibody (AZQ6PC89) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-etf1a-b-antibody-aza8kb65-boster.html2026-03-17T05:16:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8kb65-etf1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ETF1a/b Antibody Picoband® Western blot analysis of ETF1a/b using anti-ETF1a/b antibody (AZA8KB65). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETF1a/b antigen affinity purified polyclonal antibody (AZA8KB65) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ETF1a/b at approximately 55 kDa. The expected band size for ETF1a/b is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8kb65-etf1a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ETF1a/b Antibody Picoband® IHC analysis of ETF1a/b using anti-ETF1a/b antibody (AZA8KB65). ETF1a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ETF1a/b Antibody (AZA8KB65) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8kb65-etf1a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish ETF1a/b Antibody Picoband® IHC analysis of ETF1a/b using anti-ETF1a/b antibody (AZA8KB65). ETF1a/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ETF1a/b Antibody (AZA8KB65) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8kb65-etf1a-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish ETF1a/b Antibody Picoband® IHC analysis of ETF1a/b using anti-ETF1a/b antibody (AZA8KB65). ETF1a/b was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ETF1a/b Antibody (AZA8KB65) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fhl2a-b-antibody-azq6azb7-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azb7-fhl2a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish FHL2a/b Antibody IHC analysis of FHL2a/b using anti-FHL2a/b antibody (AZQ6AZB7). FHL2a/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FHL2a/b Antibody (AZQ6AZB7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azb7-fhl2a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish FHL2a/b Antibody IHC analysis of FHL2a/b using anti-FHL2a/b antibody (AZQ6AZB7). FHL2a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FHL2a/b Antibody (AZQ6AZB7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azb7-fhl2a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish FHL2a/b Antibody IHC analysis of FHL2a/b using anti-FHL2a/b antibody (AZQ6AZB7). FHL2a/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FHL2a/b Antibody (AZQ6AZB7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azb7-fhl2a-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish FHL2a/b Antibody IHC analysis of FHL2a/b using anti-FHL2a/b antibody (AZQ6AZB7). FHL2a/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FHL2a/b Antibody (AZQ6AZB7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azb7-fhl2a-b-primary-antibodies-if-testing-5.jpgAnti-Zebrafish FHL2a/b Antibody IF analysis of FHL2a/b using anti-FHL2a/b antibody (AZQ6AZB7). FHL2a/b was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FHL2a/b Antibody (AZQ6AZB7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azb7-fhl2a-b-primary-antibodies-if-testing-6.jpgAnti-Zebrafish FHL2a/b AntibodyIF analysis of FHL2a/b using anti-FHL2a/b antibody (AZQ6AZB7). FHL2a/b was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FHL2a/b Antibody (AZQ6AZB7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azb7-fhl2a-b-primary-antibodies-if-testing-7.jpgAnti-Zebrafish FHL2a/b AntibodyIF analysis of FHL2a/b using anti-FHL2a/b antibody (AZQ6AZB7). FHL2a/b was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FHL2a/b Antibody (AZQ6AZB7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gabarapa-b-antibody-azq6nzz7-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nzz7-gabarapa-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GABARAPa/b Antibody Picoband® Western blot analysis of GABARAPa/b using anti-GABARAPa/b antibody (AZQ6NZZ7). Electrophoresis was performed on a 12% SDS-PAGE SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABARAPa/b antigen affinity purified polyclonal antibody (AZQ6NZZ7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GABARAPa/b at approximately 18 kDa. The expected band size for GABARAPa/b is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nzz7-gabarapa-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GABARAPa/b Antibody Picoband® IHC analysis of GABARAPa/b using anti-GABARAPa/b antibody (AZQ6NZZ7). GABARAPa/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GABARAPa/b Antibody (AZQ6NZZ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nzz7-gabarapa-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish GABARAPa/b Antibody Picoband® IHC analysis of GABARAPa/b using anti-GABARAPa/b antibody (AZQ6NZZ7). GABARAPa/b was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GABARAPa/b Antibody (AZQ6NZZ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nzz7-gabarapa-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish GABARAPa/b Antibody Picoband® IHC analysis of GABARAPa/b using anti-GABARAPa/b antibody (AZQ6NZZ7). GABARAPa/b was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GABARAPa/b Antibody (AZQ6NZZ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nzz7-gabarapa-b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish GABARAPa/b Antibody Picoband® IHC analysis of GABARAPa/b using anti-GABARAPa/b antibody (AZQ6NZZ7). GABARAPa/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GABARAPa/b Antibody (AZQ6NZZ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gap43-antibody-azq90462-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90462-gap43-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GAP43 Antibody Picoband® Western blot analysis of GAP43 using anti-GAP43 antibody (AZQ90462). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAP43 antigen affinity purified polyclonal antibody (AZQ90462) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GAP43 at approximately 43 kDa. The expected band size for GAP43 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90462-gap43-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GAP43 Antibody Picoband® IHC analysis of GAP43 using anti-GAP43 antibody (AZQ90462). GAP43 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GAP43 Antibody (AZQ90462) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gbf1-antibody-aza0a286y9x1-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a286y9x1-gbf1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GBF1 Antibody Picoband® Western blot analysis of GBF1 using anti-GBF1 antibody (AZA0A286Y9X1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBF1 antigen affinity purified polyclonal antibody (AZA0A286Y9X1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GBF1 at approximately 207 kDa. The expected band size for GBF1 is at 207 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a286y9x1-gbf1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GBF1 Antibody Picoband® IHC analysis of GBF1 using anti-GBF1 antibody (AZA0A286Y9X1). GBF1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GBF1 Antibody (AZA0A286Y9X1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a286y9x1-gbf1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish GBF1 Antibody Picoband® IHC analysis of GBF1 using anti-GBF1 antibody (AZA0A286Y9X1). GBF1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GBF1 Antibody (AZA0A286Y9X1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a286y9x1-gbf1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish GBF1 Antibody Picoband® IHC analysis of GBF1 using anti-GBF1 antibody (AZA0A286Y9X1). GBF1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GBF1 Antibody (AZA0A286Y9X1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a286y9x1-gbf1-primary-antibodies-if-testing-5.jpgAnti-Zebrafish GBF1 Antibody Picoband® IF analysis of GBF1 using anti-GBF1 antibody (AZA0A286Y9X1). GBF1 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GBF1 Antibody (AZA0A286Y9X1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gipc1-antibody-azq6b337-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6b337-gipc1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GIPC1 Antibody Picoband® Western blot analysis of GIPC1 using anti-GIPC1 antibody (AZQ6B337). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIPC1 antigen affinity purified polyclonal antibody (AZQ6B337) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GIPC1 at approximately 40 kDa. The expected band size for GIPC1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6b337-gipc1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GIPC1 Antibody Picoband® IHC analysis of GIPC1 using anti-GIPC1 antibody (AZQ6B337). GIPC1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GIPC1 Antibody (AZQ6B337) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6b337-gipc1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish GIPC1 Antibody Picoband® IHC analysis of GIPC1 using anti-GIPC1 antibody (AZQ6B337). GIPC1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GIPC1 Antibody (AZQ6B337) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6b337-gipc1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish GIPC1 Antibody Picoband® IHC analysis of GIPC1 using anti-GIPC1 antibody (AZQ6B337). GIPC1 was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GIPC1 Antibody (AZQ6B337) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6b337-gipc1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish GIPC1 Antibody Picoband® IHC analysis of GIPC1 using anti-GIPC1 antibody (AZQ6B337). GIPC1 was detected in a paraffin-embedded section of zebrafish esophagus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GIPC1 Antibody (AZQ6B337) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-glsa-b-antibody-aza0a8m9prt8-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9prt8-glsa-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GLSa/b Antibody Picoband® Western blot analysis of GLSa/b using anti-GLSa/b antibody (AZA0A8M9PRT8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLSa/b antigen affinity purified polyclonal antibody (AZA0A8M9PRT8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GLSa/b at approximately 65 kDa. The expected band size for GLSa/b is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9prt8-glsa-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GLSa/b Antibody Picoband® IHC analysis of GLSa/b using anti-GLSa/b antibody (AZA0A8M9PRT8). GLSa/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLSa/b Antibody (AZA0A8M9PRT8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9prt8-glsa-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish GLSa/b Antibody Picoband® IHC analysis of GLSa/b using anti-GLSa/b antibody (AZA0A8M9PRT8). GLSa/b was detected in a paraffin-embedded section of zebrafish bile duct tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLSa/b Antibody (AZA0A8M9PRT8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9prt8-glsa-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish GLSa/b Antibody Picoband® IHC analysis of GLSa/b using anti-GLSa/b antibody (AZA0A8M9PRT8). GLSa/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLSa/b Antibody (AZA0A8M9PRT8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-glud1a-b-antibody-azq6nz29-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nz29-glud1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GLUD1a/b Antibody Picoband® Western blot analysis of GLUD1a/b using anti-GLUD1a/b antibody (AZQ6NZ29). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUD1a/b antigen affinity purified polyclonal antibody (AZQ6NZ29) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GLUD1a/b at approximately 55 kDa. The expected band size for GLUD1a/b is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nz29-glud1a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GLUD1a/b Antibody Picoband® IHC analysis of GLUD1a/b using anti-GLUD1a/b antibody (AZQ6NZ29). GLUD1a/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1a/b Antibody (AZQ6NZ29) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nz29-glud1a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish GLUD1a/b Antibody Picoband® IHC analysis of GLUD1a/b using anti-GLUD1a/b antibody (AZQ6NZ29). GLUD1a/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1a/b Antibody (AZQ6NZ29) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nz29-glud1a-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish GLUD1a/b Antibody Picoband® IHC analysis of GLUD1a/b using anti-GLUD1a/b antibody (AZQ6NZ29). GLUD1a/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUD1a/b Antibody (AZQ6NZ29) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-glula-b-antibody-azq7t2p7-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t2p7-glula-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GLULa/b Antibody Picoband® Western blot analysis of GLULa/b using anti-GLULa/b antibody (AZQ7T2P7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLULa/b antigen affinity purified polyclonal antibody (AZQ7T2P7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GLULa/b at approximately 42 kDa. The expected band size for GLULa/b is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t2p7-glula-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GLULa/b Antibody Picoband® IHC analysis of GLULa/b using anti-GLULa/b antibody (AZQ7T2P7). GLULa/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLULa/b Antibody (AZQ7T2P7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t2p7-glula-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish GLULa/b Antibody Picoband® IHC analysis of GLULa/b using anti-GLULa/b antibody (AZQ7T2P7). GLULa/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLULa/b Antibody (AZQ7T2P7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gmps-antibody-azb8jlw8-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jlw8-gmps-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish GMPS Antibody IHC analysis of GMPS using anti-GMPS antibody (AZB8JLW8). GMPS was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GMPS Antibody (AZB8JLW8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jlw8-gmps-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GMPS Antibody IHC analysis of GMPS using anti-GMPS antibody (AZB8JLW8). GMPS was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GMPS Antibody (AZB8JLW8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jlw8-gmps-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish GMPS Antibody IHC analysis of GMPS using anti-GMPS antibody (AZB8JLW8). GMPS was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GMPS Antibody (AZB8JLW8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gnao1a-antibody-azq6pbp1-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pbp1-gnao1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GNAO1a Antibody Picoband® Western blot analysis of GNAO1a using anti-GNAO1a antibody (AZQ6PBP1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAO1a antigen affinity purified polyclonal antibody (AZQ6PBP1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GNAO1a at approximately 40 kDa. The expected band size for GNAO1a is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pbp1-gnao1a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GNAO1a Antibody Picoband® IHC analysis of GNAO1a using anti-GNAO1a antibody (AZQ6PBP1). GNAO1a was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNAO1a Antibody (AZQ6PBP1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pbp1-gnao1a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish GNAO1a Antibody Picoband® IHC analysis of GNAO1a using anti-GNAO1a antibody (AZQ6PBP1). GNAO1a was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNAO1a Antibody (AZQ6PBP1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pbp1-gnao1a-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish GNAO1a Antibody Picoband® IHC analysis of GNAO1a using anti-GNAO1a antibody (AZQ6PBP1). GNAO1a was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNAO1a Antibody (AZQ6PBP1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pbp1-gnao1a-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish GNAO1a Antibody Picoband® IHC analysis of GNAO1a using anti-GNAO1a antibody (AZQ6PBP1). GNAO1a was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNAO1a Antibody (AZQ6PBP1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pbp1-gnao1a-primary-antibodies-if-testing-6.jpgAnti-Zebrafish GNAO1a Antibody Picoband® IF analysis of GNAO1a using anti-GNAO1a antibody (AZQ6PBP1). GNAO1a was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GNAO1a Antibody (AZQ6PBP1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gnb1-1b-antibody-azq6ph57-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph57-gnb1-1b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GNB1/1b Antibody Picoband® Western blot analysis of GNB1/1b using anti-GNB1/1b antibody (AZQ6PH57). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNB1/1b antigen affinity purified polyclonal antibody (AZQ6PH57) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GNB1/1b at approximately 37 kDa. The expected band size for GNB1/1b is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph57-gnb1-1b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GNB1/1b Antibody Picoband® IHC analysis of GNB1/1b using anti-GNB1/1b antibody (AZQ6PH57). GNB1/1b was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNB1/1b Antibody (AZQ6PH57) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph57-gnb1-1b-primary-antibodies-if-testing-3.jpgAnti-Zebrafish GNB1/1b Antibody Picoband® IF analysis of GNB1/1b using anti-GNB1/1b antibody (AZQ6PH57). GNB1/1b was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GNB1/1b Antibody (AZQ6PH57) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ph57-gnb1-1b-primary-antibodies-if-testing-4.jpgAnti-Zebrafish GNB1/1b Antibody Picoband®IF analysis of GNB1/1b using anti-GNB1/1b antibody (AZQ6PH57). GNB1/1b was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GNB1/1b Antibody (AZQ6PH57) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gnsa-antibody-azq4v902-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq4v902-gnsa-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GNSa Antibody Picoband® Western blot analysis of GNSa using anti-GNSa antibody (AZQ4V902). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNSa antigen affinity purified polyclonal antibody (AZQ4V902) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GNSa at approximately 95 kDa. The expected band size for GNSa is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq4v902-gnsa-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GNSa Antibody Picoband® IHC analysis of GNSa using anti-GNSa antibody (AZQ4V902). GNSa was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNSa Antibody (AZQ4V902) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq4v902-gnsa-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish GNSa Antibody Picoband® IHC analysis of GNSa using anti-GNSa antibody (AZQ4V902). GNSa was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNSa Antibody (AZQ4V902) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq4v902-gnsa-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish GNSa Antibody Picoband® IHC analysis of GNSa using anti-GNSa antibody (AZQ4V902). GNSa was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNSa Antibody (AZQ4V902) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gphna-b-antibody-azd3kyk7-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azd3kyk7-gphna-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GPHNa/b Antibody Picoband® Western blot analysis of GPHNa/b using anti-GPHNa/b antibody (AZD3KYK7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPHNa/b antigen affinity purified polyclonal antibody (AZD3KYK7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GPHNa/b at approximately 97 kDa. The expected band size for GPHNa/b is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azd3kyk7-gphna-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GPHNa/b Antibody Picoband® IHC analysis of GPHNa/b using anti-GPHNa/b antibody (AZD3KYK7). GPHNa/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPHNa/b Antibody (AZD3KYK7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azd3kyk7-gphna-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish GPHNa/b Antibody Picoband® IHC analysis of GPHNa/b using anti-GPHNa/b antibody (AZD3KYK7). GPHNa/b was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPHNa/b Antibody (AZD3KYK7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azd3kyk7-gphna-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish GPHNa/b Antibody Picoband® IHC analysis of GPHNa/b using anti-GPHNa/b antibody (AZD3KYK7). GPHNa/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPHNa/b Antibody (AZD3KYK7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azd3kyk7-gphna-b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish GPHNa/b Antibody Picoband® IHC analysis of GPHNa/b using anti-GPHNa/b antibody (AZD3KYK7). GPHNa/b was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPHNa/b Antibody (AZD3KYK7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-grin1a-b-antibody-azf1r366-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r366-grin1a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish GRIN1a/b Antibody IHC analysis of GRIN1a/b using anti-GRIN1a/b antibody (AZF1R366). GRIN1a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRIN1a/b Antibody (AZF1R366) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-grpel1-antibody-azq32ps5-boster.html2026-03-17T05:16:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq32ps5-grpel1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GRPEL1 Antibody Picoband® Western blot analysis of GRPEL1 using anti-GRPEL1 antibody (AZQ32PS5). Electrophoresis was performed on a 12% SDS-PAGE SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRPEL1 antigen affinity purified polyclonal antibody (AZQ32PS5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRPEL1 at approximately 24 kDa. The expected band size for GRPEL1 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq32ps5-grpel1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GRPEL1 Antibody Picoband® IHC analysis of GRPEL1 using anti-GRPEL1 antibody (AZQ32PS5). GRPEL1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRPEL1 Antibody (AZQ32PS5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-histone-h2a-x-h2afx-antibody-azq7zuy3-boster.html2026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zuy3-histone-h2ax-h2afx-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Histone H2A.X/H2AFX Antibody Picoband® Western blot analysis of Histone H2A.X/H2AFX using anti-Histone H2A.X/H2AFX antibody (AZQ7ZUY3). Electrophoresis was performed on a 12% SDS-PAGE SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H2A.X/H2AFX antigen affinity purified polyclonal antibody (AZQ7ZUY3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Histone H2A.X/H2AFX at approximately 15 kDa. The expected band size for Histone H2A.X/H2AFX is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zuy3-histone-h2ax-h2afx-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Histone H2A.X/H2AFX Antibody Picoband® IHC analysis of Histone H2A.X/H2AFX using anti-Histone H2A.X/H2AFX antibody (AZQ7ZUY3). Histone H2A.X/H2AFX was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H2A.X/H2AFX Antibody (AZQ7ZUY3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zuy3-histone-h2ax-h2afx-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish Histone H2A.X/H2AFX Antibody Picoband® IHC analysis of Histone H2A.X/H2AFX using anti-Histone H2A.X/H2AFX antibody (AZQ7ZUY3). Histone H2A.X/H2AFX was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H2A.X/H2AFX Antibody (AZQ7ZUY3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zuy3-histone-h2ax-h2afx-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish Histone H2A.X/H2AFX Antibody Picoband® IHC analysis of Histone H2A.X/H2AFX using anti-Histone H2A.X/H2AFX antibody (AZQ7ZUY3). Histone H2A.X/H2AFX was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H2A.X/H2AFX Antibody (AZQ7ZUY3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hdac1-antibody-aza0a2r8qiw0-boster.html2026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8qiw0-hdac1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HDAC1 Antibody IHC analysis of HDAC1 using anti-HDAC1 antibody (AZA0A2R8QIW0). HDAC1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HDAC1 Antibody (AZA0A2R8QIW0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8qiw0-hdac1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HDAC1 Antibody IHC analysis of HDAC1 using anti-HDAC1 antibody (AZA0A2R8QIW0). HDAC1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HDAC1 Antibody (AZA0A2R8QIW0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-histone-h3-antibody-azq6pi20-boster.html2026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pi20-histone-h3-primary-antibodies-if-testing-1.jpgAnti-Zebrafish Histone H3 Antibody Picoband® IF analysis of Histone H3 using anti-Histone H3 antibody (AZQ6PI20). Histone H3 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Histone H3 Antibody (AZQ6PI20) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pi20-histone-h3-primary-antibodies-wb-testing-2.jpgAnti-Zebrafish Histone H3 Antibody Picoband® Western blot analysis of Histone H3 using anti-Histone H3 antibody (AZQ6PI20). Electrophoresis was performed on a 12% SDS-PAGE SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 antigen affinity purified polyclonal antibody (AZQ6PI20) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Histone H3 at approximately 15 kDa. The expected band size for Histone H3 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pi20-histone-h3-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish Histone H3 Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (AZQ6PI20). Histone H3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (AZQ6PI20) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pi20-histone-h3-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish Histone H3 Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (AZQ6PI20). Histone H3 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (AZQ6PI20) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pi20-histone-h3-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish Histone H3 Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (AZQ6PI20). Histone H3 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (AZQ6PI20) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pi20-histone-h3-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish Histone H3 Antibody Picoband® IHC analysis of Histone H3 using anti-Histone H3 antibody (AZQ6PI20). Histone H3 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Histone H3 Antibody (AZQ6PI20) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hmgb1a-b-antibody-azq6nx86-boster.html2026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nx86-hmgb1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HMGB1a/b AntibodyWestern blot analysis of HMGB1a/b using anti-HMGB1a/b antibody (AZQ6NX86). Electrophoresis was performed on a 10% SDS-PAGE SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGB1a/b antigen affinity purified polyclonal antibody (AZQ6NX86) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HMGB1a/b at approximately 23 kDa. The expected band size for HMGB1a/b is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nx86-hmgb1a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HMGB1a/b Antibody IHC analysis of HMGB1a/b using anti-HMGB1a/b antibody (AZQ6NX86). HMGB1a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGB1a/b Antibody (AZQ6NX86) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nx86-hmgb1a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HMGB1a/b AntibodyIHC analysis of HMGB1a/b using anti-HMGB1a/b antibody (AZQ6NX86). HMGB1a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGB1a/b Antibody (AZQ6NX86) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nx86-hmgb1a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HMGB1a/b AntibodyIHC analysis of HMGB1a/b using anti-HMGB1a/b antibody (AZQ6NX86). HMGB1a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGB1a/b Antibody (AZQ6NX86) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nx86-hmgb1a-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish HMGB1a/b AntibodyIHC analysis of HMGB1a/b using anti-HMGB1a/b antibody (AZQ6NX86). HMGB1a/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGB1a/b Antibody (AZQ6NX86) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nx86-hmgb1a-b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish HMGB1a/b AntibodyIHC analysis of HMGB1a/b using anti-HMGB1a/b antibody (AZQ6NX86). HMGB1a/b was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGB1a/b Antibody (AZQ6NX86) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hmgb2a-b-antibody-azq32pt3-boster.html2026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq32pt3-hmgb2a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HMGB2a/b Antibody Picoband® Western blot analysis of HMGB2a/b using anti-HMGB2a/b antibody (AZQ32PT3). Electrophoresis was performed on a 12% SDS-PAGE SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGB2a/b antigen affinity purified polyclonal antibody (AZQ32PT3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HMGB2a/b at approximately 24 kDa. The expected band size for HMGB2a/b is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq32pt3-hmgb2a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HMGB2a/b Antibody Picoband® IHC analysis of HMGB2a/b using anti-HMGB2a/b antibody (AZQ32PT3). HMGB2a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGB2a/b Antibody (AZQ32PT3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq32pt3-hmgb2a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HMGB2a/b Antibody Picoband® IHC analysis of HMGB2a/b using anti-HMGB2a/b antibody (AZQ32PT3). HMGB2a/b was detected in a paraffin-embedded section of zebrafish esophagus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGB2a/b Antibody (AZQ32PT3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq32pt3-hmgb2a-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish HMGB2a/b Antibody Picoband® IHC analysis of HMGB2a/b using anti-HMGB2a/b antibody (AZQ32PT3). HMGB2a/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGB2a/b Antibody (AZQ32PT3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq32pt3-hmgb2a-b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish HMGB2a/b Antibody Picoband® IHC analysis of HMGB2a/b using anti-HMGB2a/b antibody (AZQ32PT3). HMGB2a/b was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGB2a/b Antibody (AZQ32PT3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1295502026-03-13T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01524-2-lc3b-map1lc3b-primary-antibodies-wb-testing-1.jpgAnti-LC3B/MAP1LC3B Antibody Western blot analysis of LC3B/MAP1LC3B using anti-LC3B/MAP1LC3B antibody (A01524-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LC3B/MAP1LC3B antigen affinity purified polyclonal antibody (A01524-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LC3B/MAP1LC3B at approximately 15,18 kDa. The expected band size for LC3B/MAP1LC3B is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01524-2-lc3b-map1lc3b-primary-antibodies-ihc-testing-2.jpgAnti-LC3B/MAP1LC3B Antibody IHC analysis of LC3B/MAP1LC3B using anti-LC3B/MAP1LC3B antibody (A01524-2). LC3B/MAP1LC3B was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-LC3B/MAP1LC3B Antibody (A01524-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01524-2-lc3b-map1lc3b-primary-antibodies-ihc-testing-3.jpgAnti-LC3B/MAP1LC3B Antibody IHC analysis of LC3B/MAP1LC3B using anti-LC3B/MAP1LC3B antibody (A01524-2). LC3B/MAP1LC3B was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-LC3B/MAP1LC3B Antibody (A01524-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01524-2-lc3b-map1lc3b-primary-antibodies-ihc-testing-4.jpgAnti-LC3B/MAP1LC3B Antibody IHC analysis of LC3B/MAP1LC3B using anti-LC3B/MAP1LC3B antibody (A01524-2). LC3B/MAP1LC3B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-LC3B/MAP1LC3B Antibody (A01524-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01524-2-13195_2025_1714_fig7_html.pngAnti-LC3B/MAP1LC3B AntibodyPBM alleviates abnormal mitochondrial autophagy and promotes mitochondrial energy metabolism in mice with AD. A RT-PCR was used to detect autophagy-related protein (Beclin1, LC3II.) and glycolysis-related proteins (TSPO and HK2) in mouse brain tissues. B WB was used to detect the autophagy protein LC3II, and the oxidative phosphorylation-related proteins PGC-1α and NRF-1. C Immunohistochemical staining of mouse brains using an anti-HK2 antibody (scale bars 100 μm and 20 μm, respectively) with brown plaques of HK2. D , E WB was used to detect the glycolysis-related proteins GLUT1, PKM2, and HK2. Experimental data are presented as the mean ± standard deviation. Compared with the CON group, * p < 0.05, ** p < 0.01, *** p < 0.001; Compared with the AD group, # p < 0.05, ## p < 0.01 Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13195-025-01714-w'>40188044</a> https://www.bosterbio.com/catalog/product/view/id/1295512026-03-13T05:05:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295522026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04890-2-rab27b-primary-antibodies-wb-testing-1.jpgAnti-RAB27B Antibody Picoband® Western blot analysis of RAB27B using anti-RAB27B antibody (A04890-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB27B antigen affinity purified polyclonal antibody (A04890-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB27B at approximately 25 kDa. The expected band size for RAB27B is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04890-2-rab27b-primary-antibodies-ihc-testing-2.jpgAnti-RAB27B Antibody Picoband® IHC analysis of RAB27B using anti-RAB27B antibody (A04890-2). RAB27B was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB27B Antibody (A04890-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04890-2-rab27b-primary-antibodies-ihc-testing-3.jpgAnti-RAB27B Antibody Picoband® IHC analysis of RAB27B using anti-RAB27B antibody (A04890-2). RAB27B was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB27B Antibody (A04890-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04890-2-rab27b-primary-antibodies-ihc-testing-4.jpgAnti-RAB27B Antibody Picoband® IHC analysis of RAB27B using anti-RAB27B antibody (A04890-2). RAB27B was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB27B Antibody (A04890-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04890-2-rab27b-primary-antibodies-if-testing-5.jpgAnti-RAB27B Antibody Picoband® IF analysis of RAB27B using anti-RAB27B antibody (A04890-2). RAB27B was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-RAB27B Antibody (A04890-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04890-2-rab27b-primary-antibodies-if-testing-6.jpgAnti-RAB27B Antibody Picoband® IF analysis of RAB27B using anti-RAB27B antibody (A04890-2). RAB27B was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/ml rabbit anti-RAB27B Antibody (A04890-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1295532026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295542026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295552026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295562026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295572026-03-13T05:05:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295582026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295592026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295602026-03-13T05:05:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295612026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295622026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295632026-03-13T05:05:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295642026-03-13T05:05:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295652026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03564-5-creb3-primary-antibodies-wb-testing-1.jpgAnti-CREB3 Antibody Picoband®Western blot analysis of CREB3 using anti-CREB3 antibody (A03564-5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U251 whole cell lysates Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB3 antigen affinity purified polyclonal antibody (A03564-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CREB3 at approximately 41 kDa. The expected band size for CREB3 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03564-5-creb3-primary-antibodies-ip-testing-1.jpgAnti-CREB3 Antibody Picoband®Immunoprecipitating CREB3 in A549 whole cell lysate. Western blot analysis of CREB3 using anti-CREB3 antibody (A03564-5). Lane 1: A549 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-CREB3 antibody in A549 whole cell lysate, Lane 3: anti-CREB3 antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CREB3 antigen affinity purified polyclonal antibody (A03564-5) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CREB3 at approximately 45 kDa. The expected band size for CREB3 is at 41 kDa. https://www.bosterbio.com/catalog/product/view/id/1295662026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295672026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295682026-03-13T05:05:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295692026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295702026-03-13T05:05:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295712026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295722026-03-16T05:08:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295732026-03-13T05:05:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295742026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295752026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295762026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295772026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295782026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295792026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295802026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295812026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295822026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06821-2-nefm-primary-antibodies-wb-testing-1.jpgAnti-NF-M/NEFM Antibody Picoband®Western blot analysis of NF-M/NEFM using anti-NF-M/NEFM antibody (A06821-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-M/NEFM antigen affinity purified polyclonal antibody (A06821-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NF-M/NEFM at approximately 160 kDa. The expected band size for NF-M/NEFM is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06821-2-nefm-primary-antibodies-ihc-testing-5.jpgAnti-NF-M/NEFM Antibody Picoband®IHC analysis of NF-M/NEFM using anti-NF-M/NEFM antibody (A06821-2). NF-M/NEFM was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF-M/NEFM Antibody (A06821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06821-2-nefm-primary-antibodies-ihc-testing-6.jpgAnti-NF-M/NEFM Antibody Picoband®IHC analysis of NF-M/NEFM using anti-NF-M/NEFM antibody (A06821-2). NF-M/NEFM was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF-M/NEFM Antibody (A06821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06821-2-nefm-primary-antibodies-ihc-testing-4.jpgAnti-NF-M/NEFM Antibody Picoband®IHC analysis of NF-M/NEFM using anti-NF-M/NEFM antibody (A06821-2). NF-M/NEFM was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF-M/NEFM Antibody (A06821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06821-2-nefm-primary-antibodies-ihc-testing-3.jpgAnti-NF-M/NEFM Antibody Picoband®IHC analysis of NF-M/NEFM using anti-NF-M/NEFM antibody (A06821-2). NF-M/NEFM was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF-M/NEFM Antibody (A06821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06821-2-nefm-primary-antibodies-ihc-testing-2.jpgAnti-NF-M/NEFM Antibody Picoband®IHC analysis of NF-M/NEFM using anti-NF-M/NEFM antibody (A06821-2). NF-M/NEFM was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF-M/NEFM Antibody (A06821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06821-2-nefm-primary-antibodies-ihc-testing-1.jpgAnti-NF-M/NEFM Antibody Picoband®IHC analysis of NF-M/NEFM using anti-NF-M/NEFM antibody (A06821-2). NF-M/NEFM was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF-M/NEFM Antibody (A06821-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06821-2-nefm-primary-antibodies-ip-testing-1.jpgAnti-NF-M/NEFM Antibody Picoband®Immunoprecipitating NF-M/NEFM in SH-SY5Y whole cell lysate. Western blot analysis of NF-M/NEFM using anti-NF-M/NEFM antibody (A06821-2). Lane 1: SH-SY5Y whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-NF-M/NEFM antibody in SH-SY5Y whole cell lysate, Lane 3: anti-NF-M/NEFM antibody (2μg) + SH-SY5Y whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NF-M/NEFM antigen affinity purified polyclonal antibody (A06821-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NF-M/NEFM at approximately 160 kDa. The expected band size for NF-M/NEFM is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06821-2-nefm-primary-antibodies-if-testing-1.jpgAnti-NF-M/NEFM Antibody Picoband®IF analysis of NF-M/NEFM using anti-NF-M/NEFM antibody (A06821-2). NF-M/NEFM was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NF-M/NEFM Antibody (A06821-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06821-2-nefm-primary-antibodies-if-testing-2.jpgAnti-NF-M/NEFM Antibody Picoband®IF analysis of NF-M/NEFM using anti-NF-M/NEFM antibody (A06821-2). NF-M/NEFM was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NF-M/NEFM Antibody (A06821-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06821-2-nefm-primary-antibodies-fcm-testing-1.jpgAnti-NF-M/NEFM Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-NF-M/NEFM antibody (A06821-2). Overlay histogram showing 293T cells stained with A06821-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NF-M/NEFM Antibody (A06821-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1295832026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02714-1-pde4a-primary-antibodies-ihc-testing-1.jpgAnti-PDE4A AntibodyIHC analysis of PDE4A using anti-PDE4A antibody (A02714-1). PDE4A was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDE4A Antibody (A02714-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02714-1-pde4a-primary-antibodies-ihc-testing-2.jpgAnti-PDE4A AntibodyIHC analysis of PDE4A using anti-PDE4A antibody (A02714-1). PDE4A was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDE4A Antibody (A02714-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02714-1-pde4a-primary-antibodies-if-testing-1.jpgAnti-PDE4A AntibodyIF analysis of PDE4A using anti-PDE4A antibody (A02714-1). PDE4A was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PDE4A Antibody (A02714-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1295852026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295862026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295872026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295882026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295892026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295902026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295912026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295922026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295932026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02749-1-sco2-primary-antibodies-wb-testing-1.jpgAnti-SCO2 Antibody Picoband®Western blot analysis of SCO2 using anti-SCO2 antibody (A02749-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCO2 antigen affinity purified polyclonal antibody (A02749-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SCO2 at approximately 27 kDa. The expected band size for SCO2 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02749-1-sco2-primary-antibodies-fcm-testing-1.jpgAnti-SCO2 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-SCO2 antibody (A02749-1). Overlay histogram showing PC-3 cells stained with A02749-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCO2 Antibody (A02749-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1295942026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295952026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02942-2-2-sra1-primary-antibodies-ihc-testing-1.jpgAnti-SRA1 Antibody Picoband®IHC analysis of SRA1 using anti-SRA1 antibody (A02942-2). SRA1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-SRA1 Antibody (A02942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02942-2-sra1-primary-antibodies-wb-testing-1.jpgAnti-SRA1 Antibody Picoband®Western blot analysis of SRA1 using anti-SRA1 antibody (A02942-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A2780 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRA1 antigen affinity purified polyclonal antibody (A02942-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SRA1 at approximately 33 kDa. The expected band size for SRA1 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02942-2-sra1-primary-antibodies-if-testing-1.jpgAnti-SRA1 Antibody Picoband®IF analysis of SRA1 using anti-SRA1 antibody (A02942-2) and anti-Beta Tubulin antibody (M01857-3). SRA1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SRA1 Antibody (A02942-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02942-2-sra1-primary-antibodies-fcm-testing-1.jpgAnti-SRA1 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-SRA1 antibody (A02942-2). Overlay histogram showing 293T cells stained with A02942-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRA1 Antibody (A02942-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02942-2-2-sra1-primary-antibodies-ihc-testing-2.jpgAnti-SRA1 Antibody Picoband®IHC analysis of SRA1 using anti-SRA1 antibody (A02942-2). SRA1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-SRA1 Antibody (A02942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02942-2-2-sra1-primary-antibodies-ihc-testing-3.jpgAnti-SRA1 Antibody Picoband®IHC analysis of SRA1 using anti-SRA1 antibody (A02942-2). SRA1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-SRA1 Antibody (A02942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02942-2-2-sra1-primary-antibodies-ihc-testing-4.jpgAnti-SRA1 Antibody Picoband®IHC analysis of SRA1 using anti-SRA1 antibody (A02942-2). SRA1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-SRA1 Antibody (A02942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02942-2-2-sra1-primary-antibodies-ihc-testing-5.jpgAnti-SRA1 Antibody Picoband®IHC analysis of SRA1 using anti-SRA1 antibody (A02942-2). SRA1 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-SRA1 Antibody (A02942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02942-2-2-sra1-primary-antibodies-ihc-testing-6.jpgAnti-SRA1 Antibody Picoband®IHC analysis of SRA1 using anti-SRA1 antibody (A02942-2). SRA1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-SRA1 Antibody (A02942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02942-2-2-sra1-primary-antibodies-ihc-testing-7.jpgAnti-SRA1 Antibody Picoband®IHC analysis of SRA1 using anti-SRA1 antibody (A02942-2). SRA1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-SRA1 Antibody (A02942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02942-2-2-sra1-primary-antibodies-ihc-testing-8.jpgAnti-SRA1 Antibody Picoband®IHC analysis of SRA1 using anti-SRA1 antibody (A02942-2). SRA1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-SRA1 Antibody (A02942-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1295962026-03-16T05:08:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295972026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1295982026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07212-2-sult1c2-primary-antibodies-wb-testing-1.jpgAnti-SULT1C2 Antibody Picoband®Western blot analysis of SULT1C2 using anti-SULT1C2 antibody (A07212-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse stomach tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT1C2 antigen affinity purified polyclonal antibody (A07212-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SULT1C2 at approximately 35 kDa. The expected band size for SULT1C2 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07212-2-sult1c2-primary-antibodies-fcm-testing-1.jpgAnti-SULT1C2 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-SULT1C2 antibody (A07212-2). Overlay histogram showing RT4 cells stained with A07212-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SULT1C2 Antibody (A07212-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1295992026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296002026-03-16T05:08:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09263-tbc1d5-primary-antibodies-ihc-testing-1.jpgAnti-TBC1D5 AntibodyIHC analysis of TBC1D5 using anti-TBC1D5 antibody (A09263). TBC1D5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-TBC1D5 Antibody (A09263) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09263-tbc1d5-primary-antibodies-ihc-testing-2.jpgAnti-TBC1D5 AntibodyIHC analysis of TBC1D5 using anti-TBC1D5 antibody (A09263). TBC1D5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-TBC1D5 Antibody (A09263) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09263-tbc1d5-primary-antibodies-ihc-testing-3.jpgAnti-TBC1D5 AntibodyIHC analysis of TBC1D5 using anti-TBC1D5 antibody (A09263). TBC1D5 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-TBC1D5 Antibody (A09263) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09263-tbc1d5-primary-antibodies-ihc-testing-4.jpgAnti-TBC1D5 AntibodyIHC analysis of TBC1D5 using anti-TBC1D5 antibody (A09263). TBC1D5 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-TBC1D5 Antibody (A09263) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09263-tbc1d5-primary-antibodies-ihc-testing-5.jpgAnti-TBC1D5 AntibodyIHC analysis of TBC1D5 using anti-TBC1D5 antibody (A09263). TBC1D5 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-TBC1D5 Antibody (A09263) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1296012026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296022026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296032026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296042026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296052026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296062026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09683-1-ubxn1-primary-antibodies-wb-testing-1.jpgAnti-UBXN1 Antibody Picoband® Western blot analysis of UBXN1 using anti-UBXN1 antibody (A09683-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: rat RH-35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBXN1 antigen affinity purified polyclonal antibody (A09683-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBXN1 at approximately 40 kDa. The expected band size for UBXN1 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09683-1-ubxn1-primary-antibodies-ihc-testing-1.jpgAnti-UBXN1 Antibody Picoband®IHC analysis of UBXN1 using anti-UBXN1 antibody (A09683-1). UBXN1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBXN1 Antibody (A09683-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09683-1-ubxn1-primary-antibodies-wb-testing-1_1.jpgAnti-UBXN1 Antibody Picoband®Western blot analysis of UBXN1 using anti-UBXN1 antibody (A09683-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Ramos whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBXN1 antigen affinity purified polyclonal antibody (A09683-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBXN1 at approximately 40 kDa. The expected band size for UBXN1 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09683-1-ubxn1-primary-antibodies-ihc-testing-1_1.jpgAnti-UBXN1 Antibody Picoband®IHC analysis of UBXN1 using anti-UBXN1 antibody (A09683-1). UBXN1 was detected in a paraffin-embedded section of human prostate hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBXN1 Antibody (A09683-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09683-1-ubxn1-primary-antibodies-ihc-testing-2.jpgAnti-UBXN1 Antibody Picoband®IHC analysis of UBXN1 using anti-UBXN1 antibody (A09683-1). UBXN1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBXN1 Antibody (A09683-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09683-1-ubxn1-primary-antibodies-ihc-testing-3.jpgAnti-UBXN1 Antibody Picoband®IHC analysis of UBXN1 using anti-UBXN1 antibody (A09683-1). UBXN1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBXN1 Antibody (A09683-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09683-1-ubxn1-primary-antibodies-ihc-testing-4.jpgAnti-UBXN1 Antibody Picoband®IHC analysis of UBXN1 using anti-UBXN1 antibody (A09683-1). UBXN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBXN1 Antibody (A09683-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09683-1-ubxn1-primary-antibodies-ip-testing-1.jpgAnti-UBXN1 Antibody Picoband®Immunoprecipitating UBXN1 in Hela whole cell lysate. Western blot analysis of UBXN1 using anti-UBXN1 antibody (A09683-1). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-UBXN1 antibody in Hela whole cell lysate, Lane 3: anti-UBXN1 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UBXN1 antigen affinity purified polyclonal antibody (A09683-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for UBXN1 at approximately 40 kDa. The expected band size for UBXN1 is at 33 kDa. https://www.bosterbio.com/catalog/product/view/id/1296072026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296082026-03-16T05:08:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06275-3-usp20-primary-antibodies-wb-testing-1_1.jpgAnti-USP20 AntibodyWestern blot analysis of USP20 using anti-USP20 antibody (A06275-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP20 antigen affinity purified polyclonal antibody (A06275-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for USP20 at approximately 120 kDa. The expected band size for USP20 is at 102 kDa. https://www.bosterbio.com/catalog/product/view/id/1296092026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296102026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296112026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296122026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296132026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296142026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296152026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296162026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296172026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296182026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296192026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11008-3-atp6v1h-primary-antibodies-wb-testing-1.jpgAnti-ATP6V1H Antibody Picoband®Western blot analysis of ATP6V1H using anti-ATP6V1H antibody (A11008-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1H antigen affinity purified polyclonal antibody (A11008-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATP6V1H at approximately 50 kDa. The expected band size for ATP6V1H is at 56 kDa. https://www.bosterbio.com/catalog/product/view/id/1296202026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296212026-03-16T05:08:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296222026-03-16T05:08:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296232026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296242026-03-16T05:08:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06956-1-cotl1-primary-antibodies-wb-testing-1.jpgAnti-COTL1 AntibodyWestern blot analysis of -COTL1 using anti-COTL1 antibody (A06956-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Siha whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COTL1 antigen affinity purified polyclonal antibody (A06956-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for COTL1 at approximately 16 kDa. The expected band size for COTL1 is at 16 kDa. https://www.bosterbio.com/catalog/product/view/id/1296252026-03-16T05:08:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05254-cplx1-primary-antibodies-wb-testing-1.jpgAnti-CPLX1 AntibodyWestern blot analysis of CPLX1 using anti-CPLX1 antibody (A05254). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPLX1 antigen affinity purified polyclonal antibody (A05254) at 1: 1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CPLX1 at approximately 20 kDa. The expected band size for CPLX1 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05254-cplx1-primary-antibodies-ihc-testing-1.jpgAnti-CPLX1 AntibodyIHC analysis of CPLX1 using anti-CPLX1 antibody (A05254). CPLX1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 200 rabbit anti-CPLX1 Antibody (A05254) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1296262026-03-16T05:08:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296272026-03-16T05:08:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296282026-03-16T05:08:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296292026-03-16T05:08:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296302026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07835-1-dbr1-primary-antibodies-wb-testing-1.jpgAnti-DBR1 Antibody Picoband®Western blot analysis of DBR1 using anti-DBR1 antibody (A07835-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBR1 antigen affinity purified polyclonal antibody (A07835-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DBR1 at approximately 75 kDa. The expected band size for DBR1 is at 62 kDa. https://www.bosterbio.com/catalog/product/view/id/1296312026-04-02T05:01:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12563-dctn5-primary-antibodies-wb-testing-1.jpgAnti-DCTN5 Antibody Picoband®Western blot analysis of DCTN5 using anti-DCTN5 antibody (A12563). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCTN5 antigen affinity purified polyclonal antibody (A12563) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCTN5 at approximately 16-20 kDa. The expected band size for DCTN5 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12563-dctn5-primary-antibodies-ihc-testing-1.jpgAnti-DCTN5 Antibody Picoband®IHC analysis of DCTN5 using anti-DCTN5 antibody (A12563). DCTN5 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DCTN5 Antibody (A12563) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12563-dctn5-primary-antibodies-ihc-testing-2.jpgAnti-DCTN5 Antibody Picoband®IHC analysis of DCTN5 using anti-DCTN5 antibody (A12563). DCTN5 was detected in a paraffin-embedded section of pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DCTN5 Antibody (A12563) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1296322026-03-16T05:08:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296332026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05291-3-ddx20-primary-antibodies-wb-testing-1.jpgAnti-DDX20 Antibody Picoband®Western blot analysis of DDX20 using anti-DDX20 antibody (A05291-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX20 antigen affinity purified polyclonal antibody (A05291-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX20 at approximately 110 kDa. The expected band size for DDX20 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05291-3-ddx20-primary-antibodies-if-testing-1.jpgAnti-DDX20 Antibody Picoband®IF analysis of DDX20 using anti-DDX20 antibody (A05291-3) and anti-Alpha Tubulin antibody (M03989-3). DDX20 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDX20 Antibody (A05291-3) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05291-3-ddx20-primary-antibodies-fcm-testing-1.jpgAnti-DDX20 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-DDX20 antibody (A05291-3). Overlay histogram showing SH-SY5Y cells stained with A05291-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX20 Antibody (A05291-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1296342026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10426-1-dhx38-primary-antibodies-wb-testing-1.jpgAnti-DHX38 Antibody Picoband®Western blot analysis of DHX38 using anti-DHX38 antibody (A10426-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX38 antigen affinity purified polyclonal antibody (A10426-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHX38 at approximately 150 kDa. The expected band size for DHX38 is at 141 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10426-1-dhx38-primary-antibodies-if-testing-1.jpgAnti-DHX38 Antibody Picoband®IF analysis of DHX38 using anti-DHX38 antibody (A10426-1) and anti-Alpha Tubulin antibody (M03989-3). DHX38 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DHX38 Antibody (A10426-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10426-1-dhx38-primary-antibodies-fcm-testing-1.jpgAnti-DHX38 Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-DHX38 antibody (A10426-1). Overlay histogram showing Caco-2 cells stained with A10426-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DHX38 Antibody (A10426-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1296352026-03-16T05:08:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296362026-04-02T05:01:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11284-1-dusp11-primary-antibodies-wb-testing-1.jpgAnti-DUSP11 Antibody Picoband®Western blot analysis of DUSP11 using anti-DUSP11 antibody (A11284-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP11 antigen affinity purified polyclonal antibody (A11284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DUSP11 at approximately 39 kDa. The expected band size for DUSP11 is at 39 kDa. https://www.bosterbio.com/catalog/product/view/id/1296372026-03-16T05:08:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296382026-04-02T05:01:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05450-eif3i-primary-antibodies-wb-testing-1.jpgAnti-EIF3I Antibody Picoband®Western blot analysis of EIF3I using anti-EIF3I antibody (A05450). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3I antigen affinity purified polyclonal antibody (A05450) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EIF3I at approximately 37 kDa. The expected band size for EIF3I is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05450-eif3i-primary-antibodies-ihc-testing-1.jpgAnti-EIF3I Antibody Picoband®IHC analysis of EIF3I using anti-EIF3I antibody (A05450). EIF3I was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3I Antibody (A05450) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1296392026-03-16T05:08:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296402026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296412026-03-16T05:08:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06068-1-esrp1-primary-antibodies-wb-testing-1.jpgAnti-ESRP1 AntibodyWestern blot analysis of ESRP1 using anti-ESRP1 antibody (A06068-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESRP1 antigen affinity purified polyclonal antibody (A06068-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ESRP1 at approximately 76 kDa. The expected band size for ESRP1 is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06068-1-esrp1-primary-antibodies-wb-testing-1_1.jpgAnti-ESRP1 AntibodyWestern blot analysis of ESRP1 using anti-ESRP1 antibody (A06068-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESRP1 antigen affinity purified polyclonal antibody (A06068-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ESRP1 at approximately 76 kDa. The expected band size for ESRP1 is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06068-1-esrp1-primary-antibodies-ihc-testing-1.jpgAnti-ESRP1 AntibodyIHC analysis of ESRP1 using anti-ESRP1 antibody (A06068-1). ESRP1 was detected in a paraffin-embedded section of human bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-ESRP1 Antibody (A06068-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06068-1-esrp1-primary-antibodies-ihc-testing-2.jpgAnti-ESRP1 AntibodyIHC analysis of ESRP1 using anti-ESRP1 antibody (A06068-1). ESRP1 was detected in a paraffin-embedded section of human lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-ESRP1 Antibody (A06068-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06068-1-esrp1-primary-antibodies-ihc-testing-3.jpgAnti-ESRP1 AntibodyIHC analysis of ESRP1 using anti-ESRP1 antibody (A06068-1). ESRP1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-ESRP1 Antibody (A06068-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06068-1-esrp1-primary-antibodies-ihc-testing-4.jpgAnti-ESRP1 AntibodyIHC analysis of ESRP1 using anti-ESRP1 antibody (A06068-1). ESRP1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-ESRP1 Antibody (A06068-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06068-1-esrp1-primary-antibodies-ihc-testing-5.jpgAnti-ESRP1 AntibodyIHC analysis of ESRP1 using anti-ESRP1 antibody (A06068-1). ESRP1 was detected in a paraffin-embedded section of human thyroid tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-ESRP1 Antibody (A06068-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06068-1-esrp1-primary-antibodies-ihc-testing-6.jpgAnti-ESRP1 AntibodyIHC analysis of ESRP1 using anti-ESRP1 antibody (A06068-1). ESRP1 was detected in a paraffin-embedded section of human breast tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-ESRP1 Antibody (A06068-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06068-1-esrp1-primary-antibodies-ihc-testing-7.jpgAnti-ESRP1 AntibodyIHC analysis of ESRP1 using anti-ESRP1 antibody (A06068-1). ESRP1 was detected in a paraffin-embedded section of human renal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-ESRP1 Antibody (A06068-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06068-1-esrp1-primary-antibodies-ihc-testing-8.jpgAnti-ESRP1 AntibodyIHC analysis of ESRP1 using anti-ESRP1 antibody (A06068-1). ESRP1 was detected in a paraffin-embedded section of human cerivial tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-ESRP1 Antibody (A06068-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06068-1-esrp1-primary-antibodies-ihc-testing-9.jpgAnti-ESRP1 AntibodyIHC analysis of ESRP1 using anti-ESRP1 antibody (A06068-1). ESRP1 was detected in a paraffin-embedded section of human overian tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-ESRP1 Antibody (A06068-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1296422026-04-02T05:01:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02646-4-fabp7-primary-antibodies-wb-testing-1.jpgAnti-FABP7 Antibody Picoband®Western blot analysis of FABP7 using anti-FABP7 antibody (A02646-4). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP7 antigen affinity purified polyclonal antibody (A02646-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FABP7 at approximately 15 kDa. The expected band size for FABP7 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02646-4-fabp7-primary-antibodies-ihc-testing-1.jpgAnti-FABP7 Antibody Picoband®IHC analysis of FABP7 using anti-FABP7 antibody (A02646-4). FABP7 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FABP7 Antibody (A02646-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1296432026-03-25T05:25:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296442026-03-16T05:08:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296452026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296462026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296472026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296482026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296492026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296502026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296512026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296522026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07247-1-pdcd2-primary-antibodies-wb-testing-1.jpgAnti-PDCD2 Antibody Picoband®Western blot analysis of PDCD2 using anti-PDCD2 antibody (A07247-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCD2 antigen affinity purified polyclonal antibody (A07247-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDCD2 at approximately 39,45 kDa. The expected band size for PDCD2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07247-1-pdcd2-primary-antibodies-if-testing-1.jpgAnti-PDCD2 Antibody Picoband®IF analysis of PDCD2 using anti-PDCD2 antibody (A07247-1) and anti-Tubulin Alpha antibody (M03989-3). PDCD2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PDCD2 Antibody (A07247-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07247-1-pdcd2-primary-antibodies-ip-testing-1.jpgAnti-PDCD2 Antibody Picoband®Immunoprecipitating (IP) PDCD2 in THP-1 whole cell lysate. Western blot analysis of PDCD2 using anti-PDCD2 antibody (A07247-1); Lane 1: THP-1 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-PDCD2 antibody in THP-1 whole cell lysate; Lane 3: anti-PDCD2 antibody (2μg) + THP-1 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PDCD2 antigen affinity purified polyclonal antibody (A07247-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PDCD2 at approximately 45 kDa. The expected band size for PDCD2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07247-1-pdcd2-primary-antibodies-fcm-testing-1.jpgAnti-PDCD2 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-PDCD2 antibody (A07247-1). Overlay histogram showing K562 cells stained with A07247-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDCD2 Antibody (A07247-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1296532026-03-17T05:16:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01875-2-pdgfd-primary-antibodies-wb-testing-1.jpgAnti-PDGFD Antibody Picoband®Western blot analysis of PDGFD using anti-PDGFD antibody (A01875-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFD antigen affinity purified polyclonal antibody (A01875-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDGFD at approximately 43 kDa. The expected band size for PDGFD is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01875-2-pdgfd-primary-antibodies-ihc-testing-1.jpgAnti-PDGFD Antibody Picoband®IHC analysis of PDGFD using anti-PDGFD antibody (A01875-2). PDGFD was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDGFD Antibody (A01875-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01875-2-pdgfd-primary-antibodies-ihc-testing-2.jpgAnti-PDGFD Antibody Picoband®IHC analysis of PDGFD using anti-PDGFD antibody (A01875-2). PDGFD was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDGFD Antibody (A01875-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01875-2-pdgfd-primary-antibodies-ihc-testing-3.jpgAnti-PDGFD Antibody Picoband®IHC analysis of PDGFD using anti-PDGFD antibody (A01875-2). PDGFD was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDGFD Antibody (A01875-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01875-2-pdgfd-primary-antibodies-if-testing-1.jpgAnti-PDGFD Antibody Picoband®IF analysis of PDGFD using anti-PDGFD antibody (A01875-2). PDGFD was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PDGFD Antibody (A01875-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1296542026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296562026-03-24T05:36:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05823-1-phlda1-primary-antibodies-wb-testing-1.jpgAnti-PHLDA1 AntibodyWestern blot analysis of PHLDA1 using anti-PHLDA1 antibody (A05823-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHLDA1 antigen affinity purified polyclonal antibody (A05823-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHLDA1 at approximately 40 kDa. The expected band size for PHLDA1 is at 45 kDa. https://www.bosterbio.com/catalog/product/view/id/1296572026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296582026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296592026-03-13T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01714-2-rac2-primary-antibodies-wb-testing-1.jpgAnti-RAC2 AntibodyWestern blot analysis of RAC2 using anti-RAC2 antibody (A01714-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAC2 antigen affinity purified polyclonal antibody (A01714-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAC2 at approximately 21 kDa. The expected band size for RAC2 is at 21 kDa. https://www.bosterbio.com/catalog/product/view/id/1296602026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296612026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296622026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296632026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296642026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296652026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296662026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296672026-03-16T05:08:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296682026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296692026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296702026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296712026-04-02T05:01:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06816-1-stk25-primary-antibodies-wb-testing-1.jpgAnti-STK25 Antibody Picoband®Western blot analysis of STK25 using anti-STK25 antibody (A06816-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STK25 antigen affinity purified polyclonal antibody (A06816-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STK25 at approximately 48 kDa. The expected band size for STK25 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06816-1-stk25-primary-antibodies-fcm-testing-1.jpgAnti-STK25 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-STK25 antibody (A06816-1). Overlay histogram showing U251 cells stained with A06816-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STK25 Antibody (A06816-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1296722026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296732026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296742026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296752026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05548-1-tfcp2-primary-antibodies-wb-testing-1.jpgAnti-TFCP2 Antibody Picoband®Western blot analysis of TFCP2 using anti-TFCP2 antibody (A05548-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFCP2 antigen affinity purified polyclonal antibody (A05548-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TFCP2 at approximately 65 kDa. The expected band size for TFCP2 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05548-1-tfcp2-primary-antibodies-fcm-testing-1.jpgAnti-TFCP2 Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-TFCP2 antibody (A05548-1). Overlay histogram showing Caco-2 cells stained with A05548-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFCP2 Antibody (A05548-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1296762026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296772026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10885-1-tmod4-primary-antibodies-wb-testing-1.jpgAnti-TMOD4 Antibody Picoband®Western blot analysis of TMOD4 using anti-TMOD4 antibody (A10885-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMOD4 antigen affinity purified polyclonal antibody (A10885-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMOD4 at approximately 39 kDa. The expected band size for TMOD4 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10885-1-tmod4-primary-antibodies-fcm-testing-1.jpgAnti-TMOD4 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-TMOD4 antibody (A10885-1). Overlay histogram showing HEL cells stained with A10885-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMOD4 Antibody (A10885-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1296792026-03-13T05:05:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296802026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296812026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296822026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296832026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296842026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296852026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296862026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296872026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296882026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296892026-03-16T05:08:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10380-1-atp6v1c1-primary-antibodies-wb-testing-1.jpgAnti-ATP6V1C1 Antibody Western blot analysis of ATP6V1C1 using anti-ATP6V1C1 antibody (A10380-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1C1 antigen affinity purified polyclonal antibody (A10380-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATP6V1C1 at approximately 42 kDa. The expected band size for ATP6V1C1 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10380-1-atp6v1c1-primary-antibodies-ihc-testing-1.jpgAnti-ATP6V1C1 AntibodyIHC analysis of ATP6V1C1 using anti-ATP6V1C1 antibody (A10380-1). ATP6V1C1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1C1 Antibody (A10380-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10380-1-atp6v1c1-primary-antibodies-ihc-testing-2.jpgAnti-ATP6V1C1 AntibodyIHC analysis of ATP6V1C1 using anti-ATP6V1C1 antibody (A10380-1). ATP6V1C1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP6V1C1 Antibody (A10380-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1296902026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296912026-03-16T05:08:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296922026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296932026-04-02T05:01:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11297-2-fbxw2-primary-antibodies-wb-testing-1.jpgAnti-FBXW2 Antibody Picoband®Western blot analysis of FBXW2 using anti-FBXW2 antibody (A11297-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXW2 antigen affinity purified polyclonal antibody (A11297-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXW2 at approximately 52 kDa. The expected band size for FBXW2 is at 52 kDa. https://www.bosterbio.com/catalog/product/view/id/1296942026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296952026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296962026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296972026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1296982026-03-16T05:08:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12497-3-klhl22-primary-antibodies-wb-testing-1.jpgAnti-KLHL22 AntibodyWestern blot analysis of KLHL22 using anti-KLHL22 antibody (A12497-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLHL22 antigen affinity purified polyclonal antibody (A12497-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KLHL22 at approximately 72 kDa. The expected band size for KLHL22 is at 72 kDa. https://www.bosterbio.com/catalog/product/view/id/1296992026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297002026-03-13T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04027-2-lyve1-primary-antibodies-wb-testing-1.jpgAnti-LYVE1 AntibodyWestern blot analysis of LYVE1 using anti-LYVE1 antibody (A04027-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYVE1 antigen affinity purified polyclonal antibody (A04027-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LYVE1 at approximately 70 kDa. The expected band size for LYVE1 is at 35 kDa. https://www.bosterbio.com/catalog/product/view/id/1297012026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297022026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297032026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02829-2-map4k4-primary-antibodies-wb-testing-1.jpgAnti-MAP4K4 Antibody Picoband®Western blot analysis of MAP4K4 using anti-MAP4K4 antibody (A02829-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K4 antigen affinity purified polyclonal antibody (A02829-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAP4K4 at approximately 160 kDa. The expected band size for MAP4K4 is at 142 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02829-2-map4k4-primary-antibodies-ihc-testing-1.jpgAnti-MAP4K4 Antibody Picoband®IHC analysis of MAP4K4 using anti-MAP4K4 antibody (A02829-2). MAP4K4 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAP4K4 Antibody (A02829-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02829-2-map4k4-primary-antibodies-ihc-testing-2.jpgAnti-MAP4K4 Antibody Picoband®IHC analysis of MAP4K4 using anti-MAP4K4 antibody (A02829-2). MAP4K4 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAP4K4 Antibody (A02829-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02829-2-map4k4-primary-antibodies-fcm-testing-1.jpgAnti-MAP4K4 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-MAP4K4 antibody (A02829-2). Overlay histogram showing Jurkat cells stained with A02829-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP4K4 Antibody (A02829-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1297042026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297052026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297062026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297072026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297082026-03-16T05:08:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12695-2-ngrn-primary-antibodies-wb-testing-1.jpgAnti-NGRN AntibodyWestern blot analysis of NGRN using anti-NGRN antibody (A12695-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NGRN antigen affinity purified polyclonal antibody (A12695-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NGRN at approximately 36 kDa. The expected band size for NGRN is at 32 kDa. https://www.bosterbio.com/catalog/product/view/id/1297092026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297102026-03-16T05:08:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12018-2-nptx1-primary-antibodies-wb-testing-1.jpgAnti-NPTX1 Antibody Western blot analysis of NPTX1 using anti-NPTX1 antibody (A12018-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPTX1 antigen affinity purified polyclonal antibody (A12018-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NPTX1 at approximately 47 kDa. The expected band size for NPTX1 is at 47 kDa. https://www.bosterbio.com/catalog/product/view/id/1297112026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297122026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297132026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297142026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297162026-03-16T05:08:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297172026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297182026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297192026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297202026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02711-1-plxnb1-primary-antibodies-wb-testing-1.jpgAnti-PLXNB1 Antibody Picoband®Western blot analysis of PLXNB1 using anti-PLXNB1 antibody (A02711-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLXNB1 antigen affinity purified polyclonal antibody (A02711-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLXNB1 at approximately 270 kDa. The expected band size for PLXNB1 is at 232 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02711-1-plxnb1-primary-antibodies-ihc-testing-1.jpgAnti-PLXNB1 Antibody Picoband®IHC analysis of PLXNB1 using anti-PLXNB1 antibody (A02711-1). PLXNB1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLXNB1 Antibody (A02711-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02711-1-plxnb1-primary-antibodies-ihc-testing-2.jpgAnti-PLXNB1 Antibody Picoband®IHC analysis of PLXNB1 using anti-PLXNB1 antibody (A02711-1). PLXNB1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLXNB1 Antibody (A02711-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02711-1-plxnb1-primary-antibodies-if-testing-1.jpgAnti-PLXNB1 Antibody Picoband®IF analysis of PLXNB1 using anti-PLXNB1 antibody (A02711-1). PLXNB1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PLXNB1 Antibody (A02711-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02711-1-plxnb1-primary-antibodies-if-testing-2.jpgAnti-PLXNB1 Antibody Picoband®IF analysis of PLXNB1 using anti-PLXNB1 antibody (A02711-1). PLXNB1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PLXNB1 Antibody (A02711-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02711-1-plxnb1-primary-antibodies-fcm-testing-1.jpgAnti-PLXNB1 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-PLXNB1 antibody (A02711-1). Overlay histogram showing RT4 cells stained with A02711-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLXNB1 Antibody (A02711-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1297212026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297222026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297232026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297242026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297252026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297262026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02188-3-psmb8-primary-antibodies-wb-testing-1.jpgAnti-PSMB8 Antibody Picoband®Western blot analysis of PSMB8 using anti-PSMB8 antibody (A02188-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB8 antigen affinity purified polyclonal antibody (A02188-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMB8 at approximately 23 kDa. The expected band size for PSMB8 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02188-3-psmb8-primary-antibodies-if-testing-1.jpgAnti-PSMB8 Antibody Picoband®IF analysis of PSMB8 using anti-PSMB8 antibody (A02188-3). PSMB8 was detected in a paraffin-embedded section of U2OS tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMB8 Antibody (A02188-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02188-3-psmb8-primary-antibodies-fcm-testing-1.jpgAnti-PSMB8 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-PSMB8 antibody (A02188-3). Overlay histogram showing Jurkat cells stained with A02188-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMB8 Antibody (A02188-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1297272026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09249-2-psmc6-primary-antibodies-wb-testing-1.jpgAnti-PSMC6 Antibody Picoband®Western blot analysis of PSMC6 using anti-PSMC6 antibody (A09249-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC6 antigen affinity purified polyclonal antibody (A09249-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMC6 at approximately 42 kDa. The expected band size for PSMC6 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09249-2-psmc6-primary-antibodies-if-testing-1.jpgAnti-PSMC6 Antibody Picoband®IF analysis of PSMC6 using anti-PSMC6 antibody (A09249-2) and anti-Alpha Tubulin antibody (M03989-3). PSMC6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMC6 Antibody (A09249-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09249-2-psmc6-primary-antibodies-ip-testing-1.jpgAnti-PSMC6 Antibody Picoband®Immunoprecipitating PSMC6 in Hela whole cell lysate. Western blot analysis of PSMC6 using anti-PSMC6 antibody (A09249-2). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-PSMC6 antibody in Hela whole cell lysate, Lane 3: anti-PSMC6 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PSMC6 antigen affinity purified polyclonal antibody (A09249-2) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PSMC6 at approximately 42 kDa. The expected band size for PSMC6 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09249-2-psmc6-primary-antibodies-fcm-testing-1.jpgAnti-PSMC6 Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-PSMC6 antibody (A09249-2). Overlay histogram showing HeLa cells stained with A09249-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMC6 Antibody (A09249-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1297282026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297292026-03-16T05:08:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10160-2-aars2-primary-antibodies-wb-testing-1.jpgAnti-AARS2 AntibodyWestern blot analysis of AARS2 using anti-AARS2 antibody (A10160-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AARS2 antigen affinity purified polyclonal antibody (A10160-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AARS2 at approximately 110 kDa. The expected band size for AARS2 is at 107 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10160-2-aars2-primary-antibodies-ihc-testing-1.jpgAnti-AARS2 AntibodyIHC analysis of AARS2 using anti-AARS2 antibody (A10160-2). AARS2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-AARS2 Antibody (A10160-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10160-2-aars2-primary-antibodies-ihc-testing-2.jpgAnti-AARS2 AntibodyIHC analysis of AARS2 using anti-AARS2 antibody (A10160-2). AARS2 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-AARS2 Antibody (A10160-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10160-2-aars2-primary-antibodies-ihc-testing-3.jpgAnti-AARS2 AntibodyIHC analysis of AARS2 using anti-AARS2 antibody (A10160-2). AARS2 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-AARS2 Antibody (A10160-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10160-2-aars2-primary-antibodies-ihc-testing-4.jpgAnti-AARS2 AntibodyIHC analysis of AARS2 using anti-AARS2 antibody (A10160-2). AARS2 was detected in a paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-AARS2 Antibody (A10160-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1297302026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297312026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297322026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297332026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297342026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297352026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297362026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10305-1-trim23-primary-antibodies-wb-testing-1.jpgAnti-TRIM23 Antibody Picoband®Western blot analysis of TRIM23 using anti-TRIM23 antibody (A10305-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM23 antigen affinity purified polyclonal antibody (A10305-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIM23 at approximately 64 kDa. The expected band size for TRIM23 is at 64 kDa. https://www.bosterbio.com/catalog/product/view/id/1297372026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297382026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09149-1-trim39-primary-antibodies-wb-testing-1.jpgAnti-TRIM39 Antibody Picoband®Western blot analysis of TRIM39 using anti-TRIM39 antibody (A09149-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM39 antigen affinity purified polyclonal antibody (A09149-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIM39 at approximately 65 kDa. The expected band size for TRIM39 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09149-1-trim39-primary-antibodies-fcm-testing-1.jpgAnti-TRIM39 Antibody Picoband®Flow Cytometry analysis of CACO-2 cells using anti-TRIM39 antibody (A09149-1). Overlay histogram showing CACO-2 cells stained with A09149-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM39 Antibody (A09149-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09149-1-trim39-primary-antibodies-fcm-testing-2.jpgAnti-TRIM39 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-TRIM39 antibody (A09149-1). Overlay histogram showing Jurkat cells stained with A09149-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM39 Antibody (A09149-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1297392026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297402026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297412026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297422026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297432026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06732-1-pik3c2b-primary-antibodies-wb-testing-1.jpgAnti-PIK3C2B Antibody Picoband®Western blot analysis of PIK3C2B using anti-PIK3C2B antibody (A06732-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3C2B antigen affinity purified polyclonal antibody (A06732-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIK3C2B at approximately 160-180 kDa. The expected band size for PIK3C2B is at 185 kDa. https://www.bosterbio.com/catalog/product/view/id/1297442026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297452026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297462026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297472026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297482026-03-13T05:05:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297492026-03-13T05:05:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1297502026-03-13T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02107-2-ly96-primary-antibodies-wb-testing-1.jpgAnti-MD2/LY96 AntibodyWestern blot analysis of MD2/LY96 using anti-MD2/LY96 antibody (A03575-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat tesis tissue lysates, Lane 2: mouse tesis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MD2/LY96 antigen affinity purified polyclonal antibody (A03575-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MD2/LY96 at approximately 26 kDa. The expected band size for MD2/LY96 is at 18 kDa. https://www.bosterbio.com/catalog/product/view/id/1297512026-04-02T05:01:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04499-2-rpl22-primary-antibodies-wb-testing-1.jpgAnti-RPL22 Antibody Picoband®Western blot analysis of RPL22 using anti-RPL22 antibody (A04499-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL22 antigen affinity purified polyclonal antibody (A04499-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RPL22 at approximately 15 kDa. The expected band size for RPL22 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04499-2-rpl22-primary-antibodies-ihc-testing-1.jpgAnti-RPL22 Antibody Picoband®IHC analysis of RPL22 using anti-RPL22 antibody (A04499-2). RPL22 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL22 Antibody (A04499-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04499-2-rpl22-primary-antibodies-ihc-testing-2.jpgAnti-RPL22 Antibody Picoband®IHC analysis of RPL22 using anti-RPL22 antibody (A04499-2). RPL22 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL22 Antibody (A04499-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04499-2-rpl22-primary-antibodies-if-testing-1.jpgAnti-RPL22 Antibody Picoband®IF analysis of RPL22 using anti-RPL22 antibody (A04499-2) and anti-Alpha Tubulin antibody (M03989-3). RPL22 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPL22 Antibody (A04499-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1297522026-03-16T05:08:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-p53-tp53-antibody-azp79734-boster.html2026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp79734-p53-tp53-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish P53/TP53 Antibody Picoband® Western blot analysis of P53/TP53 using anti-P53/TP53 antibody (AZP79734). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P53/TP53 antigen affinity purified polyclonal antibody (AZP79734) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P53/TP53 at approximately 53 kDa. The expected band size for P53/TP53 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp79734-p53-tp53-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish P53/TP53 Antibody Picoband® IHC analysis of P53/TP53 using anti-P53/TP53 antibody (AZP79734). P53/TP53 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P53/TP53 Antibody (AZP79734) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-myod1-antibody-azq90477-boster.html2026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90477-myod1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MYOD1 Antibody Picoband® Western blot analysis of MYOD1 using anti-MYOD1 antibody (AZQ90477). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYOD1 antigen affinity purified polyclonal antibody (AZQ90477) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYOD1 at approximately 40 kDa. The expected band size for MYOD1 is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-shha-antibody-azq92008-boster.html2026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq92008-shha-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SHHA Antibody Picoband® Western blot analysis of SHHA using anti-SHHA antibody (AZQ92008). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHHA antigen affinity purified polyclonal antibody (AZQ92008) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SHHA at approximately 55 kDa. The expected band size for SHHA is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq92008-shha-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish SHHA Antibody Picoband® IHC analysis of SHHA using anti-SHHA antibody (AZQ92008). SHHA was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHHA Antibody (AZQ92008) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq92008-shha-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish SHHA Antibody Picoband® IHC analysis of SHHA using anti-SHHA antibody (AZQ92008). SHHA was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHHA Antibody (AZQ92008) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq92008-shha-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish SHHA Antibody Picoband® IHC analysis of SHHA using anti-SHHA antibody (AZQ92008). SHHA was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHHA Antibody (AZQ92008) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq92008-shha-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish SHHA Antibody Picoband® IHC analysis of SHHA using anti-SHHA antibody (AZQ92008). SHHA was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHHA Antibody (AZQ92008) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq92008-shha-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish SHHA Antibody Picoband® IHC analysis of SHHA using anti-SHHA antibody (AZQ92008). SHHA was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHHA Antibody (AZQ92008) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-bmp2b-antibody-azo93369-boster.html2026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo93369-bmp2b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish BMP2B Antibody Picoband® Western blot analysis of BMP2B using anti-BMP2B antibody (AZO93369). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP2B antigen affinity purified polyclonal antibody (AZO93369) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BMP2B at approximately 47 kDa. The expected band size for BMP2B is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo93369-bmp2b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish BMP2B Antibody Picoband® IHC analysis of BMP2B using anti-BMP2B antibody (AZO93369). BMP2B was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BMP2B Antibody (AZO93369) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo93369-bmp2b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish BMP2B Antibody Picoband® IHC analysis of BMP2B using anti-BMP2B antibody (AZO93369). BMP2B was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BMP2B Antibody (AZO93369) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo93369-bmp2b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish BMP2B Antibody Picoband® IHC analysis of BMP2B using anti-BMP2B antibody (AZO93369). BMP2B was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BMP2B Antibody (AZO93369) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo93369-bmp2b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish BMP2B Antibody Picoband® IHC analysis of BMP2B using anti-BMP2B antibody (AZO93369). BMP2B was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BMP2B Antibody (AZO93369) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-spi1b-antibody-aza0a8m2bg46-boster.html2026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bg46-spi1b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SPI1B Antibody Picoband® Western blot analysis of SPI1B using anti-SPI1B antibody (AZA0A8M2BG46). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPI1B antigen affinity purified polyclonal antibody (AZA0A8M2BG46) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPI1B at approximately 35 kDa. The expected band size for SPI1B is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-c-myb-myb-antibody-azf1qp25-boster.html2026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qp25-c-myb-myb-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish c-Myb/MYB Antibody Picoband® Western blot analysis of c-Myb/MYB using anti-c-Myb/MYB antibody (AZF1QP25). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Myb/MYB antigen affinity purified polyclonal antibody (AZF1QP25) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for c-Myb/MYB at approximately 75 kDa. The expected band size for c-Myb/MYB is at 75 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-chordin-chrd-antibody-azo57472-boster.html2026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo57472-chordin-chrd-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Chordin/CHRD Antibody Picoband® Western blot analysis of Chordin/CHRD using anti-Chordin/CHRD antibody (AZO57472). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chordin/CHRD antigen affinity purified polyclonal antibody (AZO57472) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Chordin/CHRD at approximately 120 kDa. The expected band size for Chordin/CHRD is at 104 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fli1-antibody-aza0a8m9plw9-boster.html2026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9plw9-fli1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FLI1 Antibody Picoband® Western blot analysis of FLI1 using anti-FLI1 antibody (AZA0A8M9PLW9). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLI1 antigen affinity purified polyclonal antibody (AZA0A8M9PLW9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FLI1 at approximately 53 kDa. The expected band size for FLI1 is at 53 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mmp9-antibody-azq7t317-boster.html2026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t317-mmp9-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish MMP9 Antibody IHC analysis of MMP9 using anti-MMP9 antibody (AZQ7T317). MMP9 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP9 Antibody (AZQ7T317) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t317-mmp9-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish MMP9 Antibody IHC analysis of MMP9 using anti-MMP9 antibody (AZQ7T317). MMP9 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP9 Antibody (AZQ7T317) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t317-mmp9-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish MMP9 Antibody IHC analysis of MMP9 using anti-MMP9 antibody (AZQ7T317). MMP9 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP9 Antibody (AZQ7T317) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ndr2-antibody-azp87358-boster.html2026-03-17T05:16:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp87358-ndr2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NDR2 Antibody Picoband® Western blot analysis of NDR2 using anti-NDR2 antibody (AZP87358). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDR2 antigen affinity purified polyclonal antibody (AZP87358) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NDR2 at approximately 40 kDa. The expected band size for NDR2 is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gfap-antibody-azq58ee9-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq58ee9-gfap-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GFAP Antibody Picoband® Western blot analysis of GFAP using anti-GFAP antibody (AZQ58EE9). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFAP antigen affinity purified polyclonal antibody (AZQ58EE9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GFAP at approximately 51 kDa. The expected band size for GFAP is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cyp19a1b-antibody-azq5u870-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u870-cyp19a1b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CYP19A1B Antibody Picoband® Western blot analysis of CYP19A1B using anti-CYP19A1B antibody (AZQ5U870). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP19A1B antigen affinity purified polyclonal antibody (AZQ5U870) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CYP19A1B at approximately 58 kDa. The expected band size for CYP19A1B is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u870-cyp19a1b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CYP19A1B Antibody Picoband® IHC analysis of CYP19A1B using anti-CYP19A1B antibody (AZQ5U870). CYP19A1B was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP19A1B Antibody (AZQ5U870) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u870-cyp19a1b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish CYP19A1B Antibody Picoband® IHC analysis of CYP19A1B using anti-CYP19A1B antibody (AZQ5U870). CYP19A1B was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP19A1B Antibody (AZQ5U870) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u870-cyp19a1b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish CYP19A1B Antibody Picoband® IHC analysis of CYP19A1B using anti-CYP19A1B antibody (AZQ5U870). CYP19A1B was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP19A1B Antibody (AZQ5U870) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u870-cyp19a1b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish CYP19A1B Antibody Picoband® IHC analysis of CYP19A1B using anti-CYP19A1B antibody (AZQ5U870). CYP19A1B was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP19A1B Antibody (AZQ5U870) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u870-cyp19a1b-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish CYP19A1B Antibody Picoband® IHC analysis of CYP19A1B using anti-CYP19A1B antibody (AZQ5U870). CYP19A1B was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP19A1B Antibody (AZQ5U870) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mib1-antibody-azq804s5-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq804s5-mib1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MIB1 Antibody Picoband® Western blot analysis of MIB1 using anti-MIB1 antibody (AZQ804S5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIB1 antigen affinity purified polyclonal antibody (AZQ804S5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MIB1 at approximately 112 kDa. The expected band size for MIB1 is at 112 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sox32-antibody-azq90z46-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90z46-sox32-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SOX32 Antibody Picoband® Western blot analysis of SOX32 using anti-SOX32 antibody (AZQ90Z46). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX32 antigen affinity purified polyclonal antibody (AZQ90Z46) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SOX32 at approximately 35 kDa. The expected band size for SOX32 is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-esr2b-antibody-azb8jjl3-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jjl3-esr2b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ESR2B Antibody Picoband® Western blot analysis of ESR2B using anti-ESR2B antibody (AZB8JJL3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2B antigen affinity purified polyclonal antibody (AZB8JJL3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ESR2B at approximately 66 kDa. The expected band size for ESR2B is at 66 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-m-csfr-csf1r-antibody-azq9i8n6-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i8n6-m-csfr-csf1r-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish M-CSFR/CSF1R Antibody IHC analysis of M-CSFR/CSF1R using anti-M-CSFR/CSF1R antibody (AZQ9I8N6). M-CSFR/CSF1R was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-M-CSFR/CSF1R Antibody (AZQ9I8N6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i8n6-m-csfr-csf1r-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish M-CSFR/CSF1R Antibody IHC analysis of M-CSFR/CSF1R using anti-M-CSFR/CSF1R antibody (AZQ9I8N6). M-CSFR/CSF1R was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-M-CSFR/CSF1R Antibody (AZQ9I8N6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i8n6-m-csfr-csf1r-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish M-CSFR/CSF1R Antibody IHC analysis of M-CSFR/CSF1R using anti-M-CSFR/CSF1R antibody (AZQ9I8N6). M-CSFR/CSF1R was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-M-CSFR/CSF1R Antibody (AZQ9I8N6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lft1-antibody-azq9pw55-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pw55-lft1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LFT1 Antibody Picoband® Western blot analysis of LFT1 using anti-LFT1 antibody (AZQ9PW55). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LFT1 antigen affinity purified polyclonal antibody (AZQ9PW55) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LFT1 at approximately 38 kDa. The expected band size for LFT1 is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ndr1-antibody-azo13144-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo13144-ndr1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish NDR1 Antibody IHC analysis of NDR1 using anti-NDR1 antibody (AZO13144). NDR1 was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDR1 Antibody (AZO13144) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo13144-ndr1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish NDR1 Antibody IHC analysis of NDR1 using anti-NDR1 antibody (AZO13144). NDR1 was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDR1 Antibody (AZO13144) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo13144-ndr1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish NDR1 Antibody IHC analysis of NDR1 using anti-NDR1 antibody (AZO13144). NDR1 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDR1 Antibody (AZO13144) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-npas4l-antibody-azp0doc7-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp0doc7-npas4l-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NPAS4L Antibody Picoband® Western blot analysis of NPAS4L using anti-NPAS4L antibody (AZP0DOC7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPAS4L antigen affinity purified polyclonal antibody (AZP0DOC7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NPAS4L at approximately 90 kDa. The expected band size for NPAS4L is at 69 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-glucocorticoid-receptor-nr3c1-antibody-azq1xhk0-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1xhk0-glucocorticoid-receptor-nr3c1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Glucocorticoid receptor/NR3C1 Antibody Picoband® Western blot analysis of Glucocorticoid receptor/NR3C1 using anti-Glucocorticoid receptor/NR3C1 antibody (AZQ1XHK0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glucocorticoid receptor/NR3C1 antigen affinity purified polyclonal antibody (AZQ1XHK0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Glucocorticoid receptor/NR3C1 at approximately 94 kDa. The expected band size for Glucocorticoid receptor/NR3C1 is at 82 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ppar-gamma-pparg-antibody-aza6xmh6-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza6xmh6-ppar-gamma-pparg-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PPAR Gamma/PPARG Antibody Picoband® Western blot analysis of PPAR Gamma/PPARG using anti-PPAR Gamma/PPARG antibody (AZA6XMH6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPAR Gamma/PPARG antigen affinity purified polyclonal antibody (AZA6XMH6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPAR Gamma/PPARG at approximately 60 kDa. The expected band size for PPAR Gamma/PPARG is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-n-cadherin-cdh2-antibody-azq90275-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90275-n-cadherin-cdh2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish N-Cadherin/CDH2 Antibody Picoband® Western blot analysis of N-Cadherin/CDH2 using anti-N-Cadherin/CDH2 antibody (AZQ90275). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-N-Cadherin/CDH2 antigen affinity purified polyclonal antibody (AZQ90275) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for N-Cadherin/CDH2 at approximately 130 kDa. The expected band size for N-Cadherin/CDH2 is at 98 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-caspase-9-casp9-antibody-azf1qsb1-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qsb1-caspase-9-casp9-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Caspase 9/CASP9 Antibody Picoband® Western blot analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (AZF1QSB1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase 9/CASP9 antigen affinity purified polyclonal antibody (AZF1QSB1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Caspase 9/CASP9 at approximately 50 kDa. The expected band size for Caspase 9/CASP9 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qsb1-caspase-9-casp9-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Caspase 9/CASP9 Antibody Picoband® IHC analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (AZF1QSB1). Caspase 9/CASP9 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caspase 9/CASP9 Antibody (AZF1QSB1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qsb1-caspase-9-casp9-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish Caspase 9/CASP9 Antibody Picoband® IHC analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (AZF1QSB1). Caspase 9/CASP9 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caspase 9/CASP9 Antibody (AZF1QSB1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qsb1-caspase-9-casp9-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish Caspase 9/CASP9 Antibody Picoband® IHC analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (AZF1QSB1). Caspase 9/CASP9 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caspase 9/CASP9 Antibody (AZF1QSB1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qsb1-caspase-9-casp9-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish Caspase 9/CASP9 Antibody Picoband® IHC analysis of Caspase 9/CASP9 using anti-Caspase 9/CASP9 antibody (AZF1QSB1). Caspase 9/CASP9 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caspase 9/CASP9 Antibody (AZF1QSB1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-il-1-beta-il1b-antibody-aza0a8m1pi54-boster.html2026-04-06T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1pi54-il-1-beta-il1b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish IL-1 beta/IL1B Antibody IHC analysis of IL-1 beta/IL1B using anti-IL-1 beta/IL1B antibody (AZA0A8M1PI54). IL-1 beta/IL1B was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL-1 beta/IL1B Antibody (AZA0A8M1PI54) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1pi54-il-1-beta-il1b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish IL-1 beta/IL1B Antibody IHC analysis of IL-1 beta/IL1B using anti-IL-1 beta/IL1B antibody (AZA0A8M1PI54). IL-1 beta/IL1B was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL-1 beta/IL1B Antibody (AZA0A8M1PI54) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1pi54-il-1-beta-il1b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish IL-1 beta/IL1B Antibody IHC analysis of IL-1 beta/IL1B using anti-IL-1 beta/IL1B antibody (AZA0A8M1PI54). IL-1 beta/IL1B was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL-1 beta/IL1B Antibody (AZA0A8M1PI54) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1pi54-il-1-beta-il1b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish IL-1 beta/IL1B Antibody IHC analysis of IL-1 beta/IL1B using anti-IL-1 beta/IL1B antibody (AZA0A8M1PI54). IL-1 beta/IL1B was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL-1 beta/IL1B Antibody (AZA0A8M1PI54) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1pi54-il-1-beta-il1b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish IL-1 beta/IL1B Antibody IHC analysis of IL-1 beta/IL1B using anti-IL-1 beta/IL1B antibody (AZA0A8M1PI54). IL-1 beta/IL1B was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL-1 beta/IL1B Antibody (AZA0A8M1PI54) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tnfa-antibody-azq08cq3-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq08cq3-tnfa-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TNFA Antibody Picoband® Western blot analysis of TNFA using anti-TNFA antibody (AZQ08CQ3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFA antigen affinity purified polyclonal antibody (AZQ08CQ3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNFA at approximately 26 kDa. The expected band size for TNFA is at 25 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dlx2a-antibody-azp50574-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp50574-dlx2a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DLX2A Antibody Picoband® Western blot analysis of DLX2A using anti-DLX2A antibody (AZP50574). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLX2A antigen affinity purified polyclonal antibody (AZP50574) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DLX2A at approximately 29 kDa. The expected band size for DLX2A is at 29 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sod1-antibody-azo73872-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo73872-sod1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SOD1 Antibody Picoband® Western blot analysis of SOD1 using anti-SOD1 antibody (AZO73872). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD1 antigen affinity purified polyclonal antibody (AZO73872) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SOD1 at approximately 16 kDa. The expected band size for SOD1 is at 16 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cxcl8a-antibody-aza0a0g2kyh9-boster.html2026-03-17T05:16:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0g2kyh9-cxcl8a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish CXCL8A Antibody IHC analysis of CXCL8A using anti-CXCL8A antibody (AZA0A0G2KYH9). CXCL8A was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL8A Antibody (AZA0A0G2KYH9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0g2kyh9-cxcl8a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CXCL8A Antibody IHC analysis of CXCL8A using anti-CXCL8A antibody (AZA0A0G2KYH9). CXCL8A was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL8A Antibody (AZA0A0G2KYH9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0g2kyh9-cxcl8a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish CXCL8A Antibody IHC analysis of CXCL8A using anti-CXCL8A antibody (AZA0A0G2KYH9). CXCL8A was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL8A Antibody (AZA0A0G2KYH9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0g2kyh9-cxcl8a-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish CXCL8A Antibody IHC analysis of CXCL8A using anti-CXCL8A antibody (AZA0A0G2KYH9). CXCL8A was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL8A Antibody (AZA0A0G2KYH9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0g2kyh9-cxcl8a-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish CXCL8A Antibody IHC analysis of CXCL8A using anti-CXCL8A antibody (AZA0A0G2KYH9). CXCL8A was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL8A Antibody (AZA0A0G2KYH9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-bcl2a-antibody-azq564a4-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq564a4-bcl2a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish BCL2A Antibody Picoband® Western blot analysis of BCL2A using anti-BCL2A antibody (AZQ564A4). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2A antigen affinity purified polyclonal antibody (AZQ564A4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BCL2A at approximately 26 kDa. The expected band size for BCL2A is at 26 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lysozyme-lyz-antibody-azq90ys5-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90ys5-lysozyme-lyz-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Lysozyme/LYZ Antibody Picoband® Western blot analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (AZQ90YS5). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lysozyme/LYZ antigen affinity purified polyclonal antibody (AZQ90YS5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Lysozyme/LYZ at approximately 15 kDa. The expected band size for Lysozyme/LYZ is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90ys5-lysozyme-lyz-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Lysozyme/LYZ Antibody Picoband® IHC analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (AZQ90YS5). Lysozyme/LYZ was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lysozyme/LYZ Antibody (AZQ90YS5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90ys5-lysozyme-lyz-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish Lysozyme/LYZ Antibody Picoband® IHC analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (AZQ90YS5). Lysozyme/LYZ was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lysozyme/LYZ Antibody (AZQ90YS5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-noto-antibody-azq90461-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90461-noto-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NOTO Antibody Picoband® Western blot analysis of NOTO using anti-NOTO antibody (AZQ90461). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOTO antigen affinity purified polyclonal antibody (AZQ90461) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NOTO at approximately 27 kDa. The expected band size for NOTO is at 27 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-p21-cdkn1a-antibody-aza0a8m1rfs1-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1rfs1-p21-cdkn1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish p21/CDKN1A Antibody Picoband® Western blot analysis of p21/CDKN1A using anti-p21/CDKN1A antibody (AZA0A8M1RFS1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p21/CDKN1A antigen affinity purified polyclonal antibody (AZA0A8M1RFS1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for p21/CDKN1A at approximately 15 kDa. The expected band size for p21/CDKN1A is at 17 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sod2-antibody-azq6p980-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p980-sod2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SOD2 Antibody Picoband® Western blot analysis of SOD2 using anti-SOD2 antibody (AZQ6P980). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2 antigen affinity purified polyclonal antibody (AZQ6P980) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SOD2 at approximately 25 kDa. The expected band size for SOD2 is at 25 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hand2-antibody-azp57102-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp57102-hand2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HAND2 Antibody IHC analysis of HAND2 using anti-HAND2 antibody (AZP57102). HAND2 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HAND2 Antibody (AZP57102) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp57102-hand2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HAND2 Antibody IHC analysis of HAND2 using anti-HAND2 antibody (AZP57102). HAND2 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HAND2 Antibody (AZP57102) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pcna-antibody-azq9ptp1-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9ptp1-pcna-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PCNA Antibody Picoband® Western blot analysis of PCNA using anti-PCNA antibody (AZQ9PTP1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCNA antigen affinity purified polyclonal antibody (AZQ9PTP1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCNA at approximately 36 kDa. The expected band size for PCNA is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hhex-antibody-azq9iav3-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9iav3-hhex-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HHEX Antibody Picoband® Western blot analysis of HHEX using anti-HHEX antibody (AZQ9IAV3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HHEX antigen affinity purified polyclonal antibody (AZQ9IAV3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HHEX at approximately 26 kDa. The expected band size for HHEX is at 26 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tbx5a-antibody-azq9iak8-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9iak8-tbx5a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TBX5A Antibody Picoband® Western blot analysis of TBX5A using anti-TBX5A antibody (AZQ9IAK8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBX5A antigen affinity purified polyclonal antibody (AZQ9IAK8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TBX5A at approximately 55 kDa. The expected band size for TBX5A is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lmo2-antibody-azq9ptj3-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9ptj3-lmo2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LMO2 Antibody Picoband® Western blot analysis of LMO2 using anti-LMO2 antibody (AZQ9PTJ3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO2 antigen affinity purified polyclonal antibody (AZQ9PTJ3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LMO2 at approximately 20 kDa. The expected band size for LMO2 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9ptj3-lmo2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish LMO2 Antibody Picoband® IHC analysis of LMO2 using anti-LMO2 antibody (AZQ9PTJ3). LMO2 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LMO2 Antibody (AZQ9PTJ3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9ptj3-lmo2-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish LMO2 Antibody Picoband® IHC analysis of LMO2 using anti-LMO2 antibody (AZQ9PTJ3). LMO2 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LMO2 Antibody (AZQ9PTJ3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cyp17a1-antibody-azb3dh80-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb3dh80-cyp17a1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish CYP17A1 Antibody IHC analysis of CYP17A1 using anti-CYP17A1 antibody (AZB3DH80). CYP17A1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP17A1 Antibody (AZB3DH80) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb3dh80-cyp17a1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CYP17A1 Antibody IHC analysis of CYP17A1 using anti-CYP17A1 antibody (AZB3DH80). CYP17A1 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP17A1 Antibody (AZB3DH80) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-star-antibody-azq9dg10-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9dg10-star-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish STAR Antibody Picoband® Western blot analysis of STAR using anti-STAR antibody (AZQ9DG10). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAR antigen affinity purified polyclonal antibody (AZQ9DG10) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STAR at approximately 36 kDa. The expected band size for STAR is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fasn-antibody-aze7f5v3-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f5v3-fasn-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FASN Antibody Picoband® Western blot analysis of FASN using anti-FASN antibody (AZE7F5V3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FASN antigen affinity purified polyclonal antibody (AZE7F5V3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FASN at approximately 250 kDa. The expected band size for FASN is at 274 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f5v3-fasn-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish FASN Antibody Picoband® IHC analysis of FASN using anti-FASN antibody (AZE7F5V3). FASN was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FASN Antibody (AZE7F5V3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f5v3-fasn-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish FASN Antibody Picoband® IHC analysis of FASN using anti-FASN antibody (AZE7F5V3). FASN was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FASN Antibody (AZE7F5V3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f5v3-fasn-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish FASN Antibody Picoband® IHC analysis of FASN using anti-FASN antibody (AZE7F5V3). FASN was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FASN Antibody (AZE7F5V3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-atoh7-antibody-azq8aw52-boster.html2026-04-06T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8aw52-atoh7-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish ATOH7 Antibody IHC analysis of ATOH7 using anti-ATOH7 antibody (AZQ8AW52). ATOH7 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATOH7 Antibody (AZQ8AW52) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-myca-antibody-azp52160-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp52160-myca-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MYCA Antibody Picoband® Western blot analysis of MYCA using anti-MYCA antibody (AZP52160). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYCA antigen affinity purified polyclonal antibody (AZP52160) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYCA at approximately 48 kDa. The expected band size for MYCA is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nkx2-2a-antibody-azq90481-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90481-nkx2-2a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NKX2.2A Antibody Picoband® Western blot analysis of NKX2.2A using anti-NKX2.2A antibody (AZQ90481). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKX2.2A antigen affinity purified polyclonal antibody (AZQ90481) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NKX2.2A at approximately 36 kDa. The expected band size for NKX2.2A is at 30 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cyp26a1-antibody-azp79739-boster.html2026-03-17T05:16:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp79739-cyp26a1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CYP26A1 Antibody Picoband® Western blot analysis of CYP26A1 using anti-CYP26A1 antibody (AZP79739). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates. Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP26A1 antigen affinity purified polyclonal antibody (AZP79739) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CYP26A1 at approximately 56 kDa. The expected band size for CYP26A1 is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pou5f1-3-antibody-azq90270-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90270-pou5f1-3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish POU5F1/3 Antibody Picoband® Western blot analysis of POU5F1/3 using anti-POU5F1/3 antibody (AZQ90270). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POU5F1/3 antigen affinity purified polyclonal antibody (AZQ90270) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POU5F1/3 at approximately 43 kDa. The expected band size for POU5F1/3 is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cyp11a1-1-antibody-azq7syj6-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7syj6-cyp11a1-1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CYP11A1.1 Antibody Picoband® Western blot analysis of CYP11A1.1 using anti-CYP11A1.1 antibody (AZQ7SYJ6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP11A1.1 antigen affinity purified polyclonal antibody (AZQ7SYJ6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CYP11A1.1 at approximately 58 kDa. The expected band size for CYP11A1.1 is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-etsrp-antibody-aza0a2r8q5a3-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8q5a3-etsrp-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ETSRP Antibody Picoband® Western blot analysis of ETSRP using anti-ETSRP antibody (AZA0A2R8Q5A3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETSRP antigen affinity purified polyclonal antibody (AZA0A2R8Q5A3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ETSRP at approximately 39 kDa. The expected band size for ETSRP is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8q5a3-etsrp-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ETSRP Antibody Picoband® IHC analysis of ETSRP using anti-ETSRP antibody (AZA0A2R8Q5A3). ETSRP was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ETSRP Antibody (AZA0A2R8Q5A3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-myd88-antibody-azq5xj85-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5xj85-myd88-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MYD88 Antibody Picoband® Western blot analysis of MYD88 using anti-MYD88 antibody (AZQ5XJ85). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (AZQ5XJ85) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYD88 at approximately 33 kDa. The expected band size for MYD88 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5xj85-myd88-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish MYD88 Antibody Picoband® IHC analysis of MYD88 using anti-MYD88 antibody (AZQ5XJ85). MYD88 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYD88 Antibody (AZQ5XJ85) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5xj85-myd88-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish MYD88 Antibody Picoband® IHC analysis of MYD88 using anti-MYD88 antibody (AZQ5XJ85). MYD88 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYD88 Antibody (AZQ5XJ85) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5xj85-myd88-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish MYD88 Antibody Picoband® IHC analysis of MYD88 using anti-MYD88 antibody (AZQ5XJ85). MYD88 was detected in a paraffin-embedded section of zebrafish thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYD88 Antibody (AZQ5XJ85) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-alcama-antibody-azq90460-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90460-alcama-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ALCAMA Antibody Picoband® Western blot analysis of ALCAMA using anti-ALCAMA antibody (AZQ90460). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALCAMA antigen affinity purified polyclonal antibody (AZQ90460) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALCAMA at approximately 95 kDa. The expected band size for ALCAMA is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90460-alcama-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ALCAMA Antibody Picoband® IHC analysis of ALCAMA using anti-ALCAMA antibody (AZQ90460). ALCAMA was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALCAMA Antibody (AZQ90460) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90460-alcama-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish ALCAMA Antibody Picoband® IHC analysis of ALCAMA using anti-ALCAMA antibody (AZQ90460). ALCAMA was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALCAMA Antibody (AZQ90460) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90460-alcama-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish ALCAMA Antibody Picoband® IHC analysis of ALCAMA using anti-ALCAMA antibody (AZQ90460). ALCAMA was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALCAMA Antibody (AZQ90460) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90460-alcama-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish ALCAMA Antibody Picoband® IHC analysis of ALCAMA using anti-ALCAMA antibody (AZQ90460). ALCAMA was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALCAMA Antibody (AZQ90460) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90460-alcama-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish ALCAMA Antibody Picoband® IHC analysis of ALCAMA using anti-ALCAMA antibody (AZQ90460). ALCAMA was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALCAMA Antibody (AZQ90460) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90460-alcama-primary-antibodies-ihc-testing-7.jpgAnti-Zebrafish ALCAMA Antibody Picoband® IHC analysis of ALCAMA using anti-ALCAMA antibody (AZQ90460). ALCAMA was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALCAMA Antibody (AZQ90460) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nanos3-antibody-azq90ww1-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90ww1-nanos3-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish NANOS3 Antibody IHC analysis of NANOS3 using anti-NANOS3 antibody (AZQ90WW1). NANOS3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NANOS3 Antibody (AZQ90WW1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90ww1-nanos3-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish NANOS3 Antibody IHC analysis of NANOS3 using anti-NANOS3 antibody (AZQ90WW1). NANOS3 was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NANOS3 Antibody (AZQ90WW1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90ww1-nanos3-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish NANOS3 Antibody IHC analysis of NANOS3 using anti-NANOS3 antibody (AZQ90WW1). NANOS3 was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NANOS3 Antibody (AZQ90WW1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pdx1-antibody-azq6dc85-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dc85-pdx1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PDX1 Antibody Picoband® Western blot analysis of PDX1 using anti-PDX1 antibody (AZQ6DC85). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDX1 antigen affinity purified polyclonal antibody (AZQ6DC85) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDX1 at approximately 38 kDa. The expected band size for PDX1 is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-e-cadherin-cdh1-antibody-azq90z37-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90z37-e-cadherin-cdh1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish E-cadherin/CDH1 Antibody Picoband® Western blot analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (AZQ90Z37). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E-cadherin/CDH1 antigen affinity purified polyclonal antibody (AZQ90Z37) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for E-cadherin/CDH1 at approximately 130 kDa. The expected band size for E-cadherin/CDH1 is at 120 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90z37-e-cadherin-cdh1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish E-cadherin/CDH1 Antibody Picoband® IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (AZQ90Z37). E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish inner ear tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-E-cadherin/CDH1 Antibody (AZQ90Z37) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90z37-e-cadherin-cdh1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish E-cadherin/CDH1 Antibody Picoband® IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (AZQ90Z37). E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-E-cadherin/CDH1 Antibody (AZQ90Z37) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90z37-e-cadherin-cdh1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish E-cadherin/CDH1 Antibody Picoband® IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (AZQ90Z37). E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish esophageal epithelium tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-E-cadherin/CDH1 Antibody (AZQ90Z37) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90z37-e-cadherin-cdh1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish E-cadherin/CDH1 Antibody Picoband® IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (AZQ90Z37). E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-E-cadherin/CDH1 Antibody (AZQ90Z37) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90z37-e-cadherin-cdh1-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish E-cadherin/CDH1 Antibody Picoband® IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (AZQ90Z37). E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-E-cadherin/CDH1 Antibody (AZQ90Z37) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90z37-e-cadherin-cdh1-primary-antibodies-ihc-testing-7.jpgAnti-Zebrafish E-cadherin/CDH1 Antibody Picoband® IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (AZQ90Z37). E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-E-cadherin/CDH1 Antibody (AZQ90Z37) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90z37-e-cadherin-cdh1-primary-antibodies-ihc-testing-8.jpgAnti-Zebrafish E-cadherin/CDH1 Antibody Picoband® IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (AZQ90Z37). E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-E-cadherin/CDH1 Antibody (AZQ90Z37) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cxcl12a-b-antibody-azq8av10-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8av10-cxcl12a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish CXCL12A/B Antibody IHC analysis of CXCL12A/B using anti-CXCL12A/B antibody (AZQ8AV10). CXCL12A/B was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL12A/B Antibody (AZQ8AV10) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8av10-cxcl12a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CXCL12A/B Antibody IHC analysis of CXCL12A/B using anti-CXCL12A/B antibody (AZQ8AV10). CXCL12A/B was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL12A/B Antibody (AZQ8AV10) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8av10-cxcl12a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish CXCL12A/B Antibody IHC analysis of CXCL12A/B using anti-CXCL12A/B antibody (AZQ8AV10). CXCL12A/B was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL12A/B Antibody (AZQ8AV10) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8av10-cxcl12a-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish CXCL12A/B Antibody IHC analysis of CXCL12A/B using anti-CXCL12A/B antibody (AZQ8AV10). CXCL12A/B was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL12A/B Antibody (AZQ8AV10) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8av10-cxcl12a-b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish CXCL12A/B Antibody IHC analysis of CXCL12A/B using anti-CXCL12A/B antibody (AZQ8AV10). CXCL12A/B was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL12A/B Antibody (AZQ8AV10) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-androgen-receptor-ar-antibody-aza4gt83-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza4gt83-androgen-receptor-ar-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Androgen receptor/AR Antibody Picoband® Western blot analysis of Androgen receptor/AR using anti-Androgen receptor/AR antibody (AZA4GT83). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Androgen receptor/AR antigen affinity purified polyclonal antibody (AZA4GT83) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Androgen receptor/AR at approximately 96 kDa. The expected band size for Androgen receptor/AR is at 96 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mpeg1-antibody-azq7sxe0-boster.html2026-04-06T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxe0-mpeg1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish MPEG1 Antibody IHC analysis of MPEG1 using anti-MPEG1 antibody (AZQ7SXE0). MPEG1 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPEG1 Antibody (AZQ7SXE0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxe0-mpeg1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish MPEG1 Antibody IHC analysis of MPEG1 using anti-MPEG1 antibody (AZQ7SXE0). MPEG1 was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPEG1 Antibody (AZQ7SXE0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxe0-mpeg1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish MPEG1 Antibody IHC analysis of MPEG1 using anti-MPEG1 antibody (AZQ7SXE0). MPEG1 was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPEG1 Antibody (AZQ7SXE0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxe0-mpeg1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish MPEG1 Antibody IHC analysis of MPEG1 using anti-MPEG1 antibody (AZQ7SXE0). MPEG1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPEG1 Antibody (AZQ7SXE0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxe0-mpeg1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish MPEG1 Antibody IHC analysis of MPEG1 using anti-MPEG1 antibody (AZQ7SXE0). MPEG1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPEG1 Antibody (AZQ7SXE0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ascl1a-antibody-azq90259-1-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90259-1-ascl1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ASCL1A Antibody Picoband® Western blot analysis of ASCL1A using anti-ASCL1A antibody (AZQ90259-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASCL1A antigen affinity purified polyclonal antibody (AZQ90259-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ASCL1A at approximately 22 kDa. The expected band size for ASCL1A is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-axin2-antibody-azp57095-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp57095-axin2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish AXIN2 Antibody IHC analysis of AXIN2 using anti-AXIN2 antibody (AZP57095). AXIN2 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AXIN2 Antibody (AZP57095) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp57095-axin2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish AXIN2 Antibody IHC analysis of AXIN2 using anti-AXIN2 antibody (AZP57095). AXIN2 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AXIN2 Antibody (AZP57095) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-igf1-antibody-azq90vv9-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90vv9-igf1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish IGF1 Antibody IHC analysis of IGF1 using anti-IGF1 antibody (AZQ90VV9). IGF1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF1 Antibody (AZQ90VV9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90vv9-igf1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish IGF1 Antibody IHC analysis of IGF1 using anti-IGF1 antibody (AZQ90VV9). IGF1 was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF1 Antibody (AZQ90VV9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90vv9-igf1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish IGF1 Antibody IHC analysis of IGF1 using anti-IGF1 antibody (AZQ90VV9). IGF1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF1 Antibody (AZQ90VV9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90vv9-igf1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish IGF1 Antibody IHC analysis of IGF1 using anti-IGF1 antibody (AZQ90VV9). IGF1 was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF1 Antibody (AZQ90VV9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90vv9-igf1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish IGF1 Antibody IHC analysis of IGF1 using anti-IGF1 antibody (AZQ90VV9). IGF1 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF1 Antibody (AZQ90VV9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hbae1-1-antibody-azq7zt21-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zt21-hbae1-1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HBAE1.1 Antibody Picoband® Western blot analysis of HBAE1.1 using anti-HBAE1.1 antibody (AZQ7ZT21). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. Lane 2: whole female zebrafish tissue lysates. Lane 3: whole male zebrafish tissue lysates. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBAE1.1 antigen affinity purified polyclonal antibody (AZQ7ZT21) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HBAE1.1 at approximately 15 kDa. The expected band size for HBAE1.1 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zt21-hbae1-1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HBAE1.1 Antibody Picoband® IHC analysis of HBAE1.1 using anti-HBAE1.1 antibody (AZQ7ZT21). HBAE1.1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HBAE1.1 Antibody (AZQ7ZT21) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zt21-hbae1-1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HBAE1.1 Antibody Picoband® IHC analysis of HBAE1.1 using anti-HBAE1.1 antibody (AZQ7ZT21). HBAE1.1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HBAE1.1 Antibody (AZQ7ZT21) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-srebf1-antibody-aza6xld8-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza6xld8-srebf1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish SREBF1 Antibody IHC analysis of SREBF1 using anti-SREBF1 antibody (AZA6XLD8). SREBF1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SREBF1 Antibody (AZA6XLD8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza6xld8-srebf1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish SREBF1 Antibody IHC analysis of SREBF1 using anti-SREBF1 antibody (AZA6XLD8). SREBF1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SREBF1 Antibody (AZA6XLD8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hnrnpa1a-b-antibody-azq7sxq3-boster.html2026-03-17T05:16:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxq3-hnrnpa1a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HNRNPA1a/b Antibody IHC analysis of HNRNPA1a/b using anti-HNRNPA1a/b antibody (AZQ7SXQ3). HNRNPA1a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HNRNPA1a/b Antibody (AZQ7SXQ3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxq3-hnrnpa1a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HNRNPA1a/b Antibody IHC analysis of HNRNPA1a/b using anti-HNRNPA1a/b antibody (AZQ7SXQ3). HNRNPA1a/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HNRNPA1a/b Antibody (AZQ7SXQ3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxq3-hnrnpa1a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HNRNPA1a/b Antibody IHC analysis of HNRNPA1a/b using anti-HNRNPA1a/b antibody (AZQ7SXQ3). HNRNPA1a/b was detected in a paraffin-embedded section of zebrafish esophagus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HNRNPA1a/b Antibody (AZQ7SXQ3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hnrnpd-antibody-aza0a8m9qc46-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qc46-hnrnpd-primary-antibodies-if-testing-1.jpgAnti-Zebrafish HNRNPD Antibody Picoband® IF analysis of HNRNPD using anti-HNRNPD antibody (AZA0A8M9QC46). HNRNPD was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-HNRNPD Antibody (AZA0A8M9QC46) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qc46-hnrnpd-primary-antibodies-wb-testing-2.jpgAnti-Zebrafish HNRNPD Antibody Picoband® Western blot analysis of HNRNPD using anti-HNRNPD antibody (AZA0A8M9QC46). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNRNPD antigen affinity purified polyclonal antibody (AZA0A8M9QC46) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HNRNPD at approximately 40 kDa. The expected band size for HNRNPD is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qc46-hnrnpd-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HNRNPD Antibody Picoband® IHC analysis of HNRNPD using anti-HNRNPD antibody (AZA0A8M9QC46). HNRNPD was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HNRNPD Antibody (AZA0A8M9QC46) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qc46-hnrnpd-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish HNRNPD Antibody Picoband® IHC analysis of HNRNPD using anti-HNRNPD antibody (AZA0A8M9QC46). HNRNPD was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HNRNPD Antibody (AZA0A8M9QC46) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qc46-hnrnpd-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish HNRNPD Antibody Picoband® IHC analysis of HNRNPD using anti-HNRNPD antibody (AZA0A8M9QC46). HNRNPD was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HNRNPD Antibody (AZA0A8M9QC46) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qc46-hnrnpd-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish HNRNPD Antibody Picoband® IHC analysis of HNRNPD using anti-HNRNPD antibody (AZA0A8M9QC46). HNRNPD was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HNRNPD Antibody (AZA0A8M9QC46) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qc46-hnrnpd-primary-antibodies-ihc-testing-7.jpgAnti-Zebrafish HNRNPD Antibody Picoband® IHC analysis of HNRNPD using anti-HNRNPD antibody (AZA0A8M9QC46). HNRNPD was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HNRNPD Antibody (AZA0A8M9QC46) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hook3-antibody-aza0a8m6z0p5-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6z0p5-hook3-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HOOK3 Antibody IHC analysis of HOOK3 using anti-HOOK3 antibody (AZA0A8M6Z0P5). HOOK3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOOK3 Antibody (AZA0A8M6Z0P5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6z0p5-hook3-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HOOK3 Antibody IHC analysis of HOOK3 using anti-HOOK3 antibody (AZA0A8M6Z0P5). HOOK3 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOOK3 Antibody (AZA0A8M6Z0P5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6z0p5-hook3-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HOOK3 Antibody IHC analysis of HOOK3 using anti-HOOK3 antibody (AZA0A8M6Z0P5). HOOK3 was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOOK3 Antibody (AZA0A8M6Z0P5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6z0p5-hook3-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish HOOK3 Antibody IHC analysis of HOOK3 using anti-HOOK3 antibody (AZA0A8M6Z0P5). HOOK3 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOOK3 Antibody (AZA0A8M6Z0P5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hprt1-antibody-azq7zv49-1-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv49-1-hprt1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HPRT1 Antibody Picoband® Western blot analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPRT1 antigen affinity purified polyclonal antibody (AZQ7ZV49-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HPRT1 at approximately 25 kDa. The expected band size for HPRT1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv49-1-hprt1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HPRT1 Antibody Picoband® IHC analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49-1). HPRT1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HPRT1 Antibody (AZQ7ZV49-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv49-1-hprt1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HPRT1 Antibody Picoband® IHC analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49-1). HPRT1 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HPRT1 Antibody (AZQ7ZV49-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv49-1-hprt1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish HPRT1 Antibody Picoband® IHC analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49-1). HPRT1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HPRT1 Antibody (AZQ7ZV49-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv49-1-hprt1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish HPRT1 Antibody Picoband® IHC analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49-1). HPRT1 was detected in a paraffin-embedded section of zebrafish integument tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HPRT1 Antibody (AZQ7ZV49-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv49-1-hprt1-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish HPRT1 Antibody Picoband® IHC analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49-1). HPRT1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HPRT1 Antibody (AZQ7ZV49-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hrasa-b-antibody-azq568k0-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq568k0-hrasa-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HRASa/b Antibody Picoband® Western blot analysis of HRASa/b using anti-HRASa/b antibody (AZQ568K0). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRASa/b antigen affinity purified polyclonal antibody (AZQ568K0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HRASa/b at approximately 21 kDa. The expected band size for HRASa/b is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq568k0-hrasa-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HRASa/b Antibody Picoband® IHC analysis of HRASa/b using anti-HRASa/b antibody (AZQ568K0). HRASa/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HRASa/b Antibody (AZQ568K0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq568k0-hrasa-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HRASa/b Antibody Picoband® IHC analysis of HRASa/b using anti-HRASa/b antibody (AZQ568K0). HRASa/b was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HRASa/b Antibody (AZQ568K0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq568k0-hrasa-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish HRASa/b Antibody Picoband® IHC analysis of HRASa/b using anti-HRASa/b antibody (AZQ568K0). HRASa/b was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HRASa/b Antibody (AZQ568K0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq568k0-hrasa-b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish HRASa/b Antibody Picoband® IHC analysis of HRASa/b using anti-HRASa/b antibody (AZQ568K0). HRASa/b was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HRASa/b Antibody (AZQ568K0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hs2st1a-b-antibody-aza1l1p8-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l1p8-hs2st1a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HS2ST1a/b Antibody IHC analysis of HS2ST1a/b using anti-HS2ST1a/b antibody (AZA1L1P8). HS2ST1a/b was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HS2ST1a/b Antibody (AZA1L1P8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l1p8-hs2st1a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HS2ST1a/b Antibody IHC analysis of HS2ST1a/b using anti-HS2ST1a/b antibody (AZA1L1P8). HS2ST1a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HS2ST1a/b Antibody (AZA1L1P8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hsp60-hspd1-antibody-azq803b0-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq803b0-hspd1-primary-antibodies-if-testing-1.jpgAnti-Zebrafish HSP60/HSPD1 Antibody Picoband® IF analysis of HSP60/HSPD1 using anti-HSP60/HSPD1 antibody (AZQ803B0). HSP60/HSPD1 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-HSP60/HSPD1 Antibody (AZQ803B0) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq803b0-hspd1-primary-antibodies-wb-testing-2.jpgAnti-Zebrafish HSP60/HSPD1 Antibody Picoband® Western blot analysis of HSP60/HSPD1 using anti-HSP60/HSPD1 antibody (AZQ803B0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP60/HSPD1 antigen affinity purified polyclonal antibody (AZQ803B0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HSP60/HSPD1 at approximately 61 kDa. The expected band size for HSP60/HSPD1 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq803b0-hspd1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HSP60/HSPD1 Antibody Picoband® IHC analysis of HSP60/HSPD1 using anti-HSP60/HSPD1 antibody (AZQ803B0). HSP60/HSPD1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSP60/HSPD1 Antibody (AZQ803B0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq803b0-hspd1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish HSP60/HSPD1 Antibody Picoband® IHC analysis of HSP60/HSPD1 using anti-HSP60/HSPD1 antibody (AZQ803B0). HSP60/HSPD1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSP60/HSPD1 Antibody (AZQ803B0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq803b0-hspd1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish HSP60/HSPD1 Antibody Picoband® IHC analysis of HSP60/HSPD1 using anti-HSP60/HSPD1 antibody (AZQ803B0). HSP60/HSPD1 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSP60/HSPD1 Antibody (AZQ803B0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq803b0-hspd1-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish HSP60/HSPD1 Antibody Picoband® IHC analysis of HSP60/HSPD1 using anti-HSP60/HSPD1 antibody (AZQ803B0). HSP60/HSPD1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSP60/HSPD1 Antibody (AZQ803B0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ide-antibody-aza0a0r4il71-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4il71-ide-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish IDE Antibody Picoband® Western blot analysis of IDE using anti-IDE antibody (AZA0A0R4IL71). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDE antigen affinity purified polyclonal antibody (AZA0A0R4IL71) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IDE at approximately 120 kDa. The expected band size for IDE is at 118 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4il71-ide-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish IDE Antibody Picoband® IHC analysis of IDE using anti-IDE antibody (AZA0A0R4IL71). IDE was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IDE Antibody (AZA0A0R4IL71) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-idh3b-antibody-aza0a2r8qpc9-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8qpc9-idh3b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish IDH3B Antibody IHC analysis of IDH3B using anti-IDH3B antibody (AZA0A2R8QPC9). IDH3B was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IDH3B Antibody (AZA0A2R8QPC9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8qpc9-idh3b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish IDH3B Antibody IHC analysis of IDH3B using anti-IDH3B antibody (AZA0A2R8QPC9). IDH3B was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IDH3B Antibody (AZA0A2R8QPC9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8qpc9-idh3b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish IDH3B Antibody IHC analysis of IDH3B using anti-IDH3B antibody (AZA0A2R8QPC9). IDH3B was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IDH3B Antibody (AZA0A2R8QPC9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8qpc9-idh3b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish IDH3B Antibody IHC analysis of IDH3B using anti-IDH3B antibody (AZA0A2R8QPC9). IDH3B was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IDH3B Antibody (AZA0A2R8QPC9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8qpc9-idh3b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish IDH3B Antibody IHC analysis of IDH3B using anti-IDH3B antibody (AZA0A2R8QPC9). IDH3B was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IDH3B Antibody (AZA0A2R8QPC9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8qpc9-idh3b-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish IDH3B Antibody IHC analysis of IDH3B using anti-IDH3B antibody (AZA0A2R8QPC9). IDH3B was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IDH3B Antibody (AZA0A2R8QPC9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8qpc9-idh3b-primary-antibodies-ihc-testing-7.jpgAnti-Zebrafish IDH3B Antibody IHC analysis of IDH3B using anti-IDH3B antibody (AZA0A2R8QPC9). IDH3B was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IDH3B Antibody (AZA0A2R8QPC9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ift172-antibody-azq5rhh4-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5rhh4-ift172-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish IFT172 Antibody IHC analysis of IFT172 using anti-IFT172 antibody (AZQ5RHH4). IFT172 was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IFT172 Antibody (AZQ5RHH4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nf45-ilf2-antibody-azq6nz06-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nz06-ilf2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NF45/ILF2 Antibody Picoband® Western blot analysis of NF45/ILF2 using anti-NF45/ILF2 antibody (AZQ6NZ06). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF45/ILF2 antigen affinity purified polyclonal antibody (AZQ6NZ06) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NF45/ILF2 at approximately 43 kDa. The expected band size for NF45/ILF2 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nz06-ilf2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish NF45/ILF2 Antibody Picoband® IHC analysis of NF45/ILF2 using anti-NF45/ILF2 antibody (AZQ6NZ06). NF45/ILF2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF45/ILF2 Antibody (AZQ6NZ06) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nz06-ilf2-primary-antibodies-if-testing-1.jpgAnti-Zebrafish NF45/ILF2 Antibody Picoband®IF analysis of NF45/ILF2 using anti-NF45/ILF2 antibody (AZQ6NZ06). NF45/ILF2 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NF45/ILF2 Antibody (AZQ6NZ06) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nz06-ilf2-primary-antibodies-if-testing-2.jpgAnti-Zebrafish NF45/ILF2 Antibody Picoband®IF analysis of NF45/ILF2 using anti-NF45/ILF2 antibody (AZQ6NZ06). NF45/ILF2 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NF45/ILF2 Antibody (AZQ6NZ06) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ilk-antibody-azq6phd6-1-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phd6-1-ilk-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ILK Antibody Picoband® Western blot analysis of ILK using anti-ILK antibody (AZQ6PHD6-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILK antigen affinity purified polyclonal antibody (AZQ6PHD6-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ILK at approximately 51 kDa. The expected band size for ILK is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ilk-antibody-azq6phd6-2-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phd6-2-ilk-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ILK Antibody Picoband® Western blot analysis of ILK using anti-ILK antibody (AZQ6PHD6-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILK antigen affinity purified polyclonal antibody (AZQ6PHD6-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ILK at approximately 51 kDa. The expected band size for ILK is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phd6-2-ilk-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ILK Antibody Picoband® IHC analysis of ILK using anti-ILK antibody (AZQ6PHD6-2). ILK was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ILK Antibody (AZQ6PHD6-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phd6-2-ilk-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish ILK Antibody Picoband® IHC analysis of ILK using anti-ILK antibody (AZQ6PHD6-2). ILK was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ILK Antibody (AZQ6PHD6-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phd6-2-ilk-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish ILK Antibody Picoband® IHC analysis of ILK using anti-ILK antibody (AZQ6PHD6-2). ILK was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ILK Antibody (AZQ6PHD6-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ints12-antibody-azq6iqu7-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqu7-ints12-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish INTS12 Antibody Picoband® Western blot analysis of INTS12 using anti-INTS12 antibody (AZQ6IQU7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INTS12 antigen affinity purified polyclonal antibody (AZQ6IQU7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for INTS12 at approximately 49 kDa. The expected band size for INTS12 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqu7-ints12-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish INTS12 Antibody Picoband® IHC analysis of INTS12 using anti-INTS12 antibody (AZQ6IQU7). INTS12 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-INTS12 Antibody (AZQ6IQU7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqu7-ints12-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish INTS12 Antibody Picoband® IHC analysis of INTS12 using anti-INTS12 antibody (AZQ6IQU7). INTS12 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-INTS12 Antibody (AZQ6IQU7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqu7-ints12-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish INTS12 Antibody Picoband® IHC analysis of INTS12 using anti-INTS12 antibody (AZQ6IQU7). INTS12 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-INTS12 Antibody (AZQ6IQU7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqu7-ints12-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish INTS12 Antibody Picoband® IHC analysis of INTS12 using anti-INTS12 antibody (AZQ6IQU7). INTS12 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-INTS12 Antibody (AZQ6IQU7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqu7-ints12-primary-antibodies-if-testing-1.jpgAnti-Zebrafish INTS12 Antibody Picoband®IF analysis of INTS12 using anti-INTS12 antibody (AZQ6IQU7). INTS12 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-INTS12 Antibody (AZQ6IQU7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-klhl12-antibody-azq5u374-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u374-klhl12-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish KLHL12 Antibody Picoband® Western blot analysis of KLHL12 using anti-KLHL12 antibody (AZQ5U374). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLHL12 antigen affinity purified polyclonal antibody (AZQ5U374) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KLHL12 at approximately 56 kDa. The expected band size for KLHL12 is at 63 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-importin-beta-kpnb1-antibody-aza0a0r4imz8-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4imz8-kpnb1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Importin Beta/KPNB1 Antibody Picoband® Western blot analysis of Importin Beta/KPNB1 using anti-Importin Beta/KPNB1 antibody (AZA0A0R4IMZ8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Importin Beta/KPNB1 antigen affinity purified polyclonal antibody (AZA0A0R4IMZ8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Importin Beta/KPNB1 at approximately 97 kDa. The expected band size for Importin Beta/KPNB1 is at 97 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lims1-antibody-aza0a8m9qhp1-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qhp1-lims1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LIMS1 Antibody Picoband® Western blot analysis of LIMS1 using anti-LIMS1 antibody (AZA0A8M9QHP1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIMS1 antigen affinity purified polyclonal antibody (AZA0A8M9QHP1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LIMS1 at approximately 35 kDa. The expected band size for LIMS1 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qhp1-lims1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish LIMS1 Antibody Picoband® IHC analysis of LIMS1 using anti-LIMS1 antibody (AZA0A8M9QHP1). LIMS1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIMS1 Antibody (AZA0A8M9QHP1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qhp1-lims1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish LIMS1 Antibody Picoband® IHC analysis of LIMS1 using anti-LIMS1 antibody (AZA0A8M9QHP1). LIMS1 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIMS1 Antibody (AZA0A8M9QHP1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lin7c-antibody-azq66ib0-boster.html2026-03-17T05:16:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq66ib0-lin7c-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LIN7C Antibody Picoband® Western blot analysis of LIN7C using anti-LIN7C antibody (AZQ66IB0). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN7C antigen affinity purified polyclonal antibody (AZQ66IB0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LIN7C at approximately 25 kDa. The expected band size for LIN7C is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lin9-antibody-azq5rhq8-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5rhq8-lin9-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish LIN9 Antibody IHC analysis of LIN9 using anti-LIN9 antibody (AZQ5RHQ8). LIN9 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LIN9 Antibody (AZQ5RHQ8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lmo2-antibody-azq9ptj3-1-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9ptj3-1-lmo2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LMO2 Antibody Picoband® Western blot analysis of LMO2 using anti-LMO2 antibody (AZQ9PTJ3-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMO2 antigen affinity purified polyclonal antibody (AZQ9PTJ3-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LMO2 at approximately 22 kDa. The expected band size for LMO2 is at 18 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lonp1-antibody-aza0a0r4ih79-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4ih79-lonp1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish LONP1 Antibody IHC analysis of LONP1 using anti-LONP1 antibody (AZA0A0R4IH79). LONP1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LONP1 Antibody (AZA0A0R4IH79) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lsg1-antibody-azq6ny89-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ny89-lsg1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LSG1 Antibody Picoband® Western blot analysis of LSG1 using anti-LSG1 antibody (AZQ6NY89). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LSG1 antigen affinity purified polyclonal antibody (AZQ6NY89) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LSG1 at approximately 75 kDa. The expected band size for LSG1 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ny89-lsg1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish LSG1 Antibody Picoband® IHC analysis of LSG1 using anti-LSG1 antibody (AZQ6NY89). LSG1 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LSG1 Antibody (AZQ6NY89) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ny89-lsg1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish LSG1 Antibody Picoband® IHC analysis of LSG1 using anti-LSG1 antibody (AZQ6NY89). LSG1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LSG1 Antibody (AZQ6NY89) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-crop-luc7l3-antibody-aza1l1n4-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l1n4-luc7l3-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish CROP/LUC7L3 Antibody IHC analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody (AZA1L1N4). CROP/LUC7L3 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CROP/LUC7L3 Antibody (AZA1L1N4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l1n4-luc7l3-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CROP/LUC7L3 Antibody IHC analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody (AZA1L1N4). CROP/LUC7L3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CROP/LUC7L3 Antibody (AZA1L1N4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l1n4-luc7l3-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish CROP/LUC7L3 Antibody IHC analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody (AZA1L1N4). CROP/LUC7L3 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CROP/LUC7L3 Antibody (AZA1L1N4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l1n4-luc7l3-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish CROP/LUC7L3 Antibody IHC analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody (AZA1L1N4). CROP/LUC7L3 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CROP/LUC7L3 Antibody (AZA1L1N4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l1n4-luc7l3-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish CROP/LUC7L3 Antibody IHC analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody (AZA1L1N4). CROP/LUC7L3 was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CROP/LUC7L3 Antibody (AZA1L1N4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l1n4-luc7l3-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish CROP/LUC7L3 Antibody IHC analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody (AZA1L1N4). CROP/LUC7L3 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CROP/LUC7L3 Antibody (AZA1L1N4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l1n4-luc7l3-primary-antibodies-if-testing-1.jpgAnti-Zebrafish CROP/LUC7L3 AntibodyIF analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody (AZA1L1N4). CROP/LUC7L3 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CROP/LUC7L3 Antibody (AZA1L1N4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lzic-antibody-azq6dhh7-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dhh7-lzic-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish LZIC Antibody IHC analysis of LZIC using anti-LZIC antibody (AZQ6DHH7). LZIC was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LZIC Antibody (AZQ6DHH7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dhh7-lzic-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish LZIC Antibody IHC analysis of LZIC using anti-LZIC antibody (AZQ6DHH7). LZIC was detected in a paraffin-embedded section of spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LZIC Antibody (AZQ6DHH7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-madd-antibody-aza0a8m3aub1-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3aub1-madd-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MADD Antibody Picoband® Western blot analysis of MADD using anti-MADD antibody (AZA0A8M3AUB1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MADD antigen affinity purified polyclonal antibody (AZA0A8M3AUB1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MADD at approximately 200 kDa. The expected band size for MADD is at 183 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-maea-antibody-azq7sxr3-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxr3-maea-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MAEA Antibody Picoband® Western blot analysis of MAEA using anti-MAEA antibody (AZQ7SXR3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAEA antigen affinity purified polyclonal antibody (AZQ7SXR3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAEA at approximately 49 kDa. The expected band size for MAEA is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mat2aa-b-antibody-azf1qyu7-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qyu7-mat2aa-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MAT2Aa/b Antibody Picoband® Western blot analysis of MAT2Aa/b using anti-MAT2Aa/b antibody (AZF1QYU7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT2Aa/b antigen affinity purified polyclonal antibody (AZF1QYU7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAT2Aa/b at approximately 60 kDa. The expected band size for MAT2Aa/b is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qyu7-mat2aa-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish MAT2Aa/b Antibody Picoband® IHC analysis of MAT2Aa/b using anti-MAT2Aa/b antibody (AZF1QYU7). MAT2Aa/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAT2Aa/b Antibody (AZF1QYU7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qyu7-mat2aa-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish MAT2Aa/b Antibody Picoband® IHC analysis of MAT2Aa/b using anti-MAT2Aa/b antibody (AZF1QYU7). MAT2Aa/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAT2Aa/b Antibody (AZF1QYU7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mcm2-antibody-aza0a0r4if65-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4if65-mcm2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MCM2 Antibody Picoband® Western blot analysis of MCM2 using anti-MCM2 antibody (AZA0A0R4IF65). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM2 antigen affinity purified polyclonal antibody (AZA0A0R4IF65) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MCM2 at approximately 125 kDa. The expected band size for MCM2 is at 102 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mcm4-antibody-azq6nzv2-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nzv2-mcm4-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MCM4 Antibody Picoband® Western blot analysis of MCM4 using anti-MCM4 antibody (AZQ6NZV2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM4 antigen affinity purified polyclonal antibody (AZQ6NZV2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MCM4 at approximately 97 kDa. The expected band size for MCM4 is at 97 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-metap2a-b-antibody-aza5wvx8-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza5wvx8-metap2a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish METAP2a/b Antibody Picoband® Western blot analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METAP2a/b antigen affinity purified polyclonal antibody (AZA5WVX8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for METAP2a/b at approximately 67 kDa. The expected band size for METAP2a/b is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza5wvx8-metap2a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish METAP2a/b Antibody Picoband® IHC analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). METAP2a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-METAP2a/b Antibody (AZA5WVX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza5wvx8-metap2a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish METAP2a/b Antibody Picoband® IHC analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). METAP2a/b was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-METAP2a/b Antibody (AZA5WVX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza5wvx8-metap2a-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish METAP2a/b Antibody Picoband® IHC analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). METAP2a/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-METAP2a/b Antibody (AZA5WVX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza5wvx8-metap2a-b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish METAP2a/b Antibody Picoband® IHC analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). METAP2a/b was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-METAP2a/b Antibody (AZA5WVX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza5wvx8-metap2a-b-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish METAP2a/b Antibody Picoband® IHC analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). METAP2a/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-METAP2a/b Antibody (AZA5WVX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza5wvx8-metap2a-b-primary-antibodies-ihc-testing-7.jpgAnti-Zebrafish METAP2a/b Antibody Picoband® IHC analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). METAP2a/b was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-METAP2a/b Antibody (AZA5WVX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza5wvx8-metap2a-b-primary-antibodies-ihc-testing-8.jpgAnti-Zebrafish METAP2a/b Antibody Picoband® IHC analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). METAP2a/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-METAP2a/b Antibody (AZA5WVX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza5wvx8-metap2a-b-primary-antibodies-ihc-testing-9.jpgAnti-Zebrafish METAP2a/b Antibody Picoband® IHC analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). METAP2a/b was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-METAP2a/b Antibody (AZA5WVX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mettl3-antibody-azf1r777-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r777-mettl3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish METTL3 Antibody Picoband® Western blot analysis of METTL3 using anti-METTL3 antibody (AZF1R777). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METTL3 antigen affinity purified polyclonal antibody (AZF1R777) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for METTL3 at approximately 65 kDa. The expected band size for METTL3 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r777-mettl3-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish METTL3 Antibody Picoband® IHC analysis of METTL3 using anti-METTL3 antibody (AZF1R777). METTL3 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-METTL3 Antibody (AZF1R777) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mob3a-b-c-antibody-azq7zw91-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zw91-mob3a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MOB3A/B/C Antibody Picoband® Western blot analysis of MOB3A/B/C using anti-MOB3A/B/C antibody (AZQ7ZW91). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOB3A/B/C antigen affinity purified polyclonal antibody (AZQ7ZW91) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MOB3A/B/C at approximately 23 kDa. The expected band size for MOB3A/B/C is at 25 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-msi1a-b-antibody-azq5czn9-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5czn9-msi1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MSI1a/b Antibody Picoband® Western blot analysis of MSI1a/b using anti-MSI1a/b antibody (AZQ5CZN9). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSI1a/b antigen affinity purified polyclonal antibody (AZQ5CZN9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MSI1a/b at approximately 39 kDa. The expected band size for MSI1a/b is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-myh10-antibody-aza0a8m3ba62-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3ba62-myh10-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MYH10 Antibody Picoband® Western blot analysis of MYH10 using anti-MYH10 antibody (AZA0A8M3BA62). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH10 antigen affinity purified polyclonal antibody (AZA0A8M3BA62) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYH10 at approximately 250 kDa. The expected band size for MYH10 is at 229 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nap1l1-antibody-aza0a8m2bj06-boster.html2026-03-17T05:16:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bj06-nap1l1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NAP1L1 Antibody Picoband® Western blot analysis of NAP1L1 using anti-NAP1L1 antibody (AZA0A8M2BJ06). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAP1L1 antigen affinity purified polyclonal antibody (AZA0A8M2BJ06) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NAP1L1 at approximately 55 kDa. The expected band size for NAP1L1 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ncalda-b-antibody-aza9jth1-boster.html2026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza9jth1-ncalda-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NCALDa/b Antibody Picoband® Western blot analysis of NCALDa/b using anti-NCALDa/b antibody (AZA9JTH1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCALDa/b antigen affinity purified polyclonal antibody (AZA9JTH1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NCALDa/b at approximately 18 kDa. The expected band size for NCALDa/b is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nedd8-8l-antibody-azf1qmf9-boster.html2026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qmf9-nedd8-8l-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish NEDD8/8l Antibody IHC analysis of NEDD8/8l using anti-NEDD8/8l antibody (AZF1QMF9). NEDD8/8l was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEDD8/8l Antibody (AZF1QMF9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qmf9-nedd8-8l-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish NEDD8/8l Antibody IHC analysis of NEDD8/8l using anti-NEDD8/8l antibody (AZF1QMF9). NEDD8/8l was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEDD8/8l Antibody (AZF1QMF9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nol10-antibody-azq802w4-boster.html2026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq802w4-nol10-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish NOL10 Antibody IHC analysis of NOL10 using anti-NOL10 antibody (AZQ802W4). NOL10 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOL10 Antibody (AZQ802W4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq802w4-nol10-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish NOL10 Antibody IHC analysis of NOL10 using anti-NOL10 antibody (AZQ802W4). NOL10 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOL10 Antibody (AZQ802W4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rab3gap1-antibody-azq6nuv0-boster.html2026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nuv0-rab3gap1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RAB3GAP1 Antibody Picoband® Western blot analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (AZQ6NUV0). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB3GAP1 antigen affinity purified polyclonal antibody (AZQ6NUV0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB3GAP1 at approximately 130 kDa. The expected band size for RAB3GAP1 is at 111 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nuv0-rab3gap1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish RAB3GAP1 Antibody Picoband® IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (AZQ6NUV0). RAB3GAP1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (AZQ6NUV0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nuv0-rab3gap1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish RAB3GAP1 Antibody Picoband® IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (AZQ6NUV0). RAB3GAP1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (AZQ6NUV0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nuv0-rab3gap1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish RAB3GAP1 Antibody Picoband® IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (AZQ6NUV0). RAB3GAP1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (AZQ6NUV0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nuv0-rab3gap1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish RAB3GAP1 Antibody Picoband® IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (AZQ6NUV0). RAB3GAP1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (AZQ6NUV0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nuv0-rab3gap1-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish RAB3GAP1 Antibody Picoband® IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (AZQ6NUV0). RAB3GAP1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (AZQ6NUV0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nuv0-rab3gap1-primary-antibodies-ihc-testing-7.jpgAnti-Zebrafish RAB3GAP1 Antibody Picoband® IHC analysis of RAB3GAP1 using anti-RAB3GAP1 antibody (AZQ6NUV0). RAB3GAP1 was detected in a paraffin-embedded section of zebrafish spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB3GAP1 Antibody (AZQ6NUV0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nova2-antibody-azf1r4g7-boster.html2026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r4g7-nova2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NOVA2 Antibody Picoband® Western blot analysis of NOVA2 using anti-NOVA2 antibody (AZF1R4G7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOVA2 antigen affinity purified polyclonal antibody (AZF1R4G7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NOVA2 at approximately 55 kDa. The expected band size for NOVA2 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r4g7-nova2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish NOVA2 Antibody Picoband® IHC analysis of NOVA2 using anti-NOVA2 antibody (AZF1R4G7). NOVA2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOVA2 Antibody (AZF1R4G7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r4g7-nova2-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish NOVA2 Antibody Picoband® IHC analysis of NOVA2 using anti-NOVA2 antibody (AZF1R4G7). NOVA2 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOVA2 Antibody (AZF1R4G7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r4g7-nova2-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish NOVA2 Antibody Picoband® IHC analysis of NOVA2 using anti-NOVA2 antibody (AZF1R4G7). NOVA2 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOVA2 Antibody (AZF1R4G7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pfn2a-b-antibody-azq6aza1-boster.html2026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6aza1-pfn2a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PFN2a/b Antibody Picoband® Western blot analysis of PFN2a/b using anti-PFN2a/b antibody (AZQ6AZA1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFN2a/b antigen affinity purified polyclonal antibody (AZQ6AZA1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PFN2a/b at approximately 15 kDa. The expected band size for PFN2a/b is at 15 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psmc4-antibody-azb0r1d0-boster.html2026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0r1d0-psmc4-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PSMC4 Antibody Picoband® Western blot analysis of PSMC4 using anti-PSMC4 antibody (AZB0R1D0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC4 antigen affinity purified polyclonal antibody (AZB0R1D0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMC4 at approximately 47 kDa. The expected band size for PSMC4 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0r1d0-psmc4-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PSMC4 Antibody Picoband® IHC analysis of PSMC4 using anti-PSMC4 antibody (AZB0R1D0). PSMC4 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC4 Antibody (AZB0R1D0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-opa1-antibody-azq5u3a7-boster.html2026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u3a7-opa1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish OPA1 Antibody Picoband® Western blot analysis of OPA1 using anti-OPA1 antibody (AZQ5U3A7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA1 antigen affinity purified polyclonal antibody (AZQ5U3A7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OPA1 at approximately 80 kDa. The expected band size for OPA1 is at 112 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u3a7-opa1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish OPA1 Antibody Picoband® IHC analysis of OPA1 using anti-OPA1 antibody (AZQ5U3A7). OPA1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OPA1 Antibody (AZQ5U3A7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u3a7-opa1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish OPA1 Antibody Picoband® IHC analysis of OPA1 using anti-OPA1 antibody (AZQ5U3A7). OPA1 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OPA1 Antibody (AZQ5U3A7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dj-1-park7-antibody-azq5xj36-boster.html2026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5xj36-park7-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DJ-1/PARK7 Antibody Picoband® Western blot analysis of DJ-1/PARK7 using anti-DJ-1/PARK7 antibody (AZQ5XJ36). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DJ-1/PARK7 antigen affinity purified polyclonal antibody (AZQ5XJ36) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DJ-1/PARK7 at approximately 20 kDa. The expected band size for DJ-1/PARK7 is at 20 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pax2a-b-antibody-azq90268-boster.html2026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90268-pax2a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PAX2a/b Antibody Picoband® Western blot analysis of PAX2a/b using anti-PAX2a/b antibody (AZQ90268). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX2a/b antigen affinity purified polyclonal antibody (AZQ90268) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAX2a/b at approximately 40 kDa. The expected band size for PAX2a/b is at 45 kDa. https://www.bosterbio.com/catalog/product/view/id/1298642026-03-10T04:40:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1298652026-03-10T04:40:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1298682026-03-10T04:40:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0471.pngRat NGF/NGF Beta ELISA Kit EZ-Set™ (DIY Antibody Pairs)Rat NGF/NGF Beta EZ Set ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-eif4eb-antibody.html2026-03-17T05:16:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1298702026-03-10T04:40:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1298712026-03-10T04:40:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1298722026-03-10T04:40:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1298732026-03-10T04:40:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1298742026-03-10T04:40:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1298752026-03-10T04:40:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1298762026-03-10T04:40:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1298772026-03-10T04:40:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1298782026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02571-2-mbd3-primary-antibodies-wb-testing-1.jpgAnti-MBD3 Antibody Picoband®Western blot analysis of MBD3 using anti-MBD3 antibody (A02571-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBD3 antigen affinity purified polyclonal antibody (A02571-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MBD3 at approximately 33 kDa. The expected band size for MBD3 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02571-2-mbd3-primary-antibodies-if-testing-1.jpgAnti-MBD3 Antibody Picoband®IF analysis of MBD3 using anti-MBD3 antibody (A02571-2) and anti-Beta Tubulin antibody (M01857-3). MBD3 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MBD3 Antibody (A02571-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1298792026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07019-3-mc5r-primary-antibodies-wb-testing-1.jpgAnti-MC5R Antibody Picoband®Western blot analysis of MC5R using anti-MC5R antibody (A07019-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC5R antigen affinity purified polyclonal antibody (A07019-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MC5R at approximately 37 kDa. The expected band size for MC5R is at 37 kDa. https://www.bosterbio.com/catalog/product/view/id/1298802026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05167-2-meox2-primary-antibodies-wb-testing-1.jpgAnti-MEOX2 Antibody Picoband®Western blot analysis of MEOX2 using anti-MEOX2 antibody (A05167-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEOX2 antigen affinity purified polyclonal antibody (A05167-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MEOX2 at approximately 34 kDa. The expected band size for MEOX2 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05167-2-meox2-primary-antibodies-fcm-testing-1.jpgAnti-MEOX2 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-MEOX2 antibody (A05167-2). Overlay histogram showing A549 cells stained with A05167-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEOX2 Antibody (A05167-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298812026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01647-3-gne-primary-antibodies-wb-testing-1.jpgAnti-GNE Antibody Picoband®Western blot analysis of GNE using anti-GNE antibody (A01647-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNE antigen affinity purified polyclonal antibody (A01647-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GNE at approximately 79 kDa. The expected band size for GNE is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01647-3-gne-primary-antibodies-ihc-testing-1.jpgAnti-GNE Antibody Picoband®IHC analysis of GNE using anti-GNE antibody (A01647-3). GNE was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNE Antibody (A01647-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01647-3-gne-primary-antibodies-ihc-testing-2.jpgAnti-GNE Antibody Picoband®IHC analysis of GNE using anti-GNE antibody (A01647-3). GNE was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNE Antibody (A01647-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01647-3-gne-primary-antibodies-fcm-testing-1.jpgAnti-GNE Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-GNE antibody (A01647-3). Overlay histogram showing HeLa cells stained with A01647-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNE Antibody (A01647-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298822026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13029-1-gtf2a2-primary-antibodies-wb-testing-1.jpgAnti-GTF2A2 Antibody Picoband®Western blot analysis of GTF2A2 using anti-GTF2A2 antibody (A13029-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GTF2A2 antigen affinity purified polyclonal antibody (A13029-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GTF2A2 at approximately 17 kDa. The expected band size for GTF2A2 is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13029-1-gtf2a2-primary-antibodies-fcm-testing-1.jpgAnti-GTF2A2 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-GTF2A2 antibody (A13029-1). Overlay histogram showing U251 cells stained with A13029-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GTF2A2 Antibody (A13029-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298832026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00928-3-gss-primary-antibodies-wb-testing-1.jpgAnti-GSS Antibody Picoband®Western blot analysis of GSS using anti-GSS antibody (A00928-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSS antigen affinity purified polyclonal antibody (A00928-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSS at approximately 50 kDa. The expected band size for GSS is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00928-3-gss-primary-antibodies-ihc-testing-1.jpgAnti-GSS Antibody Picoband®IHC analysis of GSS using anti-GSS antibody (A00928-3). GSS was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSS Antibody (A00928-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00928-3-gss-primary-antibodies-ihc-testing-2.jpgAnti-GSS Antibody Picoband®IHC analysis of GSS using anti-GSS antibody (A00928-3). GSS was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSS Antibody (A00928-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00928-3-gss-primary-antibodies-fcm-testing-1.jpgAnti-GSS Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-GSS antibody (A00928-3). Overlay histogram showing 293T cells stained with A00928-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSS Antibody (A00928-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298842026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00928-4-gss-primary-antibodies-wb-testing-1.jpgAnti-GSS Antibody Picoband®Western blot analysis of GSS using anti-GSS antibody (A00928-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSS antigen affinity purified polyclonal antibody (A00928-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSS at approximately 52 kDa. The expected band size for GSS is at 52 kDa. https://www.bosterbio.com/catalog/product/view/id/1298852026-03-17T05:16:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06893-gtf2b-primary-antibodies-wb-testing-1.jpgAnti-GTF2B Antibody Picoband®Western blot analysis of GTF2B using anti-GTF2B antibody (A06893). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GTF2B antigen affinity purified polyclonal antibody (A06893) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GTF2B at approximately 35 kDa. The expected band size for GTF2B is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06893-gtf2b-primary-antibodies-ihc-testing-1.jpgAnti-GTF2B Antibody Picoband®IHC analysis of GTF2B using anti-GTF2B antibody (A06893). GTF2B was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GTF2B Antibody (A06893) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06893-gtf2b-primary-antibodies-fcm-testing-1.jpgAnti-GTF2B Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-GTF2B antibody (A06893). Overlay histogram showing HepG2 cells stained with A06893 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GTF2B Antibody (A06893, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298862026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05856-1-h6pd-primary-antibodies-wb-testing-1.jpgAnti-H6PD Antibody Picoband®Western blot analysis of H6PD using anti-H6PD antibody (A05856-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H6PD antigen affinity purified polyclonal antibody (A05856-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for H6PD at approximately 89 kDa. The expected band size for H6PD is at 89 kDa. https://www.bosterbio.com/catalog/product/view/id/1298872026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02612-1-hal-primary-antibodies-fcm-testing-1.jpgAnti-HAL Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-HAL antibody (A02612-1). Overlay histogram showing Jurkat cells stained with A02612-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HAL Antibody (A02612-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02612-1-hal-primary-antibodies-ihc-testing-1.jpgAnti-HAL Antibody Picoband®IHC analysis of HAL using anti-HAL antibody (A02612-1). HAL was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HAL Antibody (A02612-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02612-1-hal-primary-antibodies-ihc-testing-2.jpgAnti-HAL Antibody Picoband®IHC analysis of HAL using anti-HAL antibody (A02612-1). HAL was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HAL Antibody (A02612-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02612-1-hal-primary-antibodies-if-testing-1.jpgAnti-HAL Antibody Picoband®IF analysis of HAL using anti-HAL antibody (A02612-1). HAL was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-HAL Antibody (A02612-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02612-1-hal-primary-antibodies-if-testing-2.jpgAnti-HAL Antibody Picoband®IF analysis of HAL using anti-HAL antibody (A02612-1). HAL was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-HAL Antibody (A02612-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02612-1-hal-primary-antibodies-wb-testing-1.jpgAnti-HAL Antibody Picoband®Western blot analysis of HAL using anti-HAL antibody (A02612-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAL antigen affinity purified polyclonal antibody (A02612-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HAL at approximately 75 kDa. The expected band size for HAL is at 73 kDa. https://www.bosterbio.com/catalog/product/view/id/1298882026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05316-1-heph-primary-antibodies-wb-testing-1.jpgAnti-Hephaestin/HEPH Antibody Picoband®Western blot analysis of Hephaestin/HEPH using anti-Hephaestin/HEPH antibody (A05316-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hephaestin/HEPH antigen affinity purified polyclonal antibody (A05316-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Hephaestin/HEPH at approximately 130 kDa. The expected band size for Hephaestin/HEPH is at 130 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05316-1-heph-primary-antibodies-ihc-testing-1.jpgAnti-Hephaestin/HEPH Antibody Picoband®IHC analysis of Hephaestin/HEPH using anti-Hephaestin/HEPH antibody (A05316-1). Hephaestin/HEPH was detected in a paraffin-embedded section of human small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hephaestin/HEPH Antibody (A05316-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05316-1-heph-primary-antibodies-fcm-testing-1.jpgAnti-Hephaestin/HEPH Antibody Picoband®Flow Cytometry analysis of EL-4 cells using anti-Hephaestin/HEPH antibody (A05316-1). Overlay histogram showing EL-4 cells stained with A05316-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Hephaestin/HEPH Antibody (A05316-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298892026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05145-3-hk3-primary-antibodies-wb-testing-1.jpgAnti-HK3 Antibody Picoband®Western blot analysis of HK3 using anti-HK3 antibody (A05145-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HK3 antigen affinity purified polyclonal antibody (A05145-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HK3 at approximately 99 kDa. The expected band size for HK3 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05145-3-hk3-primary-antibodies-ihc-testing-2.jpgAnti-HK3 Antibody Picoband®IHC analysis of HK3 using anti-HK3 antibody (A05145-3). HK3 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HK3 Antibody (A05145-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05145-3-hk3-primary-antibodies-ihc-testing-1.jpgAnti-HK3 Antibody Picoband®IHC analysis of HK3 using anti-HK3 antibody (A05145-3). HK3 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HK3 Antibody (A05145-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05145-3-hk3-primary-antibodies-fcm-testing-1.jpgAnti-HK3 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-HK3 antibody (A05145-3). Overlay histogram showing THP-1 cells stained with A05145-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HK3 Antibody (A05145-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298902026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02116-2-hif1an-primary-antibodies-wb-testing-1.jpgAnti-HIF1AN Antibody Picoband®Western blot analysis of HIF1AN using anti-HIF1AN antibody (A02116-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1AN antigen affinity purified polyclonal antibody (A02116-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HIF1AN at approximately 40 kDa. The expected band size for HIF1AN is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02116-2-hif1an-primary-antibodies-fcm-testing-1.jpgAnti-HIF1AN Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-HIF1AN antibody (A02116-2). Overlay histogram showing Jurkat cells stained with A02116-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIF1AN Antibody (A02116-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298912026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02377-1-hlcs-primary-antibodies-wb-testing-1.jpgAnti-HLCS Antibody Picoband®Western blot analysis of HLCS using anti-HLCS antibody (A02377-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLCS antigen affinity purified polyclonal antibody (A02377-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HLCS at approximately 81 kDa. The expected band size for HLCS is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02377-1-hlcs-primary-antibodies-ihc-testing-1.jpgAnti-HLCS Antibody Picoband®IHC analysis of HLCS using anti-HLCS antibody (A02377-1). HLCS was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HLCS Antibody (A02377-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02377-1-hlcs-primary-antibodies-fcm-testing-1.jpgAnti-HLCS Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-HLCS antibody (A02377-1). Overlay histogram showing RT4 cells stained with A02377-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HLCS Antibody (A02377-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298922026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01336-3-aaas-primary-antibodies-wb-testing-1.jpgAnti-ALADIN/AAAS Antibody Picoband®Western blot analysis of ALADIN/AAAS using anti-ALADIN/AAAS antibody (A01336-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALADIN/AAAS antigen affinity purified polyclonal antibody (A01336-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALADIN/AAAS at approximately 60 kDa. The expected band size for ALADIN/AAAS is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01336-3-aaas-primary-antibodies-fcm-testing-1.jpgAnti-ALADIN/AAAS Antibody Picoband®Flow Cytometry analysis of U2OS cells using anti-ALADIN/AAAS antibody (A01336-3). Overlay histogram showing U2OS cells stained with A01336-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALADIN/AAAS Antibody (A01336-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298932026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07199-1-cmklr2-primary-antibodies-wb-testing-1.jpgAnti-CMKLR2 Antibody Picoband®Western blot analysis of CMKLR2 using anti-CMKLR2 antibody (A07199-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CMKLR2 antigen affinity purified polyclonal antibody (A07199-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CMKLR2 at approximately 41 kDa. The expected band size for CMKLR2 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07199-1-cmklr2-primary-antibodies-fcm-testing-1.jpgAnti-CMKLR2 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-CMKLR2 antibody (A07199-1). Overlay histogram showing U251 cells stained with A07199-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CMKLR2 Antibody (A07199-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298942026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02971-1-pygm-primary-antibodies-wb-testing-1.jpgAnti-PYGM Antibody Picoband®Western blot analysis of PYGM using anti-PYGM antibody (A02971-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYGM antigen affinity purified polyclonal antibody (A02971-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PYGM at approximately 97 kDa. The expected band size for PYGM is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02971-1-pygm-primary-antibodies-ihc-testing-1.jpgAnti-PYGM Antibody Picoband®IHC analysis of PYGM using anti-PYGM antibody (A02971-1). PYGM was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYGM Antibody (A02971-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02971-1-pygm-primary-antibodies-ihc-testing-2.jpgAnti-PYGM Antibody Picoband®IHC analysis of PYGM using anti-PYGM antibody (A02971-1). PYGM was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYGM Antibody (A02971-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02971-1-pygm-primary-antibodies-ihc-testing-3.jpgAnti-PYGM Antibody Picoband®IHC analysis of PYGM using anti-PYGM antibody (A02971-1). PYGM was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYGM Antibody (A02971-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02971-1-pygm-primary-antibodies-ihc-testing-4.jpgAnti-PYGM Antibody Picoband®IHC analysis of PYGM using anti-PYGM antibody (A02971-1). PYGM was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYGM Antibody (A02971-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02971-1-pygm-primary-antibodies-ihc-testing-5.jpgAnti-PYGM Antibody Picoband®IHC analysis of PYGM using anti-PYGM antibody (A02971-1). PYGM was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYGM Antibody (A02971-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02971-1-pygm-primary-antibodies-ihc-testing-6.jpgAnti-PYGM Antibody Picoband®IHC analysis of PYGM using anti-PYGM antibody (A02971-1). PYGM was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYGM Antibody (A02971-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02971-1-pygm-primary-antibodies-ihc-testing-7.jpgAnti-PYGM Antibody Picoband®IHC analysis of PYGM using anti-PYGM antibody (A02971-1). PYGM was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYGM Antibody (A02971-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02971-1-pygm-primary-antibodies-fcm-testing-1.jpgAnti-PYGM Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-PYGM antibody (A02971-1). Overlay histogram showing Jurkat cells stained with A02971-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYGM Antibody (A02971-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298952026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06112-2-gstk1-primary-antibodies-wb-testing-1.jpgAnti-GSTK1 Antibody Picoband®Western blot analysis of GSTK1 using anti-GSTK1 antibody (A06112-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTK1 antigen affinity purified polyclonal antibody (A06112-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSTK1 at approximately 26 kDa. The expected band size for GSTK1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06112-2-gstk1-primary-antibodies-fcm-testing-1.jpgAnti-GSTK1 Antibody Picoband®Flow Cytometry analysis of HEPA1-6 cells using anti-GSTK1 antibody (A06112-2). Overlay histogram showing HEPA1-6 cells stained with A06112-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSTK1 Antibody (A06112-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298962026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10720-1-pwp1-primary-antibodies-wb-testing-1.jpgAnti-PWP1 Antibody Picoband®Western blot analysis of PWP1 using anti-PWP1 antibody (A10720-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PWP1 antigen affinity purified polyclonal antibody (A10720-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PWP1 at approximately 50 kDa. The expected band size for PWP1 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10720-1-pwp1-primary-antibodies-fcm-testing-1.jpgAnti-PWP1 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-PWP1 antibody (A10720-1). Overlay histogram showing Jurkat cells stained with A10720-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PWP1 Antibody (A10720-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298972026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10517-1-ndufa10-primary-antibodies-wb-testing-1.jpgAnti-NDUFA10 Antibody Picoband®Western blot analysis of NDUFA10 using anti-NDUFA10 antibody (A10517-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA10 antigen affinity purified polyclonal antibody (A10517-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NDUFA10 at approximately 41 kDa. The expected band size for NDUFA10 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10517-1-ndufa10-primary-antibodies-ihc-testing-2.jpgAnti-NDUFA10 Antibody Picoband®IHC analysis of NDUFA10 using anti-NDUFA10 antibody (A10517-1). NDUFA10 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFA10 Antibody (A10517-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10517-1-ndufa10-primary-antibodies-ihc-testing-1.jpgAnti-NDUFA10 Antibody Picoband®IHC analysis of NDUFA10 using anti-NDUFA10 antibody (A10517-1). NDUFA10 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFA10 Antibody (A10517-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10517-1-ndufa10-primary-antibodies-ihc-testing-3.jpgAnti-NDUFA10 Antibody Picoband®IHC analysis of NDUFA10 using anti-NDUFA10 antibody (A10517-1). NDUFA10 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFA10 Antibody (A10517-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10517-1-ndufa10-primary-antibodies-if-testing-1.jpgAnti-NDUFA10 Antibody Picoband®IF analysis of NDUFA10 using anti-NDUFA10 antibody (A10517-1). NDUFA10 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NDUFA10 Antibody (A10517-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10517-1-ndufa10-primary-antibodies-if-testing-2.jpgAnti-NDUFA10 Antibody Picoband®IF analysis of NDUFA10 using anti-NDUFA10 antibody (A10517-1). NDUFA10 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NDUFA10 Antibody (A10517-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10517-1-ndufa10-primary-antibodies-if-testing-3.jpgAnti-NDUFA10 Antibody Picoband®IF analysis of NDUFA10 using anti-NDUFA10 antibody (A10517-1). NDUFA10 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NDUFA10 Antibody (A10517-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10517-1-ndufa10-primary-antibodies-fcm-testing-1.jpgAnti-NDUFA10 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-NDUFA10 antibody (A10517-1). Overlay histogram showing RT4 cells stained with A10517-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA10 Antibody (A10517-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1298982026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06481-1-neu2-primary-antibodies-wb-testing-1.jpgAnti-NEU2 Antibody Picoband®Western blot analysis of NEU2 using anti-NEU2 antibody (A06481-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEU2 antigen affinity purified polyclonal antibody (A06481-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NEU2 at approximately 42 kDa. The expected band size for NEU2 is at 42 kDa. https://www.bosterbio.com/catalog/product/view/id/1298992026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01038-2-neurod1-primary-antibodies-wb-testing-1.jpgAnti-NEUROD1 Antibody Picoband®Western blot analysis of NEUROD1 using anti-NEUROD1 antibody (A01038-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEUROD1 antigen affinity purified polyclonal antibody (A01038-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NEUROD1 at approximately 50 kDa. The expected band size for NEUROD1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01038-2-neurod1-primary-antibodies-fcm-testing-1.jpgAnti-NEUROD1 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-NEUROD1 antibody (A01038-2). Overlay histogram showing U251 cells stained with A01038-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEUROD1 Antibody (A01038-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299002026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00483-2-mpl-primary-antibodies-wb-testing-1.jpgAnti-TPOR/MPL Antibody Picoband®Western blot analysis of TPOR/MPL using anti-TPOR/MPL antibody (A00483-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPOR/MPL antigen affinity purified polyclonal antibody (A00483-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TPOR/MPL at approximately 90 kDa. The expected band size for TPOR/MPL is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00483-2-mpl-primary-antibodies-fcm-testing-1.jpgAnti-TPOR/MPL Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-TPOR/MPL antibody (A00483-2). Overlay histogram showing HEL cells stained with A00483-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TPOR/MPL Antibody (A00483-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299012026-03-17T05:16:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06048-magi3-primary-antibodies-wb-testing-1.jpgAnti-MAGI3 Antibody Picoband®Western blot analysis of MAGI3 using anti-MAGI3 antibody (A06048). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87-MG whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAGI3 antigen affinity purified polyclonal antibody (A06048) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAGI3 at approximately 130 kDa. The expected band size for MAGI3 is at 163 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06048-magi3-primary-antibodies-fcm-testing-1.jpgAnti-MAGI3 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-MAGI3 antibody (A06048). Overlay histogram showing 293T cells stained with A06048 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MAGI3 Antibody (A06048, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299022026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05933-2-neurog1-primary-antibodies-wb-testing-1.jpgAnti-NEUROG1 Antibody Picoband®Western blot analysis of NEUROG1 using anti-NEUROG1 antibody (A05933-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEUROG1 antigen affinity purified polyclonal antibody (A05933-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NEUROG1 at approximately 26 kDa. The expected band size for NEUROG1 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05933-2-neurog1-primary-antibodies-ihc-testing-1.jpgAnti-NEUROG1 Antibody Picoband®IHC analysis of NEUROG1 using anti-NEUROG1 antibody (A05933-2). NEUROG1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEUROG1 Antibody (A05933-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1299032026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08533-2-map1lc3c-primary-antibodies-wb-testing-1.jpgAnti-MAP1LC3C Antibody Picoband®Western blot analysis of MAP1LC3C using anti-MAP1LC3C antibody (A08533-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP1LC3C antigen affinity purified polyclonal antibody (A08533-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAP1LC3C at approximately 17 kDa. The expected band size for MAP1LC3C is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08533-2-map1lc3c-primary-antibodies-ihc-testing-1.jpgAnti-MAP1LC3C Antibody Picoband®IHC analysis of MAP1LC3C using anti-MAP1LC3C antibody (A08533-2). MAP1LC3C was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAP1LC3C Antibody (A08533-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08533-2-map1lc3c-primary-antibodies-ihc-testing-2.jpgAnti-MAP1LC3C Antibody Picoband®IHC analysis of MAP1LC3C using anti-MAP1LC3C antibody (A08533-2). MAP1LC3C was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAP1LC3C Antibody (A08533-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08533-2-map1lc3c-primary-antibodies-ihc-testing-3.jpgAnti-MAP1LC3C Antibody Picoband®IHC analysis of MAP1LC3C using anti-MAP1LC3C antibody (A08533-2). MAP1LC3C was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAP1LC3C Antibody (A08533-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08533-2-map1lc3c-primary-antibodies-ihc-testing-4.jpgAnti-MAP1LC3C Antibody Picoband®IHC analysis of MAP1LC3C using anti-MAP1LC3C antibody (A08533-2). MAP1LC3C was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAP1LC3C Antibody (A08533-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1299042026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04806-1-mcat-primary-antibodies-wb-testing-1.jpgAnti-MCAT Antibody Picoband®Western blot analysis of MCAT using anti-MCAT antibody (A04806-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCAT antigen affinity purified polyclonal antibody (A04806-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MCAT at approximately 39 kDa. The expected band size for MCAT is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04806-1-mcat-primary-antibodies-ihc-testing-1.jpgAnti-MCAT Antibody Picoband®IHC analysis of MCAT using anti-MCAT antibody (A04806-1). MCAT was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCAT Antibody (A04806-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04806-1-mcat-primary-antibodies-ihc-testing-2.jpgAnti-MCAT Antibody Picoband®IHC analysis of MCAT using anti-MCAT antibody (A04806-1). MCAT was detected in a paraffin-embedded section of mouse eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCAT Antibody (A04806-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04806-1-mcat-primary-antibodies-ihc-testing-3.jpgAnti-MCAT Antibody Picoband®IHC analysis of MCAT using anti-MCAT antibody (A04806-1). MCAT was detected in a paraffin-embedded section of rat eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCAT Antibody (A04806-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04806-1-mcat-primary-antibodies-if-testing-1.jpgAnti-MCAT Antibody Picoband®IF analysis of MCAT using anti-MCAT antibody (A04806-1) and anti-Beta Tubulin antibody (M01857-3). MCAT was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MCAT Antibody (A04806-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04806-1-mcat-primary-antibodies-ip-testing-1.jpgAnti-MCAT Antibody Picoband®Immunoprecipitating MCAT in Hela whole cell lysate. Western blot analysis of MCAT using anti-MCAT antibody (A04806-1). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-MCAT antibody in Hela whole cell lysate, Lane 3: anti-MCAT antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MCAT antigen affinity purified polyclonal antibody (A04806-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MCAT at approximately 39 kDa. The expected band size for MCAT is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04806-1-mcat-primary-antibodies-fcm-testing-1.jpgAnti-MCAT Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-MCAT antibody (A04806-1). Overlay histogram showing RT4 cells stained with A04806-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCAT Antibody (A04806-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04806-1-mcat-primary-antibodies-ihc-testing-5.jpgAnti-MCAT Antibody Picoband®IHC analysis of MCAT using anti-MCAT antibody (A04806-1). MCAT was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCAT Antibody (A04806-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1299052026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02666-2-mcph1-primary-antibodies-wb-testing-1.jpgAnti-BRIT1/MCPH1 Antibody Picoband®Western blot analysis of BRIT1/MCPH1 using anti-BRIT1/MCPH1 antibody (A02666-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRIT1/MCPH1 antigen affinity purified polyclonal antibody (A02666-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BRIT1/MCPH1 at approximately 93 kDa. The expected band size for BRIT1/MCPH1 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02666-2-mcph1-primary-antibodies-if-testing-1.jpgAnti-BRIT1/MCPH1 Antibody Picoband®IF analysis of BRIT1/MCPH1 using anti-BRIT1/MCPH1 antibody (A02666-2) and anti-Beta Tubulin antibody (M01857-3). BRIT1/MCPH1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BRIT1/MCPH1 Antibody (A02666-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02666-2-mcph1-primary-antibodies-fcm-testing-1.jpgAnti-BRIT1/MCPH1 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-BRIT1/MCPH1 antibody (A02666-2). Overlay histogram showing 293T cells stained with A02666-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRIT1/MCPH1 Antibody (A02666-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299062026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02968-2-aptx-primary-antibodies-wb-testing-1.jpgAnti-APTX Antibody Picoband®Western blot analysis of APTX using anti-APTX antibody (A02968-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APTX antigen affinity purified polyclonal antibody (A02968-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for APTX at approximately 41 kDa. The expected band size for APTX is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02968-2-aptx-primary-antibodies-fcm-testing-1.jpgAnti-APTX Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-APTX antibody (A02968-2). Overlay histogram showing HepG2 cells stained with A02968-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APTX Antibody (A02968-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299072026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08842-1-mtbp-primary-antibodies-wb-testing-1.jpgAnti-MTBP Antibody Picoband®Western blot analysis of MTBP using anti-MTBP antibody (A08842-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTBP antigen affinity purified polyclonal antibody (A08842-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MTBP at approximately 100 kDa. The expected band size for MTBP is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08842-1-mtbp-primary-antibodies-fcm-testing-1.jpgAnti-MTBP Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-MTBP antibody (A08842-1). Overlay histogram showing PC-3 cells stained with A08842-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTBP Antibody (A08842-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299082026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07098-1-mib2-primary-antibodies-wb-testing-1.jpgAnti-MIB2 Antibody Picoband®Western blot analysis of MIB2 using anti-MIB2 antibody (A07098-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIB2 antigen affinity purified polyclonal antibody (A07098-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MIB2 at approximately 110 kDa. The expected band size for MIB2 is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07098-1-mib2-primary-antibodies-fcm-testing-1.jpgAnti-MIB2 Antibody Picoband®Flow Cytometry analysis of SiHa cells using anti-MIB2 antibody (A07098-1). Overlay histogram showing SiHa cells stained with A07098-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIB2 Antibody (A07098-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07098-1-mib2-primary-antibodies-ip-testing-1.jpgAnti-MIB2 Antibody Picoband®Immunoprecipitating (IP) MIB2 in A431 whole cell lysate. Western blot analysis of MIB2 using anti-MIB2 antibody (A07098-1); Lane 1: A431 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-MIB2 antibody in A431 whole cell lysate; Lane 3: anti-MIB2 antibody (2μg) + A431 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MIB2 antigen affinity purified polyclonal antibody (A07098-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for MIB2 at approximately 110 kDa. The expected band size for MIB2 is at 110 kDa. https://www.bosterbio.com/catalog/product/view/id/1299092026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06692-2-ereg-primary-antibodies-wb-testing-1.jpgAnti-EREG Antibody Picoband®Western blot analysis of EREG using anti-EREG antibody (A06692-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EREG antigen affinity purified polyclonal antibody (A06692-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EREG at approximately 19 kDa. The expected band size for EREG is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06692-2-ereg-primary-antibodies-ihc-testing-1.jpgAnti-EREG Antibody Picoband®IHC analysis of EREG using anti-EREG antibody (A06692-2). EREG was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EREG Antibody (A06692-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06692-2-ereg-primary-antibodies-ihc-testing-2.jpgAnti-EREG Antibody Picoband®IHC analysis of EREG using anti-EREG antibody (A06692-2). EREG was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EREG Antibody (A06692-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06692-2-ereg-primary-antibodies-fcm-testing-1.jpgAnti-EREG Antibody Picoband®Flow Cytometry analysis of CACO-2 cells using anti-EREG antibody (A06692-2). Overlay histogram showing CACO-2 cells stained with A06692-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EREG Antibody (A06692-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299102026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05539-1-eif4h-primary-antibodies-wb-testing-1.jpgAnti-EIF4H Antibody Picoband®Western blot analysis of EIF4H using anti-EIF4H antibody (A05539-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4H antigen affinity purified polyclonal antibody (A05539-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EIF4H at approximately 25 kDa. The expected band size for EIF4H is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05539-1-eif4h-primary-antibodies-if-testing-1.jpgAnti-EIF4H Antibody Picoband®IF analysis of EIF4H using anti-EIF4H antibody (A05539-1). EIF4H was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EIF4H Antibody (A05539-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05539-1-eif4h-primary-antibodies-fcm-testing-1.jpgAnti-EIF4H Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-EIF4H antibody (A05539-1). Overlay histogram showing Jurkat cells stained with A05539-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF4H Antibody (A05539-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299112026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08322-2-camlg-primary-antibodies-wb-testing-1.jpgAnti-CAMLG Antibody Picoband®Western blot analysis of CAMLG using anti-CAMLG antibody (A08322-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: rat ovary tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAMLG antigen affinity purified polyclonal antibody (A08322-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CAMLG at approximately 36 kDa. The expected band size for CAMLG is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08322-2-camlg-primary-antibodies-ihc-testing-1.jpgAnti-CAMLG Antibody Picoband®IHC analysis of CAMLG using anti-CAMLG antibody (A08322-2). CAMLG was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAMLG Antibody (A08322-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08322-2-camlg-primary-antibodies-ihc-testing-2.jpgAnti-CAMLG Antibody Picoband®IHC analysis of CAMLG using anti-CAMLG antibody (A08322-2). CAMLG was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAMLG Antibody (A08322-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08322-2-camlg-primary-antibodies-ihc-testing-3.jpgAnti-CAMLG Antibody Picoband®IHC analysis of CAMLG using anti-CAMLG antibody (A08322-2). CAMLG was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAMLG Antibody (A08322-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08322-2-camlg-primary-antibodies-ihc-testing-4.jpgAnti-CAMLG Antibody Picoband®IHC analysis of CAMLG using anti-CAMLG antibody (A08322-2). CAMLG was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAMLG Antibody (A08322-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08322-2-camlg-primary-antibodies-fcm-testing-1.jpgAnti-CAMLG Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-CAMLG antibody (A08322-2). Overlay histogram showing 293T cells stained with A08322-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CAMLG Antibody (A08322-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299122026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06609-1-calm1-2-3-primary-antibodies-wb-testing-1.jpgAnti-Calmodulin/CALM 1/2/3 Antibody Picoband®Western blot analysis of Calmodulin/CALM 1/2/3 using anti-Calmodulin/CALM 1/2/3 antibody (A06609-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. .After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calmodulin/CALM 1/2/3 antigen affinity purified polyclonal antibody (A06609-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Calmodulin/CALM 1/2/3 at approximately 17 kDa. The expected band size for Calmodulin/CALM 1/2/3 is at 17 kDa. https://www.bosterbio.com/catalog/product/view/id/1299132026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08904-dock3-primary-antibodies-wb-testing-1.jpgAnti-MOCA/DOCK3 Antibody Picoband®Western blot analysis of MOCA/DOCK3 using anti-MOCA/DOCK3 antibody (A08904). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOCA/DOCK3 antigen affinity purified polyclonal antibody (A08904) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MOCA/DOCK3 at approximately 233 kDa. The expected band size for MOCA/DOCK3 is at 233 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08904-dock3-primary-antibodies-ihc-testing-1.jpgAnti-MOCA/DOCK3 Antibody Picoband®IHC analysis of MOCA/DOCK3 using anti-MOCA/DOCK3 antibody (A08904). MOCA/DOCK3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MOCA/DOCK3 Antibody (A08904) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08904-dock3-primary-antibodies-ihc-testing-2.jpgAnti-MOCA/DOCK3 Antibody Picoband®IHC analysis of MOCA/DOCK3 using anti-MOCA/DOCK3 antibody (A08904). MOCA/DOCK3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MOCA/DOCK3 Antibody (A08904) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08904-dock3-primary-antibodies-fcm-testing-1.jpgAnti-MOCA/DOCK3 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-MOCA/DOCK3 antibody (A08904). Overlay histogram showing 293T cells stained with A08904 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOCA/DOCK3 Antibody (A08904, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299142026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02350-1-hpn-primary-antibodies-wb-testing-1.jpgAnti-Hepsin/HPN Antibody Picoband®Western blot analysis of Hepsin/HPN using anti-Hepsin/HPN antibody (A02350-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hepsin/HPN antigen affinity purified polyclonal antibody (A02350-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Hepsin/HPN at approximately 51 kDa. The expected band size for Hepsin/HPN is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02350-1-hpn-primary-antibodies-ihc-testing-1.jpgAnti-Hepsin/HPN Antibody Picoband®IHC analysis of Hepsin/HPN using anti-Hepsin/HPN antibody (A02350-1). Hepsin/HPN was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hepsin/HPN Antibody (A02350-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02350-1-hpn-primary-antibodies-ihc-testing-2.jpgAnti-Hepsin/HPN Antibody Picoband®IHC analysis of Hepsin/HPN using anti-Hepsin/HPN antibody (A02350-1). Hepsin/HPN was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hepsin/HPN Antibody (A02350-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02350-1-hpn-primary-antibodies-ihc-testing-3.jpgAnti-Hepsin/HPN Antibody Picoband®IHC analysis of Hepsin/HPN using anti-Hepsin/HPN antibody (A02350-1). Hepsin/HPN was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hepsin/HPN Antibody (A02350-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02350-1-hpn-primary-antibodies-fcm-testing-1.jpgAnti-Hepsin/HPN Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-Hepsin/HPN antibody (A02350-1). Overlay histogram showing HepG2 cells stained with A02350-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Hepsin/HPN Antibody (A02350-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299152026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09319-2-map4k2-primary-antibodies-wb-testing-1.jpgAnti-MAP4K2 Antibody Picoband®Western blot analysis of MAP4K2 using anti-MAP4K2 antibody (A09319-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse 3T3-L1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP4K2 antigen affinity purified polyclonal antibody (A09319-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAP4K2 at approximately 97 kDa. The expected band size for MAP4K2 is at 92 kDa. https://www.bosterbio.com/catalog/product/view/id/1299162026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03608-3-ndufs4-primary-antibodies-wb-testing-1.jpgAnti-NDUFS4 Antibody Picoband®Western blot analysis of NDUFS4 using anti-NDUFS4 antibody (A03608-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS4 antigen affinity purified polyclonal antibody (A03608-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NDUFS4 at approximately 20 kDa. The expected band size for NDUFS4 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03608-3-ndufs4-primary-antibodies-ihc-testing-1.jpgAnti-NDUFS4 Antibody Picoband®IHC analysis of NDUFS4 using anti-NDUFS4 antibody (A03608-3). NDUFS4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS4 Antibody (A03608-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03608-3-ndufs4-primary-antibodies-ihc-testing-2.jpgAnti-NDUFS4 Antibody Picoband®IHC analysis of NDUFS4 using anti-NDUFS4 antibody (A03608-3). NDUFS4 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS4 Antibody (A03608-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03608-3-ndufs4-primary-antibodies-ihc-testing-3.jpgAnti-NDUFS4 Antibody Picoband®IHC analysis of NDUFS4 using anti-NDUFS4 antibody (A03608-3). NDUFS4 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS4 Antibody (A03608-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03608-3-ndufs4-primary-antibodies-ihc-testing-4.jpgAnti-NDUFS4 Antibody Picoband®IHC analysis of NDUFS4 using anti-NDUFS4 antibody (A03608-3). NDUFS4 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFS4 Antibody (A03608-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03608-3-ndufs4-primary-antibodies-fcm-testing-1.jpgAnti-NDUFS4 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-NDUFS4 antibody (A03608-3). Overlay histogram showing 293T cells stained with A03608-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS4 Antibody (A03608-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299172026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13227-1-fbxo21-primary-antibodies-wb-testing-1.jpgAnti-FBXO21 Antibody Picoband®Western blot analysis of FBXO21 using anti-FBXO21 antibody (A13227-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse ovary tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXO21 antigen affinity purified polyclonal antibody (A13227-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXO21 at approximately 72 kDa. The expected band size for FBXO21 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13227-1-fbxo21-primary-antibodies-ihc-testing-1.jpgAnti-FBXO21 Antibody Picoband®IHC analysis of FBXO21 using anti-FBXO21 antibody (A13227-1). FBXO21 was detected in a paraffin-embedded section of human fallopian tube tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FBXO21 Antibody (A13227-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13227-1-fbxo21-primary-antibodies-fcm-testing-1.jpgAnti-FBXO21 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-FBXO21 antibody (A13227-1). Overlay histogram showing HepG2 cells stained with A13227-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FBXO21 Antibody (A13227-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299182026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06497-3-gtse1-primary-antibodies-wb-testing-1.jpgAnti-GTSE1 Antibody Picoband®Western blot analysis of GTSE1 using anti-GTSE1 antibody (A06497-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GTSE1 antigen affinity purified polyclonal antibody (A06497-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GTSE1 at approximately 100 kDa. The expected band size for GTSE1 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06497-3-gtse1-primary-antibodies-fcm-testing-1.jpgAnti-GTSE1 Antibody Picoband®Flow Cytometry analysis of ANA-1 cells using anti-GTSE1 antibody (A06497-3). Overlay histogram showing ANA-1 cells stained with A06497-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GTSE1 Antibody (A06497-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299192026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02544-2-pwwp3a-primary-antibodies-wb-testing-1.jpgAnti-MUM1/PWWP3A Antibody Picoband®Western blot analysis of MUM1/PWWP3A using anti-MUM1/PWWP3A antibody (A02544-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUM1/PWWP3A antigen affinity purified polyclonal antibody (A02544-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MUM1/PWWP3A at approximately 79 kDa. The expected band size for MUM1/PWWP3A is at 79 kDa. https://www.bosterbio.com/catalog/product/view/id/1299202026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04580-1-myof-primary-antibodies-wb-testing-1.jpgAnti-MYOF Antibody Picoband®Western blot analysis of MYOF using anti-MYOF antibody (A04580-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat L6 whole cell lysates, Lane 4: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYOF antigen affinity purified polyclonal antibody (A04580-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYOF at approximately 250 kDa. The expected band size for MYOF is at 235 kDa. https://www.bosterbio.com/catalog/product/view/id/1299212026-03-17T05:16:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04163-1-naga-primary-antibodies-wb-testing-1.jpgAnti-NAGA Antibody Picoband®Western blot analysis of NAGA using anti-NAGA antibody (A04163-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse small intestine tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAGA antigen affinity purified polyclonal antibody (A04163-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NAGA at approximately 60 kDa. The expected band size for NAGA is at 47 kDa. https://www.bosterbio.com/catalog/product/view/id/1299222026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02709-4-nkx3-1-primary-antibodies-wb-testing-1.jpgAnti-NKX3-1 Antibody Picoband®Western blot analysis of NKX3-1 using anti-NKX3-1 antibody (A02709-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKX3-1 antigen affinity purified polyclonal antibody (A02709-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NKX3-1 at approximately 36 kDa. The expected band size for NKX3-1 is at 26 kDa. https://www.bosterbio.com/catalog/product/view/id/1299232026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02709-5-nkx3-1-primary-antibodies-wb-testing-1.jpgAnti-NKX3-1 Antibody Picoband®Western blot analysis of NKX3-1 using anti-NKX3-1 antibody (A02709-5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat HBZY-1 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse kidney tissue lysates. Lane 7: mouse brain tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKX3-1 antigen affinity purified polyclonal antibody (A02709-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NKX3-1 at approximately 36 kDa. The expected band size for NKX3-1 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02709-5-nkx3-1-primary-antibodies-fcm-testing-1.jpgAnti-NKX3-1 Antibody Picoband®Flow Cytometry analysis of RAW264.7 cells using anti-NKX3-1 antibody (A02709-5). Overlay histogram showing RAW264.7 cells stained with A02709-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NKX3-1 Antibody (A02709-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299242026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07228-1-fhod1-primary-antibodies-wb-testing-1.jpgAnti-FHOD1 Antibody Picoband®Western blot analysis of FHOD1 using anti-FHOD1 antibody (A07228-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHOD1 antigen affinity purified polyclonal antibody (A07228-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FHOD1 at approximately 140 kDa. The expected band size for FHOD1 is at 127 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07228-1-fhod1-primary-antibodies-fcm-testing-1.jpgAnti-FHOD1 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-FHOD1 antibody (A07228-1). Overlay histogram showing HEL cells stained with A07228-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FHOD1 Antibody (A07228-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299252026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04948-1-nlgn1-primary-antibodies-wb-testing-1.jpgAnti-NLGN1 Antibody Picoband®Western blot analysis of NLGN1 using anti-NLGN1 antibody (A04948-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLGN1 antigen affinity purified polyclonal antibody (A04948-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NLGN1 at approximately 116 kDa. The expected band size for NLGN1 is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04948-1-nlgn1-primary-antibodies-if-testing-1.jpgAnti-NLGN1 Antibody Picoband®IF analysis of NLGN1 using anti-NLGN1 antibody (A04948-1). NLGN1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NLGN1 Antibody (A04948-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1299262026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01729-2-hcfc1-primary-antibodies-wb-testing-1.jpgAnti-HCFC1 Antibody Picoband®Western blot analysis of HCFC1 using anti-HCFC1 antibody (A01729-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse C2C12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCFC1 antigen affinity purified polyclonal antibody (A01729-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HCFC1 at approximately 130 kDa. The expected band size for HCFC1 is at 209 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01729-2-hcfc1-primary-antibodies-ihc-testing-1.jpgAnti-HCFC1 Antibody Picoband®IHC analysis of HCFC1 using anti-HCFC1 antibody (A01729-2). HCFC1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HCFC1 Antibody (A01729-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01729-2-hcfc1-primary-antibodies-ihc-testing-2.jpgAnti-HCFC1 Antibody Picoband®IHC analysis of HCFC1 using anti-HCFC1 antibody (A01729-2). HCFC1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HCFC1 Antibody (A01729-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01729-2-hcfc1-primary-antibodies-ihc-testing-3.jpgAnti-HCFC1 Antibody Picoband®IHC analysis of HCFC1 using anti-HCFC1 antibody (A01729-2). HCFC1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HCFC1 Antibody (A01729-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01729-2-hcfc1-primary-antibodies-ihc-testing-4.jpgAnti-HCFC1 Antibody Picoband®IHC analysis of HCFC1 using anti-HCFC1 antibody (A01729-2). HCFC1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HCFC1 Antibody (A01729-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01729-2-hcfc1-primary-antibodies-if-testing-1.jpgAnti-HCFC1 Antibody Picoband®IF analysis of HCFC1 using anti-HCFC1 antibody (A01729-2) and anti-Beta Tubulin antibody (M01857-3). HCFC1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HCFC1 Antibody (A01729-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01729-2-hcfc1-primary-antibodies-ip-testing-1.jpgAnti-HCFC1 Antibody Picoband®Immunoprecipitating HCFC1 in K562 whole cell lysate. Western blot analysis of HCFC1 using anti-HCFC1 antibody (A01729-2). Lane 1: K562 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-HCFC1 antibody in K562 whole cell lysate, Lane 3: anti-HCFC1 antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HCFC1 antigen affinity purified polyclonal antibody (A01729-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HCFC1 at approximately 130 kDa. The expected band size for HCFC1 is at 209 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01729-2-hcfc1-primary-antibodies-fcm-testing-1.jpgAnti-HCFC1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-HCFC1 antibody (A01729-2). Overlay histogram showing HepG2 cells stained with A01729-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HCFC1 Antibody (A01729-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299272026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05760-1-nkx6-1-primary-antibodies-wb-testing-1.jpgAnti-NKX6-1 Antibody Picoband®Western blot analysis of NKX6-1 using anti-NKX6-1 antibody (A05760-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKX6-1 antigen affinity purified polyclonal antibody (A05760-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NKX6-1 at approximately 50 kDa. The expected band size for NKX6-1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05760-1-nkx6-1-primary-antibodies-ihc-testing-1.jpgAnti-NKX6-1 Antibody Picoband®IHC analysis of NKX6-1 using anti-NKX6-1 antibody (A05760-1). NKX6-1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NKX6-1 Antibody (A05760-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05760-1-nkx6-1-primary-antibodies-if-testing-1.jpgAnti-NKX6-1 Antibody Picoband®IF analysis of NKX6-1 using anti-NKX6-1 antibody (A05760-1) and anti-Beta Tubulin antibody (M01857-3). NKX6-1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NKX6-1 Antibody (A05760-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05760-1-nkx6-1-primary-antibodies-fcm-testing-1.jpgAnti-NKX6-1 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-NKX6-1 antibody (A05760-1). Overlay histogram showing 293T cells stained with A05760-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NKX6-1 Antibody (A05760-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299282026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01004-4-mok-primary-antibodies-wb-testing-1.jpgAnti-MOK Antibody Picoband®Western blot analysis of MOK using anti-MOK antibody (A01004-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOK antigen affinity purified polyclonal antibody (A01004-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MOK at approximately 48 kDa. The expected band size for MOK is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01004-4-mok-primary-antibodies-fcm-testing-1.jpgAnti-MOK Antibody Picoband®Flow Cytometry analysis of A431 cells using anti-MOK antibody (A01004-4). Overlay histogram showing A431 cells stained with A01004-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOK Antibody (A01004-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299292026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06465-2-mthfd2-primary-antibodies-wb-testing-1.jpgAnti-MTHFD2 Antibody Picoband®Western blot analysis of MTHFD2 using anti-MTHFD2 antibody (A06465-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTHFD2 antigen affinity purified polyclonal antibody (A06465-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MTHFD2 at approximately 35 kDa. The expected band size for MTHFD2 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06465-2-mthfd2-primary-antibodies-fcm-testing-1.jpgAnti-MTHFD2 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-MTHFD2 antibody (A06465-2). Overlay histogram showing A549 cells stained with A06465-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTHFD2 Antibody (A06465-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299302026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13986-nkg7-primary-antibodies-wb-testing-1.jpgAnti-NKG7 Antibody Picoband®Western blot analysis of NKG7 using anti-NKG7 antibody (A13986). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKG7 antigen affinity purified polyclonal antibody (A13986) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NKG7 at approximately 18-25 kDa. The expected band size for NKG7 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13986-nkg7-primary-antibodies-ihc-testing-1.jpgAnti-NKG7 Antibody Picoband®IHC analysis of NKG7 using anti-NKG7 antibody (A13986). NKG7 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NKG7 Antibody (A13986) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1299312026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00749-1-mtm1-primary-antibodies-wb-testing-1.jpgAnti-MTM1 Antibody Picoband®Western blot analysis of MTM1 using anti-MTM1 antibody (A00749-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTM1 antigen affinity purified polyclonal antibody (A00749-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MTM1 at approximately 65 kDa. The expected band size for MTM1 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00749-1-mtm1-primary-antibodies-ihc-testing-1.jpgAnti-MTM1 Antibody Picoband®IHC analysis of MTM1 using anti-MTM1 antibody (A00749-1). MTM1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTM1 Antibody (A00749-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00749-1-mtm1-primary-antibodies-ihc-testing-2.jpgAnti-MTM1 Antibody Picoband®IHC analysis of MTM1 using anti-MTM1 antibody (A00749-1). MTM1 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTM1 Antibody (A00749-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00749-1-mtm1-primary-antibodies-ihc-testing-3.jpgAnti-MTM1 Antibody Picoband®IHC analysis of MTM1 using anti-MTM1 antibody (A00749-1). MTM1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTM1 Antibody (A00749-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00749-1-mtm1-primary-antibodies-if-testing-1.jpgAnti-MTM1 Antibody Picoband®IF analysis of MTM1 using anti-MTM1 antibody (A00749-1). MTM1 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MTM1 Antibody (A00749-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1299322026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01401-1-mtrr-primary-antibodies-wb-testing-1.jpgAnti-MTRR Antibody Picoband®Western blot analysis of MTRR using anti-MTRR antibody (A01401-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTRR antigen affinity purified polyclonal antibody (A01401-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MTRR at approximately 78 kDa. The expected band size for MTRR is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01401-1-mtrr-primary-antibodies-ihc-testing-1.jpgAnti-MTRR Antibody Picoband®IHC analysis of MTRR using anti-MTRR antibody (A01401-1). MTRR was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTRR Antibody (A01401-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01401-1-mtrr-primary-antibodies-if-testing-1.jpgAnti-MTRR Antibody Picoband®IF analysis of MTRR using anti-MTRR antibody (A01401-1). MTRR was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MTRR Antibody (A01401-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01401-1-mtrr-primary-antibodies-ip-testing-1.jpgAnti-MTRR Antibody Picoband®Immunoprecipitating (IP) MTRR in Hela whole cell lysate. Western blot analysis of MTRR using anti-MTRR antibody (A01401-1); Lane 1: Hela whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-MTRR antibody in Hela whole cell lysate; Lane 3: anti-MTRR antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MTRR antigen affinity purified polyclonal antibody (A01401-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for MTRR at approximately 78 kDa. The expected band size for MTRR is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01401-1-mtrr-primary-antibodies-fcm-testing-1.jpgAnti-MTRR Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-MTRR antibody (A01401-1). Overlay histogram showing A549 cells stained with A01401-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTRR Antibody (A01401-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01401-1-mtrr-primary-antibodies-fcm-testing-2.jpgAnti-MTRR Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-MTRR antibody (A01401-1). Overlay histogram showing K562 cells stained with A01401-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MTRR Antibody (A01401-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299332026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06583-2-ncam2-primary-antibodies-wb-testing-1.jpgAnti-NCAM2 Antibody Picoband®Western blot analysis of NCAM2 using anti-NCAM2 antibody (A06583-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAM2 antigen affinity purified polyclonal antibody (A06583-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NCAM2 at approximately 140 kDa. The expected band size for NCAM2 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06583-2-ncam2-primary-antibodies-if-testing-1.jpgAnti-NCAM2 Antibody Picoband®IF analysis of NCAM2 using anti-NCAM2 antibody (A06583-2) and anti-Beta Tubulin antibody (M01857-3). NCAM2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NCAM2 Antibody (A06583-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06583-2-ncam2-primary-antibodies-ip-testing-1.jpgAnti-NCAM2 Antibody Picoband®Immunoprecipitating NCAM2 in MCF-7 whole cell lysate. Western blot analysis of NCAM2 using anti-NCAM2 antibody (A06583-2). Lane 1: MCF-7 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-NCAM2 antibody in MCF-7 whole cell lysate, Lane 3: anti-NCAM2 antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NCAM2 antigen affinity purified polyclonal antibody (A06583-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NCAM2 at approximately 140 kDa. The expected band size for NCAM2 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06583-2-ncam2-primary-antibodies-fcm-testing-1.jpgAnti-NCAM2 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-NCAM2 antibody (A06583-2). Overlay histogram showing THP-1 cells stained with A06583-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NCAM2 Antibody (A06583-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1299342026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04740-2-nkx2-2-primary-antibodies-ihc-testing-1.jpgAnti-NKX2-2 AntibodyIHC analysis of NKX2-2 using anti-NKX2-2 antibody (A04740-2). NKX2-2 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NKX2-2 Antibody (A04740-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04740-2-nkx2-2-primary-antibodies-ihc-testing-2.jpgAnti-NKX2-2 AntibodyIHC analysis of NKX2-2 using anti-NKX2-2 antibody (A04740-2). NKX2-2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NKX2-2 Antibody (A04740-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04740-2-nkx2-2-primary-antibodies-ihc-testing-3.jpgAnti-NKX2-2 AntibodyIHC analysis of NKX2-2 using anti-NKX2-2 antibody (A04740-2). NKX2-2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NKX2-2 Antibody (A04740-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04740-2-nkx2-2-primary-antibodies-ihc-testing-4.jpgAnti-NKX2-2 AntibodyIHC analysis of NKX2-2 using anti-NKX2-2 antibody (A04740-2). NKX2-2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NKX2-2 Antibody (A04740-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04740-2-nkx2-2-primary-antibodies-if-testing-1.jpgAnti-NKX2-2 AntibodyIF analysis of NKX2-2 using anti-NKX2-2 antibody (A04740-2) and anti-Beta Tubulin antibody (M01857-3). NKX2-2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NKX2-2 Antibody (A04740-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1299352026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09210-1-nkx2-3-primary-antibodies-ihc-testing-1.jpgAnti-NKX2-3 AntibodyIHC analysis of NKX2-3 using anti-NKX2-3 antibody (A09210-1). NKX2-3 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NKX2-3 Antibody (A09210-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-npepps-antibody-aza0a8m1nse3-boster.html2026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1nse3-npepps-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish NPEPPS AntibodyIHC analysis of NPEPPS using anti-NPEPPS antibody (AZA0A8M1NSE3). NPEPPS was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPEPPS Antibody (AZA0A8M1NSE3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1nse3-npepps-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish NPEPPS AntibodyIHC analysis of NPEPPS using anti-NPEPPS antibody (AZA0A8M1NSE3). NPEPPS was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPEPPS Antibody (AZA0A8M1NSE3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1nse3-npepps-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish NPEPPS AntibodyIHC analysis of NPEPPS using anti-NPEPPS antibody (AZA0A8M1NSE3). NPEPPS was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPEPPS Antibody (AZA0A8M1NSE3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nploc4-antibody-azq7zvi8-boster.html2026-03-17T05:16:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvi8-nploc4-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish NPLOC4 AntibodyIHC analysis of NPLOC4 using anti-NPLOC4 antibody (AZQ7ZVI8). NPLOC4 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPLOC4 Antibody (AZQ7ZVI8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvi8-nploc4-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish NPLOC4 AntibodyIHC analysis of NPLOC4 using anti-NPLOC4 antibody (AZQ7ZVI8). NPLOC4 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPLOC4 Antibody (AZQ7ZVI8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-neuropeptide-y-npy-antibody-azq9i8p3-boster.html2026-03-17T05:16:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i8p3-npy-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Neuropeptide Y/NPY AntibodyIHC analysis of NPY using anti-NPY antibody (AZQ9I8P3). NPY was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPY Antibody (AZQ9I8P3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i8p3-npy-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Neuropeptide Y/NPY AntibodyIHC analysis of NPY using anti-NPY antibody (AZQ9I8P3). NPY was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPY Antibody (AZQ9I8P3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i8p3-npy-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish Neuropeptide Y/NPY AntibodyIHC analysis of NPY using anti-NPY antibody (AZQ9I8P3). NPY was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPY Antibody (AZQ9I8P3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i8p3-npy-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish Neuropeptide Y/NPY AntibodyIHC analysis of NPY using anti-NPY antibody (AZQ9I8P3). NPY was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPY Antibody (AZQ9I8P3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i8p3-npy-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish Neuropeptide Y/NPY AntibodyIHC analysis of NPY using anti-NPY antibody (AZQ9I8P3). NPY was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPY Antibody (AZQ9I8P3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i8p3-npy-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish Neuropeptide Y/NPY AntibodyIHC analysis of NPY using anti-NPY antibody (AZQ9I8P3). NPY was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPY Antibody (AZQ9I8P3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-oat-antibody-azf8w5l3-boster.html2026-03-17T05:16:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf8w5l3-oat-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish OAT AntibodyIHC analysis of OAT using anti-OAT antibody (AZF8W5L3). OAT was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT Antibody (AZF8W5L3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf8w5l3-oat-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish OAT AntibodyIHC analysis of OAT using anti-OAT antibody (AZF8W5L3). OAT was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT Antibody (AZF8W5L3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf8w5l3-oat-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish OAT AntibodyIHC analysis of OAT using anti-OAT antibody (AZF8W5L3). OAT was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT Antibody (AZF8W5L3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf8w5l3-oat-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish OAT AntibodyIHC analysis of OAT using anti-OAT antibody (AZF8W5L3). OAT was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT Antibody (AZF8W5L3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf8w5l3-oat-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish OAT AntibodyIHC analysis of OAT using anti-OAT antibody (AZF8W5L3). OAT was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT Antibody (AZF8W5L3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf8w5l3-oat-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish OAT AntibodyIHC analysis of OAT using anti-OAT antibody (AZF8W5L3). OAT was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT Antibody (AZF8W5L3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ostf1-antibody-azq6tgw5-boster.html2026-03-17T05:16:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tgw5-ostf1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish OSTF1 Antibody Picoband®Western blot analysis of OSTF1 using anti-OSTF1 antibody (AZQ6TGW5). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSTF1 antigen affinity purified polyclonal antibody (AZQ6TGW5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OSTF1 at approximately 28 kDa. The expected band size for OSTF1 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tgw5-ostf1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish OSTF1 Antibody Picoband®IHC analysis of OSTF1 using anti-OSTF1 antibody (AZQ6TGW5). OSTF1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OSTF1 Antibody (AZQ6TGW5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tgw5-ostf1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish OSTF1 Antibody Picoband®IHC analysis of OSTF1 using anti-OSTF1 antibody (AZQ6TGW5). OSTF1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OSTF1 Antibody (AZQ6TGW5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tgw5-ostf1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish OSTF1 Antibody Picoband®IHC analysis of OSTF1 using anti-OSTF1 antibody (AZQ6TGW5). OSTF1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OSTF1 Antibody (AZQ6TGW5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tgw5-ostf1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish OSTF1 Antibody Picoband®IHC analysis of OSTF1 using anti-OSTF1 antibody (AZQ6TGW5). OSTF1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OSTF1 Antibody (AZQ6TGW5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tgw5-ostf1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish OSTF1 Antibody Picoband®IHC analysis of OSTF1 using anti-OSTF1 antibody (AZQ6TGW5). OSTF1 was detected in a paraffin-embedded section of zebrafish fins tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OSTF1 Antibody (AZQ6TGW5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tgw5-ostf1-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish OSTF1 Antibody Picoband®IHC analysis of OSTF1 using anti-OSTF1 antibody (AZQ6TGW5). OSTF1 was detected in a paraffin-embedded section of zebrafish spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OSTF1 Antibody (AZQ6TGW5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-otud5a-b-antibody-azq08bw0-boster.html2026-03-17T05:16:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq08bw0-otud5a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish OTUD5a/b Antibody Picoband®Western blot analysis of OTUD5a/b using anti-OTUD5a/b antibody (AZQ08BW0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUD5a/b antigen affinity purified polyclonal antibody (AZQ08BW0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OTUD5a/b at approximately 75 kDa. The expected band size for OTUD5a/b is at 61 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pa2g4a-b-antibody-azq8aw82-boster.html2026-03-17T05:16:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8aw82-pa2g4a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PA2G4a/b AntibodyIHC analysis of PA2G4a/b using anti-PA2G4a/b antibody (AZQ8AW82). PA2G4a/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PA2G4a/b Antibody (AZQ8AW82) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8aw82-pa2g4a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PA2G4a/b AntibodyIHC analysis of PA2G4a/b using anti-PA2G4a/b antibody (AZQ8AW82). PA2G4a/b was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PA2G4a/b Antibody (AZQ8AW82) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8aw82-pa2g4a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PA2G4a/b AntibodyIHC analysis of PA2G4a/b using anti-PA2G4a/b antibody (AZQ8AW82). PA2G4a/b was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PA2G4a/b Antibody (AZQ8AW82) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8aw82-pa2g4a-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish PA2G4a/b AntibodyIHC analysis of PA2G4a/b using anti-PA2G4a/b antibody (AZQ8AW82). PA2G4a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PA2G4a/b Antibody (AZQ8AW82) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pabpc4-antibody-aza0a8m9pe33-boster.html2026-03-17T05:16:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9pe33-pabpc4-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PABPC4 AntibodyIHC analysis of PABPC4 using anti-PABPC4 antibody (AZA0A8M9PE33). PABPC4 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PABPC4 Antibody (AZA0A8M9PE33) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9pe33-pabpc4-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PABPC4 AntibodyIHC analysis of PABPC4 using anti-PABPC4 antibody (AZA0A8M9PE33). PABPC4 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PABPC4 Antibody (AZA0A8M9PE33) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9pe33-pabpc4-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PABPC4 AntibodyIHC analysis of PABPC4 using anti-PABPC4 antibody (AZA0A8M9PE33). PABPC4 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PABPC4 Antibody (AZA0A8M9PE33) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pafah1b1a-b-antibody-azq7t394-boster.html2026-03-17T05:16:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t394-pafah1b1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PAFAH1B1a/b Antibody Picoband®Western blot analysis of PAFAH1B1a/b using anti-PAFAH1B1a/b antibody (AZQ7T394). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAFAH1B1a/b antigen affinity purified polyclonal antibody (AZQ7T394) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAFAH1B1a/b at approximately 47 kDa. The expected band size for PAFAH1B1a/b is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t394-pafah1b1a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PAFAH1B1a/b Antibody Picoband®IHC analysis of PAFAH1B1a/b using anti-PAFAH1B1a/b antibody (AZQ7T394). PAFAH1B1a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAFAH1B1a/b Antibody (AZQ7T394) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t394-pafah1b1a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PAFAH1B1a/b Antibody Picoband®IHC analysis of PAFAH1B1a/b using anti-PAFAH1B1a/b antibody (AZQ7T394). PAFAH1B1a/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAFAH1B1a/b Antibody (AZQ7T394) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t394-pafah1b1a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PAFAH1B1a/b Antibody Picoband®IHC analysis of PAFAH1B1a/b using anti-PAFAH1B1a/b antibody (AZQ7T394). PAFAH1B1a/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAFAH1B1a/b Antibody (AZQ7T394) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t394-pafah1b1a-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish PAFAH1B1a/b Antibody Picoband®IHC analysis of PAFAH1B1a/b using anti-PAFAH1B1a/b antibody (AZQ7T394). PAFAH1B1a/b was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAFAH1B1a/b Antibody (AZQ7T394) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t394-pafah1b1a-b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish PAFAH1B1a/b Antibody Picoband®IHC analysis of PAFAH1B1a/b using anti-PAFAH1B1a/b antibody (AZQ7T394). PAFAH1B1a/b was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAFAH1B1a/b Antibody (AZQ7T394) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pard3aa-antibody-aza0a8m6yy70-boster.html2026-03-17T05:16:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yy70-pard3aa-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PARD3aa AntibodyIHC analysis of PARD3aa using anti-PARD3aa antibody (AZA0A8M6YY70). PARD3aa was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARD3aa Antibody (AZA0A8M6YY70) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yy70-pard3aa-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PARD3aa AntibodyIHC analysis of PARD3aa using anti-PARD3aa antibody (AZA0A8M6YY70). PARD3aa was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARD3aa Antibody (AZA0A8M6YY70) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yy70-pard3aa-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PARD3aa AntibodyIHC analysis of PARD3aa using anti-PARD3aa antibody (AZA0A8M6YY70). PARD3aa was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARD3aa Antibody (AZA0A8M6YY70) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yy70-pard3aa-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish PARD3aa AntibodyIHC analysis of PARD3aa using anti-PARD3aa antibody (AZA0A8M6YY70). PARD3aa was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARD3aa Antibody (AZA0A8M6YY70) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yy70-pard3aa-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish PARD3aa AntibodyIHC analysis of PARD3aa using anti-PARD3aa antibody (AZA0A8M6YY70). PARD3aa was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARD3aa Antibody (AZA0A8M6YY70) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yy70-pard3aa-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish PARD3aa AntibodyIHC analysis of PARD3aa using anti-PARD3aa antibody (AZA0A8M6YY70). PARD3aa was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARD3aa Antibody (AZA0A8M6YY70) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yy70-pard3aa-primary-antibodies-ihc-testing-7.jpgAnti-Zebrafish PARD3aa AntibodyIHC analysis of PARD3aa using anti-PARD3aa antibody (AZA0A8M6YY70). PARD3aa was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARD3aa Antibody (AZA0A8M6YY70) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pccb-antibody-azb0v0x1-boster.html2026-03-17T05:16:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0v0x1-pccb-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PCCB Antibody Picoband®Western blot analysis of PCCB using anti-PCCB antibody (AZB0V0X1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCCB antigen affinity purified polyclonal antibody (AZB0V0X1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCCB at approximately 58 kDa. The expected band size for PCCB is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pdcd10a-b-antibody-azq6phh3-boster.html2026-03-17T05:16:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phh3-pdcd10a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PDCD10a/b AntibodyIHC analysis of PDCD10a/b using anti-PDCD10a/b antibody (AZQ6PHH3). PDCD10a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDCD10a/b Antibody (AZQ6PHH3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phh3-pdcd10a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PDCD10a/b AntibodyIHC analysis of PDCD10a/b using anti-PDCD10a/b antibody (AZQ6PHH3). PDCD10a/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDCD10a/b Antibody (AZQ6PHH3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phh3-pdcd10a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PDCD10a/b AntibodyIHC analysis of PDCD10a/b using anti-PDCD10a/b antibody (AZQ6PHH3). PDCD10a/b was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDCD10a/b Antibody (AZQ6PHH3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pgam1a-b-antibody-azq7szr4-boster.html2026-03-17T05:16:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7szr4-pgam1a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PGAM1a/b AntibodyIHC analysis of PGAM1a/b using anti-PGAM1a/b antibody (AZQ7SZR4). PGAM1a/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGAM1a/b Antibody (AZQ7SZR4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-phyhipl-b-antibody-aza4qnw7-boster.html2026-03-17T05:16:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza4qnw7-phyhipl-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PHYHIPL/b AntibodyIHC analysis of PHYHIPL/b using anti-PHYHIPL/b antibody (AZA4QNW7). PHYHIPL/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PHYHIPL/b Antibody (AZA4QNW7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza4qnw7-phyhipl-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PHYHIPL/b AntibodyIHC analysis of PHYHIPL/b using anti-PHYHIPL/b antibody (AZA4QNW7). PHYHIPL/b was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PHYHIPL/b Antibody (AZA4QNW7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pi4kb-antibody-azq49gp3-boster.html2026-03-17T05:16:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq49gp3-pi4kb-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PI4KB AntibodyIHC analysis of PI4KB using anti-PI4KB antibody (AZQ49GP3). PI4KB was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PI4KB Antibody (AZQ49GP3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq49gp3-pi4kb-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PI4KB AntibodyIHC analysis of PI4KB using anti-PI4KB antibody (AZQ49GP3). PI4KB was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PI4KB Antibody (AZQ49GP3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-polr2a-antibody-aza0a0r4ims9-boster.html2026-03-17T05:16:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4ims9-polr2a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish POLR2A Antibody Picoband®Western blot analysis of POLR2A using anti-POLR2A antibody (AZA0A0R4IMS9). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2A antigen affinity purified polyclonal antibody (AZA0A0R4IMS9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POLR2A at approximately 250 kDa. The expected band size for POLR2A is at 217 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-polr2b-antibody-aza0a8m1n6s0-boster.html2026-03-17T05:16:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1n6s0-polr2b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish POLR2B Antibody Picoband®Western blot analysis of POLR2B using anti-POLR2B antibody (AZA0A8M1N6S0). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2B antigen affinity purified polyclonal antibody (AZA0A8M1N6S0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POLR2B at approximately 130 kDa. The expected band size for POLR2B is at 134 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-polr2d-antibody-aza0a8m1n062-boster.html2026-03-17T05:16:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1n062-polr2d-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish POLR2D AntibodyIHC analysis of POLR2D using anti-POLR2D antibody (AZA0A8M1N062). POLR2D was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLR2D Antibody (AZA0A8M1N062) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1n062-polr2d-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish POLR2D AntibodyIHC analysis of POLR2D using anti-POLR2D antibody (AZA0A8M1N062). POLR2D was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLR2D Antibody (AZA0A8M1N062) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1n062-polr2d-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish POLR2D AntibodyIHC analysis of POLR2D using anti-POLR2D antibody (AZA0A8M1N062). POLR2D was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLR2D Antibody (AZA0A8M1N062) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ppm1g-antibody-azf1qje5-boster.html2026-03-17T05:16:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qje5-ppm1g-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PPM1G Antibody Picoband®Western blot analysis of PPM1G using anti-PPM1G antibody (AZF1QJE5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1G antigen affinity purified polyclonal antibody (AZF1QJE5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPM1G at approximately 70 kDa. The expected band size for PPM1G is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qje5-ppm1g-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PPM1G Antibody Picoband®IHC analysis of PPM1G using anti-PPM1G antibody (AZF1QJE5). PPM1G was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPM1G Antibody (AZF1QJE5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mypt1-ppp1r12a-antibody-azq6drg7-boster.html2026-03-17T05:16:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6drg7-ppp1r12a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MYPT1/PPP1R12A Antibody Picoband®Western blot analysis of PPP1R12A using anti-PPP1R12A antibody (AZQ6DRG7). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R12A antigen affinity purified polyclonal antibody (AZQ6DRG7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPP1R12A at approximately 140 kDa. The expected band size for PPP1R12A is at 115 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6drg7-ppp1r12a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish MYPT1/PPP1R12A Antibody Picoband®IHC analysis of PPP1R12A using anti-PPP1R12A antibody (AZQ6DRG7). PPP1R12A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP1R12A Antibody (AZQ6DRG7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6drg7-ppp1r12a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish MYPT1/PPP1R12A Antibody Picoband®IHC analysis of PPP1R12A using anti-PPP1R12A antibody (AZQ6DRG7). PPP1R12A was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP1R12A Antibody (AZQ6DRG7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6drg7-ppp1r12a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish MYPT1/PPP1R12A Antibody Picoband®IHC analysis of PPP1R12A using anti-PPP1R12A antibody (AZQ6DRG7). PPP1R12A was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP1R12A Antibody (AZQ6DRG7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ppp3r1a-b-antibody-aza0a8m3bef6-boster.html2026-03-17T05:16:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3bef6-ppp3r1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PPP3R1a/b Antibody Picoband®Western blot analysis of PPP3R1a/b using anti-PPP3R1a/b antibody (AZA0A8M3BEF6). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3R1a/b antigen affinity purified polyclonal antibody (AZA0A8M3BEF6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPP3R1a/b at approximately 15 kDa. The expected band size for PPP3R1a/b is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3bef6-ppp3r1a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PPP3R1a/b Antibody Picoband®IHC analysis of PPP3R1a/b using anti-PPP3R1a/b antibody (AZA0A8M3BEF6). PPP3R1a/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP3R1a/b Antibody (AZA0A8M3BEF6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3bef6-ppp3r1a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PPP3R1a/b Antibody Picoband®IHC analysis of PPP3R1a/b using anti-PPP3R1a/b antibody (AZA0A8M3BEF6). PPP3R1a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP3R1a/b Antibody (AZA0A8M3BEF6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3bef6-ppp3r1a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PPP3R1a/b Antibody Picoband®IHC analysis of PPP3R1a/b using anti-PPP3R1a/b antibody (AZA0A8M3BEF6). PPP3R1a/b was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP3R1a/b Antibody (AZA0A8M3BEF6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-prdx4-antibody-aza3kp44-boster.html2026-03-17T05:16:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza3kp44-prdx4-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PRDX4 Antibody Picoband®Western blot analysis of PRDX4 using anti-PRDX4 antibody (AZA3KP44). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX4 antigen affinity purified polyclonal antibody (AZA3KP44) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRDX4 at approximately 27 kDa. The expected band size for PRDX4 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza3kp44-prdx4-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PRDX4 Antibody Picoband®IHC analysis of PRDX4 using anti-PRDX4 antibody (AZA3KP44). PRDX4 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRDX4 Antibody (AZA3KP44) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza3kp44-prdx4-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PRDX4 Antibody Picoband®IHC analysis of PRDX4 using anti-PRDX4 antibody (AZA3KP44). PRDX4 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRDX4 Antibody (AZA3KP44) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-prox1a-antibody-azf1qae1-boster.html2026-03-17T05:16:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qae1-prox1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PROX1a Antibody Picoband®Western blot analysis of PROX1a using anti-PROX1a antibody (AZF1QAE1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROX1a antigen affinity purified polyclonal antibody (AZF1QAE1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PROX1a at approximately 95 kDa. The expected band size for PROX1a is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qae1-prox1a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PROX1a Antibody Picoband®IHC analysis of PROX1a using anti-PROX1a antibody (AZF1QAE1). PROX1a was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PROX1a Antibody (AZF1QAE1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-prpf18-antibody-azq6gmh0-boster.html2026-03-17T05:16:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6gmh0-prpf18-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PRPF18 Antibody Picoband®Western blot analysis of PRPF18 using anti-PRPF18 antibody (AZQ6GMH0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF18 antigen affinity purified polyclonal antibody (AZQ6GMH0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRPF18 at approximately 40 kDa. The expected band size for PRPF18 is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-prpf31-antibody-azq7sxm7-boster.html2026-03-17T05:16:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxm7-prpf31-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PRPF31 Antibody Picoband®Western blot analysis of PRPF31 using anti-PRPF31 antibody (AZQ7SXM7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF31 antigen affinity purified polyclonal antibody (AZQ7SXM7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRPF31 at approximately 55 kDa. The expected band size for PRPF31 is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-prpf6-antibody-aza0a2r8pwg5-boster.html2026-03-17T05:16:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8pwg5-prpf6-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PRPF6 Antibody Picoband®Western blot analysis of PRPF6 using anti-PRPF6 antibody (AZA0A2R8PWG5). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF6 antigen affinity purified polyclonal antibody (AZA0A2R8PWG5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRPF6 at approximately 107 kDa. The expected band size for PRPF6 is at 107 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8pwg5-prpf6-primary-antibodies-if-testing-1.jpgAnti-Zebrafish PRPF6 Antibody Picoband®IF analysis of PRPF6 using anti-PRPF6 antibody (AZA0A2R8PWG5). PRPF6 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PRPF6 Antibody (AZA0A2R8PWG5) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psma1-antibody-azq6dgx8-boster.html2026-03-17T05:16:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dgx8-psma1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PSMA1 Antibody Picoband®Western blot analysis of PSMA1 using anti-PSMA1 antibody (AZQ6DGX8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA1 antigen affinity purified polyclonal antibody (AZQ6DGX8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMA1 at approximately 33 kDa. The expected band size for PSMA1 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dgx8-psma1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PSMA1 Antibody Picoband®IHC analysis of PSMA1 using anti-PSMA1 antibody (AZQ6DGX8). PSMA1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA1 Antibody (AZQ6DGX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dgx8-psma1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PSMA1 Antibody Picoband®IHC analysis of PSMA1 using anti-PSMA1 antibody (AZQ6DGX8). PSMA1 was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA1 Antibody (AZQ6DGX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dgx8-psma1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PSMA1 Antibody Picoband®IHC analysis of PSMA1 using anti-PSMA1 antibody (AZQ6DGX8). PSMA1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA1 Antibody (AZQ6DGX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dgx8-psma1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish PSMA1 Antibody Picoband®IHC analysis of PSMA1 using anti-PSMA1 antibody (AZQ6DGX8). PSMA1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA1 Antibody (AZQ6DGX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dgx8-psma1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish PSMA1 Antibody Picoband®IHC analysis of PSMA1 using anti-PSMA1 antibody (AZQ6DGX8). PSMA1 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA1 Antibody (AZQ6DGX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psma3-antibody-azq4v918-boster.html2026-03-17T05:16:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq4v918-psma3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PSMA3 Antibody Picoband®Western blot analysis of PSMA3 using anti-PSMA3 antibody (AZQ4V918). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA3 antigen affinity purified polyclonal antibody (AZQ4V918) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMA3 at approximately 28 kDa. The expected band size for PSMA3 is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psma5-antibody-azq6tgv6-boster.html2026-03-17T05:16:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tgv6-psma5-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PSMA5 Antibody Picoband®Western blot analysis of PSMA5 using anti-PSMA5 antibody (AZQ6TGV6). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA5 antigen affinity purified polyclonal antibody (AZQ6TGV6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMA5 at approximately 26 kDa. The expected band size for PSMA5 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tgv6-psma5-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PSMA5 Antibody Picoband®IHC analysis of PSMA5 using anti-PSMA5 antibody (AZQ6TGV6). PSMA5 was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA5 Antibody (AZQ6TGV6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tgv6-psma5-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PSMA5 Antibody Picoband®IHC analysis of PSMA5 using anti-PSMA5 antibody (AZQ6TGV6). PSMA5 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA5 Antibody (AZQ6TGV6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psmb1-antibody-azq6drf3-boster.html2026-03-17T05:16:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6drf3-psmb1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PSMB1 Antibody Picoband®Western blot analysis of PSMB1 using anti-PSMB1 antibody (AZQ6DRF3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB1 antigen affinity purified polyclonal antibody (AZQ6DRF3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMB1 at approximately 24 kDa. The expected band size for PSMB1 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6drf3-psmb1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PSMB1 Antibody Picoband®IHC analysis of PSMB1 using anti-PSMB1 antibody (AZQ6DRF3). PSMB1 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMB1 Antibody (AZQ6DRF3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psmc1a-b-antibody-azq7zwd1-boster.html2026-03-17T05:16:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zwd1-psmc1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PSMC1a/b Antibody Picoband®Western blot analysis of PSMC1a/b using anti-PSMC1a/b antibody (AZQ7ZWD1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC1a/b antigen affinity purified polyclonal antibody (AZQ7ZWD1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMC1a/b at approximately 57 kDa. The expected band size for PSMC1a/b is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zwd1-psmc1a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PSMC1a/b Antibody Picoband®IHC analysis of PSMC1a/b using anti-PSMC1a/b antibody (AZQ7ZWD1). PSMC1a/b was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC1a/b Antibody (AZQ7ZWD1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zwd1-psmc1a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PSMC1a/b Antibody Picoband®IHC analysis of PSMC1a/b using anti-PSMC1a/b antibody (AZQ7ZWD1). PSMC1a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC1a/b Antibody (AZQ7ZWD1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zwd1-psmc1a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PSMC1a/b Antibody Picoband®IHC analysis of PSMC1a/b using anti-PSMC1a/b antibody (AZQ7ZWD1). PSMC1a/b was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC1a/b Antibody (AZQ7ZWD1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zwd1-psmc1a-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish PSMC1a/b Antibody Picoband®IHC analysis of PSMC1a/b using anti-PSMC1a/b antibody (AZQ7ZWD1). PSMC1a/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC1a/b Antibody (AZQ7ZWD1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zwd1-psmc1a-b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish PSMC1a/b Antibody Picoband®IHC analysis of PSMC1a/b using anti-PSMC1a/b antibody (AZQ7ZWD1). PSMC1a/b was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC1a/b Antibody (AZQ7ZWD1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zwd1-psmc1a-b-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish PSMC1a/b Antibody Picoband®IHC analysis of PSMC1a/b using anti-PSMC1a/b antibody (AZQ7ZWD1). PSMC1a/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC1a/b Antibody (AZQ7ZWD1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zwd1-psmc1a-b-primary-antibodies-ihc-testing-7.jpgAnti-Zebrafish PSMC1a/b Antibody Picoband®IHC analysis of PSMC1a/b using anti-PSMC1a/b antibody (AZQ7ZWD1). PSMC1a/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC1a/b Antibody (AZQ7ZWD1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psmc2-antibody-azq7t3b1-boster.html2026-03-17T05:16:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t3b1-psmc2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PSMC2 Antibody Picoband®Western blot analysis of PSMC2 using anti-PSMC2 antibody (AZQ7T3B1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC2 antigen affinity purified polyclonal antibody (AZQ7T3B1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMC2 at approximately 49 kDa. The expected band size for PSMC2 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t3b1-psmc2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PSMC2 Antibody Picoband®IHC analysis of PSMC2 using anti-PSMC2 antibody (AZQ7T3B1). PSMC2 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC2 Antibody (AZQ7T3B1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t3b1-psmc2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PSMC2 Antibody Picoband®IHC analysis of PSMC2 using anti-PSMC2 antibody (AZQ7T3B1). PSMC2 was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC2 Antibody (AZQ7T3B1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t3b1-psmc2-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PSMC2 Antibody Picoband®IHC analysis of PSMC2 using anti-PSMC2 antibody (AZQ7T3B1). PSMC2 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC2 Antibody (AZQ7T3B1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t3b1-psmc2-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish PSMC2 Antibody Picoband®IHC analysis of PSMC2 using anti-PSMC2 antibody (AZQ7T3B1). PSMC2 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC2 Antibody (AZQ7T3B1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tbp-1-psmc3-antibody-aza0a0r4ila8-boster.html2026-03-17T05:16:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4ila8-psmc3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TBP-1/PSMC3 Antibody Picoband®Western blot analysis of PSMC3 using anti-PSMC3 antibody (AZA0A0R4ILA8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC3 antigen affinity purified polyclonal antibody (AZA0A0R4ILA8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMC3 at approximately 49 kDa. The expected band size for PSMC3 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4ila8-psmc3-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish TBP-1/PSMC3 Antibody Picoband®IHC analysis of PSMC3 using anti-PSMC3 antibody (AZA0A0R4ILA8). PSMC3 was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC3 Antibody (AZA0A0R4ILA8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4ila8-psmc3-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish TBP-1/PSMC3 Antibody Picoband®IHC analysis of PSMC3 using anti-PSMC3 antibody (AZA0A0R4ILA8). PSMC3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC3 Antibody (AZA0A0R4ILA8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4ila8-psmc3-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish TBP-1/PSMC3 Antibody Picoband®IHC analysis of PSMC3 using anti-PSMC3 antibody (AZA0A0R4ILA8). PSMC3 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC3 Antibody (AZA0A0R4ILA8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psmc5-antibody-azq6azc1-boster.html2026-03-17T05:16:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azc1-psmc5-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PSMC5 AntibodyIHC analysis of PSMC5 using anti-PSMC5 antibody (AZQ6AZC1). PSMC5 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC5 Antibody (AZQ6AZC1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azc1-psmc5-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PSMC5 AntibodyIHC analysis of PSMC5 using anti-PSMC5 antibody (AZQ6AZC1). PSMC5 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC5 Antibody (AZQ6AZC1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azc1-psmc5-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PSMC5 AntibodyIHC analysis of PSMC5 using anti-PSMC5 antibody (AZQ6AZC1). PSMC5 was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC5 Antibody (AZQ6AZC1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azc1-psmc5-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish PSMC5 AntibodyIHC analysis of PSMC5 using anti-PSMC5 antibody (AZQ6AZC1). PSMC5 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC5 Antibody (AZQ6AZC1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azc1-psmc5-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish PSMC5 AntibodyIHC analysis of PSMC5 using anti-PSMC5 antibody (AZQ6AZC1). PSMC5 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC5 Antibody (AZQ6AZC1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azc1-psmc5-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish PSMC5 AntibodyIHC analysis of PSMC5 using anti-PSMC5 antibody (AZQ6AZC1). PSMC5 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC5 Antibody (AZQ6AZC1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6azc1-psmc5-primary-antibodies-ihc-testing-7.jpgAnti-Zebrafish PSMC5 AntibodyIHC analysis of PSMC5 using anti-PSMC5 antibody (AZQ6AZC1). PSMC5 was detected in a paraffin-embedded section of zebrafish bone tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMC5 Antibody (AZQ6AZC1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psmd13-antibody-azq7zu69-boster.html2026-03-17T05:16:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zu69-psmd13-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PSMD13 Antibody Picoband®Western blot analysis of PSMD13 using anti-PSMD13 antibody (AZQ7ZU69). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD13 antigen affinity purified polyclonal antibody (AZQ7ZU69) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMD13 at approximately 39 kDa. The expected band size for PSMD13 is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-poh1-psmd14-antibody-aza3knn5-boster.html2026-03-17T05:16:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza3knn5-psmd14-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish POH1/PSMD14 Antibody Picoband®Western blot analysis of PSMD14 using anti-PSMD14 antibody (AZA3KNN5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD14 antigen affinity purified polyclonal antibody (AZA3KNN5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMD14 at approximately 35 kDa. The expected band size for PSMD14 is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psmd2-antibody-azq6phk7-boster.html2026-03-17T05:16:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phk7-psmd2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PSMD2 Antibody Picoband®Western blot analysis of PSMD2 using anti-PSMD2 antibody (AZQ6PHK7). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD2 antigen affinity purified polyclonal antibody (AZQ6PHK7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMD2 at approximately 97 kDa. The expected band size for PSMD2 is at 100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phk7-psmd2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PSMD2 Antibody Picoband®IHC analysis of PSMD2 using anti-PSMD2 antibody (AZQ6PHK7). PSMD2 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD2 Antibody (AZQ6PHK7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phk7-psmd2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PSMD2 Antibody Picoband®IHC analysis of PSMD2 using anti-PSMD2 antibody (AZQ6PHK7). PSMD2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD2 Antibody (AZQ6PHK7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phk7-psmd2-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PSMD2 Antibody Picoband®IHC analysis of PSMD2 using anti-PSMD2 antibody (AZQ6PHK7). PSMD2 was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD2 Antibody (AZQ6PHK7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psmd4a-b-antibody-azq6iqc6-boster.html2026-03-17T05:16:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqc6-psmd4a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PSMD4a/b Antibody Picoband®Western blot analysis of PSMD4a/b using anti-PSMD4a/b antibody (AZQ6IQC6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD4a/b antigen affinity purified polyclonal antibody (AZQ6IQC6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMD4a/b at approximately 50 kDa. The expected band size for PSMD4a/b is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psmd6-antibody-azq7zwe5-boster.html2026-03-17T05:16:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zwe5-psmd6-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PSMD6 Antibody Picoband®Western blot analysis of PSMD6 using anti-PSMD6 antibody (AZQ7ZWE5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD6 antigen affinity purified polyclonal antibody (AZQ7ZWE5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMD6 at approximately 46 kDa. The expected band size for PSMD6 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zwe5-psmd6-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PSMD6 Antibody Picoband®IHC analysis of PSMD6 using anti-PSMD6 antibody (AZQ7ZWE5). PSMD6 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD6 Antibody (AZQ7ZWE5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zwe5-psmd6-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PSMD6 Antibody Picoband®IHC analysis of PSMD6 using anti-PSMD6 antibody (AZQ7ZWE5). PSMD6 was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD6 Antibody (AZQ7ZWE5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psmd7-antibody-azq7zyx7-boster.html2026-03-17T05:16:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zyx7-psmd7-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PSMD7 Antibody Picoband®Western blot analysis of PSMD7 using anti-PSMD7 antibody (AZQ7ZYX7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD7 antigen affinity purified polyclonal antibody (AZQ7ZYX7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMD7 at approximately 38 kDa. The expected band size for PSMD7 is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-psmd8-antibody-azq6iqh4-boster.html2026-03-17T05:16:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqh4-psmd8-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PSMD8 AntibodyIHC analysis of PSMD8 using anti-PSMD8 antibody (AZQ6IQH4). PSMD8 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD8 Antibody (AZQ6IQH4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqh4-psmd8-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PSMD8 AntibodyIHC analysis of PSMD8 using anti-PSMD8 antibody (AZQ6IQH4). PSMD8 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD8 Antibody (AZQ6IQH4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqh4-psmd8-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PSMD8 AntibodyIHC analysis of PSMD8 using anti-PSMD8 antibody (AZQ6IQH4). PSMD8 was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD8 Antibody (AZQ6IQH4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqh4-psmd8-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish PSMD8 AntibodyIHC analysis of PSMD8 using anti-PSMD8 antibody (AZQ6IQH4). PSMD8 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD8 Antibody (AZQ6IQH4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rab1aa-ab-antibody-azb8jlc8-boster.html2026-03-17T05:16:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jlc8-rab1aa-ab-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RAB1aa/ab Antibody Picoband®Western blot analysis of RAB1aa/ab using anti-RAB1aa/ab antibody (AZB8JLC8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB1aa/ab antigen affinity purified polyclonal antibody (AZB8JLC8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB1aa/ab at approximately 23 kDa. The expected band size for RAB1aa/ab is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jlc8-rab1aa-ab-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish RAB1aa/ab Antibody Picoband®IHC analysis of RAB1aa/ab using anti-RAB1aa/ab antibody (AZB8JLC8). RAB1aa/ab was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB1aa/ab Antibody (AZB8JLC8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jlc8-rab1aa-ab-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish RAB1aa/ab Antibody Picoband®IHC analysis of RAB1aa/ab using anti-RAB1aa/ab antibody (AZB8JLC8). RAB1aa/ab was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB1aa/ab Antibody (AZB8JLC8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rab14-4l-antibody-azq7zvx4-boster.html2026-03-17T05:16:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvx4-rab14-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RAB14/4l Antibody Picoband®Western blot analysis of RAB14 using anti-RAB14 antibody (AZQ7ZVX4). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB14 antigen affinity purified polyclonal antibody (AZQ7ZVX4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB14 at approximately 24 kDa. The expected band size for RAB14 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvx4-rab14-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish RAB14/4l Antibody Picoband®IHC analysis of RAB14 using anti-RAB14 antibody (AZQ7ZVX4). RAB14 was detected in a paraffin-embedded section of zebrafish spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB14 Antibody (AZQ7ZVX4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvx4-rab14-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish RAB14/4l Antibody Picoband®IHC analysis of RAB14 using anti-RAB14 antibody (AZQ7ZVX4). RAB14 was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB14 Antibody (AZQ7ZVX4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rab4aa-antibody-azq6phi9-boster.html2026-03-17T05:16:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6phi9-rab4a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RAB4Aa Antibody Picoband®Western blot analysis of RAB4a using anti-RAB4a antibody (AZQ6PHI9). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB4a antigen affinity purified polyclonal antibody (AZQ6PHI9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB4a at approximately 24 kDa. The expected band size for RAB4a is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rab5aa-ab-antibody-azq7zuw5-boster.html2026-03-17T05:16:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zuw5-rab5aa-ab-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RAB5aa/ab Antibody Picoband®Western blot analysis of RAB5aa/ab using anti-RAB5aa/ab antibody (AZQ7ZUW5). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB5aa/ab antigen affinity purified polyclonal antibody (AZQ7ZUW5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB5aa/ab at approximately 24 kDa. The expected band size for RAB5aa/ab is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-leo1-antibody-azq6nyv9-boster.html2026-03-17T05:16:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nyv9-leo1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LEO1 Antibody Picoband®Western blot analysis of LEO1 using anti-LEO1 antibody (AZQ6NYV9). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LEO1 antigen affinity purified polyclonal antibody (AZQ6NYV9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LEO1 at approximately 105 kDa. The expected band size for LEO1 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nyv9-leo1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish LEO1 Antibody Picoband®IHC analysis of LEO1 using anti-LEO1 antibody (AZQ6NYV9). LEO1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LEO1 Antibody (AZQ6NYV9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nyv9-leo1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish LEO1 Antibody Picoband®IHC analysis of LEO1 using anti-LEO1 antibody (AZQ6NYV9). LEO1 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LEO1 Antibody (AZQ6NYV9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nyv9-leo1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish LEO1 Antibody Picoband®IHC analysis of LEO1 using anti-LEO1 antibody (AZQ6NYV9). LEO1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LEO1 Antibody (AZQ6NYV9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nyv9-leo1-primary-antibodies-if-testing-1.jpgAnti-Zebrafish LEO1 Antibody Picoband®IF analysis of LEO1 using anti-LEO1 antibody (AZQ6NYV9). LEO1 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-LEO1 Antibody (AZQ6NYV9) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nyv9-leo1-primary-antibodies-if-testing-2_1.jpgAnti-Zebrafish LEO1 Antibody Picoband®IF analysis of LEO1 using anti-LEO1 antibody (AZQ6NYV9). LEO1 was detected in a paraffin-embedded section of zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-LEO1 Antibody (AZQ6NYV9) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nyv9-leo1-primary-antibodies-if-testing-3_1.jpgAnti-Zebrafish LEO1 Antibody Picoband®IF analysis of LEO1 using anti-LEO1 antibody (AZQ6NYV9). LEO1 was detected in a paraffin-embedded section of zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-LEO1 Antibody (AZQ6NYV9) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dgcr8-antibody-aza2beg3-boster.html2026-03-17T05:16:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2beg3-dgcr8-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish DGCR8 AntibodyIHC analysis of DGCR8 using anti-DGCR8 antibody (AZA2BEG3). DGCR8 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DGCR8 Antibody (AZA2BEG3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2beg3-dgcr8-primary-antibodies-if-testing-1.jpgAnti-Zebrafish DGCR8 AntibodyIF analysis of DGCR8 using anti-DGCR8 antibody (AZA2BEG3). DGCR8 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DGCR8 Antibody (AZA2BEG3) overnight at 4°C. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2beg3-dgcr8-primary-antibodies-if-testing-2.jpgAnti-Zebrafish DGCR8 AntibodyIF analysis of DGCR8 using anti-DGCR8 antibody (AZA2BEG3). DGCR8 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DGCR8 Antibody (AZA2BEG3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dnm1a-b-antibody-aza0a8m2bg30-boster.html2026-03-17T05:16:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bg30-dnm1a-b-primary-antibodies-if-testing-1.jpgAnti-Zebrafish DNM1a/b AntibodyIF analysis of DNM1a/b using anti-DNM1a/b antibody (AZA0A8M2BG30). DNM1a/b was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DNM1a/b Antibody (AZA0A8M2BG30) overnight at 4°C. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bg30-dnm1a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish DNM1a/b AntibodyIHC analysis of DNM1a/b using anti-DNM1a/b antibody (AZA0A8M2BG30). DNM1a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNM1a/b Antibody (AZA0A8M2BG30) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bg30-dnm1a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish DNM1a/b AntibodyIHC analysis of DNM1a/b using anti-DNM1a/b antibody (AZA0A8M2BG30). DNM1a/b was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNM1a/b Antibody (AZA0A8M2BG30) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rbap48-rbbp4-antibody-azq6p3h7-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p3h7-rbap48-rbbp4-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RBAP48/RBBP4 Antibody Picoband®Western blot analysis of RBAP48/RBBP4 using anti-RBAP48/RBBP4 antibody (AZQ6P3H7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBAP48/RBBP4 antigen affinity purified polyclonal antibody (AZQ6P3H7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBAP48/RBBP4 at approximately 55 kDa. The expected band size for RBAP48/RBBP4 is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rbm22-antibody-azq6nzz9-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nzz9-rbm22-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RBM22 Antibody Picoband®Western blot analysis of RBM22 using anti-RBM22 antibody (AZQ6NZZ9). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM22 antigen affinity purified polyclonal antibody (AZQ6NZZ9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBM22 at approximately 47 kDa. The expected band size for RBM22 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rcc2-antibody-azq6nye2-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nye2-rcc2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RCC2 Antibody Picoband®Western blot analysis of RCC2 using anti-RCC2 antibody (AZQ6NYE2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates, Lane 3: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RCC2 antigen affinity purified polyclonal antibody (AZQ6NYE2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RCC2 at approximately 60 kDa. The expected band size for RCC2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nye2-rcc2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish RCC2 Antibody Picoband®IHC analysis of RCC2 using anti-RCC2 antibody (AZQ6NYE2). RCC2 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RCC2 Antibody (AZQ6NYE2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nye2-rcc2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish RCC2 Antibody Picoband®IHC analysis of RCC2 using anti-RCC2 antibody (AZQ6NYE2). RCC2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RCC2 Antibody (AZQ6NYE2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nye2-rcc2-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish RCC2 Antibody Picoband®IHC analysis of RCC2 using anti-RCC2 antibody (AZQ6NYE2). RCC2 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RCC2 Antibody (AZQ6NYE2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nye2-rcc2-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish RCC2 Antibody Picoband®IHC analysis of RCC2 using anti-RCC2 antibody (AZQ6NYE2). RCC2 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RCC2 Antibody (AZQ6NYE2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sars-sars1-antibody-azq6drc0-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6drc0-sars-sars1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SARS/SARS1 Antibody Picoband®Western blot analysis of SARS/SARS1 using anti-SARS/SARS1 antibody (AZQ6DRC0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SARS/SARS1 antigen affinity purified polyclonal antibody (AZQ6DRC0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SARS/SARS1 at approximately 64 kDa. The expected band size for SARS/SARS1 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6drc0-sars-sars1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish SARS/SARS1 Antibody Picoband®IHC analysis of SARS/SARS1 using anti-SARS/SARS1 antibody (AZQ6DRC0). SARS/SARS1 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SARS/SARS1 Antibody (AZQ6DRC0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sdha-antibody-azq7zvf3-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvf3-sdha-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SDHA Antibody Picoband®Western blot analysis of SDHA using anti-SDHA antibody (AZQ7ZVF3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDHA antigen affinity purified polyclonal antibody (AZQ7ZVF3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SDHA at approximately 72 kDa. The expected band size for SDHA is at 72 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-septin7a-b-antibody-aza0a096x788-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a096x788-septin7a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SEPTIN7A/B Antibody Picoband®Western blot analysis of SEPTIN7A/B using anti-SEPTIN7A/B antibody (AZA0A096X788). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPTIN7A/B antigen affinity purified polyclonal antibody (AZA0A096X788) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SEPTIN7A/B at approximately 50 kDa. The expected band size for SEPTIN7A/B is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sf3a1-antibody-azq90x41-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90x41-sf3a1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish SF3A1 AntibodyIHC analysis of SF3A1 using anti-SF3A1 antibody (AZQ90X41). SF3A1 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (AZQ90X41) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90x41-sf3a1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish SF3A1 AntibodyIHC analysis of SF3A1 using anti-SF3A1 antibody (AZQ90X41). SF3A1 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (AZQ90X41) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90x41-sf3a1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish SF3A1 AntibodyIHC analysis of SF3A1 using anti-SF3A1 antibody (AZQ90X41). SF3A1 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (AZQ90X41) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90x41-sf3a1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish SF3A1 AntibodyIHC analysis of SF3A1 using anti-SF3A1 antibody (AZQ90X41). SF3A1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (AZQ90X41) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90x41-sf3a1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish SF3A1 AntibodyIHC analysis of SF3A1 using anti-SF3A1 antibody (AZQ90X41). SF3A1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (AZQ90X41) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90x41-sf3a1-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish SF3A1 AntibodyIHC analysis of SF3A1 using anti-SF3A1 antibody (AZQ90X41). SF3A1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3A1 Antibody (AZQ90X41) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90x41-sf3a1-primary-antibodies-if-testing-1.jpgAnti-Zebrafish SF3A1 AntibodyIF analysis of SF3A1 using anti-SF3A1 antibody (AZQ90X41). SF3A1 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SF3A1 Antibody (AZQ90X41) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sf3b3-antibody-azq1lve8-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1lve8-sf3b3-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish SF3B3 AntibodyIHC analysis of SF3B3 using anti-SF3B3 antibody (AZQ1LVE8). SF3B3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (AZQ1LVE8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1lve8-sf3b3-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish SF3B3 AntibodyIHC analysis of SF3B3 using anti-SF3B3 antibody (AZQ1LVE8). SF3B3 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (AZQ1LVE8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1lve8-sf3b3-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish SF3B3 AntibodyIHC analysis of SF3B3 using anti-SF3B3 antibody (AZQ1LVE8). SF3B3 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (AZQ1LVE8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1lve8-sf3b3-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish SF3B3 AntibodyIHC analysis of SF3B3 using anti-SF3B3 antibody (AZQ1LVE8). SF3B3 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF3B3 Antibody (AZQ1LVE8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1lve8-sf3b3-primary-antibodies-if-testing-1.jpgAnti-Zebrafish SF3B3 AntibodyIF analysis of SF3B3 using anti-SF3B3 antibody (AZQ1LVE8). SF3B3 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SF3B3 Antibody (AZQ1LVE8) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-smarca4a-antibody-azq7zsy3-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zsy3-smarca4a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SMARCA4A Antibody Picoband®Western blot analysis of SMARCA4A using anti-SMARCA4A antibody (AZQ7ZSY3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCA4A antigen affinity purified polyclonal antibody (AZQ7ZSY3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMARCA4A at approximately 220 kDa. The expected band size for SMARCA4A is at 185 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zsy3-smarca4a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish SMARCA4A Antibody Picoband®IHC analysis of SMARCA4A using anti-SMARCA4A antibody (AZQ7ZSY3). SMARCA4A was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA4A Antibody (AZQ7ZSY3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zsy3-smarca4a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish SMARCA4A Antibody Picoband®IHC analysis of SMARCA4A using anti-SMARCA4A antibody (AZQ7ZSY3). SMARCA4A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA4A Antibody (AZQ7ZSY3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zsy3-smarca4a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish SMARCA4A Antibody Picoband®IHC analysis of SMARCA4A using anti-SMARCA4A antibody (AZQ7ZSY3). SMARCA4A was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCA4A Antibody (AZQ7ZSY3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zsy3-smarca4a-primary-antibodies-if-testing-1.jpgAnti-Zebrafish SMARCA4A Antibody Picoband®IF analysis of SMARCA4A using anti-SMARCA4A antibody (AZQ7ZSY3). SMARCA4A was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SMARCA4A Antibody (AZQ7ZSY3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-srp54-antibody-azq7zvn5-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvn5-srp54-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SRP54 Antibody Picoband®Western blot analysis of SRP54 using anti-SRP54 antibody (AZQ7ZVN5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRP54 antigen affinity purified polyclonal antibody (AZQ7ZVN5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SRP54 at approximately 56 kDa. The expected band size for SRP54 is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-twist1a-1b-2-antibody-azq9pte3-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pte3-twist1a-1b-2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TWIST1A/1B/2 Antibody Picoband®Western blot analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody (AZQ9PTE3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWIST1A/1B/2 antigen affinity purified polyclonal antibody (AZQ9PTE3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TWIST1A/1B/2 at approximately 28 kDa. The expected band size for TWIST1A/1B/2 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pte3-twist1a-1b-2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish TWIST1A/1B/2 Antibody Picoband®IHC analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody (AZQ9PTE3). TWIST1A/1B/2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TWIST1A/1B/2 Antibody (AZQ9PTE3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pte3-twist1a-1b-2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish TWIST1A/1B/2 Antibody Picoband®IHC analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody (AZQ9PTE3). TWIST1A/1B/2 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TWIST1A/1B/2 Antibody (AZQ9PTE3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pte3-twist1a-1b-2-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish TWIST1A/1B/2 Antibody Picoband®IHC analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody (AZQ9PTE3). TWIST1A/1B/2 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TWIST1A/1B/2 Antibody (AZQ9PTE3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pte3-twist1a-1b-2-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish TWIST1A/1B/2 Antibody Picoband®IHC analysis of TWIST1A/1B/2 using anti-TWIST1A/1B/2 antibody (AZQ9PTE3). TWIST1A/1B/2 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TWIST1A/1B/2 Antibody (AZQ9PTE3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-vcp-antibody-azq7zu99-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zu99-vcp-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish VCP Antibody Picoband®Western blot analysis of VCP using anti-VCP antibody (AZQ7ZU99). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (AZQ7ZU99) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VCP at approximately 97 kDa. The expected band size for VCP is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zu99-vcp-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish VCP Antibody Picoband®IHC analysis of VCP using anti-VCP antibody (AZQ7ZU99). VCP was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCP Antibody (AZQ7ZU99) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zu99-vcp-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish VCP Antibody Picoband®IHC analysis of VCP using anti-VCP antibody (AZQ7ZU99). VCP was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCP Antibody (AZQ7ZU99) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zu99-vcp-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish VCP Antibody Picoband®IHC analysis of VCP using anti-VCP antibody (AZQ7ZU99). VCP was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCP Antibody (AZQ7ZU99) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zu99-vcp-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish VCP Antibody Picoband®IHC analysis of VCP using anti-VCP antibody (AZQ7ZU99). VCP was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCP Antibody (AZQ7ZU99) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zu99-vcp-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish VCP Antibody Picoband®IHC analysis of VCP using anti-VCP antibody (AZQ7ZU99). VCP was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCP Antibody (AZQ7ZU99) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zu99-vcp-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish VCP Antibody Picoband®IHC analysis of VCP using anti-VCP antibody (AZQ7ZU99). VCP was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VCP Antibody (AZQ7ZU99) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-xpo1a-b-antibody-aze7f0e8-boster.html2026-03-17T05:16:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f0e8-xpo1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish XPO1A/B Antibody Picoband®Western blot analysis of XPO1A/B using anti-XPO1A/B antibody (AZE7F0E8). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XPO1A/B antigen affinity purified polyclonal antibody (AZE7F0E8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for XPO1A/B at approximately 110 kDa. The expected band size for XPO1A/B is at 123 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-kinase-p110-beta-pik3cb-antibody-aze7f251-boster.html2026-03-17T05:16:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f251-kinase-p110-beta-pik3cb-primary-antibodies-if-testing-1.jpgAnti-Zebrafish Kinase p110 Beta/PIK3CB AntibodyIF analysis of Kinase p110 Beta/PIK3CB using anti-Kinase p110 Beta/PIK3CB antibody (AZE7F251). Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Kinase p110 Beta/PIK3CB Antibody (AZE7F251) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f251-kinase-p110-beta-pik3cb-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Kinase p110 Beta/PIK3CB AntibodyIHC analysis of Kinase p110 Beta/PIK3CB using anti-Kinase p110 Beta/PIK3CB antibody (AZE7F251). Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kinase p110 Beta/PIK3CB Antibody (AZE7F251) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f251-kinase-p110-beta-pik3cb-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Kinase p110 Beta/PIK3CB AntibodyIHC analysis of Kinase p110 Beta/PIK3CB using anti-Kinase p110 Beta/PIK3CB antibody (AZE7F251). Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kinase p110 Beta/PIK3CB Antibody (AZE7F251) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f251-kinase-p110-beta-pik3cb-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish Kinase p110 Beta/PIK3CB AntibodyIHC analysis of Kinase p110 Beta/PIK3CB using anti-Kinase p110 Beta/PIK3CB antibody (AZE7F251). Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kinase p110 Beta/PIK3CB Antibody (AZE7F251) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f251-kinase-p110-beta-pik3cb-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish Kinase p110 Beta/PIK3CB AntibodyIHC analysis of Kinase p110 Beta/PIK3CB using anti-Kinase p110 Beta/PIK3CB antibody (AZE7F251). Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kinase p110 Beta/PIK3CB Antibody (AZE7F251) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f251-kinase-p110-beta-pik3cb-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish Kinase p110 Beta/PIK3CB AntibodyIHC analysis of Kinase p110 Beta/PIK3CB using anti-Kinase p110 Beta/PIK3CB antibody (AZE7F251). Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kinase p110 Beta/PIK3CB Antibody (AZE7F251) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f251-kinase-p110-beta-pik3cb-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish Kinase p110 Beta/PIK3CB AntibodyIHC analysis of Kinase p110 Beta/PIK3CB using anti-Kinase p110 Beta/PIK3CB antibody (AZE7F251). Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kinase p110 Beta/PIK3CB Antibody (AZE7F251) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1299982026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1299992026-03-13T05:05:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300002026-03-13T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00657-5-havcr2-primary-antibodies-wb-testing-1.jpgAnti-TIM3/HAVCR2 AntibodyWestern blot analysis of TIM3/HAVCR2 using anti-TIM3/HAVCR2 antibody (A00657-5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM3/HAVCR2 antigen affinity purified polyclonal antibody (A00657-5) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TIM3/HAVCR2 at approximately 70 kDa. The expected band size for TIM3/HAVCR2 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00657-5-fimmu-16-1681887-g012.jpgAnti-TIM3/HAVCR2 AntibodyThe knockdown of MFSD12 inhibited the proliferation, migration, and invasion of LIHC cells, as well as the TIM-3/Galectin-9 signaling pathway. (A, B) RT-qPCR and Western blot validation of MFSD12 silencing efficiency using siRNAs (si-MFSD12–1 to −4) with GAPDH as loading control. (C) CCK-8 cell viability assay showing reduced HEP 3B2.1–7 cells proliferation after MFSD12 knockdown (si-MFSD12-3). (D) Transwell assay revealed a reduction in the migratory and invasive capabilities of HEP 3B2.1–7 cells following the knockdown of MFSD12. (E) Immunoblot analysis of EMT markers and TIM-3 axis components showing up-regulation of E-cadherin and down-regulation of Vimentin, MMP-2, MMP-9, HAVCR2 (TIM-3) and LGALS9 in si-MFSD12-treated cells. * P < 0.05, ** P < 0.01, *** P < 0.001. CTRL, control untreated; si-NC, negative control siRNA; si-MFSD12, MFSD12-targeting siRNA; E-cadherin, epithelial cadherin; MMP-2/9, matrix metalloproteinase-2/9; HAVCR2, hepatitis A virus cellular receptor 2 (TIM-3); LGALS9, lectin galactoside-binding soluble 9 (Galectin-9). Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12582970/'>41194934</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00657-5-fimmu-16-1682724-g009.jpgAnti-TIM3/HAVCR2 AntibodyThe knockdown of CHMP4A inhibited the proliferation, migration, and invasion of LIHC cells, as well as the TIM-3/Galectin-9 signaling pathway. (A, B) RT-qPCR and Western blot validation of CHMP4A silencing efficiency using siRNAs (si-CHMP4A-1 to -4) with GAPDH as a loading control. (C) CCK-8 cell viability assay showing reduced proliferation of HEP 3B2.1–7 cells after CHMP4A knockdown (si-CHMP4A-2). (D) Transwell assay revealing a reduction in the migratory and invasive capabilities of HEP 3B2.1–7 cells following the knockdown of CHMP4A. (E) Immunoblot analysis of EMT markers and TIM-3 axis components showing upregulation of E-cadherin and downregulation of Vimentin, MMP-2, and MMP-9 in si-CHMP4A-treated cells. (F) Immunoblot analysis of TIM-3 axis components showing downregulation of HAVCR2 (TIM-3) and LGALS9 in si-CHMP4A-treated cells. ** P <0.01, *** P <0.001. CTRL, control untreated; si-NC, negative control siRNA; si-CHMP4A, CHMP4A-targeting siRNA; E-cadherin, epithelial cadherin; MMP-2/9, matrix metalloproteinase-2/9; HAVCR2, hepatitis A virus cellular receptor 2 (TIM-3); LGALS9, lectin galactoside-binding soluble 9 (Galectin-9). Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1682724/abstract'>41112257</a> https://www.bosterbio.com/catalog/product/view/id/1300012026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300022026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300032026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300042026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300052026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300062026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300072026-03-16T05:08:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04474-1-senp3-primary-antibodies-wb-testing-1.jpgAnti-SENP3 AntibodyWestern blot analysis of SENP3 using anti-SENP3 antibody (A04474-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Y79 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP3 antigen affinity purified polyclonal antibody (A04474-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SENP3 at approximately 70 kDa. The expected band size for SENP3 is at 65 kDa. https://www.bosterbio.com/catalog/product/view/id/1300082026-03-13T05:05:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300092026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300102026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300112026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300122026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300132026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300142026-04-03T05:00:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02415-recql-primary-antibodies-wb-testing-1.jpgAnti-RECQL Antibody Picoband®Western blot analysis of RECQL using anti-RECQL antibody (A02415). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RECQL antigen affinity purified polyclonal antibody (A02415) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RECQL at approximately 70-75 kDa. The expected band size for RECQL is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02415-recql-primary-antibodies-ihc-testing-1.jpgAnti-RECQL Antibody Picoband®IHC analysis of RECQL using anti-RECQL antibody (A02415). RECQL was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RECQL Antibody (A02415) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02415-recql-primary-antibodies-ihc-testing-2.jpgAnti-RECQL Antibody Picoband®IHC analysis of RECQL using anti-RECQL antibody (A02415). RECQL was detected in a paraffin-embedded section of human lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RECQL Antibody (A02415) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02415-recql-primary-antibodies-ihc-testing-3.jpgAnti-RECQL Antibody Picoband®IHC analysis of RECQL using anti-RECQL antibody (A02415). RECQL was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RECQL Antibody (A02415) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02415-recql-primary-antibodies-if-testing-1.jpgAnti-RECQL Antibody Picoband®IF analysis of RECQL using anti-RECQL antibody (A02415) and anti-Alpha Tubulin antibody (M03989-3). RECQL was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RECQL Antibody (A02415) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02415-recql-primary-antibodies-fcm-testing-1.jpgAnti-RECQL Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-RECQL antibody (A02415). Overlay histogram showing HEL cells stained with A02415 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RECQL Antibody (A02415, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300152026-04-03T05:00:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02371-1-recql5-primary-antibodies-wb-testing-1.jpgAnti-RECQL5 Antibody Picoband®Western blot analysis of RECQL5 using anti-RECQL5 antibody (A02371-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RECQL5 antigen affinity purified polyclonal antibody (A02371-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RECQL5 at approximately 130 kDa. The expected band size for RECQL5 is at 109 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02371-1-recql5-primary-antibodies-ihc-testing-1.jpgAnti-RECQL5 Antibody Picoband®IHC analysis of RECQL5 using anti-RECQL5 antibody (A02371-1). RECQL5 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RECQL5 Antibody (A02371-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02371-1-recql5-primary-antibodies-ihc-testing-2.jpgAnti-RECQL5 Antibody Picoband®IHC analysis of RECQL5 using anti-RECQL5 antibody (A02371-1). RECQL5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RECQL5 Antibody (A02371-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1300162026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300172026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300182026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300192026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300202026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300212026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300222026-03-16T05:08:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300232026-03-24T05:36:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11880-1-pnma2-primary-antibodies-wb-testing-1.jpgAnti-PNMA2 AntibodyWestern blot analysis of PNMA2 using anti-PNMA2 antibody (A11880-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNMA2 antigen affinity purified polyclonal antibody (A11880-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PNMA2 at approximately 40 kDa. The expected band size for PNMA2 is at 42 kDa. https://www.bosterbio.com/catalog/product/view/id/1300242026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300252026-03-13T05:05:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300262026-03-17T05:16:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01412-3-ncor2-primary-antibodies-wb-testing-1.jpgAnti-NCOR2 Antibody Picoband®Western blot analysis of NCOR2 using anti-NCOR2 antibody (A01412-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOR2 antigen affinity purified polyclonal antibody (A01412-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NCOR2 at approximately 274 kDa. The expected band size for NCOR2 is at 274 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01412-3-ncor2-primary-antibodies-ihc-testing-1.jpgAnti-NCOR2 Antibody Picoband®IHC analysis of NCOR2 using anti-NCOR2 antibody (A01412-3). NCOR2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCOR2 Antibody (A01412-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01412-3-ncor2-primary-antibodies-ihc-testing-2.jpgAnti-NCOR2 Antibody Picoband®IHC analysis of NCOR2 using anti-NCOR2 antibody (A01412-3). NCOR2 was detected in a paraffin-embedded section of human ovarican cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCOR2 Antibody (A01412-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01412-3-ncor2-primary-antibodies-if-testing-1.jpgAnti-NCOR2 Antibody Picoband®IF analysis of NCOR2 using anti-NCOR2 antibody (A01412-3) and anti-Tubulin Alpha antibody (M03989-3). NCOR2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NCOR2 Antibody (A01412-3) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01412-3-ncor2-primary-antibodies-fcm-testing-1.jpgAnti-NCOR2 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-NCOR2 antibody (A01412-3). Overlay histogram showing A549 cells stained with A01412-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCOR2 Antibody (A01412-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300272026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300282026-03-13T05:05:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300292026-03-17T05:16:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04556-1-dyrk1b-primary-antibodies-wb-testing-1.jpgAnti-DYRK1B Antibody Picoband®Western blot analysis of DYRK1B using anti-DYRK1B antibody (A04556-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DYRK1B antigen affinity purified polyclonal antibody (A04556-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DYRK1B at approximately 80 kDa. The expected band size for DYRK1B is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04556-1-dyrk1b-primary-antibodies-fcm-testing-1.jpgAnti-DYRK1B Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-DYRK1B antibody (A04556-1). Overlay histogram showing 293T cells stained with A04556-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DYRK1B Antibody (A04556-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300302026-03-24T05:36:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07707-1-glrx5-primary-antibodies-wb-testing-1.jpgAnti-GLRX5 AntibodyWestern blot analysis of GLRX5 using anti-GLRX5 antibody (A07707-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat herat tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse herat tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLRX5 antigen affinity purified polyclonal antibody (A07707-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GLRX5 at approximately 15 kDa. The expected band size for GLRX5 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07707-1-glrx5-primary-antibodies-ihc-testing-1.jpgAnti-GLRX5 AntibodyIHC analysis of GLRX5 using anti-GLRX5 antibody (A07707-1). GLRX5 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-GLRX5 Antibody (A07707-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07707-1-glrx5-primary-antibodies-ihc-testing-2.jpgAnti-GLRX5 AntibodyIHC analysis of GLRX5 using anti-GLRX5 antibody (A07707-1). GLRX5 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-GLRX5 Antibody (A07707-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07707-1-glrx5-primary-antibodies-ihc-testing-3.jpgAnti-GLRX5 AntibodyIHC analysis of GLRX5 using anti-GLRX5 antibody (A07707-1). GLRX5 was detected in a paraffin-embedded section of human esophageal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-GLRX5 Antibody (A07707-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07707-1-glrx5-primary-antibodies-ihc-testing-4.jpgAnti-GLRX5 AntibodyIHC analysis of GLRX5 using anti-GLRX5 antibody (A07707-1). GLRX5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-GLRX5 Antibody (A07707-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07707-1-glrx5-primary-antibodies-ihc-testing-5.jpgAnti-GLRX5 AntibodyIHC analysis of GLRX5 using anti-GLRX5 antibody (A07707-1). GLRX5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-GLRX5 Antibody (A07707-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07707-1-glrx5-primary-antibodies-ihc-testing-6.jpgAnti-GLRX5 AntibodyIHC analysis of GLRX5 using anti-GLRX5 antibody (A07707-1). GLRX5 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-GLRX5 Antibody (A07707-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07707-1-glrx5-primary-antibodies-ihc-testing-7.jpgAnti-GLRX5 AntibodyIHC analysis of GLRX5 using anti-GLRX5 antibody (A07707-1). GLRX5 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-GLRX5 Antibody (A07707-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1300312026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300322026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300332026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300342026-03-17T05:16:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07225-1-ryk-primary-antibodies-wb-testing-1.jpgAnti-RYK Antibody Picoband®Western blot analysis of RYK using anti-RYK antibody (A07225-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat ovary tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse ovary tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RYK antigen affinity purified polyclonal antibody (A07225-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RYK at approximately 75 kDa. The expected band size for RYK is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07225-1-ryk-primary-antibodies-fcm-testing-1.jpgAnti-RYK Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-RYK antibody (A07225-1). Overlay histogram showing MCF-7 cells stained with A07225-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RYK Antibody (A07225-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300352026-03-17T05:16:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17011-1-pnliprp3-primary-antibodies-wb-testing-1.jpgAnti-PNLIPRP3 Antibody Picoband®Western blot analysis of PNLIPRP3 using anti-PNLIPRP3 antibody (A17011-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HUH-7 whole cell lysates, Lane 3: human HCCT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNLIPRP3 antigen affinity purified polyclonal antibody (A17011-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PNLIPRP3 at approximately 70 kDa. The expected band size for PNLIPRP3 is at 52 kDa. https://www.bosterbio.com/catalog/product/view/id/1300362026-03-17T05:16:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05396-2-ppp1r8-primary-antibodies-wb-testing-1.jpgAnti-PPP1R8 Antibody Picoband®Western blot analysis of PPP1R8 using anti-PPP1R8 antibody (A05396-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R8 antigen affinity purified polyclonal antibody (A05396-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPP1R8 at approximately 39 kDa. The expected band size for PPP1R8 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05396-2-ppp1r8-primary-antibodies-ihc-testing-1.jpgAnti-PPP1R8 Antibody Picoband®IHC analysis of PPP1R8 using anti-PPP1R8 antibody (A05396-2). PPP1R8 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP1R8 Antibody (A05396-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05396-2-ppp1r8-primary-antibodies-ihc-testing-2.jpgAnti-PPP1R8 Antibody Picoband®IHC analysis of PPP1R8 using anti-PPP1R8 antibody (A05396-2). PPP1R8 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP1R8 Antibody (A05396-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05396-2-ppp1r8-primary-antibodies-if-testing-1.jpgAnti-PPP1R8 Antibody Picoband®IF analysis of PPP1R8 using anti-PPP1R8 antibody (A05396-2). PPP1R8 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPP1R8 Antibody (A05396-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05396-2-ppp1r8-primary-antibodies-if-testing-2.jpgAnti-PPP1R8 Antibody Picoband®IF analysis of PPP1R8 using anti-PPP1R8 antibody (A05396-2). PPP1R8 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PPP1R8 Antibody (A05396-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05396-2-ppp1r8-primary-antibodies-fcm-testing-1.jpgAnti-PPP1R8 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-PPP1R8 antibody (A05396-2). Overlay histogram showing HepG2 cells stained with A05396-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R8 Antibody (A05396-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300372026-03-17T05:16:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32228-iqcm-primary-antibodies-wb-testing-1.jpgAnti-IQCM Antibody Picoband®Western blot analysis of IQCM using anti-IQCM antibody (A32228). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IQCM antigen affinity purified polyclonal antibody (A32228) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IQCM at approximately 60 kDa. The expected band size for IQCM is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32228-iqcm-primary-antibodies-ihc-testing-1.jpgAnti-IQCM Antibody Picoband®IHC analysis of IQCM using anti-IQCM antibody (A32228). IQCM was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IQCM Antibody (A32228) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32228-iqcm-primary-antibodies-ihc-testing-2.jpgAnti-IQCM Antibody Picoband®IHC analysis of IQCM using anti-IQCM antibody (A32228). IQCM was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IQCM Antibody (A32228) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1300382026-03-17T05:16:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03746-1-nrcam-primary-antibodies-wb-testing-1.jpgAnti-NRCAM Antibody Picoband®Western blot analysis of NRCAM using anti-NRCAM antibody (A03746-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRCAM antigen affinity purified polyclonal antibody (A03746-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NRCAM at approximately 136 kDa. The expected band size for NRCAM is at 144 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03746-1-nrcam-primary-antibodies-fcm-testing-1.jpgAnti-NRCAM Antibody Picoband®Flow Cytometry analysis of Neuro2a cells using anti-NRCAM antibody (A03746-1). Overlay histogram showing Neuro2a cells stained with A03746-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NRCAM Antibody (A03746-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300392026-03-17T05:16:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08626-2-syf2-primary-antibodies-wb-testing-1.jpgAnti-SYF2 Antibody Picoband®Western blot analysis of SYF2 using anti-SYF2 antibody (A08626-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYF2 antigen affinity purified polyclonal antibody (A08626-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYF2 at approximately 41 kDa. The expected band size for SYF2 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08626-2-syf2-primary-antibodies-fcm-testing-1.jpgAnti-SYF2 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-SYF2 antibody (A08626-2). Overlay histogram showing HEL cells stained with A08626-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYF2 Antibody (A08626-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300402026-03-17T05:16:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07194-gcnt2-primary-antibodies-wb-testing-1.jpgAnti-GCNT2 Antibody Picoband®Western blot analysis of GCNT2 using anti-GCNT2 antibody (A07194). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCNT2 antigen affinity purified polyclonal antibody (A07194) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GCNT2 at approximately 46 kDa. The expected band size for GCNT2 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07194-gcnt2-primary-antibodies-ihc-testing-3.jpgAnti-GCNT2 Antibody Picoband®IHC analysis of GCNT2 using anti-GCNT2 antibody (A07194). GCNT2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GCNT2 Antibody (A07194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07194-gcnt2-primary-antibodies-ihc-testing-2.jpgAnti-GCNT2 Antibody Picoband®IHC analysis of GCNT2 using anti-GCNT2 antibody (A07194). GCNT2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GCNT2 Antibody (A07194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07194-gcnt2-primary-antibodies-ihc-testing-1.jpgAnti-GCNT2 Antibody Picoband®IHC analysis of GCNT2 using anti-GCNT2 antibody (A07194). GCNT2 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GCNT2 Antibody (A07194) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07194-gcnt2-primary-antibodies-if-testing-1.jpgAnti-GCNT2 Antibody Picoband®IF analysis of GCNT2 using anti-GCNT2 antibody (A07194) and anti-Beta Tubulin antibody (M01857-3). GCNT2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GCNT2 Antibody (A07194) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07194-gcnt2-primary-antibodies-if-testing-2.jpgAnti-GCNT2 Antibody Picoband®IF analysis of GCNT2 using anti-GCNT2 antibody (A07194). GCNT2was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GCNT2 Antibody (A07194) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07194-gcnt2-primary-antibodies-if-testing-3.jpgAnti-GCNT2 Antibody Picoband®IF analysis of GCNT2 using anti-GCNT2 antibody (A07194). GCNT2was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GCNT2 Antibody (A07194) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07194-gcnt2-primary-antibodies-fcm-testing-1.jpgAnti-GCNT2 Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-GCNT2 antibody (A07194). Overlay histogram showing Caco-2 cells stained with A07194 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GCNT2 Antibody (A07194, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07194-gcnt2-primary-antibodies-fcm-testing-2.jpgAnti-GCNT2 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-GCNT2 antibody (A07194). Overlay histogram showing HepG2 cells stained with A07194 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GCNT2 Antibody (A07194, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300412026-03-17T05:16:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11536-1-eurl-primary-antibodies-wb-testing-1.jpgAnti-EURL Antibody Picoband®Western blot analysis of EURL using anti-EURL antibody (A11536-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NEuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EURL antigen affinity purified polyclonal antibody (A11536-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EURL at approximately 34 kDa. The expected band size for EURL is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11536-1-eurl-primary-antibodies-fcm-testing-1.jpgAnti-EURL Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-EURL antibody (A11536-1). Overlay histogram showing Caco-2 cells stained with A11536-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EURL Antibody (A11536-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300422026-03-17T05:16:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07086-2-mid2-primary-antibodies-wb-testing-1.jpgAnti-MID2 Antibody Picoband®Western blot analysis of MID2 using anti-MID2 antibody (A07086-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MID2 antigen affinity purified polyclonal antibody (A07086-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MID2 at approximately 83 kDa. The expected band size for MID2 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07086-2-mid2-primary-antibodies-fcm-testing-1.jpgAnti-MID2 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-MID2 antibody (A07086-2). Overlay histogram showing MCF-7 cells stained with A07086-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MID2 Antibody (A07086-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300432026-03-17T05:16:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03830-2-gabbr2-primary-antibodies-wb-testing-1.jpgAnti-GABBR2 Antibody Picoband®Western blot analysis of GABBR2 using anti-GABBR2 antibody (A03830-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABBR2 antigen affinity purified polyclonal antibody (A03830-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GABBR2 at approximately 106 kDa. The expected band size for GABBR2 is at 106 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03830-2-gabbr2-primary-antibodies-ihc-testing-1.jpgAnti-GABBR2 Antibody Picoband®IHC analysis of GABBR2 using anti-GABBR2 antibody (A03830-2). GABBR2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GABBR2 Antibody (A03830-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1300442026-03-17T05:16:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05980-3-hapln1-primary-antibodies-wb-testing-1.jpgAnti-HAPLN1 Antibody Picoband®Western blot analysis of HAPLN1 using anti-HAPLN1 antibody (A05980-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAPLN1 antigen affinity purified polyclonal antibody (A05980-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HAPLN1 at approximately 40,48 kDa. The expected band size for HAPLN1 is at 40,48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05980-3-hapln1-primary-antibodies-ihc-testing-1.jpgAnti-HAPLN1 Antibody Picoband®IHC analysis of HAPLN1 using anti-HAPLN1 antibody (A05980-3). HAPLN1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HAPLN1 Antibody (A05980-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1300452026-03-17T05:16:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15220-ms4a4e-primary-antibodies-wb-testing-1.jpgAnti-MS4A4E Antibody Picoband®Western blot analysis of MS4A4E using anti-MS4A4E antibody (A15220). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: mouse stomach tissue lysates, Lane 5: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MS4A4E antigen affinity purified polyclonal antibody (A15220) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MS4A4E at approximately 19 kDa. The expected band size for MS4A4E is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15220-ms4a4e-primary-antibodies-ihc-testing-1.jpgAnti-MS4A4E Antibody Picoband®IHC analysis of MS4A4E using anti-MS4A4E antibody (A15220). MS4A4E was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MS4A4E Antibody (A15220) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15220-ms4a4e-primary-antibodies-ihc-testing-2.jpgAnti-MS4A4E Antibody Picoband®IHC analysis of MS4A4E using anti-MS4A4E antibody (A15220). MS4A4E was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MS4A4E Antibody (A15220) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1300462026-03-17T05:16:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04595-1-cd42a-gp9-primary-antibodies-wb-testing-1.jpgAnti-CD42a/GP9 Antibody Picoband®Western blot analysis of CD42a/GP9 using anti-CD42a/GP9 antibody (A04595-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD42a/GP9 antigen affinity purified polyclonal antibody (A04595-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD42a/GP9 at approximately 19 kDa. The expected band size for CD42a/GP9 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04595-1-gp9-primary-antibodies-ihc-testing-1.jpgAnti-CD42a/GP9 Antibody Picoband®IHC analysis of CD42a/GP9 using anti-CD42a/GP9 antibody (A04595-1). CD42a/GP9 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD42a/GP9 Antibody (A04595-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1300472026-03-17T05:16:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04595-2-cd42a-gp9-primary-antibodies-wb-testing-1.jpgAnti-CD42a/GP9 Antibody Picoband®Western blot analysis of CD42a/GP9 using anti-CD42a/GP9 antibody (A04595-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD42a/GP9 antigen affinity purified polyclonal antibody (A04595-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD42a/GP9 at approximately 19 kDa. The expected band size for CD42a/GP9 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04595-2-cd42a-gp9-primary-antibodies-ihc-testing-1.jpgAnti-CD42a/GP9 Antibody Picoband®IHC analysis of CD42a/GP9 using anti-CD42a/GP9 antibody (A04595-2). CD42a/GP9 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD42a/GP9 Antibody (A04595-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04595-2-cd42a-gp9-primary-antibodies-wb-testing-2.pngAnti-CD42a/GP9 Antibody Picoband®Western blot analysis of GP9 using anti-GP9 antibody (A04595-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: control group-Mouse hippocampus tissue lysates, Lane 2: model group-Mouse hippocampus tissue lysates, Lane 3: Drug treatment (0.1g/kg) – Mouse hippocampus tissue lysates, , Lane 4: Drug treatment (0.5g/kg) – Mouse hippocampus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GP9 antigen affinity purified polyclonal antibody (A04595-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for GP9 at approximately 19 kDa. The expected band size for GP9 is at 19 kDa. https://www.bosterbio.com/catalog/product/view/id/1300482026-03-17T05:16:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15657-1-ms4a6e-primary-antibodies-wb-testing-1.jpgAnti-MS4A6E Antibody Picoband®Western blot analysis of MS4A6E using anti-MS4A6E antibody (A15657-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MS4A6E antigen affinity purified polyclonal antibody (A15657-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MS4A6E at approximately 18 kDa. The expected band size for MS4A6E is at 16 kDa. https://www.bosterbio.com/catalog/product/view/id/1300492026-03-17T05:16:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11084-1-kcna6-primary-antibodies-wb-testing-1.jpgAnti-KCNA6 Antibody Picoband®Western blot analysis of KCNA6 using anti-KCNA6 antibody (A11084-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA6 antigen affinity purified polyclonal antibody (A11084-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KCNA6 at approximately 59 kDa. The expected band size for KCNA6 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11084-1-kcna6-primary-antibodies-ihc-testing-1.jpgAnti-KCNA6 Antibody Picoband®IHC analysis of KCNA6 using anti-KCNA6 antibody (A11084-1). KCNA6 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KCNA6 Antibody (A11084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11084-1-kcna6-primary-antibodies-ihc-testing-2.jpgAnti-KCNA6 Antibody Picoband®IHC analysis of KCNA6 using anti-KCNA6 antibody (A11084-1). KCNA6 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KCNA6 Antibody (A11084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11084-1-kcna6-primary-antibodies-ihc-testing-3.jpgAnti-KCNA6 Antibody Picoband®IHC analysis of KCNA6 using anti-KCNA6 antibody (A11084-1). KCNA6 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KCNA6 Antibody (A11084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11084-1-kcna6-primary-antibodies-ihc-testing-4.jpgAnti-KCNA6 Antibody Picoband®IHC analysis of KCNA6 using anti-KCNA6 antibody (A11084-1). KCNA6 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KCNA6 Antibody (A11084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11084-1-kcna6-primary-antibodies-ihc-testing-5.jpgAnti-KCNA6 Antibody Picoband®IHC analysis of KCNA6 using anti-KCNA6 antibody (A11084-1). KCNA6 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KCNA6 Antibody (A11084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11084-1-kcna6-primary-antibodies-ihc-testing-6.jpgAnti-KCNA6 Antibody Picoband®IHC analysis of KCNA6 using anti-KCNA6 antibody (A11084-1). KCNA6 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KCNA6 Antibody (A11084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11084-1-kcna6-primary-antibodies-ihc-testing-7.jpgAnti-KCNA6 Antibody Picoband®IHC analysis of KCNA6 using anti-KCNA6 antibody (A11084-1). KCNA6 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KCNA6 Antibody (A11084-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11084-1-kcna6-primary-antibodies-fcm-testing-1.jpgAnti-KCNA6 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-KCNA6 antibody (A11084-1). Overlay histogram showing HEL cells stained with A11084-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNA6 Antibody (A11084-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300502026-03-17T05:16:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04737-3-chp1-primary-antibodies-wb-testing-1.jpgAnti-CHP1 Antibody Picoband®Western blot analysis of CHP1 using anti-CHP1 antibody (A04737-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT-4 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHP1 antigen affinity purified polyclonal antibody (A04737-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CHP1 at approximately 34 kDa. The expected band size for CHP1 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04737-3-chp1-primary-antibodies-if-testing-1.jpgAnti-CHP1 Antibody Picoband®IF analysis of CHP1 using anti-CHP1 antibody (A04737-3) and anti-Beta Tubulin antibody (M01857-3). CHP1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CHP1 Antibody (A04737-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04737-3-chp1-primary-antibodies-fcm-testing-1.jpgAnti-CHP1 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-CHP1 antibody (A04737-3). Overlay histogram showing K562 cells stained with A04737-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHP1 Antibody (A04737-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300512026-03-17T05:16:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10089-1-herc4-primary-antibodies-wb-testing-1.jpgAnti-HERC4 Antibody Picoband®Western blot analysis of HERC4 using anti-HERC4 antibody (A10089-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HERC4 antigen affinity purified polyclonal antibody (A10089-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HERC4 at approximately 107 kDa. The expected band size for HERC4 is at 119 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10089-1-herc4-primary-antibodies-ihc-testing-1.jpgAnti-HERC4 Antibody Picoband®IHC analysis of HERC4 using anti-HERC4 antibody (A10089-1). HERC4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HERC4 Antibody (A10089-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10089-1-herc4-primary-antibodies-ihc-testing-2.jpgAnti-HERC4 Antibody Picoband®IHC analysis of HERC4 using anti-HERC4 antibody (A10089-1). HERC4 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HERC4 Antibody (A10089-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10089-1-herc4-primary-antibodies-ihc-testing-3.jpgAnti-HERC4 Antibody Picoband®IHC analysis of HERC4 using anti-HERC4 antibody (A10089-1). HERC4 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HERC4 Antibody (A10089-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10089-1-herc4-primary-antibodies-fcm-testing-1.jpgAnti-HERC4 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-HERC4 antibody (A10089-1). Overlay histogram showing 293T cells stained with A10089-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HERC4 Antibody (A10089-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300522026-03-17T05:16:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04230-2-ghrhr-primary-antibodies-wb-testing-1.jpgAnti-GHRHR Antibody Picoband®Western blot analysis of GHRHR using anti-GHRHR antibody (A04230-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GHRHR antigen affinity purified polyclonal antibody (A04230-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GHRHR at approximately 47 kDa. The expected band size for GHRHR is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04230-2-ghrhr-primary-antibodies-ihc-testing-1.jpgAnti-GHRHR Antibody Picoband®IHC analysis of GHRHR using anti-GHRHR antibody (A04230-2). GHRHR was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GHRHR Antibody (A04230-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04230-2-ghrhr-primary-antibodies-ihc-testing-2.jpgAnti-GHRHR Antibody Picoband®IHC analysis of GHRHR using anti-GHRHR antibody (A04230-2). GHRHR was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GHRHR Antibody (A04230-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04230-2-ghrhr-primary-antibodies-ihc-testing-3.jpgAnti-GHRHR Antibody Picoband®IHC analysis of GHRHR using anti-GHRHR antibody (A04230-2). GHRHR was detected in a paraffin-embedded section of mouse adrenal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GHRHR Antibody (A04230-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04230-2-ghrhr-primary-antibodies-ihc-testing-4.jpgAnti-GHRHR Antibody Picoband®IHC analysis of GHRHR using anti-GHRHR antibody (A04230-2). GHRHR was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GHRHR Antibody (A04230-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04230-2-ghrhr-primary-antibodies-ihc-testing-5.jpgAnti-GHRHR Antibody Picoband®IHC analysis of GHRHR using anti-GHRHR antibody (A04230-2). GHRHR was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GHRHR Antibody (A04230-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04230-2-ghrhr-primary-antibodies-fcm-testing-1.jpgAnti-GHRHR Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-GHRHR antibody (A04230-2). Overlay histogram showing 293T cells stained with A04230-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GHRHR Antibody (A04230-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300532026-03-17T05:16:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07147-1-nup85-primary-antibodies-wb-testing-1.jpgAnti-NUP85 Antibody Picoband®Western blot analysis of NUP85 using anti-NUP85 antibody (A07147-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP85 antigen affinity purified polyclonal antibody (A07147-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NUP85 at approximately 60 kDa. The expected band size for NUP85 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07147-1-nup85-primary-antibodies-if-testing-1.jpgAnti-NUP85 Antibody Picoband®IF analysis of NUP85 using anti-NUP85 antibody (A07147-1) and anti-Beta Tubulin antibody (M01857-3). NUP85 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUP85 Antibody (A07147-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07147-1-nup85-primary-antibodies-fcm-testing-1.jpgAnti-NUP85 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-NUP85 antibody (A07147-1). Overlay histogram showing Jurkat cells stained with A07147-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUP85 Antibody (A07147-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300542026-03-17T05:16:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04721-2-npy2r-primary-antibodies-wb-testing-1.jpgAnti-NPY2R Antibody Picoband®Western blot analysis of NPY2R using anti-NPY2R antibody (A04721-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPY2R antigen affinity purified polyclonal antibody (A04721-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NPY2R at approximately 43 kDa. The expected band size for NPY2R is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04721-2-npy2r-primary-antibodies-if-testing-1.jpgAnti-NPY2R Antibody Picoband®IF analysis of NPY2R using anti-NPY2R antibody (A04721-2) and anti-Beta Tubulin antibody (M01857-3). NPY2R was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NPY2R Antibody (A04721-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1300552026-03-17T05:16:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07830-2-otud4-primary-antibodies-wb-testing-1.jpgAnti-OTUD4 Antibody Picoband®Western blot analysis of OTUD4 using anti-OTUD4 antibody (A07830-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat H9C2(2-1) whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUD4 antigen affinity purified polyclonal antibody (A07830-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OTUD4 at approximately 150 kDa. The expected band size for OTUD4 is at 124 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07830-2-otud4-primary-antibodies-if-testing-1.jpgAnti-OTUD4 Antibody Picoband®IF analysis of OTUD4 using anti-OTUD4 antibody (A07830-2) and anti-Beta Tubulin antibody (M01857-3). OTUD4 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OTUD4 Antibody (A07830-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07830-2-otud4-primary-antibodies-fcm-testing-1.jpgAnti-OTUD4 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-OTUD4 antibody (A07830-2). Overlay histogram showing K562 cells stained with A07830-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OTUD4 Antibody (A07830-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300562026-03-17T05:16:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00332-2-pam-primary-antibodies-wb-testing-1.jpgAnti-PAM Antibody Picoband®Western blot analysis of PAM using anti-PAM antibody (A00332-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat H9C2(2-1) whole cell lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAM antigen affinity purified polyclonal antibody (A00332-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAM at approximately 120 kDa. The expected band size for PAM is at 108 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00332-2-pam-primary-antibodies-ihc-testing-1.jpgAnti-PAM Antibody Picoband®IHC analysis of PAM using anti-PAM antibody (A00332-2). PAM was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAM Antibody (A00332-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00332-2-pam-primary-antibodies-ihc-testing-2.jpgAnti-PAM Antibody Picoband®IHC analysis of PAM using anti-PAM antibody (A00332-2). PAM was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAM Antibody (A00332-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00332-2-pam-primary-antibodies-ihc-testing-3.jpgAnti-PAM Antibody Picoband®IHC analysis of PAM using anti-PAM antibody (A00332-2). PAM was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAM Antibody (A00332-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00332-2-pam-primary-antibodies-ihc-testing-4.jpgAnti-PAM Antibody Picoband®IHC analysis of PAM using anti-PAM antibody (A00332-2). PAM was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAM Antibody (A00332-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00332-2-pam-primary-antibodies-fcm-testing-1.jpgAnti-PAM Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-PAM antibody (A00332-2). Overlay histogram showing SH-SY5Y cells stained with A00332-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAM Antibody (A00332-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300572026-03-17T05:16:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05429-1-pan3-primary-antibodies-wb-testing-1.jpgAnti-PAN3 Antibody Picoband®Western blot analysis of PAN3 using anti-PAN3 antibody (A05429-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAN3 antigen affinity purified polyclonal antibody (A05429-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAN3 at approximately 96 kDa. The expected band size for PAN3 is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05429-1-pan3-primary-antibodies-ihc-testing-1.jpgAnti-PAN3 Antibody Picoband®IHC analysis of PAN3 using anti-PAN3 antibody (A05429-1). PAN3 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAN3 Antibody (A05429-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05429-1-pan3-primary-antibodies-if-testing-1.jpgAnti-PAN3 Antibody Picoband®IF analysis of PAN3 using anti-PAN3 antibody (A05429-1) and anti-Beta Tubulin antibody (M01857-3). PAN3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PAN3 Antibody (A05429-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05429-1-pan3-primary-antibodies-if-testing-2.jpgAnti-PAN3 Antibody Picoband®IF analysis of PAN3 using anti-PAN3 antibody (A05429-1). PAN3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PAN3 Antibody (A05429-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05429-1-pan3-primary-antibodies-fcm-testing-1.jpgAnti-PAN3 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-PAN3 antibody (A05429-1). Overlay histogram showing THP-1 cells stained with A05429-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAN3 Antibody (A05429-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300582026-03-17T05:16:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04301-1-parg-primary-antibodies-wb-testing-1.jpgAnti-PARG Antibody Picoband®Western blot analysis of PARG using anti-PARG antibody (A04301-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARG antigen affinity purified polyclonal antibody (A04301-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PARG at approximately 130 kDa. The expected band size for PARG is at 111 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04301-1-parg-primary-antibodies-if-testing-1.jpgAnti-PARG Antibody Picoband®IF analysis of PARG using anti-PARG antibody (A04301-1) and anti-Beta Tubulin antibody (M01857-3). PARG was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PARG Antibody (A04301-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04301-1-parg-primary-antibodies-fcm-testing-1.jpgAnti-PARG Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-PARG antibody (A04301-1). Overlay histogram showing K562 cells stained with A04301-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARG Antibody (A04301-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300592026-03-17T05:16:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07910-1-pcyt2-primary-antibodies-wb-testing-1.jpgAnti-PCYT2 Antibody Picoband®Western blot analysis of PCYT2 using anti-PCYT2 antibody (A07910-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCYT2 antigen affinity purified polyclonal antibody (A07910-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCYT2 at approximately 44 kDa. The expected band size for PCYT2 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07910-1-pcyt2-primary-antibodies-if-testing-1.jpgAnti-PCYT2 Antibody Picoband®IF analysis of PCYT2 using anti-PCYT2 antibody (A07910-1) and anti-Beta Tubulin antibody (M01857-3). PCYT2 was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Histone H4 Antibody (A07910-1) and mouse anti-PCYT2 antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07910-1-pcyt2-primary-antibodies-ip-testing-1.jpgAnti-PCYT2 Antibody Picoband®Immunoprecipitating (IP) PCYT2 in K562 whole cell lysate. Western blot analysis of PCYT2 using anti-PCYT2 antibody (A07910-1); Lane 1: K562 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-PCYT2 antibody in K562 whole cell lysate; Lane 3: anti-PCYT2 antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PCYT2 antigen affinity purified polyclonal antibody (A07910-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PCYT2 at approximately 42 kDa. The expected band size for PCYT2 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07910-1-pcyt2-primary-antibodies-fcm-testing-1.jpgAnti-PCYT2 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-PCYT2 antibody (A07910-1). Overlay histogram showing K562 cells stained with A07910-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCYT2 Antibody (A07910-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300602026-03-17T05:16:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10429-1-naa60-primary-antibodies-wb-testing-1.jpgAnti-NAA60 Antibody Picoband®Western blot analysis of NAA60 using anti-NAA60 antibody (A10429-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAA60 antigen affinity purified polyclonal antibody (A10429-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NAA60 at approximately 27 kDa. The expected band size for NAA60 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10429-1-naa60-primary-antibodies-ihc-testing-1.jpgAnti-NAA60 Antibody Picoband®IHC analysis of NAA60 using anti-NAA60 antibody (A10429-1). NAA60 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NAA60 Antibody (A10429-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10429-1-naa60-primary-antibodies-if-testing-1.jpgAnti-NAA60 Antibody Picoband®IF analysis of NAA60 using anti-NAA60 antibody (A10429-1). NAA60 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NAA60 Antibody (A10429-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10429-1-naa60-primary-antibodies-ip-testing-1.jpgAnti-NAA60 Antibody Picoband®Immunoprecipitating NAA60 in MCF-7 whole cell lysate. Western blot analysis of NAA60 using anti-NAA60 antibody (A10429-1). Lane 1: MCF-7 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-NAA60 antibody in MCF-7 whole cell lysate, Lane 3: anti-NAA60 antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NAA60 antigen affinity purified polyclonal antibody (A10429-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NAA60 at approximately 27 kDa. The expected band size for NAA60 is at 27 kDa. https://www.bosterbio.com/catalog/product/view/id/1300612026-03-17T05:16:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07090-1-nup93-primary-antibodies-wb-testing-1_1.jpgAnti-NUP93 Antibody Picoband®Western blot analysis of NUP93 using anti-NUP93 antibody (A07090-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP93 antigen affinity purified polyclonal antibody (A07090-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NUP93 at approximately 93 kDa. The expected band size for NUP93 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07090-1-nup93-primary-antibodies-ihc-testing-1.jpgAnti-NUP93 Antibody Picoband®IHC analysis of NUP93 using anti-NUP93 antibody (A07090-1). NUP93 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUP93 Antibody (A07090-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07090-1-nup93-primary-antibodies-ihc-testing-4.jpgAnti-NUP93 Antibody Picoband®IHC analysis of NUP93 using anti-NUP93 antibody (A07090-1). NUP93 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUP93 Antibody (A07090-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07090-1-nup93-primary-antibodies-ihc-testing-2.jpgAnti-NUP93 Antibody Picoband®IHC analysis of NUP93 using anti-NUP93 antibody (A07090-1). NUP93 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUP93 Antibody (A07090-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07090-1-nup93-primary-antibodies-ihc-testing-3.jpgAnti-NUP93 Antibody Picoband®IHC analysis of NUP93 using anti-NUP93 antibody (A07090-1). NUP93 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUP93 Antibody (A07090-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07090-1-nup93-primary-antibodies-if-testing-1.jpgAnti-NUP93 Antibody Picoband®IF analysis of NUP93 using anti-NUP93 antibody (A07090-1) and anti-Beta Tubulin antibody (M01857-3). NUP93 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUP93 Antibody (A07090-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07090-1-nup93-primary-antibodies-ip-testing-1.jpgAnti-NUP93 Antibody Picoband®Immunoprecipitating NUP93 in A431 whole cell lysate. Western blot analysis of NUP93 using anti-NUP93 antibody (A07090-1). Lane 1: A431 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-NUP93 antibody in A431 whole cell lysate, Lane 3: anti-NUP93 antibody (2μg) + A431 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NUP93 antigen affinity purified polyclonal antibody (A07090-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NUP93 at approximately 93 kDa. The expected band size for NUP93 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07090-1-nup93-primary-antibodies-fcm-testing-1.jpgAnti-NUP93 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-NUP93 antibody (A07090-1). Overlay histogram showing K562 cells stained with A07090-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUP93 Antibody (A07090-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300622026-03-17T05:16:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01130-1-pbrm1-primary-antibodies-wb-testing-1.jpgAnti-PBRM1 Antibody Picoband®Western blot analysis of PBRM1 using anti-PBRM1 antibody (A01130-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PBRM1 antigen affinity purified polyclonal antibody (A01130-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PBRM1 at approximately 240 kDa. The expected band size for PBRM1 is at 193 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01130-1-pbrm1-primary-antibodies-fcm-testing-1.jpgAnti-PBRM1 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-PBRM1 antibody (A01130-1). Overlay histogram showing K562 cells stained with A01130-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PBRM1 Antibody (A01130-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01130-1-pbrm1-primary-antibodies-if-testing-1.jpgAnti-PBRM1 Antibody Picoband®IF analysis of PBRM1 using anti-PBRM1 antibody (A01130-1) and anti-Alpha Tubulin antibody (M03989-3). PBRM1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PBRM1 Antibody (A01130-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1300632026-03-17T05:16:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04507-1-scap-primary-antibodies-wb-testing-1.jpgAnti-SCAP Antibody Picoband®Western blot analysis of SCAP using anti-SCAP antibody (A04507-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCAP antigen affinity purified polyclonal antibody (A04507-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SCAP at approximately 140 kDa. The expected band size for SCAP is at 140 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04507-1-scap-primary-antibodies-fcm-testing-1.jpgAnti-SCAP Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-SCAP antibody (A04507-1). Overlay histogram showing 293T cells stained with A04507-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCAP Antibody (A04507-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04507-1-scap-primary-antibodies-fcm-testing-2.jpgAnti-SCAP Antibody Picoband®Flow Cytometry analysis of HEPA1-6 cells using anti-SCAP antibody (A04507-1). Overlay histogram showing HEPA1-6 cells stained with A04507-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCAP Antibody (A04507-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04507-1-scap-primary-antibodies-fcm-testing-3.jpgAnti-SCAP Antibody Picoband®Flow Cytometry analysis of C6 cells using anti-SCAP antibody (A04507-1). Overlay histogram showing C6 cells stained with A04507-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCAP Antibody (A04507-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300652026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300662026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300672026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300682026-03-13T05:05:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300692026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300702026-04-02T05:01:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14073-1-emc2-primary-antibodies-wb-testing-1.jpgAnti-TTC35/EMC2 Antibody Picoband®Western blot analysis of TTC35/EMC2 using anti-TTC35/EMC2 antibody (A14073-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC35/EMC2 antigen affinity purified polyclonal antibody (A14073-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TTC35/EMC2 at approximately 35 kDa. The expected band size for TTC35/EMC2 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14073-1-emc2-primary-antibodies-ihc-testing-1.jpgAnti-TTC35/EMC2 Antibody Picoband®IHC analysis of TTC35/EMC2 using anti-TTC35/EMC2 antibody (A14073-1). TTC35/EMC2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC35/EMC2 Antibody (A14073-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14073-1-emc2-primary-antibodies-ihc-testing-2.jpgAnti-TTC35/EMC2 Antibody Picoband®IHC analysis of TTC35/EMC2 using anti-TTC35/EMC2 antibody (A14073-1). TTC35/EMC2 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC35/EMC2 Antibody (A14073-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14073-1-emc2-primary-antibodies-fcm-testing-1.jpgAnti-TTC35/EMC2 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-TTC35/EMC2 antibody (A14073-1). Overlay histogram showing Jurkat cells stained with A14073-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTC35/EMC2 Antibody (A14073-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300712026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300722026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300732026-03-17T05:16:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02442-2-ffar2-primary-antibodies-wb-testing-1.jpgAnti-FFAR2 Antibody Picoband®Western blot analysis of FFAR2 using anti-FFAR2 antibody (A02442-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FFAR2 antigen affinity purified polyclonal antibody (A02442-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FFAR2 at approximately 37 kDa. The expected band size for FFAR2 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02442-2-ffar2-primary-antibodies-fcm-testing-1.jpgAnti-FFAR2 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-FFAR2 antibody (A02442-2). Overlay histogram showing THP-1 cells stained with A02442-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-FFAR2 Antibody (A02442-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300742026-03-13T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03199-1-gjb3-primary-antibodies-wb-testing-1.jpgAnti-GJB3 AntibodyWestern blot analysis of GJB3 using anti-GJB3 antibody (A03199-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat ovary tissue lysates, Lane 6: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJB3 antigen affinity purified polyclonal antibody (A03199-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GJB3 at approximately 31 kDa. The expected band size for GJB3 is at 31 kDa. https://www.bosterbio.com/catalog/product/view/id/1300752026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300772026-03-13T05:05:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300782026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300792026-03-17T05:16:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00803-3-tbx5-primary-antibodies-wb-testing-1.jpgAnti-TBX5 Antibody Picoband®Western blot analysis of TBX5 using anti-TBX5 antibody (A00803-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBX5 antigen affinity purified polyclonal antibody (A00803-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TBX5 at approximately 65 kDa. The expected band size for TBX5 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00803-3-tbx5-primary-antibodies-ihc-testing-1.jpgAnti-TBX5 Antibody Picoband®IHC analysis of TBX5 using anti-TBX5 antibody (A00803-3). TBX5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX5 Antibody (A00803-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00803-3-tbx5-primary-antibodies-ihc-testing-2.jpgAnti-TBX5 Antibody Picoband®IHC analysis of TBX5 using anti-TBX5 antibody (A00803-3). TBX5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX5 Antibody (A00803-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00803-3-tbx5-primary-antibodies-ihc-testing-3.jpgAnti-TBX5 Antibody Picoband®IHC analysis of TBX5 using anti-TBX5 antibody (A00803-3). TBX5 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX5 Antibody (A00803-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00803-3-tbx5-primary-antibodies-ihc-testing-4.jpgAnti-TBX5 Antibody Picoband®IHC analysis of TBX5 using anti-TBX5 antibody (A00803-3). TBX5 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX5 Antibody (A00803-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00803-3-tbx5-primary-antibodies-ihc-testing-5.jpgAnti-TBX5 Antibody Picoband®IHC analysis of TBX5 using anti-TBX5 antibody (A00803-3). TBX5 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX5 Antibody (A00803-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00803-3-tbx5-primary-antibodies-if-testing-1.jpgAnti-TBX5 Antibody Picoband®IF analysis of TBX5 using anti-TBX5 antibody (A00803-3). TBX5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TBX5 Antibody (A00803-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00803-3-tbx5-primary-antibodies-fcm-testing-1.jpgAnti-TBX5 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-TBX5 antibody (A00803-3). Overlay histogram showing MCF-7 cells stained with A00803-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TBX5 Antibody (A00803-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300802026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300812026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300822026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300832026-03-13T05:05:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300842026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300852026-03-17T05:16:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02182-2-egln3-primary-antibodies-wb-testing-1.jpgAnti-EGLN3 Antibody Picoband®Western blot analysis of EGLN3 using anti-EGLN3 antibody (A02182-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGLN3 antigen affinity purified polyclonal antibody (A02182-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EGLN3 at approximately 32 kDa. The expected band size for EGLN3 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02182-2-egln3-primary-antibodies-ihc-testing-1.jpgAnti-EGLN3 Antibody Picoband®IHC analysis of EGLN3 using anti-EGLN3 antibody (A02182-2). EGLN3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EGLN3 Antibody (A02182-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1300862026-03-17T05:16:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05883-2-rab31-primary-antibodies-wb-testing-1.jpgAnti-RAB31 Antibody Picoband®Western blot analysis of RAB31 using anti-RAB31 antibody (A05883-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB31 antigen affinity purified polyclonal antibody (A05883-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB31 at approximately 22 kDa. The expected band size for RAB31 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05883-2-rab31-primary-antibodies-if-testing-1.jpgAnti-RAB31 Antibody Picoband®IF analysis of RAB31 using anti-RAB31 antibody (A05883-2). RAB31 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAB31 Antibody (A05883-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05883-2-rab31-primary-antibodies-fcm-testing-1.jpgAnti-RAB31 Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-RAB31 antibody (A05883-2). Overlay histogram showing HeLa cells stained with A05883-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB31 Antibody (A05883-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300872026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300882026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300892026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300902026-03-17T05:16:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01407-2-cpe-primary-antibodies-wb-testing-1.jpgAnti-Carboxypeptidase E/CPE Antibody Picoband®Western blot analysis of CPE using anti-CPE antibody (A01407-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPE antigen affinity purified polyclonal antibody (A01407-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CPE at approximately 53 kDa. The expected band size for CPE is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01407-2-cpe-primary-antibodies-ihc-testing-1.jpgAnti-Carboxypeptidase E/CPE Antibody Picoband®IHC analysis of CPE using anti-CPE antibody (A01407-2). CPE was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CPE Antibody (A01407-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01407-2-cpe-primary-antibodies-ihc-testing-2.jpgAnti-Carboxypeptidase E/CPE Antibody Picoband®IHC analysis of CPE using anti-CPE antibody (A01407-2). CPE was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CPE Antibody (A01407-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01407-2-cpe-primary-antibodies-ip-testing-1.jpgAnti-Carboxypeptidase E/CPE Antibody Picoband®Immunoprecipitating (IP) CPE in MCF-7 whole cell lysate. Western blot analysis of CPE using anti-CPE antibody (A01407-2); Lane 1: MCF-7 whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-CPE antibody in MCF-7 whole cell lysate; Lane 3: anti-CPE antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CPE antigen affinity purified polyclonal antibody (A01407-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Light Chian). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for CPE at approximately 53 kDa. The expected band size for CPE is at 53 kDa. https://www.bosterbio.com/catalog/product/view/id/1300912026-03-16T05:08:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300922026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300932026-03-16T05:08:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05101-1-crat-primary-antibodies-wb-testing-1.jpgAnti-CRAT AntibodyWestern blot analysis of CRAT using anti-CRAT antibody (A05101-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRAT antigen affinity purified polyclonal antibody (A05101-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CRAT at approximately 62 kDa. The expected band size for CRAT is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05101-1-crat-primary-antibodies-ihc-testing-1.jpgAnti-CRAT AntibodyIHC analysis of CRAT using anti-CRAT antibody (A05101-1). CRAT was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-CRAT Antibody (A05101-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05101-1-crat-primary-antibodies-ihc-testing-2.jpgAnti-CRAT AntibodyIHC analysis of CRAT using anti-CRAT antibody (A05101-1). CRAT was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-CRAT Antibody (A05101-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1300942026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300952026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300962026-03-17T05:16:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01448-1-ihh-primary-antibodies-ihc-testing-1.jpgAnti-IHH Antibody Picoband®IHC analysis of IHH using anti-IHH antibody (A01448-1). IHH was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-IHH Antibody (A01448-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01448-1-ihh-primary-antibodies-wb-testing-1.jpgAnti-IHH Antibody Picoband®Western blot analysis of IHH using anti-IHH antibody (A01448-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IHH antigen affinity purified polyclonal antibody (A01448-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IHH at approximately 45 kDa. The expected band size for IHH is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01448-1-ihh-primary-antibodies-ihc-testing-1_1.jpgAnti-IHH Antibody Picoband®IHC analysis of IHH using anti-IHH antibody (A01448-1). IHH was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IHH Antibody (A01448-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01448-1-ihh-primary-antibodies-if-testing-1.jpgAnti-IHH Antibody Picoband®IF analysis of IHH using anti-IHH antibody (A01448-1). IHH was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-IHH Antibody (A01448-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01448-1-ihh-primary-antibodies-fcm-testing-1.jpgAnti-IHH Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-IHH antibody (A01448-1). Overlay histogram showing RT4 cells stained with A01448-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IHH Antibody (A01448-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1300972026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300982026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1300992026-03-17T05:16:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03763-1-pdlim5-primary-antibodies-wb-testing-1.jpgAnti-PDLIM5 Antibody Picoband®Western blot analysis of PDLIM5 using anti-PDLIM5 antibody (A03763-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDLIM5 antigen affinity purified polyclonal antibody (A03763-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDLIM5 at approximately 64 kDa. The expected band size for PDLIM5 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03763-1-pdlim5-primary-antibodies-ihc-testing-1.jpgAnti-PDLIM5 Antibody Picoband®IHC analysis of PDLIM5 using anti-PDLIM5 antibody (A03763-1). PDLIM5 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDLIM5 Antibody (A03763-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03763-1-pdlim5-primary-antibodies-if-testing-1.jpgAnti-PDLIM5 Antibody Picoband®IF analysis of PDLIM5 using anti-PDLIM5 antibody (A03763-1). PDLIM5 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PDLIM5 Antibody (A03763-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1301002026-03-16T05:08:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04779-2-scg2-primary-antibodies-wb-testing-1.jpgAnti-Secretogranin II/SCG2 AntibodyWestern blot analysis of Secretogranin II/SCG2 using anti-Secretogranin II/SCG2 antibody (A04779-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Secretogranin II/SCG2 antigen affinity purified polyclonal antibody (A04779-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Secretogranin II/SCG2 at approximately 71 kDa. The expected band size for Secretogranin II/SCG2 is at 71 kDa. https://www.bosterbio.com/catalog/product/view/id/1301012026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301022026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301032026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301042026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301052026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301062026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301072026-03-17T05:16:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03089-1-dclk1-primary-antibodies-wb-testing-1.jpgAnti-DCLK1 Antibody Picoband®Western blot analysis of DCLK1 using anti-DCLK1 antibody (A03089-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCLK1 antigen affinity purified polyclonal antibody (A03089-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCLK1 at approximately 85 kDa. The expected band size for DCLK1 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03089-1-dclk1-primary-antibodies-ihc-testing-1.jpgAnti-DCLK1 Antibody Picoband®IHC analysis of DCLK1 using anti-DCLK1 antibody (A03089-1). DCLK1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DCLK1 Antibody (A03089-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03089-1-dclk1-primary-antibodies-ihc-testing-2.jpgAnti-DCLK1 Antibody Picoband®IHC analysis of DCLK1 using anti-DCLK1 antibody (A03089-1). DCLK1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DCLK1 Antibody (A03089-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03089-1-dclk1-primary-antibodies-fcm-testing-1.jpgAnti-DCLK1 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-DCLK1 antibody (A03089-1). Overlay histogram showing SH-SY5Y cells stained with A03089-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DCLK1 Antibody (A03089-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1301082026-03-16T05:08:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07077-3-dgkh-primary-antibodies-wb-testing-1.jpgAnti-DGKH Antibody Picoband®Western blot analysis of DGKH using anti-DGKH antibody (A07077-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DGKH antigen affinity purified polyclonal antibody (A07077-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DGKH at approximately 135 kDa. The expected band size for DGKH is at 135 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07077-3-dgkh-primary-antibodies-ihc-testing-1_1.jpgAnti-DGKH Antibody Picoband®IHC analysis of DGKH using anti-DGKH antibody (A07077-3). DGKH was detected in a paraffin-embedded section of human kidney cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DGKH Antibody (A07077-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1301092026-04-02T05:01:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06640-1-ero1a-primary-antibodies-wb-testing-1.jpgAnti-ERO1L/ERO1A Antibody Picoband®Western blot analysis of ERO1L/ERO1A using anti-ERO1L/ERO1A antibody (A06640-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat SHZ-88 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERO1L/ERO1A antigen affinity purified polyclonal antibody (A06640-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERO1L/ERO1A at approximately 54 kDa. The expected band size for ERO1L/ERO1A is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06640-1-ero1a-primary-antibodies-ihc-testing-1.jpgAnti-ERO1L/ERO1A Antibody Picoband®IHC analysis of ERO1L/ERO1A using anti-ERO1L/ERO1A antibody (A06640-1). ERO1L/ERO1A was detected in a paraffin-embedded section of human esophagus cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ERO1L/ERO1A Antibody (A06640-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1301102026-04-03T05:00:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05229-1-fbxo5-primary-antibodies-wb-testing-1.jpgAnti-FBXO5 Antibody Picoband®Western blot analysis of FBXO5 using anti-FBXO5 antibody (A05229-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXO5 antigen affinity purified polyclonal antibody (A05229-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXO5 at approximately 65 kDa. The expected band size for FBXO5 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05229-1-fbxo5-primary-antibodies-fcm-testing-1.jpgAnti-FBXO5 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-FBXO5 antibody (A05229-1). Overlay histogram showing K562 cells stained with A05229-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FBXO5 Antibody (A05229-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1301112026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301122026-03-16T05:08:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31778-1-gucy1a3-primary-antibodies-wb-testing-1.jpgAnti-GUCY1A3/GUCY1A1 AntibodyWestern blot analysis of GUCY1A3 using anti-GUCY1A3 antibody (A31778-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GUCY1A3 antigen affinity purified polyclonal antibody (A31778-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GUCY1A3 at approximately 77 kDa. The expected band size for GUCY1A3 is at 77 kDa. https://www.bosterbio.com/catalog/product/view/id/1301132026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301142026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301152026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301162026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301172026-03-17T05:16:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06930-pdss2-primary-antibodies-wb-testing-1.jpgAnti-PDSS2 Antibody Picoband®Western blot analysis of PDSS2 using anti-PDSS2 antibody (A06930). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDSS2 antigen affinity purified polyclonal antibody (A06930) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDSS2 at approximately 40 kDa. The expected band size for PDSS2 is at 44 kDa. https://www.bosterbio.com/catalog/product/view/id/1301182026-03-13T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02638-3-pip5k3-primary-antibodies-wb-testing-1.jpgAnti-PIP5K3/PIKFYVE AntibodyWestern blot analysis of PIP5K3 using anti-PIP5K3 antibody (A02638-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIP5K3 antigen affinity purified polyclonal antibody (A02638-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIP5K3 at approximately 237 kDa. The expected band size for PIP5K3 is at 237 kDa. https://www.bosterbio.com/catalog/product/view/id/1301192026-03-17T05:16:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03159-2-piwil1-primary-antibodies-wb-testing-1.jpgAnti-PIWIL1 Antibody Picoband®Western blot analysis of PIWIL1 using anti-PIWIL1 antibody (A03159-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIWIL1 antigen affinity purified polyclonal antibody (A03159-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIWIL1 at approximately 99 kDa. The expected band size for PIWIL1 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03159-2-piwil1-primary-antibodies-fcm-testing-1.jpgAnti-PIWIL1 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-PIWIL1 antibody (A03159-2). Overlay histogram showing RT4 cells stained with A03159-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIWIL1 Antibody (A03159-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1301202026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301212026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301222026-03-16T05:08:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05103-1-prkg2-primary-antibodies-wb-testing-1.jpgAnti-PRKG2 AntibodyWestern blot analysis of PRKG2 using anti-PRKG2 antibody (A05103-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKG2 antigen affinity purified polyclonal antibody (A05103-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRKG2 at approximately 87 kDa. The expected band size for PRKG2 is at 87 kDa. https://www.bosterbio.com/catalog/product/view/id/1301232026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301242026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301252026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301262026-03-16T05:08:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301272026-03-16T05:08:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301282026-03-16T05:08:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301292026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301302026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301312026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301322026-03-16T05:08:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301332026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09570-3-ubash3b-primary-antibodies-wb-testing-1.jpgAnti-STS 1/UBASH3B AntibodyWestern blot analysis of STS 1/UBASH3B using anti-STS 1/UBASH3B antibody (A09570-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STS 1/UBASH3B antigen affinity purified polyclonal antibody (A09570-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STS 1/UBASH3B at approximately 70 kDa. The expected band size for STS 1/UBASH3B is at 73 kDa. https://www.bosterbio.com/catalog/product/view/id/1301342026-03-13T05:05:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301352026-03-16T05:08:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08383-1-ufl1-primary-antibodies-wb-testing-1.jpgAnti-UFL1 AntibodyWestern blot analysis of UFL1 using anti-UFL1 antibody (A08383-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UFL1 antigen affinity purified polyclonal antibody (A08383-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UFL1 at approximately 100 kDa. The expected band size for UFL1 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08383-1-ufl1-primary-antibodies-ihc-testing-1.jpgAnti-UFL1 AntibodyIHC analysis of UFL1 using anti-UFL1 antibody (A08383-1). UFL1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-UFL1 Antibody (A08383-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1301362026-03-16T05:08:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301372026-03-16T05:08:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301382026-03-16T05:08:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301392026-03-16T05:08:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301402026-03-16T05:08:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301412026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301422026-03-16T05:08:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301432026-03-16T05:08:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301442026-03-16T05:08:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301452026-03-17T05:16:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09719-2-dnai2-primary-antibodies-wb-testing-1.jpgAnti-DNAI2 Antibody Picoband®Western blot analysis of DNAI2 using anti-DNAI2 antibody (A09719-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human BEAS-2B whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat ovary tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAI2 antigen affinity purified polyclonal antibody (A09719-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNAI2 at approximately 69 kDa. The expected band size for DNAI2 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09719-2-dnai2-primary-antibodies-fcm-testing-1.jpgAnti-DNAI2 Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-DNAI2 antibody (A09719-2). Overlay histogram showing HeLa cells stained with A09719-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAI2 Antibody (A09719-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1301462026-04-02T05:01:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07556-3-dpf2-primary-antibodies-wb-testing-1.jpgAnti-DPF2 Antibody Picoband®Western blot analysis of DPF2 using anti-DPF2 antibody (A07556-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Y79 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPF2 antigen affinity purified polyclonal antibody (A07556-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DPF2 at approximately 40 kDa. The expected band size for DPF2 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07556-3-dpf2-primary-antibodies-ihc-testing-1.jpgAnti-DPF2 Antibody Picoband®IHC analysis of DPF2 using anti-DPF2 antibody (A07556-3). DPF2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DPF2 Antibody (A07556-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07556-3-dpf2-primary-antibodies-ihc-testing-2.jpgAnti-DPF2 Antibody Picoband®IHC analysis of DPF2 using anti-DPF2 antibody (A07556-3). DPF2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DPF2 Antibody (A07556-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07556-3-dpf2-primary-antibodies-if-testing-1.jpgAnti-DPF2 Antibody Picoband®IF analysis of DPF2 using anti-DPF2 antibody (A07556-3) and anti-Alpha Tubulin antibody (M03989-3). DPF2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DPF2 Antibody (A07556-3) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1301472026-03-16T05:08:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301482026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301492026-04-02T05:01:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07926-3-exoc7-primary-antibodies-wb-testing-1.jpgAnti-Exo70/EXOC7 Antibody Picoband®Western blot analysis of Exo70/EXOC7 using anti-Exo70/EXOC7 antibody (A07926-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Exo70/EXOC7 antigen affinity purified polyclonal antibody (A07926-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Exo70/EXOC7 at approximately 75 kDa. The expected band size for Exo70/EXOC7 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07926-3-exoc7-primary-antibodies-ihc-testing-1.jpgAnti-Exo70/EXOC7 Antibody Picoband®IHC analysis of Exo70/EXOC7 using anti-Exo70/EXOC7 antibody (A07926-3). Exo70/EXOC7 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Exo70/EXOC7 Antibody (A07926-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07926-3-exoc7-primary-antibodies-fcm-testing-1.jpgAnti-Exo70/EXOC7 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-Exo70/EXOC7 antibody (A07926-3). Overlay histogram showing RT4 cells stained with A07926-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Exo70/EXOC7 Antibody (A07926-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1301502026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301512026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301522026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301532026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301542026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301552026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301562026-03-16T05:08:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32396-1-gucy1b3-primary-antibodies-wb-testing-1.jpgAnti-GUCY1B3/GUCY1B1 AntibodyWestern blot analysis of GUCY1B3 using anti-GUCY1B3 antibody (A32396-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GUCY1B3 antigen affinity purified polyclonal antibody (A32396-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GUCY1B3 at approximately 71 kDa. The expected band size for GUCY1B3 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32396-1-gucy1b3-primary-antibodies-ihc-testing-1.jpgAnti-GUCY1B3/GUCY1B1 AntibodyIHC analysis of GUCY1B3 using anti-GUCY1B3 antibody (A32396-1). GUCY1B3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-GUCY1B3 Antibody (A32396-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32396-1-gucy1b3-primary-antibodies-ihc-testing-2.jpgAnti-GUCY1B3/GUCY1B1 AntibodyIHC analysis of GUCY1B3 using anti-GUCY1B3 antibody (A32396-1). GUCY1B3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-GUCY1B3 Antibody (A32396-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1301572026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301582026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301592026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301602026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301612026-03-16T05:08:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10051-itm2a-primary-antibodies-wb-testing-1.jpgAnti-ITM2A AntibodyWestern blot analysis of ITM2A using anti-ITM2A antibody (A10051). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITM2A antigen affinity purified polyclonal antibody (A10051) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ITM2A at approximately 32 kDa. The expected band size for ITM2A is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10051-itm2a-primary-antibodies-wb-testing-1_1.jpgAnti-ITM2A AntibodyWestern blot analysis of ITM2A using anti-ITM2A antibody (A10051). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITM2A antigen affinity purified polyclonal antibody (A10051) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ITM2A at approximately 32 kDa. The expected band size for ITM2A is at 30 kDa. https://www.bosterbio.com/catalog/product/view/id/1301622026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301632026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301642026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301652026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301662026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301672026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301682026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301692026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301702026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301712026-03-16T05:08:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301722026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301732026-03-17T05:16:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05596-1-ndst1-primary-antibodies-wb-testing-1.jpgAnti-NDST1 AntibodyWestern blot analysis of NDST1 using anti-NDST1 antibody (A05596-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HUVEC whole cell lysates. Lane 2: human A431 whole cell lysates. Lane 3: human CACO-2 whole cell lysates. Lane 4: rat heart tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDST1 antigen affinity purified polyclonal antibody (A05596-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NDST1 at approximately 70 kDa. The expected band size for NDST1 is at 101 kDa. https://www.bosterbio.com/catalog/product/view/id/1301742026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08311-1-nnat-primary-antibodies-ihc-testing-1.jpgAnti-Neuronatin/NNAT AntibodyIHC analysis of Neuronatin/NNAT using anti-Neuronatin/NNAT antibody (A08311-1). Neuronatin/NNAT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Neuronatin/NNAT Antibody (A08311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08311-1-nnat-primary-antibodies-ihc-testing-2.jpgAnti-Neuronatin/NNAT AntibodyIHC analysis of Neuronatin/NNAT using anti-Neuronatin/NNAT antibody (A08311-1). Neuronatin/NNAT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Neuronatin/NNAT Antibody (A08311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08311-1-nnat-primary-antibodies-ihc-testing-3.jpgAnti-Neuronatin/NNAT AntibodyIHC analysis of Neuronatin/NNAT using anti-Neuronatin/NNAT antibody (A08311-1). Neuronatin/NNAT was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Neuronatin/NNAT Antibody (A08311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08311-1-nnat-primary-antibodies-ihc-testing-4.jpgAnti-Neuronatin/NNAT AntibodyIHC analysis of Neuronatin/NNAT using anti-Neuronatin/NNAT antibody (A08311-1). Neuronatin/NNAT was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Neuronatin/NNAT Antibody (A08311-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08311-1-nnat-primary-antibodies-if-testing-1.jpgAnti-Neuronatin/NNAT AntibodyIF analysis of Neuronatin/NNAT using anti-Neuronatin/NNAT antibody (A08311-1). Neuronatin/NNAT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Neuronatin/NNAT Antibody (A08311-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08311-1-nnat-primary-antibodies-if-testing-2.jpgAnti-Neuronatin/NNAT AntibodyIF analysis of Neuronatin/NNAT using anti-Neuronatin/NNAT antibody (A08311-1). Neuronatin/NNAT was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Neuronatin/NNAT Antibody (A08311-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08311-1-nnat-primary-antibodies-fcm-testing-1.jpgAnti-Neuronatin/NNAT AntibodyFlow Cytometry analysis of RT4 cells using anti-Neuronatin/NNAT antibody (A08311-1). Overlay histogram showing RT4 cells stained with A08311-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Neuronatin/NNAT Antibody (A08311-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1301752026-03-17T05:16:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301762026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301772026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301782026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301792026-03-17T05:16:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06329-1-tymp-primary-antibodies-wb-testing-1.jpgAnti-PD-ECGF/TYMP Antibody Picoband®Western blot analysis of PD-ECGF/TYMP using anti-PD-ECGF/TYMP antibody (A06329-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-ECGF/TYMP antigen affinity purified polyclonal antibody (A06329-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PD-ECGF/TYMP at approximately 50 kDa. The expected band size for PD-ECGF/TYMP is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06329-1-tymp-primary-antibodies-ip-testing-1.jpgAnti-PD-ECGF/TYMP Antibody Picoband®Immunoprecipitating PD-ECGF/TYMP in SiHa whole cell lysate. Western blot analysis of PD-ECGF/TYMP using anti-PD-ECGF/TYMP antibody (A06329-1). Lane 1: SiHa whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-PD-ECGF/TYMP antibody in SiHa whole cell lysate, Lane 3: anti-PD-ECGF/TYMP antibody (2μg) + SiHa whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PD-ECGF/TYMP antigen affinity purified polyclonal antibody (A06329-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PD-ECGF/TYMP at approximately 50 kDa. The expected band size for PD-ECGF/TYMP is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06329-1-tymp-primary-antibodies-fcm-testing-1.jpgAnti-PD-ECGF/TYMP Antibody Picoband®Flow Cytometry analysis of A431 cells using anti-PD-ECGF/TYMP antibody (A06329-1). Overlay histogram showing A431 cells stained with A06329-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PD-ECGF/TYMP Antibody (A06329-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06329-1-tymp-primary-antibodies-fcm-testing-2.jpgAnti-PD-ECGF/TYMP Antibody Picoband®Flow Cytometry analysis of SiHa cells using anti-PD-ECGF/TYMP antibody (A06329-1). Overlay histogram showing SiHa cells stained with A06329-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PD-ECGF/TYMP Antibody (A06329-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1301802026-03-17T05:16:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07018-pelo-primary-antibodies-wb-testing-1.jpgAnti-PELO Antibody Picoband®Western blot analysis of PELO using anti-PELO antibody (A07018). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat Kidney tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PELO antigen affinity purified polyclonal antibody (A07018) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PELO at approximately 45 kDa. The expected band size for PELO is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07018-pelo-primary-antibodies-ihc-testing-1.jpgAnti-PELO Antibody Picoband®IHC analysis of PELO using anti-PELO antibody (A07018). PELO was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PELO Antibody (A07018) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07018-pelo-primary-antibodies-ihc-testing-2.jpgAnti-PELO Antibody Picoband®IHC analysis of PELO using anti-PELO antibody (A07018). PELO was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PELO Antibody (A07018) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1301812026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301822026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301832026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301842026-03-13T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02993-2-pitx1-primary-antibodies-wb-testing-1.jpgAnti-PITX1 AntibodyWestern blot analysis of PITX1 using anti-PITX1 antibody (A02993-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITX1 antigen affinity purified polyclonal antibody (A02993-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PITX1 at approximately 40 kDa. The expected band size for PITX1 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02993-2-12931_2025_3222_fig1_html.pngAnti-PITX1 AntibodyUpregulation of PITX1 in hypoxic mice and PASMCs. A , B Immunofluorescence staining: Localization and statistical data of PITX1 in tissues. Scale bar: 25 μm. C Western blot: Representative image and statistical data of PITX1 expression in the lung tissues of mice. D RT‒qPCR: Changes in the transcript level of PITX1 in the lung tissues of mice. E Western blot: Representative image and statistical data of PITX1 expression in the smooth muscle cells of mice. F RT‒qPCR: Changes in the transcript level of PITX1 in the smooth muscle cells of mice. Nor normoxic, Hyp hypoxic. All values are presented as means ± SEMs (* p < 0.05, ** p < 0.01, and *** p < 0.001; n ≥ 3) Index in PubMed under a CC BY license. PMID: <a href='https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03222-9'>40241046</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02993-2-12931_2025_3222_fig2_html.pngAnti-PITX1 AntibodyImpact of PITX1 on pyroptosis in hypoxia-induced PASMCs. A GSEA: The functions of PITX1 were enriched mainly in the inflammasome signaling pathway under hypoxic conditions. B , C Western blot: The interference efficiency of siPITX1 and the overexpression efficiency of the PITX1 plasmid. D , I Western blot: Representative images and statistical data of CASP1, GSDMD-N, IL-1β, and IL-18 levels. E , J YPI/PI staining: YPI is a green fluorescent dye permeable to the membrane of apoptotic cells, and PI staining of necrotic cells with compromised membrane integrity may result in the emission of red fluorescence. Scale bar: 100 μm. F , K DiO staining: Green fluorescence staining of the cell membrane; and the membranes of live cells exhibit green fluorescence. Scale bar: 50 μm. G , H LDH assay: Determination of the number of dead cells by measuring the amount of LDH released from the membrane of damaged cells. Nor normoxic, Hyp hypoxic, NC noncoding nucleotides, siPITX PITX1 siRNA, and p-PITX PITX1 plasmid. All values are presented as means ± SEMs (* p < 0.05, and ** p < 0.01; n ≥ 3) Index in PubMed under a CC BY license. PMID: <a href='https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03222-9'>40241046</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02993-2-12931_2025_3222_fig3_html.pngAnti-PITX1 AntibodyReversal of pulmonary vascular remodeling and pyroptosis in the hypoxic PH and SuHx PAH mouse models by overexpressing PITX1. A Construction of the PITX1 AAV5 plasmid and establishment of the SuHx PAH model under normoxic and hypoxic (10% O 2 ) conditions with the overexpression of PITX1 via AAV5 in mice. B , I Western blot: Representative images of the overexpression efficiency of PITX1 plasmid in the SuHx PAH and hypoxic groups. C , J Immunofluorescence staining: Overexpression of PITX1 in the SuHx PAH and hypoxic groups as well as statistical analysis. Scale bar: 50 μm. D , K Representative echocardiograms (left) and statistical analysis of PAAT (right 1), and PAVTI (right 2), as measured by echocardiography. E , L Right heart catheterization and the right ventricular hypertrophy index. F , G , M , N Representative images of H&E and Masson’s trichrome staining in the lung tissues of the modeled mice. Scale bar: 50 μm. H , O Western blot: Representative images as well as statistical data of NLRP3, ASC, CASP1, GSDMD-N and IL18 levels. PAAT pulmonary artery acceleration time, PAVTI pulmonary artery velocity time integral, AAV AAV5, adeno-associated virus 5. All values are presented as means ± SEMs (* p < 0.05, and ** p < 0.01; n ≥ 3) Index in PubMed under a CC BY license. PMID: <a href='https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03222-9'>40241046</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02993-2-12931_2025_3222_fig4_html.pngAnti-PITX1 AntibodyIdentification of DUSP4 as a target gene regulated by super-enhancers. A Analysis of the genes regulated by SEs under hypoxic conditions via ROSE. B ChIP-seq datasets to reveal increased levels of H3K27ac modification and the PITX1 binding sequence in DUSP4 under hypoxic conditions. C Dual-luciferase reporter assay: Luciferase activity of the DUSP4 promoter and three SE segments. D , E , F ChIP‒qPCR analysis: Performance of ChIP with anti-H3K27ac, anti-H3K4me1, and anti-PITX1 antibodies, followed by PCR with primer sequences targeting SE. Nor normoxic, Hyp hypoxic, NC noncoding nucleotides. All values are presented as means ± SEMs (* p < 0.05, ** p < 0.01, and *** p < 0.001; n ≥ 3) Index in PubMed under a CC BY license. PMID: <a href='https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03222-9'>40241046</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02993-2-12931_2025_3222_fig6_html.pngAnti-PITX1 AntibodyModulation of pyroptosis by influencing DUSP4 through its SE. A , B Construction of the dual-luciferase plasmid as well as statistical analysis of luciferase activity in mPASMCs cotransfected with PITX1 plasmid and three segments of SE-DUSP4 or corresponding mutants for 24 h. C , E RT‒qPCR: Changes in the transcript level of DUSP4 in mPASMCs at the 24-h time point under hypoxic conditions after transfection with PITX1 plasmid and three segments of SE-DUSP4 after the addition of inhibitors JQ1 and iBET. D , F Western blot: Representative images as well as statistical data of the protein expression of DUSP4 in mPASMCs at the 24-h time point under hypoxic conditions after transfection with PITX1 plasmid and three segments of SE-DUSP4 after the addition of inhibitors JQ1 and iBET. G Representative images and statistical data of positive CASP1 staining. Scale bar: 50 μm. H Western blot: Representative images as well as statistical analysis of the protein levels of GSDMD-N, IL-1β and IL-18. Nor normoxic, Hyp hypoxic. All values are presented as means ± SEMs (* p < 0.05, and ** p < 0.01; n ≥ 3) Index in PubMed under a CC BY license. PMID: <a href='https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03222-9'>40241046</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02993-2-12931_2025_3222_fig7_html.pngAnti-PITX1 AntibodyPITX1 modulating pulmonary vascular remodeling and pyroptosis through DUSP4. A Establishment of a hypoxic (10% O 2 ) mouse model with overexpression of PITX1 and knockdown of DUSP4 via AAV5. B Representative echocardiogram (left) as well as statistical analysis of PAAT (right 1) and PAVTI (right 2). C Statistical analysis of right ventricular pressure and right ventricular hypertrophy index. D , E Representative images of H&E and Masson’s trichrome staining in the lung tissue of the modeled mice. Scale bar: 50 μm. F Western blot: Representative images as well as statistical analysis of NLRP3, ASC, CASP1, GSDMD-N and IL-1β levels. G , H Immunofluorescence staining of PITX1 (red) with an antibody and DUSP4 (green) with fluorescence in situ hybridization (FISH) probes to demonstrate their colocalization in the lung tissues and mPASMCs of mice. Nuclei were counterstained with DAPI (blue). Scale bars: 25 μm, and 100 μm. I Western blot: Representative image showing the interference efficiency of siDUSP4. J LDH assay: Determination of the number of dead mPASMCs by quantifying the amount of LDH released from the membrane of damaged cells. K SEM: Morphological observation of nuclear, cytoplasmic, and membrane integrity as well as pore formation following PITX1 overexpression combined with DUSP4 knockdown, compared with those under hypoxic conditions and with PITX1 overexpression alone. Scale bar: 5 μm. L Western blot: Representative images as well as statistical data of CASP1, GSDMD-N, IL-1β, and IL-18 levels. PAAT pulmonary artery acceleration time, PAVTI pulmonary artery velocity time integral, Nor normoxic, Hyp hypoxic, NC noncoding nucleotides, shDUSP4 shRNADUSP4, AAV AAV5, adeno-associated virus 5. All values are presented as means ± SEMs (* p < 0.05, and ** p < 0.01; n ≥ 3) Index in PubMed under a CC BY license. PMID: <a href='https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03222-9'>40241046</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02993-2-pitx1-primary-antibodies-ihc-testing-1.jpgAnti-PITX1 AntibodyIHC analysis of PITX1 using anti-PITX1 antibody (A02993-2). PITX1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-PITX1 Antibody (A02993-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02993-2-pitx1-primary-antibodies-ihc-testing-2.jpgAnti-PITX1 AntibodyIHC analysis of PITX1 using anti-PITX1 antibody (A02993-2). PITX1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-PITX1 Antibody (A02993-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1301852026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301862026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301872026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301882026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301892026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301902026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301912026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301922026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301932026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301942026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301952026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301962026-03-17T05:16:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02947-2-scp2-primary-antibodies-wb-testing-1.jpgAnti-SCP2 Antibody Picoband®Western blot analysis of SCP2 using anti-SCP2 antibody (A02947-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCP2 antigen affinity purified polyclonal antibody (A02947-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SCP2 at approximately 59 kDa. The expected band size for SCP2 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02947-2-scp2-primary-antibodies-fcm-testing-1.jpgAnti-SCP2 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-SCP2 antibody (A02947-2). Overlay histogram showing PC-3 cells stained with A02947-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCP2 Antibody (A02947-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1301972026-03-17T05:16:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05860-2-srsf9-primary-antibodies-wb-testing-1.jpgAnti-SRSF9 Antibody Picoband®Western blot analysis of SRSF9 using anti-SRSF9 antibody (A05860-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRSF9 antigen affinity purified polyclonal antibody (A05860-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SRSF9 at approximately 26 kDa. The expected band size for SRSF9 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05860-2-srsf9-primary-antibodies-ihc-testing-1.jpgAnti-SRSF9 Antibody Picoband®IHC analysis of SRSF9 using anti-SRSF9 antibody (A05860-2). SRSF9 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF9 Antibody (A05860-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05860-2-srsf9-primary-antibodies-ihc-testing-2.jpgAnti-SRSF9 Antibody Picoband®IHC analysis of SRSF9 using anti-SRSF9 antibody (A05860-2). SRSF9 was detected in a paraffin-embedded section of human prostatic hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF9 Antibody (A05860-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1301982026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1301992026-03-17T05:16:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02662-2-slc16a2-primary-antibodies-wb-testing-1.jpgAnti-SLC16A2 Antibody Picoband®Western blot analysis of SLC16A2 using anti-SLC16A2 antibody (A02662-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC16A2 antigen affinity purified polyclonal antibody (A02662-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC16A2 at approximately 60 kDa. The expected band size for SLC16A2 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02662-2-slc16a2-primary-antibodies-fcm-testing-1.jpgAnti-SLC16A2 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-SLC16A2 antibody (A02662-2). Overlay histogram showing U251 cells stained with A02662-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC16A2 Antibody (A02662-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1302002026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302012026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302022026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302032026-03-16T05:08:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302042026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302052026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302062026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302072026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302092026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302102026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05614-1-triobp-primary-antibodies-wb-testing-1.jpgAnti-TRIOBP Antibody Picoband®Western blot analysis of TRIOBP using anti-TRIOBP antibody (A05614-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIOBP antigen affinity purified polyclonal antibody (A05614-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIOBP at approximately 75 kDa. The expected band size for TRIOBP is at 261 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05614-1-triobp-primary-antibodies-ihc-testing-1.jpgAnti-TRIOBP Antibody Picoband®IHC analysis of TRIOBP using anti-TRIOBP antibody (A05614-1). TRIOBP was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIOBP Antibody (A05614-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05614-1-triobp-primary-antibodies-if-testing-1.jpgAnti-TRIOBP Antibody Picoband®IF analysis of TRIOBP using anti-TRIOBP antibody (A05614-1). TRIOBP was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRIOBP Antibody (A05614-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05614-1-triobp-primary-antibodies-ip-testing-1.jpgAnti-TRIOBP Antibody Picoband®Immunoprecipitating TRIOBP in Hela whole cell lysate. Western blot analysis of TRIOBP using anti-TRIOBP antibody (A05614-1). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TRIOBP antibody in Hela whole cell lysate, Lane 3: anti-TRIOBP antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRIOBP antigen affinity purified polyclonal antibody (A05614-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TRIOBP at approximately 75 kDa. The expected band size for TRIOBP is at 261 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05614-1-triobp-primary-antibodies-fcm-testing-1.jpgAnti-TRIOBP Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-TRIOBP antibody (A05614-1). Overlay histogram showing 293T cells stained with A05614-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIOBP Antibody (A05614-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1302112026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302122026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302132026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302142026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302152026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302162026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302172026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302182026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302192026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302202026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302212026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302222026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302232026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302242026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302252026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302262026-03-16T05:08:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302272026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302282026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302292026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302302026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302312026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302322026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302332026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302342026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302352026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302362026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302372026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302382026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302392026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302402026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302412026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302422026-03-13T05:05:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302432026-04-02T05:01:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02523-2-dlx5-primary-antibodies-wb-testing-1.jpgAnti-DLX5 Antibody Picoband®Western blot analysis of DLX5 using anti-DLX5 antibody (A02523-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLX5 antigen affinity purified polyclonal antibody (A02523-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DLX5 at approximately 33 kDa. The expected band size for DLX5 is at 32 kDa. https://www.bosterbio.com/catalog/product/view/id/1302442026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302452026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302462026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302472026-04-02T05:01:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12209-2-dtymk-primary-antibodies-wb-testing-1.jpgAnti-DTYMK Antibody Picoband®Western blot analysis of DTYMK using anti-DTYMK antibody (A12209-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A49 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DTYMK antigen affinity purified polyclonal antibody (A12209-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DTYMK at approximately 24 kDa. The expected band size for DTYMK is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12209-2-dtymk-primary-antibodies-ihc-testing-1.jpgAnti-DTYMK Antibody Picoband®IHC analysis of DTYMK using anti-DTYMK antibody (A12209-2). DTYMK was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DTYMK Antibody (A12209-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12209-2-dtymk-primary-antibodies-if-testing-1.jpgAnti-DTYMK Antibody Picoband®IF analysis of DTYMK using anti-DTYMK antibody (A12209-2). DTYMK was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DTYMK Antibody (A12209-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1302482026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06043-1-dusp2-primary-antibodies-wb-testing-1.jpgAnti-DUSP2 Antibody Picoband®Western blot analysis of DUSP2 using anti-DUSP2 antibody (A06043-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP2 antigen affinity purified polyclonal antibody (A06043-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DUSP2 at approximately 34 kDa. The expected band size for DUSP2 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06043-1-dusp2-primary-antibodies-ihc-testing-1.jpgAnti-DUSP2 Antibody Picoband®IHC analysis of DUSP2 using anti-DUSP2 antibody (A06043-1). DUSP2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DUSP2 Antibody (A06043-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06043-1-dusp2-primary-antibodies-if-testing-1.jpgAnti-DUSP2 Antibody Picoband®IF analysis of DUSP2 using anti-DUSP2 antibody (A06043-1) and anti-Alpha Tubulin antibody (M03989-3). DUSP2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DUSP2 Antibody (A06043-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06043-1-dusp2-primary-antibodies-fcm-testing-1.jpgAnti-DUSP2 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-DUSP2 antibody (A06043-1). Overlay histogram showing RT4 cells stained with A06043-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DUSP2 Antibody (A06043-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1302492026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302502026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302512026-04-02T05:01:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07041-1-eif3m-primary-antibodies-wb-testing-1.jpgAnti-EIF3M Antibody Picoband®Western blot analysis of EIF3M using anti-EIF3M antibody (A07041-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3M antigen affinity purified polyclonal antibody (A07041-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EIF3M at approximately 38 kDa. The expected band size for EIF3M is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07041-1-eif3m-primary-antibodies-ihc-testing-1.jpgAnti-EIF3M Antibody Picoband®IHC analysis of EIF3M using anti-EIF3M antibody (A07041-1). EIF3M was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3M Antibody (A07041-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07041-1-eif3m-primary-antibodies-ihc-testing-2.jpgAnti-EIF3M Antibody Picoband®IHC analysis of EIF3M using anti-EIF3M antibody (A07041-1). EIF3M was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3M Antibody (A07041-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07041-1-eif3m-primary-antibodies-ihc-testing-3.jpgAnti-EIF3M Antibody Picoband®IHC analysis of EIF3M using anti-EIF3M antibody (A07041-1). EIF3M was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3M Antibody (A07041-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1302522026-03-16T05:08:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302532026-04-03T05:00:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06004-2-elovl6-primary-antibodies-wb-testing-1.jpgAnti-ELOVL6 Antibody Picoband®Western blot analysis of ELOVL6 using anti-ELOVL6 antibody (A06004-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELOVL6 antigen affinity purified polyclonal antibody (A06004-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ELOVL6 at approximately 38 kDa. The expected band size for ELOVL6 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06004-2-elovl6-primary-antibodies-fcm-testing-1.jpgAnti-ELOVL6 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-ELOVL6 antibody (A06004-2). Overlay histogram showing K562 cells stained with A06004-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ELOVL6 Antibody (A06004-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1302542026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08034-3-erlin1-primary-antibodies-wb-testing-1.jpgAnti-ERLIN1 Antibody Picoband®Western blot analysis of ERLIN1 using anti-ERLIN1 antibody (A08034-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERLIN1 antigen affinity purified polyclonal antibody (A08034-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERLIN1 at approximately 39 kDa. The expected band size for ERLIN1 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08034-3-erlin1-primary-antibodies-ip-testing-1.jpgAnti-ERLIN1 Antibody Picoband®Immunoprecipitating ERLIN1 in A549 whole cell lysate. Western blot analysis of ERLIN1 using anti-ERLIN1 antibody (A08034-3). Lane 1: A549 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-ERLIN1 antibody in A549 whole cell lysate, Lane 3: anti-ERLIN1 antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERLIN1 antigen affinity purified polyclonal antibody (A08034-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ERLIN1 at approximately 39 kDa. The expected band size for ERLIN1 is at 39 kDa. https://www.bosterbio.com/catalog/product/view/id/1302552026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302562026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302572026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302582026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302592026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07096-1-fxyd2-primary-antibodies-ihc-testing-1.jpgAnti-FXYD2 AntibodyIHC analysis of FXYD2 using anti-FXYD2 antibody (A07096-1). FXYD2 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-FXYD2 Antibody (A07096-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07096-1-fxyd2-primary-antibodies-ihc-testing-2.jpgAnti-FXYD2 AntibodyIHC analysis of FXYD2 using anti-FXYD2 antibody (A07096-1). FXYD2 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-FXYD2 Antibody (A07096-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07096-1-fxyd2-primary-antibodies-ihc-testing-3.jpgAnti-FXYD2 AntibodyIHC analysis of FXYD2 using anti-FXYD2 antibody (A07096-1). FXYD2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-FXYD2 Antibody (A07096-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07096-1-fxyd2-primary-antibodies-ihc-testing-4.jpgAnti-FXYD2 AntibodyIHC analysis of FXYD2 using anti-FXYD2 antibody (A07096-1). FXYD2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-FXYD2 Antibody (A07096-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1302602026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302612026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302622026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302632026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302642026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302652026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302662026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302672026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302682026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302692026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06112-3-gstk1-primary-antibodies-wb-testing-1.jpgAnti-GSTK1 Antibody Picoband®Western blot analysis of GSTK1 using anti-GSTK1 antibody (A06112-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTK1 antigen affinity purified polyclonal antibody (A06112-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSTK1 at approximately 26 kDa. The expected band size for GSTK1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06112-3-gstk1-primary-antibodies-ihc-testing-1.jpgAnti-GSTK1 Antibody Picoband®IHC analysis of GSTK1 using anti-GSTK1 antibody (A06112-3). GSTK1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTK1 Antibody (A06112-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06112-3-gstk1-primary-antibodies-ihc-testing-2.jpgAnti-GSTK1 Antibody Picoband®IHC analysis of GSTK1 using anti-GSTK1 antibody (A06112-3). GSTK1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTK1 Antibody (A06112-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06112-3-gstk1-primary-antibodies-ihc-testing-3.jpgAnti-GSTK1 Antibody Picoband®IHC analysis of GSTK1 using anti-GSTK1 antibody (A06112-3). GSTK1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTK1 Antibody (A06112-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06112-3-gstk1-primary-antibodies-if-testing-1.jpgAnti-GSTK1 Antibody Picoband®IF analysis of GSTK1 using anti-GSTK1 antibody (A06112-3). GSTK1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GSTK1 Antibody (A06112-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06112-3-gstk1-primary-antibodies-ip-testing-1.jpgAnti-GSTK1 Antibody Picoband®Immunoprecipitating GSTK1 in 293T whole cell lysate. Western blot analysis of GSTK1 using anti-GSTK1 antibody (A06112-3). Lane 1: 293T whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-GSTK1 antibody in 293T whole cell lysate, Lane 3: anti-GSTK1 antibody (2μg) + 293T whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GSTK1 antigen affinity purified polyclonal antibody (A06112-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GSTK1 at approximately 26 kDa. The expected band size for GSTK1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06112-3-gstk1-primary-antibodies-fcm-testing-1.jpgAnti-GSTK1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-GSTK1 antibody (A06112-3). Overlay histogram showing HepG2 cells stained with A06112-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSTK1 Antibody (A06112-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1302702026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302712026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302722026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302732026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302742026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302752026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302762026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302772026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302782026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302792026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10907-1-ifi44-primary-antibodies-wb-testing-1.jpgAnti-IFI44 Antibody Picoband®Western blot analysis of IFI44 using anti-IFI44 antibody (A10907-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFI44 antigen affinity purified polyclonal antibody (A10907-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IFI44 at approximately 50 kDa. The expected band size for IFI44 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10907-1-ifi44-primary-antibodies-fcm-testing-1.jpgAnti-IFI44 Antibody Picoband®Flow Cytometry analysis of Hepa1-6 cells using anti-IFI44 antibody (A10907-1). Overlay histogram showing Hepa1-6 cells stained with A10907-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IFI44 Antibody (A10907-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1302802026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302812026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302822026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302832026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302842026-03-16T05:08:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302852026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302862026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302872026-03-16T05:08:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04297-2-jnk-primary-antibodies-wb-testing-1.jpgAnti-JNK/MAPK10 AntibodyWestern blot analysis of JNK using anti-JNK antibody (A04297-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JNK antigen affinity purified polyclonal antibody (A04297-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for JNK at approximately 44-55 kDa. The expected band size for JNK is at 53 kDa. https://www.bosterbio.com/catalog/product/view/id/1302882026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05682-1-khk-primary-antibodies-wb-testing-1.jpgAnti-KHK AntibodyWestern blot analysis of KHK using anti-KHK antibody (A05682-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KHK antigen affinity purified polyclonal antibody (A05682-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KHK at approximately 35 kDa. The expected band size for KHK is at 35 kDa. https://www.bosterbio.com/catalog/product/view/id/1302892026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302902026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302912026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302922026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302932026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302942026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302952026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302962026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302972026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302982026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1302992026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303002026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303012026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303022026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303032026-03-16T05:08:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10476-mtfp1-primary-antibodies-wb-testing-1.jpgAnti-MTP18/MTFP1 AntibodyWestern blot analysis of MTFP1 using anti-MTFP1 antibody (A10476). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTFP1 antigen affinity purified polyclonal antibody (A10476) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MTFP1 at approximately 18 kDa. The expected band size for MTFP1 is at 18 kDa. https://www.bosterbio.com/catalog/product/view/id/1303042026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303052026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303062026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303072026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303082026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303092026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03729-2-nupr1-primary-antibodies-wb-testing-1.jpgAnti-NUPR1 Antibody Picoband®Western blot analysis of NUPR1 using anti-NUPR1 antibody (A03729-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse pancreas tissue lysates, Lane 8: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUPR1 antigen affinity purified polyclonal antibody (A03729-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NUPR1 at approximately 17 kDa. The expected band size for NUPR1 is at 9 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03729-2-nupr1-primary-antibodies-ihc-testing-1.jpgAnti-NUPR1 Antibody Picoband®IHC analysis of NUPR1 using anti-NUPR1 antibody (A03729-2). NUPR1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUPR1 Antibody (A03729-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03729-2-nupr1-primary-antibodies-ihc-testing-2.jpgAnti-NUPR1 Antibody Picoband®IHC analysis of NUPR1 using anti-NUPR1 antibody (A03729-2). NUPR1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUPR1 Antibody (A03729-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03729-2-nupr1-primary-antibodies-ihc-testing-3.jpgAnti-NUPR1 Antibody Picoband®IHC analysis of NUPR1 using anti-NUPR1 antibody (A03729-2). NUPR1 was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUPR1 Antibody (A03729-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03729-2-nupr1-primary-antibodies-ihc-testing-4.jpgAnti-NUPR1 Antibody Picoband®IHC analysis of NUPR1 using anti-NUPR1 antibody (A03729-2). NUPR1 was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUPR1 Antibody (A03729-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03729-2-nupr1-primary-antibodies-if-testing-1.jpgAnti-NUPR1 Antibody Picoband®IF analysis of NUPR1 using anti-NUPR1 antibody (A03729-2). NUPR1 was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NUPR1 Antibody (A03729-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03729-2-nupr1-primary-antibodies-if-testing-2.jpgAnti-NUPR1 Antibody Picoband®IF analysis of NUPR1 using anti-NUPR1 antibody (A03729-2). NUPR1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NUPR1 Antibody (A03729-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03729-2-nupr1-primary-antibodies-if-testing-3.jpgAnti-NUPR1 Antibody Picoband®IF analysis of NUPR1 using anti-NUPR1 antibody (A03729-2). NUPR1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NUPR1 Antibody (A03729-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03729-2-nupr1-primary-antibodies-if-testing-4.jpgAnti-NUPR1 Antibody Picoband®IF analysis of NUPR1 using anti-NUPR1 antibody (A03729-2) and anti-Beta Tubulin antibody (M01857-3). NUPR1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUPR1 Antibody (A03729-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03729-2-nupr1-primary-antibodies-fcm-testing-1.jpgAnti-NUPR1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-NUPR1 antibody (A03729-2). Overlay histogram showing HepG2 cells stained with A03729-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUPR1 Antibody (A03729-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1303102026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303112026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303122026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303132026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303142026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08686-1-parl-primary-antibodies-wb-testing-1.jpgAnti-PARL Antibody Picoband®Western blot analysis of PARL using anti-PARL antibody (A08686-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARL antigen affinity purified polyclonal antibody (A08686-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PARL at approximately 40 kDa. The expected band size for PARL is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08686-1-parl-primary-antibodies-fcm-testing-1.jpgAnti-PARL Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-PARL antibody (A08686-1). Overlay histogram showing MCF-7 cells stained with A08686-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARL Antibody (A08686-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1303152026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10408-pcif1-primary-antibodies-wb-testing-1.jpgAnti-PCIF1 Antibody Picoband®Western blot analysis of PCIF1 using anti-PCIF1 antibody (A10408). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCIF1 antigen affinity purified polyclonal antibody (A10408) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCIF1 at approximately 81 kDa. The expected band size for PCIF1 is at 81 kDa. https://www.bosterbio.com/catalog/product/view/id/1303162026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303172026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303182026-03-16T05:08:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303192026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11984-plac8-primary-antibodies-wb-testing-1.jpgAnti-PLAC8 Antibody Picoband®Western blot analysis of PLAC8 using anti-PLAC8 antibody (A11984). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAC8 antigen affinity purified polyclonal antibody (A11984) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLAC8 at approximately 13 kDa. The expected band size for PLAC8 is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11984-plac8-primary-antibodies-ihc-testing-1.jpgAnti-PLAC8 Antibody Picoband®IHC analysis of PLAC8 using anti-PLAC8 antibody (A11984). PLAC8 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLAC8 Antibody (A11984) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11984-plac8-primary-antibodies-ihc-testing-2.jpgAnti-PLAC8 Antibody Picoband®IHC analysis of PLAC8 using anti-PLAC8 antibody (A11984). PLAC8 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLAC8 Antibody (A11984) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11984-plac8-primary-antibodies-ihc-testing-3.jpgAnti-PLAC8 Antibody Picoband®IHC analysis of PLAC8 using anti-PLAC8 antibody (A11984). PLAC8 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLAC8 Antibody (A11984) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11984-plac8-primary-antibodies-if-testing-1.jpgAnti-PLAC8 Antibody Picoband®IF analysis of PLAC8 using anti-PLAC8 antibody (A11984). PLAC8 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PLAC8 Antibody (A11984) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11984-plac8-primary-antibodies-fcm-testing-1.jpgAnti-PLAC8 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-PLAC8 antibody (A11984). Overlay histogram showing THP-1 cells stained with A11984 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLAC8 Antibody (A11984, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1303202026-03-16T05:08:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07891-1-posh-primary-antibodies-wb-testing-1.jpgAnti-POSH/SH3RF1 AntibodyWestern blot analysis of POSH using anti-POSH antibody (A07891-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POSH antigen affinity purified polyclonal antibody (A07891-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POSH at approximately 100 kDa. The expected band size for POSH is at 93 kDa. https://www.bosterbio.com/catalog/product/view/id/1303212026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303222026-03-16T05:08:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05366-3-prkacb-primary-antibodies-ihc-testing-1.jpgAnti-PRKACB AntibodyIHC analysis of PRKACB using anti-PRKACB antibody (A05366-3). PRKACB was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-PRKACB Antibody (A05366-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1303232026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303242026-03-13T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02846-2-folh1-primary-antibodies-wb-testing-1.jpgAnti-PSMA/GCPII/FOLH1 AntibodyWestern blot analysis of PSMA/GCPII/FOLH1 using anti-PSMA/GCPII/FOLH1 antibody (A02846-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA/GCPII/FOLH1 antigen affinity purified polyclonal antibody (A02846-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMA/GCPII/FOLH1 at approximately 100 kDa. The expected band size for PSMA/GCPII/FOLH1 is at 81 kDa. https://www.bosterbio.com/catalog/product/view/id/1303252026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04375-1-psme3-primary-antibodies-wb-testing-1.jpgAnti-PSME3 Antibody Picoband®Western blot analysis of PSME3 using anti-PSME3 antibody (A04375-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW579 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSME3 antigen affinity purified polyclonal antibody (A04375-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSME3 at approximately 30 kDa. The expected band size for PSME3 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04375-1-psme3-primary-antibodies-ihc-testing-1.jpgAnti-PSME3 Antibody Picoband®IHC analysis of PSME3 using anti-PSME3 antibody (A04375-1). PSME3 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSME3 Antibody (A04375-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04375-1-psme3-primary-antibodies-ihc-testing-2.jpgAnti-PSME3 Antibody Picoband®IHC analysis of PSME3 using anti-PSME3 antibody (A04375-1). PSME3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSME3 Antibody (A04375-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04375-1-psme3-primary-antibodies-if-testing-1.jpgAnti-PSME3 Antibody Picoband®IF analysis of PSME3 using anti-PSME3 antibody (A04375-1) and anti-Tubulin Alpha antibody (M03989-3). PSME3 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSME3 Antibody (A04375-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1303262026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02003-2-ptgds-primary-antibodies-ihc-testing-1.jpgAnti-PTGDS Antibody Picoband®IHC analysis of PTGDS using anti-PTGDS antibody (A02003-2). PTGDS was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-PTGDS Antibody (A02003-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02003-2-ptgds-primary-antibodies-wb-testing-1.jpgAnti-PTGDS Antibody Picoband®Western blot analysis of PTGDS using anti-PTGDS antibody (A02003-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGDS antigen affinity purified polyclonal antibody (A02003-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTGDS at approximately 25 kDa. The expected band size for PTGDS is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02003-2-ptgds-primary-antibodies-fcm-testing-1.jpgAnti-PTGDS Antibody Picoband®Flow Cytometry analysis of Neuro-2a cells using anti-PTGDS antibody (A02003-2). Overlay histogram showing Neuro-2a cells stained with A02003-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGDS Antibody (A02003-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02003-2-ptgds-primary-antibodies-ihc-testing-2.jpgAnti-PTGDS Antibody Picoband®IHC analysis of PTGDS using anti-PTGDS antibody (A02003-2). PTGDS was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-PTGDS Antibody (A02003-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1303272026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303282026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02520-1-pura-primary-antibodies-wb-testing-1.jpgAnti-PURA Antibody Picoband®Western blot analysis of PURA using anti-PURA antibody (A02520-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PURA antigen affinity purified polyclonal antibody (A02520-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PURA at approximately 42 kDa. The expected band size for PURA is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02520-1-pura-primary-antibodies-ihc-testing-1.jpgAnti-PURA Antibody Picoband®IHC analysis of PURA using anti-PURA antibody (A02520-1). PURA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PURA Antibody (A02520-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02520-1-pura-primary-antibodies-ihc-testing-2.jpgAnti-PURA Antibody Picoband®IHC analysis of PURA using anti-PURA antibody (A02520-1). PURA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PURA Antibody (A02520-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02520-1-pura-primary-antibodies-ihc-testing-3.jpgAnti-PURA Antibody Picoband®IHC analysis of PURA using anti-PURA antibody (A02520-1). PURA was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PURA Antibody (A02520-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02520-1-pura-primary-antibodies-ihc-testing-4.jpgAnti-PURA Antibody Picoband®IHC analysis of PURA using anti-PURA antibody (A02520-1). PURA was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PURA Antibody (A02520-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02520-1-pura-primary-antibodies-if-testing-1.jpgAnti-PURA Antibody Picoband®IF analysis of PURA using anti-PURA antibody (A02520-1) and anti-Alpha Tubulin antibody (M03989-3). PURA was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PURA Antibody (A02520-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1303292026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303302026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303312026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01580-4-rad52-primary-antibodies-wb-testing-1.jpgAnti-RAD52 Antibody Picoband®Western blot analysis of RAD52 using anti-RAD52 antibody (A01580-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD52 antigen affinity purified polyclonal antibody (A01580-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAD52 at approximately 46 kDa. The expected band size for RAD52 is at 46 kDa. https://www.bosterbio.com/catalog/product/view/id/1303322026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303332026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303342026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04439-1-rbm10-primary-antibodies-wb-testing-1.jpgAnti-RBM10 Antibody Picoband®Western blot analysis of RBM10 using anti-RBM10 antibody (A04439-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human MDA-MB-231 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM10 antigen affinity purified polyclonal antibody (A04439-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBM10 at approximately 100-120 kDa. The expected band size for RBM10 is at 104 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04439-1-rbm10-primary-antibodies-ihc-testing-1.jpgAnti-RBM10 Antibody Picoband®IHC analysis of RBM10 using anti-RBM10 antibody (A04439-1). RBM10 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM10 Antibody (A04439-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04439-1-rbm10-primary-antibodies-ihc-testing-2.jpgAnti-RBM10 Antibody Picoband®IHC analysis of RBM10 using anti-RBM10 antibody (A04439-1). RBM10 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM10 Antibody (A04439-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04439-1-rbm10-primary-antibodies-ihc-testing-3.jpgAnti-RBM10 Antibody Picoband®IHC analysis of RBM10 using anti-RBM10 antibody (A04439-1). RBM10 was detected in a paraffin-embedded section of human lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM10 Antibody (A04439-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04439-1-rbm10-primary-antibodies-ihc-testing-4.jpgAnti-RBM10 Antibody Picoband®IHC analysis of RBM10 using anti-RBM10 antibody (A04439-1). RBM10 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBM10 Antibody (A04439-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04439-1-rbm10-primary-antibodies-if-testing-1.jpgAnti-RBM10 Antibody Picoband®IF analysis of RBM10 using anti-RBM10 antibody (A04439-1) and anti-Alpha Tubulin antibody (M03989-3). RBM10 was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RBM10 Antibody (A04439-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1303352026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303362026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03130-2-recql4-primary-antibodies-wb-testing-1.jpgAnti-RECQL4 Antibody Picoband®Western blot analysis of RECQL4 using anti-RECQL4 antibody (A03130-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RECQL4 antigen affinity purified polyclonal antibody (A03130-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RECQL4 at approximately 150 kDa. The expected band size for RECQL4 is at 133 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03130-2-recql4-primary-antibodies-if-testing-1.jpgAnti-RECQL4 Antibody Picoband®IF analysis of RECQL4 using anti-RECQL4 antibody (A03130-2). RECQL4 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RECQL4 Antibody (A03130-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1303372026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303382026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303392026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303402026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303412026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303422026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303432026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303442026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303452026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303462026-03-13T05:05:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303472026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303482026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303492026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303502026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303512026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303522026-03-17T05:17:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02625-1-gucy2d-primary-antibodies-wb-testing-1.jpgAnti-GUCY2D Antibody Picoband®Western blot analysis of GUCY2D using anti-GUCY2D antibody (A02625-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GUCY2D antigen affinity purified polyclonal antibody (A02625-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GUCY2D at approximately 120 kDa. The expected band size for GUCY2D is at 120 kDa. https://www.bosterbio.com/catalog/product/view/id/1303532026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303542026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303552026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303562026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303572026-03-16T05:08:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303582026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303592026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303602026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303612026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303622026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303632026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303642026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303652026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303662026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303672026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303682026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02782-2-lpar1-primary-antibodies-wb-testing-1.jpgAnti-EDG2/LPAR1 Antibody Picoband®Western blot analysis of EDG2/LPAR1 using anti-EDG2/LPAR1 antibody (A02782-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDG2/LPAR1 antigen affinity purified polyclonal antibody (A02782-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EDG2/LPAR1 at approximately 41 kDa. The expected band size for EDG2/LPAR1 is at 41 kDa. https://www.bosterbio.com/catalog/product/view/id/1303692026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12172-2-eefsec-primary-antibodies-wb-testing-1.jpgAnti-EEFSEC Antibody Picoband®Western blot analysis of EEFSEC using anti-EEFSEC antibody (A12172-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEFSEC antigen affinity purified polyclonal antibody (A12172-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EEFSEC at approximately 70 kDa. The expected band size for EEFSEC is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12172-2-eefsec-primary-antibodies-fcm-testing-1.jpgAnti-EEFSEC Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-EEFSEC antibody (A12172-2). Overlay histogram showing 293T cells stained with A12172-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EEFSEC Antibody (A12172-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1303702026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303712026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00572-efs-primary-antibodies-wb-testing-1.jpgAnti-EFS Antibody Picoband®Western blot analysis of EFS using anti-EFS antibody (A00572). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat ovary tissue lysates, Lane 5: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EFS antigen affinity purified polyclonal antibody (A00572) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EFS at approximately 85 kDa. The expected band size for EFS is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00572-efs-primary-antibodies-ip-testing-1.jpgAnti-EFS Antibody Picoband®Immunoprecipitating (IP) EFS in SiHa whole cell lysate. Western blot analysis of EFS using anti-EFS antibody (A00572); Lane 1: SiHa whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-EFS antibody in SiHa whole cell lysate; Lane 3: anti-EFS antibody (2μg) + SiHa whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EFS antigen affinity purified polyclonal antibody (A00572) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for EFS at approximately 85 kDa. The expected band size for EFS is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00572-efs-primary-antibodies-fcm-testing-1.jpgAnti-EFS Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-EFS antibody (A00572). Overlay histogram showing SH-SY5Y cells stained with A00572 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EFS Antibody (A00572, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1303722026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303732026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303742026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303752026-04-02T05:01:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08919-1-eml1-primary-antibodies-wb-testing-1.jpgAnti-EML1 Antibody Picoband®Western blot analysis of EML1 using anti-EML1 antibody (A08919-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EML1 antigen affinity purified polyclonal antibody (A08919-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EML1 at approximately 90 kDa. The expected band size for EML1 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08919-1-eml1-primary-antibodies-ihc-testing-1.jpgAnti-EML1 Antibody Picoband®IHC analysis of EML1 using anti-EML1 antibody (A08919-1). EML1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EML1 Antibody (A08919-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08919-1-eml1-primary-antibodies-ihc-testing-2.jpgAnti-EML1 Antibody Picoband®IHC analysis of EML1 using anti-EML1 antibody (A08919-1). EML1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EML1 Antibody (A08919-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08919-1-eml1-primary-antibodies-ihc-testing-3.jpgAnti-EML1 Antibody Picoband®IHC analysis of EML1 using anti-EML1 antibody (A08919-1). EML1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EML1 Antibody (A08919-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1303762026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303772026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303782026-04-02T05:01:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11668-enpp4-primary-antibodies-wb-testing-1.jpgAnti-ENPP4 Antibody Picoband®Western blot analysis of ENPP4 using anti-ENPP4 antibody (A11668). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENPP4 antigen affinity purified polyclonal antibody (A11668) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ENPP4 at approximately 48 kDa. The expected band size for ENPP4 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11668-enpp4-primary-antibodies-ihc-testing-1.jpgAnti-ENPP4 Antibody Picoband®IHC analysis of ENPP4 using anti-ENPP4 antibody (A11668). ENPP4 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENPP4 Antibody (A11668) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11668-enpp4-primary-antibodies-fcm-testing-1.jpgAnti-ENPP4 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-ENPP4 antibody (A11668). Overlay histogram showing 293T cells stained with A11668 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ENPP4 Antibody (A11668, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1303792026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303802026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303812026-03-16T05:08:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303822026-04-02T05:01:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11141-1-exosc7-primary-antibodies-wb-testing-1.jpgAnti-EXOSC7 Antibody Picoband®Western blot analysis of EXOSC7 using anti-EXOSC7 antibody (A11141-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EXOSC7 antigen affinity purified polyclonal antibody (A11141-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EXOSC7 at approximately 37 kDa. The expected band size for EXOSC7 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11141-1-exosc7-primary-antibodies-ihc-testing-1.jpgAnti-EXOSC7 Antibody Picoband®IHC analysis of EXOSC7 using anti-EXOSC7 antibody (A11141-1). EXOSC7 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EXOSC7 Antibody (A11141-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11141-1-exosc7-primary-antibodies-ihc-testing-2.jpgAnti-EXOSC7 Antibody Picoband®IHC analysis of EXOSC7 using anti-EXOSC7 antibody (A11141-1). EXOSC7 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EXOSC7 Antibody (A11141-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11141-1-exosc7-primary-antibodies-ip-testing-1.jpgAnti-EXOSC7 Antibody Picoband®Immunoprecipitating EXOSC7 in Hela whole cell lysate. Western blot analysis of EXOSC7 using anti-EXOSC7 antibody (A11141-1). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-EXOSC7 antibody in Hela whole cell lysate, Lane 3: anti-EXOSC7 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EXOSC7 antigen affinity purified polyclonal antibody (A11141-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EXOSC7 at approximately 37 kDa. The expected band size for EXOSC7 is at 32 kDa. https://www.bosterbio.com/catalog/product/view/id/1303832026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07231-3-exosc9-primary-antibodies-wb-testing-1.jpgAnti-EXOSC9 Antibody Picoband®Western blot analysis of EXOSC9 using anti-EXOSC9 antibody (A07231-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EXOSC9 antigen affinity purified polyclonal antibody (A07231-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EXOSC9 at approximately 70 kDa. The expected band size for EXOSC9 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07231-3-exosc9-primary-antibodies-ihc-testing-1.jpgAnti-EXOSC9 Antibody Picoband®IHC analysis of EXOSC9 using anti-EXOSC9 antibody (A07231-3). EXOSC9 was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EXOSC9 Antibody (A07231-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07231-3-exosc9-primary-antibodies-if-testing-1.jpgAnti-EXOSC9 Antibody Picoband®IF analysis of EXOSC9 using anti-EXOSC9 antibody (A07231-3) and anti-Alpha Tubulin antibody (M03989-3). EXOSC9 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EXOSC9 Antibody (A07231-3) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07231-3-exosc9-primary-antibodies-fcm-testing-1.jpgAnti-EXOSC9 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-EXOSC9 antibody (A07231-3). Overlay histogram showing 293T cells stained with A07231-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EXOSC9 Antibody (A07231-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1303842026-03-16T05:08:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303852026-03-16T05:08:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303862026-03-16T05:08:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06429-2-fbxo31-primary-antibodies-wb-testing-1.jpgAnti-FBXO31 AntibodyWestern blot analysis of FBXO31 using anti-FBXO31 antibody (A06429-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXO31 antigen affinity purified polyclonal antibody (A06429-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXO31 at approximately 70 kDa. The expected band size for FBXO31 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06429-2-fbxo31-primary-antibodies-ihc-testing-1.jpgAnti-FBXO31 AntibodyIHC analysis of FBXO31 using anti-FBXO31 antibody (A06429-2). FBXO31 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-FBXO31 Antibody (A06429-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1303872026-03-16T05:08:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303882026-03-16T05:08:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303892026-03-16T05:08:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303902026-03-16T05:08:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303912026-03-16T05:08:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303922026-03-16T05:08:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303932026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303942026-03-16T05:08:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303952026-03-16T05:08:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303962026-03-16T05:08:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303972026-03-16T05:08:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1303992026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1304002026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10710-2-ddx24-primary-antibodies-wb-testing-1.jpgAnti-DDX24 Antibody Picoband®Western blot analysis of DDX24 using anti-DDX24 antibody (A10710-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX24 antigen affinity purified polyclonal antibody (A10710-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX24 at approximately 120 kDa. The expected band size for DDX24 is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10710-2-ddx24-primary-antibodies-ihc-testing-1.jpgAnti-DDX24 Antibody Picoband®IHC analysis of DDX24 using anti-DDX24 antibody (A10710-2). DDX24 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX24 Antibody (A10710-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10710-2-ddx24-primary-antibodies-if-testing-1.jpgAnti-DDX24 Antibody Picoband®IF analysis of DDX24 using anti-DDX24 antibody (A10710-2) and anti-Alpha Tubulin antibody (M03989-3). DDX24 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDX24 Antibody (A10710-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10710-2-ddx24-primary-antibodies-ip-testing-1.jpgAnti-DDX24 Antibody Picoband®Immunoprecipitating DDX24 in Hela whole cell lysate. Western blot analysis of DDX24 using anti-DDX24 antibody (A10710-2). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DDX24 antibody in Hela whole cell lysate, Lane 3: anti-DDX24 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DDX24 antigen affinity purified polyclonal antibody (A10710-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DDX24 at approximately 120 kDa. The expected band size for DDX24 is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10710-2-ddx24-primary-antibodies-fcm-testing-1.jpgAnti-DDX24 Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-DDX24 antibody (A10710-2). Overlay histogram showing HeLa cells stained with A10710-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX24 Antibody (A10710-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304012026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11278-ddx28-primary-antibodies-wb-testing-1.jpgAnti-DDX28 Antibody Picoband®Western blot analysis of DDX28 using anti-DDX28 antibody (A11278). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX28 antigen affinity purified polyclonal antibody (A11278) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX28 at approximately 60 kDa. The expected band size for DDX28 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11278-ddx28-primary-antibodies-if-testing-1.jpgAnti-DDX28 Antibody Picoband®IF analysis of DDX28 using anti-DDX28 antibody (A11278) and anti-Alpha Tubulin antibody (M03989-3). DDX28 was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDX28 Antibody (A11278) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1304022026-03-16T05:08:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1304032026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1304042026-03-16T05:08:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1304052026-03-16T05:08:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1304062026-04-02T05:01:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03731-1-dhh-primary-antibodies-wb-testing-1.jpgAnti-DHH Antibody Picoband®Western blot analysis of DHH using anti-DHH antibody (A03731-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHH antigen affinity purified polyclonal antibody (A03731-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHH at approximately 44 kDa. The expected band size for DHH is at 44 kDa. https://www.bosterbio.com/catalog/product/view/id/1304072026-03-16T05:08:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07990-2-dhrs4-primary-antibodies-wb-testing-1.jpgAnti-DHRS4 AntibodyWestern blot analysis of DHRS4 using anti-DHRS4 antibody (A07990-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHRS4 antigen affinity purified polyclonal antibody (A07990-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHRS4 at approximately 30 kDa. The expected band size for DHRS4 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07990-2-dhrs4-primary-antibodies-ihc-testing-1.jpgAnti-DHRS4 AntibodyIHC analysis of DHRS4 using anti-DHRS4 antibody (A07990-2). DHRS4 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-DHRS4 Antibody (A07990-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07990-2-dhrs4-primary-antibodies-ihc-testing-2.jpgAnti-DHRS4 AntibodyIHC analysis of DHRS4 using anti-DHRS4 antibody (A07990-2). DHRS4 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-DHRS4 Antibody (A07990-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07990-2-dhrs4-primary-antibodies-ihc-testing-3.jpgAnti-DHRS4 AntibodyIHC analysis of DHRS4 using anti-DHRS4 antibody (A07990-2). DHRS4 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-DHRS4 Antibody (A07990-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07990-2-dhrs4-primary-antibodies-ihc-testing-4.jpgAnti-DHRS4 AntibodyIHC analysis of DHRS4 using anti-DHRS4 antibody (A07990-2). DHRS4 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-DHRS4 Antibody (A07990-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1304082026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09027-1-dhtkd1-primary-antibodies-wb-testing-1.jpgAnti-DHTKD1 Antibody Picoband®Western blot analysis of DHTKD1 using anti-DHTKD1 antibody (A09027-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHTKD1 antigen affinity purified polyclonal antibody (A09027-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHTKD1 at approximately 103 kDa. The expected band size for DHTKD1 is at 103 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09027-1-dhtkd1-primary-antibodies-fcm-testing-1.jpgAnti-DHTKD1 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-DHTKD1 antibody (A09027-1). Overlay histogram showing MCF-7 cells stained with A09027-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DHTKD1 Antibody (A09027-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304092026-03-16T05:08:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1304102026-03-16T05:08:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1304112026-03-16T05:08:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1304122026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07956-4-dok2-primary-antibodies-wb-testing-1.jpgAnti-DOK2 Antibody Picoband®Western blot analysis of DOK2 using anti-DOK2 antibody (A07956-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOK2 antigen affinity purified polyclonal antibody (A07956-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DOK2 at approximately 50 kDa. The expected band size for DOK2 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07956-4-dok2-primary-antibodies-ihc-testing-1.jpgAnti-DOK2 Antibody Picoband®IHC analysis of DOK2 using anti-DOK2 antibody (A07956-4). DOK2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DOK2 Antibody (A07956-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07956-4-dok2-primary-antibodies-ip-testing-1.jpgAnti-DOK2 Antibody Picoband®Immunoprecipitating DOK2 in HEL whole cell lysate. Western blot analysis of DOK2 using anti-DOK2 antibody (A07956-4). Lane 1: HEL whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DOK2 antibody in HEL whole cell lysate, Lane 3: anti-DOK2 antibody (2μg) + HEL whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DOK2 antigen affinity purified polyclonal antibody (A07956-4) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DOK2 at approximately 50 kDa. The expected band size for DOK2 is at 45 kDa. https://www.bosterbio.com/catalog/product/view/id/1304132026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09019-2-dok3-primary-antibodies-wb-testing-1.jpgAnti-DOK3 Antibody Picoband®Western blot analysis of DOK3 using anti-DOK3 antibody (A09019-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Ramos whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOK3 antigen affinity purified polyclonal antibody (A09019-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DOK3 at approximately 53 kDa. The expected band size for DOK3 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09019-2-dok3-primary-antibodies-ihc-testing-1.jpgAnti-DOK3 Antibody Picoband®IHC analysis of DOK3 using anti-DOK3 antibody (A09019-2). DOK3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DOK3 Antibody (A09019-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09019-2-dok3-primary-antibodies-fcm-testing-1.jpgAnti-DOK3 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-DOK3 antibody (A09019-2). Overlay histogram showing K562 cells stained with A09019-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DOK3 Antibody (A09019-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304142026-03-16T05:08:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1304152026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08338-2-dpp7-primary-antibodies-wb-testing-1.jpgAnti-DPP7 Antibody Picoband®Western blot analysis of DPP7 using anti-DPP7 antibody (A08338-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPP7 antigen affinity purified polyclonal antibody (A08338-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DPP7 at approximately 50 kDa. The expected band size for DPP7 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08338-2-dpp7-primary-antibodies-ihc-testing-1.jpgAnti-DPP7 Antibody Picoband®IHC analysis of DPP7 using anti-DPP7 antibody (A08338-2). DPP7 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DPP7 Antibody (A08338-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08338-2-dpp7-primary-antibodies-ihc-testing-2.jpgAnti-DPP7 Antibody Picoband®IHC analysis of DPP7 using anti-DPP7 antibody (A08338-2). DPP7 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DPP7 Antibody (A08338-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08338-2-dpp7-primary-antibodies-fcm-testing-1.jpgAnti-DPP7 Antibody Picoband®Flow Cytometry analysis of U937 cells using anti-DPP7 antibody (A08338-2). Overlay histogram showing U937 cells stained with A08338-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DPP7 Antibody (A08338-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304162026-03-16T05:08:59+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rbpms2a-b-antibody-azq6dh13-boster.html2026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dh13-rbpms2a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RBPMS2a/b Antibody Picoband®Western blot analysis of RBPMS2a/b using anti-RBPMS2a/b antibody (AZQ6DH13). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates。After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBPMS2a/b antigen affinity purified polyclonal antibody (AZQ6DH13) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBPMS2a/b at approximately 30 kDa. The expected band size for RBPMS2a/b is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rrm1-antibody-azp79732-boster.html2026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp79732-rrm1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RRM1 Antibody Picoband®Western blot analysis of RRM1 using anti-RRM1 antibody (AZP79732). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates。After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRM1 antigen affinity purified polyclonal antibody (AZP79732) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RRM1 at approximately 90 kDa. The expected band size for RRM1 is at 90 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sephs1-antibody-azq7zw38-boster.html2026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zw38-sephs1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SEPHS1 Antibody Picoband®Western blot analysis of SEPHS1 using anti-SEPHS1 antibody (AZQ7ZW38). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPHS1 antigen affinity purified polyclonal antibody (AZQ7ZW38) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SEPHS1 at approximately 50 kDa. The expected band size for SEPHS1 is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sf1-antibody-aza0a0r4ibt0-boster.html2026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4ibt0-sf1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SF1 Antibody Picoband®Western blot analysis of SF1 using anti-SF1 antibody (AZA0A0R4IBT0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF1 antigen affinity purified polyclonal antibody (AZA0A0R4IBT0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SF1 at approximately 68 kDa. The expected band size for SF1 is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sf3a3-antibody-azq6drk2-boster.html2026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6drk2-sf3a3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SF3A3 Antibody Picoband®Western blot analysis of SF3A3 using anti-SF3A3 antibody (AZQ6DRK2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3A3 antigen affinity purified polyclonal antibody (AZQ6DRK2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SF3A3 at approximately 59 kDa. The expected band size for SF3A3 is at 59 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sf3b4-antibody-azq6nwb3-boster.html2026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nwb3-sf3b4-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SF3B4 Antibody Picoband®Western blot analysis of SF3B4 using anti-SF3B4 antibody (AZQ6NWB3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3B4 antigen affinity purified polyclonal antibody (AZQ6NWB3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SF3B4 at approximately 49 kDa. The expected band size for SF3B4 is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sgce-antibody-azq6dhh1-boster.html2026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dhh1-sgce-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SGCE Antibody Picoband®Western blot analysis of SGCE using anti-SGCE antibody (AZQ6DHH1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGCE antigen affinity purified polyclonal antibody (AZQ6DHH1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SGCE at approximately 50 kDa. The expected band size for SGCE is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-smarcb1a-b-antibody-azq5u379-boster.html2026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5u379-smarcb1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SMARCB1a/b Antibody Picoband®Western blot analysis of SMARCB1a/b using anti-SMARCB1a/b antibody (AZQ5U379). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCB1a/b antigen affinity purified polyclonal antibody (AZQ5U379) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMARCB1a/b at approximately 44 kDa. The expected band size for SMARCB1a/b is at 44 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-smarce1-antibody-aza0a8m2bb53-boster.html2026-03-17T05:17:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bb53-smarce1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SMARCE1 Antibody Picoband®Western blot analysis of SMARCE1 using anti-SMARCE1 antibody (AZA0A8M2BB53). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCE1 antigen affinity purified polyclonal antibody (AZA0A8M2BB53) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMARCE1 at approximately 55 kDa. The expected band size for SMARCE1 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-snrpd3-3l-antibody-azq6iq56-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iq56-snrpd3-3i-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SNRPD3/3l Antibody Picoband®Western blot analysis of SNRPD3/3I using anti-SNRPD3/3I antibody (AZQ6IQ56). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRPD3/3I antigen affinity purified polyclonal antibody (AZQ6IQ56) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNRPD3/3I at approximately 18 kDa. The expected band size for SNRPD3/3I is at 14 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sox1a-b-antibody-azq6dgl6-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dgl6-sox1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SOX1a/b Antibody Picoband®Western blot analysis of SOX1a/b using anti-SOX1a/b antibody (AZQ6DGL6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX1a/b antigen affinity purified polyclonal antibody (AZQ6DGL6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SOX1a/b at approximately 39 kDa. The expected band size for SOX1a/b is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-srsf1a-b-antibody-azq7sxp4-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxp4-srsf1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SRSF1a/b Antibody Picoband®Western blot analysis of SRSF1a/b using anti-SRSF1a/b antibody (AZQ7SXP4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRSF1a/b antigen affinity purified polyclonal antibody (AZQ7SXP4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SRSF1a/b at approximately 33 kDa. The expected band size for SRSF1a/b is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxp4-srsf1a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish SRSF1a/b Antibody Picoband®IHC analysis of SRSF1a/b using anti-SRSF1a/b antibody (AZQ7SXP4). SRSF1a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF1a/b Antibody (AZQ7SXP4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxp4-srsf1a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish SRSF1a/b Antibody Picoband®IHC analysis of SRSF1a/b using anti-SRSF1a/b antibody (AZQ7SXP4). SRSF1a/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF1a/b Antibody (AZQ7SXP4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxp4-srsf1a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish SRSF1a/b Antibody Picoband®IHC analysis of SRSF1a/b using anti-SRSF1a/b antibody (AZQ7SXP4). SRSF1a/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF1a/b Antibody (AZQ7SXP4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxp4-srsf1a-b-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish SRSF1a/b Antibody Picoband®IHC analysis of SRSF1a/b using anti-SRSF1a/b antibody (AZQ7SXP4). SRSF1a/b was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF1a/b Antibody (AZQ7SXP4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxp4-srsf1a-b-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish SRSF1a/b Antibody Picoband®IHC analysis of SRSF1a/b using anti-SRSF1a/b antibody (AZQ7SXP4). SRSF1a/b was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF1a/b Antibody (AZQ7SXP4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxp4-srsf1a-b-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish SRSF1a/b Antibody Picoband®IHC analysis of SRSF1a/b using anti-SRSF1a/b antibody (AZQ7SXP4). SRSF1a/b was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF1a/b Antibody (AZQ7SXP4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-srsf3a-b-antibody-azq801u3-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq801u3-srsf3a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SRSF3a/b Antibody Picoband®Western blot analysis of SRSF3a/b using anti-SRSF3a/b antibody (AZQ801U3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRSF3a/b antigen affinity purified polyclonal antibody (AZQ801U3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SRSF3a/b at approximately 17 kDa. The expected band size for SRSF3a/b is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq801u3-srsf3a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish SRSF3a/b Antibody Picoband®IHC analysis of SRSF3a/b using anti-SRSF3a/b antibody (AZQ801U3). SRSF3a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF3a/b Antibody (AZQ801U3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq801u3-srsf3a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish SRSF3a/b Antibody Picoband®IHC analysis of SRSF3a/b using anti-SRSF3a/b antibody (AZQ801U3). SRSF3a/b was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF3a/b Antibody (AZQ801U3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq801u3-srsf3a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish SRSF3a/b Antibody Picoband®IHC analysis of SRSF3a/b using anti-SRSF3a/b antibody (AZQ801U3). SRSF3a/b was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF3a/b Antibody (AZQ801U3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-stip1-antibody-azq5rkm3-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5rkm3-stip1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish STIP1 Antibody Picoband®Western blot analysis of STIP1 using anti-STIP1 antibody (AZQ5RKM3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIP1 antigen affinity purified polyclonal antibody (AZQ5RKM3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STIP1 at approximately 63 kDa. The expected band size for STIP1 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5rkm3-stip1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish STIP1 Antibody Picoband®IHC analysis of STIP1 using anti-STIP1 antibody (AZQ5RKM3). STIP1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STIP1 Antibody (AZQ5RKM3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5rkm3-stip1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish STIP1 Antibody Picoband®IHC analysis of STIP1 using anti-STIP1 antibody (AZQ5RKM3). STIP1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STIP1 Antibody (AZQ5RKM3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5rkm3-stip1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish STIP1 Antibody Picoband®IHC analysis of STIP1 using anti-STIP1 antibody (AZQ5RKM3). STIP1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STIP1 Antibody (AZQ5RKM3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5rkm3-stip1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish STIP1 Antibody Picoband®IHC analysis of STIP1 using anti-STIP1 antibody (AZQ5RKM3). STIP1 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STIP1 Antibody (AZQ5RKM3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5rkm3-stip1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish STIP1 Antibody Picoband®IHC analysis of STIP1 using anti-STIP1 antibody (AZQ5RKM3). STIP1 was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STIP1 Antibody (AZQ5RKM3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sub1a-b-antibody-azq504b1-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq504b1-sub1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SUB1a/b Antibody Picoband®Western blot analysis of SUB1a/b using anti-SUB1a/b antibody (AZQ504B1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUB1a/b antigen affinity purified polyclonal antibody (AZQ504B1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUB1a/b at approximately 18 kDa. The expected band size for SUB1a/b is at 14 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-suclg1-antibody-azq66i58-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq66i58-suclg1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SUCLG1 Antibody Picoband®Western blot analysis of SUCLG1 using anti-SUCLG1 antibody (AZQ66I58). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLG1 antigen affinity purified polyclonal antibody (AZQ66I58) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUCLG1 at approximately 36 kDa. The expected band size for SUCLG1 is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sumo2-3-3b-3l-antibody-azq6dhl4-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dhl4-sumo2-3-3b-3i-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SUMO2,3,3b,3l Antibody Picoband®Western blot analysis of SUMO2/3/3b/3I using anti-SUMO2/3/3b/3I antibody (AZQ6DHL4). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUMO2/3/3b/3I antigen affinity purified polyclonal antibody (AZQ6DHL4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUMO2/3/3b/3I at approximately 18 kDa. The expected band size for SUMO2/3/3b/3I is at 12 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tardbpa-b-antibody-aza0a8m9psz1-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9psz1-tardbpa-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TARDBPa/b Antibody Picoband®Western blot analysis of TARDBPa/b using anti-TARDBPa/b antibody (AZA0A8M9PSZ1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TARDBPa/b antigen affinity purified polyclonal antibody (AZA0A8M9PSZ1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TARDBPa/b at approximately 45 kDa. The expected band size for TARDBPa/b is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tdrd3-antibody-azq6nyg6-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nyg6-tdrd3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TDRD3 Antibody Picoband®Western blot analysis of TDRD3 using anti-TDRD3 antibody (AZQ6NYG6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDRD3 antigen affinity purified polyclonal antibody (AZQ6NYG6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TDRD3 at approximately 73 kDa. The expected band size for TDRD3 is at 73 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tead1a-b-antibody-aza0a8m3b730-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3b730-tead1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TEAD1a/b Antibody Picoband®Western blot analysis of TEAD1a/b using anti-TEAD1a/b antibody (AZA0A8M3B730). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEAD1a/b antigen affinity purified polyclonal antibody (AZA0A8M3B730) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TEAD1a/b at approximately 48 kDa. The expected band size for TEAD1a/b is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tollip-antibody-azq7zv43-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zv43-tollip-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TOLLIP Antibody Picoband®Western blot analysis of TOLLIP using anti-TOLLIP antibody (AZQ7ZV43). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOLLIP antigen affinity purified polyclonal antibody (AZQ7ZV43) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TOLLIP at approximately 30 kDa. The expected band size for TOLLIP is at 30 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-trir-antibody-aza0a0r4ip71-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4ip71-trir-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TRIR Antibody Picoband®Western blot analysis of TRIR using anti-TRIR antibody (AZA0A0R4IP71). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIR antigen affinity purified polyclonal antibody (AZA0A0R4IP71) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIR at approximately 24 kDa. The expected band size for TRIR is at 18 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tsg101a-b-antibody-azq6iq70-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iq70-tsg101a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TSG101a/b Antibody Picoband®Western blot analysis of TSG101a/b using anti-TSG101a/b antibody (AZQ6IQ70). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101a/b antigen affinity purified polyclonal antibody (AZQ6IQ70) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TSG101a/b at approximately 44 kDa. The expected band size for TSG101a/b is at 44 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-uba3-antibody-azq7zvx6-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvx6-uba3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish UBA3 Antibody Picoband®Western blot analysis of UBA3 using anti-UBA3 antibody (AZQ7ZVX6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA3 antigen affinity purified polyclonal antibody (AZQ7ZVX6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBA3 at approximately 52 kDa. The expected band size for UBA3 is at 52 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ube2ka-b-antibody-azq5bla9-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5bla9-ube2ka-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish UBE2Ka/b Antibody Picoband®Western blot analysis of UBE2Ka/b using anti-UBE2Ka/b antibody (AZQ5BLA9). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2Ka/b antigen affinity purified polyclonal antibody (AZQ5BLA9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBE2Ka/b at approximately 22 kDa. The expected band size for UBE2Ka/b is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ube2na-b-antibody-azq803j2-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq803j2-ube2na-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish UBE2Na/b Antibody Picoband®Western blot analysis of UBE2Na/b using anti-UBE2Na/b antibody (AZQ803J2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2Na/b antigen affinity purified polyclonal antibody (AZQ803J2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBE2Na/b at approximately 17 kDa. The expected band size for UBE2Na/b is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq803j2-ube2na-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish UBE2Na/b Antibody Picoband®IHC analysis of UBE2Na/b using anti-UBE2Na/b antibody (AZQ803J2). UBE2Na/b was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBE2Na/b Antibody (AZQ803J2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-upf1-antibody-azf1rcy6-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1rcy6-upf1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish UPF1 Antibody Picoband®Western blot analysis of UPF1 using anti-UPF1 antibody (AZF1RCY6). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UPF1 antigen affinity purified polyclonal antibody (AZF1RCY6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UPF1 at approximately 130 kDa. The expected band size for UPF1 is at 124 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-wdr5-antibody-azq7ztx2-boster.html2026-03-17T05:17:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7ztx2-wdr5-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish WDR5 Antibody Picoband®Western blot analysis of WDR5 using anti-WDR5 antibody (AZQ7ZTX2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR5 antigen affinity purified polyclonal antibody (AZQ7ZTX2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for WDR5 at approximately 36 kDa. The expected band size for WDR5 is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ybx1-antibody-azb5de31-boster.html2026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb5de31-ybx1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish YBX1 Antibody Picoband®Western blot analysis of YBX1 using anti-YBX1 antibody (AZB5DE31). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YBX1 antigen affinity purified polyclonal antibody (AZB5DE31) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for YBX1 at approximately 50 kDa. The expected band size for YBX1 is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ywhae1-2-antibody-azq7zw20-boster.html2026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zw20-ywhae1-2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish YWHAE1/2 Antibody Picoband®Western blot analysis of YWHAE1/2 using anti-YWHAE1/2 antibody (AZQ7ZW20). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAE1/2 antigen affinity purified polyclonal antibody (AZQ7ZW20) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for YWHAE1/2 at approximately 32 kDa. The expected band size for YWHAE1/2 is at 29 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-zmynd8-antibody-aza0a0r4isb9-boster.html2026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4isb9-zmynd8-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ZMYND8 Antibody Picoband®Western blot analysis of ZMYND8 using anti-ZMYND8 antibody (AZA0A0R4ISB9). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZMYND8 antigen affinity purified polyclonal antibody (AZA0A0R4ISB9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ZMYND8 at approximately 150 kDa. The expected band size for ZMYND8 is at 132 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nova1-antibody-aza0a8m2bck7-boster.html2026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bck7-nova1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NOVA1 Antibody Picoband®Western blot analysis of NOVA1 using anti-NOVA1 antibody (AZA0A8M2BCK7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOVA1 antigen affinity purified polyclonal antibody (AZA0A8M2BCK7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NOVA1 at approximately 52 kDa. The expected band size for NOVA1 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bck7-nova1-primary-antibodies-if-testing-1.jpgAnti-Zebrafish NOVA1 Antibody Picoband®IF analysis of NOVA1 using anti-NOVA1 antibody (AZA0A8M2BCK7). NOVA1 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NOVA1 Antibody (AZA0A8M2BCK7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-stxbp1a-b-antibody-aza0a0r4iqm9-boster.html2026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4iqm9-stxbp1a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish STXBP1a/b Antibody Picoband®Western blot analysis of STXBP1a/b using anti-STXBP1a/b antibody (AZA0A0R4IQM9). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STXBP1a/b antigen affinity purified polyclonal antibody (AZA0A0R4IQM9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STXBP1a/b at approximately 68 kDa. The expected band size for STXBP1a/b is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ube2ia-b-antibody-azq9w6h5-boster.html2026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9w6h5-ube2ia-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish UBE2Ia/b Antibody Picoband®Western blot analysis of UBE2Ia/b using anti-UBE2Ia/b antibody (AZQ9W6H5). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2Ia/b antigen affinity purified polyclonal antibody (AZQ9W6H5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBE2Ia/b at approximately 18 kDa. The expected band size for UBE2Ia/b is at 18 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-usp7-antibody-azf1qus9-boster.html2026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qus9-usp7-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish USP7 Antibody Picoband®Western blot analysis of USP7 using anti-USP7 antibody (AZF1QUS9). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP7 antigen affinity purified polyclonal antibody (AZF1QUS9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for USP7 at approximately 140 kDa. The expected band size for USP7 is at 128 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-suz12a-b-antibody-azq6dc03-boster.html2026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dc03-suz12a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SUZ12a/b AntibodyWestern blot analysis of SUZ12a/b using anti-SUZ12a/b antibody (AZQ6DC03). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUZ12a/b antigen affinity purified polyclonal antibody (AZQ6DC03) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUZ12a/b at approximately 90 kDa. The expected band size for SUZ12a/b is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dc03-suz12a-b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish SUZ12a/b AntibodyIHC analysis of SUZ12a/b using anti-SUZ12a/b antibody (AZQ6DC03). SUZ12a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUZ12a/b Antibody (AZQ6DC03) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dc03-suz12a-b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish SUZ12a/b AntibodyIHC analysis of SUZ12a/b using anti-SUZ12a/b antibody (AZQ6DC03). SUZ12a/b was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUZ12a/b Antibody (AZQ6DC03) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dc03-suz12a-b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish SUZ12a/b AntibodyIHC analysis of SUZ12a/b using anti-SUZ12a/b antibody (AZQ6DC03). SUZ12a/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUZ12a/b Antibody (AZQ6DC03) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ttc28-antibody-aza0a8m6z8c1-boster.html2026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6z8c1-ttc28-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish TTC28 AntibodyIHC analysis of TTC28 using anti-TTC28 antibody (AZA0A8M6Z8C1). TTC28 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC28 Antibody (AZA0A8M6Z8C1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6z8c1-ttc28-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish TTC28 AntibodyIHC analysis of TTC28 using anti-TTC28 antibody (AZA0A8M6Z8C1). TTC28 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC28 Antibody (AZA0A8M6Z8C1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6z8c1-ttc28-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish TTC28 AntibodyIHC analysis of TTC28 using anti-TTC28 antibody (AZA0A8M6Z8C1). TTC28 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC28 Antibody (AZA0A8M6Z8C1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ttc7b-antibody-aza0a8m3ajw5-boster.html2026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3ajw5-ttc7b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish TTC7B AntibodyIHC analysis of TTC7B using anti-TTC7B antibody (AZA0A8M3AJW5). TTC7B was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC7B Antibody (AZA0A8M3AJW5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3ajw5-ttc7b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish TTC7B AntibodyIHC analysis of TTC7B using anti-TTC7B antibody (AZA0A8M3AJW5). TTC7B was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTC7B Antibody (AZA0A8M3AJW5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-vps53-antibody-aze7fag0-boster.html2026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7fag0-vps53-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish VPS53 AntibodyIHC analysis of VPS53 using anti-VPS53 antibody (AZE7FAG0). VPS53 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VPS53 Antibody (AZE7FAG0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7fag0-vps53-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish VPS53 AntibodyIHC analysis of VPS53 using anti-VPS53 antibody (AZE7FAG0). VPS53 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VPS53 Antibody (AZE7FAG0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7fag0-vps53-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish VPS53 AntibodyIHC analysis of VPS53 using anti-VPS53 antibody (AZE7FAG0). VPS53 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VPS53 Antibody (AZE7FAG0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1304562026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00596-1-pdc-primary-antibodies-wb-testing-1.jpgAnti-PDC Antibody Picoband®Western blot analysis of PDC using anti-PDC antibody (A00596-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDC antigen affinity purified polyclonal antibody (A00596-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDC at approximately 28 kDa. The expected band size for PDC is at 28 kDa. https://www.bosterbio.com/catalog/product/view/id/1304572026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10337-2-ncdn-primary-antibodies-wb-testing-1.jpgAnti-NCDN Antibody Picoband®Western blot analysis of NCDN using anti-NCDN antibody (A10337-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCDN antigen affinity purified polyclonal antibody (A10337-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NCDN at approximately 79 kDa. The expected band size for NCDN is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10337-2-ncdn-primary-antibodies-ihc-testing-1.jpgAnti-NCDN Antibody Picoband®IHC analysis of NCDN using anti-NCDN antibody (A10337-2). NCDN was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCDN Antibody (A10337-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10337-2-ncdn-primary-antibodies-ihc-testing-2.jpgAnti-NCDN Antibody Picoband®IHC analysis of NCDN using anti-NCDN antibody (A10337-2). NCDN was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCDN Antibody (A10337-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10337-2-ncdn-primary-antibodies-ihc-testing-3.jpgAnti-NCDN Antibody Picoband®IHC analysis of NCDN using anti-NCDN antibody (A10337-2). NCDN was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCDN Antibody (A10337-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10337-2-ncdn-primary-antibodies-if-testing-1.jpgAnti-NCDN Antibody Picoband®IF analysis of NCDN using anti-NCDN antibody (A10337-2). NCDN was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NCDN Antibody (A10337-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10337-2-ncdn-primary-antibodies-if-testing-2.jpgAnti-NCDN Antibody Picoband®IF analysis of NCDN using anti-NCDN antibody (A10337-2). NCDN was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NCDN Antibody (A10337-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10337-2-ncdn-primary-antibodies-fcm-testing-1.jpgAnti-NCDN Antibody Picoband®Flow Cytometry analysis of SIHA cells using anti-NCDN antibody (A10337-2). Overlay histogram showing SIHA cells stained with A10337-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCDN Antibody (A10337-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10337-2-ncdn-primary-antibodies-ihc-testing-4.jpgAnti-NCDN Antibody Picoband®IHC analysis of NCDN using anti-NCDN antibody (A10337-2). NCDN was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCDN Antibody (A10337-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1304582026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13773-1-nyap2-primary-antibodies-wb-testing-1.jpgAnti-NYAP2 Antibody Picoband®Western blot analysis of NYAP2 using anti-NYAP2 antibody (A13773-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NYAP2 antigen affinity purified polyclonal antibody (A13773-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NYAP2 at approximately 80 kDa. The expected band size for NYAP2 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13773-1-nyap2-primary-antibodies-fcm-testing-1.jpgAnti-NYAP2 Antibody Picoband®Flow Cytometry analysis of SIHA cells using anti-NYAP2 antibody (A13773-1). Overlay histogram showing SIHA cells stained with A13773-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NYAP2 Antibody (A13773-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304592026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11651-2-cbx6-primary-antibodies-wb-testing-1.jpgAnti-CBX6 Antibody Picoband®Western blot analysis of CBX6 using anti-CBX6 antibody (A11651-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cel lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cel lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBX6 antigen affinity purified polyclonal antibody (A11651-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CBX6 at approximately 44 kDa. The expected band size for CBX6 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11651-2-cbx6-primary-antibodies-if-testing-1.jpgAnti-CBX6 Antibody Picoband®IF analysis of CBX6 using anti-CBX6 antibody (A11651-2) and anti-Beta Tubulin antibody (M01857-3). CBX6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CBX6 Antibody (A11651-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11651-2-cbx6-primary-antibodies-fcm-testing-1.jpgAnti-CBX6 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-CBX6 antibody (A11651-2). Overlay histogram showing RT4 cells stained with A11651-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CBX6 Antibody (A11651-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304602026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07975-1-maml3-primary-antibodies-wb-testing-1.jpgAnti-MAML3 Antibody Picoband®Western blot analysis of MAML3 using anti-MAML3 antibody (A07975-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAML3 antigen affinity purified polyclonal antibody (A07975-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAML3 at approximately 140-150 kDa. The expected band size for MAML3 is at 122 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07975-1-maml3-primary-antibodies-ihc-testing-1.jpgAnti-MAML3 Antibody Picoband®IHC analysis of MAML3 using anti-MAML3 antibody (A07975-1). MAML3 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAML3 Antibody (A07975-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07975-1-maml3-primary-antibodies-ihc-testing-2.jpgAnti-MAML3 Antibody Picoband®IHC analysis of MAML3 using anti-MAML3 antibody (A07975-1). MAML3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAML3 Antibody (A07975-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07975-1-maml3-primary-antibodies-if-testing-1.jpgAnti-MAML3 Antibody Picoband®IF analysis of MAML3 using anti-MAML3 antibody (A07975-1). MAML3 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MAML3 Antibody (A07975-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07975-1-maml3-primary-antibodies-if-testing-2.jpgAnti-MAML3 Antibody Picoband®IF analysis of MAML3 using anti-MAML3 antibody (A07975-1). MAML3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MAML3 Antibody (A07975-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07975-1-maml3-primary-antibodies-if-testing-3.jpgAnti-MAML3 Antibody Picoband®IF analysis of MAML3 using anti-MAML3 antibody (A07975-1) and anti-Beta Tubulin antibody (M01857-3). MAML3 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MAML3 Antibody (A07975-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07975-1-maml3-primary-antibodies-fcm-testing-1.jpgAnti-MAML3 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-MAML3 antibody (A07975-1). Overlay histogram showing 293T cells stained with A07975-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAML3 Antibody (A07975-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304612026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13028-1-msto1-primary-antibodies-wb-testing-1.jpgAnti-MSTO1 Antibody Picoband®Western blot analysis of MSTO1 using anti-MSTO1 antibody (A13028-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSTO1 antigen affinity purified polyclonal antibody (A13028-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MSTO1 at approximately 62 kDa. The expected band size for MSTO1 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13028-1-msto1-primary-antibodies-ihc-testing-1.jpgAnti-MSTO1 Antibody Picoband®IHC analysis of MSTO1 using anti-MSTO1 antibody (A13028-1). MSTO1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSTO1 Antibody (A13028-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13028-1-msto1-primary-antibodies-ihc-testing-2.jpgAnti-MSTO1 Antibody Picoband®IHC analysis of MSTO1 using anti-MSTO1 antibody (A13028-1). MSTO1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSTO1 Antibody (A13028-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13028-1-msto1-primary-antibodies-ihc-testing-3.jpgAnti-MSTO1 Antibody Picoband®IHC analysis of MSTO1 using anti-MSTO1 antibody (A13028-1). MSTO1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSTO1 Antibody (A13028-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13028-1-msto1-primary-antibodies-ihc-testing-4.jpgAnti-MSTO1 Antibody Picoband®IHC analysis of MSTO1 using anti-MSTO1 antibody (A13028-1). MSTO1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSTO1 Antibody (A13028-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13028-1-msto1-primary-antibodies-if-testing-1.jpgAnti-MSTO1 Antibody Picoband®IF analysis of MSTO1 using anti-MSTO1 antibody (A13028-1). MSTO1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MSTO1 Antibody (A13028-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13028-1-msto1-primary-antibodies-fcm-testing-1.jpgAnti-MSTO1 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-MSTO1 antibody (A13028-1). Overlay histogram showing 293T cells stained with A13028-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSTO1 Antibody (A13028-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304622026-03-17T05:17:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07594-gkn1-primary-antibodies-wb-testing-1.jpgAnti-GKN1 Antibody Picoband®Western blot analysis of GKN1 using anti-GKN1 antibody (A07594). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GKN1 antigen affinity purified polyclonal antibody (A07594) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GKN1 at approximately 18 kDa. The expected band size for GKN1 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07594-gkn1-primary-antibodies-ihc-testing-1.jpgAnti-GKN1 Antibody Picoband®IHC analysis of GKN1 using anti-GKN1 antibody (A07594). GKN1 was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GKN1 Antibody (A07594) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07594-gkn1-primary-antibodies-ihc-testing-2.jpgAnti-GKN1 Antibody Picoband®IHC analysis of GKN1 using anti-GKN1 antibody (A07594). GKN1 was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GKN1 Antibody (A07594) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07594-gkn1-primary-antibodies-ihc-testing-3.jpgAnti-GKN1 Antibody Picoband®IHC analysis of GKN1 using anti-GKN1 antibody (A07594). GKN1 was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GKN1 Antibody (A07594) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1304632026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03636-2-nlrp6-primary-antibodies-wb-testing-1.jpgAnti-NLRP6 Antibody Picoband®Western blot analysis of NLRP6 using anti-NLRP6 antibody (A03636-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP6 antigen affinity purified polyclonal antibody (A03636-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NLRP6 at approximately 105 kDa. The expected band size for NLRP6 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03636-2-nlrp6-primary-antibodies-fcm-testing-1.jpgAnti-NLRP6 Antibody Picoband®Flow Cytometry analysis of HL-60 cells using anti-NLRP6 antibody (A03636-2). Overlay histogram showing HL-60 cells stained with A03636-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRP6 Antibody (A03636-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304642026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06191-1-nfu1-primary-antibodies-wb-testing-1.jpgAnti-NFU1 Antibody Picoband®Western blot analysis of NFU1 using anti-NFU1 antibody (A06191-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFU1 antigen affinity purified polyclonal antibody (A06191-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NFU1 at approximately 28 kDa. The expected band size for NFU1 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06191-1-nfu1-primary-antibodies-if-testing-1.jpgAnti-NFU1 Antibody Picoband®IF analysis of NFU1 using anti-NFU1 antibody (A06191-1) and anti-Beta Tubulin antibody (M01857-3). NFU1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NFU1 Antibody (A06191-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06191-1-nfu1-primary-antibodies-fcm-testing-1.jpgAnti-NFU1 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-NFU1 antibody (A06191-1). Overlay histogram showing A549 cells stained with A06191-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFU1 Antibody (A06191-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304652026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08704-1-immp2l-primary-antibodies-wb-testing-1.jpgAnti-IMMP2L Antibody Picoband®Western blot analysis of IMMP2L using anti-IMMP2L antibody (A08704-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMMP2L antigen affinity purified polyclonal antibody (A08704-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IMMP2L at approximately 23 kDa. The expected band size for IMMP2L is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08704-1-immp2l-primary-antibodies-if-testing-1.jpgAnti-IMMP2L Antibody Picoband®IF analysis of IMMP2L using anti-IMMP2L antibody (A08704-1) and anti-Beta Tubulin antibody (M01857-3). IMMP2L was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-IMMP2L Antibody (A08704-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08704-1-immp2l-primary-antibodies-fcm-testing-1.jpgAnti-IMMP2L Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-IMMP2L antibody (A08704-1). Overlay histogram showing Jurkat cells stained with A08704-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IMMP2L Antibody (A08704-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304662026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10545-1-parp16-primary-antibodies-ihc-testing-1.jpgAnti-PARP16 AntibodyIHC analysis of PARP16 using anti-PARP16 antibody (A10545-1). PARP16 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARP16 Antibody (A10545-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10545-1-parp16-primary-antibodies-ihc-testing-2.jpgAnti-PARP16 AntibodyIHC analysis of PARP16 using anti-PARP16 antibody (A10545-1). PARP16 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARP16 Antibody (A10545-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10545-1-parp16-primary-antibodies-if-testing-1.jpgAnti-PARP16 AntibodyIF analysis of PARP16 using anti-PARP16 antibody (A10545-1). PARP16 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PARP16 Antibody (A10545-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10545-1-parp16-primary-antibodies-fcm-testing-1.jpgAnti-PARP16 AntibodyFlow Cytometry analysis of HL-60 cells using anti-PARP16 antibody (A10545-1). Overlay histogram showing HL-60 cells stained with A10545-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARP16 Antibody (A10545-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304672026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04187-2-mybbp1a-primary-antibodies-wb-testing-1.jpgAnti-MYBBP1A Antibody Picoband®Western blot analysis of MYBBP1A using anti-MYBBP1A antibody (A04187-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat RH35 whole cell lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse NIH/3T3 tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYBBP1A antigen affinity purified polyclonal antibody (A04187-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYBBP1A at approximately 150 kDa. The expected band size for MYBBP1A is at 149 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04187-2-mybbp1a-primary-antibodies-if-testing-1.jpgAnti-MYBBP1A Antibody Picoband®IF analysis of MYBBP1A using anti-MYBBP1A antibody (A04187-2) and anti-Beta Tubulin antibody (M01857-3). MYBBP1A was detected in an immunocytochemical section of NIH/3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MYBBP1A Antibody (A04187-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1304682026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05501-1-ncapg-primary-antibodies-wb-testing-1.jpgAnti-NCAPG Antibody Picoband®Western blot analysis of NCAPG using anti-NCAPG antibody (A05501-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAPG antigen affinity purified polyclonal antibody (A05501-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NCAPG at approximately 131 kDa. The expected band size for NCAPG is at 114 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05501-1-ncapg-primary-antibodies-ihc-testing-1.jpgAnti-NCAPG Antibody Picoband®IHC analysis of NCAPG using anti-NCAPG antibody (A05501-1). NCAPG was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCAPG Antibody (A05501-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05501-1-ncapg-primary-antibodies-if-testing-1.jpgAnti-NCAPG Antibody Picoband®IF analysis of NCAPG using anti-NCAPG antibody (A05501-1) and anti-Beta Tubulin antibody (M01857-3). NCAPG was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NCAPG Antibody (A05501-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05501-1-ncapg-primary-antibodies-fcm-testing-1.jpgAnti-NCAPG Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-NCAPG antibody (A05501-1). Overlay histogram showing 293T cells stained with A05501-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCAPG Antibody (A05501-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304692026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04660-1-nek9-primary-antibodies-wb-testing-1.jpgAnti-NEK9 Antibody Picoband®Western blot analysis of NEK9 using anti-NEK9 antibody (A04660-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEK9 antigen affinity purified polyclonal antibody (A04660-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NEK9 at approximately 120 kDa. The expected band size for NEK9 is at 107 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04660-1-nek9-primary-antibodies-ihc-testing-1.jpgAnti-NEK9 Antibody Picoband®IHC analysis of NEK9 using anti-NEK9 antibody (A04660-1). NEK9 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEK9 Antibody (A04660-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04660-1-nek9-primary-antibodies-ihc-testing-2.jpgAnti-NEK9 Antibody Picoband®IHC analysis of NEK9 using anti-NEK9 antibody (A04660-1). NEK9 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEK9 Antibody (A04660-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04660-1-nek9-primary-antibodies-ihc-testing-3.jpgAnti-NEK9 Antibody Picoband®IHC analysis of NEK9 using anti-NEK9 antibody (A04660-1). NEK9 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEK9 Antibody (A04660-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04660-1-nek9-primary-antibodies-if-testing-1.jpgAnti-NEK9 Antibody Picoband®IF analysis of NEK9 using anti-NEK9 antibody (A04660-1). NEK9 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NEK9 Antibody (A04660-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04660-1-nek9-primary-antibodies-if-testing-2.jpgAnti-NEK9 Antibody Picoband®IF analysis of NEK9 using anti-NEK9 antibody (A04660-1). NEK9 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NEK9 Antibody (A04660-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04660-1-nek9-primary-antibodies-fcm-testing-1.jpgAnti-NEK9 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-NEK9 antibody (A04660-1). Overlay histogram showing HepG2 cells stained with A04660-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEK9 Antibody (A04660-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304702026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14002-3-nek5-primary-antibodies-wb-testing-1.jpgAnti-NEK5 Antibody Picoband®Western blot analysis of NEK5 using anti-NEK5 antibody (A14002-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse ovary tissue lysates, Lane 6: mouse LLC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEK5 antigen affinity purified polyclonal antibody (A14002-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NEK5 at approximately 80 kDa. The expected band size for NEK5 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14002-3-nek5-primary-antibodies-ihc-testing-1.jpgAnti-NEK5 Antibody Picoband®IHC analysis of NEK5 using anti-NEK5 antibody (A14002-3). NEK5 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEK5 Antibody (A14002-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14002-3-nek5-primary-antibodies-ihc-testing-2.jpgAnti-NEK5 Antibody Picoband®IHC analysis of NEK5 using anti-NEK5 antibody (A14002-3). NEK5 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NEK5 Antibody (A14002-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14002-3-nek5-primary-antibodies-if-testing-1.jpgAnti-NEK5 Antibody Picoband®IF analysis of NEK5 using anti-NEK5 antibody (A14002-3) and anti-Beta Tubulin antibody (M01857-3). NEK5 was detected in an immunocytochemical section of NIH/3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NEK5 Antibody (A14002-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1304712026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08994-2-stxbp3-primary-antibodies-wb-testing-1.jpgAnti-STXBP3 Antibody Picoband®Western blot analysis of STXBP3 using anti-STXBP3 antibody (A08994-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STXBP3 antigen affinity purified polyclonal antibody (A08994-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STXBP3 at approximately 68 kDa. The expected band size for STXBP3 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08994-2-stxbp3-primary-antibodies-ihc-testing-1.jpgAnti-STXBP3 Antibody Picoband®IHC analysis of STXBP3 using anti-STXBP3 antibody (A08994-2). STXBP3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STXBP3 Antibody (A08994-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08994-2-stxbp3-primary-antibodies-ihc-testing-2.jpgAnti-STXBP3 Antibody Picoband®IHC analysis of STXBP3 using anti-STXBP3 antibody (A08994-2). STXBP3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STXBP3 Antibody (A08994-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08994-2-stxbp3-primary-antibodies-ihc-testing-3.jpgAnti-STXBP3 Antibody Picoband®IHC analysis of STXBP3 using anti-STXBP3 antibody (A08994-2). STXBP3 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STXBP3 Antibody (A08994-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08994-2-stxbp3-primary-antibodies-if-testing-1.jpgAnti-STXBP3 Antibody Picoband®IF analysis of STXBP3 using anti-STXBP3 antibody (A08994-2). STXBP3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-STXBP3 Antibody (A08994-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08994-2-stxbp3-primary-antibodies-fcm-testing-1.jpgAnti-STXBP3 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-STXBP3 antibody (A08994-2). Overlay histogram showing MCF-7 cells stained with A08994-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STXBP3 Antibody (A08994-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304722026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12788-suco-primary-antibodies-wb-testing-1.jpgAnti-SUCO Antibody Picoband®Western blot analysis of SUCO using anti-SUCO antibody (A12788). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCO antigen affinity purified polyclonal antibody (A12788) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUCO at approximately 250 kDa. The expected band size for SUCO is at 139 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12788-suco-primary-antibodies-ihc-testing-1.jpgAnti-SUCO Antibody Picoband®IHC analysis of SUCO using anti-SUCO antibody (A12788). SUCO was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCO Antibody (A12788) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12788-suco-primary-antibodies-ihc-testing-2.jpgAnti-SUCO Antibody Picoband®IHC analysis of SUCO using anti-SUCO antibody (A12788). SUCO was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCO Antibody (A12788) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12788-suco-primary-antibodies-ihc-testing-3.jpgAnti-SUCO Antibody Picoband®IHC analysis of SUCO using anti-SUCO antibody (A12788). SUCO was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCO Antibody (A12788) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12788-suco-primary-antibodies-ihc-testing-4.jpgAnti-SUCO Antibody Picoband®IHC analysis of SUCO using anti-SUCO antibody (A12788). SUCO was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SUCO Antibody (A12788) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12788-suco-primary-antibodies-if-testing-1.jpgAnti-SUCO Antibody Picoband®IF analysis of SUCO using anti-SUCO antibody (A12788). SUCO was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SUCO Antibody (A12788) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12788-suco-primary-antibodies-fcm-testing-1.jpgAnti-SUCO Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-SUCO antibody (A12788). Overlay histogram showing PC-3 cells stained with A12788 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUCO Antibody (A12788, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304732026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03601-1-sun1-primary-antibodies-wb-testing-1.jpgAnti-SUN1 Antibody Picoband®Western blot analysis of SUN1 using anti-SUN1 antibody (A03601-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUN1 antigen affinity purified polyclonal antibody (A03601-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUN1 at approximately 100 kDa. The expected band size for SUN1 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03601-1-sun1-primary-antibodies-fcm-testing-1.jpgAnti-SUN1 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-SUN1 antibody (A03601-1). Overlay histogram showing Jurkat cells stained with A03601-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUN1 Antibody (A03601-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304742026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10237-2-sun5-primary-antibodies-wb-testing-1.jpgAnti-SUN5 Antibody Picoband®Western blot analysis of SUN5 using anti-SUN5 antibody (A10237-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUN5 antigen affinity purified polyclonal antibody (A10237-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUN5 at approximately 50 kDa. The expected band size for SUN5 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10237-2-sun5-primary-antibodies-fcm-testing-1.jpgAnti-SUN5 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-SUN5 antibody (A10237-2). Overlay histogram showing PC-3 cells stained with A10237-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUN5 Antibody (A10237-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304752026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05199-1-supt16h-primary-antibodies-wb-testing-1.jpgAnti-SUPT16H Antibody Picoband®Western blot analysis of SUPT16H using anti-SUPT16H antibody (A05199-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUPT16H antigen affinity purified polyclonal antibody (A05199-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUPT16H at approximately 140 kDa. The expected band size for SUPT16H is at 120 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05199-1-supt16h-primary-antibodies-if-testing-1.jpgAnti-SUPT16H Antibody Picoband®IF analysis of SUPT16H using anti-SUPT16H antibody (A05199-1) and anti-Beta Tubulin antibody (M01857-3). SUPT16H was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SUPT16H Antibody (A05199-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05199-1-supt16h-primary-antibodies-fcm-testing-1.jpgAnti-SUPT16H Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-SUPT16H antibody (A05199-1). Overlay histogram showing MCF-7 cells stained with A05199-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUPT16H Antibody (A05199-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304762026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-wb-testing-1.jpgAnti-TAF3 Antibody Picoband®Western blot analysis of TAF3 using anti-TAF3 antibody (A07129-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF3 antigen affinity purified polyclonal antibody (A07129-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAF3 at approximately 140 kDa. The expected band size for TAF3 is at 104 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-ihc-testing-1.jpgAnti-TAF3 Antibody Picoband®IHC analysis of TAF3 using anti-TAF3 antibody (A07129-1). TAF3 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAF3 Antibody (A07129-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-ihc-testing-2.jpgAnti-TAF3 Antibody Picoband®IHC analysis of TAF3 using anti-TAF3 antibody (A07129-1). TAF3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAF3 Antibody (A07129-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-ihc-testing-3.jpgAnti-TAF3 Antibody Picoband®IHC analysis of TAF3 using anti-TAF3 antibody (A07129-1). TAF3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAF3 Antibody (A07129-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-ihc-testing-4.jpgAnti-TAF3 Antibody Picoband®IHC analysis of TAF3 using anti-TAF3 antibody (A07129-1). TAF3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAF3 Antibody (A07129-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-ihc-testing-5.jpgAnti-TAF3 Antibody Picoband®IHC analysis of TAF3 using anti-TAF3 antibody (A07129-1). TAF3 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAF3 Antibody (A07129-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-ihc-testing-6.jpgAnti-TAF3 Antibody Picoband®IHC analysis of TAF3 using anti-TAF3 antibody (A07129-1). TAF3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAF3 Antibody (A07129-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-ihc-testing-7.jpgAnti-TAF3 Antibody Picoband®IHC analysis of TAF3 using anti-TAF3 antibody (A07129-1). TAF3 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAF3 Antibody (A07129-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-ihc-testing-8.jpgAnti-TAF3 Antibody Picoband®IHC analysis of TAF3 using anti-TAF3 antibody (A07129-1). TAF3 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAF3 Antibody (A07129-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-ihc-testing-9.jpgAnti-TAF3 Antibody Picoband®IHC analysis of TAF3 using anti-TAF3 antibody (A07129-1). TAF3 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAF3 Antibody (A07129-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-ihc-testing-10.jpgAnti-TAF3 Antibody Picoband®IHC analysis of TAF3 using anti-TAF3 antibody (A07129-1). TAF3 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAF3 Antibody (A07129-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-if-testing-1.jpgAnti-TAF3 Antibody Picoband®IF analysis of TAF3 using anti-TAF3 antibody (A07129-1) and anti-Beta Tubulin antibody (M01857-3). TAF3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TAF3 Antibody (A07129-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07129-1-taf3-primary-antibodies-fcm-testing-1.jpgAnti-TAF3 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-TAF3 antibody (A07129-1). Overlay histogram showing 293T cells stained with A07129-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAF3 Antibody (A07129-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304772026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05993-1-taf6-primary-antibodies-wb-testing-1.jpgAnti-TAF6 Antibody Picoband®Western blot analysis of TAF6 using anti-TAF6 antibody (A05993-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF6 antigen affinity purified polyclonal antibody (A05993-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAF6 at approximately 73 kDa. The expected band size for TAF6 is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05993-1-taf6-primary-antibodies-if-testing-1.jpgAnti-TAF6 Antibody Picoband®IF analysis of TAF6 using anti-TAF6 antibody (A05993-1) and anti-Beta Tubulin antibody (M01857-3). TAF6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TAF6 Antibody (A05993-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05993-1-taf6-primary-antibodies-ip-testing-1.jpgAnti-TAF6 Antibody Picoband®Immunoprecipitating TAF6 in MCF-7 whole cell lysate. Western blot analysis of TAF6 using anti-TAF6 antibody (A05993-1). Lane 1: MCF-7 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TAF6 antibody in MCF-7 whole cell lysate, Lane 3: anti-TAF6 antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TAF6 antigen affinity purified polyclonal antibody (A05993-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TAF6 at approximately 73 kDa. The expected band size for TAF6 is at 73 kDa. https://www.bosterbio.com/catalog/product/view/id/1304782026-03-17T05:17:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07896-1-tas1r1-primary-antibodies-wb-testing-1.jpgAnti-TAS1R1 Antibody Picoband®Western blot analysis of TAS1R1 using anti-TAS1R1 antibody (A07896-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAS1R1 antigen affinity purified polyclonal antibody (A07896-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAS1R1 at approximately 140 kDa. The expected band size for TAS1R1 is at 93 kDa. https://www.bosterbio.com/catalog/product/view/id/1304792026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07052-1-ntn4-primary-antibodies-wb-testing-1.jpgAnti-NTN4 Antibody Picoband®Western blot analysis of NTN4 using anti-NTN4 antibody (A07052-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTN4 antigen affinity purified polyclonal antibody (A07052-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NTN4 at approximately 70 kDa. The expected band size for NTN4 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07052-1-ntn4-primary-antibodies-ihc-testing-1.jpgAnti-NTN4 Antibody Picoband®IHC analysis of NTN4 using anti-NTN4 antibody (A07052-1). NTN4 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NTN4 Antibody (A07052-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07052-1-ntn4-primary-antibodies-fcm-testing-1.jpgAnti-NTN4 Antibody Picoband®Flow Cytometry analysis of SIHA cells using anti-NTN4 antibody (A07052-1). Overlay histogram showing SIHA cells stained with A07052-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NTN4 Antibody (A07052-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304802026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05915-1-gypb-primary-antibodies-wb-testing-1.jpgAnti-GYPB Antibody Picoband®Western blot analysis of GYPB using anti-GYPB antibody (A05915-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GYPB antigen affinity purified polyclonal antibody (A05915-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GYPB at approximately 17 kDa. The expected band size for GYPB is at 10 kDa. https://www.bosterbio.com/catalog/product/view/id/1304812026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08915-nrxn2-primary-antibodies-wb-testing-1.jpgAnti-NRXN2 Antibody Picoband®Western blot analysis of NRXN2 using anti-NRXN2 antibody (A08915). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRXN2 antigen affinity purified polyclonal antibody (A08915) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NRXN2 at approximately 185 kDa. The expected band size for NRXN2 is at 185 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08915-nrxn2-primary-antibodies-wb-testing-2.jpgAnti-NRXN2 Antibody Picoband®Western blot analysis of NRXN2 using anti-NRXN2 antibody (A08915). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions. Lane 1: mouse plasma tissue lysates, Lane 2: mouse cerebellum tissue lysates, After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRXN2 antigen affinity purified polyclonal antibody (A08915) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NRXN2 at approximately 185 kDa. The expected band size for NRXN2 is at 185 kDa. https://www.bosterbio.com/catalog/product/view/id/1304822026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06908-2-entpd5-primary-antibodies-wb-testing-1.jpgAnti-ENTPD5 Antibody Picoband®Western blot analysis of ENTPD5 using anti-ENTPD5 antibody (A06908-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENTPD5 antigen affinity purified polyclonal antibody (A06908-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ENTPD5 at approximately 48 kDa. The expected band size for ENTPD5 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06908-2-entpd5-primary-antibodies-ihc-testing-1.jpgAnti-ENTPD5 Antibody Picoband®IHC analysis of ENTPD5 using anti-ENTPD5 antibody (A06908-2). ENTPD5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENTPD5 Antibody (A06908-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06908-2-entpd5-primary-antibodies-ihc-testing-2.jpgAnti-ENTPD5 Antibody Picoband®IHC analysis of ENTPD5 using anti-ENTPD5 antibody (A06908-2). ENTPD5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENTPD5 Antibody (A06908-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06908-2-entpd5-primary-antibodies-ihc-testing-3.jpgAnti-ENTPD5 Antibody Picoband®IHC analysis of ENTPD5 using anti-ENTPD5 antibody (A06908-2). ENTPD5 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENTPD5 Antibody (A06908-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06908-2-entpd5-primary-antibodies-ihc-testing-4.jpgAnti-ENTPD5 Antibody Picoband®IHC analysis of ENTPD5 using anti-ENTPD5 antibody (A06908-2). ENTPD5 was detected in a paraffin-embedded section of mouse adrenal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENTPD5 Antibody (A06908-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06908-2-entpd5-primary-antibodies-ihc-testing-5.jpgAnti-ENTPD5 Antibody Picoband®IHC analysis of ENTPD5 using anti-ENTPD5 antibody (A06908-2). ENTPD5 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENTPD5 Antibody (A06908-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06908-2-entpd5-primary-antibodies-if-testing-1.jpgAnti-ENTPD5 Antibody Picoband®IF analysis of ENTPD5 using anti-ENTPD5 antibody (A06908-2). ENTPD5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ENTPD5 Antibody (A06908-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06908-2-entpd5-primary-antibodies-fcm-testing-1.jpgAnti-ENTPD5 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-ENTPD5 antibody (A06908-2). Overlay histogram showing RT4 cells stained with A06908-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ENTPD5 Antibody (A06908-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304832026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07088-1-evc2-primary-antibodies-wb-testing-1.jpgAnti-EVC2 Antibody Picoband®Western blot analysis of EVC2 using anti-EVC2 antibody (A07088-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EVC2 antigen affinity purified polyclonal antibody (A07088-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EVC2 at approximately 153 kDa. The expected band size for EVC2 is at 148 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07088-1-evc2-primary-antibodies-ihc-testing-1.jpgAnti-EVC2 Antibody Picoband®IHC analysis of EVC2 using anti-EVC2 antibody (A07088-1). EVC2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EVC2 Antibody (A07088-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07088-1-evc2-primary-antibodies-ihc-testing-2.jpgAnti-EVC2 Antibody Picoband®IHC analysis of EVC2 using anti-EVC2 antibody (A07088-1). EVC2 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EVC2 Antibody (A07088-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07088-1-evc2-primary-antibodies-ihc-testing-3.jpgAnti-EVC2 Antibody Picoband®IHC analysis of EVC2 using anti-EVC2 antibody (A07088-1). EVC2 was detected in a paraffin-embedded section of rat midbrain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EVC2 Antibody (A07088-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07088-1-evc2-primary-antibodies-if-testing-1.jpgAnti-EVC2 Antibody Picoband®IF analysis of EVC2 using anti-EVC2 antibody (A07088-1). EVC2 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EVC2 Antibody (A07088-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1304842026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01210-3-gch1-primary-antibodies-wb-testing-1.jpgAnti-GCH1 Antibody Picoband®Western blot analysis of GCH1 using anti-GCH1 antibody (A01210-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCH1 antigen affinity purified polyclonal antibody (A01210-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GCH1 at approximately 30 kDa. The expected band size for GCH1 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01210-3-gch1-primary-antibodies-fcm-testing-1.jpgAnti-GCH1 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-GCH1 antibody (A01210-3). Overlay histogram showing Jurkat cells stained with A01210-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GCH1 Antibody (A01210-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304852026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09444-1-fgd5-primary-antibodies-wb-testing-1.jpgAnti-FGD5 Antibody Picoband®Western blot analysis of FGD5 using anti-FGD5 antibody (A09444-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat ovary tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGD5 antigen affinity purified polyclonal antibody (A09444-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FGD5 at approximately 65 kDa. The expected band size for FGD5 is at 160 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09444-1-fgd5-primary-antibodies-fcm-testing-1.jpgAnti-FGD5 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-FGD5 antibody (A09444-1). Overlay histogram showing 293T cells stained with A09444-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FGD5 Antibody (A09444-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304862026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06488-2-ggct-primary-antibodies-wb-testing-1.jpgAnti-GGCT Antibody Picoband®Western blot analysis of GGCT using anti-GGCT antibody (A06488-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGCT antigen affinity purified polyclonal antibody (A06488-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GGCT at approximately 21 kDa. The expected band size for GGCT is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06488-2-ggct-primary-antibodies-fcm-testing-1.jpgAnti-GGCT Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-GGCT antibody (A06488-2). Overlay histogram showing RT4 cells stained with A06488-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GGCT Antibody (A06488-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304872026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00355-1-nav1-primary-antibodies-wb-testing-1.jpgAnti-NAV1 Antibody Picoband®Western blot analysis of NAV1 using anti-NAV1 antibody (A00355-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAV1 antigen affinity purified polyclonal antibody (A00355-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NAV1 at approximately 202 kDa. The expected band size for NAV1 is at 202 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00355-1-nav1-primary-antibodies-ihc-testing-1.jpgAnti-NAV1 Antibody Picoband®IHC analysis of NAV1 using anti-NAV1 antibody (A00355-1). NAV1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NAV1 Antibody (A00355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00355-1-nav1-primary-antibodies-ihc-testing-2.jpgAnti-NAV1 Antibody Picoband®IHC analysis of NAV1 using anti-NAV1 antibody (A00355-1). NAV1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NAV1 Antibody (A00355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00355-1-nav1-primary-antibodies-ihc-testing-3.jpgAnti-NAV1 Antibody Picoband®IHC analysis of NAV1 using anti-NAV1 antibody (A00355-1). NAV1 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NAV1 Antibody (A00355-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00355-1-nav1-primary-antibodies-if-testing-1.jpgAnti-NAV1 Antibody Picoband®IF analysis of NAV1 using anti-NAV1 antibody (A00355-1). NAV1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NAV1 Antibody (A00355-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1304882026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03176-1-pdzk1-primary-antibodies-wb-testing-1_1.jpgAnti-PDZK1 Antibody Picoband®Western blot analysis of PDZK1 using anti-PDZK1 antibody (A03176-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse liver tissue lysates. Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDZK1 antigen affinity purified polyclonal antibody (A03176-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDZK1 at approximately 70 kDa. The expected band size for PDZK1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03176-1-pdzk1-primary-antibodies-ihc-testing-1.jpgAnti-PDZK1 Antibody Picoband®IHC analysis of PDZK1 using anti-PDZK1 antibody (A03176-1). PDZK1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDZK1 Antibody (A03176-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03176-1-pdzk1-primary-antibodies-fcm-testing-1_1.jpgAnti-PDZK1 Antibody Picoband®Flow Cytometry analysis of RM-1 cells using anti-PDZK1 antibody (A03176-1). Overlay histogram showing RM-1 cells stained with A03176-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PDZK1 Antibody (A03176-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03176-1-pdzk1-primary-antibodies-fcm-testing-2.jpgAnti-PDZK1 Antibody Picoband®Flow Cytometry analysis of CACO-2 cells using anti-PDZK1 antibody (A03176-1). Overlay histogram showing CACO-2 cells stained with A03176-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PDZK1 Antibody (A03176-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304892026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08135-pgs1-primary-antibodies-wb-testing-1.jpgAnti-PGS1 Antibody Picoband®Western blot analysis of PGS1 using anti-PGS1 antibody (A08135). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGS1 antigen affinity purified polyclonal antibody (A08135) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PGS1 at approximately 63 kDa. The expected band size for PGS1 is at 63 kDa. https://www.bosterbio.com/catalog/product/view/id/1304902026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10427-1-pdzd8-primary-antibodies-wb-testing-1.jpgAnti-PDZD8 Antibody Picoband®Western blot analysis of PDZD8 using anti-PDZD8 antibody (A10427-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDZD8 antigen affinity purified polyclonal antibody (A10427-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDZD8 at approximately 150 kDa. The expected band size for PDZD8 is at 129 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10427-1-pdzd8-primary-antibodies-ihc-testing-1.jpgAnti-PDZD8 Antibody Picoband®IHC analysis of PDZD8 using anti-PDZD8 antibody (A10427-1). PDZD8 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDZD8 Antibody (A10427-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10427-1-pdzd8-primary-antibodies-ihc-testing-2.jpgAnti-PDZD8 Antibody Picoband®IHC analysis of PDZD8 using anti-PDZD8 antibody (A10427-1). PDZD8 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDZD8 Antibody (A10427-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10427-1-pdzd8-primary-antibodies-ihc-testing-3.jpgAnti-PDZD8 Antibody Picoband®IHC analysis of PDZD8 using anti-PDZD8 antibody (A10427-1). PDZD8 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDZD8 Antibody (A10427-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10427-1-pdzd8-primary-antibodies-if-testing-1.jpgAnti-PDZD8 Antibody Picoband®IF analysis of PDZD8 using anti-PDZD8 antibody (A10427-1). PDZD8 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PDZD8 Antibody (A10427-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10427-1-pdzd8-primary-antibodies-fcm-testing-1.jpgAnti-PDZD8 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-PDZD8 antibody (A10427-1). Overlay histogram showing A549 cells stained with A10427-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDZD8 Antibody (A10427-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304912026-03-17T05:17:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03025-2-pex1-primary-antibodies-wb-testing-1.jpgAnti-PEX1 Antibody Picoband®Western blot analysis of PEX1 using anti-PEX1 antibody (A03025-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse RenCa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEX1 antigen affinity purified polyclonal antibody (A03025-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PEX1 at approximately 143 kDa. The expected band size for PEX1 is at 143 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03025-2-pex1-primary-antibodies-fcm-testing-1.jpgAnti-PEX1 Antibody Picoband®Flow Cytometry analysis of Neuro-2a cells using anti-PEX1 antibody (A03025-2). Overlay histogram showing Neuro-2a cells stained with A03025-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PEX1 Antibody (A03025-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304922026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03288-2-phf8-primary-antibodies-wb-testing-1.jpgAnti-PHF8 Antibody Picoband®Western blot analysis of PHF8 using anti-PHF8 antibody (A03288-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF8 antigen affinity purified polyclonal antibody (A03288-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHF8 at approximately 140 kDa. The expected band size for PHF8 is at 118 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03288-2-phf8-primary-antibodies-if-testing-1.jpgAnti-PHF8 Antibody Picoband®IF analysis of PHF8 using anti-PHF8 antibody (A03288-2) and anti-Beta Tubulin antibody (M01857-3). PHF8 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PHF8 Antibody (A03288-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03288-2-phf8-primary-antibodies-fcm-testing-1.jpgAnti-PHF8 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-PHF8 antibody (A03288-2). Overlay histogram showing Jurkat cells stained with A03288-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHF8 Antibody (A03288-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304932026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02315-1-pif1-primary-antibodies-wb-testing-1.jpgAnti-PIF1 Antibody Picoband®Western blot analysis of PIF1 using anti-PIF1 antibody (A02315-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIF1 antigen affinity purified polyclonal antibody (A02315-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIF1 at approximately 77 kDa. The expected band size for PIF1 is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02315-1-pif1-primary-antibodies-ihc-testing-1.jpgAnti-PIF1 Antibody Picoband®IHC analysis of PIF1 using anti-PIF1 antibody (A02315-1). PIF1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIF1 Antibody (A02315-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02315-1-pif1-primary-antibodies-fcm-testing-1.jpgAnti-PIF1 Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-PIF1 antibody (A02315-1). Overlay histogram showing HeLa cells stained with A02315-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIF1 Antibody (A02315-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304942026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14448-1-piwil3-primary-antibodies-wb-testing-1.jpgAnti-PIWIL3 Antibody Picoband®Western blot analysis of PIWIL3 using anti-PIWIL3 antibody (A14448-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIWIL3 antigen affinity purified polyclonal antibody (A14448-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIWIL3 at approximately 101 kDa. The expected band size for PIWIL3 is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14448-1-piwil3-primary-antibodies-ihc-testing-1.jpgAnti-PIWIL3 Antibody Picoband®IHC analysis of PIWIL3 using anti-PIWIL3 antibody (A14448-1). PIWIL3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIWIL3 Antibody (A14448-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14448-1-piwil3-primary-antibodies-ihc-testing-2.jpgAnti-PIWIL3 Antibody Picoband®IHC analysis of PIWIL3 using anti-PIWIL3 antibody (A14448-1). PIWIL3 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIWIL3 Antibody (A14448-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1304952026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05094-1-plcd1-primary-antibodies-wb-testing-1.jpgAnti-PLCD1 Antibody Picoband®Western blot analysis of PLCD1 using anti-PLCD1 antibody (A05094-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLCD1 antigen affinity purified polyclonal antibody (A05094-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLCD1 at approximately 86 kDa. The expected band size for PLCD1 is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05094-1-plcd1-primary-antibodies-fcm-testing-1.jpgAnti-PLCD1 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-PLCD1 antibody (A05094-1). Overlay histogram showing 293T cells stained with A05094-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLCD1 Antibody (A05094-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304962026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05479-1-plin5-primary-antibodies-wb-testing-1.jpgAnti-PLIN5 Antibody Picoband®Western blot analysis of PLIN5 using anti-PLIN5 antibody (A05479-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HT1080 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLIN5 antigen affinity purified polyclonal antibody (A05479-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLIN5 at approximately 55 kDa. The expected band size for PLIN5 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05479-1-plin5-primary-antibodies-ihc-testing-1.jpgAnti-PLIN5 Antibody Picoband®IHC analysis of PLIN5 using anti-PLIN5 antibody (A05479-1). PLIN5 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLIN5 Antibody (A05479-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05479-1-plin5-primary-antibodies-ihc-testing-2.jpgAnti-PLIN5 Antibody Picoband®IHC analysis of PLIN5 using anti-PLIN5 antibody (A05479-1). PLIN5 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLIN5 Antibody (A05479-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05479-1-plin5-primary-antibodies-ihc-testing-3.jpgAnti-PLIN5 Antibody Picoband®IHC analysis of PLIN5 using anti-PLIN5 antibody (A05479-1). PLIN5 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLIN5 Antibody (A05479-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05479-1-plin5-primary-antibodies-ihc-testing-4.jpgAnti-PLIN5 Antibody Picoband®IHC analysis of PLIN5 using anti-PLIN5 antibody (A05479-1). PLIN5 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLIN5 Antibody (A05479-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05479-1-plin5-primary-antibodies-if-testing-1.jpgAnti-PLIN5 Antibody Picoband®IF analysis of PLIN5 using anti-PLIN5 antibody (A05479-1). PLIN5 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PLIN5 Antibody (A05479-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05479-1-plin5-primary-antibodies-if-testing-2.jpgAnti-PLIN5 Antibody Picoband®IF analysis of PLIN5 using anti-PLIN5 antibody (A05479-1). PLIN5 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PLIN5 Antibody (A05479-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05479-1-plin5-primary-antibodies-fcm-testing-1.jpgAnti-PLIN5 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-PLIN5 antibody (A05479-1). Overlay histogram showing HepG2 cells stained with A05479-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLIN5 Antibody (A05479-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1304972026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08812-2-polm-primary-antibodies-wb-testing-1.jpgAnti-POLM Antibody Picoband®Western blot analysis of POLM using anti-POLM antibody (A08812-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLM antigen affinity purified polyclonal antibody (A08812-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POLM at approximately 55 kDa. The expected band size for POLM is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08812-2-polm-primary-antibodies-ip-testing-1.jpgAnti-POLM Antibody Picoband®Immunoprecipitating POLM in 293T whole cell lysate. Western blot analysis of POLM using anti-POLM antibody (A08812-2). Lane 1: 293T whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-POLM antibody in 293T whole cell lysate, Lane 3: anti-POLM antibody (2μg) + 293T whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-POLM antigen affinity purified polyclonal antibody (A08812-2) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for POLM at approximately 55 kDa. The expected band size for POLM is at 55 kDa. https://www.bosterbio.com/catalog/product/view/id/1304982026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03566-2-pnkp-primary-antibodies-wb-testing-1.jpgAnti-PNKP Antibody Picoband®Western blot analysis of PNKP using anti-PNKP antibody (A03566-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNKP antigen affinity purified polyclonal antibody (A03566-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PNKP at approximately 57 kDa. The expected band size for PNKP is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03566-2-pnkp-primary-antibodies-if-testing-1.jpgAnti-PNKP Antibody Picoband®IF analysis of PNKP using anti-PNKP antibody (A03566-2) and anti-Beta Tubulin antibody (M01857-3). PNKP was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PNKP Antibody (A03566-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03566-2-pnkp-primary-antibodies-fcm-testing-1.jpgAnti-PNKP Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-PNKP antibody (A03566-2). Overlay histogram showing U251 cells stained with A03566-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNKP Antibody (A03566-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p53-rabbit-monoclonal-antibody-m00001-7-boster.html2026-03-16T05:08:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00001-7-tp53-primary-antibodies-ihc-testing-1.jpgAnti-P53 Rabbit Monoclonal AntibodyHuman ovarian serous carcinoma tissue was stained with anti-P53 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00001-7-tp53-primary-antibodies-wb-testing-1.jpgAnti-P53 Rabbit Monoclonal AntibodyWhole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-P53 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1:HEK293 Predicted band size: 53kDa Observed band size: 53kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tnf-alpha-rabbit-monoclonal-antibody-m00002-16-boster.html2026-03-16T05:08:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00002-16-tnf-primary-antibodies-ihc-testing-1.jpgAnti-TNF α Rabbit Monoclonal AntibodyMouse pancreas was stained with anti-TNF α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00002-16-tnf-primary-antibodies-ihc-testing-2.jpgAnti-TNF α Rabbit Monoclonal AntibodyRat pancreas was stained with anti-TNF α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00002-16-tnf-primary-antibodies-wb-testing-1.jpgAnti-TNF α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TNF α antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: THP-1 tearted with Phorbol 12-myristate 13-acetate and Lipopolysaccharide (100 mg/mL) for 24 hours Lane 2: RAW264.7 tearted with Lipopolysaccharide (100 mg/mL) for 24 hours Predicted band size: 26,18kDa Observed band size: 26,18kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mtor-rabbit-monoclonal-antibody-m00003-4-boster.html2026-03-16T05:08:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00003-4-mtor-primary-antibodies-ihc-testing-1.jpgAnti-mTOR Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-mTOR rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00003-4-mtor-primary-antibodies-ihc-testing-2.jpgAnti-mTOR Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-mTOR rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00003-4-mtor-primary-antibodies-wb-testing-1.jpgAnti-mTOR Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-mTOR antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C2C12 Lane 4: C6 Predicted band size: 289kDa Observed band size: 260kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mtor-rabbit-monoclonal-antibody-m00003-5-boster.html2026-03-16T05:08:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00003-5-mtor-primary-antibodies-ihc-testing-1.jpgAnti-mTOR Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-mTOR (phospho Ser2448) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00003-5-mtor-primary-antibodies-ihc-testing-2.jpgAnti-mTOR Rabbit Monoclonal AntibodyHuman endometrium carcinoma was stained with anti-mTOR (phospho Ser2448) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00003-5-mtor-primary-antibodies-ihc-testing-3.jpgAnti-mTOR Rabbit Monoclonal AntibodyMouse colon was stained with anti-mTOR (phospho Ser2448) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00003-5-mtor-primary-antibodies-ihc-testing-4.jpgAnti-mTOR Rabbit Monoclonal AntibodyRat colon was stained with anti-mTOR (phospho Ser2448) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00003-5-mtor-primary-antibodies-wb-testing-1.jpgAnti-mTOR Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-mTOR (phospho Ser2448) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: MCF7 Lane 3: NIH-3T3 Predicted band size: 289kDa Observed band size: 250kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-beta-catenin-rabbit-monoclonal-antibody-m00004-7-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00004-7-ctnnb1-primary-antibodies-ihc-testing-1.jpgAnti-β Catenin Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-β Catenin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00004-7-ctnnb1-primary-antibodies-ihc-testing-2.jpgAnti-β Catenin Rabbit Monoclonal AntibodyMouse stomach was stained with anti-β Catenin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00004-7-ctnnb1-primary-antibodies-ihc-testing-3.jpgAnti-β Catenin Rabbit Monoclonal AntibodyRat stomach was stained with anti-β Catenin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00004-7-ctnnb1-primary-antibodies-wb-testing-1.jpgAnti-β Catenin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-β Catenin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HCT116 Lane 2: HeLa Lane 3: C6 Lane 4: U14 Predicted band size: 84kDa Observed band size: 96kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-beta-catenin-rabbit-monoclonal-antibody-m00004-8-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00004-8-ctnnb1-primary-antibodies-wb-testing-1.jpgAnti-β Catenin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-β Catenin (Phospho Thr41/Ser45) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SW620 Lane 2: SW480 Predicted band size: 86kDa Observed band size: 92kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pten-rabbit-monoclonal-antibody-m00006-5-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00006-5-pten-primary-antibodies-ihc-testing-1.jpgAnti-PTEN Rabbit Monoclonal AntibodyHuman panreas was stained with anti-PTEN rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00006-5-pten-primary-antibodies-ihc-testing-2.jpgAnti-PTEN Rabbit Monoclonal AntibodyRat panreas was stained with anti-PTEN rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00006-5-pten-primary-antibodies-wb-testing-1.jpgAnti-PTEN Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PTEN antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MCF7 Lane 2: Mouse testis Lane 3: Rat testis Lane 4: HeLa Predicted band size: 47kDa Observed band size: 56kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stat3-rabbit-monoclonal-antibody-m00007-4-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00007-4-stat3-primary-antibodies-ihc-testing-1.jpgAnti-STAT3 Rabbit Monoclonal AntibodyHuman pancreas was stained with anti-STAT3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00007-4-stat3-primary-antibodies-wb-testing-1.jpgAnti-STAT3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-STAT3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MCF7 Lane 2: HeLa Lane 3: RAW264.7 Predicted band size: 88kDa Observed band size: 88kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-her2-rabbit-monoclonal-antibody-m00010-21-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00010-21-erbb2-primary-antibodies-ihc-testing-1.jpgAnti-HER2 Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-HER2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00010-21-erbb2-primary-antibodies-wb-testing-1.jpgAnti-HER2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HER2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SK-BR-3 Lane 2: C6 Lane 3: T47D Predicted band size: 138kDa Observed band size: 185kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-foxp3-rabbit-monoclonal-antibody-m00011-5-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00011-5-foxp3-primary-antibodies-ihc-testing-1.jpgAnti-FOXP3 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-FOXP3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00011-5-foxp3-primary-antibodies-ihc-testing-2.jpgAnti-FOXP3 Rabbit Monoclonal AntibodyRat spleen was stained with anti-FOXP3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00011-5-foxp3-primary-antibodies-ihc-testing-3.jpgAnti-FOXP3 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-FOXP3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00011-5-foxp3-primary-antibodies-wb-testing-1.jpgAnti-FOXP3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FOXP3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-14 Lane 2: 4T1 Lane 3: Rat skin Lane 4: Mouse skin Lane 5: HeLa Predicted band size: 47kDa Observed band size: 47kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cdkn2a-p14arf-rabbit-monoclonal-antibody-m00016-3-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00016-3-cdkn2a-primary-antibodies-wb-testing-1.jpgAnti-CDKN2A/p14ARF Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CDKN2A/p14ARF antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: PC-3 Predicted band size: 14kDa Observed band size: 16kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p16-ink4a-rabbit-monoclonal-antibody-m00016-4-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00016-4-cdkn2a-primary-antibodies-wb-testing-1.jpgAnti-p16 INK4A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p16 INK4A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HeLa Predicted band size: 17kDa Observed band size: 17kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sirt1-rabbit-monoclonal-antibody-m00018-2-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00018-2-sirt1-primary-antibodies-ihc-testing-1.jpgAnti-SIRT1 Rabbit Monoclonal AntibodyMouse testis was stained with Anti-SIRT1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00018-2-sirt1-primary-antibodies-ihc-testing-2.jpgAnti-SIRT1 Rabbit Monoclonal AntibodyHuman testis was stained with Anti-SIRT1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00018-2-sirt1-primary-antibodies-wb-testing-1.jpgAnti-SIRT1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SIRT1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Jurkat Predicted band size: 81kDa Observed band size: 130kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tgf-beta1-3-rabbit-monoclonal-antibody-m00019-11-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00019-11-tgfb1-primary-antibodies-ihc-testing-1.jpgAnti-TGF β1/3 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-TGF β1/3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00019-11-tgfb1-primary-antibodies-ihc-testing-2.jpgAnti-TGF β1/3 Rabbit Monoclonal AntibodyRat spleen was stained with anti-TGF β1/3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00019-11-tgfb1-primary-antibodies-wb-testing-1.jpgAnti-TGF β1/3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TGF β1/3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: MCF7 Lane 3: Mouse spleen Lane 4: Rat spleen Predicted band size: 44kDa Observed band size: 44,13kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tgf-beta1-rabbit-monoclonal-antibody-m00019-12-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00019-12-tgfb1-primary-antibodies-ihc-testing-1.jpgAnti-TGF β1 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-TGF β1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00019-12-tgfb1-primary-antibodies-ihc-testing-2.jpgAnti-TGF β1 Rabbit Monoclonal AntibodyRat spleen was stained with anti-TGF β1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00019-12-tgfb1-primary-antibodies-wb-testing-1.jpgAnti-TGF β1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TGF β1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: K562 Lane 3: Mouse spleen Lane 4: Rat spleen Predicted band size: 44kDa Observed band size: 44,13kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il-10-rabbit-monoclonal-antibody-m00021-3-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00021-3-il10-primary-antibodies-wb-testing-1.jpgAnti-IL-10 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IL-10 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HL-60 Lane 2: THP-1 Predicted band size: 21kDa Observed band size: 21kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-egfr-rabbit-monoclonal-antibody-m00023-30-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-30-egfr-primary-antibodies-ihc-testing-1.jpgAnti-EGFR Rabbit Monoclonal AntibodyMouse skin was stained with anti-EGFR rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-30-egfr-primary-antibodies-ihc-testing-2.jpgAnti-EGFR Rabbit Monoclonal AntibodyHuman cutaneous squamous cell carcinoma was stained with anti-EGFR rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00023-30-egfr-primary-antibodies-wb-testing-1.jpgAnti-EGFR Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-EGFR antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Rat womb Predicted band size: 134kDa Observed band size: 175kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-akt-rabbit-monoclonal-antibody-m00024-8-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-8-akt-primary-antibodies-ihc-testing-1.jpgAnti-AKT Rabbit Monoclonal AntibodyMouse lung was stained with anti-AKT (Phospho Ser473) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-8-akt-primary-antibodies-ihc-testing-2.jpgAnti-AKT Rabbit Monoclonal AntibodyRat lung was stained with anti-AKT (Phospho Ser473) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-8-akt-primary-antibodies-ihc-testing-3.jpgAnti-AKT Rabbit Monoclonal AntibodyHuman lung carcinoma was stained with anti-AKT (Phospho Ser473) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-8-akt-primary-antibodies-wb-testing-1.jpgAnti-AKT Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-AKT (Phospho Ser473) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: PC-12 Predicted band size: 55kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-akt-rabbit-monoclonal-antibody-m00024-9-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-9-akt1-primary-antibodies-wb-testing-1.jpgAnti-AKT Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-AKT antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Jurkat Lane 3: C6 Lane 4: Mouse brain Predicted band size: 55kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c-myc-rabbit-monoclonal-antibody-m00026-6-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00026-6-myc-primary-antibodies-ihc-testing-1.jpgAnti-c-Myc Rabbit Monoclonal AntibodyHuman tonsil was stained with Anti-c-Myc rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00026-6-myc-primary-antibodies-ihc-testing-2.jpgAnti-c-Myc Rabbit Monoclonal AntibodyHuman lung was stained with Anti-c-Myc rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00026-6-myc-primary-antibodies-wb-testing-1.jpgAnti-c-Myc Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-c-Myc antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A172 Lane 2: MCF7 Predicted band size: 48kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-jak2-rabbit-monoclonal-antibody-m00027-3-boster.html2026-03-16T05:09:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00027-3-jak2-primary-antibodies-ihc-testing-1.jpgAnti-JAK2 Rabbit Monoclonal AntibodyHuman lung carcinoma was stained with anti-JAK2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00027-3-jak2-primary-antibodies-ihc-testing-2.jpgAnti-JAK2 Rabbit Monoclonal AntibodyMouse lung was stained with anti-JAK2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00027-3-jak2-primary-antibodies-ihc-testing-3.jpgAnti-JAK2 Rabbit Monoclonal AntibodyRat lung was stained with anti-JAK2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00027-3-jak2-primary-antibodies-wb-testing-1.jpgAnti-JAK2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-JAK2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Predicted band size: 131kDa Observed band size: 131kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-erk1-2-rabbit-monoclonal-antibody-m00030-2-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00030-2-mapk1-primary-antibodies-ihc-testing-1.jpgAnti-ERK1/2 Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-ERK1/2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00030-2-mapk1-primary-antibodies-wb-testing-1.jpgAnti-ERK1/2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ERK1/2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: HeLa Lane 3: C6 Lane 4: K562 Predicted band size: 43,41kDa Observed band size: 43,41kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-erk-1-2-rabbit-monoclonal-antibody-m00030-3-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00030-3-mapk1-primary-antibodies-wb-testing-1.jpgAnti-Erk 1/2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Erk1/2(phospho Thr202/Tyr204) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: HeLa was starved of serum for 24 hours and then treated with 20% fetal bovine serum for 15 minutes, followed by Calyculin A treatment for 15 minutes Predicted band size: 44,42kDa Observed band size: 44,42kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-notch1-rabbit-monoclonal-antibody-m00033-4-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00033-4-notch1-primary-antibodies-wb-testing-1.jpgAnti-Notch1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 6% SDS-PAGE, and the membrane was blotted with anti-Notch1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: C6 Lane 3: Mouse spleen Predicted band size: 273kDa Observed band size: 120kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nlrp3-rabbit-monoclonal-antibody-m00034-1-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00034-1-nlrp3-primary-antibodies-ihc-testing-1.jpgAnti-NLRP3 Rabbit Monoclonal AntibodyRat kidney tissue was stained with Anti-NLRP3 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00034-1-nlrp3-primary-antibodies-wb-testing-1.jpgAnti-NLRP3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NLRP3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: J774A.1 Lane 2: RAW364.7 Lane 3: THP-1 Predicted band size: 118kDa Observed band size: 118kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stat1-alpha-rabbit-monoclonal-antibody-m00036-6-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-6-stat1-primary-antibodies-ihc-testing-1.jpgAnti-STAT1 α Rabbit Monoclonal AntibodyRat colon was stained with Anti-STAT1 α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-6-stat1-primary-antibodies-ihc-testing-2.jpgAnti-STAT1 α Rabbit Monoclonal AntibodyHuman kidney was stained with Anti-STAT1 α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-6-stat1-primary-antibodies-ihc-testing-3.jpgAnti-STAT1 α Rabbit Monoclonal AntibodyMouse colon was stained with Anti-STAT1 α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00036-6-stat1-primary-antibodies-wb-testing-1.jpgAnti-STAT1 α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-STAT1 α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: PC12 Lane 3: NIH3T3 Predicted band size: 87kDa Observed band size: 90kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bcl-2-rabbit-monoclonal-antibody-m00040-4-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00040-4-bcl2-primary-antibodies-ihc-testing-1.jpgAnti-Bcl-2 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-Bcl-2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00040-4-bcl2-primary-antibodies-ihc-testing-2.jpgAnti-Bcl-2 Rabbit Monoclonal AntibodyRat spleen was stained with anti-Bcl-2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00040-4-bcl2-primary-antibodies-wb-testing-1.jpgAnti-Bcl-2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Bcl-2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: K562 Lane 3: 3T3-L1 Lane 4: Rat spleen Predicted band size: 26kDa Observed band size: 26kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vegfa-rabbit-monoclonal-antibody-m00045-8-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00045-8-vegfa-primary-antibodies-ihc-testing-1.jpgAnti-VEGFA Rabbit Monoclonal AntibodyRat brain was stained with anti-VEGFA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00045-8-vegfa-primary-antibodies-ihc-testing-2.jpgAnti-VEGFA Rabbit Monoclonal AntibodyHuman kidney was stained with anti-VEGFA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00045-8-vegfa-primary-antibodies-ihc-testing-3.jpgAnti-VEGFA Rabbit Monoclonal AntibodyMouse brain was stained with anti-VEGFA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00045-8-vegfa-primary-antibodies-wb-testing-1.jpgAnti-VEGFA Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-VEGFA antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: U-87 MG Lane 3: C6 Lane 4: Neuro-2a Predicted band size: 27kDa Observed band size: 40kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-caspase-1-rabbit-monoclonal-antibody-m00048-3-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00048-3-casp1-primary-antibodies-ihc-testing-1.jpgAnti-Caspase-1 Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-Caspase-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00048-3-casp1-primary-antibodies-ihc-testing-2.jpgAnti-Caspase-1 Rabbit Monoclonal AntibodyHuman small intestine was stained with anti-Caspase-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00048-3-casp1-primary-antibodies-wb-testing-1.jpgAnti-Caspase-1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Caspase-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: THP-1 Predicted band size: 45kDa Observed band size: 45kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ezh2-rabbit-monoclonal-antibody-m00050-2-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00050-2-ezh2-primary-antibodies-ihc-testing-1.jpgAnti-EZH2 Rabbit Monoclonal AntibodyHumantonsil was stained with anti-EZH2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00050-2-ezh2-primary-antibodies-wb-testing-1.jpgAnti-EZH2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-EZH2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: 3T3-L1 Predicted band size: 85kDa Observed band size: 85kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd44-rabbit-monoclonal-antibody-m00052-7-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00052-7-cd44-primary-antibodies-ihc-testing-1.jpgAnti-CD44 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-CD44 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00052-7-cd44-primary-antibodies-ihc-testing-2.jpgAnti-CD44 Rabbit Monoclonal AntibodyRat liver was stained with anti-CD44 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00052-7-cd44-primary-antibodies-ihc-testing-3.jpgAnti-CD44 Rabbit Monoclonal AntibodyHuman liver was stained with anti-CD44 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00052-7-cd44-primary-antibodies-ihc-testing-4.jpgAnti-CD44 Rabbit Monoclonal AntibodyMouse liver was stained with anti-CD44 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00052-7-cd44-primary-antibodies-wb-testing-1.jpgAnti-CD44 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD44 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: U-14 Lane 3: Rat lung Predicted band size: 81kDa Observed band size: 81kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mdm2-rabbit-monoclonal-antibody-m00054-3-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00054-3-mdm2-primary-antibodies-ihc-testing-1.jpgAnti-MDM2 Rabbit Monoclonal AntibodyRat lung was stained with anti-MDM2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00054-3-mdm2-primary-antibodies-ihc-testing-2.jpgAnti-MDM2 Rabbit Monoclonal AntibodyHuman placenta was stained with anti-MDM2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00054-3-mdm2-primary-antibodies-wb-testing-1.jpgAnti-MDM2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MDM2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: C2C12 Lane 3: Raji Predicted band size: 55kDa Observed band size: 90kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fas-rabbit-monoclonal-antibody-m00055-3-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00055-3-fas-primary-antibodies-ihc-testing-1.jpgAnti-Fas Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Fas rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00055-3-fas-primary-antibodies-wb-testing-1.jpgAnti-Fas Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Fas antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Predicted band size: 38kDa Observed band size: 40kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-estrogen-receptor-alpha-rabbit-monoclonal-antibody-m00057-5-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00057-5-esr1-primary-antibodies-ihc-testing-1.jpgAnti-Estrogen Receptor α Rabbit Monoclonal AntibodyHuman ovary carcinoma was stained with anti-Estrogen Receptor α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00057-5-esr1-primary-antibodies-ihc-testing-2.jpgAnti-Estrogen Receptor α Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-Estrogen Receptor α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00057-5-esr1-primary-antibodies-wb-testing-1.jpgAnti-Estrogen Receptor α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti- Estrogen Receptor α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MCF7 Lane 2: SKOV3 Lane 3: 4T1 Predicted band size: 66kDa Observed band size: 66kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hmgb1-rabbit-monoclonal-antibody-m00066-6-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00066-6-hmgb1-primary-antibodies-ihc-testing-1.jpgAnti-HMGB1 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-HMGB1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00066-6-hmgb1-primary-antibodies-wb-testing-1.jpgAnti-HMGB1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HMGB1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: K562 Lane 3: C6 Lane 4: 3T3-L1 Predicted band size: 25kDa Observed band size: 25kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-igf1-receptor-rabbit-monoclonal-antibody-m00070-12-boster.html2026-03-16T05:09:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00070-12-igf1r-primary-antibodies-ihc-testing-1.jpgAnti-IGF1 Receptor Rabbit Monoclonal AntibodyHuman bladder carcinoma was stained with Anti-IGF1 Receptor rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00070-12-igf1r-primary-antibodies-wb-testing-1.jpgAnti-IGF1 Receptor Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IGF1 Receptor antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: LoVo Lane 2: MCF7 Predicted band size: 154kDa Observed band size: 200kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-foxo1a-rabbit-monoclonal-antibody-m00073-3-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00073-3-foxo1-primary-antibodies-wb-testing-1.jpgAnti-FOXO1A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FOXO1A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: HePa1-6 Predicted band size: 75kDa Observed band size: 75kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smad4-rabbit-monoclonal-antibody-m00074-2-boster.html2026-04-06T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00074-2-smad4-primary-antibodies-ihc-testing-1.jpgAnti-Smad4 Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-Smad4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00074-2-smad4-primary-antibodies-ihc-testing-2.jpgAnti-Smad4 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Smad4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00074-2-smad4-primary-antibodies-wb-testing-1.jpgAnti-Smad4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Smad4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: HepG2 Lane 3: NIH-3T3 Lane 4: Ramos Predicted band size: 60kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-braf-rabbit-monoclonal-antibody-m00075-6-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00075-6-braf-primary-antibodies-ihc-testing-1.jpgAnti-BRAF Rabbit Monoclonal AntibodyHuman testis was stained with anti-Raf-B (phospho Thr401) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00075-6-braf-primary-antibodies-wb-testing-1.jpgAnti-BRAF Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Raf-B (phospho Thr401) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: HeLa was starved of serum for 24 hours and then treated with 20% fetal bovine serum for 15 minutes, followed by Calyculin A treatment for 15 minutes Predicted band size: 84kDa Observed band size: 84kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-caspase-9-rabbit-monoclonal-antibody-m00080-6-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00080-6-casp9-primary-antibodies-wb-testing-1.jpgAnti-Caspase-9 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Caspase-9 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: THP-1 Predicted band size: 46kDa Observed band size: 40kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-amyloid-beta-rabbit-monoclonal-antibody-m00081-9-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00081-9-app-primary-antibodies-ihc-testing-1.jpgAnti-Amyloid-β Rabbit Monoclonal AntibodyHuman brain was stained with anti-Amyloid-β rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00081-9-app-primary-antibodies-ihc-testing-2.jpgAnti-Amyloid-β Rabbit Monoclonal AntibodyHuman gliocytoma was stained with anti-Amyloid-β rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00081-9-app-primary-antibodies-ihc-testing-3.jpgAnti-Amyloid-β Rabbit Monoclonal AntibodyMouse brain was stained with anti-Amyloid-β rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00081-9-app-primary-antibodies-ihc-testing-4.jpgAnti-Amyloid-β Rabbit Monoclonal AntibodyRat brain was stained with anti-Amyloid-β rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00081-9-app-primary-antibodies-wb-testing-1.jpgAnti-Amyloid-β Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Amyloid-β antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: Mouse brain Lane 3: HeLa Predicted band size: 87kDa Observed band size: 100kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cox2-rabbit-monoclonal-antibody-m00084-6-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00084-6-ptgs2-primary-antibodies-ihc-testing-1.jpgAnti-COX2 Rabbit Monoclonal AntibodyHuman appendix was stained with anti-COX2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00084-6-ptgs2-primary-antibodies-wb-testing-1.jpgAnti-COX2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-COX2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: RAW264.7 tearted with LPS(100mg/mL) of 24 hours Lane 2: A549 Lane 3: HeLa Predicted band size: 69kDa Observed band size: 75kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rad51-rabbit-monoclonal-antibody-m00088-3-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00088-3-rad51-primary-antibodies-wb-testing-1.jpgAnti-Rad51 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Rad51 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: HeLa Lane 3: NIH-3T3 Predicted band size: 37kDa Observed band size: 37kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smad2-rabbit-monoclonal-antibody-m00090-5-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00090-5-smad2-primary-antibodies-ihc-testing-1.jpgAnti-Smad2 Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-Smad2 (phospho Ser250) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00090-5-smad2-primary-antibodies-wb-testing-1.jpgAnti-Smad2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Smad2 (phospho Ser250) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 treated with TPA for 48 hours Lane 2: HeLa Lane 3: H9C2 Lane 4: RAW264.7 Predicted band size: 58kDa Observed band size: 58kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smad2-rabbit-monoclonal-antibody-m00090-6-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00090-6-smad2-primary-antibodies-wb-testing-1.jpgAnti-Smad2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Smad2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 treated with TPA of 48 hours Lane 2: HeLa Lane 3: RAW264.7 Lane 4: C6 Predicted band size: 58kDa Observed band size: 58kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pdgfr-beta-rabbit-monoclonal-antibody-m00096-6-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00096-6-pdgfrb-primary-antibodies-ihc-testing-1.jpgAnti-PDGFR-β Rabbit Monoclonal AntibodyMouse spleen was stained with anti-PDGFR-β rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00096-6-pdgfrb-primary-antibodies-ihc-testing-2.jpgAnti-PDGFR-β Rabbit Monoclonal AntibodyRat spleen was stained with anti-PDGFR-β rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00096-6-pdgfrb-primary-antibodies-ihc-testing-3.jpgAnti-PDGFR-β Rabbit Monoclonal AntibodyHuman kidney was stained with anti-PDGFR-β rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00096-6-pdgfrb-primary-antibodies-wb-testing-1.jpgAnti-PDGFR-β Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-PDGFR-β antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: NIH-3T3 Lane 3: MG-63 Predicted band size: 124kDa Observed band size: 150-190kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fgfr1-rabbit-monoclonal-antibody-m00098-5-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00098-5-fgfr1-primary-antibodies-ihc-testing-1.jpgAnti-FGFR1 Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with Anti-FGFR1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00098-5-fgfr1-primary-antibodies-wb-testing-1.jpgAnti-FGFR1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FGFR1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HepG2 Predicted band size: 91kDa Observed band size: 145kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nras-rabbit-monoclonal-antibody-m00099-4-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00099-4-nras-primary-antibodies-ihc-testing-1.jpgAnti-NRAS Rabbit Monoclonal AntibodyHuman colon was stained with anti-Ras rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00099-4-nras-primary-antibodies-wb-testing-1.jpgAnti-NRAS Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NRAS antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: C6 Lane 3: MCF7 Lane 4: HEK293 Predicted band size: 22kDa Observed band size: 22kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-src-rabbit-monoclonal-antibody-m00107-5-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00107-5-src-primary-antibodies-ihc-testing-1.jpgAnti-Src Rabbit Monoclonal AntibodyMouse kidney was stained with anti-Src rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00107-5-src-primary-antibodies-ihc-testing-2.jpgAnti-Src Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Src rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00107-5-src-primary-antibodies-wb-testing-1.jpgAnti-Src Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Src antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Mouse brain Predicted band size: 60kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sp1-rabbit-monoclonal-antibody-m00110-3-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-3-sp1-primary-antibodies-ihc-testing-1.jpgAnti-SP1 Rabbit Monoclonal AntibodyMouse colon was stained with Anti-SP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-3-sp1-primary-antibodies-ihc-testing-2.jpgAnti-SP1 Rabbit Monoclonal AntibodyRat colon was stained with Anti-SP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-3-sp1-primary-antibodies-ihc-testing-3.jpgAnti-SP1 Rabbit Monoclonal AntibodyHuman colon was stained with Anti-SP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00110-3-sp1-primary-antibodies-wb-testing-1.jpgAnti-SP1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SP1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Caco-2 Lane 2: HeLa treated with serum for 30 minutes Predicted band size: 100kDa Observed band size: 100kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hras-rabbit-monoclonal-antibody-m00114-3-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00114-3-hras-primary-antibodies-wb-testing-1.jpgAnti-HRAS Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HRAS antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-12 Lane 2: Mouse brain Lane 3: HEK293 Predicted band size: 21kDa Observed band size: 21kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-yap-rabbit-monoclonal-antibody-m00116-1-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00116-1-yap1-primary-antibodies-ihc-testing-1.jpgAnti-YAP Rabbit Monoclonal AntibodyMouse thyroid was stained with anti-YAP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00116-1-yap1-primary-antibodies-ihc-testing-2.jpgAnti-YAP Rabbit Monoclonal AntibodyRat thyroid was stained with anti-YAP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00116-1-yap1-primary-antibodies-ihc-testing-3.jpgAnti-YAP Rabbit Monoclonal AntibodyMouse skin was stained with anti-YAP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00116-1-yap1-primary-antibodies-ihc-testing-4.jpgAnti-YAP Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-YAP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00116-1-yap1-primary-antibodies-wb-testing-1.jpgAnti-YAP Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-YAP antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MCF7 Lane 2: 4T1 Lane 3: C6 Predicted band size: 55kDa Observed band size: 55-75kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-klf4-rabbit-monoclonal-antibody-m00120-3-boster.html2026-03-16T05:09:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00120-3-klf4-primary-antibodies-ihc-testing-1.jpgAnti-KLF4 Rabbit Monoclonal AntibodyMouse colon was stained with Anti-KLF4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00120-3-klf4-primary-antibodies-ihc-testing-2.jpgAnti-KLF4 Rabbit Monoclonal AntibodyRat colon was stained with Anti-KLF4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00120-3-klf4-primary-antibodies-ihc-testing-3.jpgAnti-KLF4 Rabbit Monoclonal AntibodyHuman colon was stained with Anti-KLF4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00120-3-klf4-primary-antibodies-wb-testing-1.jpgAnti-KLF4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-KLF4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HCT-116 Lane 2: LoVo Lane 3: NCCIT Lane 4: 3T3-L1 Lane 5: SMC Predicted band size: 55kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cleaved-parp1-rabbit-monoclonal-antibody-m00122-8-boster.html2026-03-16T05:09:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00122-8-parp1-primary-antibodies-ihc-testing-1.jpgAnti-Cleaved PARP1 Rabbit Monoclonal AntibodyRat colon tissue was stained with anti-Cleaved PARP1 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00122-8-parp1-primary-antibodies-ihc-testing-2.jpgAnti-Cleaved PARP1 Rabbit Monoclonal AntibodyHuman tonsil tissue was stained with anti-Cleaved PARP1 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00122-8-parp1-primary-antibodies-wb-testing-1.jpgAnti-Cleaved PARP1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cleaved PARP1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: RAW264.7 Lane 2: HeLa Predicted band size: 25kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il18-rabbit-monoclonal-antibody-m00124-4-boster.html2026-03-16T05:09:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00124-4-il18-primary-antibodies-ihc-testing-1.jpgAnti-IL18 Rabbit Monoclonal AntibodyMouse spleen was stained with Anti-IL18 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00124-4-il18-primary-antibodies-ihc-testing-2.jpgAnti-IL18 Rabbit Monoclonal AntibodyHuman tonsil was stained with Anti-IL18 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00124-4-il18-primary-antibodies-wb-testing-1.jpgAnti-IL18 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IL18 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: HeLa Predicted band size: 22kDa Observed band size: 22kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pcna-rabbit-monoclonal-antibody-m00125-5-boster.html2026-03-16T05:09:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00125-5-pcna-primary-antibodies-ihc-testing-1.jpgAnti-PCNA Rabbit Monoclonal AntibodyHuman spleen was stained with anti-PCNA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00125-5-pcna-primary-antibodies-wb-testing-1.jpgAnti-PCNA Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PCNA antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: HepG2 Lane 3: K562 Lane 4: Mouse spleen Predicted band size: 29kDa Observed band size: 36kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-parkin-rabbit-monoclonal-antibody-m00127-1-boster.html2026-03-16T05:09:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00127-1-park2-primary-antibodies-wb-testing-1.jpgAnti-Parkin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Parkin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: U-87 MG Lane 3: Neuro-2a Predicted band size: 52kDa Observed band size: 52kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ctcf-rabbit-monoclonal-antibody-m00132-3-boster.html2026-03-16T05:09:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00132-3-ctcf-primary-antibodies-ihc-testing-1.jpgAnti-CTCF Rabbit Monoclonal AntibodyMouse kidney was stained with anti-CTCF rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00132-3-ctcf-primary-antibodies-ihc-testing-2.jpgAnti-CTCF Rabbit Monoclonal AntibodyRat kidney was stained with anti-CTCF rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00132-3-ctcf-primary-antibodies-wb-testing-1.jpgAnti-CTCF Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CTCF antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: U-14 Predicted band size: 83kDa Observed band size: 140kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-abl1-rabbit-monoclonal-antibody-m00133-1-boster.html2026-03-16T05:09:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00133-1-abl1-primary-antibodies-wb-testing-1.jpgAnti-ABL1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-ABL1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Predicted band size: 123kDa Observed band size: 123kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd14-rabbit-monoclonal-antibody-m00137-4-boster.html2026-03-16T05:09:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00137-4-cd14-primary-antibodies-ihc-testing-1.jpgAnti-CD14 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-CD14 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00137-4-cd14-primary-antibodies-ihc-testing-2.jpgAnti-CD14 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-CD14 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00137-4-cd14-primary-antibodies-wb-testing-1.jpgAnti-CD14 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD14 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: THP-1 Lane 2: SW480 Lane 3: Rat thymus Predicted band size: 40kDa Observed band size: 50kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd14-rabbit-monoclonal-antibody-m00137-5-boster.html2026-03-16T05:09:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00137-5-cd14-primary-antibodies-ihc-testing-1.jpgAnti-CD14 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-CD14 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00137-5-cd14-primary-antibodies-ihc-testing-2.jpgAnti-CD14 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-CD14 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00137-5-cd14-primary-antibodies-wb-testing-1.jpgAnti-CD14 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD14 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: RAW264.7 Predicted band size: 39kDa Observed band size: 52kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mmp9-rabbit-monoclonal-antibody-m00139-4-boster.html2026-03-16T05:09:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00139-4-mmp9-primary-antibodies-ihc-testing-1.jpgAnti-MMP9 Rabbit Monoclonal AntibodyMouse lung tissue was stained with anti-MMP9 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00139-4-mmp9-primary-antibodies-ihc-testing-2.jpgAnti-MMP9 Rabbit Monoclonal AntibodyMouse spleen tissue was stained with anti-MMP9 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00139-4-mmp9-primary-antibodies-ihc-testing-3.jpgAnti-MMP9 Rabbit Monoclonal AntibodyRat spleen tissue was stained with anti-MMP9 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00139-4-mmp9-primary-antibodies-wb-testing-1.jpgAnti-MMP9 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MMP9 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse lung Lane 2: Rat lung Predicted band size: 78kDa Observed band size: 80-92kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rip-rabbit-monoclonal-antibody-m00141-3-boster.html2026-03-16T05:09:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00141-3-ripk1-primary-antibodies-ihc-testing-1.jpgAnti-RIP Rabbit Monoclonal AntibodyRat kidney was stained with Anti-RIP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00141-3-ripk1-primary-antibodies-ihc-testing-2.jpgAnti-RIP Rabbit Monoclonal AntibodyHuman cervical carcinoma was stained with anti-RIP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00141-3-ripk1-primary-antibodies-wb-testing-1.jpgAnti-RIP Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RIP antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HeLa Lane 3: Jurkat Lane 4: Raji Predicted band size: 76kDa Observed band size: 76kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd11b-rabbit-monoclonal-antibody-m00144-5-boster.html2026-03-16T05:09:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00144-5-itgam-primary-antibodies-ihc-testing-1.jpgAnti-CD11b Rabbit Monoclonal AntibodyMouse spleen was stained with anti-CD11b rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00144-5-itgam-primary-antibodies-ihc-testing-2.jpgAnti-CD11b Rabbit Monoclonal AntibodyRat spleen was stained with anti-CD11b rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00144-5-itgam-primary-antibodies-ihc-testing-3.jpgAnti-CD11b Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-CD11b rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00144-5-itgam-primary-antibodies-wb-testing-4.jpgAnti-CD11b Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD11b antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse spleen Predicted band size: 127kDa Observed band size: 170kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00144-5-itgam-primary-antibodies-wb-testing-5.jpgAnti-CD11b Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD11b antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse spleen Predicted band size: 127kDa Observed band size: 170kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p21waf1-cip1-rabbit-monoclonal-antibody-m00145-5-boster.html2026-03-16T05:09:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00145-5-cdkn1a-primary-antibodies-ihc-testing-1.jpgAnti-p21WAF1/Cip1 Rabbit Monoclonal AntibodyHuman tonsil tissue was stained with anti-p21WAF1/Cip1 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00145-5-cdkn1a-primary-antibodies-wb-testing-1.jpgAnti-p21WAF1/Cip1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-p21WAF1/Cip1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: MCF7 Predicted band size: 21kDa Observed band size: 21kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p21-rabbit-monoclonal-antibody-m00145-6-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00145-6-cdkn1a-primary-antibodies-ihc-testing-1.jpgAnti-p21 Rabbit Monoclonal AntibodyHuman cervical carcinoma was stained with anti-p21 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00145-6-cdkn1a-primary-antibodies-wb-testing-1.jpgAnti-p21 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p21 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HCT-116 Lane 2: MCF7 Lane 3: Rat spleen Predicted band size: 18kDa Observed band size: 18kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p21-rabbit-monoclonal-antibody-m00145-7-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00145-7-cdkn1a-primary-antibodies-ihc-testing-1.jpgAnti-p21 Rabbit Monoclonal AntibodyRat skin was stained with anti-p21 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00145-7-cdkn1a-primary-antibodies-wb-testing-1.jpgAnti-p21 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p21 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat womb Lane 2: RAW264.7 Predicted band size: 18kDa Observed band size: 18kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cyclin-d1-rabbit-monoclonal-antibody-m00149-5-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00149-5-ccnd1-primary-antibodies-ihc-testing-1.jpgAnti-Cyclin D1 Rabbit Monoclonal AntibodyHuman esophagus was stained with anti-Cyclin D1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00149-5-ccnd1-primary-antibodies-ihc-testing-2.jpgAnti-Cyclin D1 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Cyclin D1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00149-5-ccnd1-primary-antibodies-ihc-testing-3.jpgAnti-Cyclin D1 Rabbit Monoclonal AntibodyRat spleen was stained with anti-Cyclin D1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00149-5-ccnd1-primary-antibodies-ihc-testing-4.jpgAnti-Cyclin D1 Rabbit Monoclonal AntibodyMouse colon was stained with anti-Cyclin D1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00149-5-ccnd1-primary-antibodies-ihc-testing-5.jpgAnti-Cyclin D1 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-Cyclin D1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00149-5-ccnd1-primary-antibodies-wb-testing-1.jpgAnti-Cyclin D1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cyclin D1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mosue spleen Lane 2: Rat spleen Lane 3: LnCap Lane 4: MCF7 Predicted band size: 34kDa Observed band size: 36kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sh-ptp2-rabbit-monoclonal-antibody-m00150-5-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00150-5-ptpn11-primary-antibodies-wb-testing-1.jpgAnti-SH-PTP2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SH-PTP2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: 3T3-L1 Lane 4: A431 Predicted band size: 68kDa Observed band size: 68kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fak-rabbit-monoclonal-antibody-m00151-2-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00151-2-ptk2-primary-antibodies-ihc-testing-1.jpgAnti-FAK Rabbit Monoclonal AntibodyMouse spleen was stained with anti-FAK rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00151-2-ptk2-primary-antibodies-ihc-testing-2.jpgAnti-FAK Rabbit Monoclonal AntibodyRat spleen was stained with anti-FAK rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00151-2-ptk2-primary-antibodies-ihc-testing-3.jpgAnti-FAK Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-FAK rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00151-2-ptk2-primary-antibodies-wb-testing-1.jpgAnti-FAK Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FAK antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mosue brain Lane 2: Rat brain Lane 3: MCF7 Lane 4: HCT-116 Predicted band size: 119kDa Observed band size: 119kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nanog-rabbit-monoclonal-antibody-m00153-2-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00153-2-nanog-primary-antibodies-wb-testing-1.jpgAnti-Nanog Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Nanog antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NCCIT Predicted band size: 42kDa Observed band size: 42kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd19-rabbit-monoclonal-antibody-m00154-16-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00154-16-cd19-primary-antibodies-ihc-testing-1.jpgAnti-CD19 Rabbit Monoclonal AntibodyHuman tonsil tissue was stained with Anti-CD19 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00154-16-cd19-primary-antibodies-wb-testing-1.jpgAnti-CD19 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD19 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Raji Lane 2: Ramos Predicted band size: 110kDa Observed band size: 110kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cdk4-rabbit-monoclonal-antibody-m00159-3-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00159-3-cdk4-primary-antibodies-wb-testing-1.jpgAnti-CDK4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CDK4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: 3T3-L1 Predicted band size: 34kDa Observed band size: 34kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-irf3-rabbit-monoclonal-antibody-m00165-7-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00165-7-irf3-primary-antibodies-ihc-testing-1.jpgAnti-IRF3 Rabbit Monoclonal AntibodyRat stomach was stained with anti-IRF3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00165-7-irf3-primary-antibodies-ihc-testing-2.jpgAnti-IRF3 Rabbit Monoclonal AntibodyHuman stomach was stained with anti-IRF3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00165-7-irf3-primary-antibodies-wb-testing-1.jpgAnti-IRF3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IRF3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: THP-1 Lane 3: Mouse spleen Predicted band size: 47kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cdk2-rabbit-monoclonal-antibody-m00166-6-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-6-cdk2-primary-antibodies-ihc-testing-1.jpgAnti-CDK2 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-CDK2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00166-6-cdk2-primary-antibodies-wb-testing-1.jpgAnti-CDK2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CDK2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: Mouse spleen Lane 3: C6 Lane 4: HeLa Predicted band size: 34kDa Observed band size: 34kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p63-rabbit-monoclonal-antibody-m00167-4-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00167-4-tp63-primary-antibodies-wb-testing-1.jpgAnti-p63 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p63 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse skin Lane 2: PC-12 Predicted band size: 77kDa Observed band size: 77kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-icam1-rabbit-monoclonal-antibody-m00171-8-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00171-8-icam1-primary-antibodies-ihc-testing-1.jpgAnti-ICAM1 Rabbit Monoclonal AntibodyHuman kidney was stained with Anti-ICAM1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00171-8-icam1-primary-antibodies-wb-testing-1.jpgAnti-ICAM1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ICAM1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Raji Predicted band size: 58kDa Observed band size: 100kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dnmt1-rabbit-monoclonal-antibody-m00172-3-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00172-3-dnmt1-primary-antibodies-ihc-testing-1.jpgAnti-Dnmt1 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Dnmt1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00172-3-dnmt1-primary-antibodies-wb-testing-1.jpgAnti-Dnmt1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Dnmt1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Predicted band size: 183kDa Observed band size: 183kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p27-kip-1-rabbit-monoclonal-antibody-m00173-3-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00173-3-cdkn1b-primary-antibodies-ihc-testing-1.jpgAnti-p27 KIP 1 Rabbit Monoclonal AntibodyMouse brain was stained with anti-p27 KIP 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00173-3-cdkn1b-primary-antibodies-ihc-testing-2.jpgAnti-p27 KIP 1 Rabbit Monoclonal AntibodyRat brain was stained with anti-p27 KIP 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00173-3-cdkn1b-primary-antibodies-ihc-testing-3.jpgAnti-p27 KIP 1 Rabbit Monoclonal AntibodyHuman brain was stained with anti-p27 KIP 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00173-3-cdkn1b-primary-antibodies-wb-testing-1.jpgAnti-p27 KIP 1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p27 KIP 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Rat ovary Predicted band size: 27kDa Observed band size: 27kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-oct-4-rabbit-monoclonal-antibody-m00174-2-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00174-2-pou5f1-primary-antibodies-ihc-testing-1.jpgAnti-Oct-4 Rabbit Monoclonal AntibodyHuman seminoma was stained with anti-Oct-4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00174-2-pou5f1-primary-antibodies-wb-testing-1.jpgAnti-Oct-4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Oct-4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NCCIT Predicted band size: 39kDa Observed band size: 45kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p38-rabbit-monoclonal-antibody-m00176-4-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00176-4-mapk14-primary-antibodies-wb-testing-1.jpgAnti-p38 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p38 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: A549 Lane 3: Mouse lung Lane 4: Rat lung Predicted band size: 41kDa Observed band size: 41kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sox9-rabbit-monoclonal-antibody-m00177-2-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00177-2-sox9-primary-antibodies-ihc-testing-1.jpgAnti-SOX9 Rabbit Monoclonal AntibodyHuman colon was stained with anti-SOX9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00177-2-sox9-primary-antibodies-ihc-testing-2.jpgAnti-SOX9 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-SOX9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00177-2-sox9-primary-antibodies-ihc-testing-3.jpgAnti-SOX9 Rabbit Monoclonal AntibodyMouse colon was stained with anti-SOX9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00177-2-sox9-primary-antibodies-ihc-testing-4.jpgAnti-SOX9 Rabbit Monoclonal AntibodyRat colon was stained with anti-SOX9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00177-2-sox9-primary-antibodies-wb-testing-1.jpgAnti-SOX9 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SOX9 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: U-14 Lane 3: SW480 Predicted band size: 56kDa Observed band size: 70kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-caveolin-1-rabbit-monoclonal-antibody-m00179-4-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-4-cav1-primary-antibodies-ihc-testing-1.jpgAnti-Caveolin-1 Rabbit Monoclonal AntibodyHuman lung was stained with anti-Caveolin-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-4-cav1-primary-antibodies-ihc-testing-2.jpgAnti-Caveolin-1 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Caveolin-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-4-cav1-primary-antibodies-ihc-testing-3.jpgAnti-Caveolin-1 Rabbit Monoclonal AntibodyMouse lung was stained with anti-Caveolin-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00179-4-cav1-primary-antibodies-wb-testing-1.jpgAnti-Caveolin-1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Caveolin-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: A431 Lane 3: NIH-3T3 Lane 4: C6 Predicted band size: 20kDa Observed band size: 20kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bcl-xl-rabbit-monoclonal-antibody-m00181-4-boster.html2026-03-16T05:09:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00181-4-bcl2l1-primary-antibodies-ihc-testing-1.jpgAnti-Bcl-XL Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-Bcl-XL rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00181-4-bcl2l1-primary-antibodies-ihc-testing-2.jpgAnti-Bcl-XL Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-Bcl-XL rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00181-4-bcl2l1-primary-antibodies-wb-testing-1.jpgAnti-Bcl-XL Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Bcl-XL antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: C6 Lane 3: HeLa Lane 4: K562 Predicted band size: 26kDa Observed band size: 30kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bax-rabbit-monoclonal-antibody-m00183-6-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00183-6-bax-primary-antibodies-ihc-testing-1.jpgAnti-Bax Rabbit Monoclonal AntibodyMouse pancreas was stained with anti-Bax rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00183-6-bax-primary-antibodies-ihc-testing-2.jpgAnti-Bax Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Bax rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00183-6-bax-primary-antibodies-wb-testing-1.jpgAnti-Bax Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Bax antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: HeLa Lane 3: 3T3-L1 Lane 4: NIH-3T3 Predicted band size: 21kDa Observed band size: 21kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd38-rabbit-monoclonal-antibody-m00193-7-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00193-7-cd38-primary-antibodies-ihc-testing-1.jpgAnti-CD38 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-CD38 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00193-7-cd38-primary-antibodies-ihc-testing-2.jpgAnti-CD38 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-CD38 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00193-7-cd38-primary-antibodies-ihc-testing-3.jpgAnti-CD38 Rabbit Monoclonal AntibodyHuman appendix was stained with anti-CD38 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00193-7-cd38-primary-antibodies-ihc-testing-4.jpgAnti-CD38 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-CD38 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00193-7-cd38-primary-antibodies-wb-testing-1.jpgAnti-CD38 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD38 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: THP-1 Lane 2: A549 Predicted band size: 34kDa Observed band size: 45-65kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd38-rabbit-monoclonal-antibody-m00193-8-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00193-8-cd38-primary-antibodies-ihc-testing-1.jpgAnti-CD38 Rabbit Monoclonal AntibodyRat pancreas was stained with anti-CD38 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00193-8-cd38-primary-antibodies-ihc-testing-2.jpgAnti-CD38 Rabbit Monoclonal AntibodyMouse pancreas was stained with anti-CD38 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00193-8-cd38-primary-antibodies-wb-testing-1.jpgAnti-CD38 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD38 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse spleen Lane 2: Rat spleen Predicted band size: 34kDa Observed band size: 45kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wilms-tumor-protein-rabbit-monoclonal-antibody-m00199-2-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00199-2-wt1-primary-antibodies-ihc-testing-1.jpgAnti-Wilms Tumor Protein Rabbit Monoclonal AntibodyRat kidney was stained with anti-Wilms Tumor Protein rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00199-2-wt1-primary-antibodies-ihc-testing-2.jpgAnti-Wilms Tumor Protein Rabbit Monoclonal AntibodyMouse kidney was stained with anti-Wilms Tumor Protein rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00199-2-wt1-primary-antibodies-wb-testing-1.jpgAnti-Wilms Tumor Protein Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Wilms Tumor Protein antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse testis Lane 2: Rat testis Lane 3: THP-1 Predicted band size: 55kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rip3-rabbit-monoclonal-antibody-m00202-2-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00202-2-ripk3-primary-antibodies-wb-testing-1.jpgAnti-RIP3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RIP3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: J774A.1 Lane 2: L929 Predicted band size: 50kDa Observed band size: 50kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crebbp-rabbit-monoclonal-antibody-m00205-1-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00205-1-crebbp-primary-antibodies-ihc-testing-1.jpgAnti-CREBBP Rabbit Monoclonal AntibodyHuman pancreas was stained with anti-CREBBP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00205-1-crebbp-primary-antibodies-ihc-testing-2.jpgAnti-CREBBP Rabbit Monoclonal AntibodyMouse colon was stained with anti-CREBBP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00205-1-crebbp-primary-antibodies-wb-testing-1.jpgAnti-CREBBP Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-CREBBP antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Predicted band size: 300kDa Observed band size: 300kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rhoa-rabbit-monoclonal-antibody-m00207-4-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00207-4-rhoa-primary-antibodies-ihc-testing-1.jpgAnti-RhoA Rabbit Monoclonal AntibodyHuman kidney was stained with Anti-RhoA rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00207-4-rhoa-primary-antibodies-ihc-testing-2.jpgAnti-RhoA Rabbit Monoclonal AntibodyRat kidney was stained with Anti-RhoA rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00207-4-rhoa-primary-antibodies-wb-testing-1.jpgAnti-RhoA Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RhoA antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH3T3 Lane 2: HEK293 Predicted band size: 21kDa Observed band size: 21kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cdc2-rabbit-monoclonal-antibody-m00209-7-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00209-7-cdk1-primary-antibodies-ihc-testing-1.jpgAnti-Cdc2 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Cdc2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00209-7-cdk1-primary-antibodies-wb-testing-1.jpgAnti-Cdc2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cdc2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HeLa Predicted band size: 34kDa Observed band size: 34kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gfap-rabbit-monoclonal-antibody-m00213-10-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-10-gfap-primary-antibodies-ihc-testing-1.jpgAnti-GFAP Rabbit Monoclonal AntibodyRat brain was stained with anti-GFAP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-10-gfap-primary-antibodies-ihc-testing-2.jpgAnti-GFAP Rabbit Monoclonal AntibodyHuman brain was stained with anti-GFAP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-10-gfap-primary-antibodies-ihc-testing-3.jpgAnti-GFAP Rabbit Monoclonal AntibodyMouse brain was stained with anti-GFAP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00213-10-gfap-primary-antibodies-wb-testing-1.jpgAnti-GFAP Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GFAP antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse brain Lane 2: Rat brain Lane 3: U-251MG Predicted band size: 50kDa Observed band size: 50kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lkb1-rabbit-monoclonal-antibody-m00217-4-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00217-4-stk11-primary-antibodies-wb-testing-1.jpgAnti-LKB1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LKB1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: K562 Lane 3: L929 Lane 4: PC-12 Predicted band size: 49kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fto-rabbit-monoclonal-antibody-m00219-2-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-2-fto-primary-antibodies-ihc-testing-1.jpgAnti-FTO Rabbit Monoclonal AntibodyHuman kidney was stained with anti-FTO rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00219-2-fto-primary-antibodies-wb-testing-1.jpgAnti-FTO Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti- FTO antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: HeLa Lane 3: U-14 Lane 4: Rat ovary Predicted band size: 58kDa Observed band size: 58kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd86-rabbit-monoclonal-antibody-m00220-5-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00220-5-cd86-primary-antibodies-if-testing-1.jpgAnti-CD86 Rabbit Monoclonal AntibodyImmunofluorescence analysis of paraffin-embedded Mouse spleen. Primary Antibody was diluted at 1:200(4° overnight). an Multi colour-Fluorescence kit (RS0035, Immunoway). EDTA based antigen retrieval was used before Green tyramide signal amplification. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00220-5-cd86-primary-antibodies-ihc-testing-1.jpgAnti-CD86 Rabbit Monoclonal AntibodyMouse liver tissue was stained with Anti-CD86 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00220-5-cd86-primary-antibodies-ihc-testing-2.jpgAnti-CD86 Rabbit Monoclonal AntibodyMouse spleen tissue was stained with Anti-CD86 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00220-5-cd86-primary-antibodies-ihc-testing-3.jpgAnti-CD86 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-CD86 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00220-5-cd86-primary-antibodies-wb-testing-1.jpgAnti-CD86 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD86 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: J774A.1 Predicted band size: 35kDa Observed band size: 60-85kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-brg1-rabbit-monoclonal-antibody-m00223-3-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00223-3-smarca4-primary-antibodies-ihc-testing-1.jpgAnti-BRG1 Rabbit Monoclonal AntibodyRat stomach was stained with anti-BRG1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00223-3-smarca4-primary-antibodies-wb-testing-1.jpgAnti-BRG1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-BRG1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: HeLa Lane 3: HepG2 Predicted band size: 185kDa Observed band size: 220kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gapdh-rabbit-monoclonal-antibody-m00227-9-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00227-9-gapdh-primary-antibodies-wb-testing-1.jpgAnti-GAPDH Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GAPDH antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: GAPDH Lane 2: mouse testis Predicted band size: 37kDa Observed band size: 37kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gapdh-rabbit-monoclonal-antibody-m00227-10-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00227-10-gapdh-primary-antibodies-wb-testing-1.jpgAnti-GAPDH Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GAPDH antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: EL-4-B5 Lane 2: L6 Lane 3: JAR Lane 4: MDCK Lane 5: Cos-7 Predicted band size: 38kDa Observed band size: 38kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nucleolin-rabbit-monoclonal-antibody-m00228-4-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00228-4-ncl-primary-antibodies-ihc-testing-1.jpgAnti-Nucleolin Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Nucleolin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00228-4-ncl-primary-antibodies-wb-testing-1.jpgAnti-Nucleolin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Nucleolin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: HepG2 Predicted band size: 77kDa Observed band size: 110kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-xbp1-rabbit-monoclonal-antibody-m00234-3-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00234-3-xbp1-primary-antibodies-ihc-testing-1.jpgAnti-XBP1 Rabbit Monoclonal AntibodyRat spleen was stained with anti-XBP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00234-3-xbp1-primary-antibodies-ihc-testing-2.jpgAnti-XBP1 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-XBP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00234-3-xbp1-primary-antibodies-wb-testing-1.jpgAnti-XBP1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-XBP1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: MCF7 Lane 3: 4T1 Predicted band size: 29kDa Observed band size: 35, 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vimentin-rabbit-monoclonal-antibody-m00235-2-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00235-2-vim-primary-antibodies-ihc-testing-1.jpgAnti-Vimentin Rabbit Monoclonal AntibodyHuman colon was stained with anti-Vimentin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00235-2-vim-primary-antibodies-ihc-testing-2.jpgAnti-Vimentin Rabbit Monoclonal AntibodyMouse colon was stained with anti-Vimentin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00235-2-vim-primary-antibodies-ihc-testing-3.jpgAnti-Vimentin Rabbit Monoclonal AntibodyRat colon was stained with anti-Vimentin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00235-2-vim-primary-antibodies-wb-testing-1.jpgAnti-Vimentin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Vimentin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: U2OS Lane 3: C6 Lane 4: U-14 Predicted band size: 54kDa Observed band size: 54kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sod1-rabbit-monoclonal-antibody-m00238-3-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00238-3-sod1-primary-antibodies-ihc-testing-1.jpgAnti-SOD1 Rabbit Monoclonal AntibodyMouse brain was stained with anti-SOD1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00238-3-sod1-primary-antibodies-ihc-testing-2.jpgAnti-SOD1 Rabbit Monoclonal AntibodyHuman brain was stained with anti-SOD1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00238-3-sod1-primary-antibodies-ihc-testing-3.jpgAnti-SOD1 Rabbit Monoclonal AntibodyRat brain was stained with anti-SOD1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00238-3-sod1-primary-antibodies-wb-testing-1.jpgAnti-SOD1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SOD1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: HL-60 Lane 3: C6 Lane 4: Mouse liver Predicted band size: 23kDa Observed band size: 15kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atg5-rabbit-monoclonal-antibody-m00240-2-boster.html2026-03-16T05:09:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00240-2-atg5-primary-antibodies-ihc-testing-1.jpgAnti-ATG5 Rabbit Monoclonal AntibodyHuman colon was stained with anti-ATG5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00240-2-atg5-primary-antibodies-wb-testing-1.jpgAnti-ATG5 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ATG5 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-87 MG Lane 2: HeLa Lane 3: C6 Lane 4: 3T3-L1 Predicted band size: 33kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h2a-x-rabbit-monoclonal-antibody-m00241-3-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00241-3-h2afx-primary-antibodies-ihc-testing-1.jpgAnti-Histone H2A.X Rabbit Monoclonal AntibodyRat colon was stained with anti-Histone H2A.X (phospho Ser139) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00241-3-h2afx-primary-antibodies-ihc-testing-2.jpgAnti-Histone H2A.X Rabbit Monoclonal AntibodyMouse colon was stained with anti-Histone H2A.X (phospho Ser139) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00241-3-h2afx-primary-antibodies-ihc-testing-3.jpgAnti-Histone H2A.X Rabbit Monoclonal AntibodyMouse testis was stained with anti-Histone H2A.X (phospho Ser139) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00241-3-h2afx-primary-antibodies-wb-testing-1.jpgAnti-Histone H2A.X Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H2A.X (phospho Ser139) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 treated with UV for 30 minutes Lane 2: PC-12 Lane 3: NIH-3T3 Lane 4: HEK293 Predicted band size: 15kDa Observed band size: 15kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-btk-rabbit-monoclonal-antibody-m00245-4-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00245-4-btk-primary-antibodies-ihc-testing-1.jpgAnti-BTK Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-BTK rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00245-4-btk-primary-antibodies-wb-testing-1.jpgAnti-BTK Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-BTK antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: RAW264.7 Lane 2: K562 Lane 3: Ramos Lane 4: Rat thymus Predicted band size: 67kDa Observed band size: 67kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aurora-a-rabbit-monoclonal-antibody-m00246-7-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00246-7-aurka-primary-antibodies-wb-testing-1.jpgAnti-Aurora A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Aurora A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Mouse brain Lane 3: Rat brain Predicted band size: 45kDa Observed band size: 45kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-foxo3a-rabbit-monoclonal-antibody-m00252-2-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00252-2-foxo3-primary-antibodies-ihc-testing-1.jpgAnti-FOXO3A Rabbit Monoclonal AntibodyMouse pancreas was stained with anti-FOXO3A (phospho Ser253) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00252-2-foxo3-primary-antibodies-ihc-testing-2.jpgAnti-FOXO3A Rabbit Monoclonal AntibodyRat pancreas was stained with anti-FOXO3A (phospho Ser253) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00252-2-foxo3-primary-antibodies-ihc-testing-3.jpgAnti-FOXO3A Rabbit Monoclonal AntibodyHuman pancreas was stained with anti-FOXO3A (phospho Ser253) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00252-2-foxo3-primary-antibodies-wb-testing-1.jpgAnti-FOXO3A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FOXO3A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1:HEK293 Lane 2: HeLa Lane 3: Neuro-2a Lane 4: C6 Predicted band size: 71kDa Observed band size: 97kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ho-1-rabbit-monoclonal-antibody-m00253-3-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00253-3-hmox1-primary-antibodies-ihc-testing-1.jpgAnti-HO-1 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-HO-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00253-3-hmox1-primary-antibodies-ihc-testing-2.jpgAnti-HO-1 Rabbit Monoclonal AntibodyRat spleen was stained with anti-HO-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00253-3-hmox1-primary-antibodies-wb-testing-1.jpgAnti-HO-1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HO-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat spleen Lane 2: Mouse spleen Lane 3: A549 Predicted band size: 33kDa Observed band size: 33kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ki67-rabbit-monoclonal-antibody-m00254-10-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00254-10-mki67-primary-antibodies-ihc-testing-1.jpgAnti-Ki67 Rabbit Monoclonal AntibodyRat colon was stained with Anti-Ki67 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00254-10-mki67-primary-antibodies-ihc-testing-2.jpgAnti-Ki67 Rabbit Monoclonal AntibodyMouse colon was stained with Anti-Ki67 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00254-10-mki67-primary-antibodies-ihc-testing-3.jpgAnti-Ki67 Rabbit Monoclonal AntibodyHuman tonsil was stained with Anti-Ki67 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00254-10-mki67-primary-antibodies-ihc-testing-4.jpgAnti-Ki67 Rabbit Monoclonal AntibodyHuman appendix was stained with Anti-Ki67 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00254-10-mki67-primary-antibodies-ihc-testing-5.jpgAnti-Ki67 Rabbit Monoclonal AntibodyHuman tonsil tissue was stained with Anti-Ki67 Antibody (green)https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00254-10-mki67-primary-antibodies-wb-testing-1.jpgAnti-Ki67 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Ki67 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Predicted band size: 359kDa Observed band size: 359kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ucp1-rabbit-monoclonal-antibody-m00255-1-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00255-1-ucp1-primary-antibodies-wb-testing-1.jpgAnti-UCP1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-UCP1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse white adipose Lane 2: Rat white adipose Predicted band size: 33kDa Observed band size: 33kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hdac1-rabbit-monoclonal-antibody-m00256-2-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00256-2-hdac1-primary-antibodies-ihc-testing-1.jpgAnti-HDAC1 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-HDAC1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00256-2-hdac1-primary-antibodies-ihc-testing-2.jpgAnti-HDAC1 Rabbit Monoclonal AntibodyMouse liver was stained with anti-HDAC1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00256-2-hdac1-primary-antibodies-ihc-testing-3.jpgAnti-HDAC1 Rabbit Monoclonal AntibodyRat liver was stained with anti-HDAC1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00256-2-hdac1-primary-antibodies-wb-testing-1.jpgAnti-HDAC1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HDAC1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HeLa Lane 3: NIH-3T3 Lane 4: C6 Predicted band size: 55kDa Observed band size: 62kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-e2f-1-rabbit-monoclonal-antibody-m00257-1-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00257-1-e2f1-primary-antibodies-ihc-testing-1.jpgAnti-E2F-1 Rabbit Monoclonal AntibodyMouse pancreas was stained with anti-E2F-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00257-1-e2f1-primary-antibodies-ihc-testing-2.jpgAnti-E2F-1 Rabbit Monoclonal AntibodyRat pancreas was stained with anti-E2F-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00257-1-e2f1-primary-antibodies-wb-testing-1.jpgAnti-E2F-1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-E2F-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-3 Lane 2: Rat heart Lane 3: NIH-3T3 Lane 4: HT-1080 Predicted band size: 47kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tbk1-rabbit-monoclonal-antibody-m00261-1-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00261-1-tbk1-primary-antibodies-ihc-testing-1.jpgAnti-TBK1 Rabbit Monoclonal AntibodyHuman testis was stained with anti-TBK1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00261-1-tbk1-primary-antibodies-ihc-testing-2.jpgAnti-TBK1 Rabbit Monoclonal AntibodyRat liver was stained with anti-TBK1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00261-1-tbk1-primary-antibodies-ihc-testing-3.jpgAnti-TBK1 Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-TBK1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00261-1-tbk1-primary-antibodies-wb-testing-1.jpgAnti-TBK1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TBK1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Jurkat Lane 3: C6 Predicted band size: 84kDa Observed band size: 84kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hnf-3alpha-rabbit-monoclonal-antibody-m00266-4-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00266-4-foxa1-primary-antibodies-ihc-testing-1.jpgAnti-HNF-3α Rabbit Monoclonal AntibodyMouse colon was stained with anti-HNF-3α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00266-4-foxa1-primary-antibodies-ihc-testing-2.jpgAnti-HNF-3α Rabbit Monoclonal AntibodyRat colon was stained with anti-HNF-3α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00266-4-foxa1-primary-antibodies-ihc-testing-3.jpgAnti-HNF-3α Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-HNF-3α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00266-4-foxa1-primary-antibodies-ihc-testing-4.jpgAnti-HNF-3α Rabbit Monoclonal AntibodyHuman colon was stained with anti-HNF-3α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00266-4-foxa1-primary-antibodies-wb-testing-1.jpgAnti-HNF-3α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HNF-3α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MCF7 Lane 2: HepG2 Lane 3: 4T1 Predicted band size: 49kDa Observed band size: 49kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mitf-rabbit-monoclonal-antibody-m00269-4-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00269-4-mitf-primary-antibodies-ihc-testing-1.jpgAnti-MiTF Rabbit Monoclonal AntibodyMouse placenta was stained with Anti-MiTF rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00269-4-mitf-primary-antibodies-ihc-testing-2.jpgAnti-MiTF Rabbit Monoclonal AntibodyHuman melanoma was stained with Anti-MiTF rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00269-4-mitf-primary-antibodies-wb-testing-1.jpgAnti-MiTF Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MiTF antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A375 Lane 2: U-14 Lane 3: Rat womb Predicted band size: 58kDa Observed band size: 58kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vwf-rabbit-monoclonal-antibody-m00270-6-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00270-6-vwf-primary-antibodies-ihc-testing-1.jpgAnti-VWF Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-VWF rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00270-6-vwf-primary-antibodies-ihc-testing-2.jpgAnti-VWF Rabbit Monoclonal AntibodyRat spleen was stained with anti-VWF rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00270-6-vwf-primary-antibodies-wb-testing-3.jpgAnti-VWF Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti- VWF antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Predicted band size: 309kDa Observed band size: 280kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00270-6-vwf-primary-antibodies-wb-testing-4.jpgAnti-VWF Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-VWF antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat liver Predicted band size: 309kDa Observed band size: 280kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pax6-rabbit-monoclonal-antibody-m00273-5-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00273-5-pax6-primary-antibodies-ihc-testing-1.jpgAnti-PAX6 Rabbit Monoclonal AntibodyMouse brain was stained with anti-PAX6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00273-5-pax6-primary-antibodies-wb-testing-1.jpgAnti-PAX6 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PAX6 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HeLa Lane 3: U-14 Predicted band size: 47kDa Observed band size: 47kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-epcam-rabbit-monoclonal-antibody-m00276-7-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00276-7-epcam-primary-antibodies-ihc-testing-1.jpgAnti-EpCAM Rabbit Monoclonal AntibodyRat colon was stained with anti-EpCAM rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00276-7-epcam-primary-antibodies-ihc-testing-2.jpgAnti-EpCAM Rabbit Monoclonal AntibodyHuman liver was stained with anti-EpCAM rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00276-7-epcam-primary-antibodies-ihc-testing-3.jpgAnti-EpCAM Rabbit Monoclonal AntibodyMouse colon was stained with anti-EpCAM rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00276-7-epcam-primary-antibodies-wb-testing-1.jpgAnti-EpCAM Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-EpCAM antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HCT-116 Lane 2: MCF7 Predicted band size: 35kDa Observed band size: 40kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chk2-rabbit-monoclonal-antibody-m00277-3-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00277-3-chek2-primary-antibodies-ihc-testing-1.jpgAnti-Chk2 Rabbit Monoclonal AntibodyHuman colon was stained with anti-Chk2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00277-3-chek2-primary-antibodies-wb-testing-1.jpgAnti-Chk2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Chk2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HeLa Predicted band size: 61kDa Observed band size: 61kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chk2-rabbit-monoclonal-antibody-m00277-4-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00277-4-chek2-primary-antibodies-ihc-testing-1.jpgAnti-Chk2 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-Chk2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00277-4-chek2-primary-antibodies-wb-testing-1.jpgAnti-Chk2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Chk2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MDA-MB-231 Lane 2: U-14 Lane 3: HeLa Predicted band size: 61kDa Observed band size: 61kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nf-kb1-p105-p50-rabbit-monoclonal-antibody-m00283-3-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00283-3-nfkb1-primary-antibodies-ihc-testing-1.jpgAnti-NF-κB1 p105/p50 Rabbit Monoclonal AntibodyHuman prostate carcinoma was stained with anti-NF-κB1 p105/p50 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00283-3-nfkb1-primary-antibodies-wb-testing-1.jpgAnti-NF-κB1 p105/p50 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NF-κB1 p105/p50 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A20 Lane 2: THP-1 Lane 3: Rat spleen Lane 4: Mouse spleen Predicted band size: 50,105kDa Observed band size: 50,120kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nf-kb-p65-rabbit-monoclonal-antibody-m00284-4-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00284-4-rela-primary-antibodies-ihc-testing-1.jpgAnti-NF-κB p65 Rabbit Monoclonal AntibodyMouse colon was stained with anti-NF-κB p65 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00284-4-rela-primary-antibodies-ihc-testing-2.jpgAnti-NF-κB p65 Rabbit Monoclonal AntibodyHuman colon was stained with anti-NF-κB p65 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00284-4-rela-primary-antibodies-ihc-testing-3.jpgAnti-NF-κB p65 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-NF-κB p65 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00284-4-rela-primary-antibodies-wb-testing-1.jpgAnti-NF-κB p65 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NF-κB p65 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-12 Lane 2: HeLa Lane 3: K562 Lane 4: 3T3-L1 Predicted band size: 65kDa Observed band size: 70kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mmp2-rabbit-monoclonal-antibody-m00286-2-boster.html2026-03-16T05:09:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00286-2-mmp2-primary-antibodies-wb-testing-1.jpgAnti-MMP2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MMP2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: L6 Lane 2: U-87 MG Lane 3: HT-1080 Predicted band size: 74kDa Observed band size: 64kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mek1-rabbit-monoclonal-antibody-m00292-5-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00292-5-map2k1-primary-antibodies-ihc-testing-1.jpgAnti-MEK1 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-MEK1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00292-5-map2k1-primary-antibodies-ihc-testing-2.jpgAnti-MEK1 Rabbit Monoclonal AntibodyHuman lung was stained with anti-MEK1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00292-5-map2k1-primary-antibodies-wb-testing-1.jpgAnti-MEK1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MEK1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: Jurkat Lane 3: Mouse skin Lane 4: C6 Predicted band size: 43kDa Observed band size: 43kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mek1-2-rabbit-monoclonal-antibody-m00292-6-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00292-6-map2k1-primary-antibodies-ihc-testing-1.jpgAnti-MEK1/2 Rabbit Monoclonal AntibodyHuman lung carcinoma was stained with anti-MEK1/2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00292-6-map2k1-primary-antibodies-ihc-testing-2.jpgAnti-MEK1/2 Rabbit Monoclonal AntibodyMouse colon was stained with anti-MEK1/2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00292-6-map2k1-primary-antibodies-wb-testing-1.jpgAnti-MEK1/2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti- MEK1/2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: 3T3-L1 Lane 4: K562 Predicted band size: 44kDa Observed band size: 44kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c-fos-rabbit-monoclonal-antibody-m00297-7-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00297-7-fos-primary-antibodies-wb-testing-1.jpgAnti-c-Fos Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-c-Fos antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa treated by Phorbol 12-myristate 13-acetate(TPA) with 24 hours Lane 2: MCF7 Lane 3: U-14 Predicted band size: 41kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sqstm1-p62-rabbit-monoclonal-antibody-m00300-5-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-5-sqstm1-primary-antibodies-ihc-testing-1.jpgAnti-SQSTM1/p62 Rabbit Monoclonal AntibodyMouse spleen tissue was stained with Anti-SQSTM1/p62 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-5-sqstm1-primary-antibodies-ihc-testing-2.jpgAnti-SQSTM1/p62 Rabbit Monoclonal AntibodyRat spleen tissue was stained with Anti-SQSTM1/p62 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-5-sqstm1-primary-antibodies-ihc-testing-3.jpgAnti-SQSTM1/p62 Rabbit Monoclonal AntibodyHuman colon carcinoma tissue was stained with Anti-SQSTM1/p62 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00300-5-sqstm1-primary-antibodies-wb-testing-1.jpgAnti-SQSTM1/p62 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SQATM1/P62 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: PANC-1 Lane 2: RT-4 Lane 3: PC12 Predicted band size: 48kDa Observed band size: 62kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alk-rabbit-monoclonal-antibody-m00301-5-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00301-5-alk-primary-antibodies-ihc-testing-1.jpgAnti-ALK Rabbit Monoclonal AntibodyHuman non-hodgkin lymphoma was stained with anti-ALK rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00301-5-alk-primary-antibodies-ihc-testing-2.jpgAnti-ALK Rabbit Monoclonal AntibodyHuman neuroblastoma was stained with anti-ALK rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00301-5-alk-primary-antibodies-wb-testing-1.jpgAnti-ALK Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-ALK antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: Karpas-299 Predicted band size: 177kDa Observed band size: 220kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tata-box-binding-protein-rabbit-monoclonal-antibody-m00302-2-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00302-2-tbp-primary-antibodies-ihc-testing-1.jpgAnti-TATA Box Binding Protein Rabbit Monoclonal AntibodyMouse colon was stained with Anti-TATA Box Binding Protein rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00302-2-tbp-primary-antibodies-ihc-testing-2.jpgAnti-TATA Box Binding Protein Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with Anti-TATA Box Binding Protein rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00302-2-tbp-primary-antibodies-ihc-testing-3.jpgAnti-TATA Box Binding Protein Rabbit Monoclonal AntibodyHuman colon was stained with Anti-TATA Box Binding Protein rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00302-2-tbp-primary-antibodies-ihc-testing-4.jpgAnti-TATA Box Binding Protein Rabbit Monoclonal AntibodyRat colon was stained with Anti-TATA Box Binding Protein rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00302-2-tbp-primary-antibodies-wb-testing-1.jpgAnti-TATA Box Binding Protein Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TATA Box Binding Protein antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HeLa Lane 3: U-14 Lane 4: Rat womb Predicted band size: 38kDa Observed band size: 38kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vasp-rabbit-monoclonal-antibody-m00303-3-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00303-3-vasp-primary-antibodies-ihc-testing-1.jpgAnti-VASP Rabbit Monoclonal AntibodyHuman kidney was stained with anti-VASP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00303-3-vasp-primary-antibodies-ihc-testing-2.jpgAnti-VASP Rabbit Monoclonal AntibodyHuman placenta was stained with anti-VASP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00303-3-vasp-primary-antibodies-ihc-testing-3.jpgAnti-VASP Rabbit Monoclonal AntibodyMouse kidney was stained with anti-VASP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00303-3-vasp-primary-antibodies-ihc-testing-4.jpgAnti-VASP Rabbit Monoclonal AntibodyRat kidney was stained with anti-VASP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00303-3-vasp-primary-antibodies-wb-testing-1.jpgAnti-VASP Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-VASP antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Jurkat Predicted band size: 40kDa Observed band size: 50kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chop-rabbit-monoclonal-antibody-m00311-2-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00311-2-ddit3-primary-antibodies-ihc-testing-1.jpgAnti-CHOP Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-CHOP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00311-2-ddit3-primary-antibodies-wb-testing-1.jpgAnti-CHOP Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CHOP antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: HepG2 Lane 3: MCF7 Predicted band size: 19kDa Observed band size: 30kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stromal-interaction-molecule-1-rabbit-monoclonal-antibody-m00312-2-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00312-2-stim1-primary-antibodies-ihc-testing-1.jpgAnti-Stromal Interaction Molecule 1 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-Stromal Interaction Molecule 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00312-2-stim1-primary-antibodies-ihc-testing-2.jpgAnti-Stromal Interaction Molecule 1 Rabbit Monoclonal AntibodyHuman breast was stained with anti-Stromal Interaction Molecule 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00312-2-stim1-primary-antibodies-ihc-testing-3.jpgAnti-Stromal Interaction Molecule 1 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-Stromal Interaction Molecule 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00312-2-stim1-primary-antibodies-wb-testing-1.jpgAnti-Stromal Interaction Molecule 1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Stromal Interaction Molecule 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: HeLa Lane 3: U-14 Lane 4: A20 Predicted band size: 77kDa Observed band size: 77kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pi3-kinase-p85-alpha-rabbit-monoclonal-antibody-m00318-3-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00318-3-pik3r1-primary-antibodies-ihc-testing-1.jpgAnti-PI3-Kinase p85 α Rabbit Monoclonal AntibodyMouse testis was stained with Anti-PI3-Kinase p85 α rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00318-3-pik3r1-primary-antibodies-ihc-testing-2.jpgAnti-PI3-Kinase p85 α Rabbit Monoclonal AntibodyHuman placenta was stained with Anti-PI3-Kinase p85 α rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00318-3-pik3r1-primary-antibodies-wb-testing-1.jpgAnti-PI3-Kinase p85 α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PI3-Kinase p85 α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HepG2 Lane 3: Mouse brain Lane 4: Rat brain Predicted band size: 84kDa Observed band size: 84kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hdac2-rabbit-monoclonal-antibody-m00325-3-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00325-3-hdac2-primary-antibodies-ihc-testing-1.jpgAnti-HDAC2 Rabbit Monoclonal AntibodyMouse colon was stained with Anti-HDAC2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00325-3-hdac2-primary-antibodies-ihc-testing-2.jpgAnti-HDAC2 Rabbit Monoclonal AntibodyRat spleen was stained with Anti-HDAC2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00325-3-hdac2-primary-antibodies-ihc-testing-3.jpgAnti-HDAC2 Rabbit Monoclonal AntibodyHuman tonsil was stained with Anti-HDAC2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00325-3-hdac2-primary-antibodies-wb-testing-1.jpgAnti-HDAC2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HDAC2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: HeLa Lane 3: NIH-3T3 Lane 4: K562 Lane 5: A431 Lane 6: HepG2 Predicted band size: 55kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-beclin-1-rabbit-monoclonal-antibody-m00327-4-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-4-becn1-primary-antibodies-ihc-testing-1.jpgAnti-Beclin 1 Rabbit Monoclonal AntibodyRat kidney was stained with anti-Beclin 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-4-becn1-primary-antibodies-ihc-testing-2.jpgAnti-Beclin 1 Rabbit Monoclonal AntibodyMouse kidney was stained with anti-Beclin 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00327-4-becn1-primary-antibodies-wb-testing-1.jpgAnti-Beclin 1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Beclin 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: K562 Lane 3: C6 Lane 4: 3T3-L1 Predicted band size: 52kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nox2-rabbit-monoclonal-antibody-m00328-1-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00328-1-cybb-primary-antibodies-wb-testing-1.jpgAnti-NOX2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NOX2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: Jurkat Lane 3: Mouse brain Predicted band size: 65kDa Observed band size: 65kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-caspase-3-rabbit-monoclonal-antibody-m00334-10-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-10-casp3-primary-antibodies-ihc-testing-1.jpgAnti-Caspase-3 Rabbit Monoclonal AntibodyHuman tonsil was stained with Anti-Caspase-3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-10-casp3-primary-antibodies-ihc-testing-2.jpgAnti-Caspase-3 Rabbit Monoclonal AntibodyRat spleen was stained with Anti-Caspase-3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-10-casp3-primary-antibodies-ihc-testing-3.jpgAnti-Caspase-3 Rabbit Monoclonal AntibodyMouse spleen was stained with Anti-Caspase-3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-10-casp3-primary-antibodies-wb-testing-1.jpgAnti-Caspase-3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Caspase-3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Mouse spleen Predicted band size: 35kDa Observed band size: 35kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cleaved-caspase-3-rabbit-monoclonal-antibody-m00334-11-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00334-11-casp3-primary-antibodies-wb-testing-1.jpgAnti-Cleaved Caspase-3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cleaved Caspase-3 (Asp175) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: NIH-3T3 tearted with Apopida Predicted band size: 17,19kDa Observed band size: 17,19kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bmp2-rabbit-monoclonal-antibody-m00338-1-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00338-1-bmp2-primary-antibodies-wb-testing-1.jpgAnti-BMP2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-BMP2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: MCF7 Lane 3: RAW264.7 Lane 4: Rat spleen Predicted band size: 45,13kDa Observed band size: 30,13kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd4-rabbit-monoclonal-antibody-m00344-9-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00344-9-cd4-primary-antibodies-ihc-testing-1.jpgAnti-CD4 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-CD4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00344-9-cd4-primary-antibodies-ihc-testing-2.jpgAnti-CD4 Rabbit Monoclonal AntibodyHuman colon was stained with anti-CD4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00344-9-cd4-primary-antibodies-wb-testing-1.jpgAnti-CD4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: THP-1 Lane 2: Jurkat Lane 2: Mouse spleen Predicted band size: 51kDa Observed band size: 51kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd4-rabbit-monoclonal-antibody-m00344-10-boster.html2026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00344-10-cd4-primary-antibodies-ihc-testing-1.jpgAnti-CD4 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-CD4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00344-10-cd4-primary-antibodies-wb-testing-1.jpgAnti-CD4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse thymus Predicted band size: 51kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atg7-rabbit-monoclonal-antibody-m00346-3-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00346-3-atg7-primary-antibodies-ihc-testing-1.jpgAnti-ATG7 Rabbit Monoclonal AntibodyHuman cervical carcinoma was stained with anti-ATG7 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00346-3-atg7-primary-antibodies-wb-testing-1.jpgAnti-ATG7 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti- ATG7 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Jurkat Lane 3: 3T3-L1 Lane 4: C6 Predicted band size: 78kDa Observed band size: 78kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sod2-rabbit-monoclonal-antibody-m00349-5-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-5-sod2-primary-antibodies-ihc-testing-1.jpgAnti-SOD2 Rabbit Monoclonal AntibodyHuman colon was stained with anti-SOD2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-5-sod2-primary-antibodies-ihc-testing-2.jpgAnti-SOD2 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-SOD2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-5-sod2-primary-antibodies-ihc-testing-3.jpgAnti-SOD2 Rabbit Monoclonal AntibodyMouse kidney was stained with anti-SOD2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-5-sod2-primary-antibodies-ihc-testing-4.jpgAnti-SOD2 Rabbit Monoclonal AntibodyRat kidney was stained with anti-SOD2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00349-5-sod2-primary-antibodies-wb-testing-1.jpgAnti-SOD2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SOD2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Mouse brain Lane 3: Rat brain Predicted band size: 25kDa Observed band size: 22kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-granzyme-b-rabbit-monoclonal-antibody-m00353-3-boster.html2026-03-16T05:09:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00353-3-gzmb-primary-antibodies-ihc-testing-1.jpgAnti-Granzyme B Rabbit Monoclonal AntibodyHuman spleen was stained with anti-Granzyme B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00353-3-gzmb-primary-antibodies-wb-testing-1.jpgAnti-Granzyme B Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Granzyme B antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Karpas-299 Lane 2: NK92 Predicted band size: 28kDa Observed band size: 37kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cdk6-rabbit-monoclonal-antibody-m00358-2-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00358-2-cdk6-primary-antibodies-ihc-testing-1.jpgAnti-CDK6 Rabbit Monoclonal AntibodyRat colon tonsil was stained with Anti-CDK6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00358-2-cdk6-primary-antibodies-ihc-testing-2.jpgAnti-CDK6 Rabbit Monoclonal AntibodyMouse colon was stained with Anti-CDK6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00358-2-cdk6-primary-antibodies-ihc-testing-3.jpgAnti-CDK6 Rabbit Monoclonal AntibodyHuman tonsil was stained with Anti-CDK6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00358-2-cdk6-primary-antibodies-wb-testing-1.jpgAnti-CDK6 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CDK6 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Jurkat Lane 3: K562 Predicted band size: 37kDa Observed band size: 37kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-asc-rabbit-monoclonal-antibody-m00362-3-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00362-3-pycard-primary-antibodies-wb-testing-1.jpgAnti-ASC Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ASC antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse spleen Predicted band size: 22kDa Observed band size: 22kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pdgfr-alpha-rabbit-monoclonal-antibody-m00366-6-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00366-6-pdgfra-primary-antibodies-wb-testing-1.jpgAnti-PDGFR-α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PDGFR-α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MG-63 Lane 2: Rat heart Predicted band size: 123kDa Observed band size: 190kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atf-4-rabbit-monoclonal-antibody-m00371-2-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00371-2-atf4-primary-antibodies-ihc-testing-1.jpgAnti-ATF-4 Rabbit Monoclonal AntibodyMouse lung was stained with anti-ATF-4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00371-2-atf4-primary-antibodies-ihc-testing-2.jpgAnti-ATF-4 Rabbit Monoclonal AntibodyRat lung was stained with anti-ATF-4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00371-2-atf4-primary-antibodies-wb-testing-1.jpgAnti-ATF-4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ATF-4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1:Hela treated with serum for 30min Predicted band size: 38kDa Observed band size: 49kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-survivin-rabbit-monoclonal-antibody-m00379-3-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00379-3-birc5-primary-antibodies-wb-testing-1.jpgAnti-Survivin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Survivin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: Neuro-2a Predicted band size: 16kDa Observed band size: 16kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-53bp1-rabbit-monoclonal-antibody-m00397-3-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00397-3-tp53bp1-primary-antibodies-wb-testing-1.jpgAnti-53BP1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-53BP1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Predicted band size: 214kDa Observed band size: 450kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytokeratin-5-rabbit-monoclonal-antibody-m00398-9-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00398-9-krt5-primary-antibodies-ihc-testing-1.jpgAnti-Cytokeratin 5 Rabbit Monoclonal AntibodyHuman lung was stained with anti-Cytokeratin 5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00398-9-krt5-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 5 Rabbit Monoclonal AntibodyMouse lung was stained with anti-Cytokeratin 5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00398-9-krt5-primary-antibodies-ihc-testing-3.jpgAnti-Cytokeratin 5 Rabbit Monoclonal AntibodyRat lung was stained with anti-Cytokeratin 5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00398-9-krt5-primary-antibodies-ihc-testing-4.jpgAnti-Cytokeratin 5 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Cytokeratin 5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00398-9-krt5-primary-antibodies-wb-testing-5.jpgAnti-Cytokeratin 5 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 5 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: HaCat Lane 3: Mouse skin Predicted band size: 62kDa Observed band size: 62kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00398-9-krt5-primary-antibodies-wb-testing-6.jpgAnti-Cytokeratin 5 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 5 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HaCat Lane 2: Mouse skin Lane 3: Rat skin Predicted band size: 62kDa Observed band size: 62kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nadph-oxidase-4-rabbit-monoclonal-antibody-m00403-1-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00403-1-nox4-primary-antibodies-wb-testing-1.jpgAnti-NADPH oxidase 4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NADPH antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: U-87MG Predicted band size: 67kDa Observed band size: 67kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kap1-rabbit-monoclonal-antibody-m00409-4-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-4-trim28-primary-antibodies-ihc-testing-1.jpgAnti-KAP1 Rabbit Monoclonal AntibodyMouse colon was stained with anti-KAP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-4-trim28-primary-antibodies-ihc-testing-2.jpgAnti-KAP1 Rabbit Monoclonal AntibodyRat colon was stained with anti-KAP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-4-trim28-primary-antibodies-ihc-testing-3.jpgAnti-KAP1 Rabbit Monoclonal AntibodyHuman colon was stained with anti-KAP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-4-trim28-primary-antibodies-ihc-testing-4.jpgAnti-KAP1 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-KAP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00409-4-trim28-primary-antibodies-wb-testing-1.jpgAnti-KAP1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-KAP1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HeLa Lane 3: Rat womb Lane 4: U-14 Predicted band size: 89kDa Observed band size: 110kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mif-rabbit-monoclonal-antibody-m00411-2-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00411-2-mif-primary-antibodies-ihc-testing-1.jpgAnti-MIF Rabbit Monoclonal AntibodyHuman thymus was stained with anti-MIF rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00411-2-mif-primary-antibodies-wb-testing-1.jpgAnti-MIF Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MIF antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: 3T3-L1 Lane 3: Jurkat Lane 4: Y79 Predicted band size: 13kDa Observed band size: 13kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mmp13-rabbit-monoclonal-antibody-m00420-2-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00420-2-mmp13-primary-antibodies-wb-testing-1.jpgAnti-MMP13 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MMP13 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Predicted band size: 60kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fap-rabbit-monoclonal-antibody-m00422-2-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00422-2-fap-primary-antibodies-ihc-testing-1.jpgAnti-FAP Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-FAP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00422-2-fap-primary-antibodies-ihc-testing-2.jpgAnti-FAP Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-FAP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00422-2-fap-primary-antibodies-wb-testing-1.jpgAnti-FAP Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-FAP antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MRC-5 Predicted band size: 87kDa Observed band size: 87kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lck-rabbit-monoclonal-antibody-m00425-1-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00425-1-lck-primary-antibodies-ihc-testing-1.jpgAnti-Lck Rabbit Monoclonal AntibodyRat colon was stained with anti-Lck rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00425-1-lck-primary-antibodies-ihc-testing-2.jpgAnti-Lck Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Lck rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00425-1-lck-primary-antibodies-wb-testing-1.jpgAnti-Lck Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Lck antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: Ramos Lane 3: Mouse spleen Predicted band size: 58kDa Observed band size: 58kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lamin-a-c-rabbit-monoclonal-antibody-m00438-8-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00438-8-lmna-primary-antibodies-ihc-testing-1.jpgAnti-Lamin A/C Rabbit Monoclonal AntibodyHuman liver was stained with anti-Lamin A/C rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00438-8-lmna-primary-antibodies-ihc-testing-2.jpgAnti-Lamin A/C Rabbit Monoclonal AntibodyMouse liver was stained with anti-Lamin A/C rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00438-8-lmna-primary-antibodies-ihc-testing-3.jpgAnti-Lamin A/C Rabbit Monoclonal AntibodyRat liver was stained with anti-Lamin A/C rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00438-8-lmna-primary-antibodies-wb-testing-1.jpgAnti-Lamin A/C Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Lamin A /C antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HaCat Lane 2: Rat womb Lane 3: 3T3-L1 Predicted band size: 74,63kDa Observed band size: 74,63kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-runx2-rabbit-monoclonal-antibody-m00442-2-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00442-2-runx2-primary-antibodies-ihc-testing-1.jpgAnti-RUNX2 Rabbit Monoclonal AntibodyHuman osteosarcoma was stained with anti-RUNX2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00442-2-runx2-primary-antibodies-ihc-testing-2.jpgAnti-RUNX2 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-RUNX2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00442-2-runx2-primary-antibodies-wb-testing-1.jpgAnti-RUNX2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RUNX2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MDA-MB-231 Lane 2: Saos-2 Lane 3: NIH-3T3 Lane 4: Rat womb Predicted band size: 57kDa Observed band size: 57kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pak1-rabbit-monoclonal-antibody-m00454-1-boster.html2026-03-16T05:09:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00454-1-pak1-primary-antibodies-ihc-testing-1.jpgAnti-PAK1 Rabbit Monoclonal AntibodyHuman brain was stained with anti-PAK1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00454-1-pak1-primary-antibodies-ihc-testing-2.jpgAnti-PAK1 Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-PAK1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00454-1-pak1-primary-antibodies-ihc-testing-3.jpgAnti-PAK1 Rabbit Monoclonal AntibodyRat brain was stained with anti-PAK1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00454-1-pak1-primary-antibodies-wb-testing-1.jpgAnti-PAK1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PAK1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: Jurkat Lane 4: U-14 Predicted band size: 61kDa Observed band size: 61kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mitofusin-2-rabbit-monoclonal-antibody-m00461-1-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00461-1-mfn2-primary-antibodies-wb-testing-1.jpgAnti-Mitofusin-2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Mitofusin-2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: SK-MEL-28 Lane 3: Mouse kidney Predicted band size: 86kDa Observed band size: 86kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-xiap-rabbit-monoclonal-antibody-m00482-2-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00482-2-xiap-primary-antibodies-wb-testing-1.jpgAnti-XIAP Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-XIAP antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: C6 Lane 3: HT-29 Lane 4: K562 Predicted band size: 57kDa Observed band size: 57kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-syk-rabbit-monoclonal-antibody-m00490-1-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00490-1-syk-primary-antibodies-ihc-testing-1.jpgAnti-Syk Rabbit Monoclonal AntibodyHuman spleen was stained with anti-Syk rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00490-1-syk-primary-antibodies-ihc-testing-2.jpgAnti-Syk Rabbit Monoclonal AntibodyMouse spleen was stained with anti-Syk rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00490-1-syk-primary-antibodies-ihc-testing-3.jpgAnti-Syk Rabbit Monoclonal AntibodyRat spleen was stained with anti-Syk rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00490-1-syk-primary-antibodies-wb-testing-1.jpgAnti-Syk Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Syk antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SW620 Lane 2: A20 Lane 3: THP-1 Predicted band size: 72kDa Observed band size: 65kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nqo1-rabbit-monoclonal-antibody-m00494-3-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00494-3-nqo1-primary-antibodies-ihc-testing-1.jpgAnti-NQO1 Rabbit Monoclonal AntibodyRat kidney was stained with anti-NQO1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00494-3-nqo1-primary-antibodies-ihc-testing-2.jpgAnti-NQO1 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-NQO1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00494-3-nqo1-primary-antibodies-ihc-testing-3.jpgAnti-NQO1 Rabbit Monoclonal AntibodyMouse kidney was stained with anti-NQO1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00494-3-nqo1-primary-antibodies-wb-testing-1.jpgAnti-NQO1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NQO1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: NIH-3T3 Lane 3: Rat kidney Lane 4: HepG2 Predicted band size: 31kDa Observed band size: 31kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-filamin-a-rabbit-monoclonal-antibody-m00502-3-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00502-3-flna-primary-antibodies-ihc-testing-1.jpgAnti-Filamin A Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-Filamin A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00502-3-flna-primary-antibodies-wb-testing-1.jpgAnti-Filamin A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Filamin A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: Mouse liver Predicted band size: 281kDa Observed band size: 281kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glucocorticoid-receptor-rabbit-monoclonal-antibody-m00503-4-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00503-4-nr3c1-primary-antibodies-ihc-testing-1.jpgAnti-Glucocorticoid Receptor Rabbit Monoclonal AntibodyRat liver was stained with anti-Glucocorticoid Receptor rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00503-4-nr3c1-primary-antibodies-ihc-testing-2.jpgAnti-Glucocorticoid Receptor Rabbit Monoclonal AntibodyHuman liver was stained with anti-Glucocorticoid Receptor rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00503-4-nr3c1-primary-antibodies-ihc-testing-3.jpgAnti-Glucocorticoid Receptor Rabbit Monoclonal AntibodyMouse liver was stained with anti-Glucocorticoid Receptor rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00503-4-nr3c1-primary-antibodies-wb-testing-1.jpgAnti-Glucocorticoid Receptor Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Glucocorticoid Receptor antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-251 MG Lane 2: A549 Lane 3: RAW264.7 Predicted band size: 85kDa Observed band size: 94kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adiponectin-rabbit-monoclonal-antibody-m00509-5-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00509-5-adipoq-primary-antibodies-ihc-testing-1.jpgAnti-Adiponectin Rabbit Monoclonal AntibodyRat adipose was stained with anti-Adiponectin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00509-5-adipoq-primary-antibodies-ihc-testing-2.jpgAnti-Adiponectin Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Adiponectin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00509-5-adipoq-primary-antibodies-ihc-testing-3.jpgAnti-Adiponectin Rabbit Monoclonal AntibodyHuman placenta was stained with anti-Adiponectin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00509-5-adipoq-primary-antibodies-ihc-testing-4.jpgAnti-Adiponectin Rabbit Monoclonal AntibodyMouse adipose was stained with anti-Adiponectin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00509-5-adipoq-primary-antibodies-wb-testing-1.jpgAnti-Adiponectin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Adiponectin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat fat Lane 2: Mouse brown adipose Predicted band size: 26kDa Observed band size: 30kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-keap1-rabbit-monoclonal-antibody-m00514-1-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00514-1-keap1-primary-antibodies-ihc-testing-1.jpgAnti-Keap1 Rabbit Monoclonal AntibodyMouse brain was stained with anti-Keap1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00514-1-keap1-primary-antibodies-ihc-testing-2.jpgAnti-Keap1 Rabbit Monoclonal AntibodyRat brain was stained with anti-Keap1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00514-1-keap1-primary-antibodies-ihc-testing-3.jpgAnti-Keap1 Rabbit Monoclonal AntibodyHuman brain was stained with anti-Keap1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00514-1-keap1-primary-antibodies-ihc-testing-4.jpgAnti-Keap1 Rabbit Monoclonal AntibodyHuman prostate was stained with anti-Keap1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00514-1-keap1-primary-antibodies-wb-testing-1.jpgAnti-Keap1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Keap1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: RAW264.7 Lane 3: Rat spleen Predicted band size: 70kDa Observed band size: 60-70kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-notch2-rabbit-monoclonal-antibody-m00518-1-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00518-1-notch2-primary-antibodies-ihc-testing-1.jpgAnti-Notch2 Rabbit Monoclonal AntibodyHuman liver was stained with anti-Notch2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00518-1-notch2-primary-antibodies-ihc-testing-2.jpgAnti-Notch2 Rabbit Monoclonal AntibodyHuman testis was stained with anti-Notch2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00518-1-notch2-primary-antibodies-ihc-testing-3.jpgAnti-Notch2 Rabbit Monoclonal AntibodyMouse liver was stained with anti-Notch2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00518-1-notch2-primary-antibodies-ihc-testing-4.jpgAnti-Notch2 Rabbit Monoclonal AntibodyRat liver was stained with anti-Notch2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00518-1-notch2-primary-antibodies-wb-testing-5.jpgAnti-Notch2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Notch2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: 4T1 Predicted band size: 265kDa Observed band size: 120kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00518-1-notch2-primary-antibodies-wb-testing-6.jpgAnti-Notch2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Notch2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat womb Predicted band size: 265kDa Observed band size: 120kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stat6-rabbit-monoclonal-antibody-m00523-3-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00523-3-stat6-primary-antibodies-ihc-testing-1.jpgAnti-STAT6 Rabbit Monoclonal AntibodyMouse kidney was stained with anti-STAT6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00523-3-stat6-primary-antibodies-ihc-testing-2.jpgAnti-STAT6 Rabbit Monoclonal AntibodyRat kidney was stained with anti-STAT6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00523-3-stat6-primary-antibodies-ihc-testing-3.jpgAnti-STAT6 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-STAT6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00523-3-stat6-primary-antibodies-wb-testing-1.jpgAnti-STAT6 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-STAT6 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Jurkat Lane 3: Mouse thymus Predicted band size: 94kDa Observed band size: 110kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kdm1a-rabbit-monoclonal-antibody-m00532-3-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00532-3-kdm1a-primary-antibodies-ihc-testing-1.jpgAnti-KDM1A Rabbit Monoclonal AntibodyMouse colon was stained with anti-KDM1A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00532-3-kdm1a-primary-antibodies-ihc-testing-2.jpgAnti-KDM1A Rabbit Monoclonal AntibodyRat colon was stained with anti-KDM1A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00532-3-kdm1a-primary-antibodies-ihc-testing-3.jpgAnti-KDM1A Rabbit Monoclonal AntibodyHuman prostate was stained with anti-KDM1A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00532-3-kdm1a-primary-antibodies-ihc-testing-4.jpgAnti-KDM1A Rabbit Monoclonal AntibodyHuman prostate carcinoma was stained with anti-KDM1A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00532-3-kdm1a-primary-antibodies-wb-testing-1.jpgAnti-KDM1A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-KDM1A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: HeLa Lane 3: Jurkat Lane 4: U-14 Predicted band size: 93kDa Observed band size: 110kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vegf-receptor-1-rabbit-monoclonal-antibody-m00534-5-boster.html2026-03-27T05:07:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00534-5-flt1-primary-antibodies-ihc-testing-1.jpgAnti-VEGF Receptor 1 Rabbit Monoclonal AntibodyHuman gastric carcinoma was stained with anti-VEGF Receptor 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00534-5-flt1-primary-antibodies-wb-testing-1.jpgAnti-VEGF Receptor 1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-VEGF Receptor 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Predicted band size: 151kDa Observed band size: 180kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mlkl-rabbit-monoclonal-antibody-m00535-2-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00535-2-mlkl-primary-antibodies-ihc-testing-1.jpgAnti-MLKL Rabbit Monoclonal AntibodyHumantonsil was stained with anti-MLKL rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00535-2-mlkl-primary-antibodies-wb-testing-1.jpgAnti-MLKL Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MLKL antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: U-251 MG Lane 3: U-14 Lane 4: Rat womb Predicted band size: 55kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-erbb-3-rabbit-monoclonal-antibody-m00539-10-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00539-10-erbb3-primary-antibodies-wb-testing-1.jpgAnti-ErbB-3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-ErbB-3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SK-BR-3 Predicted band size: 148kDa Observed band size: 185kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00539-10-erbb3-primary-antibodies-wb-testing-2.jpgAnti-ErbB-3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ErbB-3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 4T1 Lane 2: T47D Lane 3: Rat skin Predicted band size: 148kDa Observed band size: 185kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cyclin-e1-rabbit-monoclonal-antibody-m00543-4-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00543-4-ccne1-primary-antibodies-ihc-testing-1.jpgAnti-Cyclin E1 Rabbit Monoclonal AntibodyRat colon was stained with Anti-Cyclin E1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00543-4-ccne1-primary-antibodies-ihc-testing-2.jpgAnti-Cyclin E1 Rabbit Monoclonal AntibodyHuman colon was stained with Anti-Cyclin E1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00543-4-ccne1-primary-antibodies-wb-testing-1.jpgAnti-Cyclin E1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cyclin E1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Predicted band size: 47kDa Observed band size: 47kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-zeb1-rabbit-monoclonal-antibody-m00548-3-boster.html2026-03-16T05:09:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-3-zeb1-primary-antibodies-ihc-testing-1.jpgAnti-ZEB1 Rabbit Monoclonal AntibodyRat colon was stained with anti-ZEB1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-3-zeb1-primary-antibodies-ihc-testing-2.jpgAnti-ZEB1 Rabbit Monoclonal AntibodyHuman colon was stained with anti-ZEB1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00548-3-zeb1-primary-antibodies-wb-testing-1.jpgAnti-ZEB1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-ZEB1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A459 Lane 2: HeLa Predicted band size: 124kDa Observed band size: 200kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-isg15-rabbit-monoclonal-antibody-m00554-3-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00554-3-isg15-primary-antibodies-wb-testing-1.jpgAnti-ISG15 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ISG15 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: PC-3 Predicted band size: 18kDa Observed band size: 18kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd45-rabbit-monoclonal-antibody-m00555-10-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00555-10-ptprc-primary-antibodies-ihc-testing-1.jpgAnti-CD45 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-CD45 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00555-10-ptprc-primary-antibodies-wb-testing-1.jpgAnti-CD45 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-CD45 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Predicted band size: 147kDa Observed band size: 240-200kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-drp1-rabbit-monoclonal-antibody-m00556-3-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00556-3-dnm1l-primary-antibodies-ihc-testing-1.jpgAnti-DRP1 Rabbit Monoclonal AntibodyHuman brain was stained with Anti-DRP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00556-3-dnm1l-primary-antibodies-ihc-testing-2.jpgAnti-DRP1 Rabbit Monoclonal AntibodyMouse brain was stained with Anti-DRP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00556-3-dnm1l-primary-antibodies-ihc-testing-3.jpgAnti-DRP1 Rabbit Monoclonal AntibodyRat brain was stained with Anti-DRP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00556-3-dnm1l-primary-antibodies-ihc-testing-4.jpgAnti-DRP1 Rabbit Monoclonal AntibodyMouse pancreas was stained with anti-DRP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00556-3-dnm1l-primary-antibodies-ihc-testing-5.jpgAnti-DRP1 Rabbit Monoclonal AntibodyRat pancreas was stained with anti-DRP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00556-3-dnm1l-primary-antibodies-wb-testing-1.jpgAnti-DRP1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DRP1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat brain Lane 2: Mouse brain Predicted band size: 83kDa Observed band size: 83kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fibronectin-rabbit-monoclonal-antibody-m00564-7-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00564-7-fn1-primary-antibodies-ihc-testing-1.jpgAnti-Fibronectin Rabbit Monoclonal AntibodyHuman breast was stained with anti-Fibronectin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00564-7-fn1-primary-antibodies-ihc-testing-2.jpgAnti-Fibronectin Rabbit Monoclonal AntibodyHuman liver was stained with anti-Fibronectin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00564-7-fn1-primary-antibodies-ihc-testing-3.jpgAnti-Fibronectin Rabbit Monoclonal AntibodyMouse liver was stained with anti-Fibronectin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00564-7-fn1-primary-antibodies-wb-testing-1.jpgAnti-Fibronectin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Fibronectin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Predicted band size: 263kDa Observed band size: 285kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-creb-1-rabbit-monoclonal-antibody-m00577-1-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00577-1-creb1-primary-antibodies-ihc-testing-1.jpgAnti-CREB-1 Rabbit Monoclonal AntibodyMouse brain was stained with anti-CREB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00577-1-creb1-primary-antibodies-ihc-testing-2.jpgAnti-CREB-1 Rabbit Monoclonal AntibodyRat brain was stained with anti-CREB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00577-1-creb1-primary-antibodies-ihc-testing-3.jpgAnti-CREB-1 Rabbit Monoclonal AntibodyHuman brain was stained with anti-CREB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00577-1-creb1-primary-antibodies-ihc-testing-4.jpgAnti-CREB-1 Rabbit Monoclonal AntibodyHuman thyroid carcinoma was stained with anti-CREB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00577-1-creb1-primary-antibodies-wb-testing-1.jpgAnti-CREB-1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CREB-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: HEK293 Lane 3: C6 Lane 4: NIH-3T3 Predicted band size: 37kDa Observed band size: 43kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-polycomb-protein-suz12-rabbit-monoclonal-antibody-m00583-3-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00583-3-suz12-primary-antibodies-ihc-testing-1.jpgAnti-Polycomb Protein SUZ12 Rabbit Monoclonal AntibodyHuman kidney was stained with Anti-Polycomb Protein SUZ12 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00583-3-suz12-primary-antibodies-ihc-testing-2.jpgAnti-Polycomb Protein SUZ12 Rabbit Monoclonal AntibodyMouse kidney was stained with Anti-Polycomb Protein SUZ12 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00583-3-suz12-primary-antibodies-ihc-testing-3.jpgAnti-Polycomb Protein SUZ12 Rabbit Monoclonal AntibodyRat kidney was stained with Anti-Polycomb Protein SUZ12 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00583-3-suz12-primary-antibodies-wb-testing-1.jpgAnti-Polycomb Protein SUZ12 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Polycomb Protein SUZ12 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1:A549 Lane 2: C2C12 Lane 3: MCF7 Lane 4: L6 Predicted band size: 83kDa Observed band size: 83kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-integrin-beta3-rabbit-monoclonal-antibody-m00587-3-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00587-3-itgb3-primary-antibodies-ihc-testing-1.jpgAnti-Integrin β3 Rabbit Monoclonal AntibodyHuman osteosarcomas was stained with anti-Integrin β3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00587-3-itgb3-primary-antibodies-ihc-testing-2.jpgAnti-Integrin β3 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-Integrin β3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00587-3-itgb3-primary-antibodies-ihc-testing-3.jpgAnti-Integrin β3 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-Integrin β3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00587-3-itgb3-primary-antibodies-ihc-testing-4.jpgAnti-Integrin β3 Rabbit Monoclonal AntibodyRat spleen was stained with anti-Integrin β3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00587-3-itgb3-primary-antibodies-wb-testing-1.jpgAnti-Integrin β3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Integrin β3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse spleen Lane 2: Rat spleen Predicted band size: 87kDa Observed band size: 100kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-transferrin-receptor-rabbit-monoclonal-antibody-m00591-4-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00591-4-tfrc-primary-antibodies-ihc-testing-1.jpgAnti-Transferrin Receptor Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Transferrin Receptor rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00591-4-tfrc-primary-antibodies-ihc-testing-2.jpgAnti-Transferrin Receptor Rabbit Monoclonal AntibodyHuman placenta was stained with anti-Transferrin Receptor rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00591-4-tfrc-primary-antibodies-ihc-testing-3.jpgAnti-Transferrin Receptor Rabbit Monoclonal AntibodyMouse kidney was stained with anti-Transferrin Receptor rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00591-4-tfrc-primary-antibodies-ihc-testing-4.jpgAnti-Transferrin Receptor Rabbit Monoclonal AntibodyRat kidney was stained with anti-Transferrin Receptor rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00591-4-tfrc-primary-antibodies-wb-testing-1.jpgAnti-Transferrin Receptor Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Transferrin Receptor antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HeLa Predicted band size: 84kDa Observed band size: 84kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-connexin-43-rabbit-monoclonal-antibody-m00599-1-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00599-1-gja1-primary-antibodies-ihc-testing-1.jpgAnti-Connexin 43 Rabbit Monoclonal AntibodyHuman testis was stained with anti-Connexin 43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00599-1-gja1-primary-antibodies-ihc-testing-2.jpgAnti-Connexin 43 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-Connexin 43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00599-1-gja1-primary-antibodies-ihc-testing-3.jpgAnti-Connexin 43 Rabbit Monoclonal AntibodyRat spleen was stained with anti-Connexin 43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00599-1-gja1-primary-antibodies-wb-testing-1.jpgAnti-Connexin 43 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Connexin 43 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat womb Lane 2: HEK293 Lane 3: HeLa Lane 4: C2C12 Predicted band size: 43kDa Observed band size: 43kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd68-rabbit-monoclonal-antibody-m00602-4-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00602-4-cd68-primary-antibodies-if-testing-1.jpgAnti-CD68 Rabbit Monoclonal AntibodyFluorescence multiplex immunohistochemical analysis of human tonsil tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Sextuple-Fluorescence kit (RS0039, Immunoway).CD68 rabbit mAb(RED) and CD163 mouse mAb(GREEN) was tested with different TSA Fluorescence regent. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00602-4-cd68-primary-antibodies-ihc-testing-1.jpgAnti-CD68 Rabbit Monoclonal AntibodyHuman liver was stained with Anti-CD68 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00602-4-cd68-primary-antibodies-ihc-testing-2.jpgAnti-CD68 Rabbit Monoclonal AntibodyHuman appendix was stained with Anti-CD68 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00602-4-cd68-primary-antibodies-wb-testing-1.jpgAnti-CD68 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD68 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-87MG Predicted band size: 35kDa Observed band size: 120kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd68-rabbit-monoclonal-antibody-m00602-5-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00602-5-cd68-primary-antibodies-ihc-testing-1.jpgAnti-CD68 Rabbit Monoclonal AntibodyMouse liver was stained with anti-CD68 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00602-5-cd68-primary-antibodies-ihc-testing-2.jpgAnti-CD68 Rabbit Monoclonal AntibodyRat liver was stained with anti-CD68 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00602-5-cd68-primary-antibodies-wb-testing-1.jpgAnti-CD68 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD68 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse brain Lane 2: Rat brain Predicted band size: 37kDa Observed band size: 100kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lef1-rabbit-monoclonal-antibody-m00605-3-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00605-3-lef1-primary-antibodies-ihc-testing-1.jpgAnti-LEF1 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-LEF1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00605-3-lef1-primary-antibodies-ihc-testing-2.jpgAnti-LEF1 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-LEF1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00605-3-lef1-primary-antibodies-ihc-testing-3.jpgAnti-LEF1 Rabbit Monoclonal AntibodyRat spleen was stained with anti-LEF1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00605-3-lef1-primary-antibodies-ihc-testing-4.jpgAnti-LEF1 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-LEF1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00605-3-lef1-primary-antibodies-wb-testing-1.jpgAnti-LEF1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LEF1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: Mouse thymus Predicted band size: 44kDa Observed band size: 45-60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sumo-1-rabbit-monoclonal-antibody-m00631-2-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00631-2-sumo1-primary-antibodies-ihc-testing-1.jpgAnti-Sumo 1 Rabbit Monoclonal AntibodyHuman lung carcinoma was stained with anti-Sumo 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00631-2-sumo1-primary-antibodies-wb-testing-1.jpgAnti-Sumo 1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Sumo 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: HeLa Lane 3: PC-12 Lane 4: NIH-3T3 Predicted band size: 12kDa Observed band size: 80kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-osteopontin-rabbit-monoclonal-antibody-m00634-3-boster.html2026-03-16T05:09:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00634-3-spp1-primary-antibodies-ihc-testing-1.jpgAnti-Osteopontin Rabbit Monoclonal AntibodyHuman liver was stained with anti-Osteopontin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00634-3-spp1-primary-antibodies-wb-testing-1.jpgAnti-Osteopontin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Osteopontin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: HepG2 Predicted band size: 35kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dna-pkcs-rabbit-monoclonal-antibody-m00645-3-boster.html2026-03-16T05:09:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00645-3-prkdc-primary-antibodies-wb-testing-1.jpgAnti-DNA PKcs Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DNA PKcs antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HT-29 Lane 2: A549 Predicted band size: 469kDa Observed band size: 469kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prkaca-rabbit-monoclonal-antibody-m00653-1-boster.html2026-03-16T05:09:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00653-1-prkaca-primary-antibodies-ihc-testing-1.jpgAnti-PRKACA Rabbit Monoclonal AntibodyHuman thyroid carcinoma was stained with anti-PRKACA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00653-1-prkaca-primary-antibodies-ihc-testing-2.jpgAnti-PRKACA Rabbit Monoclonal AntibodyMouse stomach was stained with anti-PRKACA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00653-1-prkaca-primary-antibodies-ihc-testing-3.jpgAnti-PRKACA Rabbit Monoclonal AntibodyRat stomach was stained with anti-PRKACA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00653-1-prkaca-primary-antibodies-wb-testing-1.jpgAnti-PRKACA Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PRKACA antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: 3T3-L1 Predicted band size: 40kDa Observed band size: 40kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atf6a-rabbit-monoclonal-antibody-m00655-1-boster.html2026-03-16T05:09:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00655-1-atf6-primary-antibodies-ihc-testing-1.jpgAnti-ATF6A Rabbit Monoclonal AntibodyRat kidney was stained with anti-ATF6A PT® Rabbit mAbhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00655-1-atf6-primary-antibodies-ihc-testing-2.jpgAnti-ATF6A Rabbit Monoclonal AntibodyHuman kidney was stained with anti-ATF6A PT® Rabbit mAbhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00655-1-atf6-primary-antibodies-ihc-testing-3.jpgAnti-ATF6A Rabbit Monoclonal AntibodyMouse kidney was stained with anti-ATF6A PT® Rabbit mAbhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00655-1-atf6-primary-antibodies-wb-testing-1.jpgAnti-ATF6A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ATF6A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: U2OS Predicted band size: 75Da Observed band size: 100kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pax5-rabbit-monoclonal-antibody-m00669-4-boster.html2026-03-16T05:09:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00669-4-pax5-primary-antibodies-ihc-testing-1.jpgAnti-PAX5 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-PAX5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00669-4-pax5-primary-antibodies-ihc-testing-2.jpgAnti-PAX5 Rabbit Monoclonal AntibodyRat spleen was stained with anti-PAX5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00669-4-pax5-primary-antibodies-wb-testing-1.jpgAnti-PAX5 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PAX5 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Ramos Lane 2: Daudi Lane 3: Mouse spleen Lane 4: Rat spleen Predicted band size: 42kDa Observed band size: 42kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ddx5-rabbit-monoclonal-antibody-m00670-3-boster.html2026-03-16T05:09:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-3-ddx5-primary-antibodies-ihc-testing-1.jpgAnti-DDX5 Rabbit Monoclonal AntibodyHuman bladder carcinoma was stained with anti-DDX5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-3-ddx5-primary-antibodies-ihc-testing-2.jpgAnti-DDX5 Rabbit Monoclonal AntibodyHuman colon was stained with anti-DDX5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00670-3-ddx5-primary-antibodies-wb-testing-1.jpgAnti-DDX5 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DDX5 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: 3T3-L1 Lane 4: U-87 MG Predicted band size: 69kDa Observed band size: 69kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ire1alpha-rabbit-monoclonal-antibody-m00683-1-boster.html2026-03-16T05:09:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00683-1-ern1-primary-antibodies-ihc-testing-1.jpgAnti-IRE1α Rabbit Monoclonal AntibodyHuman brain was stained with anti-IRE1α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00683-1-ern1-primary-antibodies-wb-testing-1.jpgAnti-IRE1α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IRE1α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: LnCap Lane 2: Rat Prostate Predicted band size: 110kDa Observed band size: 110kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cyclin-a2-rabbit-monoclonal-antibody-m00700-3-boster.html2026-03-16T05:09:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00700-3-ccna2-primary-antibodies-ihc-testing-1.jpgAnti-Cyclin A2 Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-Cyclin A2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00700-3-ccna2-primary-antibodies-ihc-testing-2.jpgAnti-Cyclin A2 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Cyclin A2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00700-3-ccna2-primary-antibodies-wb-testing-1.jpgAnti-Cyclin A2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cyclin A2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: C6 Lane 3: Mouse spleen Lane 4: HeLa Predicted band size: 49kDa Observed band size: 49kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trk-pan-rabbit-monoclonal-antibody-m00706-4-boster.html2026-03-16T05:09:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00706-4-ntrk1-primary-antibodies-wb-testing-1.jpgAnti-Trk pan Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Trk pan antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: IMR-32 Lane 2: Neuro-2a Lane 3: Mouse brain Lane 4: Rat brain Predicted band size: 87kDa Observed band size: 120-140kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cgas-rabbit-monoclonal-antibody-m00709-1-boster.html2026-03-16T05:09:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00709-1-mb21d1-primary-antibodies-wb-testing-1.jpgAnti-cGAS Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-cGAS antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HT-29 Lane 2: HeLa Predicted band size: 62kDa Observed band size: 62kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mucin-5ac-rabbit-monoclonal-antibody-m00719-1-boster.html2026-03-16T05:09:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00719-1-muc5b-primary-antibodies-ihc-testing-1.jpgAnti-Mucin 5AC Rabbit Monoclonal AntibodyMouse stomach was stained with anti-Mucin 5AC rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00719-1-muc5b-primary-antibodies-ihc-testing-2.jpgAnti-Mucin 5AC Rabbit Monoclonal AntibodyRat stomach was stained with anti-Mucin 5AC rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00719-1-muc5b-primary-antibodies-ihc-testing-3.jpgAnti-Mucin 5AC Rabbit Monoclonal AntibodyHuman stomach was stained with anti-Mucin 5AC rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00719-1-muc5b-primary-antibodies-wb-testing-1.jpgAnti-Mucin 5AC Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Mucin 5AC antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HT-29 Predicted band size: 586kDa Observed band size: 586kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rock1-rabbit-monoclonal-antibody-m00722-2-boster.html2026-03-16T05:09:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00722-2-rock1-primary-antibodies-ihc-testing-1.jpgAnti-ROCK1 Rabbit Monoclonal AntibodyHuman testis was stained with anti-ROCK1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00722-2-rock1-primary-antibodies-wb-testing-1.jpgAnti-ROCK1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-ROCK1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Predicted band size: 158kDa Observed band size: 158kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pkc-alpha-rabbit-monoclonal-antibody-m00743-6-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00743-6-prkca-primary-antibodies-ihc-testing-1.jpgAnti-PKC α Rabbit Monoclonal AntibodyMouse liver was stained with anti-PKC α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00743-6-prkca-primary-antibodies-ihc-testing-2.jpgAnti-PKC α Rabbit Monoclonal AntibodyRat liver was stained with anti-PKC α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00743-6-prkca-primary-antibodies-ihc-testing-3.jpgAnti-PKC α Rabbit Monoclonal AntibodyHuman liver was stained with anti-PKC α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00743-6-prkca-primary-antibodies-ihc-testing-4.jpgAnti-PKC α Rabbit Monoclonal AntibodyHuman small intestine was stained with anti-PKC α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00743-6-prkca-primary-antibodies-wb-testing-1.jpgAnti-PKC α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PKC α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: 3T3-L1 Lane 3: C6 Predicted band size: 77kDa Observed band size: 77kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ace2-rabbit-monoclonal-antibody-m00756-2-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00756-2-ace2-primary-antibodies-ihc-testing-1.jpgAnti-ACE2 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-ACE2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00756-2-ace2-primary-antibodies-ihc-testing-2.jpgAnti-ACE2 Rabbit Monoclonal AntibodyHuman testis was stained with anti-ACE2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00756-2-ace2-primary-antibodies-ihc-testing-3.jpgAnti-ACE2 Rabbit Monoclonal AntibodyRat kidney was stained with anti-ACE2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00756-2-ace2-primary-antibodies-wb-testing-1.jpgAnti-ACE2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ACE2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Caco-2 Predicted band size: 130kDa Observed band size: 130kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sox10-rabbit-monoclonal-antibody-m00758-3-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00758-3-sox10-primary-antibodies-ihc-testing-1.jpgAnti-SOX10 Rabbit Monoclonal AntibodyHumanmelanoma was stained with anti-SOX10 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00758-3-sox10-primary-antibodies-ihc-testing-2.jpgAnti-SOX10 Rabbit Monoclonal AntibodyHumanbreastcarcinoma was stained with anti-SOX10 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00758-3-sox10-primary-antibodies-wb-testing-1.jpgAnti-SOX10 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SOX10 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: U-14 Lane 3: A375 Predicted band size: 50kDa Observed band size: 62kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tgf-beta-receptor-ii-rabbit-monoclonal-antibody-m00759-5-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00759-5-tgfbr2-primary-antibodies-ihc-testing-1.jpgAnti-TGF β Receptor II Rabbit Monoclonal AntibodyHuman placenta was stained with anti-TGF β Receptor II rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00759-5-tgfbr2-primary-antibodies-wb-testing-1.jpgAnti-TGF β Receptor II Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TGF β Receptor II antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Mouse lung Lane 2: Rat lung Predicted band size: 65kDa Observed band size: 65kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aurora-b-rabbit-monoclonal-antibody-m00762-5-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00762-5-aurkb-primary-antibodies-ihc-testing-1.jpgAnti-Aurora B Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Aurora B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00762-5-aurkb-primary-antibodies-wb-testing-1.jpgAnti-Aurora B Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Aurora B antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: MG-63 Lane 3: L929 Predicted band size: 39kDa Observed band size: 39kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tls-fus-rabbit-monoclonal-antibody-m00771-2-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00771-2-fus-primary-antibodies-ihc-testing-1.jpgAnti-TLS/FUS Rabbit Monoclonal AntibodyMouse brain was stained with anti-TLS/FUS rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00771-2-fus-primary-antibodies-ihc-testing-2.jpgAnti-TLS/FUS Rabbit Monoclonal AntibodyRat brain was stained with anti-TLS/FUS rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00771-2-fus-primary-antibodies-wb-testing-1.jpgAnti-TLS/FUS Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TLS/FUS antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: SH-SY5Y Predicted band size: 53kDa Observed band size: 70kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-integrin-beta1-rabbit-monoclonal-antibody-m00772-6-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00772-6-itgb1-primary-antibodies-ihc-testing-1.jpgAnti-Integrin β1 Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-Integrin β1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00772-6-itgb1-primary-antibodies-ihc-testing-2.jpgAnti-Integrin β1 Rabbit Monoclonal AntibodyRat kidney was stained with anti-Integrin β1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00772-6-itgb1-primary-antibodies-wb-testing-3.jpgAnti-Integrin β1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Integrin β1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: C6 Predicted band size: 88kDa Observed band size: 115-125kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00772-6-itgb1-primary-antibodies-wb-testing-4.jpgAnti-Integrin β1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Integrin β1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Predicted band size: 88kDa Observed band size: 115-125kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mmp3-rabbit-monoclonal-antibody-m00775-1-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00775-1-mmp3-primary-antibodies-ihc-testing-1.jpgAnti-MMP3 Rabbit Monoclonal AntibodyMouse liver was stained with anti-MMP3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00775-1-mmp3-primary-antibodies-ihc-testing-2.jpgAnti-MMP3 Rabbit Monoclonal AntibodyRat liver was stained with anti-MMP3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00775-1-mmp3-primary-antibodies-ihc-testing-3.jpgAnti-MMP3 Rabbit Monoclonal AntibodyHuman liver was stained with anti-MMP3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00775-1-mmp3-primary-antibodies-wb-testing-1.jpgAnti-MMP3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MMP3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse brain Lane 2: Rat brain Predicted band size: 54kDa Observed band size: 54kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lamp1-rabbit-monoclonal-antibody-m00780-6-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00780-6-lamp1-primary-antibodies-ihc-testing-1.jpgAnti-LAMP1 Rabbit Monoclonal AntibodyMouse liver was stained with anti-LAMP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00780-6-lamp1-primary-antibodies-ihc-testing-2.jpgAnti-LAMP1 Rabbit Monoclonal AntibodyRat liver was stained with anti-LAMP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00780-6-lamp1-primary-antibodies-ihc-testing-3.jpgAnti-LAMP1 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-LAMP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00780-6-lamp1-primary-antibodies-ihc-testing-4.jpgAnti-LAMP1 Rabbit Monoclonal AntibodyHuman liver was stained with anti-LAMP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00780-6-lamp1-primary-antibodies-wb-testing-1.jpgAnti-LAMP1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LAMP1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Jurkat Predicted band size: 45kDa Observed band size: 100kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-collagen-iii-rabbit-monoclonal-antibody-m00788-2-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-2-col3a1-primary-antibodies-ihc-testing-1.jpgAnti-Collagen III Rabbit Monoclonal AntibodyRat skin was stained with anti-Collagen III rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-2-col3a1-primary-antibodies-ihc-testing-2.jpgAnti-Collagen III Rabbit Monoclonal AntibodyHuman skin was stained with anti-Collagen III rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-2-col3a1-primary-antibodies-wb-testing-3.jpgAnti-Collagen III Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Collagen III antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-14 Predicted band size: 138kDa Observed band size: 150kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00788-2-col3a1-primary-antibodies-wb-testing-4.jpgAnti-Collagen III Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Collagen III antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Rat womb Lane 3: A431 Predicted band size: 138kDa Observed band size: 150kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gsk3-beta-rabbit-monoclonal-antibody-m00791-1-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00791-1-gsk3b-primary-antibodies-ihc-testing-1.jpgAnti-GSK3 β Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with Anti-GSK3 β (phospho Ser9) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00791-1-gsk3b-primary-antibodies-wb-testing-1.jpgAnti-GSK3 β Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GSK3 β (phospho Ser9) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Mouse skin Lane 3: HEK293 treated with serum in 30 minutes Predicted band size: 46kDa Observed band size: 46kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-erg-rabbit-monoclonal-antibody-m00793-3-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00793-3-erg-primary-antibodies-ihc-testing-1.jpgAnti-ERG Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-ERG rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00793-3-erg-primary-antibodies-wb-testing-1.jpgAnti-ERG Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ERG antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse thymus Lane 2: Rat thymus Lane 3: HeLa Lane 4: Jurkat Predicted band size: 55kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pkc-d-rabbit-monoclonal-antibody-m00822-3-boster.html2026-03-16T05:09:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00822-3-prkcd-primary-antibodies-ihc-testing-1.jpgAnti-PKC δ Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-PKC δ rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00822-3-prkcd-primary-antibodies-ihc-testing-2.jpgAnti-PKC δ Rabbit Monoclonal AntibodyHuman spleen was stained with anti-PKC δ rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00822-3-prkcd-primary-antibodies-wb-testing-1.jpgAnti-PKC δ Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PKC δ antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: C6 Lane 3: 3T3-L1 Lane 4: HeLa Predicted band size: 78kDa Observed band size: 78kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lactate-dehydrogenase-isoenzyme-v-rabbit-monoclonal-antibody-m00825-3-boster.html2026-03-16T05:09:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00825-3-ldha-primary-antibodies-ihc-testing-1.jpgAnti-Lactate Dehydrogenase Isoenzyme V Rabbit Monoclonal AntibodyRat heart was stained with Anti-Lactate Dehydrogenase Isoenzyme V rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00825-3-ldha-primary-antibodies-ihc-testing-2.jpgAnti-Lactate Dehydrogenase Isoenzyme V Rabbit Monoclonal AntibodyMouse heart was stained with Anti-Lactate Dehydrogenase Isoenzyme V rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00825-3-ldha-primary-antibodies-wb-testing-1.jpgAnti-Lactate Dehydrogenase Isoenzyme V Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Lactate Dehydrogenase Isoenzyme V antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: HeLa Predicted band size: 36kDa Observed band size: 36kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lactate-dehydrogenase-rabbit-monoclonal-antibody-m00825-4-boster.html2026-03-16T05:09:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00825-4-ldha-primary-antibodies-ihc-testing-1.jpgAnti-Lactate Dehydrogenase Rabbit Monoclonal AntibodyRat liver was stained with anti-Lactate Dehydrogenase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00825-4-ldha-primary-antibodies-ihc-testing-2.jpgAnti-Lactate Dehydrogenase Rabbit Monoclonal AntibodyHuman liver was stained with anti-Lactate Dehydrogenase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00825-4-ldha-primary-antibodies-ihc-testing-3.jpgAnti-Lactate Dehydrogenase Rabbit Monoclonal AntibodyHuman skeletal muscle was stained with anti-Lactate Dehydrogenase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00825-4-ldha-primary-antibodies-ihc-testing-4.jpgAnti-Lactate Dehydrogenase Rabbit Monoclonal AntibodyMouse liver was stained with anti-Lactate Dehydrogenase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00825-4-ldha-primary-antibodies-wb-testing-1.jpgAnti-Lactate Dehydrogenase Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Lactate Dehydrogenase antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Jurkat Lane 3: Mouse brain Lane 4: Rat brain Predicted band size: 37kDa Observed band size: 37kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lrp1-rabbit-monoclonal-antibody-m00829-1-boster.html2026-03-16T05:09:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00829-1-lrp1-primary-antibodies-ihc-testing-1.jpgAnti-LRP1 Rabbit Monoclonal AntibodyMouse colon was stained with anti-LRP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00829-1-lrp1-primary-antibodies-ihc-testing-2.jpgAnti-LRP1 Rabbit Monoclonal AntibodyRat colon was stained with anti-LRP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00829-1-lrp1-primary-antibodies-ihc-testing-3.jpgAnti-LRP1 Rabbit Monoclonal AntibodyHuman liver was stained with anti-LRP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00829-1-lrp1-primary-antibodies-wb-testing-1.jpgAnti-LRP1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LRP1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: Mouse brain Lane 3: Rat brain Predicted band size: 85kDa Observed band size: 85kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-zo1-rabbit-monoclonal-antibody-m00860-2-boster.html2026-03-16T05:09:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00860-2-tjp1-primary-antibodies-ihc-testing-1.jpgAnti-ZO1 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-ZO1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00860-2-tjp1-primary-antibodies-ihc-testing-2.jpgAnti-ZO1 Rabbit Monoclonal AntibodyRat spleen was stained with anti-ZO1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00860-2-tjp1-primary-antibodies-ihc-testing-3.jpgAnti-ZO1 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-ZO1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00860-2-tjp1-primary-antibodies-wb-testing-4.jpgAnti-ZO1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-ZO1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C2C12 Lane 2: A431 Predicted band size: 195kDa Observed band size: 220kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00860-2-tjp1-primary-antibodies-wb-testing-5.jpgAnti-ZO1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ZO1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: L6 Predicted band size: 195kDa Observed band size: 220kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ctgf-rabbit-monoclonal-antibody-m00886-1-boster.html2026-03-16T05:09:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00886-1-ctgf-primary-antibodies-wb-testing-1.jpgAnti-CTGF Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CTGF antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-14 Lane 2: HeLa Lane 3: L6 Predicted band size: 38kDa Observed band size: 38kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-calreticulin-rabbit-monoclonal-antibody-m00894-3-boster.html2026-03-16T05:09:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00894-3-calr-primary-antibodies-ihc-testing-1.jpgAnti-Calreticulin Rabbit Monoclonal AntibodyMouse liver was stained with anti-Calreticulin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00894-3-calr-primary-antibodies-ihc-testing-2.jpgAnti-Calreticulin Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Calreticulin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00894-3-calr-primary-antibodies-ihc-testing-3.jpgAnti-Calreticulin Rabbit Monoclonal AntibodyHuman stomach was stained with anti-Calreticulin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00894-3-calr-primary-antibodies-wb-testing-1.jpgAnti-Calreticulin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Calreticulin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: HepG2 Predicted band size: 48kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ask1-rabbit-monoclonal-antibody-m00929-1-boster.html2026-03-16T05:09:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00929-1-map3k5-primary-antibodies-ihc-testing-1.jpgAnti-ASK1 Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-ASK1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00929-1-map3k5-primary-antibodies-wb-testing-1.jpgAnti-ASK1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ASK1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SiHa Predicted band size: 155kDa Observed band size: 155kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fatty-acid-synthase-rabbit-monoclonal-antibody-m00941-2-boster.html2026-03-16T05:09:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00941-2-fasn-primary-antibodies-ihc-testing-1.jpgAnti-Fatty Acid Synthase Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-Fatty Acid Synthase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00941-2-fasn-primary-antibodies-ihc-testing-2.jpgAnti-Fatty Acid Synthase Rabbit Monoclonal AntibodyHuman lung was stained with anti-Fatty Acid Synthase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00941-2-fasn-primary-antibodies-ihc-testing-3.jpgAnti-Fatty Acid Synthase Rabbit Monoclonal AntibodyMouse liver was stained with anti-Fatty Acid Synthase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00941-2-fasn-primary-antibodies-ihc-testing-4.jpgAnti-Fatty Acid Synthase Rabbit Monoclonal AntibodyRat liver was stained with anti-Fatty Acid Synthase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00941-2-fasn-primary-antibodies-wb-testing-5.jpgAnti-Fatty Acid Synthase Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Fatty Acid Synthase antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: L6 Predicted band size: 273kDa Observed band size: 273kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00941-2-fasn-primary-antibodies-wb-testing-6.jpgAnti-Fatty Acid Synthase Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Fatty Acid Synthase antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Predicted band size: 273kDa Observed band size: 273kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pax-8-rabbit-monoclonal-antibody-m00943-4-boster.html2026-03-16T05:09:13+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hsp70-rabbit-monoclonal-antibody-m00949-3-boster.html2026-03-16T05:09:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00949-3-hsp70-primary-antibodies-ihc-testing-1.jpgAnti-Hsp70 Rabbit Monoclonal AntibodyMouse brain was stained with Anti-Hsp70 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00949-3-hsp70-primary-antibodies-ihc-testing-2.jpgAnti-Hsp70 Rabbit Monoclonal AntibodyHuman prostate was stained with Anti-Hsp70 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00949-3-hsp70-primary-antibodies-wb-testing-1.jpgAnti-Hsp70 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Hsp70 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A172 Lane 2: HeLa Lane 3: Mouse brain Lane 4: Rat brain Predicted band size: 70kDa Observed band size: 70kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-optineurin-rabbit-monoclonal-antibody-m00952-2-boster.html2026-03-16T05:09:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00952-2-optn-primary-antibodies-ihc-testing-1.jpgAnti-Optineurin Rabbit Monoclonal AntibodyMouse brain was stained with Anti-Optineurin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00952-2-optn-primary-antibodies-ihc-testing-2.jpgAnti-Optineurin Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with Anti-Optineurin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00952-2-optn-primary-antibodies-ihc-testing-3.jpgAnti-Optineurin Rabbit Monoclonal AntibodyHuman brain was stained with Anti-Optineurin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00952-2-optn-primary-antibodies-wb-testing-4.jpgAnti-Optineurin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Optineurin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Predicted band size: 63kDa Observed band size: 74kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00952-2-optn-primary-antibodies-wb-testing-5.jpgAnti-Optineurin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Optineurin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HepG2 Lane 3: Mouse liver Lane 4: Rat liver Predicted band size: 63kDa Observed band size: 70kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-grp78-bip-rabbit-monoclonal-antibody-m00955-4-boster.html2026-03-16T05:09:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00955-4-hspa5-primary-antibodies-ihc-testing-1.jpgAnti-GRP78/BiP Rabbit Monoclonal AntibodyRat brain was stained with anti-GRP78/BiP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00955-4-hspa5-primary-antibodies-ihc-testing-2.jpgAnti-GRP78/BiP Rabbit Monoclonal AntibodyHuman brain was stained with anti-GRP78/BiP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00955-4-hspa5-primary-antibodies-ihc-testing-3.jpgAnti-GRP78/BiP Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-GRP78/BiP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00955-4-hspa5-primary-antibodies-ihc-testing-4.jpgAnti-GRP78/BiP Rabbit Monoclonal AntibodyMouse brain was stained with anti-GRP78/BiP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00955-4-hspa5-primary-antibodies-wb-testing-1.jpgAnti-GRP78/BiP Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GRP78/BiP antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: RAW264.7 Lane 3: HepG2 Lane 4: HeLa Predicted band size: 72kDa Observed band size: 78kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-4e-bp1-rabbit-monoclonal-antibody-m00968-1-boster.html2026-03-16T05:09:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00968-1-eif4ebp1-primary-antibodies-ihc-testing-1.jpgAnti-4E-BP1 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-4E-BP1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00968-1-eif4ebp1-primary-antibodies-wb-testing-1.jpgAnti-4E-BP1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-4E-BP1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: C6 Lane 3: NIH-3T3 Lane 4: HEK293 Predicted band size: 13kDa Observed band size: 17kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lrp6-rabbit-monoclonal-antibody-m00970-1-boster.html2026-03-16T05:09:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00970-1-lrp6-primary-antibodies-ihc-testing-1.jpgAnti-LRP6 Rabbit Monoclonal AntibodyRatbrain was stained with anti-LRP6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00970-1-lrp6-primary-antibodies-ihc-testing-2.jpgAnti-LRP6 Rabbit Monoclonal AntibodyHumanbrain was stained with anti-LRP6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00970-1-lrp6-primary-antibodies-wb-testing-1.jpgAnti-LRP6 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-LRP6 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Jurkat Lane 3: HEK293 Predicted band size: 180kDa Observed band size: 210kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hdac4-rabbit-monoclonal-antibody-m00971-2-boster.html2026-03-16T05:09:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00971-2-hdac4-primary-antibodies-ihc-testing-1.jpgAnti-HDAC4 Rabbit Monoclonal AntibodyMouse brain was stained with anti-HDAC4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00971-2-hdac4-primary-antibodies-ihc-testing-2.jpgAnti-HDAC4 Rabbit Monoclonal AntibodyRat brain was stained with anti-HDAC4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00971-2-hdac4-primary-antibodies-wb-testing-1.jpgAnti-HDAC4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-HDAC4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HeLa Lane 3: NIH-3T3 Lane 4: Rat womb Predicted band size: 119kDa Observed band size: 140kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-s100b-rabbit-monoclonal-antibody-m00979-4-boster.html2026-03-16T05:09:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00979-4-s100b-primary-antibodies-ihc-testing-1.jpgAnti-S100B Rabbit Monoclonal AntibodyHuman colon was stained with anti-S100B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00979-4-s100b-primary-antibodies-ihc-testing-2.jpgAnti-S100B Rabbit Monoclonal AntibodyHuman liver was stained with anti-S100B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00979-4-s100b-primary-antibodies-ihc-testing-3.jpgAnti-S100B Rabbit Monoclonal AntibodyMouse colon was stained with anti-S100B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00979-4-s100b-primary-antibodies-ihc-testing-4.jpgAnti-S100B Rabbit Monoclonal AntibodyRat colon was stained with anti-S100B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00979-4-s100b-primary-antibodies-wb-testing-1.jpgAnti-S100B Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-S100B antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SK-MEL-28 Lane 2: Rat brain Lane 3: Mouse brain Predicted band size: 11kDa Observed band size: 11kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-twist1-rabbit-monoclonal-antibody-m00980-1-boster.html2026-03-16T05:09:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00980-1-twist1-primary-antibodies-wb-testing-1.jpgAnti-TWIST1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TWIST1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat spleen Lane 2: SH-SY5Y Lane 3: 3T3-L1 Lane 4: U-87 MG Predicted band size: 21kDa Observed band size: 26kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ampk-alpha1-rabbit-monoclonal-antibody-m00994-2-boster.html2026-03-16T05:09:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00994-2-prkaa1-primary-antibodies-ihc-testing-1.jpgAnti-AMPK α1 Rabbit Monoclonal AntibodyHuman cervical carcinoma was stained with Anti-AMPK α1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00994-2-prkaa1-primary-antibodies-wb-testing-1.jpgAnti-AMPK α1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-AMPK α1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: NIH3T3 Predicted band size: 64kDa Observed band size: 64kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tdp43-rabbit-monoclonal-antibody-m01001-3-boster.html2026-03-16T05:09:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01001-3-tardbp-primary-antibodies-ihc-testing-1.jpgAnti-TDP43 Rabbit Monoclonal AntibodyRat brain was stained with anti-TDP43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01001-3-tardbp-primary-antibodies-ihc-testing-2.jpgAnti-TDP43 Rabbit Monoclonal AntibodyHuman brain was stained with anti-TDP43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01001-3-tardbp-primary-antibodies-ihc-testing-3.jpgAnti-TDP43 Rabbit Monoclonal AntibodyHuman pancreas was stained with anti-TDP43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01001-3-tardbp-primary-antibodies-ihc-testing-4.jpgAnti-TDP43 Rabbit Monoclonal AntibodyMouse brain was stained with anti-TDP43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01001-3-tardbp-primary-antibodies-wb-testing-1.jpgAnti-TDP43 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TDP43 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: K562 Lane 3: C6 Lane 4: 3T3-L1 Predicted band size: 45kDa Observed band size: 45kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pgp9-5-rabbit-monoclonal-antibody-m01018-8-boster.html2026-03-16T05:09:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01018-8-uchl1-primary-antibodies-ihc-testing-1.jpgAnti-PGP9.5 Rabbit Monoclonal AntibodyMouse kidney was stained with anti-PGP9.5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01018-8-uchl1-primary-antibodies-ihc-testing-2.jpgAnti-PGP9.5 Rabbit Monoclonal AntibodyRat kidney was stained with anti-PGP9.5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01018-8-uchl1-primary-antibodies-ihc-testing-3.jpgAnti-PGP9.5 Rabbit Monoclonal AntibodyHuman appendix was stained with anti-PGP9.5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01018-8-uchl1-primary-antibodies-ihc-testing-4.jpgAnti-PGP9.5 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-PGP9.5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01018-8-uchl1-primary-antibodies-wb-testing-1.jpgAnti-PGP9.5 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PGP9.5 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: U-87 MG Lane 3: C6 Lane 4: Mouse brain Predicted band size: 25kDa Observed band size: 25kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pms2-rabbit-monoclonal-antibody-m01028-3-boster.html2026-03-16T05:09:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01028-3-pms2-primary-antibodies-ihc-testing-1.jpgAnti-PMS2 Rabbit Monoclonal AntibodyHuman rectal carcinoma tissue was stained with anti-PMS2 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01028-3-pms2-primary-antibodies-wb-testing-1.jpgAnti-PMS2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PMS2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: A549 Predicted band size: 96kDa Observed band size: 110kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rpb1-rabbit-monoclonal-antibody-m01029-2-boster.html2026-03-16T05:09:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01029-2-polr2a-primary-antibodies-ihc-testing-1.jpgAnti-Rpb1 Rabbit Monoclonal AntibodyHuman colon was stained with anti-Rpb1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01029-2-polr2a-primary-antibodies-wb-testing-1.jpgAnti-Rpb1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Rpb1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: Mouse lung Lane 3: Rat lung Predicted band size: 192kDa Observed band size: 250kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01029-2-polr2a-primary-antibodies-wb-testing-2.jpgAnti-Rpb1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Rpb1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Predicted band size: 192kDa Observed band size: 250kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-paxillin-rabbit-monoclonal-antibody-m01033-3-boster.html2026-03-16T05:09:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01033-3-pxn-primary-antibodies-ihc-testing-1.jpgAnti-Paxillin Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Paxillin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01033-3-pxn-primary-antibodies-ihc-testing-2.jpgAnti-Paxillin Rabbit Monoclonal AntibodyMouse kidney was stained with anti-Paxillin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01033-3-pxn-primary-antibodies-ihc-testing-3.jpgAnti-Paxillin Rabbit Monoclonal AntibodyRat kidney was stained with anti-Paxillin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01033-3-pxn-primary-antibodies-wb-testing-1.jpgAnti-Paxillin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Paxillin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: RAW264.7 Predicted band size: 68kDa Observed band size: 68kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lats1-rabbit-monoclonal-antibody-m01051-1-boster.html2026-03-16T05:09:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01051-1-lats1-primary-antibodies-wb-testing-1.jpgAnti-LATS1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-LATS1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C2C12 Lane 2: PC-12 Predicted band size: 127kDa Observed band size: 140kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01051-1-lats1-primary-antibodies-wb-testing-2.jpgAnti-LATS1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LATS1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HT-1080 Predicted band size: 127kDa Observed band size: 140kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p90rsk-rabbit-monoclonal-antibody-m01058-3-boster.html2026-03-16T05:09:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01058-3-rps6ka1-primary-antibodies-ihc-testing-1.jpgAnti-p90RSK Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-p90RSK (phospho Thr359/Ser363) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01058-3-rps6ka1-primary-antibodies-ihc-testing-2.jpgAnti-p90RSK Rabbit Monoclonal AntibodyMouse colon was stained with anti-p90RSK (phospho Thr359/Ser363) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01058-3-rps6ka1-primary-antibodies-ihc-testing-3.jpgAnti-p90RSK Rabbit Monoclonal AntibodyRat colon was stained with anti-p90RSK (phospho Thr359/Ser363) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01058-3-rps6ka1-primary-antibodies-wb-testing-4.jpgAnti-p90RSK Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p90RSK (phospho Thr359/Ser363) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: NIH-3T3 Lane 3: PC-12 Predicted band size: 83kDa Observed band size: 83kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01058-3-rps6ka1-primary-antibodies-wb-testing-5.jpgAnti-p90RSK Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p90RSK (phospho Thr359/Ser363) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: HeLa was tearted with TPA(200nM) for 30 minutes Predicted band size: 83kDa Observed band size: 83kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p90rsk-rabbit-monoclonal-antibody-m01058-4-boster.html2026-03-16T05:09:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01058-4-rps6ka1-primary-antibodies-ihc-testing-1.jpgAnti-p90RSK Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-p90RSK (phospho Ser380) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01058-4-rps6ka1-primary-antibodies-wb-testing-1.jpgAnti-p90RSK Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p90RSK (phospho Ser380) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: A431 Lane 3: NIH-3T3 Lane 4: Rat womb Predicted band size: 83kDa Observed band size: 83kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p90rsk-rabbit-monoclonal-antibody-m01058-5-boster.html2026-03-16T05:09:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01058-5-rps6ka1-primary-antibodies-ihc-testing-1.jpgAnti-p90RSK Rabbit Monoclonal AntibodyHuman colon was stained with anti-p90RSK rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01058-5-rps6ka1-primary-antibodies-ihc-testing-2.jpgAnti-p90RSK Rabbit Monoclonal AntibodyRat colon was stained with anti-p90RSK rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01058-5-rps6ka1-primary-antibodies-ihc-testing-3.jpgAnti-p90RSK Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-p90RSK rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01058-5-rps6ka1-primary-antibodies-wb-testing-1.jpgAnti-p90RSK Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p90RSK antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat womb Lane 2: A431 Lane 3: HeLa Predicted band size: 83kDa Observed band size: 83kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chk1-rabbit-monoclonal-antibody-m01060-3-boster.html2026-03-16T05:09:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01060-3-chek1-primary-antibodies-wb-testing-1.jpgAnti-Chk1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Chk1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: 3T3-L1 Lane 3: HeLa Lane 4: PC-12 Predicted band size: 54kDa Observed band size: 54kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sirt3-rabbit-monoclonal-antibody-m01061-1-boster.html2026-03-16T05:09:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01061-1-sirt3-primary-antibodies-ihc-testing-1.jpgAnti-SIRT3 Rabbit Monoclonal AntibodyRat liver was stained with anti-SIRT3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01061-1-sirt3-primary-antibodies-ihc-testing-2.jpgAnti-SIRT3 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-SIRT3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01061-1-sirt3-primary-antibodies-ihc-testing-3.jpgAnti-SIRT3 Rabbit Monoclonal AntibodyHuman liver was stained with anti-SIRT3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01061-1-sirt3-primary-antibodies-ihc-testing-4.jpgAnti-SIRT3 Rabbit Monoclonal AntibodyMouse liver was stained with anti-SIRT3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01061-1-sirt3-primary-antibodies-wb-testing-1.jpgAnti-SIRT3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SIRT3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-14 Lane 2: HeLa Lane 3: Jurkat Predicted band size: 44kDa Observed band size: 28kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alpha-smooth-muscle-actin-rabbit-monoclonal-antibody-m01072-5-boster.html2026-03-16T05:09:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01072-5-acta2-primary-antibodies-ihc-testing-1.jpgAnti-α smooth muscle actin Rabbit Monoclonal AntibodyHuman colon was stained with anti-α smooth muscle actin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01072-5-acta2-primary-antibodies-ihc-testing-2.jpgAnti-α smooth muscle actin Rabbit Monoclonal AntibodyHuman stomach was stained with anti-α smooth muscle actin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01072-5-acta2-primary-antibodies-wb-testing-1.jpgAnti-α smooth muscle actin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-α smooth muscle Actin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: NIH-3T3 Lane 3: Rat lung Lane 4: C6 Predicted band size: 42kDa Observed band size: 42kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-actin-pan-rabbit-monoclonal-antibody-m01072-6-boster.html2026-03-16T05:09:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01072-6-actin-primary-antibodies-ihc-testing-1.jpgAnti-Actin pan Rabbit Monoclonal AntibodyMouse heart was stained with Anti-Actin pan rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01072-6-actin-primary-antibodies-ihc-testing-2.jpgAnti-Actin pan Rabbit Monoclonal AntibodyRat heart was stained with Anti-Actin pan rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01072-6-actin-primary-antibodies-ihc-testing-3.jpgAnti-Actin pan Rabbit Monoclonal AntibodyHuman skeletal muscle was stained with Anti-Actin pan rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01072-6-actin-primary-antibodies-wb-testing-1.jpgAnti-Actin pan Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Actin pan antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: HeLa Lane 3: PC-12 Lane 4: RAW264.7 Predicted band size: 42kDa Observed band size: 42kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd63-rabbit-monoclonal-antibody-m01080-4-boster.html2026-03-16T05:09:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01080-4-cd63-primary-antibodies-ihc-testing-1.jpgAnti-CD63 Rabbit Monoclonal AntibodyMouse placenta was stained with anti-CD63 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01080-4-cd63-primary-antibodies-wb-testing-1.jpgAnti-CD63 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD63 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Predicted band size: 26kDa Observed band size: 30-65kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-carbonic-anhydrase-9-rabbit-monoclonal-antibody-m01083-5-boster.html2026-03-16T05:09:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01083-5-ca9-primary-antibodies-ihc-testing-1.jpgAnti-Carbonic Anhydrase 9 Rabbit Monoclonal AntibodyHuman tonsil was stained with Anti-Carbonic Anhydrase 9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01083-5-ca9-primary-antibodies-ihc-testing-2.jpgAnti-Carbonic Anhydrase 9 Rabbit Monoclonal AntibodyRat stomach was stained with Anti-Carbonic Anhydrase 9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01083-5-ca9-primary-antibodies-ihc-testing-3.jpgAnti-Carbonic Anhydrase 9 Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with Anti-Carbonic Anhydrase 9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01083-5-ca9-primary-antibodies-ihc-testing-4.jpgAnti-Carbonic Anhydrase 9 Rabbit Monoclonal AntibodyHuman stomach was stained with Anti-Carbonic Anhydrase 9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01083-5-ca9-primary-antibodies-wb-testing-1.jpgAnti-Carbonic Anhydrase 9 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Carbonic Anhydrase 9 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-12 Lane 2: U-14 Lane 3: Rat womb Predicted band size: 49kDa Observed band size: 49kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stat5a-b-rabbit-monoclonal-antibody-m01087-3-boster.html2026-03-16T05:09:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01087-3-stat5a-primary-antibodies-ihc-testing-1.jpgAnti-STAT5A/B Rabbit Monoclonal AntibodyHuman spleen was stained with anti-STAT5A/B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01087-3-stat5a-primary-antibodies-ihc-testing-2.jpgAnti-STAT5A/B Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-STAT5A/B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01087-3-stat5a-primary-antibodies-wb-testing-1.jpgAnti-STAT5A/B Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-STAT5A/B antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HeLa Lane 3: 3T3-L1 Lane 4: C6 Predicted band size: 91kDa Observed band size: 100kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stat5a-rabbit-monoclonal-antibody-m01087-4-boster.html2026-03-16T05:09:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01087-4-stat5a-primary-antibodies-ihc-testing-1.jpgAnti-STAT5A Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-STAT5A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01087-4-stat5a-primary-antibodies-wb-testing-1.jpgAnti-STAT5A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-STAT5A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: Mouse thymus Predicted band size: 91kDa Observed band size: 91kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sdhb-rabbit-monoclonal-antibody-m01090-5-boster.html2026-03-16T05:09:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-5-sdhb-primary-antibodies-ihc-testing-1.jpgAnti-SDHB Rabbit Monoclonal AntibodyRat kidney was stained with anti-SDHB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-5-sdhb-primary-antibodies-ihc-testing-2.jpgAnti-SDHB Rabbit Monoclonal AntibodyHuman kidney was stained with anti-SDHB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-5-sdhb-primary-antibodies-ihc-testing-3.jpgAnti-SDHB Rabbit Monoclonal AntibodyHuman liver was stained with anti-SDHB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-5-sdhb-primary-antibodies-ihc-testing-4.jpgAnti-SDHB Rabbit Monoclonal AntibodyMouse kidney was stained with anti-SDHB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01090-5-sdhb-primary-antibodies-wb-testing-1.jpgAnti-SDHB Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SDHB antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: Mouse spleen Lane 3: Rat thymus Lane 4: HepG2 Predicted band size: 31kDa Observed band size: 31kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-arginase-1-rabbit-monoclonal-antibody-m01106-5-boster.html2026-03-16T05:09:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01106-5-arg1-primary-antibodies-ihc-testing-1.jpgAnti-Arginase-1 Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-Arginase-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01106-5-arg1-primary-antibodies-ihc-testing-2.jpgAnti-Arginase-1 Rabbit Monoclonal AntibodyHuman liver was stained with anti-Arginase-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01106-5-arg1-primary-antibodies-ihc-testing-3.jpgAnti-Arginase-1 Rabbit Monoclonal AntibodyMouse liver was stained with anti-Arginase-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01106-5-arg1-primary-antibodies-ihc-testing-4.jpgAnti-Arginase-1 Rabbit Monoclonal AntibodyRat liver was stained with anti-Arginase-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01106-5-arg1-primary-antibodies-wb-testing-1.jpgAnti-Arginase-1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Arginase-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: huh-7 Lane 2: Rat liver Predicted band size: 35kDa Observed band size: 35kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mttfa-rabbit-monoclonal-antibody-m01119-2-boster.html2026-03-16T05:09:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01119-2-tfam-primary-antibodies-ihc-testing-1.jpgAnti-mtTFA Rabbit Monoclonal AntibodyHuman thyroid carcinoma was stained with anti-mtTFA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01119-2-tfam-primary-antibodies-ihc-testing-2.jpgAnti-mtTFA Rabbit Monoclonal AntibodyHuman kidney was stained with anti-mtTFA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01119-2-tfam-primary-antibodies-wb-testing-1.jpgAnti-mtTFA Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-mtTFA antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HeLa Predicted band size: 29kDa Observed band size: 24kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-podoplanin-rabbit-monoclonal-antibody-m01124-4-boster.html2026-03-16T05:09:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01124-4-pdpn-primary-antibodies-ihc-testing-1.jpgAnti-Podoplanin Rabbit Monoclonal AntibodyMosue lung was stained with anti-Podoplanin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01124-4-pdpn-primary-antibodies-ihc-testing-2.jpgAnti-Podoplanin Rabbit Monoclonal AntibodyRat lung was stained with anti-Podoplanin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01124-4-pdpn-primary-antibodies-ihc-testing-3.jpgAnti-Podoplanin Rabbit Monoclonal AntibodyHuman lung squamous carcinoma was stained with anti-Podoplanin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01124-4-pdpn-primary-antibodies-wb-testing-1.jpgAnti-Podoplanin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Podoplanin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse brain Lane 2: Rat brain Predicted band size: 17kDa Observed band size: 36kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cardiac-troponin-t-rabbit-monoclonal-antibody-m01154-4-boster.html2026-03-16T05:09:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01154-4-tnnt2-primary-antibodies-ihc-testing-1.jpgAnti-Cardiac Troponin T Rabbit Monoclonal AntibodyRat cardiac muscle was stained with anti-Cardiac Troponin T rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01154-4-tnnt2-primary-antibodies-ihc-testing-2.jpgAnti-Cardiac Troponin T Rabbit Monoclonal AntibodyMouse cardiac muscle was stained with anti-Cardiac Troponin T rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01154-4-tnnt2-primary-antibodies-wb-testing-1.jpgAnti-Cardiac Troponin T Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cardiac Troponin T antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat heart Predicted band size: 36kDa Observed band size: 36kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bak-rabbit-monoclonal-antibody-m01163-2-boster.html2026-03-16T05:09:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01163-2-bak1-primary-antibodies-ihc-testing-1.jpgAnti-Bak Rabbit Monoclonal AntibodyHuman colon was stained with anti-Bak rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01163-2-bak1-primary-antibodies-wb-testing-1.jpgAnti-Bak Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Bak antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HeLa Lane 3: A20 Predicted band size: 23kDa Observed band size: 23kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vdac1-rabbit-monoclonal-antibody-m01168-1-boster.html2026-03-16T05:09:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01168-1-vdac1-primary-antibodies-ihc-testing-1.jpgAnti-VDAC1 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-VDAC1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01168-1-vdac1-primary-antibodies-ihc-testing-2.jpgAnti-VDAC1 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-VDAC1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01168-1-vdac1-primary-antibodies-ihc-testing-3.jpgAnti-VDAC1 Rabbit Monoclonal AntibodyMouse kidney was stained with anti-VDAC1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01168-1-vdac1-primary-antibodies-ihc-testing-4.jpgAnti-VDAC1 Rabbit Monoclonal AntibodyRat kidney was stained with anti-VDAC1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01168-1-vdac1-primary-antibodies-wb-testing-1.jpgAnti-VDAC1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-VDAC1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Mouse brain Lane 3: Rat kidney Predicted band size: 31kDa Observed band size: 33kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pkm-rabbit-monoclonal-antibody-m01173-2-boster.html2026-03-16T05:09:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01173-2-pkm-primary-antibodies-ihc-testing-1.jpgAnti-PKM Rabbit Monoclonal AntibodyRat lung was stained with Anti-PKM rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01173-2-pkm-primary-antibodies-ihc-testing-2.jpgAnti-PKM Rabbit Monoclonal AntibodyMouse lung was stained with Anti-PKM rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01173-2-pkm-primary-antibodies-ihc-testing-3.jpgAnti-PKM Rabbit Monoclonal AntibodyHuman kidney was stained with Anti-PKM rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01173-2-pkm-primary-antibodies-ihc-testing-4.jpgAnti-PKM Rabbit Monoclonal AntibodyHuman lung was stained with Anti-PKM rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01173-2-pkm-primary-antibodies-wb-testing-1.jpgAnti-PKM Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PKM antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: NIH3T3 Lane 3: C6 Predicted band size: 57kDa Observed band size: 57kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prohibitin-rabbit-monoclonal-antibody-m01178-1-boster.html2026-03-16T05:09:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01178-1-phb-primary-antibodies-ihc-testing-1.jpgAnti-Prohibitin Rabbit Monoclonal AntibodyMouse kidney was stained with Anti-Prohibitin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01178-1-phb-primary-antibodies-ihc-testing-2.jpgAnti-Prohibitin Rabbit Monoclonal AntibodyRat kidney was stained with Anti-Prohibitin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01178-1-phb-primary-antibodies-ihc-testing-3.jpgAnti-Prohibitin Rabbit Monoclonal AntibodyHuman kidney was stained with Anti-Prohibitin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01178-1-phb-primary-antibodies-ihc-testing-4.jpgAnti-Prohibitin Rabbit Monoclonal AntibodyHuman liver was stained with Anti-Prohibitin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01178-1-phb-primary-antibodies-wb-testing-1.jpgAnti-Prohibitin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Prohibitin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: C6 Lane 3: Mouse spleen Predicted band size: 30kDa Observed band size: 30kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd36-rabbit-monoclonal-antibody-m01189-3-boster.html2026-03-16T05:09:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01189-3-cd36-primary-antibodies-ihc-testing-1.jpgAnti-CD36 Rabbit Monoclonal AntibodyRat spleen was stained with anti-CD36 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01189-3-cd36-primary-antibodies-ihc-testing-2.jpgAnti-CD36 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-CD36 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01189-3-cd36-primary-antibodies-ihc-testing-3.jpgAnti-CD36 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-CD36 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01189-3-cd36-primary-antibodies-wb-testing-1.jpgAnti-CD36 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD36 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse spleen Lane 2: Rat spleen Lane 3: U937 Predicted band size: 53kDa Observed band size: 90kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hla-dr-rabbit-monoclonal-antibody-m01195-6-boster.html2026-03-16T05:09:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-6-hla-dra-primary-antibodies-ihc-testing-1.jpgAnti-HLA-DR Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-HLA-DR rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01195-6-hla-dra-primary-antibodies-wb-testing-1.jpgAnti-HLA-DR Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HLA-DR antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Daudi Lane 2: Rat spleen Predicted band size: 29kDa Observed band size: 37kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd9-rabbit-monoclonal-antibody-m01202-2-boster.html2026-03-16T05:09:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01202-2-cd9-primary-antibodies-ihc-testing-1.jpgAnti-CD9 Rabbit Monoclonal AntibodyHuman bladder carcinoma was stained with anti-CD9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01202-2-cd9-primary-antibodies-ihc-testing-2.jpgAnti-CD9 Rabbit Monoclonal AntibodyHuman cervical carcinoma was stained with anti-CD9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01202-2-cd9-primary-antibodies-wb-testing-1.jpgAnti-CD9 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD9 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat womb Lane 2: HeLa Lane 3: HCT-116 Lane 4: U-14 Predicted band size: 25kDa Observed band size: 25kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vinculin-rabbit-monoclonal-antibody-m01207-3-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01207-3-vcl-primary-antibodies-ihc-testing-1.jpgAnti-Vinculin Rabbit Monoclonal AntibodyHuman colon was stained with anti-Vinculin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01207-3-vcl-primary-antibodies-wb-testing-1.jpgAnti-Vinculin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Vinculin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: C6 Lane 3: K562 Predicted band size: 124kDa Observed band size: 124kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-muc2-rabbit-monoclonal-antibody-m01212-3-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01212-3-muc2-primary-antibodies-ihc-testing-1.jpgAnti-MUC2 Rabbit Monoclonal AntibodyHuman colon was stained with anti-MUC2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01212-3-muc2-primary-antibodies-ihc-testing-2.jpgAnti-MUC2 Rabbit Monoclonal AntibodyMouse colon was stained with anti-MUC2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01212-3-muc2-primary-antibodies-wb-testing-1.jpgAnti-MUC2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MUC2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse colon Predicted band size: 551kDa Observed band size: 140-170kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nfkb-p100-rabbit-monoclonal-antibody-m01228-2-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01228-2-nfkb2-primary-antibodies-wb-testing-1.jpgAnti-NFkB-p100 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NFkB p100 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: MCF7 Lane 3: 4T1 Lane 4: Rat womb Predicted band size: 97kDa Observed band size: 120kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tsg101-rabbit-monoclonal-antibody-m01233-2-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01233-2-tsg101-primary-antibodies-wb-testing-1.jpgAnti-TSG101 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TSG101 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: K562 Lane 3: NIH-3T3 Lane 4: PC-12 Predicted band size: 44kDa Observed band size: 44kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p57kip2-rabbit-monoclonal-antibody-m01244-3-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01244-3-cdkn1c-primary-antibodies-ihc-testing-1.jpgAnti-p57kip2 Rabbit Monoclonal AntibodyHuman kidney tissue was stained with anti-p57kip2 rabbit Antibody https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-albumin-rabbit-monoclonal-antibody-m01245-4-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01245-4-alb-primary-antibodies-ihc-testing-1.jpgAnti-Albumin Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-Albumin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01245-4-alb-primary-antibodies-ihc-testing-2.jpgAnti-Albumin Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Albumin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01245-4-alb-primary-antibodies-ihc-testing-3.jpgAnti-Albumin Rabbit Monoclonal AntibodyRat kidney was stained with anti-Albumin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01245-4-alb-primary-antibodies-wb-testing-1.jpgAnti-Albumin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Albumin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: huh-7 Lane 2: HepG2 Lane 3: HEK293 Lane 4: Mouse liver Lane 5: Rat liver Predicted band size: 69kDa Observed band size: 69kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ocln-rabbit-monoclonal-antibody-m01246-1-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01246-1-ocln-primary-antibodies-ihc-testing-1.jpgAnti-OCLN Rabbit Monoclonal AntibodyRat kidney was stained with anti-OCLN rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01246-1-ocln-primary-antibodies-ihc-testing-2.jpgAnti-OCLN Rabbit Monoclonal AntibodyHuman kidney was stained with anti-OCLN rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01246-1-ocln-primary-antibodies-ihc-testing-3.jpgAnti-OCLN Rabbit Monoclonal AntibodyMouse kidney was stained with anti-OCLN rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01246-1-ocln-primary-antibodies-wb-testing-1.jpgAnti-OCLN Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-OCLN antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: Mouse liver Lane 3: Rat liver Predicted band size: 59kDa Observed band size: 65kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chromogranin-a-rabbit-monoclonal-antibody-m01256-3-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01256-3-chga-primary-antibodies-ihc-testing-1.jpgAnti-Chromogranin A Rabbit Monoclonal AntibodyRat pancreas was stained with anti-Chromogranin A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01256-3-chga-primary-antibodies-ihc-testing-2.jpgAnti-Chromogranin A Rabbit Monoclonal AntibodyHuman pancreas was stained with anti-Chromogranin A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01256-3-chga-primary-antibodies-ihc-testing-3.jpgAnti-Chromogranin A Rabbit Monoclonal AntibodyHuman stomach was stained with anti-Chromogranin A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01256-3-chga-primary-antibodies-ihc-testing-4.jpgAnti-Chromogranin A Rabbit Monoclonal AntibodyMouse pancreas was stained with anti-Chromogranin A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01256-3-chga-primary-antibodies-wb-testing-1.jpgAnti-Chromogranin A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Chromogranin A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Predicted band size: 51kDa Observed band size: 80kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-beta-actin-rabbit-monoclonal-antibody-m01263-7-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-7-actb-primary-antibodies-wb-testing-1.jpgAnti-β-actin Rabbit Monoclonal AntibodyWhole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-β-actin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1:Hela Predicted band size: 42kDa Observed band size: 42kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-beta-actin-rabbit-monoclonal-antibody-m01263-8-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-8-actb-primary-antibodies-ihc-testing-1.jpgAnti-β Actin Rabbit Monoclonal AntibodyHuman colon was stained with anti-β Actin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-8-actb-primary-antibodies-wb-testing-1.jpgAnti-β Actin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-β Actin antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: HeLa Lane 3: C6 Predicted band size: 42kDa Observed band size: 42kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glutaminase-c-rabbit-monoclonal-antibody-m01272-4-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01272-4-gls-primary-antibodies-ihc-testing-1.jpgAnti-Glutaminase C Rabbit Monoclonal AntibodyMouse colon was stained with Anti-Glutaminase C rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01272-4-gls-primary-antibodies-ihc-testing-2.jpgAnti-Glutaminase C Rabbit Monoclonal AntibodyHuman kidney was stained with Anti-Glutaminase C rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01272-4-gls-primary-antibodies-ihc-testing-3.jpgAnti-Glutaminase C Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with Anti-Glutaminase C rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01272-4-gls-primary-antibodies-wb-testing-1.jpgAnti-Glutaminase C Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Glutaminase C antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: K562 Lane 3: U-14 Lane 4: Rat womb Lane 5: Mouse spleen Predicted band size: 68kDa Observed band size: 68kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hsp60-rabbit-monoclonal-antibody-m01280-6-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01280-6-hspd1-primary-antibodies-ihc-testing-1.jpgAnti-Hsp60 Rabbit Monoclonal AntibodyMouse liver was stained with anti-Hsp60 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01280-6-hspd1-primary-antibodies-ihc-testing-2.jpgAnti-Hsp60 Rabbit Monoclonal AntibodyRat liver was stained with anti-Hsp60 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01280-6-hspd1-primary-antibodies-ihc-testing-3.jpgAnti-Hsp60 Rabbit Monoclonal AntibodyHuman liver was stained with anti-Hsp60 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01280-6-hspd1-primary-antibodies-wb-testing-1.jpgAnti-Hsp60 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Hsp60 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: T47D Lane 3: NIH-3T3 Lane 4: C6 Predicted band size: 60kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd43-rabbit-monoclonal-antibody-m01296-3-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01296-3-spn-primary-antibodies-ihc-testing-1.jpgAnti-CD43 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-CD43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01296-3-spn-primary-antibodies-ihc-testing-2.jpgAnti-CD43 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-CD43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01296-3-spn-primary-antibodies-ihc-testing-3.jpgAnti-CD43 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-CD43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01296-3-spn-primary-antibodies-ihc-testing-4.jpgAnti-CD43 Rabbit Monoclonal AntibodyRat spleen was stained with anti-CD43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01296-3-spn-primary-antibodies-wb-testing-1.jpgAnti-CD43 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD43 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HL-60 Predicted band size: 40kDa Observed band size: 130kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tcf7-rabbit-monoclonal-antibody-m01315-1-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01315-1-tcf7-primary-antibodies-ihc-testing-1.jpgAnti-TCF7 Rabbit Monoclonal AntibodyRat lung was stained with anti-TCF7 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01315-1-tcf7-primary-antibodies-ihc-testing-2.jpgAnti-TCF7 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-TCF7 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01315-1-tcf7-primary-antibodies-wb-testing-1.jpgAnti-TCF7 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TCF7 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HT-29 Lane 2: Rat spleen Predicted band size: 42kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-neuropilin-1-rabbit-monoclonal-antibody-m01324-4-boster.html2026-03-16T05:09:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01324-4-nrp1-primary-antibodies-ihc-testing-1.jpgAnti-Neuropilin 1 Rabbit Monoclonal AntibodyHuman liver was stained with Anti-Neuropilin 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01324-4-nrp1-primary-antibodies-ihc-testing-2.jpgAnti-Neuropilin 1 Rabbit Monoclonal AntibodyRat liver was stained with anti-Neuropilin 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01324-4-nrp1-primary-antibodies-ihc-testing-3.jpgAnti-Neuropilin 1 Rabbit Monoclonal AntibodyMouse liver was stained with anti-Neuropilin 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01324-4-nrp1-primary-antibodies-wb-testing-1.jpgAnti-Neuropilin 1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Neuropilin 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse lung Predicted band size: 103kDa Observed band size: 135kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cdc25c-rabbit-monoclonal-antibody-m01343-3-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01343-3-cdc25c-primary-antibodies-wb-testing-1.jpgAnti-Cdc25C Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cdc25C antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-14 Lane 2: HeLa Lane 3: K562 Predicted band size: 53kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytokeratin-18-rabbit-monoclonal-antibody-m01357-5-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01357-5-krt18-primary-antibodies-ihc-testing-1.jpgAnti-Cytokeratin 18 Rabbit Monoclonal AntibodyHuman colon was stained with anti-Cytokeratin 18 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01357-5-krt18-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 18 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Cytokeratin 18 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01357-5-krt18-primary-antibodies-ihc-testing-3.jpgAnti-Cytokeratin 18 Rabbit Monoclonal AntibodyMouse kidney was stained with anti-Cytokeratin 18 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01357-5-krt18-primary-antibodies-ihc-testing-4.jpgAnti-Cytokeratin 18 Rabbit Monoclonal AntibodyRat kidney was stained with anti-Cytokeratin 18 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01357-5-krt18-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 18 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 18 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: A431 Lane 3: HePa1-6 Lane 4: Rat liver Predicted band size: 48kDa Observed band size: 48kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cathepsin-d-rabbit-monoclonal-antibody-m01361-1-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01361-1-ctsd-primary-antibodies-ihc-testing-1.jpgAnti-Cathepsin D Rabbit Monoclonal AntibodyRat liver was stained with anti-Cathepsin D rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01361-1-ctsd-primary-antibodies-ihc-testing-2.jpgAnti-Cathepsin D Rabbit Monoclonal AntibodyMouse liver was stained with anti-Cathepsin D rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01361-1-ctsd-primary-antibodies-ihc-testing-3.jpgAnti-Cathepsin D Rabbit Monoclonal AntibodyHuman prostate was stained with anti-Cathepsin D rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01361-1-ctsd-primary-antibodies-ihc-testing-4.jpgAnti-Cathepsin D Rabbit Monoclonal AntibodyHuman liver was stained with anti-Cathepsin D rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01361-1-ctsd-primary-antibodies-wb-testing-5.jpgAnti-Cathepsin D Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cathepsin D antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: MCF7 Predicted band size: 44kDa Observed band size: 30kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01361-1-ctsd-primary-antibodies-wb-testing-6.jpgAnti-Cathepsin D Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cathepsin D antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: Mouse brain Predicted band size: 44kDa Observed band size: 44kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-periostin-rabbit-monoclonal-antibody-m01378-4-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01378-4-postn-primary-antibodies-ihc-testing-1.jpgAnti-Periostin Rabbit Monoclonal AntibodyHuman breast was stained with anti-Periostin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01378-4-postn-primary-antibodies-ihc-testing-2.jpgAnti-Periostin Rabbit Monoclonal AntibodyHuman colon was stained with anti-Periostin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01378-4-postn-primary-antibodies-wb-testing-1.jpgAnti-Periostin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Periostin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: T47D Predicted band size: 93kDa Observed band size: 93kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hexokinase-ii-rabbit-monoclonal-antibody-m01389-4-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01389-4-hk2-primary-antibodies-ihc-testing-1.jpgAnti-Hexokinase II Rabbit Monoclonal AntibodyRat kidney was stained with Anti-Hexokinase II rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01389-4-hk2-primary-antibodies-ihc-testing-2.jpgAnti-Hexokinase II Rabbit Monoclonal AntibodyMouse kidney was stained with Anti-Hexokinase II rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01389-4-hk2-primary-antibodies-ihc-testing-3.jpgAnti-Hexokinase II Rabbit Monoclonal AntibodyHuman skin was stained with Anti-Hexokinase II rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01389-4-hk2-primary-antibodies-wb-testing-1.jpgAnti-Hexokinase II Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Hexokinase II antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: LN18 Predicted band size: 87kDa Observed band size: 100kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aldh1a1-rabbit-monoclonal-antibody-m01392-3-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01392-3-aldh1a1-primary-antibodies-ihc-testing-1.jpgAnti-ALDH1A1 Rabbit Monoclonal AntibodyMouselung was stained with anti-ALDH1A1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01392-3-aldh1a1-primary-antibodies-ihc-testing-2.jpgAnti-ALDH1A1 Rabbit Monoclonal AntibodyRatlung was stained with anti-ALDH1A1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01392-3-aldh1a1-primary-antibodies-ihc-testing-3.jpgAnti-ALDH1A1 Rabbit Monoclonal AntibodyHumanlung was stained with anti-ALDH1A1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01392-3-aldh1a1-primary-antibodies-wb-testing-1.jpgAnti-ALDH1A1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ALDH1A1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: A549 Lane 3: Mouse colon Predicted band size: 55kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-iba1-rabbit-monoclonal-antibody-m01394-5-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-5-aif1-primary-antibodies-ihc-testing-1.jpgAnti-Iba1 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Iba1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-5-aif1-primary-antibodies-ihc-testing-2.jpgAnti-Iba1 Rabbit Monoclonal AntibodyMouse liver was stained with anti-Iba1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-5-aif1-primary-antibodies-ihc-testing-3.jpgAnti-Iba1 Rabbit Monoclonal AntibodyRat liver was stained with anti-Iba1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-5-aif1-primary-antibodies-ihc-testing-4.jpgAnti-Iba1 Rabbit Monoclonal AntibodyHuman liver was stained with anti-Iba1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-5-aif1-primary-antibodies-wb-testing-5.jpgAnti-Iba1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Iba1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat brain Lane 2: RAW264.7 Lane 3: THP-1 Predicted band size: 16kDa Observed band size: 16kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01394-5-aif1-primary-antibodies-wb-testing-6.jpgAnti-Iba1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Iba1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse spleen Lane 2: THP-1 Lane 3: Rat testis Predicted band size: 16kDa Observed band size: 16kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-collagen-iv-rabbit-monoclonal-antibody-m01411-2-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01411-2-col4a1-primary-antibodies-ihc-testing-1.jpgAnti-Collagen IV Rabbit Monoclonal AntibodyHuman appendix was stained with Anti-Collagen IV rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01411-2-col4a1-primary-antibodies-wb-testing-1.jpgAnti-Collagen IV Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Collagen IV antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: A431 Predicted band size: 160kDa Observed band size: 200kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytokeratin-8-rabbit-monoclonal-antibody-m01421-6-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01421-6-krt8-primary-antibodies-ihc-testing-1.jpgAnti-Cytokeratin 8 Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-Cytokeratin 8 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01421-6-krt8-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 8 Rabbit Monoclonal AntibodyMouse stomach was stained with anti-Cytokeratin 8 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01421-6-krt8-primary-antibodies-ihc-testing-3.jpgAnti-Cytokeratin 8 Rabbit Monoclonal AntibodyRat stomach was stained with anti-Cytokeratin 8 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01421-6-krt8-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 8 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 8 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: A431 Lane 3: Mouse liver Predicted band size: 54kDa Observed band size: 54kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smad5-rabbit-monoclonal-antibody-m01423-2-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01423-2-smad5-primary-antibodies-ihc-testing-1.jpgAnti-Smad5 Rabbit Monoclonal AntibodyHuman liver was stained with anti-Smad5 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01423-2-smad5-primary-antibodies-wb-testing-1.jpgAnti-Smad5 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Smad5 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C2C12 Lane 2: C6 Predicted band size: 55kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytokeratin-14-rabbit-monoclonal-antibody-m01432-4-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01432-4-krt14-primary-antibodies-ihc-testing-1.jpgAnti-Cytokeratin 14 Rabbit Monoclonal AntibodyMouse skin was stained with anti-Cytokeratin 14 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01432-4-krt14-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 14 Rabbit Monoclonal AntibodyHuman cervical squamous cell carcinoma was stained with anti-Cytokeratin 14 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01432-4-krt14-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 14 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 14 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: HaCat Lane 3: Rat skin Predicted band size: 52kDa Observed band size: 52kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tak1-rabbit-monoclonal-antibody-m01458-3-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01458-3-map3k7-primary-antibodies-wb-testing-1.jpgAnti-TAK1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TAK1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: HeLa Lane 3: U-14 Lane 4: Rat ovary Predicted band size: 67kDa Observed band size: 67kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-raptor-rabbit-monoclonal-antibody-m01463-2-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01463-2-rptor-primary-antibodies-ihc-testing-1.jpgAnti-Raptor Rabbit Monoclonal AntibodyHuman colon was stained with anti-Raptor rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01463-2-rptor-primary-antibodies-wb-testing-1.jpgAnti-Raptor Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Raptor antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HeLa Lane 3: C2C12 Predicted band size: 149kDa Observed band size: 149kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-s6k1-rabbit-monoclonal-antibody-m01475-6-boster.html2026-03-16T05:09:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01475-6-rps6kb1-primary-antibodies-ihc-testing-1.jpgAnti-S6K1 Rabbit Monoclonal AntibodyMouse brain was stained with anti-S6K1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01475-6-rps6kb1-primary-antibodies-ihc-testing-2.jpgAnti-S6K1 Rabbit Monoclonal AntibodyRat brain was stained with anti-S6K1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01475-6-rps6kb1-primary-antibodies-wb-testing-1.jpgAnti-S6K1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-S6K1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Rat brain Lane 3: Neuro-2a Predicted band size: 75kDa Observed band size: 70kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ctip2-rabbit-monoclonal-antibody-m01485-2-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01485-2-bcl11b-primary-antibodies-ihc-testing-1.jpgAnti-Ctip2 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-Ctip2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01485-2-bcl11b-primary-antibodies-ihc-testing-2.jpgAnti-Ctip2 Rabbit Monoclonal AntibodyRat spleen was stained with anti-Ctip2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01485-2-bcl11b-primary-antibodies-ihc-testing-3.jpgAnti-Ctip2 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Ctip2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01485-2-bcl11b-primary-antibodies-wb-testing-1.jpgAnti-Ctip2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Ctip2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Predicted band size: 96kDa Observed band size: 96kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-met-rabbit-monoclonal-antibody-m01488-10-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01488-10-met-primary-antibodies-ihc-testing-1.jpgAnti-Met Rabbit Monoclonal AntibodyRat colon was stained with anti-Met rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01488-10-met-primary-antibodies-ihc-testing-2.jpgAnti-Met Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-Met rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01488-10-met-primary-antibodies-wb-testing-1.jpgAnti-Met Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Met antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Lane 2: 3T3-L1 Lane 3: A549 Lane 4: HeLa Predicted band size: 156kDa Observed band size: 170kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd31-rabbit-monoclonal-antibody-m01513-6-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01513-6-pecam1-primary-antibodies-ihc-testing-1.jpgAnti-CD31 Rabbit Monoclonal AntibodyHuman placenta was stained with Anti-CD31 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01513-6-pecam1-primary-antibodies-ihc-testing-2.jpgAnti-CD31 Rabbit Monoclonal AntibodyHuman tonsil was stained with Anti-CD31 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01513-6-pecam1-primary-antibodies-wb-testing-1.jpgAnti-CD31 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD31 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: THP-1 Predicted band size: 83kDa Observed band size: 130kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd31-rabbit-monoclonal-antibody-m01513-7-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01513-7-pecam1-primary-antibodies-ihc-testing-1.jpgAnti-CD31 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-CD31 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01513-7-pecam1-primary-antibodies-ihc-testing-2.jpgAnti-CD31 Rabbit Monoclonal AntibodyMouse lung was stained with anti-CD31 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01513-7-pecam1-primary-antibodies-ihc-testing-3.jpgAnti-CD31 Rabbit Monoclonal AntibodyRat lung was stained with anti-CD31 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01513-7-pecam1-primary-antibodies-wb-testing-1.jpgAnti-CD31 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD31 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: THP-1 Lane 2: Rat spleen Predicted band size: 81kDa Observed band size: 130kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lc3b-rabbit-monoclonal-antibody-m01524-2-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01524-2-map1lc3b-primary-antibodies-ihc-testing-1.jpgAnti-LC3B Rabbit Monoclonal AntibodyMouse brain was stained with anti-LC3B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01524-2-map1lc3b-primary-antibodies-ihc-testing-2.jpgAnti-LC3B Rabbit Monoclonal AntibodyRat brain was stained with anti-LC3B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01524-2-map1lc3b-primary-antibodies-ihc-testing-3.jpgAnti-LC3B Rabbit Monoclonal AntibodyHuman brain was stained with anti-LC3B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01524-2-map1lc3b-primary-antibodies-wb-testing-1.jpgAnti-LC3B Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LC3B antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: Rat spleen Lane 3: Mouse brain Lane 3: U-87 MG Predicted band size: 14,16kDa Observed band size: 14,16kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fabp4-rabbit-monoclonal-antibody-m01528-3-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01528-3-fabp4-primary-antibodies-ihc-testing-1.jpgAnti-FABP4 Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with Anti-FABP4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01528-3-fabp4-primary-antibodies-ihc-testing-2.jpgAnti-FABP4 Rabbit Monoclonal AntibodyMouse pancreas was stained with Anti-FABP4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01528-3-fabp4-primary-antibodies-ihc-testing-3.jpgAnti-FABP4 Rabbit Monoclonal AntibodyRat pancreas was stained with Anti-FABP4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01528-3-fabp4-primary-antibodies-ihc-testing-4.jpgAnti-FABP4 Rabbit Monoclonal AntibodyRat pancreas was stained with anti-FABP4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01528-3-fabp4-primary-antibodies-wb-testing-1.jpgAnti-FABP4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FABP4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat heart Lane 2: RT4 Predicted band size: 15kDa Observed band size: 15kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lc3a-rabbit-monoclonal-antibody-m01543-4-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01543-4-map1lc3a-primary-antibodies-ihc-testing-1.jpgAnti-LC3A Rabbit Monoclonal AntibodyMouse prostate was stained with anti-LC3A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01543-4-map1lc3a-primary-antibodies-ihc-testing-2.jpgAnti-LC3A Rabbit Monoclonal AntibodyRat colon was stained with anti-LC3A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01543-4-map1lc3a-primary-antibodies-ihc-testing-3.jpgAnti-LC3A Rabbit Monoclonal AntibodyHuman prostate was stained with anti-LC3A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01543-4-map1lc3a-primary-antibodies-wb-testing-1.jpgAnti-LC3A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LC3A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse brain Lane 2: SH-SY5Y Lane 3: A172 Lane 4: C6 Predicted band size: 14kDa Observed band size: 16kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bag3-rabbit-monoclonal-antibody-m01547-2-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01547-2-bag3-primary-antibodies-ihc-testing-1.jpgAnti-Bag3 Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-Bag3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01547-2-bag3-primary-antibodies-ihc-testing-2.jpgAnti-Bag3 Rabbit Monoclonal AntibodyMouse lung was stained with anti-Bag3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01547-2-bag3-primary-antibodies-ihc-testing-3.jpgAnti-Bag3 Rabbit Monoclonal AntibodyRat lung was stained with anti-Bag3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01547-2-bag3-primary-antibodies-wb-testing-1.jpgAnti-Bag3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Bag3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HeLa Lane 3: C6 Lane 4: 3T3-L1 Predicted band size: 62kDa Observed band size: 80kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bim-rabbit-monoclonal-antibody-m01552-1-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01552-1-bcl2l11-primary-antibodies-wb-testing-1.jpgAnti-Bim Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Bim antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: Raji Lane 3: Rat spleen Lane 4: MOLT-4 Predicted band size: 22kDa Observed band size: 22kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mmp7-rabbit-monoclonal-antibody-m01553-2-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01553-2-mmp7-primary-antibodies-ihc-testing-1.jpgAnti-MMP7 Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-MMP7 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01553-2-mmp7-primary-antibodies-ihc-testing-2.jpgAnti-MMP7 Rabbit Monoclonal AntibodyHuman gastric carcinoma was stained with anti-MMP7 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01553-2-mmp7-primary-antibodies-wb-testing-1.jpgAnti-MMP7 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MMP7 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: HT-29 Predicted band size: 30kDa Observed band size: 30kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rps6-rabbit-monoclonal-antibody-m01567-1-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01567-1-rps6-primary-antibodies-ihc-testing-1.jpgAnti-RPS6 Rabbit Monoclonal AntibodyRat brain was stained with anti-RPS6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01567-1-rps6-primary-antibodies-ihc-testing-2.jpgAnti-RPS6 Rabbit Monoclonal AntibodyHuman brain was stained with anti-RPS6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01567-1-rps6-primary-antibodies-ihc-testing-3.jpgAnti-RPS6 Rabbit Monoclonal AntibodyMouse brain was stained with anti-RPS6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01567-1-rps6-primary-antibodies-wb-testing-1.jpgAnti-RPS6 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RPS6 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: C6 Lane 3: HeLa Lane 4: K562 Predicted band size: 29kDa Observed band size: 31kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aif-m1-rabbit-monoclonal-antibody-m01571-4-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-4-aifm1-primary-antibodies-ihc-testing-1.jpgAnti-AIF-M1 Rabbit Monoclonal AntibodyMousekidney was stained with anti-AIF-M1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-4-aifm1-primary-antibodies-ihc-testing-2.jpgAnti-AIF-M1 Rabbit Monoclonal AntibodyRatkidney was stained with anti-AIF-M1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-4-aifm1-primary-antibodies-ihc-testing-3.jpgAnti-AIF-M1 Rabbit Monoclonal AntibodyHumankidney was stained with anti-AIF-M1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01571-4-aifm1-primary-antibodies-wb-testing-1.jpgAnti-AIF-M1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-AIF-M1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: C6 Lane 3: NIH-3T3 Lane 4: HEK293 Predicted band size: 67kDa Observed band size: 67kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lamp2-rabbit-monoclonal-antibody-m01573-3-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01573-3-lamp2-primary-antibodies-ihc-testing-1.jpgAnti-LAMP2 Rabbit Monoclonal AntibodyMouse liver was stained with anti-LAMP2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01573-3-lamp2-primary-antibodies-ihc-testing-2.jpgAnti-LAMP2 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-LAMP2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01573-3-lamp2-primary-antibodies-ihc-testing-3.jpgAnti-LAMP2 Rabbit Monoclonal AntibodyHuman liver was stained with anti-LAMP2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01573-3-lamp2-primary-antibodies-wb-testing-1.jpgAnti-LAMP2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LAMP2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Predicted band size: 45kDa Observed band size: 120kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-n-cadherin-rabbit-monoclonal-antibody-m01577-6-boster.html2026-03-16T05:09:20+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-n-cadherin-rabbit-monoclonal-antibody-m01577-7-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01577-7-cdh2-primary-antibodies-ihc-testing-1.jpgAnti-N Cadherin Rabbit Monoclonal AntibodyHuman kidney carcinoma was stained with anti-N Cadherin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01577-7-cdh2-primary-antibodies-ihc-testing-2.jpgAnti-N Cadherin Rabbit Monoclonal AntibodyHuman liver was stained with anti-N Cadherin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01577-7-cdh2-primary-antibodies-ihc-testing-3.jpgAnti-N Cadherin Rabbit Monoclonal AntibodyMouse liver was stained with anti-N Cadherin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01577-7-cdh2-primary-antibodies-ihc-testing-4.jpgAnti-N Cadherin Rabbit Monoclonal AntibodyRat liver was stained with anti-N Cadherin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01577-7-cdh2-primary-antibodies-wb-testing-1.jpgAnti-N Cadherin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-N Cadherin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse brain Predicted band size: 125kDa Observed band size: 130kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-claudin-1-rabbit-monoclonal-antibody-m01585-2-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01585-2-cldn1-primary-antibodies-ihc-testing-1.jpgAnti-Claudin 1 Rabbit Monoclonal AntibodyRat stomach tissue was stained with anti-Claudin 1 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01585-2-cldn1-primary-antibodies-ihc-testing-2.jpgAnti-Claudin 1 Rabbit Monoclonal AntibodyRat esophagus tissue was stained with anti-Claudin 1 rabbit Antibody https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-claudin-1-rabbit-monoclonal-antibody-m01585-3-boster.html2026-03-16T05:09:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01585-3-cldn1-primary-antibodies-ihc-testing-1.jpgAnti-Claudin 1 Rabbit Monoclonal AntibodyMouse stomach was stained with anti-Claudin 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01585-3-cldn1-primary-antibodies-ihc-testing-2.jpgAnti-Claudin 1 Rabbit Monoclonal AntibodyRat stomach was stained with anti-Claudin 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01585-3-cldn1-primary-antibodies-ihc-testing-3.jpgAnti-Claudin 1 Rabbit Monoclonal AntibodyHuman breast was stained with anti-Claudin 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01585-3-cldn1-primary-antibodies-wb-testing-1.jpgAnti-Claudin 1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Claudin 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: A431 Lane 3: Mouse skin Predicted band size: 22kDa Observed band size: 19kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-enos-rabbit-monoclonal-antibody-m01604-2-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01604-2-nos3-primary-antibodies-ihc-testing-1.jpgAnti-eNOS Rabbit Monoclonal AntibodyRat spleen was stained with anti-eNOS rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01604-2-nos3-primary-antibodies-ihc-testing-2.jpgAnti-eNOS Rabbit Monoclonal AntibodyMouse liver was stained with anti-eNOS rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01604-2-nos3-primary-antibodies-ihc-testing-3.jpgAnti-eNOS Rabbit Monoclonal AntibodyHuman placenta was stained with anti-eNOS rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01604-2-nos3-primary-antibodies-ihc-testing-4.jpgAnti-eNOS Rabbit Monoclonal AntibodyHuman spleen was stained with anti-eNOS rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01604-2-nos3-primary-antibodies-wb-testing-1.jpgAnti-eNOS Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-eNOS antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Predicted band size: 133kDa Observed band size: 133kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slug-rabbit-monoclonal-antibody-m01615-4-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01615-4-snai2-primary-antibodies-wb-testing-1.jpgAnti-Slug Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Slug antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MES-SA Lane 2: A431 Predicted band size: 30kDa Observed band size: 36kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd21-rabbit-monoclonal-antibody-m01632-2-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01632-2-cr2-primary-antibodies-ihc-testing-1.jpgAnti-CD21 Rabbit Monoclonal AntibodyRat spleen was stained with anti-CD21 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01632-2-cr2-primary-antibodies-ihc-testing-2.jpgAnti-CD21 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-CD21 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01632-2-cr2-primary-antibodies-ihc-testing-3.jpgAnti-CD21 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-CD21 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01632-2-cr2-primary-antibodies-ihc-testing-4.jpgAnti-CD21 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-CD21 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01632-2-cr2-primary-antibodies-wb-testing-1.jpgAnti-CD21 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD21 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Raji Lane 2: Daudi Lane 3: Mouse spleen Predicted band size: 113kDa Observed band size: 155kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd146-rabbit-monoclonal-antibody-m01683-7-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-7-mcam-primary-antibodies-ihc-testing-1.jpgAnti-CD146 Rabbit Monoclonal AntibodyHuman colon was stained with anti-CD146 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-7-mcam-primary-antibodies-ihc-testing-2.jpgAnti-CD146 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-CD146 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-7-mcam-primary-antibodies-ihc-testing-3.jpgAnti-CD146 Rabbit Monoclonal AntibodyMouse colon was stained with anti-CD146 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-7-mcam-primary-antibodies-ihc-testing-4.jpgAnti-CD146 Rabbit Monoclonal AntibodyRat colon was stained with anti-CD146 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01683-7-mcam-primary-antibodies-wb-testing-1.jpgAnti-CD146 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD146 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: HepG2 Predicted band size: 72kDa Observed band size: 125kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hsp90-rabbit-monoclonal-antibody-m01692-5-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01692-5-hsp90ab1-primary-antibodies-ihc-testing-1.jpgAnti-Hsp90 Rabbit Monoclonal AntibodyHuman tonsil testis was stained with Anti-HSP90 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01692-5-hsp90ab1-primary-antibodies-ihc-testing-2.jpgAnti-Hsp90 Rabbit Monoclonal AntibodyMouse testis was stained with Anti-HSP90 rabbit Antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01692-5-hsp90ab1-primary-antibodies-wb-testing-1.jpgAnti-Hsp90 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Hsp90 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: PC12 Lane 2: PANC-1 Lane 3: HepG2 Predicted band size: 85kDa Observed band size: 85kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alpp-rabbit-monoclonal-antibody-m01718-6-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01718-6-alpp-primary-antibodies-ihc-testing-1.jpgAnti-ALPP Rabbit Monoclonal AntibodyHuman placenta was stained with anti-ALPP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01718-6-alpp-primary-antibodies-wb-testing-1.jpgAnti-ALPP Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ALPP antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: JAR Lane 3: Rat small intestine Predicted band size: 58kDa Observed band size: 70kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cardiac-troponin-i-rabbit-monoclonal-antibody-m01720-3-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01720-3-tnni3-primary-antibodies-ihc-testing-1.jpgAnti-Cardiac Troponin I Rabbit Monoclonal AntibodyMouse cardiac muscle was stained with anti-Cardiac Troponin I rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01720-3-tnni3-primary-antibodies-ihc-testing-2.jpgAnti-Cardiac Troponin I Rabbit Monoclonal AntibodyRat cardiac muscle was stained with anti-Cardiac Troponin I rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01720-3-tnni3-primary-antibodies-wb-testing-1.jpgAnti-Cardiac Troponin I Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cardiac Troponin I antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat heart Lane 2: Mouse heart Predicted band size: 24kDa Observed band size: 24kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gclc-rabbit-monoclonal-antibody-m01722-2-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01722-2-gclc-primary-antibodies-wb-testing-1.jpgAnti-GCLC Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GCLC antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: Mouse brain Lane 4: Raji Predicted band size: 73kDa Observed band size: 73kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd99-rabbit-monoclonal-antibody-m01724-3-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01724-3-cd99-primary-antibodies-ihc-testing-1.jpgAnti-CD99 Rabbit Monoclonal AntibodyHuman prostate carcinoma was stained with anti-CD99 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01724-3-cd99-primary-antibodies-wb-testing-1.jpgAnti-CD99 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD99 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Predicted band size: 19kDa Observed band size: 30kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mek4-rabbit-monoclonal-antibody-m01725-3-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01725-3-map2k4-primary-antibodies-wb-testing-1.jpgAnti-MEK4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MEK4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A431 Lane 2: HeLa Lane 3: NIH-3T3 Lane 4: Rat brain Predicted band size: 44kDa Observed band size: 44kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mypt1-rabbit-monoclonal-antibody-m01743-1-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01743-1-ppp1r12a-primary-antibodies-ihc-testing-1.jpgAnti-MYPT1 Rabbit Monoclonal AntibodyHuman placenta was stained with anti-MYPT1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01743-1-ppp1r12a-primary-antibodies-wb-testing-1.jpgAnti-MYPT1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MYPT1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Daudi Lane 2: Saos-2 Predicted band size: 140kDa Observed band size: 140kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01743-1-ppp1r12a-primary-antibodies-wb-testing-2.jpgAnti-MYPT1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-MYPT1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Predicted band size: 140kDa Observed band size: 140kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ezrin-rabbit-monoclonal-antibody-m01750-2-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01750-2-ezr-primary-antibodies-ihc-testing-1.jpgAnti-Ezrin Rabbit Monoclonal AntibodyHuman bladder carcinoma was stained with anti-Ezrin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01750-2-ezr-primary-antibodies-ihc-testing-2.jpgAnti-Ezrin Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Ezrin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01750-2-ezr-primary-antibodies-ihc-testing-3.jpgAnti-Ezrin Rabbit Monoclonal AntibodyMouse kidney was stained with anti-Ezrin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01750-2-ezr-primary-antibodies-ihc-testing-4.jpgAnti-Ezrin Rabbit Monoclonal AntibodyRat kidney was stained with anti-Ezrin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01750-2-ezr-primary-antibodies-wb-testing-1.jpgAnti-Ezrin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Ezrin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HCT-116 Lane 2: HeLa Lane 3: C6 Lane 4: NIH-3T3 Predicted band size: 69kDa Observed band size: 80kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alix-rabbit-monoclonal-antibody-m01751-4-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-4-pdcd6ip-primary-antibodies-ihc-testing-1.jpgAnti-ALIX Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with Anti-ALIX rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01751-4-pdcd6ip-primary-antibodies-wb-testing-1.jpgAnti-ALIX Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ALIX antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: HepG2 Lane 3: Jurkat Lane 4: HEK293 Lane 5: Mouse liver Predicted band size: 96kDa Observed band size: 96kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sdha-rabbit-monoclonal-antibody-m01753-2-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01753-2-sdha-primary-antibodies-ihc-testing-1.jpgAnti-SDHA Rabbit Monoclonal AntibodyHuman kidney was stained with Anti-SDHA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01753-2-sdha-primary-antibodies-ihc-testing-2.jpgAnti-SDHA Rabbit Monoclonal AntibodyMouse kidney was stained with Anti-SDHA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01753-2-sdha-primary-antibodies-ihc-testing-3.jpgAnti-SDHA Rabbit Monoclonal AntibodyRat kidney was stained with Anti-SDHA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01753-2-sdha-primary-antibodies-wb-testing-1.jpgAnti-SDHA Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SDHA antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Mouse brain Lane 3: Rat brain Predicted band size: 73kDa Observed band size: 73kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mettl3-rabbit-monoclonal-antibody-m01758-2-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01758-2-mettl3-primary-antibodies-ihc-testing-1.jpgAnti-METTL3 Rabbit Monoclonal AntibodyHuman prostate cancer was stained with Anti-METTL3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01758-2-mettl3-primary-antibodies-ihc-testing-2.jpgAnti-METTL3 Rabbit Monoclonal AntibodyMouse testis was stained with Anti-METTL3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01758-2-mettl3-primary-antibodies-wb-testing-1.jpgAnti-METTL3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-METTL3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NCCIT Lane 2: C6 Lane 3: Neuro-2a Lane 4: Raji Predicted band size: 64kDa Observed band size: 70kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rrm1-rabbit-monoclonal-antibody-m01764-3-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01764-3-rrm1-primary-antibodies-wb-testing-1.jpgAnti-RRM1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RRM1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: U2OS Lane 2: 4T1 Lane 3: L6 Predicted band size: 90kDa Observed band size: 90kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd133-rabbit-monoclonal-antibody-m01767-7-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01767-7-prom1-primary-antibodies-ihc-testing-1.jpgAnti-CD133 Rabbit Monoclonal AntibodyHuman bladder carcinoma was stained with anti-CD133 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01767-7-prom1-primary-antibodies-wb-testing-1.jpgAnti-CD133 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD133 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Caco-2 Lane 2: HT-29 Predicted band size: 97kDa Observed band size: 133kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atg3-rabbit-monoclonal-antibody-m01768-3-boster.html2026-03-16T05:09:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01768-3-atg3-primary-antibodies-wb-testing-1.jpgAnti-ATG3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ATG3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: HeLa Lane 3: C6 Predicted band size: 36kDa Observed band size: 36kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atgl-rabbit-monoclonal-antibody-m01800-1-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01800-1-pnpla2-primary-antibodies-ihc-testing-1.jpgAnti-ATGL Rabbit Monoclonal AntibodyRat white adipose was stained with anti-ATGL rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01800-1-pnpla2-primary-antibodies-wb-testing-1.jpgAnti-ATGL Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti- ATGL antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: K562 Lane 3: C6 Predicted band size: 55kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sall4-rabbit-monoclonal-antibody-m01827-1-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01827-1-sall4-primary-antibodies-wb-testing-1.jpgAnti-Sall4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Sall4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: JAR Lane 2: NCCIT Predicted band size: 112kDa Observed band size: 165kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-beta-galactosidase-rabbit-monoclonal-antibody-m01829-4-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01829-4-glb1-primary-antibodies-ihc-testing-1.jpgAnti-β-Galactosidase Rabbit Monoclonal AntibodyMouse liver was stained with anti-β-Galactosidase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01829-4-glb1-primary-antibodies-ihc-testing-2.jpgAnti-β-Galactosidase Rabbit Monoclonal AntibodyRat liver was stained with anti-β-Galactosidase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01829-4-glb1-primary-antibodies-wb-testing-1.jpgAnti-β-Galactosidase Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-β-Galactosidase antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse brain Lane 2: C6 Lane 3: HeLa Lane 4: K562 Predicted band size: 76kDa Observed band size: 100kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-peroxiredoxin-1-rabbit-monoclonal-antibody-m01845-3-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01845-3-prdx1-primary-antibodies-ihc-testing-1.jpgAnti-Peroxiredoxin 1 Rabbit Monoclonal AntibodyRat kidney was stained with anti-Peroxiredoxin 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01845-3-prdx1-primary-antibodies-ihc-testing-2.jpgAnti-Peroxiredoxin 1 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Peroxiredoxin 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01845-3-prdx1-primary-antibodies-ihc-testing-3.jpgAnti-Peroxiredoxin 1 Rabbit Monoclonal AntibodyHuman thyroid was stained with anti-Peroxiredoxin 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01845-3-prdx1-primary-antibodies-wb-testing-1.jpgAnti-Peroxiredoxin 1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Peroxiredoxin 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HepG2 Lane 3: Mouse liver Lane 4: Rat liver Predicted band size: 22kDa Observed band size: 26kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tubulin-beta3-rabbit-monoclonal-antibody-m01857-5-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01857-5-tubb3-primary-antibodies-wb-testing-1.jpgAnti-Tubulin β3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Tubulin β3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1:A431 Lane 2:HEK293 Predicted band size: 50kDa Observed band size: 50kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-beta3-tubulin-rabbit-monoclonal-antibody-m01857-6-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01857-6-tubb3-primary-antibodies-ihc-testing-1.jpgAnti-β3 Tubulin Rabbit Monoclonal AntibodyHuman appendix was stained with anti-β3 Tubulin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01857-6-tubb3-primary-antibodies-ihc-testing-2.jpgAnti-β3 Tubulin Rabbit Monoclonal AntibodyMouse brain was stained with anti-β3 Tubulin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01857-6-tubb3-primary-antibodies-wb-testing-1.jpgAnti-β3 Tubulin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-β3 Tubulin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: Mouse brain Lane 3: Rat brain Predicted band size: 50kDa Observed band size: 50kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rad21-rabbit-monoclonal-antibody-m01864-3-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01864-3-rad21-primary-antibodies-ihc-testing-1.jpgAnti-Rad21 Rabbit Monoclonal AntibodyRat stomach was stained with Anti-Rad21 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01864-3-rad21-primary-antibodies-ihc-testing-2.jpgAnti-Rad21 Rabbit Monoclonal AntibodyMouse stomach was stained with Anti-Rad21 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01864-3-rad21-primary-antibodies-ihc-testing-3.jpgAnti-Rad21 Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with Anti-Rad21 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01864-3-rad21-primary-antibodies-wb-testing-1.jpgAnti-Rad21 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Rad21 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Jurkat Lane 3: RAW264.7 Predicted band size: 72kDa Observed band size: 130kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gap43-rabbit-monoclonal-antibody-m01868-5-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01868-5-gap43-primary-antibodies-ihc-testing-1.jpgAnti-GAP43 Rabbit Monoclonal AntibodyHuman glioma was stained with anti-GAP43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01868-5-gap43-primary-antibodies-ihc-testing-2.jpgAnti-GAP43 Rabbit Monoclonal AntibodyMouse brain was stained with anti-GAP43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01868-5-gap43-primary-antibodies-ihc-testing-3.jpgAnti-GAP43 Rabbit Monoclonal AntibodyRat brain was stained with anti-GAP43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01868-5-gap43-primary-antibodies-ihc-testing-4.jpgAnti-GAP43 Rabbit Monoclonal AntibodyHuman brain was stained with anti-GAP43 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01868-5-gap43-primary-antibodies-wb-testing-1.jpgAnti-GAP43 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GAP43 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-87 MG Lane 2: Rat brain Lane 3: Neuro-2a Predicted band size: 25kDa Observed band size: 48kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tmem173-rabbit-monoclonal-antibody-m01871-3-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01871-3-tmem173-primary-antibodies-ihc-testing-1.jpgAnti-TMEM173 Rabbit Monoclonal AntibodyRat colon was stained with Anti-TMEM173 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01871-3-tmem173-primary-antibodies-ihc-testing-2.jpgAnti-TMEM173 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-TMEM173 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01871-3-tmem173-primary-antibodies-ihc-testing-3.jpgAnti-TMEM173 Rabbit Monoclonal AntibodyRat spleen was stained with anti-TMEM173 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01871-3-tmem173-primary-antibodies-ihc-testing-4.jpgAnti-TMEM173 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-TMEM173 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01871-3-tmem173-primary-antibodies-wb-testing-1.jpgAnti-TMEM173 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TMEM173 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: K562 Lane 3: Rat spleen Predicted band size: 42kDa Observed band size: 37kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rab-5a-rabbit-monoclonal-antibody-m01891-2-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01891-2-rab5a-primary-antibodies-ihc-testing-1.jpgAnti-Rab 5A Rabbit Monoclonal AntibodyRat kidney was stained with anti-Rab 5A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01891-2-rab5a-primary-antibodies-ihc-testing-2.jpgAnti-Rab 5A Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Rab 5A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01891-2-rab5a-primary-antibodies-ihc-testing-3.jpgAnti-Rab 5A Rabbit Monoclonal AntibodyMouse kidney was stained with anti-Rab 5A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01891-2-rab5a-primary-antibodies-wb-testing-1.jpgAnti-Rab 5A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Rab 5A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: Jurkat Lane 3: C6 Lane 4: Neuro-2a Predicted band size: 24kDa Observed band size: 24kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pp2a-alpha-beta-rabbit-monoclonal-antibody-m01893-2-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01893-2-ppp2ca-primary-antibodies-ihc-testing-1.jpgAnti-PP2A α/β Rabbit Monoclonal AntibodyHuman kidney was stained with anti-PP2A α/β rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01893-2-ppp2ca-primary-antibodies-ihc-testing-2.jpgAnti-PP2A α/β Rabbit Monoclonal AntibodyRat kidney was stained with anti-PP2A α/β rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01893-2-ppp2ca-primary-antibodies-wb-testing-1.jpgAnti-PP2A α/β Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti- PP2A α/β antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: Mouse brain Lane 4: SH-SY5Y Predicted band size: 36kDa Observed band size: 36kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pyruvate-dehydrogenase-e1-alpha-rabbit-monoclonal-antibody-m01906-2-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01906-2-pdha1-primary-antibodies-ihc-testing-1.jpgAnti-Pyruvate Dehydrogenase E1 α Rabbit Monoclonal AntibodyRat kidney was stained with anti-Pyruvate Dehydrogenase E1 α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01906-2-pdha1-primary-antibodies-ihc-testing-2.jpgAnti-Pyruvate Dehydrogenase E1 α Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Pyruvate Dehydrogenase E1 α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01906-2-pdha1-primary-antibodies-ihc-testing-3.jpgAnti-Pyruvate Dehydrogenase E1 α Rabbit Monoclonal AntibodyMouse kidney was stained with anti-Pyruvate Dehydrogenase E1 α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01906-2-pdha1-primary-antibodies-wb-testing-1.jpgAnti-Pyruvate Dehydrogenase E1 α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Pyruvate Dehydrogenase E1 α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: NIH-3T3 Lane 3: HeLa Lane 4: Rat kidney Predicted band size: 43kDa Observed band size: 43kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ikk-alpha-beta-rabbit-monoclonal-antibody-m01918-3-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01918-3-chuk-primary-antibodies-ihc-testing-1.jpgAnti-IKK α/β Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-IKK α/β rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01918-3-chuk-primary-antibodies-wb-testing-1.jpgAnti-IKK α/β Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IKK α/β antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: HeLa Lane 3: Rat womb Predicted band size: 86kDa Observed band size: 86kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ikk-alpha-rabbit-monoclonal-antibody-m01918-4-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01918-4-chuk-primary-antibodies-ihc-testing-1.jpgAnti-IKK α Rabbit Monoclonal AntibodyRat kidney was stained with anti-IKK α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01918-4-chuk-primary-antibodies-ihc-testing-2.jpgAnti-IKK α Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-IKK α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01918-4-chuk-primary-antibodies-ihc-testing-3.jpgAnti-IKK α Rabbit Monoclonal AntibodyMouse kidney was stained with anti-IKK α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01918-4-chuk-primary-antibodies-wb-testing-1.jpgAnti-IKK α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IKK α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: K562 Lane 3: 3T3-L1 Predicted band size: 85kDa Observed band size: 85kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-perk-rabbit-monoclonal-antibody-m01992-1-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01992-1-eif2ak3-primary-antibodies-wb-testing-1.jpgAnti-PERK Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-PERK antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: C6 Predicted band size: 125kDa Observed band size: 140kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tbc1d4-rabbit-monoclonal-antibody-m02004-1-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02004-1-tbc1d4-primary-antibodies-wb-testing-1.jpgAnti-TBC1D4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TBC1D4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: HEK293 Lane 3: RAW264.7 Predicted band size: 147kDa Observed band size: 160kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-melana-rabbit-monoclonal-antibody-m02033-4-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02033-4-mlana-primary-antibodies-ihc-testing-1.jpgAnti-MelanA Rabbit Monoclonal AntibodyHuman melanoma was stained with anti-MelanA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02033-4-mlana-primary-antibodies-wb-testing-1.jpgAnti-MelanA Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MelanA antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: B16F10 Lane 2: SK-MEL-28 Lane 3: SK-MES-1 Predicted band size: 13kDa Observed band size: 18kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c-jun-rabbit-monoclonal-antibody-m02038-3-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02038-3-jun-primary-antibodies-ihc-testing-1.jpgAnti-c-Jun Rabbit Monoclonal AntibodyHuman cervical carcinoma was stained with anti-c-Jun rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02038-3-jun-primary-antibodies-ihc-testing-2.jpgAnti-c-Jun Rabbit Monoclonal AntibodyMouse brain was stained with anti-c-Jun rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02038-3-jun-primary-antibodies-ihc-testing-3.jpgAnti-c-Jun Rabbit Monoclonal AntibodyRat brain was stained with anti-c-Jun rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02038-3-jun-primary-antibodies-wb-testing-1.jpgAnti-c-Jun Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti- c-Jun antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-12 Lane 2: HeLa Lane 3: HEK293 Lane 4: NIH-3T3 Predicted band size: 36kDa Observed band size: 43kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gpx4-rabbit-monoclonal-antibody-m02059-2-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02059-2-gpx4-primary-antibodies-wb-testing-1.jpgAnti-GPX4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Glutathione Peroxidase 4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: HEK293 Lane 3: Mouse kidney Lane 4: Rat kidney Predicted band size: 22kDa Observed band size: 22kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rpa32-rpa2-rabbit-monoclonal-antibody-m02067-5-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02067-5-rpa2-primary-antibodies-ihc-testing-1.jpgAnti-RPA32/RPA2 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-RPA32/RPA2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02067-5-rpa2-primary-antibodies-ihc-testing-2.jpgAnti-RPA32/RPA2 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-RPA32/RPA2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02067-5-rpa2-primary-antibodies-ihc-testing-3.jpgAnti-RPA32/RPA2 Rabbit Monoclonal AntibodyMouse liver was stained with anti-RPA32/RPA2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02067-5-rpa2-primary-antibodies-ihc-testing-4.jpgAnti-RPA32/RPA2 Rabbit Monoclonal AntibodyRat liver was stained with anti-RPA32/RPA2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02067-5-rpa2-primary-antibodies-wb-testing-1.jpgAnti-RPA32/RPA2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RPA32/RPA2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: HeLa Lane 3: C6 Lane 4: U-14 Predicted band size: 29kDa Observed band size: 29kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytokeratin-19-rabbit-monoclonal-antibody-m02101-8-boster.html2026-03-16T05:09:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02101-8-krt19-primary-antibodies-ihc-testing-1.jpgAnti-Cytokeratin 19 Rabbit Monoclonal AntibodyMouse lung was stained with anti-Cytokeratin 19 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02101-8-krt19-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 19 Rabbit Monoclonal AntibodyRat lung was stained with anti-Cytokeratin 19 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02101-8-krt19-primary-antibodies-ihc-testing-3.jpgAnti-Cytokeratin 19 Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-Cytokeratin 19 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02101-8-krt19-primary-antibodies-ihc-testing-4.jpgAnti-Cytokeratin 19 Rabbit Monoclonal AntibodyHuman lung was stained with anti-Cytokeratin 19 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02101-8-krt19-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 19 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 19 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: 4T1 Predicted band size: 40kDa Observed band size: 40kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psd95-rabbit-monoclonal-antibody-m02128-4-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02128-4-dlg4-primary-antibodies-ihc-testing-1.jpgAnti-PSD95 Rabbit Monoclonal AntibodyHuman brain was stained with anti-PSD95 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02128-4-dlg4-primary-antibodies-wb-testing-1.jpgAnti-PSD95 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PSD95 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Neuro-2a Lane 2: Rat brain Predicted band size: 81kDa Observed band size: 95kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eaat1-rabbit-monoclonal-antibody-m02133-2-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02133-2-slc1a3-primary-antibodies-ihc-testing-1.jpgAnti-EAAT1 Rabbit Monoclonal AntibodyRat brain was stained with Anti-EAAT1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02133-2-slc1a3-primary-antibodies-ihc-testing-2.jpgAnti-EAAT1 Rabbit Monoclonal AntibodyMouse brain was stained with Anti-EAAT1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02133-2-slc1a3-primary-antibodies-ihc-testing-3.jpgAnti-EAAT1 Rabbit Monoclonal AntibodyHuman brain was stained with Anti-EAAT1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02133-2-slc1a3-primary-antibodies-ihc-testing-4.jpgAnti-EAAT1 Rabbit Monoclonal AntibodyHuman glioma was stained with anti-EAAT1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02133-2-slc1a3-primary-antibodies-wb-testing-1.jpgAnti-EAAT1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-EAAT1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-87MG Predicted band size: 60kDa Observed band size: 59kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cdc37-rabbit-monoclonal-antibody-m02169-2-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02169-2-cdc37-primary-antibodies-ihc-testing-1.jpgAnti-CDC37 Rabbit Monoclonal AntibodyHuman brain was stained with Anti-CDC37 (phospho Ser13) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02169-2-cdc37-primary-antibodies-ihc-testing-2.jpgAnti-CDC37 Rabbit Monoclonal AntibodyMouse brain was stained with Anti-CDC37 (phospho Ser13) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02169-2-cdc37-primary-antibodies-ihc-testing-3.jpgAnti-CDC37 Rabbit Monoclonal AntibodyRat brain was stained with Anti-CDC37 (phospho Ser13) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02169-2-cdc37-primary-antibodies-wb-testing-1.jpgAnti-CDC37 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CDC37 (phospho Ser13) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-12 Lane 2: HEK293 Lane 3: HeLa Lane 4: NIH-3T3 Predicted band size: 44kDa Observed band size: 44kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd8-alpha-rabbit-monoclonal-antibody-m02236-5-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02236-5-cd8a-primary-antibodies-ihc-testing-1.jpgAnti-CD8 α Rabbit Monoclonal AntibodyHuman colon was stained with Anti-CD8 α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02236-5-cd8a-primary-antibodies-ihc-testing-2.jpgAnti-CD8 α Rabbit Monoclonal AntibodyRat spleen was stained with Anti-CD8 α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02236-5-cd8a-primary-antibodies-ihc-testing-3.jpgAnti-CD8 α Rabbit Monoclonal AntibodyMouse spleen was stained with Anti-CD8 α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02236-5-cd8a-primary-antibodies-wb-testing-1.jpgAnti-CD8 α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD8 α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse thymus Lane 2: Mouse spleen Lane 3: MOLT-4 Lane 4: Raji Lane 5: SNU-1 Lane 6: HCT-116 Predicted band size: 26kDa Observed band size: 35kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ifitm3-rabbit-monoclonal-antibody-m02265-2-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02265-2-ifitm3-primary-antibodies-ihc-testing-1.jpgAnti-IFITM3 Rabbit Monoclonal AntibodyHuman brain was stained with Anti-IFITM3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02265-2-ifitm3-primary-antibodies-wb-testing-1.jpgAnti-IFITM3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IFITM3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HCT-116 Lane 2: HEK293 Lane 3: HeLa Predicted band size: 14kDa Observed band size: 14kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd206-mrc1-rabbit-monoclonal-antibody-m02285-2-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02285-2-mrc1-primary-antibodies-if-testing-1.jpgAnti-CD206/MRC1 Rabbit Monoclonal AntibodyFluorescence multiplex immunohistochemical analysis of human tonsil tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Sextuple-Fluorescence kit (RS0039, Immunoway).CD3 rabbit mAb(RED), Ki67 rabbit mAb(GREEN), CD31 rabbit mAb(YELLOW), CD206 Rabbit mAb(WHITE) was tested with different TSA Fluorescence regent. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02285-2-mrc1-primary-antibodies-ihc-testing-1.jpgAnti-CD206/MRC1 Rabbit Monoclonal AntibodyHuman colon was stained with anti-CD206/MRC1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02285-2-mrc1-primary-antibodies-ihc-testing-2.jpgAnti-CD206/MRC1 Rabbit Monoclonal AntibodyHuman testis was stained with anti-CD206/MRC1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02285-2-mrc1-primary-antibodies-ihc-testing-3.jpgAnti-CD206/MRC1 Rabbit Monoclonal AntibodyMouse colon was stained with anti-CD206/MRC1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02285-2-mrc1-primary-antibodies-wb-testing-1.jpgAnti-CD206/MRC1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-CD206/MRC1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse liver Predicted band size: 190kDa Observed band size: 190kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eea1-rabbit-monoclonal-antibody-m02296-3-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02296-3-eea1-primary-antibodies-ihc-testing-1.jpgAnti-EEA1 Rabbit Monoclonal AntibodyHuman ductal breast carcinoma was stained with Anti-EEA1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02296-3-eea1-primary-antibodies-wb-testing-1.jpgAnti-EEA1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-EEA1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Predicted band size: 162kDa Observed band size: 162kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02296-3-eea1-primary-antibodies-wb-testing-2.jpgAnti-EEA1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-EEA1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: HeLa Predicted band size: 162kDa Observed band size: 162kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-catenin-d-1-rabbit-monoclonal-antibody-m02333-4-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02333-4-ctnnd1-primary-antibodies-ihc-testing-1.jpgAnti-Catenin δ-1 Rabbit Monoclonal AntibodyRat liver was stained with anti-Catenin δ-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02333-4-ctnnd1-primary-antibodies-ihc-testing-2.jpgAnti-Catenin δ-1 Rabbit Monoclonal AntibodyHuman cervical squamous carcinoma was stained with anti-Catenin δ-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02333-4-ctnnd1-primary-antibodies-ihc-testing-3.jpgAnti-Catenin δ-1 Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-Catenin δ-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02333-4-ctnnd1-primary-antibodies-ihc-testing-4.jpgAnti-Catenin δ-1 Rabbit Monoclonal AntibodyMouse liver was stained with anti-Catenin δ-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02333-4-ctnnd1-primary-antibodies-wb-testing-1.jpgAnti-Catenin δ-1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Catenin δ-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: C6 Lane 3: U-87 MG Predicted band size: 108kDa Observed band size: 108kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pdi-rabbit-monoclonal-antibody-m02335-3-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02335-3-p4hb-primary-antibodies-ihc-testing-1.jpgAnti-PDI Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-PDI rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02335-3-p4hb-primary-antibodies-ihc-testing-2.jpgAnti-PDI Rabbit Monoclonal AntibodyHuman liver was stained with anti-PDI rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02335-3-p4hb-primary-antibodies-ihc-testing-3.jpgAnti-PDI Rabbit Monoclonal AntibodyRat liver was stained with anti-PDI rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02335-3-p4hb-primary-antibodies-wb-testing-1.jpgAnti-PDI Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PDI antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: HCT-116 Lane 3: U-14 Lane 4: Rat womb Predicted band size: 57kDa Observed band size: 57kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp-citrate-lyase-rabbit-monoclonal-antibody-m02372-3-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02372-3-acly-primary-antibodies-ihc-testing-1.jpgAnti-ATP-Citrate Lyase Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-ATP-Citrate Lyase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02372-3-acly-primary-antibodies-wb-testing-1.jpgAnti-ATP-Citrate Lyase Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ATP-Citrate Lyase antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HeLa Lane 3: 3T3-L1 Lane 4: C6 Predicted band size: 121kDa Observed band size: 121kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ferritin-heavy-chain-rabbit-monoclonal-antibody-m02401-3-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02401-3-fth1-primary-antibodies-ihc-testing-1.jpgAnti-Ferritin Heavy Chain Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Ferritin Heavy Chain rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02401-3-fth1-primary-antibodies-ihc-testing-2.jpgAnti-Ferritin Heavy Chain Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Ferritin Heavy Chain rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02401-3-fth1-primary-antibodies-wb-testing-1.jpgAnti-Ferritin Heavy Chain Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Ferritin Heavy Chain antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-14 Lane 2: HeLa Predicted band size: 21kDa Observed band size: 21kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ferritin-heavy-chain-rabbit-monoclonal-antibody-m02401-4-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02401-4-fth1-primary-antibodies-ihc-testing-1.jpgAnti-Ferritin Heavy Chain Rabbit Monoclonal AntibodyMouse kidney was stained with anti-Ferritin Heavy Chain rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02401-4-fth1-primary-antibodies-ihc-testing-2.jpgAnti-Ferritin Heavy Chain Rabbit Monoclonal AntibodyRat kidney was stained with anti-Ferritin Heavy Chain rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02401-4-fth1-primary-antibodies-wb-testing-1.jpgAnti-Ferritin Heavy Chain Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Ferritin Heavy Chain antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse liver Lane 2: Rat liver Predicted band size: 21kDa Observed band size: 21kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rab7-rabbit-monoclonal-antibody-m02409-2-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02409-2-rab7a-primary-antibodies-ihc-testing-1.jpgAnti-Rab7 Rabbit Monoclonal AntibodyHuman liver was stained with anti-Rab7 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02409-2-rab7a-primary-antibodies-ihc-testing-2.jpgAnti-Rab7 Rabbit Monoclonal AntibodyMouse liver was stained with anti-Rab7 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02409-2-rab7a-primary-antibodies-wb-testing-1.jpgAnti-Rab7 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Rab7 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HeLa Lane 3: C6 Lane 4: 3T3-L1 Predicted band size: 24kDa Observed band size: 24kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytokeratin-7-rabbit-monoclonal-antibody-m02416-4-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02416-4-krt7-primary-antibodies-ihc-testing-1.jpgAnti-Cytokeratin 7 Rabbit Monoclonal AntibodyHuman prostate was stained with anti-Cytokeratin 7 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02416-4-krt7-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 7 Rabbit Monoclonal AntibodyMouse liver was stained with anti-Cytokeratin 7 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02416-4-krt7-primary-antibodies-ihc-testing-3.jpgAnti-Cytokeratin 7 Rabbit Monoclonal AntibodyRat liver was stained with anti-Cytokeratin 7 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02416-4-krt7-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 7 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 7 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: A549 Lane 3: Mouse lung Lane 4: Rat lung Predicted band size: 51kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sesn2-rabbit-monoclonal-antibody-m02558-1-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02558-1-sesn2-primary-antibodies-wb-testing-1.jpgAnti-SESN2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SESN2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HEK293 Lane 3: NIH-3T3 Lane 4: PC-12 Predicted band size: 55kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-jak3-rabbit-monoclonal-antibody-m02598-2-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02598-2-jak3-primary-antibodies-wb-testing-1.jpgAnti-JAK3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-JAK3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: KARPAS-299 Lane 2: Jurkat Predicted band size: 125kDa Observed band size: 125kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-jnk1-2-3-rabbit-monoclonal-antibody-m02608-5-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02608-5-mapk8-primary-antibodies-wb-testing-1.jpgAnti-JNK1/2/3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-JNK1/2/3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: K562 Lane 3: C6 Lane 4: 3T3-L1 Predicted band size: 48,53kDa Observed band size: 48,53kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-53bp2-aspp2-rabbit-monoclonal-antibody-m02609-2-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02609-2-tp53bp2-primary-antibodies-wb-testing-1.jpgAnti-53BP2/ASPP2 Rabbit Monoclonal AntibodyWestern Blot analysis of HEK-293T whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-53BP2/ASPP2 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ythdf2-rabbit-monoclonal-antibody-m02621-1-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02621-1-ythdf2-primary-antibodies-ihc-testing-1.jpgAnti-YTHDF2 Rabbit Monoclonal AntibodyMouse testis was stained with anti-YTHDF2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02621-1-ythdf2-primary-antibodies-ihc-testing-2.jpgAnti-YTHDF2 Rabbit Monoclonal AntibodyHuman testis was stained with anti-YTHDF2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02621-1-ythdf2-primary-antibodies-wb-testing-1.jpgAnti-YTHDF2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-YTHDF2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: K562 Lane 3: RAW264.7 Lane 4: C6 Predicted band size: 62kDa Observed band size: 62kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ve-cadherin-rabbit-monoclonal-antibody-m02632-4-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02632-4-cdh5-primary-antibodies-ihc-testing-1.jpgAnti-VE Cadherin Rabbit Monoclonal AntibodyMouse lung was stained with anti-VE Cadherin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02632-4-cdh5-primary-antibodies-ihc-testing-2.jpgAnti-VE Cadherin Rabbit Monoclonal AntibodyRat lung was stained with anti-VE Cadherin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02632-4-cdh5-primary-antibodies-wb-testing-1.jpgAnti-VE Cadherin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-VE Cadherin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse lung Lane 2: Rat lung Predicted band size: 88kDa Observed band size: 90-140kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd3-rabbit-monoclonal-antibody-m02675-10-boster.html2026-03-16T05:09:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02675-10-cd3e-primary-antibodies-ihc-testing-1.jpgAnti-CD3 Rabbit Monoclonal AntibodyRat spleen was stained with anti-CD3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02675-10-cd3e-primary-antibodies-ihc-testing-2.jpgAnti-CD3 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-CD3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02675-10-cd3e-primary-antibodies-ihc-testing-3.jpgAnti-CD3 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-CD3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02675-10-cd3e-primary-antibodies-wb-testing-1.jpgAnti-CD3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: EL-4-B5 Lane 3: Rat thymus Predicted band size: 23kDa Observed band size: 23kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lamin-b1-rabbit-monoclonal-antibody-m02778-1-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02778-1-lmnb1-primary-antibodies-ihc-testing-1.jpgAnti-Lamin B1 Rabbit Monoclonal AntibodyMouse liver was stained with anti-Lamin B1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02778-1-lmnb1-primary-antibodies-ihc-testing-2.jpgAnti-Lamin B1 Rabbit Monoclonal AntibodyRat liver was stained with anti-Lamin B1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02778-1-lmnb1-primary-antibodies-ihc-testing-3.jpgAnti-Lamin B1 Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-Lamin B1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02778-1-lmnb1-primary-antibodies-ihc-testing-4.jpgAnti-Lamin B1 Rabbit Monoclonal AntibodyHuman liver was stained with anti-Lamin B1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02778-1-lmnb1-primary-antibodies-wb-testing-1.jpgAnti-Lamin B1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Lamin B1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: MCF7 Lane 3: NIH-3T3 Lane 4: PC-12 Predicted band size: 66kDa Observed band size: 68kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hp1alpha-rabbit-monoclonal-antibody-m02780-3-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02780-3-cbx5-primary-antibodies-ihc-testing-1.jpgAnti-HP1α Rabbit Monoclonal AntibodyRat pancreas was stained with anti-HP1α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02780-3-cbx5-primary-antibodies-ihc-testing-2.jpgAnti-HP1α Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-HP1α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02780-3-cbx5-primary-antibodies-ihc-testing-3.jpgAnti-HP1α Rabbit Monoclonal AntibodyMouse pancreas was stained with anti-HP1α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02780-3-cbx5-primary-antibodies-wb-testing-1.jpgAnti-HP1α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HP1α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: HeLa Lane 3: U-14 Lane 4: Rat ovary Predicted band size: 22kDa Observed band size: 22kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-erk5-rabbit-monoclonal-antibody-m02812-2-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02812-2-mapk7-primary-antibodies-wb-testing-1.jpgAnti-ERK5 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ERK5 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: NIH-3T3 Lane 3: PC-12 Lane 4: A172 Predicted band size: 88kDa Observed band size: 115kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prostate-specific-membrane-antigen-rabbit-monoclonal-antibody-m02846-9-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02846-9-folh1-primary-antibodies-ihc-testing-1.jpgAnti-Prostate Specific Membrane Antigen Rabbit Monoclonal AntibodyHuman prostate was stained with anti-Prostate Specific Membrane Antigen (PSMA) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02846-9-folh1-primary-antibodies-ihc-testing-2.jpgAnti-Prostate Specific Membrane Antigen Rabbit Monoclonal AntibodyMouse prostate was stained with anti-Prostate Specific Membrane Antigen (PSMA) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02846-9-folh1-primary-antibodies-ihc-testing-3.jpgAnti-Prostate Specific Membrane Antigen Rabbit Monoclonal AntibodyHuman prostate carcinoma was stained with anti-Prostate Specific Membrane Antigen (PSMA) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02846-9-folh1-primary-antibodies-wb-testing-1.jpgAnti-Prostate Specific Membrane Antigen Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Prostate Specific Membrane Antigen (PSMA) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat Prostate Lane 2: SH-SY5Y Predicted band size: 84kDa Observed band size: 84kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ubiquitin-k48-rabbit-monoclonal-antibody-m02848-6-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02848-6-ubiquitin-k48-primary-antibodies-ihc-testing-1.jpgAnti-Ubiquitin K48 Rabbit Monoclonal AntibodyHuman testis was stained with anti-Ubiquitin K48 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02848-6-ubiquitin-k48-primary-antibodies-wb-testing-1.jpgAnti-Ubiquitin K48 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Ubiquitin K48 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HEK293 Lane 2: C6 Lane 3: Jurkat Predicted band size: 26kDa Observed band size: 13-250kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mek3-rabbit-monoclonal-antibody-m02916-5-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02916-5-map2k3-primary-antibodies-wb-testing-1.jpgAnti-MEK3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MEK3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: HeLa Lane 3: 3T3-L1 Lane 4: C6 Predicted band size: 39kDa Observed band size: 39kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pgc1-beta-rabbit-monoclonal-antibody-m02933-1-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02933-1-ppargc1b-primary-antibodies-wb-testing-1.jpgAnti-PGC1 β Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PGC1 β antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MCF7 Lane 2: Mouse spleen Lane 3: SH-SY5Y Lane 4: C6 Predicted band size: 113kDa Observed band size: 113kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atpb-rabbit-monoclonal-antibody-m02937-1-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02937-1-atp5b-primary-antibodies-ihc-testing-1.jpgAnti-ATPB Rabbit Monoclonal AntibodyRat liver was stained with anti-ATPB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02937-1-atp5b-primary-antibodies-ihc-testing-2.jpgAnti-ATPB Rabbit Monoclonal AntibodyHuman kidney was stained with anti-ATPB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02937-1-atp5b-primary-antibodies-ihc-testing-3.jpgAnti-ATPB Rabbit Monoclonal AntibodyHuman liver was stained with anti-ATPB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02937-1-atp5b-primary-antibodies-wb-testing-1.jpgAnti-ATPB Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ATPB antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat spleen Lane 2: Mouse spleen Lane 3: HeLa Lane 4: Raji Predicted band size: 57kDa Observed band size: 52kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gclm-rabbit-monoclonal-antibody-m02948-3-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-3-gclm-primary-antibodies-ihc-testing-1.jpgAnti-GCLM Rabbit Monoclonal AntibodyMouse liver was stained with anti-GCLM rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-3-gclm-primary-antibodies-ihc-testing-2.jpgAnti-GCLM Rabbit Monoclonal AntibodyRat liver was stained with anti-GCLM rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-3-gclm-primary-antibodies-ihc-testing-3.jpgAnti-GCLM Rabbit Monoclonal AntibodyHuman liver was stained with anti-GCLM rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-3-gclm-primary-antibodies-ihc-testing-4.jpgAnti-GCLM Rabbit Monoclonal AntibodyHuman bladder carcinoma was stained with anti-GCLM rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02948-3-gclm-primary-antibodies-wb-testing-1.jpgAnti-GCLM Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GCLM antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: NIH-3T3 Lane 2: PC-12 Lane 3: HeLa Lane 4: MCF7 Predicted band size: 31kDa Observed band size: 31kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd105-rabbit-monoclonal-antibody-m02997-8-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02997-8-eng-primary-antibodies-ihc-testing-1.jpgAnti-CD105 Rabbit Monoclonal AntibodyHuman tonsil was stained with Anti-CD105 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02997-8-eng-primary-antibodies-ihc-testing-2.jpgAnti-CD105 Rabbit Monoclonal AntibodyHuman placenta was stained with anti-CD105 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02997-8-eng-primary-antibodies-wb-testing-1.jpgAnti-CD105 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD105 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: HUVEC Predicted band size: 70kDa Observed band size: 95kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-xct-rabbit-monoclonal-antibody-m03036-2-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-2-slc7a11-primary-antibodies-ihc-testing-1.jpgAnti-xCT Rabbit Monoclonal AntibodyHuman brain was stained with anti-xCT rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-2-slc7a11-primary-antibodies-ihc-testing-2.jpgAnti-xCT Rabbit Monoclonal AntibodyMouse brain was stained with anti-xCT rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-2-slc7a11-primary-antibodies-ihc-testing-3.jpgAnti-xCT Rabbit Monoclonal AntibodyRat brain was stained with anti-xCT rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03036-2-slc7a11-primary-antibodies-wb-testing-1.jpgAnti-xCT Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-xCT antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: unboiled HEK293 Predicted band size: 55kDa Observed band size: 37kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-catalase-rabbit-monoclonal-antibody-m03123-3-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03123-3-cat-primary-antibodies-ihc-testing-1.jpgAnti-Catalase Rabbit Monoclonal AntibodyHuman liver was stained with Anti-Catalase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03123-3-cat-primary-antibodies-ihc-testing-2.jpgAnti-Catalase Rabbit Monoclonal AntibodyMouse liver was stained with anti-Catalase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03123-3-cat-primary-antibodies-ihc-testing-3.jpgAnti-Catalase Rabbit Monoclonal AntibodyRat liver was stained with anti-Catalase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03123-3-cat-primary-antibodies-ihc-testing-4.jpgAnti-Catalase Rabbit Monoclonal AntibodyHuman brain was stained with anti-Catalase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03123-3-cat-primary-antibodies-wb-testing-1.jpgAnti-Catalase Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Catalase antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: HepG2 Lane 3: Jurkat Lane 4: U2OS Lane 5: Rat liver Predicted band size: 60kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-clathrin-heavy-chain-1-rabbit-monoclonal-antibody-m03134-4-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03134-4-ctlc-primary-antibodies-ihc-testing-1.jpgAnti-Clathrin Heavy Chain 1 Rabbit Monoclonal AntibodyMouse colon was stained with anti-Clathrin Heavy Chain 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03134-4-ctlc-primary-antibodies-ihc-testing-2.jpgAnti-Clathrin Heavy Chain 1 Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-Clathrin Heavy Chain 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03134-4-ctlc-primary-antibodies-ihc-testing-3.jpgAnti-Clathrin Heavy Chain 1 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Clathrin Heavy Chain 1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03134-4-ctlc-primary-antibodies-wb-testing-4.jpgAnti-Clathrin Heavy Chain 1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Clathrin Heavy Chain antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-251 MG Predicted band size: 192kDa Observed band size: 180kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03134-4-ctlc-primary-antibodies-wb-testing-5.jpgAnti-Clathrin Heavy Chain 1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Clathrin Heavy Chain 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Mouse brain Predicted band size: 192kDa Observed band size: 180kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03134-4-ctlc-primary-antibodies-wb-testing-6.jpgAnti-Clathrin Heavy Chain 1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Clathrin Heavy Chain 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1:Rat brain Lane 2: U-251 MG Predicted band size: 192kDa Observed band size: 180kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gsk3alpha-rabbit-monoclonal-antibody-m03152-2-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03152-2-gsk3a-primary-antibodies-ihc-testing-1.jpgAnti-GSK3α Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-GSK3α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03152-2-gsk3a-primary-antibodies-wb-testing-1.jpgAnti-GSK3α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GSK3α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HeLa Lane 3: Mouse brain Lane 4: Rat brain Predicted band size: 51kDa Observed band size: 51kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gsk3-alpha-beta-rabbit-monoclonal-antibody-m03152-3-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03152-3-gsk3a-primary-antibodies-ihc-testing-1.jpgAnti-GSK3 α/β Rabbit Monoclonal AntibodyMouse testis was stained with anti-GSK3 α/β rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03152-3-gsk3a-primary-antibodies-ihc-testing-2.jpgAnti-GSK3 α/β Rabbit Monoclonal AntibodyHuman testis was stained with anti-GSK3 α/β rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03152-3-gsk3a-primary-antibodies-wb-testing-1.jpgAnti-GSK3 α/β Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GSK3 α/β antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: HeLa Lane 3: NIH-3T3 Lane 4: C6 Predicted band size: 51,47kDa Observed band size: 51,47kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sptbn1-rabbit-monoclonal-antibody-m03164-1-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03164-1-sptbn1-primary-antibodies-ihc-testing-1.jpgAnti-SPTBN1 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-SPTBN1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03164-1-sptbn1-primary-antibodies-ihc-testing-2.jpgAnti-SPTBN1 Rabbit Monoclonal AntibodyRat kidney was stained with anti-SPTBN1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03164-1-sptbn1-primary-antibodies-wb-testing-3.jpgAnti-SPTBN1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SPTBN1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat brain Predicted band size: 275kDa Observed band size: 275kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03164-1-sptbn1-primary-antibodies-wb-testing-4.jpgAnti-SPTBN1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-SPTBN1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: HeLa Lane 3: Mouse brain Predicted band size: 275kDa Observed band size: 275kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fibrillarin-rabbit-monoclonal-antibody-m03178-6-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03178-6-fbl-primary-antibodies-ihc-testing-1.jpgAnti-Fibrillarin Rabbit Monoclonal AntibodyMouse testis was stained with Anti-Fibrillarin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03178-6-fbl-primary-antibodies-ihc-testing-2.jpgAnti-Fibrillarin Rabbit Monoclonal AntibodyRat liver was stained with anti-Fibrillarin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03178-6-fbl-primary-antibodies-ihc-testing-3.jpgAnti-Fibrillarin Rabbit Monoclonal AntibodyHuman liver was stained with anti-Fibrillarin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03178-6-fbl-primary-antibodies-ihc-testing-4.jpgAnti-Fibrillarin Rabbit Monoclonal AntibodyMouse liver was stained with anti-Fibrillarin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03178-6-fbl-primary-antibodies-wb-testing-1.jpgAnti-Fibrillarin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Fibrillarin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: A172 Lane 3: C6 Lane 4: K562 Predicted band size: 34kDa Observed band size: 34kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glutamine-synthetase-rabbit-monoclonal-antibody-m03191-2-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03191-2-glul-primary-antibodies-ihc-testing-1.jpgAnti-Glutamine Synthetase Rabbit Monoclonal AntibodyRat liver was stained with anti-Glutamine Synthetase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03191-2-glul-primary-antibodies-ihc-testing-2.jpgAnti-Glutamine Synthetase Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-Glutamine Synthetase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03191-2-glul-primary-antibodies-ihc-testing-3.jpgAnti-Glutamine Synthetase Rabbit Monoclonal AntibodyMouse liver was stained with anti-Glutamine Synthetase rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03191-2-glul-primary-antibodies-wb-testing-1.jpgAnti-Glutamine Synthetase Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Glutamine Synthetase antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: Jurkat Lane 3: Rat liver Lane 4: Mouse liver Predicted band size: 42kDa Observed band size: 42kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rictor-rabbit-monoclonal-antibody-m03195-1-boster.html2026-03-16T05:09:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03195-1-rictor-primary-antibodies-ihc-testing-1.jpgAnti-Rictor Rabbit Monoclonal AntibodyMouse lung was stained with anti-Rictor rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03195-1-rictor-primary-antibodies-ihc-testing-2.jpgAnti-Rictor Rabbit Monoclonal AntibodyHuman prostate was stained with anti-Rictor rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03195-1-rictor-primary-antibodies-wb-testing-1.jpgAnti-Rictor Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Rictor antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C2C12 Lane 2: HEK293 Predicted band size: 192kDa Observed band size: 192kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myl2-rabbit-monoclonal-antibody-m03215-2-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03215-2-myl2-primary-antibodies-ihc-testing-1.jpgAnti-MYL2 Rabbit Monoclonal AntibodyRat cardiac muscle was stained with anti-MYL2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03215-2-myl2-primary-antibodies-ihc-testing-2.jpgAnti-MYL2 Rabbit Monoclonal AntibodyHuman skeletal muscle was stained with anti-MYL2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03215-2-myl2-primary-antibodies-ihc-testing-3.jpgAnti-MYL2 Rabbit Monoclonal AntibodyMouse cardiac muscle was stained with anti-MYL2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03215-2-myl2-primary-antibodies-wb-testing-1.jpgAnti-MYL2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MYL2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat heart Lane 2: Mouse heart Predicted band size: 18kDa Observed band size: 18kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-camkii-alpha-rabbit-monoclonal-antibody-m03241-3-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03241-3-camk2a-primary-antibodies-ihc-testing-1.jpgAnti-CaMKII α Rabbit Monoclonal AntibodyHuman brain was stained with anti-CaMKII α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03241-3-camk2a-primary-antibodies-ihc-testing-2.jpgAnti-CaMKII α Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-CaMKII α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03241-3-camk2a-primary-antibodies-ihc-testing-3.jpgAnti-CaMKII α Rabbit Monoclonal AntibodyMouse brain was stained with anti-CaMKII α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03241-3-camk2a-primary-antibodies-wb-testing-1.jpgAnti-CaMKII α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CaMKII α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: Rat brain Lane 3: Mouse brain Predicted band size: 54kDa Observed band size: 54kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mesothelin-rabbit-monoclonal-antibody-m03266-9-boster.html2026-03-16T05:09:25+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trap-rabbit-monoclonal-antibody-m03277-2-boster.html2026-03-16T05:09:25+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fxr1-rabbit-monoclonal-antibody-m03308-1-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03308-1-fxr1-primary-antibodies-ihc-testing-1.jpgAnti-FXR1 Rabbit Monoclonal AntibodyMouse brain was stained with Anti-FXR1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03308-1-fxr1-primary-antibodies-ihc-testing-2.jpgAnti-FXR1 Rabbit Monoclonal AntibodyRat brain was stained with Anti-FXR1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03308-1-fxr1-primary-antibodies-ihc-testing-3.jpgAnti-FXR1 Rabbit Monoclonal AntibodyHuman brain was stained with Anti-FXR1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03308-1-fxr1-primary-antibodies-wb-testing-1.jpgAnti-FXR1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FXR1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat heart Lane 2: HEK293 Lane 3: MCF7 Predicted band size: 70kDa Observed band size: 78-84kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cacna1f-rabbit-monoclonal-antibody-m03442-1-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03442-1-cacna1f-primary-antibodies-wb-testing-1.jpgAnti-CACNA1F Rabbit Monoclonal AntibodyWestern Blot analysis of HL-60 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CACNA1F rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glycogen-synthase-rabbit-monoclonal-antibody-m03512-2-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03512-2-gys1-primary-antibodies-wb-testing-1.jpgAnti-Glycogen Synthase Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Glycogen Synthase 1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: HEK293 Lane 3: C6 Predicted band size: 84kDa Observed band size: 84kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bad-rabbit-monoclonal-antibody-m03520-1-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03520-1-bad-primary-antibodies-ihc-testing-1.jpgAnti-Bad Rabbit Monoclonal AntibodyRat kidney was stained with anti-Bad rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03520-1-bad-primary-antibodies-ihc-testing-2.jpgAnti-Bad Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Bad rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03520-1-bad-primary-antibodies-ihc-testing-3.jpgAnti-Bad Rabbit Monoclonal AntibodyMouse kidney was stained with anti-Bad rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03520-1-bad-primary-antibodies-wb-testing-1.jpgAnti-Bad Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Bad antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: HepG2 Predicted band size: 18kDa Observed band size: 23kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cytochrome-c-rabbit-monoclonal-antibody-m03529-3-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03529-3-cycs-primary-antibodies-ihc-testing-1.jpgAnti-Cytochrome C Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Cytochrome C rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03529-3-cycs-primary-antibodies-ihc-testing-2.jpgAnti-Cytochrome C Rabbit Monoclonal AntibodyHuman liver was stained with anti-Cytochrome C rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03529-3-cycs-primary-antibodies-ihc-testing-3.jpgAnti-Cytochrome C Rabbit Monoclonal AntibodyMouse kidney was stained with anti-Cytochrome C rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03529-3-cycs-primary-antibodies-ihc-testing-4.jpgAnti-Cytochrome C Rabbit Monoclonal AntibodyRat kidney was stained with anti-Cytochrome C rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03529-3-cycs-primary-antibodies-wb-testing-1.jpgAnti-Cytochrome C Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cytochrome C antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Jurkat Lane 2: C6 Lane 3: Rat brain Lane 4: Mouse brain Predicted band size: 12kDa Observed band size: 14kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp5a-rabbit-monoclonal-antibody-m03598-1-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03598-1-atp5a1-primary-antibodies-ihc-testing-1.jpgAnti-ATP5A Rabbit Monoclonal AntibodyHuman kidney was stained with anti-ATP5A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03598-1-atp5a1-primary-antibodies-ihc-testing-2.jpgAnti-ATP5A Rabbit Monoclonal AntibodyHuman liver was stained with anti-ATP5A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03598-1-atp5a1-primary-antibodies-ihc-testing-3.jpgAnti-ATP5A Rabbit Monoclonal AntibodyRat kidney was stained with anti-ATP5A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03598-1-atp5a1-primary-antibodies-wb-testing-1.jpgAnti-ATP5A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ATP5A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: HeLa Lane 3: Rat heart Predicted band size: 60kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pras40-rabbit-monoclonal-antibody-m03629-2-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03629-2-akt1s1-primary-antibodies-wb-testing-1.jpgAnti-PRAS40 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PRAS40 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: C6 Lane 3: HeLa Predicted band size: 27kDa Observed band size: 40kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mtco2-rabbit-monoclonal-antibody-m03631-2-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03631-2-mt-co2-primary-antibodies-ihc-testing-1.jpgAnti-MTCO2 Rabbit Monoclonal AntibodyHuman kidney was stained with Anti-MTCO2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03631-2-mt-co2-primary-antibodies-ihc-testing-2.jpgAnti-MTCO2 Rabbit Monoclonal AntibodyHuman liver was stained with Anti-MTCO2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03631-2-mt-co2-primary-antibodies-wb-testing-1.jpgAnti-MTCO2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MTCO2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: MCF7 Predicted band size: 26kDa Observed band size: 21kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acacb-rabbit-monoclonal-antibody-m03668-1-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03668-1-acacb-primary-antibodies-ihc-testing-1.jpgAnti-ACACB Rabbit Monoclonal AntibodyHuman breast carcinoma was stained with anti-ACACB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03668-1-acacb-primary-antibodies-ihc-testing-2.jpgAnti-ACACB Rabbit Monoclonal AntibodyHuman kidney was stained with anti-ACACB rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03668-1-acacb-primary-antibodies-wb-testing-1.jpgAnti-ACACB Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-ACACB antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: PC-12 Lane 2: NIH-3T3 Lane 3: HEK293 Predicted band size: 227kDa Observed band size: 265kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd20-rabbit-monoclonal-antibody-m03780-18-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03780-18-ms4a1-primary-antibodies-ihc-testing-1.jpgAnti-CD20 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-CD20 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03780-18-ms4a1-primary-antibodies-ihc-testing-2.jpgAnti-CD20 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-CD20 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03780-18-ms4a1-primary-antibodies-wb-testing-1.jpgAnti-CD20 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD20 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Raji Lane 2: Daudi Predicted band size: 33kDa Observed band size: 36kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-synapsin-i-rabbit-monoclonal-antibody-m03794-1-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03794-1-syn1-primary-antibodies-ihc-testing-1.jpgAnti-Synapsin I Rabbit Monoclonal AntibodyHuman brain was stained with anti-Synapsin I rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03794-1-syn1-primary-antibodies-ihc-testing-2.jpgAnti-Synapsin I Rabbit Monoclonal AntibodyMouse brain was stained with anti-Synapsin I rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03794-1-syn1-primary-antibodies-ihc-testing-3.jpgAnti-Synapsin I Rabbit Monoclonal AntibodyRat brain was stained with anti-Synapsin I rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03794-1-syn1-primary-antibodies-wb-testing-1.jpgAnti-Synapsin I Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Synapsin I antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse brain Lane 2: Rat brain Lane 3: SH-SY5Y Predicted band size: 74kDa Observed band size: 74,70kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-itgb6-rabbit-monoclonal-antibody-m03800-1-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03800-1-itgb6-primary-antibodies-ihc-testing-1.jpgAnti-ITGB6 Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-ITGB6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03800-1-itgb6-primary-antibodies-ihc-testing-2.jpgAnti-ITGB6 Rabbit Monoclonal AntibodyMouse testis was stained with anti-ITGB6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03800-1-itgb6-primary-antibodies-ihc-testing-3.jpgAnti-ITGB6 Rabbit Monoclonal AntibodyRat lung was stained with anti-ITGB6 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03800-1-itgb6-primary-antibodies-wb-testing-1.jpgAnti-ITGB6 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ITGB6 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: 3T3-L1 Lane 4: U-87 MG Predicted band size: 86kDa Observed band size: 86kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lamb1-rabbit-monoclonal-antibody-m03894-2-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03894-2-lamb1-primary-antibodies-ihc-testing-1.jpgAnti-LAMB1 Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-LAMB1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03894-2-lamb1-primary-antibodies-wb-testing-1.jpgAnti-LAMB1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti- LAMB1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: U-14 Lane 3: HEK293 Predicted band size: 198kDa Observed band size: 240kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-perilipin-1-rabbit-monoclonal-antibody-m03918-1-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03918-1-plin1-primary-antibodies-ihc-testing-1.jpgAnti-Perilipin-1 Rabbit Monoclonal AntibodyHuman adipose was stained with anti-Perilipin-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03918-1-plin1-primary-antibodies-ihc-testing-2.jpgAnti-Perilipin-1 Rabbit Monoclonal AntibodyMouse adipose was stained with anti-Perilipin-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03918-1-plin1-primary-antibodies-ihc-testing-3.jpgAnti-Perilipin-1 Rabbit Monoclonal AntibodyRat adipose was stained with anti-Perilipin-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03918-1-plin1-primary-antibodies-wb-testing-1.jpgAnti-Perilipin-1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Perilipin-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat fat Lane 2: HepG2 Predicted band size: 56kDa Observed band size: 56kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tagln-rabbit-monoclonal-antibody-m03962-2-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03962-2-tagln-primary-antibodies-ihc-testing-1.jpgAnti-TAGLN Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with anti-TAGLN rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03962-2-tagln-primary-antibodies-ihc-testing-2.jpgAnti-TAGLN Rabbit Monoclonal AntibodyMouse colon was stained with anti-TAGLN rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03962-2-tagln-primary-antibodies-ihc-testing-3.jpgAnti-TAGLN Rabbit Monoclonal AntibodyRat colon was stained with anti-TAGLN rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03962-2-tagln-primary-antibodies-wb-testing-1.jpgAnti-TAGLN Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TAGLN antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat womb Predicted band size: 23kDa Observed band size: 23kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-camkii-beta-rabbit-monoclonal-antibody-m03964-4-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03964-4-camk2b-primary-antibodies-wb-testing-1.jpgAnti-CaMKII β Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CaMKII β antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-87 MG Lane 2: Mouse brain Lane 3: Rat brain Predicted band size: 54kDa Observed band size: 54,60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lyve1-rabbit-monoclonal-antibody-m04027-2-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04027-2-lyve1-primary-antibodies-ihc-testing-1.jpgAnti-LYVE1 Rabbit Monoclonal AntibodyMouse liver was stained with anti-LYVE1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04027-2-lyve1-primary-antibodies-wb-testing-1.jpgAnti-LYVE1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LYVE1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat lung Lane 2: A549 Lane 3: HEK293 Lane 4: Mouse lung Predicted band size: 35kDa Observed band size: 35kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tomm20-rabbit-monoclonal-antibody-m04039-1-boster.html2026-03-16T05:09:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04039-1-tomm20-primary-antibodies-ihc-testing-1.jpgAnti-TOMM20 Rabbit Monoclonal AntibodyHuman cervical carcinoma was stained with anti-TOMM20 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04039-1-tomm20-primary-antibodies-wb-testing-1.jpgAnti-TOMM20 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TOMM20 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: HeLa Lane 3: Mouse liver Lane 4: U-14 Predicted band size: 16kDa Observed band size: 16kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-parvalbumin-rabbit-monoclonal-antibody-m04041-3-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04041-3-pvalb-primary-antibodies-ihc-testing-1.jpgAnti-Parvalbumin Rabbit Monoclonal AntibodyRat brain was stained with anti-Parvalbumin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04041-3-pvalb-primary-antibodies-ihc-testing-2.jpgAnti-Parvalbumin Rabbit Monoclonal AntibodyHuman brain was stained with anti-Parvalbumin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04041-3-pvalb-primary-antibodies-ihc-testing-3.jpgAnti-Parvalbumin Rabbit Monoclonal AntibodyMouse brain was stained with anti-Parvalbumin rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04041-3-pvalb-primary-antibodies-wb-testing-1.jpgAnti-Parvalbumin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Parvalbumin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-251 MG Lane 2: Mouse brain Lane 3: Rat brain Predicted band size: 12kDa Observed band size: 12kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd10-rabbit-monoclonal-antibody-m04065-4-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04065-4-mme-primary-antibodies-ihc-testing-1.jpgAnti-CD10 Rabbit Monoclonal AntibodyRat kidney was stained with anti-CD10 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04065-4-mme-primary-antibodies-ihc-testing-2.jpgAnti-CD10 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-CD10 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04065-4-mme-primary-antibodies-ihc-testing-3.jpgAnti-CD10 Rabbit Monoclonal AntibodyMouse kidney was stained with anti-CD10 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04065-4-mme-primary-antibodies-wb-testing-1.jpgAnti-CD10 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD10 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A172 Lane 2: Romes Lane 3: Mouse kidney Lane 4: Rat kidney Predicted band size: 86kDa Observed band size: 100kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-six2-rabbit-monoclonal-antibody-m04092-1-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04092-1-six2-primary-antibodies-wb-testing-1.jpgAnti-SIX2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SIX2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: RD Lane 2: Neuro-2a Lane 3: Rat brain Predicted band size: 32kDa Observed band size: 38kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cirbp-rabbit-monoclonal-antibody-m04103-1-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04103-1-cirbp-primary-antibodies-ihc-testing-1.jpgAnti-CIRBP Rabbit Monoclonal AntibodyHuman bladder carcinoma was stained with Anti-CIRBP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04103-1-cirbp-primary-antibodies-ihc-testing-2.jpgAnti-CIRBP Rabbit Monoclonal AntibodyHuman colon carcinoma was stained with Anti-CIRBP rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04103-1-cirbp-primary-antibodies-wb-testing-1.jpgAnti-CIRBP Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CIRBP antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: HeLa Predicted band size: 19kDa Observed band size: 19kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acsl4-rabbit-monoclonal-antibody-m04372-2-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04372-2-acsl4-primary-antibodies-ihc-testing-1.jpgAnti-ACSL4 Rabbit Monoclonal AntibodyRat liver was stained with anti-ACSL4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04372-2-acsl4-primary-antibodies-ihc-testing-2.jpgAnti-ACSL4 Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-ACSL4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04372-2-acsl4-primary-antibodies-ihc-testing-3.jpgAnti-ACSL4 Rabbit Monoclonal AntibodyMouse liver was stained with anti-ACSL4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04372-2-acsl4-primary-antibodies-wb-testing-1.jpgAnti-ACSL4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ACSL4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: 3T3-L1 Lane 4: K562 Predicted band size: 78kDa Observed band size: 78kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eif2alpha-rabbit-monoclonal-antibody-m04387-6-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04387-6-eif2s1-primary-antibodies-ihc-testing-1.jpgAnti-eIF2α Rabbit Monoclonal AntibodyHuman breast was stained with anti-eIF2α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04387-6-eif2s1-primary-antibodies-ihc-testing-2.jpgAnti-eIF2α Rabbit Monoclonal AntibodyHuman kidney was stained with anti-eIF2α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04387-6-eif2s1-primary-antibodies-ihc-testing-3.jpgAnti-eIF2α Rabbit Monoclonal AntibodyRat kidney was stained with anti-eIF2α rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04387-6-eif2s1-primary-antibodies-wb-testing-1.jpgAnti-eIF2α Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-eIF2α antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: RAW264.7 Lane 3: HeLa Lane 4: C6 Predicted band size: 36kDa Observed band size: 36kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-oct-2-rabbit-monoclonal-antibody-m04407-3-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04407-3-pou2f2-primary-antibodies-ihc-testing-1.jpgAnti-Oct-2 Rabbit Monoclonal AntibodyRat spleen was stained with anti-Oct-2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04407-3-pou2f2-primary-antibodies-ihc-testing-2.jpgAnti-Oct-2 Rabbit Monoclonal AntibodyHuman spleen was stained with anti-Oct-2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04407-3-pou2f2-primary-antibodies-ihc-testing-3.jpgAnti-Oct-2 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Oct-2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04407-3-pou2f2-primary-antibodies-ihc-testing-4.jpgAnti-Oct-2 Rabbit Monoclonal AntibodyMouse spleen was stained with anti-Oct-2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04407-3-pou2f2-primary-antibodies-wb-testing-1.jpgAnti-Oct-2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Oct-2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Raji Lane 2: Ramos Predicted band size: 51kDa Observed band size: 65kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-flotillin-1-rabbit-monoclonal-antibody-m04517-1-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04517-1-flot1-primary-antibodies-ihc-testing-1.jpgAnti-Flotillin-1 Rabbit Monoclonal AntibodyHumanhepatocellularcarcinoma was stained with anti-Flotillin-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04517-1-flot1-primary-antibodies-ihc-testing-2.jpgAnti-Flotillin-1 Rabbit Monoclonal AntibodyHumancolon was stained with anti-Flotillin-1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04517-1-flot1-primary-antibodies-wb-testing-1.jpgAnti-Flotillin-1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Flotillin-1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: K562 Lane 3: U-14 Lane 4: Rat womb Predicted band size: 47kDa Observed band size: 47kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-caldesmon-rabbit-monoclonal-antibody-m04578-2-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04578-2-cald1-primary-antibodies-ihc-testing-1.jpgAnti-Caldesmon Rabbit Monoclonal AntibodyHuman colon was stained with anti-Caldesmon rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04578-2-cald1-primary-antibodies-ihc-testing-2.jpgAnti-Caldesmon Rabbit Monoclonal AntibodyMouse colon was stained with anti-Caldesmon rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04578-2-cald1-primary-antibodies-ihc-testing-3.jpgAnti-Caldesmon Rabbit Monoclonal AntibodyRat colon was stained with anti-Caldesmon rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04578-2-cald1-primary-antibodies-wb-testing-1.jpgAnti-Caldesmon Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Caldesmon antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: 3T3-L1 Lane 3: C6 Lane 4: U-87 MG Predicted band size: 93kDa Observed band size: 70kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sgsh-rabbit-monoclonal-antibody-m04608-1-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04608-1-sgsh-primary-antibodies-wb-testing-1.jpgAnti-SGSH Rabbit Monoclonal AntibodyWestern Blot analysis of HEK-293 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SGSH rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-puma-rabbit-monoclonal-antibody-m04899-2-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04899-2-bbc3-primary-antibodies-ihc-testing-1.jpgAnti-PUMA Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-PUMA rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04899-2-bbc3-primary-antibodies-wb-testing-1.jpgAnti-PUMA Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PUMA antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: THP-1 Lane 3: C6 Lane 4: 3T3-L1 Predicted band size: 21kDa Observed band size: 21kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-msk1-rabbit-monoclonal-antibody-m04922-1-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04922-1-rps6ka5-primary-antibodies-wb-testing-1.jpgAnti-MSK1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MSK1 (phospho Ser360) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C6 Predicted band size: 90kDa Observed band size: 90kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-neurofilament-heavy-polypeptide-rabbit-monoclonal-antibody-m05307-9-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05307-9-nefh-primary-antibodies-ihc-testing-1.jpgAnti-Neurofilament heavy polypeptide Rabbit Monoclonal AntibodyMouse brain was stained with anti-Neurofilament heavy polypeptide rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05307-9-nefh-primary-antibodies-ihc-testing-2.jpgAnti-Neurofilament heavy polypeptide Rabbit Monoclonal AntibodyRat brain was stained with anti-Neurofilament heavy polypeptide rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05307-9-nefh-primary-antibodies-ihc-testing-3.jpgAnti-Neurofilament heavy polypeptide Rabbit Monoclonal AntibodyHuman brain was stained with anti-Neurofilament heavy polypeptide rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05307-9-nefh-primary-antibodies-wb-testing-4.jpgAnti-Neurofilament heavy polypeptide Rabbit Monoclonal AntibodyWhole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Neurofilament heavy polypeptide antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse brain Predicted band size: 110kDa Observed band size: 200kDa https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05307-9-nefh-primary-antibodies-wb-testing-5.jpgAnti-Neurofilament heavy polypeptide Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Neurofilament heavy polypeptide antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat brain Lane 2: SH-SY5Y Predicted band size: 110kDa Observed band size: 180kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dfna5-gsdme-rabbit-monoclonal-antibody-m05604-1-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05604-1-dfna5-primary-antibodies-wb-testing-1.jpgAnti-DFNA5/GSDME Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DFNA5/GSDME antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-251 MG Lane 2: HepG2 Lane 3: Mouse brain Predicted band size: 55kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-beta-tubulin-rabbit-monoclonal-antibody-m05613-7-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05613-7-tubb-primary-antibodies-wb-testing-1.jpgAnti-β Tubulin Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-β Tubulin antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Rat brain Lane 2: HeLa Lane 3: C6 Lane 4: 3T3-L1 Predicted band size: 50kDa Observed band size: 50kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gm130-rabbit-monoclonal-antibody-m05865-3-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-3-golga2-primary-antibodies-ihc-testing-1.jpgAnti-GM130 Rabbit Monoclonal AntibodyHuman cervical carcinoma was stained with anti-GM130 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-3-golga2-primary-antibodies-ihc-testing-2.jpgAnti-GM130 Rabbit Monoclonal AntibodyHuman colon was stained with anti-GM130 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-3-golga2-primary-antibodies-ihc-testing-3.jpgAnti-GM130 Rabbit Monoclonal AntibodyMouse colon was stained with anti-GM130 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05865-3-golga2-primary-antibodies-wb-testing-1.jpgAnti-GM130 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GM130 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: HepG2 Lane 3: U-14 Lane 4: Rat womb Predicted band size: 113kDa Observed band size: 130kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufs3-rabbit-monoclonal-antibody-m05867-2-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05867-2-ndufs3-primary-antibodies-ihc-testing-1.jpgAnti-NDUFS3 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-NDUFS3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05867-2-ndufs3-primary-antibodies-wb-testing-1.jpgAnti-NDUFS3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NDUFS3 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: K562 Lane 3: Rat brain Predicted band size: 30kDa Observed band size: 30kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-srp72-rabbit-monoclonal-antibody-m05873-1-boster.html2026-03-16T05:09:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05873-1-srp72-primary-antibodies-wb-testing-1.jpgAnti-SRP72 Rabbit Monoclonal AntibodyWestern Blot analysis of HEK-293 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SRP72 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-brat1-rabbit-monoclonal-antibody-m06265-1-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06265-1-brat1-primary-antibodies-wb-testing-1.jpgAnti-BRAT1 Rabbit Monoclonal AntibodyWestern Blot analysis of HeLa whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-BRAT1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-spam1-rabbit-monoclonal-antibody-m06314-1-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06314-1-spam1-primary-antibodies-wb-testing-1.jpgAnti-SPAM1 Rabbit Monoclonal AntibodyWestern Blot analysis of MCF-7 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SPAM1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dohh-rabbit-monoclonal-antibody-m06330-1-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06330-1-dohh-primary-antibodies-wb-testing-1.jpgAnti-DOHH Rabbit Monoclonal AntibodyWestern Blot analysis of HEK-293 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DOHH rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myl9-rabbit-monoclonal-antibody-m06446-2-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06446-2-myl9-primary-antibodies-ihc-testing-1.jpgAnti-MYL9 Rabbit Monoclonal AntibodyMouse colon was stained with anti-MYL9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06446-2-myl9-primary-antibodies-ihc-testing-2.jpgAnti-MYL9 Rabbit Monoclonal AntibodyRat colon was stained with anti-MYL9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06446-2-myl9-primary-antibodies-ihc-testing-3.jpgAnti-MYL9 Rabbit Monoclonal AntibodyHuman colon was stained with anti-MYL9 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06446-2-myl9-primary-antibodies-wb-testing-1.jpgAnti-MYL9 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MYL9 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Rat womb Predicted band size: 20kDa Observed band size: 20kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rbmy1a1-rabbit-monoclonal-antibody-m06728-1-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06728-1-rbmy1a1-primary-antibodies-wb-testing-1.jpgAnti-RBMY1A1 Rabbit Monoclonal AntibodyWestern Blot analysis of COLO 320 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RBMY1A1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ift88-rabbit-monoclonal-antibody-m06814-1-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06814-1-ift88-primary-antibodies-wb-testing-1.jpgAnti-IFT88 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IFT88 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse kidney Lane 2: HEK293 Lane 3: HepG2 Predicted band size: 94kDa Observed band size: 94kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nbeal2-rabbit-monoclonal-antibody-m06878-1-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06878-1-nbeal2-primary-antibodies-wb-testing-1.jpgAnti-NBEAL2 Rabbit Monoclonal AntibodyWestern Blot analysis of HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NBEAL2 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ikb-epsilon-rabbit-monoclonal-antibody-m07073-3-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07073-3-nfkbie-primary-antibodies-wb-testing-1.jpgAnti-IKB epsilon Rabbit Monoclonal AntibodyWestern Blot analysis of A549 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-IKB epsilon rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mob1-rabbit-monoclonal-antibody-m07321-1-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07321-1-mob1a-primary-antibodies-wb-testing-1.jpgAnti-MOB1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MOB1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MOLT-4 Lane 2: C2C12 Lane 3: C6 Lane 4: HeLa Predicted band size: 24kDa Observed band size: 24kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tbr1-rabbit-monoclonal-antibody-m07503-2-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07503-2-tbr1-primary-antibodies-ihc-testing-1.jpgAnti-TBR1 Rabbit Monoclonal AntibodyHuman brain was stained with Anti-TBR1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07503-2-tbr1-primary-antibodies-ihc-testing-2.jpgAnti-TBR1 Rabbit Monoclonal AntibodyMouse brain was stained with Anti-TBR1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07503-2-tbr1-primary-antibodies-wb-testing-1.jpgAnti-TBR1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TBR1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Lane 2: U-251 MG Lane 3: Mouse brain Lane 4: Rat brain Predicted band size: 74kDa Observed band size: 74kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd89-rabbit-monoclonal-antibody-m07546-1-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07546-1-fcar-primary-antibodies-wb-testing-1.jpgAnti-CD89 Rabbit Monoclonal AntibodyWestern Blot analysis of HL-60 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CD89 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pacsin3-rabbit-monoclonal-antibody-m07725-2-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07725-2-pacsin3-primary-antibodies-wb-testing-1.jpgAnti-PACSIN3 Rabbit Monoclonal AntibodyWestern Blot analysis of HeLa whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PACSIN3 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufb8-rabbit-monoclonal-antibody-m07936-2-boster.html2026-03-16T05:09:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07936-2-ndufb8-primary-antibodies-ihc-testing-1.jpgAnti-NDUFB8 Rabbit Monoclonal AntibodyHuman brain was stained with anti-NDUFB8 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07936-2-ndufb8-primary-antibodies-ihc-testing-2.jpgAnti-NDUFB8 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-NDUFB8 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07936-2-ndufb8-primary-antibodies-ihc-testing-3.jpgAnti-NDUFB8 Rabbit Monoclonal AntibodyMouse brain was stained with anti-NDUFB8 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07936-2-ndufb8-primary-antibodies-wb-testing-1.jpgAnti-NDUFB8 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NDUFB8 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Lane 3: RAW264.7 Lane 4: HCT-116 Predicted band size: 22kDa Observed band size: 22kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uqcrc2-rabbit-monoclonal-antibody-m07937-1-boster.html2026-03-16T05:09:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07937-1-uqcrc2-primary-antibodies-ihc-testing-1.jpgAnti-UQCRC2 Rabbit Monoclonal AntibodyRat colon was stained with Anti-UQCRC2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07937-1-uqcrc2-primary-antibodies-ihc-testing-2.jpgAnti-UQCRC2 Rabbit Monoclonal AntibodyMouse colon was stained with Anti-UQCRC2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07937-1-uqcrc2-primary-antibodies-ihc-testing-3.jpgAnti-UQCRC2 Rabbit Monoclonal AntibodyHuman liver was stained with Anti-UQCRC2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07937-1-uqcrc2-primary-antibodies-ihc-testing-4.jpgAnti-UQCRC2 Rabbit Monoclonal AntibodyHuman colon was stained with Anti-UQCRC2 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07937-1-uqcrc2-primary-antibodies-wb-testing-1.jpgAnti-UQCRC2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-UQCRC2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A172 Lane 2: C6 Lane 3: HepG2 Lane 4: Mouse heart Predicted band size: 48kDa Observed band size: 48kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-upb1-rabbit-monoclonal-antibody-m08024-1-boster.html2026-03-16T05:09:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08024-1-upb1-primary-antibodies-wb-testing-1.jpgAnti-UPB1 Rabbit Monoclonal AntibodyWestern Blot analysis of HeLa whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-UPB1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufa1-rabbit-monoclonal-antibody-m08224-2-boster.html2026-03-16T05:09:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08224-2-ndufa1-primary-antibodies-wb-testing-1.jpgAnti-NDUFA1 Rabbit Monoclonal AntibodyWestern Blot analysis of HeLa whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NDUFA1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tuba1b-rabbit-monoclonal-antibody-m08382-7-boster.html2026-03-16T05:09:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08382-7-tuba1b-primary-antibodies-ihc-testing-1.jpgAnti-TUBA1B Rabbit Monoclonal AntibodyRat liver was stained with anti-TUBA1B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08382-7-tuba1b-primary-antibodies-ihc-testing-2.jpgAnti-TUBA1B Rabbit Monoclonal AntibodyHuman liver was stained with anti-TUBA1B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08382-7-tuba1b-primary-antibodies-ihc-testing-3.jpgAnti-TUBA1B Rabbit Monoclonal AntibodyHuman prostate was stained with anti-TUBA1B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08382-7-tuba1b-primary-antibodies-wb-testing-1.jpgAnti-TUBA1B Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TUBA1B antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: HEK293 Lane 3: Mouse kidney Lane 4: Rat kidney Predicted band size: 50kDa Observed band size: 50kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lypd3-rabbit-monoclonal-antibody-m08396-1-boster.html2026-03-16T05:09:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08396-1-lypd3-primary-antibodies-wb-testing-1.jpgAnti-LYPD3 Rabbit Monoclonal AntibodyWestern Blot analysis of HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LYPD3 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-taf1c-rabbit-monoclonal-antibody-m08597-1-boster.html2026-03-16T05:09:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08597-1-taf1c-primary-antibodies-wb-testing-1.jpgAnti-TAF1C Rabbit Monoclonal AntibodyWestern Blot analysis of HT-1080 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TAF1C rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tsfm-rabbit-monoclonal-antibody-m08656-1-boster.html2026-03-16T05:09:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08656-1-tsfm-primary-antibodies-wb-testing-1.jpgAnti-TSFM Rabbit Monoclonal AntibodyWestern Blot analysis of HeLa whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TSFM rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dnph1-rabbit-monoclonal-antibody-m08676-1-boster.html2026-03-16T05:09:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08676-1-dnph1-primary-antibodies-wb-testing-1.jpgAnti-DNPH1 Rabbit Monoclonal AntibodyWestern Blot analysis of K-562 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DNPH1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fam111a-rabbit-monoclonal-antibody-m08753-1-boster.html2026-03-16T05:09:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08753-1-fam111a-primary-antibodies-wb-testing-1.jpgAnti-FAM111A Rabbit Monoclonal AntibodyWestern Blot analysis of HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FAM111A rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cpvl-rabbit-monoclonal-antibody-m09209-1-boster.html2026-03-16T05:09:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09209-1-cpvl-primary-antibodies-wb-testing-1.jpgAnti-CPVL Rabbit Monoclonal AntibodyWestern Blot analysis of HEK-293 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CPVL rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tagap-rabbit-monoclonal-antibody-m09403-1-boster.html2026-03-16T05:09:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09403-1-tagap-primary-antibodies-wb-testing-1.jpgAnti-TAGAP Rabbit Monoclonal AntibodyWestern Blot analysis of HEK-293 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TAGAP rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ppil1-rabbit-monoclonal-antibody-m09483-1-boster.html2026-03-16T05:09:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09483-1-ppil1-primary-antibodies-wb-testing-1.jpgAnti-PPIL1 Rabbit Monoclonal AntibodyWestern Blot analysis of HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PPIL1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ythdf1-rabbit-monoclonal-antibody-m09853-1-boster.html2026-03-16T05:09:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09853-1-ythdf1-primary-antibodies-ihc-testing-1.jpgAnti-YTHDF1 Rabbit Monoclonal AntibodyRat brain was stained with anti-YTHDF1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09853-1-ythdf1-primary-antibodies-ihc-testing-2.jpgAnti-YTHDF1 Rabbit Monoclonal AntibodyHuman brain was stained with anti-YTHDF1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09853-1-ythdf1-primary-antibodies-ihc-testing-3.jpgAnti-YTHDF1 Rabbit Monoclonal AntibodyMouse brain was stained with anti-YTHDF1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09853-1-ythdf1-primary-antibodies-wb-testing-1.jpgAnti-YTHDF1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-YTHDF1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MCF7 Lane 2: NIH-3T3 Lane 3: PC-12 Predicted band size: 61kDa Observed band size: 70kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ptdss2-rabbit-monoclonal-antibody-m09911-1-boster.html2026-03-16T05:09:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09911-1-ptdss2-primary-antibodies-wb-testing-1.jpgAnti-PTDSS2 Rabbit Monoclonal AntibodyWestern Blot analysis of K-562 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PTDSS2 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-poc5-rabbit-monoclonal-antibody-m10282-1-boster.html2026-03-16T05:09:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10282-1-poc5-primary-antibodies-wb-testing-1.jpgAnti-POC5 Rabbit Monoclonal AntibodyWestern Blot analysis of HEK-293 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-POC5 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-map17-rabbit-monoclonal-antibody-m10382-1-boster.html2026-03-16T05:09:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m10382-1-pdzk1ip1-primary-antibodies-wb-testing-1.jpgAnti-Map17 Rabbit Monoclonal AntibodyWestern Blot analysis of HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Map17 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prostein-rabbit-monoclonal-antibody-m11232-1-boster.html2026-03-16T05:09:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11232-1-slc45a3-primary-antibodies-ihc-testing-1.jpgAnti-Prostein Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human Prostate tissue. 1,primary Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tmem192-rabbit-monoclonal-antibody-m11938-1-boster.html2026-03-16T05:09:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11938-1-tmem192-primary-antibodies-wb-testing-1.jpgAnti-TMEM192 Rabbit Monoclonal AntibodyWestern Blot analysis of HeLa whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-TMEM192 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c2orf69-rabbit-monoclonal-antibody-m12291-1-boster.html2026-03-16T05:09:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12291-1-c2orf69-primary-antibodies-wb-testing-1.jpgAnti-C2orf69 Rabbit Monoclonal AntibodyWestern Blot analysis of K-562 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-C2orf69 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h3-rabbit-monoclonal-antibody-m12477-27-boster.html2026-03-16T05:09:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-27-hist1h3a-primary-antibodies-ihc-testing-1.jpgAnti-Histone H3 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Histone H3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-27-hist1h3a-primary-antibodies-ihc-testing-2.jpgAnti-Histone H3 Rabbit Monoclonal AntibodyMouse colon was stained with anti-Histone H3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-27-hist1h3a-primary-antibodies-ihc-testing-3.jpgAnti-Histone H3 Rabbit Monoclonal AntibodyRat colon was stained with anti-Histone H3 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-27-hist1h3a-primary-antibodies-wb-testing-1.jpgAnti-Histone H3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: 3T3-L1 Lane 3: K562 Lane 4: C6 Predicted band size: 15kDa Observed band size: 15kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h3-rabbit-monoclonal-antibody-m12477-28-boster.html2026-03-16T05:09:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-28-hist1h3a-primary-antibodies-ihc-testing-1.jpgAnti-Histone H3 Rabbit Monoclonal AntibodyMouse colon was stained with anti-Histone H3 (Acetyl Lys9) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-28-hist1h3a-primary-antibodies-ihc-testing-2.jpgAnti-Histone H3 Rabbit Monoclonal AntibodyRat colon was stained with anti-Histone H3 (Acetyl Lys9) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-28-hist1h3a-primary-antibodies-ihc-testing-3.jpgAnti-Histone H3 Rabbit Monoclonal AntibodyHuman colon was stained with anti-Histone H3 (Acetyl Lys9) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-28-hist1h3a-primary-antibodies-ihc-testing-4.jpgAnti-Histone H3 Rabbit Monoclonal AntibodyHuman kidney was stained with anti-Histone H3 (Acetyl Lys9) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12477-28-hist1h3a-primary-antibodies-wb-testing-1.jpgAnti-Histone H3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H3 (Acetyl Lys9) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: U-14 Predicted band size: 15kDa Observed band size: 15kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kctd9-rabbit-monoclonal-antibody-m12515-2-boster.html2026-03-16T05:09:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12515-2-kctd9-primary-antibodies-wb-testing-1.jpgAnti-KCTD9 Rabbit Monoclonal AntibodyWestern Blot analysis of HeLa whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-KCTD9 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stard4-rabbit-monoclonal-antibody-m12618-1-boster.html2026-03-16T05:09:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12618-1-stard4-primary-antibodies-wb-testing-1.jpgAnti-STARD4 Rabbit Monoclonal AntibodyWestern Blot analysis of HEK-293 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-STARD4 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-actr1b-rabbit-monoclonal-antibody-m12745-1-boster.html2026-03-16T05:09:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12745-1-actr1b-primary-antibodies-wb-testing-1.jpgAnti-ACTR1B Rabbit Monoclonal AntibodyWestern Blot analysis of HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ACTR1B rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-raver2-rabbit-monoclonal-antibody-m12771-1-boster.html2026-03-16T05:09:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12771-1-raver2-primary-antibodies-wb-testing-1.jpgAnti-RAVER2 Rabbit Monoclonal AntibodyWestern Blot analysis of HeLa whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RAVER2 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phyhd1-rabbit-monoclonal-antibody-m12964-1-boster.html2026-03-16T05:09:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12964-1-phyhd1-primary-antibodies-wb-testing-1.jpgAnti-PHYHD1 Rabbit Monoclonal AntibodyWestern Blot analysis of A549 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PHYHD1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gcet2-rabbit-monoclonal-antibody-m12995-1-boster.html2026-03-16T05:09:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m12995-1-gcsam-primary-antibodies-ihc-testing-1.jpgAnti-GCET2 Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human tonsil tissue. 1,primary Antibody was diluted at 1:200(4°C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fam160a2-rabbit-monoclonal-antibody-m13150-1-boster.html2026-03-16T05:09:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13150-1-fam160a2-primary-antibodies-wb-testing-1.jpgAnti-FAM160A2 Rabbit Monoclonal AntibodyWestern Blot analysis of L02 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-FAM160A2 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dhrs1-rabbit-monoclonal-antibody-m13366-1-boster.html2026-03-16T05:09:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13366-1-dhrs1-primary-antibodies-wb-testing-1.jpgAnti-DHRS1 Rabbit Monoclonal AntibodyWestern Blot analysis of HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DHRS1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nabc1-rabbit-monoclonal-antibody-m13589-1-boster.html2026-03-16T05:09:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13589-1-bcas1-primary-antibodies-wb-testing-1.jpgAnti-NABC1 Rabbit Monoclonal AntibodyWestern Blot analysis of MCF-7 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NABC1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-klhl6-rabbit-monoclonal-antibody-m13962-1-boster.html2026-03-16T05:09:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13962-1-klhl6-primary-antibodies-wb-testing-1.jpgAnti-KLHL6 Rabbit Monoclonal AntibodyWestern Blot analysis of K-562 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-KLHL6 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cshl1-rabbit-monoclonal-antibody-m14242-1-boster.html2026-03-16T05:09:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14242-1-cshl1-primary-antibodies-wb-testing-1.jpgAnti-CSHL1 Rabbit Monoclonal AntibodyWestern Blot analysis of A549 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CSHL1 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kctd3-rabbit-monoclonal-antibody-m14245-1-boster.html2026-03-16T05:09:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14245-1-kctd3-primary-antibodies-wb-testing-1.jpgAnti-KCTD3 Rabbit Monoclonal AntibodyWestern Blot analysis of HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-KCTD3 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dpep3-rabbit-monoclonal-antibody-m14402-1-boster.html2026-03-16T05:09:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14402-1-dpep3-primary-antibodies-wb-testing-1.jpgAnti-DPEP3 Rabbit Monoclonal AntibodyWestern Blot analysis of HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DPEP3 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h4-rabbit-monoclonal-antibody-m14495-15-boster.html2026-03-16T05:09:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14495-15-hist1h4a-primary-antibodies-ihc-testing-1.jpgAnti-Histone H4 Rabbit Monoclonal AntibodyHuman colon was stained with Anti-Histone H4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14495-15-hist1h4a-primary-antibodies-ihc-testing-2.jpgAnti-Histone H4 Rabbit Monoclonal AntibodyMouse kidney was stained with Anti-Histone H4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14495-15-hist1h4a-primary-antibodies-ihc-testing-3.jpgAnti-Histone H4 Rabbit Monoclonal AntibodyRat kidney was stained with Anti-Histone H4 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14495-15-hist1h4a-primary-antibodies-wb-testing-1.jpgAnti-Histone H4 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H4 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: NIH3T3 Lane 3: C6 Predicted band size: 11kDa Observed band size: 11kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-btnl3-rabbit-monoclonal-antibody-m14946-1-boster.html2026-03-16T05:09:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m14946-1-btnl3-primary-antibodies-wb-testing-1.jpgAnti-BTNL3 Rabbit Monoclonal AntibodyWestern Blot analysis of HeLa whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-BTNL3 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h2b-rabbit-monoclonal-antibody-m16595-1-boster.html2026-03-16T05:09:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16595-1-histone-h2b-primary-antibodies-ihc-testing-1.jpgAnti-Histone H2B Rabbit Monoclonal AntibodyRat liver was stained with anti-Histone H2B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16595-1-histone-h2b-primary-antibodies-ihc-testing-2.jpgAnti-Histone H2B Rabbit Monoclonal AntibodyHuman liver was stained with anti-Histone H2B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16595-1-histone-h2b-primary-antibodies-ihc-testing-3.jpgAnti-Histone H2B Rabbit Monoclonal AntibodyMouse liver was stained with anti-Histone H2B rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16595-1-histone-h2b-primary-antibodies-wb-testing-1.jpgAnti-Histone H2B Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H2B antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HeLa Lane 3: C6 Lane 4: 3T3-L1 Predicted band size: 14kDa Observed band size: 14kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slfn12-rabbit-monoclonal-antibody-m16669-1-boster.html2026-03-16T05:09:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16669-1-slfn12-primary-antibodies-wb-testing-1.jpgAnti-SLFN12 Rabbit Monoclonal AntibodyWestern Blot analysis of K-562 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SLFN12 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-histone-h2a-rabbit-monoclonal-antibody-m16777-6-boster.html2026-03-16T05:09:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16777-6-hist1h2ab-primary-antibodies-ihc-testing-1.jpgAnti-Histone H2A Rabbit Monoclonal AntibodyRat liver was stained with anti-Histone H2A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16777-6-hist1h2ab-primary-antibodies-ihc-testing-2.jpgAnti-Histone H2A Rabbit Monoclonal AntibodyRat pancreas was stained with anti-Histone H2A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16777-6-hist1h2ab-primary-antibodies-ihc-testing-3.jpgAnti-Histone H2A Rabbit Monoclonal AntibodyMouse liver was stained with anti-Histone H2A rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16777-6-hist1h2ab-primary-antibodies-wb-testing-1.jpgAnti-Histone H2A Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H2A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: C6 Predicted band size: 14kDa Observed band size: 17kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nup62cl-rabbit-monoclonal-antibody-m16865-1-boster.html2026-03-16T05:09:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m16865-1-nup62cl-primary-antibodies-wb-testing-1.jpgAnti-NUP62CL Rabbit Monoclonal AntibodyWestern Blot analysis of HeLa whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NUP62CL rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cecr5-rabbit-monoclonal-antibody-m17205-1-boster.html2026-03-16T05:09:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m17205-1-cecr5-primary-antibodies-wb-testing-1.jpgAnti-CECR5 Rabbit Monoclonal AntibodyWestern Blot analysis of L02 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CECR5 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c3orf38-rabbit-monoclonal-antibody-m17229-1-boster.html2026-03-16T05:09:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m17229-1-c3orf38-primary-antibodies-wb-testing-1.jpgAnti-C3orf38 Rabbit Monoclonal AntibodyWestern Blot analysis of L02 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-C3orf38 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-actl8-rabbit-monoclonal-antibody-m17253-1-boster.html2026-03-16T05:09:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m17253-1-actl8-primary-antibodies-wb-testing-1.jpgAnti-ACTL8 Rabbit Monoclonal AntibodyWestern Blot analysis of HeLa whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ACTL8 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rbm43-rabbit-monoclonal-antibody-m18243-1-boster.html2026-03-16T05:09:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m18243-1-rbm43-primary-antibodies-wb-testing-1.jpgAnti-RBM43 Rabbit Monoclonal AntibodyWestern Blot analysis of A549 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RBM43 rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mdr1-rabbit-monoclonal-antibody-m30369-1-boster.html2026-03-16T05:09:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30369-1-abcb1a-primary-antibodies-ihc-testing-1.jpgAnti-MDR1 Rabbit Monoclonal AntibodyHuman liver was stained with anti-MDR1 rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30369-1-abcb1a-primary-antibodies-wb-testing-1.jpgAnti-MDR1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-MDR1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: SH-SY5Y Predicted band size: 141kDa Observed band size: 141kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cyclin-b1-rabbit-monoclonal-antibody-m30391-1-boster.html2026-03-16T05:09:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30391-1-ccnb-primary-antibodies-ihc-testing-1.jpgAnti-Cyclin B1 Rabbit Monoclonal AntibodyHuman tonsil was stained with anti-Cyclin B1 (phospho Ser126) rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30391-1-ccnb-primary-antibodies-wb-testing-1.jpgAnti-Cyclin B1 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cyclin B1 (phospho Ser126) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HeLa Lane 2: Jurkat Predicted band size: 48kDa Observed band size: 55kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ly6g-rabbit-monoclonal-antibody-m30404-1-boster.html2026-03-16T05:09:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30404-1-ly6g-primary-antibodies-ihc-testing-1.jpgAnti-Ly6g Rabbit Monoclonal AntibodyMouse spleen was stained with anti-Ly6g rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30404-1-ly6g-primary-antibodies-wb-testing-1.jpgAnti-Ly6g Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Ly6g antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Mouse kindey Predicted band size: 14kDa Observed band size: 14kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gfp-egfp-tag-rabbit-monoclonal-antibody-m30939-3-boster.html2026-03-16T05:09:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m30939-3-gfp-primary-antibodies-wb-testing-1.jpgAnti-GFP/eGFP-Tag Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-GFP/eGFP antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 293F transfection by EGFP Predicted band size: 27kDa Observed band size: 27kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-his-tag-rabbit-monoclonal-antibody-m30975-3-boster.html2026-03-17T05:17:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pparalpha-rabbit-monoclonal-antibody-m31469-1-boster.html2026-03-16T05:09:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m31469-1-piezo-primary-antibodies-ihc-testing-1.jpgAnti-PPARα Rabbit Monoclonal AntibodyRat liver was stained with anti-PPARα rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m31469-1-piezo-primary-antibodies-ihc-testing-2.jpgAnti-PPARα Rabbit Monoclonal AntibodyHuman hepatocellular carcinoma was stained with anti-PPARα rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m31469-1-piezo-primary-antibodies-ihc-testing-3.jpgAnti-PPARα Rabbit Monoclonal AntibodyHuman liver was stained with anti-PPARα rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m31469-1-piezo-primary-antibodies-ihc-testing-4.jpgAnti-PPARα Rabbit Monoclonal AntibodyMouse liver was stained with anti-PPARα rabbit antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m31469-1-piezo-primary-antibodies-wb-testing-1.jpgAnti-PPARα Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PPARα antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2 Lane 2: MCF7 Lane 3: NIH-3T3 Lane 4: L6 Predicted band size: 52kDa Observed band size: 52kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lrmp-rabbit-monoclonal-antibody-m34041-1-boster.html2026-03-16T05:09:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m34041-1-irag2-primary-antibodies-wb-testing-1.jpgAnti-LRMP Rabbit Monoclonal AntibodyWestern Blot analysis of K-562 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LRMP rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-akt-1-2-3-rabbit-monoclonal-antibody-m00024-10-boster.html2026-03-16T05:09:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00024-10-akt1-primary-antibodies-wb-testing-1.jpgAnti-Akt 1/2/3 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-AKT antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1:mouse testis Lane 2:Hela Predicted band size: 60kDa Observed band size: 60kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bcl-2-rabbit-monoclonal-antibody-m00040-5-boster.html2026-03-16T05:09:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00040-5-bcl2-primary-antibodies-wb-testing-1.jpgAnti-BCL-2 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-BCL-2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1:Rat thymus Lane 2:RAW264.7 Lane 3:Jurkat Lane 4:Raji Predicted band size: 26kDa Observed band size: 26kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nf-kb-p65-rabbit-monoclonal-antibody-m00284-3-boster.html2026-03-16T05:09:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00284-3-rela-primary-antibodies-wb-testing-1.jpgAnti-NF-κB p65 Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-NF-κB p65 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1:Hela Lane 2:NIH-3T3 Predicted band size: 65kDa Observed band size: 65kDa https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myeloperoxidaserabbit-monoclonal-antibody-m00372-3-boster.html2026-03-16T05:09:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00372-3-mpo-primary-antibodies-ihc-testing-1.jpgAnti-MyeloperoxidaseRabbit Monoclonal AntibodyHuman spleen tissue was stained with anti-Myeloperoxidase(MPO) rabbit Antibody https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd8-a-rabbit-monoclonal-antibody-m02236-4-boster.html2026-03-16T05:09:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02236-4-cd8-primary-antibodies-ihc-testing-1.jpgAnti-CD8 a Rabbit Monoclonal AntibodyMouse spleen tissue was stained with anti-CD8 a rabbit Antibody https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ha-tag-rabbit-monoclonal-antibody-mt0012-2-boster.html2026-03-16T05:09:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/t/mt0012-2-0-primary-antibodies-wb-testing-1.jpgAnti-HA-Tag Rabbit Monoclonal AntibodyVarious whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-HA-Tag antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HA-MBP(BL21) Predicted band size: 50kDa Observed band size: 55kDa https://www.bosterbio.com/catalog/product/view/id/1309982026-03-10T04:41:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0101.pngMouse IgG ELISA Kit PicoKine® https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-bdnf-antibody-aza0a8m1p735-boster.html2026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1p735-bdnf-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish BDNF Antibody Picoband®Western blot analysis of BDNF using anti-BDNF antibody (AZA0A8M1P735). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDNF antigen affinity purified polyclonal antibody (AZA0A8M1P735) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BDNF at approximately 28 kDa. The expected band size for BDNF is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1p735-bdnf-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish BDNF Antibody Picoband®IHC analysis of BDNF using anti-BDNF antibody (AZA0A8M1P735). BDNF was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BDNF Antibody (AZA0A8M1P735) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1p735-bdnf-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish BDNF Antibody Picoband®IHC analysis of BDNF using anti-BDNF antibody (AZA0A8M1P735). BDNF was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BDNF Antibody (AZA0A8M1P735) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-etv4-antibody-azq9puq1-boster.html2026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9puq1-etv4-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ETV4 Antibody Picoband®Western blot analysis of ETV4 using anti-ETV4 antibody (AZQ9PUQ1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETV4 antigen affinity purified polyclonal antibody (AZQ9PUQ1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ETV4 at approximately 50 kDa. The expected band size for ETV4 is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lhb-antibody-azq6tcf5-boster.html2026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf5-lhb-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish LHB AntibodyIHC analysis of LHB using anti-LHB antibody (AZQ6TCF5). LHB was detected in a paraffin-embedded section of zebrafish hypophysis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHB Antibody (AZQ6TCF5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf5-lhb-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish LHB AntibodyIHC analysis of LHB using anti-LHB antibody (AZQ6TCF5). LHB was detected in a paraffin-embedded section of zebrafish hypothalamus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHB Antibody (AZQ6TCF5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf5-lhb-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish LHB AntibodyIHC analysis of LHB using anti-LHB antibody (AZQ6TCF5). LHB was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHB Antibody (AZQ6TCF5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf5-lhb-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish LHB AntibodyIHC analysis of LHB using anti-LHB antibody (AZQ6TCF5). LHB was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHB Antibody (AZQ6TCF5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf5-lhb-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish LHB AntibodyIHC analysis of LHB using anti-LHB antibody (AZQ6TCF5). LHB was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHB Antibody (AZQ6TCF5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tgfb1a-antibody-azq7zzu7-boster.html2026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zzu7-tgfb1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TGFB1A Antibody Picoband®Western blot analysis of TGFB1A using anti-TGFB1A antibody (AZQ7ZZU7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFB1A antigen affinity purified polyclonal antibody (AZQ7ZZU7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TGFB1A at approximately 40 kDa. The expected band size for TGFB1A is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zzu7-tgfb1a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish TGFB1A Antibody Picoband®IHC analysis of TGFB1A using anti-TGFB1A antibody (AZQ7ZZU7). TGFB1A was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TGFB1A Antibody (AZQ7ZZU7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zzu7-tgfb1a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish TGFB1A Antibody Picoband®IHC analysis of TGFB1A using anti-TGFB1A antibody (AZQ7ZZU7). TGFB1A was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TGFB1A Antibody (AZQ7ZZU7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zzu7-tgfb1a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish TGFB1A Antibody Picoband®IHC analysis of TGFB1A using anti-TGFB1A antibody (AZQ7ZZU7). TGFB1A was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TGFB1A Antibody (AZQ7ZZU7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zzu7-tgfb1a-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish TGFB1A Antibody Picoband®IHC analysis of TGFB1A using anti-TGFB1A antibody (AZQ7ZZU7). TGFB1A was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TGFB1A Antibody (AZQ7ZZU7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hspa5-antibody-azq7szd3-boster.html2026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7szd3-hspa5-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HSPA5 Antibody Picoband®Western blot analysis of HSPA5 using anti-HSPA5 antibody (AZQ7SZD3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA5 antigen affinity purified polyclonal antibody (AZQ7SZD3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HSPA5 at approximately 80 kDa. The expected band size for HSPA5 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7szd3-hspa5-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HSPA5 Antibody Picoband®IHC analysis of HSPA5 using anti-HSPA5 antibody (AZQ7SZD3). HSPA5 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPA5 Antibody (AZQ7SZD3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7szd3-hspa5-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HSPA5 Antibody Picoband®IHC analysis of HSPA5 using anti-HSPA5 antibody (AZQ7SZD3). HSPA5 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPA5 Antibody (AZQ7SZD3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7szd3-hspa5-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HSPA5 Antibody Picoband®IHC analysis of HSPA5 using anti-HSPA5 antibody (AZQ7SZD3). HSPA5 was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPA5 Antibody (AZQ7SZD3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7szd3-hspa5-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish HSPA5 Antibody Picoband®IHC analysis of HSPA5 using anti-HSPA5 antibody (AZQ7SZD3). HSPA5 was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPA5 Antibody (AZQ7SZD3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7szd3-hspa5-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish HSPA5 Antibody Picoband®IHC analysis of HSPA5 using anti-HSPA5 antibody (AZQ7SZD3). HSPA5 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPA5 Antibody (AZQ7SZD3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7szd3-hspa5-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish HSPA5 Antibody Picoband®IHC analysis of HSPA5 using anti-HSPA5 antibody (AZQ7SZD3). HSPA5 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPA5 Antibody (AZQ7SZD3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7szd3-hspa5-primary-antibodies-ihc-testing-7.jpgAnti-Zebrafish HSPA5 Antibody Picoband®IHC analysis of HSPA5 using anti-HSPA5 antibody (AZQ7SZD3). HSPA5 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPA5 Antibody (AZQ7SZD3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fshb-antibody-azq6tcf7-boster.html2026-03-17T05:17:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf7-fshb-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FSHB Antibody Picoband®Western blot analysis of FSHB using anti-FSHB antibody (AZQ6TCF7). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSHB antigen affinity purified polyclonal antibody (AZQ6TCF7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FSHB at approximately 20 kDa. The expected band size for FSHB is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf7-fshb-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish FSHB Antibody Picoband®IHC analysis of FSHB using anti-FSHB antibody (AZQ6TCF7). FSHB was detected in a paraffin-embedded section of zebrafish hypophysis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FSHB Antibody (AZQ6TCF7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf7-fshb-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish FSHB Antibody Picoband®IHC analysis of FSHB using anti-FSHB antibody (AZQ6TCF7). FSHB was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FSHB Antibody (AZQ6TCF7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf7-fshb-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish FSHB Antibody Picoband®IHC analysis of FSHB using anti-FSHB antibody (AZQ6TCF7). FSHB was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FSHB Antibody (AZQ6TCF7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf7-fshb-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish FSHB Antibody Picoband®IHC analysis of FSHB using anti-FSHB antibody (AZQ6TCF7). FSHB was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FSHB Antibody (AZQ6TCF7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-flt1-antibody-azb3dkp1-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb3dkp1-flt1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FLT1 Antibody Picoband®Western blot analysis of FLT1 using anti-FLT1 antibody (AZB3DKP1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLT1 antigen affinity purified polyclonal antibody (AZB3DKP1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FLT1 at approximately 142 kDa. The expected band size for FLT1 is at 142 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb3dkp1-flt1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish FLT1 Antibody Picoband®IHC analysis of FLT1 using anti-FLT1 antibody (AZB3DKP1). FLT1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLT1 Antibody (AZB3DKP1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb3dkp1-flt1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish FLT1 Antibody Picoband®IHC analysis of FLT1 using anti-FLT1 antibody (AZB3DKP1). FLT1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLT1 Antibody (AZB3DKP1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb3dkp1-flt1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish FLT1 Antibody Picoband®IHC analysis of FLT1 using anti-FLT1 antibody (AZB3DKP1). FLT1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLT1 Antibody (AZB3DKP1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb3dkp1-flt1-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish FLT1 Antibody Picoband®IHC analysis of FLT1 using anti-FLT1 antibody (AZB3DKP1). FLT1 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLT1 Antibody (AZB3DKP1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb3dkp1-flt1-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish FLT1 Antibody Picoband®IHC analysis of FLT1 using anti-FLT1 antibody (AZB3DKP1). FLT1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLT1 Antibody (AZB3DKP1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb3dkp1-flt1-primary-antibodies-ihc-testing-6.jpgAnti-Zebrafish FLT1 Antibody Picoband®IHC analysis of FLT1 using anti-FLT1 antibody (AZB3DKP1). FLT1 was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLT1 Antibody (AZB3DKP1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-kita-antibody-azq8jfr5-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8jfr5-kita-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish KITA Antibody Picoband®Western blot analysis of KITA using anti-KITA antibody (AZQ8JFR5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KITA antigen affinity purified polyclonal antibody (AZQ8JFR5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KITA at approximately 95 kDa. The expected band size for KITA is at 109 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8jfr5-kita-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish KITA Antibody Picoband®IHC analysis of KITA using anti-KITA antibody (AZQ8JFR5). KITA was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KITA Antibody (AZQ8JFR5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8jfr5-kita-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish KITA Antibody Picoband®IHC analysis of KITA using anti-KITA antibody (AZQ8JFR5). KITA was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KITA Antibody (AZQ8JFR5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gnrh2-antibody-azq5y835-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5y835-gnrh2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish GNRH2 AntibodyIHC analysis of GNRH2 using anti-GNRH2 antibody (AZQ5Y835). GNRH2 was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNRH2 Antibody (AZQ5Y835) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5y835-gnrh2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GNRH2 AntibodyIHC analysis of GNRH2 using anti-GNRH2 antibody (AZQ5Y835). GNRH2 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNRH2 Antibody (AZQ5Y835) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5y835-gnrh2-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish GNRH2 AntibodyIHC analysis of GNRH2 using anti-GNRH2 antibody (AZQ5Y835). GNRH2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNRH2 Antibody (AZQ5Y835) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gata4-antibody-azq09jy7-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq09jy7-gata4-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GATA4 Antibody Picoband®Western blot analysis of GATA4 using anti-GATA4 antibody (AZQ09JY7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA4 antigen affinity purified polyclonal antibody (AZQ09JY7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GATA4 at approximately 42 kDa. The expected band size for GATA4 is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lhcgr-antibody-azq6tcf8-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf8-lhcgr-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish LHCGR AntibodyIHC analysis of LHCGR using anti-LHCGR antibody (AZQ6TCF8). LHCGR was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHCGR Antibody (AZQ6TCF8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf8-lhcgr-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish LHCGR AntibodyIHC analysis of LHCGR using anti-LHCGR antibody (AZQ6TCF8). LHCGR was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHCGR Antibody (AZQ6TCF8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf8-lhcgr-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish LHCGR AntibodyIHC analysis of LHCGR using anti-LHCGR antibody (AZQ6TCF8). LHCGR was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHCGR Antibody (AZQ6TCF8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf8-lhcgr-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish LHCGR AntibodyIHC analysis of LHCGR using anti-LHCGR antibody (AZQ6TCF8). LHCGR was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHCGR Antibody (AZQ6TCF8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tcf8-lhcgr-primary-antibodies-ihc-testing-5.jpgAnti-Zebrafish LHCGR AntibodyIHC analysis of LHCGR using anti-LHCGR antibody (AZQ6TCF8). LHCGR was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHCGR Antibody (AZQ6TCF8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-her4-1-2-3-5-antibody-azq90466-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90466-her4-1-2-3-5-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HER4(1+2+3+5) AntibodyIHC analysis of HER4(1+2+3+5) using anti-HER4(1+2+3+5) antibody (AZQ90466). HER4(1+2+3+5) was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HER4(1+2+3+5) Antibody (AZQ90466) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90466-her4-1-2-3-5-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HER4(1+2+3+5) AntibodyIHC analysis of HER4(1+2+3+5) using anti-HER4(1+2+3+5) antibody (AZQ90466). HER4(1+2+3+5) was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HER4(1+2+3+5) Antibody (AZQ90466) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mmp13a-antibody-azf1qcx8-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qcx8-mmp13a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MMP13A Antibody Picoband®Western blot analysis of MMP13A using anti-MMP13A antibody (AZF1QCX8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP13A antigen affinity purified polyclonal antibody (AZF1QCX8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MMP13A at approximately 54 kDa. The expected band size for MMP13A is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qcx8-mmp13a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish MMP13A Antibody Picoband®IHC analysis of MMP13A using anti-MMP13A antibody (AZF1QCX8). MMP13A was detected in a paraffin-embedded section of zebrafish spinal column tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP13A Antibody (AZF1QCX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qcx8-mmp13a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish MMP13A Antibody Picoband®IHC analysis of MMP13A using anti-MMP13A antibody (AZF1QCX8). MMP13A was detected in a paraffin-embedded section of zebrafish skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP13A Antibody (AZF1QCX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qcx8-mmp13a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish MMP13A Antibody Picoband®IHC analysis of MMP13A using anti-MMP13A antibody (AZF1QCX8). MMP13A was detected in a paraffin-embedded section of zebrafish spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP13A Antibody (AZF1QCX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tfap2a-antibody-aza0a2r8qrq5-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8qrq5-tfap2a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TFAP2A Antibody Picoband®Western blot analysis of TFAP2A using anti-TFAP2A antibody (AZA0A2R8QRQ5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFAP2A antigen affinity purified polyclonal antibody (AZA0A2R8QRQ5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TFAP2A at approximately 50 kDa. The expected band size for TFAP2A is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gata6-antibody-azq6nw63-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nw63-gata6-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish GATA6 AntibodyIHC analysis of GATA6 using anti-GATA6 antibody (AZQ6NW63). GATA6 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GATA6 Antibody (AZQ6NW63) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gata3-antibody-azq91428-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq91428-gata3-primary-antibodies-wb-testing-1_1.jpgAnti-Zebrafish GATA3 Antibody Picoband®Western blot analysis of GATA3 using anti-GATA3 antibody (AZQ91428). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 antigen affinity purified polyclonal antibody (AZQ91428) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GATA3 at approximately 47 kDa. The expected band size for GATA3 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hmox1a-antibody-azb0uxs0-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0uxs0-hmox1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HMOX1A Antibody Picoband®Western blot analysis of HMOX1A using anti-HMOX1A antibody (AZB0UXS0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMOX1A antigen affinity purified polyclonal antibody (AZB0UXS0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HMOX1A at approximately 30 kDa. The expected band size for HMOX1A is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0uxs0-hmox1a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HMOX1A Antibody Picoband®IHC analysis of HMOX1A using anti-HMOX1A antibody (AZB0UXS0). HMOX1A was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX1A Antibody (AZB0UXS0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0uxs0-hmox1a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HMOX1A Antibody Picoband®IHC analysis of HMOX1A using anti-HMOX1A antibody (AZB0UXS0). HMOX1A was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX1A Antibody (AZB0UXS0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sox3-antibody-azq6ejb7-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6ejb7-sox3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SOX3 Antibody Picoband®Western blot analysis of SOX3 using anti-SOX3 antibody (AZQ6EJB7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX3 antigen affinity purified polyclonal antibody (AZQ6EJB7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SOX3 at approximately 33 kDa. The expected band size for SOX3 is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-stat3-antibody-aza0a8m2bax1-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bax1-stat3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish STAT3 Antibody Picoband®Western blot analysis of STAT3 using anti-STAT3 antibody (AZA0A8M2BAX1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 antigen affinity purified polyclonal antibody (AZA0A8M2BAX1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STAT3 at approximately 91 kDa. The expected band size for STAT3 is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bax1-stat3-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish STAT3 Antibody Picoband®IHC analysis of STAT3 using anti-STAT3 antibody (AZA0A8M2BAX1). STAT3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAT3 Antibody (AZA0A8M2BAX1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bax1-stat3-primary-antibodies-if-testing-1.jpgAnti-Zebrafish STAT3 Antibody Picoband®IF analysis of STAT3 using anti-STAT3 antibody (AZA0A8M2BAX1). STAT3 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-STAT3 Antibody (AZA0A8M2BAX1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-vangl2-antibody-azq8uvj6-boster.html2026-04-06T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8uvj6-vangl2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish VANGL2 Antibody Picoband®Western blot analysis of VANGL2 using anti-VANGL2 antibody (AZQ8UVJ6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VANGL2 antigen affinity purified polyclonal antibody (AZQ8UVJ6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VANGL2 at approximately 60 kDa. The expected band size for VANGL2 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8uvj6-vangl2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish VANGL2 Antibody Picoband®IHC analysis of VANGL2 using anti-VANGL2 antibody (AZQ8UVJ6). VANGL2 was detected in a paraffin-embedded section of zebrafish skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VANGL2 Antibody (AZQ8UVJ6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8uvj6-vangl2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish VANGL2 Antibody Picoband®IHC analysis of VANGL2 using anti-VANGL2 antibody (AZQ8UVJ6). VANGL2 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VANGL2 Antibody (AZQ8UVJ6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-msx1b-antibody-azb2gsg0-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb2gsg0-msx1b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MSX1B Antibody Picoband®Western blot analysis of MSX1B using anti-MSX1B antibody (AZB2GSG0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSX1B antigen affinity purified polyclonal antibody (AZB2GSG0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MSX1B at approximately 28 kDa. The expected band size for MSX1B is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hsd3b1-antibody-azf1qsa2-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qsa2-hsd3b1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HSD3B1 Antibody Picoband®Western blot analysis of HSD3B1 using anti-HSD3B1 antibody (AZF1QSA2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD3B1 antigen affinity purified polyclonal antibody (AZF1QSA2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HSD3B1 at approximately 41 kDa. The expected band size for HSD3B1 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qsa2-hsd3b1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HSD3B1 Antibody Picoband®IHC analysis of HSD3B1 using anti-HSD3B1 antibody (AZF1QSA2). HSD3B1 was detected in a paraffin-embedded section of zebrafish head kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD3B1 Antibody (AZF1QSA2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qsa2-hsd3b1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HSD3B1 Antibody Picoband®IHC analysis of HSD3B1 using anti-HSD3B1 antibody (AZF1QSA2). HSD3B1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD3B1 Antibody (AZF1QSA2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-igf2a-antibody-azq9pud0-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pud0-igf2a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish IGF2A Antibody Picoband®Western blot analysis of IGF2A using anti-IGF2A antibody (AZQ9PUD0). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2A antigen affinity purified polyclonal antibody (AZQ9PUD0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IGF2A at approximately 22 kDa. The expected band size for IGF2A is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pud0-igf2a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish IGF2A Antibody Picoband®IHC analysis of IGF2A using anti-IGF2A antibody (AZQ9PUD0). IGF2A was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF2A Antibody (AZQ9PUD0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pud0-igf2a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish IGF2A Antibody Picoband®IHC analysis of IGF2A using anti-IGF2A antibody (AZQ9PUD0). IGF2A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF2A Antibody (AZQ9PUD0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pud0-igf2a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish IGF2A Antibody Picoband®IHC analysis of IGF2A using anti-IGF2A antibody (AZQ9PUD0). IGF2A was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF2A Antibody (AZQ9PUD0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pax6-a-b-antibody-azp26630-boster.html2026-03-17T05:17:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp26630-pax6-a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PAX6(A+B) Antibody Picoband®Western blot analysis of PAX6(A+B) using anti-PAX6(A+B) antibody (AZP26630). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX6(A+B) antigen affinity purified polyclonal antibody (AZP26630) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAX6(A+B) at approximately 48 kDa. The expected band size for PAX6(A+B) is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp26630-pax6-a-b-primary-antibodies-if-testing-1.jpgAnti-Zebrafish PAX6(A+B) Antibody Picoband®IF analysis of PAX6(A+B) using anti-PAX6(A+B) antibody (AZP26630). PAX6(A+B) was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PAX6(A+B) Antibody (AZP26630) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1310232026-03-10T04:41:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310242026-03-10T04:41:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310252026-03-10T04:41:16+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-c3ar1-antibody.html2026-03-17T05:17:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tsc2-antibody.html2026-03-17T05:17:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310282026-03-10T04:41:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310292026-03-10T04:41:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310302026-03-10T04:41:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310312026-03-10T04:41:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310322026-03-10T04:41:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310332026-03-10T04:41:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310342026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310352026-03-16T05:09:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310362026-03-16T05:09:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310372026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310382026-03-16T05:09:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310392026-03-16T05:09:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310402026-03-16T05:09:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310412026-03-16T05:09:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310422026-03-16T05:09:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310432026-03-16T05:09:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310442026-03-16T05:09:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310452026-03-16T05:09:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310462026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310472026-03-16T05:09:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310482026-03-16T05:09:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310492026-03-16T05:09:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310502026-03-16T05:09:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310512026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310522026-03-16T05:09:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310532026-03-16T05:09:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310542026-03-16T05:09:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310552026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310562026-03-16T05:09:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310572026-03-16T05:09:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310582026-03-16T05:09:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310592026-03-16T05:09:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310602026-03-16T05:09:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310612026-03-16T05:09:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310622026-03-16T05:09:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310632026-03-16T05:09:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310642026-03-16T05:09:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310652026-03-16T05:09:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310662026-03-16T05:09:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310672026-03-16T05:09:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310682026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310692026-03-17T05:17:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10558-1-prrx2-primary-antibodies-wb-testing-1.jpgAnti-PRRX2 Antibody Picoband®Western blot analysis of PRRX2 using anti-PRRX2 antibody (A10558-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRRX2 antigen affinity purified polyclonal antibody (A10558-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRRX2 at approximately 34 kDa. The expected band size for PRRX2 is at 27 kDa. https://www.bosterbio.com/catalog/product/view/id/1310702026-03-16T05:09:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310712026-03-16T05:09:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310722026-03-16T05:09:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310732026-03-16T05:09:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310742026-03-16T05:09:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310752026-03-16T05:09:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310762026-03-16T05:09:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310772026-03-16T05:09:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310782026-03-16T05:09:43+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310792026-03-17T05:17:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15450-psma8-primary-antibodies-wb-testing-1.jpgAnti-PSMA8 Antibody Picoband®Western blot analysis of PSMA8 using anti-PSMA8 antibody (A15450). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA8 antigen affinity purified polyclonal antibody (A15450) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMA8 at approximately 26 kDa. The expected band size for PSMA8 is at 29 kDa. https://www.bosterbio.com/catalog/product/view/id/1310802026-03-16T05:09:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310812026-03-16T05:09:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310822026-03-16T05:09:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310832026-03-16T05:09:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310842026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310852026-03-16T05:09:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310862026-03-16T05:09:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310872026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310882026-03-16T05:09:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310892026-03-16T05:09:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310902026-03-16T05:09:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310912026-03-16T05:09:44+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310922026-03-16T05:09:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310932026-03-16T05:09:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310942026-03-16T05:09:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310952026-03-16T05:09:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310962026-03-16T05:09:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310972026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310982026-03-16T05:09:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1310992026-04-02T05:01:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05295-2-ppp6c-primary-antibodies-wb-testing-1.jpgAnti-PPP6C Antibody Picoband®Western blot analysis of PPP6C using anti-PPP6C antibody (A05295-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP6C antigen affinity purified polyclonal antibody (A05295-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPP6C at approximately 30 kDa. The expected band size for PPP6C is at 35 kDa. https://www.bosterbio.com/catalog/product/view/id/1311002026-03-16T05:09:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311012026-03-16T05:09:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311022026-03-16T05:09:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11369-1-rcan2-primary-antibodies-wb-testing-1.jpgAnti-RCAN2 AntibodyWestern blot analysis of RCAN2 using anti-RCAN2 antibody (A11369-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RCAN2 antigen affinity purified polyclonal antibody (A11369-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RCAN2 at approximately 22 kDa. The expected band size for RCAN2 is at 22 kDa. https://www.bosterbio.com/catalog/product/view/id/1311032026-03-16T05:09:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311042026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311052026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311062026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311072026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311082026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311092026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311102026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311112026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311122026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311132026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311142026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311152026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311162026-03-17T05:17:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04852-1-sec63-primary-antibodies-wb-testing-1.jpgAnti-SEC63 Antibody Picoband®Western blot analysis of SEC63 using anti-SEC63 antibody (A04852-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC63 antigen affinity purified polyclonal antibody (A04852-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SEC63 at approximately 90 kDa. The expected band size for SEC63 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04852-1-sec63-primary-antibodies-ihc-testing-1.jpgAnti-SEC63 Antibody Picoband®IHC analysis of SEC63 using anti-SEC63 antibody (A04852-1). SEC63 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC63 Antibody (A04852-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04852-1-sec63-primary-antibodies-ihc-testing-2.jpgAnti-SEC63 Antibody Picoband®IHC analysis of SEC63 using anti-SEC63 antibody (A04852-1). SEC63 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC63 Antibody (A04852-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04852-1-sec63-primary-antibodies-if-testing-1.jpgAnti-SEC63 Antibody Picoband®IF analysis of SEC63 using anti-SEC63 antibody (A04852-1). SEC63 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SEC63 Antibody (A04852-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04852-1-sec63-primary-antibodies-if-testing-2.jpgAnti-SEC63 Antibody Picoband®IF analysis of SEC63 using anti-SEC63 antibody (A04852-1). SEC63 was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SEC63 Antibody (A04852-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04852-1-sec63-primary-antibodies-fcm-testing-1.jpgAnti-SEC63 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-SEC63 antibody (A04852-1). Overlay histogram showing PC-3 cells stained with A04852-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC63 Antibody (A04852-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1311172026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311182026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311192026-03-16T05:09:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311202026-03-16T05:09:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311212026-03-16T05:09:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311222026-03-16T05:09:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311232026-03-16T05:09:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311242026-03-16T05:09:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311252026-03-16T05:09:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14561-1-cpne2-primary-antibodies-wb-testing-1.jpgAnti-CPNE2 AntibodyWestern blot analysis of CPNE2 using anti-CPNE2 antibody (A14561-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPNE2 antigen affinity purified polyclonal antibody (A14561-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CPNE2 at approximately 61 kDa. The expected band size for CPNE2 is at 61 kDa. https://www.bosterbio.com/catalog/product/view/id/1311262026-03-16T05:09:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311272026-03-16T05:09:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311282026-03-16T05:09:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311292026-03-16T05:09:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311302026-03-16T05:09:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311312026-03-16T05:09:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311322026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311332026-03-16T05:09:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311342026-03-16T05:09:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311352026-03-16T05:09:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311362026-03-16T05:09:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311372026-03-16T05:09:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311382026-03-16T05:09:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311392026-03-16T05:09:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311402026-03-16T05:09:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311412026-03-16T05:09:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311422026-03-16T05:09:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311432026-03-16T05:09:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311442026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311452026-04-04T05:00:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311462026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311472026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311482026-03-16T05:09:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13127-spesp1-primary-antibodies-wb-testing-1.jpgAnti-SPESP1 AntibodyWestern blot analysis of SPESP1 using anti-SPESP1 antibody (A13127). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human OE19 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPESP1 antigen affinity purified polyclonal antibody (A13127) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPESP1 at approximately 77 kDa. The expected band size for SPESP1 is at 39 kDa. https://www.bosterbio.com/catalog/product/view/id/1311492026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311502026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311512026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311522026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311532026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311542026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311552026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311562026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311572026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311582026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311592026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311602026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311612026-03-16T05:09:49+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311622026-03-16T05:09:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311632026-03-16T05:09:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311642026-03-16T05:09:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311652026-03-16T05:09:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311662026-03-16T05:09:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311672026-03-16T05:09:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311682026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311692026-03-16T05:09:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04995-2-klkb1-primary-antibodies-ihc-testing-1.jpgAnti-KLKB1 AntibodyIHC analysis of KLKB1 using anti-KLKB1 antibody (A04995-2). KLKB1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-KLKB1 Antibody (A04995-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04995-2-klkb1-primary-antibodies-ihc-testing-2.jpgAnti-KLKB1 AntibodyIHC analysis of KLKB1 using anti-KLKB1 antibody (A04995-2). KLKB1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-KLKB1 Antibody (A04995-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04995-2-klkb1-primary-antibodies-wb-testing-1.jpgAnti-KLKB1 AntibodyWestern blot analysis of KLKB1 using anti-KLKB1 antibody (A04995-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLKB1 antigen affinity purified polyclonal antibody (A04995-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KLKB1 at approximately 63 kDa. The expected band size for KLKB1 is at 71 kDa. https://www.bosterbio.com/catalog/product/view/id/1311702026-03-16T05:09:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311712026-03-16T05:09:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311722026-03-16T05:09:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311732026-03-17T05:17:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04766-1-sil1-primary-antibodies-wb-testing-1.jpgAnti-SIL1 Antibody Picoband®Western blot analysis of SIL1 using anti-SIL1 antibody (A04766-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIL1 antigen affinity purified polyclonal antibody (A04766-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SIL1 at approximately 52 kDa. The expected band size for SIL1 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04766-1-sil1-primary-antibodies-ihc-testing-1.jpgAnti-SIL1 Antibody Picoband®IHC analysis of SIL1 using anti-SIL1 antibody (A04766-1). SIL1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIL1 Antibody (A04766-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04766-1-sil1-primary-antibodies-ihc-testing-2.jpgAnti-SIL1 Antibody Picoband®IHC analysis of SIL1 using anti-SIL1 antibody (A04766-1). SIL1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIL1 Antibody (A04766-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04766-1-sil1-primary-antibodies-if-testing-1.jpgAnti-SIL1 Antibody Picoband®IF analysis of SIL1 using anti-SIL1 antibody (A04766-1). SIL1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SIL1 Antibody (A04766-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04766-1-sil1-primary-antibodies-fcm-testing-1.jpgAnti-SIL1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-SIL1 antibody (A04766-1). Overlay histogram showing HepG2 cells stained with A04766-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIL1 Antibody (A04766-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1311742026-03-16T05:09:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311752026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311762026-03-16T05:09:50+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311772026-03-16T05:09:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311782026-03-16T05:09:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311792026-03-16T05:09:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311802026-03-16T05:09:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311812026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02748-2-poldip3-primary-antibodies-wb-testing-1.jpgAnti-SKAR/POLDIP3 Antibody Picoband®Western blot analysis of SKAR/POLDIP3 using anti-SKAR/POLDIP3 antibody (A02748-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human CCRF-CEM whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKAR/POLDIP3 antigen affinity purified polyclonal antibody (A02748-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SKAR/POLDIP3 at approximately 46 kDa. The expected band size for SKAR/POLDIP3 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02748-2-poldip3-primary-antibodies-ihc-testing-1.jpgAnti-SKAR/POLDIP3 Antibody Picoband®IHC analysis of SKAR/POLDIP3 using anti-SKAR/POLDIP3 antibody (A02748-2). SKAR/POLDIP3 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SKAR/POLDIP3 Antibody (A02748-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02748-2-poldip3-primary-antibodies-ihc-testing-2.jpgAnti-SKAR/POLDIP3 Antibody Picoband®IHC analysis of SKAR/POLDIP3 using anti-SKAR/POLDIP3 antibody (A02748-2). SKAR/POLDIP3 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SKAR/POLDIP3 Antibody (A02748-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02748-2-poldip3-primary-antibodies-ihc-testing-3.jpgAnti-SKAR/POLDIP3 Antibody Picoband®IHC analysis of SKAR/POLDIP3 using anti-SKAR/POLDIP3 antibody (A02748-2). SKAR/POLDIP3 was detected in a paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SKAR/POLDIP3 Antibody (A02748-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02748-2-poldip3-primary-antibodies-ihc-testing-4.jpgAnti-SKAR/POLDIP3 Antibody Picoband®IHC analysis of SKAR/POLDIP3 using anti-SKAR/POLDIP3 antibody (A02748-2). SKAR/POLDIP3 was detected in a paraffin-embedded section of mouse fallopian tubes tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SKAR/POLDIP3 Antibody (A02748-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02748-2-poldip3-primary-antibodies-ihc-testing-5.jpgAnti-SKAR/POLDIP3 Antibody Picoband®IHC analysis of SKAR/POLDIP3 using anti-SKAR/POLDIP3 antibody (A02748-2). SKAR/POLDIP3 was detected in a paraffin-embedded section of rat fallopian tubes tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SKAR/POLDIP3 Antibody (A02748-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02748-2-poldip3-primary-antibodies-if-testing-1.jpgAnti-SKAR/POLDIP3 Antibody Picoband®IF analysis of SKAR/POLDIP3 using anti-SKAR/POLDIP3 antibody (A02748-2) and anti-Alpha Tubulin antibody (M03989-3). SKAR/POLDIP3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SKAR/POLDIP3 Antibody (A02748-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02748-2-poldip3-primary-antibodies-ip-testing-1.jpgAnti-SKAR/POLDIP3 Antibody Picoband®Immunoprecipitating SKAR/POLDIP3 in Hela whole cell lysate. Western blot analysis of SKAR/POLDIP3 using anti-SKAR/POLDIP3 antibody (A02748-2). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-SKAR/POLDIP3 antibody in Hela whole cell lysate, Lane 3: anti-SKAR/POLDIP3 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SKAR/POLDIP3 antigen affinity purified polyclonal antibody (A02748-2) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SKAR/POLDIP3 at approximately 46 kDa. The expected band size for SKAR/POLDIP3 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02748-2-poldip3-primary-antibodies-fcm-testing-1.jpgAnti-SKAR/POLDIP3 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-SKAR/POLDIP3 antibody (A02748-2). Overlay histogram showing A549 cells stained with A02748-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SKAR/POLDIP3 Antibody (A02748-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1311822026-03-16T05:09:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311832026-03-16T05:09:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311842026-03-17T05:17:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11104-timm9-primary-antibodies-ihc-testing-1.jpgAnti-TIMM9 AntibodyIHC analysis of TIMM9 using anti-TIMM9 antibody (A11104). TIMM9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIMM9 Antibody (A11104) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11104-timm9-primary-antibodies-ihc-testing-2.jpgAnti-TIMM9 AntibodyIHC analysis of TIMM9 using anti-TIMM9 antibody (A11104). TIMM9 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIMM9 Antibody (A11104) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11104-timm9-primary-antibodies-ihc-testing-3.jpgAnti-TIMM9 AntibodyIHC analysis of TIMM9 using anti-TIMM9 antibody (A11104). TIMM9 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIMM9 Antibody (A11104) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11104-timm9-primary-antibodies-ihc-testing-4.jpgAnti-TIMM9 AntibodyIHC analysis of TIMM9 using anti-TIMM9 antibody (A11104). TIMM9 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIMM9 Antibody (A11104) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11104-timm9-primary-antibodies-ihc-testing-5.jpgAnti-TIMM9 AntibodyIHC analysis of TIMM9 using anti-TIMM9 antibody (A11104). TIMM9 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIMM9 Antibody (A11104) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11104-timm9-primary-antibodies-if-testing-1.jpgAnti-TIMM9 AntibodyIF analysis of TIMM9 using anti-TIMM9 antibody (A11104). TIMM9 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TIMM9 Antibody (A11104) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11104-timm9-primary-antibodies-if-testing-2.jpgAnti-TIMM9 AntibodyIF analysis of TIMM9 using anti-TIMM9 antibody (A11104). TIMM9 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TIMM9 Antibody (A11104) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11104-timm9-primary-antibodies-fcm-testing-1.jpgAnti-TIMM9 AntibodyFlow Cytometry analysis of HepG2 cells using anti-TIMM9 antibody (A11104). Overlay histogram showing HepG2 cells stained with A11104 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIMM9 Antibody (A11104, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1311852026-03-16T05:09:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311862026-03-16T05:09:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311872026-03-16T05:09:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311882026-03-16T05:09:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311892026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311902026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311912026-03-17T05:17:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12096-st8sia3-primary-antibodies-wb-testing-1.jpgAnti-ST8SIA3 Antibody Picoband®Western blot analysis of ST8SIA3 using anti-ST8SIA3 antibody (A12096). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST8SIA3 antigen affinity purified polyclonal antibody (A12096) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ST8SIA3 at approximately 44 kDa. The expected band size for ST8SIA3 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12096-st8sia3-primary-antibodies-fcm-testing-1.jpgAnti-ST8SIA3 Antibody Picoband®Flow Cytometry analysis of CACO-2 cells using anti-ST8SIA3 antibody (A12096). Overlay histogram showing CACO-2 cells stained with A12096 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ST8SIA3 Antibody (A12096, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1311922026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311932026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311942026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311952026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311962026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311972026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311982026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1311992026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312002026-03-17T05:17:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07240-1-tns4-primary-antibodies-wb-testing-1.jpgAnti-TNS4 Antibody Picoband®Western blot analysis of TNS4 using anti-TNS4 antibody (A07240-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNS4 antigen affinity purified polyclonal antibody (A07240-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNS4 at approximately 77 kDa. The expected band size for TNS4 is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07240-1-tns4-primary-antibodies-ihc-testing-1.jpgAnti-TNS4 Antibody Picoband®IHC analysis of TNS4 using anti-TNS4 antibody (A07240-1). TNS4 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNS4 Antibody (A07240-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07240-1-tns4-primary-antibodies-ihc-testing-2.jpgAnti-TNS4 Antibody Picoband®IHC analysis of TNS4 using anti-TNS4 antibody (A07240-1). TNS4 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNS4 Antibody (A07240-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07240-1-tns4-primary-antibodies-fcm-testing-1.jpgAnti-TNS4 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-TNS4 antibody (A07240-1). Overlay histogram showing MCF-7 cells stained with A07240-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNS4 Antibody (A07240-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1312012026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312022026-03-17T05:17:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08194-2-slc4a8-primary-antibodies-wb-testing-1.jpgAnti-SLC4A8 Antibody Picoband®Western blot analysis of SLC4A8 using anti-SLC4A8 antibody (A08194-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC4A8 antigen affinity purified polyclonal antibody (A08194-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC4A8 at approximately 150 kDa. The expected band size for SLC4A8 is at 123 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08194-2-slc4a8-primary-antibodies-fcm-testing-1.jpgAnti-SLC4A8 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-SLC4A8 antibody (A08194-2). Overlay histogram showing U251 cells stained with A08194-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC4A8 Antibody (A08194-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1312032026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312042026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312052026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312062026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312072026-03-25T05:25:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05568-2-steap3-primary-antibodies-wb-testing-1.jpgAnti-STEAP3 AntibodyWestern blot analysis of STEAP3 using anti-STEAP3 antibody (A05568-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STEAP3 antigen affinity purified polyclonal antibody (A05568-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STEAP3 at approximately 50 kDa. The expected band size for STEAP3 is at 55 kDa. https://www.bosterbio.com/catalog/product/view/id/1312082026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312092026-03-16T05:09:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312102026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312112026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312122026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312132026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312142026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312152026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312162026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312172026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312182026-03-17T05:17:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07375-2-stk33-primary-antibodies-wb-testing-1.jpgAnti-STK33 Antibody Picoband®Western blot analysis of STK33 using anti-STK33 antibody (A07375-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STK33 antigen affinity purified polyclonal antibody (A07375-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STK33 at approximately 58 kDa. The expected band size for STK33 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07375-2-stk33-primary-antibodies-ihc-testing-1.jpgAnti-STK33 Antibody Picoband®IHC analysis of STK33 using anti-STK33 antibody (A07375-2). STK33 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STK33 Antibody (A07375-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07375-2-stk33-primary-antibodies-if-testing-1.jpgAnti-STK33 Antibody Picoband®IF analysis of STK33 using anti-STK33 antibody (A07375-2) and anti-Beta Tubulin antibody (M01857-3). STK33 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STK33 Antibody (A07375-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07375-2-stk33-primary-antibodies-fcm-testing-1.jpgAnti-STK33 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-STK33 antibody (A07375-2). Overlay histogram showing 293T cells stained with A07375-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STK33 Antibody (A07375-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1312192026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312202026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312212026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312222026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312232026-03-17T05:17:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10511-1-ddost-primary-antibodies-wb-testing-1.jpgAnti-DDOST Antibody Picoband®Western blot analysis of DDOST using anti-DDOST antibody (A10511-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDOST antigen affinity purified polyclonal antibody (A10511-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDOST at approximately 48 kDa. The expected band size for DDOST is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10511-1-ddost-primary-antibodies-ihc-testing-1.jpgAnti-DDOST Antibody Picoband®IHC analysis of DDOST using anti-DDOST antibody (A10511-1). DDOST was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDOST Antibody (A10511-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10511-1-ddost-primary-antibodies-ihc-testing-2.jpgAnti-DDOST Antibody Picoband®IHC analysis of DDOST using anti-DDOST antibody (A10511-1). DDOST was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDOST Antibody (A10511-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10511-1-ddost-primary-antibodies-ip-testing-1.jpgAnti-DDOST Antibody Picoband®Immunoprecipitating DDOST in U251 whole cell lysate. Western blot analysis of DDOST using anti-DDOST antibody (A10511-1). Lane 1: U251 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DDOST antibody in U251 whole cell lysate, Lane 3: anti-DDOST antibody (2μg) + U251 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DDOST antigen affinity purified polyclonal antibody (A10511-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DDOST at approximately 48 kDa. The expected band size for DDOST is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10511-1-ddost-primary-antibodies-fcm-testing-1.jpgAnti-DDOST Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-DDOST antibody (A10511-1). Overlay histogram showing U251 cells stained with A10511-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDOST Antibody (A10511-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1312242026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312252026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312262026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312272026-04-02T05:01:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06954-2-diras3-primary-antibodies-wb-testing-1.jpgAnti-ARHI/DIRAS3 Antibody Picoband®Western blot analysis of ARHI/DIRAS3 using anti-ARHI/DIRAS3 antibody (A06954-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHI/DIRAS3 antigen affinity purified polyclonal antibody (A06954-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ARHI/DIRAS3 at approximately 30 kDa. The expected band size for ARHI/DIRAS3 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06954-2-diras3-primary-antibodies-ihc-testing-1.jpgAnti-ARHI/DIRAS3 Antibody Picoband®IHC analysis of ARHI/DIRAS3 using anti-ARHI/DIRAS3 antibody (A06954-2). ARHI/DIRAS3 was detected in a paraffin-embedded section of mouse fallopian tube tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARHI/DIRAS3 Antibody (A06954-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06954-2-diras3-primary-antibodies-fcm-testing-1.jpgAnti-ARHI/DIRAS3 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-ARHI/DIRAS3 antibody (A06954-2). Overlay histogram showing K562 cells stained with A06954-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ARHI/DIRAS3 Antibody (A06954-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1312282026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312292026-03-16T05:09:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312302026-03-17T05:17:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06160-ufc1-primary-antibodies-wb-testing-1.jpgAnti-UFC1 Antibody Picoband®Western blot analysis of UFC1 using anti-UFC1 antibody (A06160). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UFC1 antigen affinity purified polyclonal antibody (A06160) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UFC1 at approximately 19 kDa. The expected band size for UFC1 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06160-ufc1-primary-antibodies-ip-testing-1.jpgAnti-UFC1 Antibody Picoband®Immunoprecipitating UFC1 in MCF-7 whole cell lysate. Western blot analysis of UFC1 using anti-UFC1 antibody (A06160). Lane 1: MCF-7 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-UFC1 antibody in MCF-7 whole cell lysate, Lane 3: anti-UFC1 antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UFC1 antigen affinity purified polyclonal antibody (A06160) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for UFC1 at approximately 19 kDa. The expected band size for UFC1 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06160-ufc1-primary-antibodies-fcm-testing-1.jpgAnti-UFC1 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-UFC1 antibody (A06160). Overlay histogram showing MCF-7 cells stained with A06160 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UFC1 Antibody (A06160, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1312312026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312322026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312332026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312342026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312352026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312362026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312372026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312382026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312392026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312402026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312412026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312422026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312432026-03-17T05:17:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02962-1-slc23a2-primary-antibodies-wb-testing-1.jpgAnti-SVCT2/SLC23A2 Antibody Picoband®Western blot analysis of SLC23A2 using anti-SLC23A2 antibody (A02962-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human A375 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC23A2 antigen affinity purified polyclonal antibody (A02962-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC23A2 at approximately 75 kDa. The expected band size for SLC23A2 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02962-1-slc23a2-primary-antibodies-if-testing-1.jpgAnti-SVCT2/SLC23A2 Antibody Picoband®IF analysis of SLC23A2 using anti-SLC23A2 antibody (A02962-1). SLC23A2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLC23A2 Antibody (A02962-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02962-1-slc23a2-primary-antibodies-fcm-testing-1.jpgAnti-SVCT2/SLC23A2 Antibody Picoband®Flow Cytometry analysis of CACO-2 cells using anti-SLC23A2 antibody (A02962-1). Overlay histogram showing CACO-2 cells stained with A02962-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC23A2 Antibody (A02962-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1312442026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312452026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312462026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312472026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312482026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312492026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312502026-03-16T05:09:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312512026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312522026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312532026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312542026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312552026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312562026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312572026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13202-2-syt13-primary-antibodies-wb-testing-1.jpgAnti-SYT13 Antibody Picoband®Western blot analysis of SYT13 using anti-SYT13 antibody (A13202-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYT13 antigen affinity purified polyclonal antibody (A13202-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYT13 at approximately 47 kDa. The expected band size for SYT13 is at 47 kDa. https://www.bosterbio.com/catalog/product/view/id/1312582026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312592026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312602026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312612026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312622026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312632026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312642026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312652026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312662026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312672026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312682026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312692026-03-16T05:09:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312702026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312712026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312722026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07376-2-styk1-primary-antibodies-wb-testing-1.jpgAnti-STYK1 Antibody Picoband®Western blot analysis of STYK1 using anti-STYK1 antibody (A07376-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human DLD-1 whole cell lysates, Lane 3: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STYK1 antigen affinity purified polyclonal antibody (A07376-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STYK1 at approximately 48 kDa. The expected band size for STYK1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07376-2-styk1-primary-antibodies-if-testing-1.jpgAnti-STYK1 Antibody Picoband®IF analysis of STYK1 using anti-STYK1 antibody (A07376-2). STYK1 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STYK1 Antibody (A07376-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07376-2-styk1-primary-antibodies-fcm-testing-1.jpgAnti-STYK1 Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-STYK1 antibody (A07376-2). Overlay histogram showing HeLa cells stained with A07376-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-STYK1 Antibody (A07376-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1312732026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312742026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312752026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312762026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312772026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312782026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312792026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312802026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312812026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312822026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312832026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312842026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312852026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312862026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312872026-04-02T05:01:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03732-1-adsl-primary-antibodies-wb-testing-1.jpgAnti-ADSL Antibody Picoband®Western blot analysis of ADSL using anti-ADSL antibody (A03732-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADSL antigen affinity purified polyclonal antibody (A03732-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ADSL at approximately 50 kDa. The expected band size for ADSL is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03732-1-adsl-primary-antibodies-ihc-testing-1.jpgAnti-ADSL Antibody Picoband®IHC analysis of ADSL using anti-ADSL antibody (A03732-1). ADSL was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADSL Antibody (A03732-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03732-1-adsl-primary-antibodies-fcm-testing-1.jpgAnti-ADSL Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-ADSL antibody (A03732-1). Overlay histogram showing 293T cells stained with A03732-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADSL Antibody (A03732-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1312882026-03-13T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00465-2-cyp2c9-primary-antibodies-wb-testing-1.jpgAnti-CYP2C9 AntibodyWestern blot analysis of CYP2C9 using anti-CYP2C9 antibody (A00465-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2C9 antigen affinity purified polyclonal antibody (A00465-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CYP2C9 at approximately 50 kDa. The expected band size for CYP2C9 is at 56 kDa. https://www.bosterbio.com/catalog/product/view/id/1312892026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312902026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312912026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312922026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312932026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02356-3-pik3c3-primary-antibodies-wb-testing-1.jpgAnti-VPS34/PIK3C3 Antibody Picoband®Western blot analysis of VPS34/PIK3C3 using anti-VPS34/PIK3C3 antibody (A02356-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS34/PIK3C3 antigen affinity purified polyclonal antibody (A02356-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VPS34/PIK3C3 at approximately 102 kDa. The expected band size for VPS34/PIK3C3 is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02356-3-pik3c3-primary-antibodies-fcm-testing-1.jpgAnti-VPS34/PIK3C3 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-VPS34/PIK3C3 antibody (A02356-3). Overlay histogram showing SH-SY5Y cells stained with A02356-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VPS34/PIK3C3 Antibody (A02356-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1312942026-03-13T05:05:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312952026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312962026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312972026-03-16T05:09:56+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312982026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1312992026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313002026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313012026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313022026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313032026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313042026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313052026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313062026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313072026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313082026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313092026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313102026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313112026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313122026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313132026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313142026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313152026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313162026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313172026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313182026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313192026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313202026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313212026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313222026-03-16T05:09:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313232026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313242026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313252026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313262026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313272026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313282026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313292026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313302026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313312026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06023-2-itpkb-primary-antibodies-wb-testing-1.jpgAnti-ITPKB Antibody Picoband®Western blot analysis of ITPKB using anti-ITPKB antibody (A06023-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPKB antigen affinity purified polyclonal antibody (A06023-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ITPKB at approximately 150 kDa. The expected band size for ITPKB is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06023-2-itpkb-primary-antibodies-wb-testing-2.jpgAnti-ITPKB Antibody Picoband®Western blot analysis of ITPKB using anti-ITPKB antibody (A06023-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPKB antigen affinity purified polyclonal antibody (A06023-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ITPKB at approximately 102 kDa. The expected band size for ITPKB is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06023-2-itpkb-primary-antibodies-fcm-testing-1.jpgAnti-ITPKB Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-ITPKB antibody (A06023-2). Overlay histogram showing K562 cells stained with A06023-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ITPKB Antibody (A06023-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1313322026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313332026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313342026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313352026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313362026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313372026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02480-dab2ip-primary-antibodies-wb-testing-1.jpgAnti-DAB2IP Antibody Picoband®Western blot analysis of DAB2IP using anti-DAB2IP antibody (A02480). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAB2IP antigen affinity purified polyclonal antibody (A02480) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DAB2IP at approximately 132 kDa. The expected band size for DAB2IP is at 132 kDa. https://www.bosterbio.com/catalog/product/view/id/1313382026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313392026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313402026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313412026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313422026-03-16T05:09:58+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313432026-03-16T05:09:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11553-1-armc8-primary-antibodies-wb-testing-1.jpgAnti-ARMC8 AntibodyWestern blot analysis of ARMC8 using anti-ARMC8 antibody (A11553-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARMC8 antigen affinity purified polyclonal antibody (A11553-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ARMC8 at approximately 76 kDa. The expected band size for ARMC8 is at 76 kDa. https://www.bosterbio.com/catalog/product/view/id/1313442026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313452026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313462026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313472026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17219-1-tmem168-primary-antibodies-wb-testing-1.jpgAnti-TMEM168 AntibodyWestern blot analysis of TMEM168 using anti-TMEM168 antibody (A17219-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM168 antigen affinity purified polyclonal antibody (A17219-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMEM168 at approximately 130 kDa. The expected band size for TMEM168 is at 80 kDa. https://www.bosterbio.com/catalog/product/view/id/1313482026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313492026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313502026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313512026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313522026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02001-1-sftpc-primary-antibodies-wb-testing-1.jpgAnti-SFTPC AntibodyWestern blot analysis of SFTPC using anti-SFTPC antibody (A02001-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPC antigen affinity purified polyclonal antibody (A02001-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SFTPC at approximately 21 kDa. The expected band size for SFTPC is at 21 kDa. https://www.bosterbio.com/catalog/product/view/id/1313532026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313542026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313552026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313562026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313572026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313582026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313592026-03-16T05:09:59+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313602026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313612026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313622026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313632026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313642026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313652026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05799-2-tnnt1-primary-antibodies-ihc-testing-1.jpgAnti-TNNT1 AntibodyIHC analysis of TNNT1 using anti-TNNT1 antibody (A05799-2). TNNT1 was detected in a paraffin-embedded section of mouse skeletal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-TNNT1 Antibody (A05799-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05799-2-tnnt1-primary-antibodies-ihc-testing-2.jpgAnti-TNNT1 AntibodyIHC analysis of TNNT1 using anti-TNNT1 antibody (A05799-2). TNNT1 was detected in a paraffin-embedded section of rat skeletal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-TNNT1 Antibody (A05799-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1313662026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313672026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313682026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313692026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313702026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313712026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02405-2-dclre1c-primary-antibodies-wb-testing-1.jpgAnti-DCLRE1C Antibody Picoband®Western blot analysis of DCLRE1C using anti-DCLRE1C antibody (A02405-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human HEL whole cell lysates. Lane 5: rat RH35 whole cell lysates. Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCLRE1C antigen affinity purified polyclonal antibody (A02405-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCLRE1C at approximately 90-100 kDa. The expected band size for DCLRE1C is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02405-2-dclre1c-primary-antibodies-if-testing-1.jpgAnti-DCLRE1C Antibody Picoband®IF analysis of DCLRE1C using anti-DCLRE1C antibody (A02405-2) and anti-Alpha Tubulin antibody (M03989-3). DCLRE1C was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DCLRE1C Antibody (A02405-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1313722026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313732026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313742026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313752026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313762026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07775-1-slc32a1-primary-antibodies-wb-testing-1.jpgAnti-VGAT/SLC32A1 AntibodyWestern blot analysis of VGAT/SLC32A1 using anti-VGAT/SLC32A1 antibody (A07775-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VGAT/SLC32A1 antigen affinity purified polyclonal antibody (A07775-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VGAT/SLC32A1 at approximately 57 kDa. The expected band size for VGAT/SLC32A1 is at 57 kDa. https://www.bosterbio.com/catalog/product/view/id/1313772026-03-16T05:10:00+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313782026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313792026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313802026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313812026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313822026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04344-2-ttf2-primary-antibodies-wb-testing-1.jpgAnti-TTF2 Antibody Picoband®Western blot analysis of TTF2 using anti-TTF2 antibody (A04344-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTF2 antigen affinity purified polyclonal antibody (A04344-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TTF2 at approximately 130 kDa. The expected band size for TTF2 is at 130 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04344-2-ttf2-primary-antibodies-ihc-testing-1.jpgAnti-TTF2 Antibody Picoband®IHC analysis of TTF2 using anti-TTF2 antibody (A04344-2). TTF2 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTF2 Antibody (A04344-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04344-2-ttf2-primary-antibodies-ihc-testing-2.jpgAnti-TTF2 Antibody Picoband®IHC analysis of TTF2 using anti-TTF2 antibody (A04344-2). TTF2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTF2 Antibody (A04344-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04344-2-ttf2-primary-antibodies-ihc-testing-3.jpgAnti-TTF2 Antibody Picoband®IHC analysis of TTF2 using anti-TTF2 antibody (A04344-2). TTF2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTF2 Antibody (A04344-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04344-2-ttf2-primary-antibodies-if-testing-1.jpgAnti-TTF2 Antibody Picoband®IF analysis of TTF2 using anti-TTF2 antibody (A04344-2). TTF2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TTF2 Antibody (A04344-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04344-2-ttf2-primary-antibodies-fcm-testing-1.jpgAnti-TTF2 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-TTF2 antibody (A04344-2). Overlay histogram showing Jurkat cells stained with A04344-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTF2 Antibody (A04344-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1313832026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313842026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313852026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313862026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313872026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313882026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313892026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313902026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313912026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313922026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313932026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313942026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313952026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12204-3-ddx52-primary-antibodies-wb-testing-1.jpgAnti-DDX52 Antibody Picoband®Western blot analysis of DDX52 using anti-DDX52 antibody (A12204-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX52 antigen affinity purified polyclonal antibody (A12204-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX52 at approximately 67 kDa. The expected band size for DDX52 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12204-3-ddx52-primary-antibodies-ihc-testing-1.jpgAnti-DDX52 Antibody Picoband®IHC analysis of DDX52 using anti-DDX52 antibody (A12204-3). DDX52 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX52 Antibody (A12204-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12204-3-ddx52-primary-antibodies-ihc-testing-2.jpgAnti-DDX52 Antibody Picoband®IHC analysis of DDX52 using anti-DDX52 antibody (A12204-3). DDX52 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX52 Antibody (A12204-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12204-3-ddx52-primary-antibodies-ihc-testing-3.jpgAnti-DDX52 Antibody Picoband®IHC analysis of DDX52 using anti-DDX52 antibody (A12204-3). DDX52 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX52 Antibody (A12204-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12204-3-ddx52-primary-antibodies-ihc-testing-4.jpgAnti-DDX52 Antibody Picoband®IHC analysis of DDX52 using anti-DDX52 antibody (A12204-3). DDX52 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX52 Antibody (A12204-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12204-3-ddx52-primary-antibodies-ihc-testing-5.jpgAnti-DDX52 Antibody Picoband®IHC analysis of DDX52 using anti-DDX52 antibody (A12204-3). DDX52 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX52 Antibody (A12204-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12204-3-ddx52-primary-antibodies-ihc-testing-6.jpgAnti-DDX52 Antibody Picoband®IHC analysis of DDX52 using anti-DDX52 antibody (A12204-3). DDX52 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX52 Antibody (A12204-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12204-3-ddx52-primary-antibodies-if-testing-1.jpgAnti-DDX52 Antibody Picoband®IF analysis of DDX52 using anti-DDX52 antibody (A12204-3) and anti-Alpha Tubulin antibody (M03989-3). DDX52 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDX52 Antibody (A12204-3) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12204-3-ddx52-primary-antibodies-fcm-testing-1.jpgAnti-DDX52 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-DDX52 antibody (A12204-3). Overlay histogram showing A549 cells stained with A12204-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX52 Antibody (A12204-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1313962026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313972026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313982026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1313992026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314002026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10014-2-pgam2-primary-antibodies-wb-testing-1.jpgAnti-PGAM2 Antibody Picoband®Western blot analysis of PGAM2 using anti-PGAM2 antibody (A10014-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGAM2 antigen affinity purified polyclonal antibody (A10014-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PGAM2 at approximately 29 kDa. The expected band size for PGAM2 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10014-2-pgam2-primary-antibodies-ihc-testing-1.jpgAnti-PGAM2 Antibody Picoband®IHC analysis of PGAM2 using anti-PGAM2 antibody (A10014-2). PGAM2 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGAM2 Antibody (A10014-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10014-2-pgam2-primary-antibodies-if-testing-1.jpgAnti-PGAM2 Antibody Picoband®IF analysis of PGAM2 using anti-PGAM2 antibody (A10014-2). PGAM2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PGAM2 Antibody (A10014-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10014-2-pgam2-primary-antibodies-fcm-testing-1.jpgAnti-PGAM2 Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-PGAM2 antibody (A10014-2). Overlay histogram showing HeLa cells stained with A10014-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PGAM2 Antibody (A10014-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1314012026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314022026-03-16T05:10:01+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314032026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314042026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314052026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314062026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314072026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314082026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314092026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314102026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314112026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314122026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314132026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314142026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314152026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314162026-03-13T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02635-1-actn1-primary-antibodies-wb-testing-1.jpgAnti-Alpha Actinin/ACTN1 AntibodyWestern blot analysis of ACTN1 using anti-ACTN1 antibody (A02635-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTN1 antigen affinity purified polyclonal antibody (A02635-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ACTN1 at approximately 105 kDa. The expected band size for ACTN1 is at 103 kDa. https://www.bosterbio.com/catalog/product/view/id/1314172026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314182026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03706-1-elf2-primary-antibodies-wb-testing-1.jpgAnti-ELF2 Antibody Picoband®Western blot analysis of ELF2 using anti-ELF2 antibody (A03706-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Reh whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELF2 antigen affinity purified polyclonal antibody (A03706-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ELF2 at approximately 57 kDa. The expected band size for ELF2 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03706-1-elf2-primary-antibodies-fcm-testing-1.jpgAnti-ELF2 Antibody Picoband®Flow Cytometry analysis of A2780 cells using anti-ELF2 antibody (A03706-1). Overlay histogram showing A2780 cells stained with A03706-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ELF2 Antibody (A03706-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1314192026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314202026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314212026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314222026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314232026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314242026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314252026-03-16T05:10:02+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314262026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31676-2-cgas-primary-antibodies-wb-testing-1.jpgAnti-cGAS AntibodyWestern blot analysis of cGAS using anti-cGAS antibody (A31676-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cGAS antigen affinity purified polyclonal antibody (A31676-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for cGAS at approximately 59-65 kDa. The expected band size for cGAS is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31676-2-cgas-primary-antibodies-wb-testing-2.jpgAnti-cGAS AntibodyWestern blot analysis of cGAS using anti-cGAS antibody (A31676-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cGAS antigen affinity purified polyclonal antibody (A31676-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for cGAS at approximately 59-65 kDa. The expected band size for cGAS is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31676-2-cgas-primary-antibodies-ihc-testing-1.jpgAnti-cGAS AntibodyIHC analysis of cGAS using anti-cGAS antibody (A31676-2). cGAS was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-cGAS Antibody (A31676-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31676-2-cgas-primary-antibodies-ihc-testing-2.jpgAnti-cGAS AntibodyIHC analysis of cGAS using anti-cGAS antibody (A31676-2). cGAS was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-cGAS Antibody (A31676-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1314272026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314282026-03-16T05:10:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13263-2-col23a1-primary-antibodies-wb-testing-1.jpgAnti-COL23A1 AntibodyWestern blot analysis of COL23A1 using anti-COL23A1 antibody (A13263-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL23A1 antigen affinity purified polyclonal antibody (A13263-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for COL23A1 at approximately 70 kDa. The expected band size for COL23A1 is at 52 kDa. https://www.bosterbio.com/catalog/product/view/id/1314292026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314302026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04746-3-slc25a12-primary-antibodies-wb-testing-1.jpgAnti-SLC25A12 Antibody Picoband®Western blot analysis of SLC25A12 using anti-SLC25A12 antibody (A04746-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse skeletal muscle tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A12 antigen affinity purified polyclonal antibody (A04746-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A12 at approximately 75 kDa. The expected band size for SLC25A12 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04746-3-slc25a12-primary-antibodies-if-testing-1.jpgAnti-SLC25A12 Antibody Picoband®IF analysis of SLC25A12 using anti-SLC25A12 antibody (A04746-3) and anti-Alpha Tubulin antibody (M03989-3). SLC25A12 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLC25A12 Antibody (A04746-3) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04746-3-slc25a12-primary-antibodies-fcm-testing-1.jpgAnti-SLC25A12 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-SLC25A12 antibody (A04746-3). Overlay histogram showing HEL cells stained with A04746-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC25A12 Antibody (A04746-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1314312026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314322026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314332026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314342026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08060-3-ldhc-primary-antibodies-wb-testing-1.jpgAnti-LDHC AntibodyWestern blot analysis of LDHC using anti-LDHC antibody (A08060-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDHC antigen affinity purified polyclonal antibody (A08060-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LDHC at approximately 35 kDa. The expected band size for LDHC is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08060-3-ldhc-primary-antibodies-ihc-testing-1.jpgAnti-LDHC AntibodyIHC analysis of LDHC using anti-LDHC antibody (A08060-3). LDHC was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-LDHC Antibody (A08060-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1314352026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314362026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314372026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314382026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314392026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314402026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314412026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314422026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314432026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314442026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314452026-03-13T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02373-1-cgn-primary-antibodies-wb-testing-1.jpgAnti-Cingulin/CGN AntibodyWestern blot analysis of Cingulin/CGN using anti-Cingulin/CGN antibody (A02373-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cingulin/CGN antigen affinity purified polyclonal antibody (A02373-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cingulin/CGN at approximately 150 kDa. The expected band size for Cingulin/CGN is at 136 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02373-1-cgn-primary-antibodies-wb-testing-1.pngAnti-Cingulin/CGN AntibodyWestern blot analysis of Cingulin/CGN using anti-Cingulin/CGN antibody (A02373-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: normal group-rat colon tissue lysates, Lane 2: control group-model rat colon tissue lysates, Lane 3: low dose Chinese medicine group-model rat colon tissue lysates, Lane 4: medium dose Chinese medicine group-model rat colon tissue lysates, Lane 5: high dose Chinese medicine group-model rat colon tissue lysates, Lane 6: western medicine group-model rat colon tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cingulin/CGN antigen affinity purified polyclonal antibody (A02373-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. The expected band size for Cingulin/CGN is at 136 kDa. https://www.bosterbio.com/catalog/product/view/id/1314462026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314472026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05638-1-col8a1-primary-antibodies-wb-testing-1.jpgAnti-COL8A1 AntibodyWestern blot analysis of COL8A1 using anti-COL8A1 antibody (A05638-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL8A1 antigen affinity purified polyclonal antibody (A05638-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for COL8A1 at approximately 73 kDa. The expected band size for COL8A1 is at 73 kDa. https://www.bosterbio.com/catalog/product/view/id/1314482026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08903-1-papss1-primary-antibodies-wb-testing-1.jpgAnti-PAPSS1 Antibody Picoband®Western blot analysis of PAPSS1 using anti-PAPSS1 antibody (A08903-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAPSS1 antigen affinity purified polyclonal antibody (A08903-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAPSS1 at approximately 70 kDa. The expected band size for PAPSS1 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08903-1-papss1-primary-antibodies-if-testing-1.jpgAnti-PAPSS1 Antibody Picoband®IF analysis of PAPSS1 using anti-PAPSS1 antibody (A08903-1) and anti-Alpha Tubulin antibody (M03989-3). PAPSS1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PAPSS1 Antibody (A08903-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1314492026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314502026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314512026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314522026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314532026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314542026-03-16T05:10:03+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314552026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314562026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04056-2-prkd2-primary-antibodies-wb-testing-1.jpgAnti-PRKD2 Antibody Picoband®Western blot analysis of PRKD2 using anti-PRKD2 antibody (A04056-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKD2 antigen affinity purified polyclonal antibody (A04056-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRKD2 at approximately 105 kDa. The expected band size for PRKD2 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04056-2-prkd2-primary-antibodies-if-testing-1.jpgAnti-PRKD2 Antibody Picoband®IF analysis of PRKD2 using anti-PRKD2 antibody (A04056-2). PRKD2 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRKD2 Antibody (A04056-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04056-2-prkd2-primary-antibodies-ip-testing-1.jpgAnti-PRKD2 Antibody Picoband®Immunoprecipitating (IP) PRKD2 in HEL whole cell lysate. Western blot analysis of PRKD2 using anti-PRKD2 antibody (A04056-2); Lane 1: HEL whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-PRKD2 antibody in HEL whole cell lysate; Lane 3: anti-PRKD2 antibody (2μg) + HEL whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRKD2 antigen affinity purified polyclonal antibody (A04056-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PRKD2 at approximately 105 kDa. The expected band size for PRKD2 is at 97 kDa. https://www.bosterbio.com/catalog/product/view/id/1314572026-03-16T05:10:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07861-2-rap1gds1-primary-antibodies-wb-testing-1.jpgAnti-RAP1GDS1 AntibodyWestern blot analysis of RAP1GDS1 using anti-RAP1GDS1 antibody (A07861-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1GDS1 antigen affinity purified polyclonal antibody (A07861-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAP1GDS1 at approximately 66 kDa. The expected band size for RAP1GDS1 is at 66 kDa. https://www.bosterbio.com/catalog/product/view/id/1314582026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314592026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314612026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11737-1-dstyk-primary-antibodies-wb-testing-1.jpgAnti-DSTYK Antibody Picoband®Western blot analysis of DSTYK using anti-DSTYK antibody (A11737-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DSTYK antigen affinity purified polyclonal antibody (A11737-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DSTYK at approximately 105 kDa. The expected band size for DSTYK is at 105 kDa. https://www.bosterbio.com/catalog/product/view/id/1314622026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314632026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314642026-04-02T05:01:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04604-1-eif5b-primary-antibodies-wb-testing-1.jpgAnti-EIF5B Antibody Picoband®Western blot analysis of EIF5B using anti-EIF5B antibody (A04604-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF5B antigen affinity purified polyclonal antibody (A04604-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EIF5B at approximately 200 kDa. The expected band size for EIF5B is at 139 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04604-1-eif5b-primary-antibodies-ihc-testing-1.jpgAnti-EIF5B Antibody Picoband®IHC analysis of EIF5B using anti-EIF5B antibody (A04604-1). EIF5B was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF5B Antibody (A04604-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04604-1-eif5b-primary-antibodies-ihc-testing-2.jpgAnti-EIF5B Antibody Picoband®IHC analysis of EIF5B using anti-EIF5B antibody (A04604-1). EIF5B was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF5B Antibody (A04604-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04604-1-eif5b-primary-antibodies-ihc-testing-3.jpgAnti-EIF5B Antibody Picoband®IHC analysis of EIF5B using anti-EIF5B antibody (A04604-1). EIF5B was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF5B Antibody (A04604-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04604-1-eif5b-primary-antibodies-ihc-testing-4.jpgAnti-EIF5B Antibody Picoband®IHC analysis of EIF5B using anti-EIF5B antibody (A04604-1). EIF5B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF5B Antibody (A04604-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04604-1-eif5b-primary-antibodies-if-testing-1.jpgAnti-EIF5B Antibody Picoband®IF analysis of EIF5B using anti-EIF5B antibody (A04604-1). EIF5B was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EIF5B Antibody (A04604-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04604-1-eif5b-primary-antibodies-ip-testing-1.jpgAnti-EIF5B Antibody Picoband®Immunoprecipitating EIF5B in HepG2 whole cell lysate. Western blot analysis of EIF5B using anti-EIF5B antibody (A04604-1). Lane 1: HepG2 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-EIF5B antibody in HepG2 whole cell lysate, Lane 3: anti-EIF5B antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EIF5B antigen affinity purified polyclonal antibody (A04604-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EIF5B at approximately 200 kDa. The expected band size for EIF5B is at 139 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04604-1-eif5b-primary-antibodies-fcm-testing-1.jpgAnti-EIF5B Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-EIF5B antibody (A04604-1). Overlay histogram showing Jurkat cells stained with A04604-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF5B Antibody (A04604-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1314652026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314662026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314672026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314682026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314692026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314702026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314712026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01887-1-sorl1-primary-antibodies-wb-testing-1.jpgAnti-SORLA/SORL1 Antibody Picoband®Western blot analysis of SORLA/SORL1 using anti-SORLA/SORL1 antibody (A01887-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SORLA/SORL1 antigen affinity purified polyclonal antibody (A01887-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SORLA/SORL1 at approximately 250 kDa. The expected band size for SORLA/SORL1 is at 248 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01887-1-sorl1-primary-antibodies-ihc-testing-1.jpgAnti-SORLA/SORL1 Antibody Picoband®IHC analysis of SORLA/SORL1 using anti-SORLA/SORL1 antibody (A01887-1). SORLA/SORL1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SORLA/SORL1 Antibody (A01887-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01887-1-sorl1-primary-antibodies-ihc-testing-2.jpgAnti-SORLA/SORL1 Antibody Picoband®IHC analysis of SORLA/SORL1 using anti-SORLA/SORL1 antibody (A01887-1). SORLA/SORL1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SORLA/SORL1 Antibody (A01887-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01887-1-sorl1-primary-antibodies-ihc-testing-3.jpgAnti-SORLA/SORL1 Antibody Picoband®IHC analysis of SORLA/SORL1 using anti-SORLA/SORL1 antibody (A01887-1). SORLA/SORL1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SORLA/SORL1 Antibody (A01887-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01887-1-sorl1-primary-antibodies-if-testing-1.jpgAnti-SORLA/SORL1 Antibody Picoband®IF analysis of SORLA/SORL1 using anti-SORLA/SORL1 antibody (A01887-1). SORLA/SORL1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SORLA/SORL1 Antibody (A01887-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01887-1-sorl1-primary-antibodies-fcm-testing-1.jpgAnti-SORLA/SORL1 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-SORLA/SORL1 antibody (A01887-1). Overlay histogram showing U251 cells stained with A01887-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SORLA/SORL1 Antibody (A01887-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1314722026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314732026-03-16T05:10:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06648-1-nexn-primary-antibodies-ihc-testing-1.jpgAnti-Nexilin/NEXN AntibodyIHC analysis of NEXN using anti-NEXN antibody (A06648-1). NEXN was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-NEXN Antibody (A06648-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06648-1-nexn-primary-antibodies-ihc-testing-2.jpgAnti-Nexilin/NEXN AntibodyIHC analysis of NEXN using anti-NEXN antibody (A06648-1). NEXN was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-NEXN Antibody (A06648-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1314742026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314752026-03-13T05:05:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314762026-03-16T05:10:04+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314772026-03-16T05:10:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08697-fxyd3-primary-antibodies-wb-testing-1.jpgAnti-FXYD3 AntibodyWestern blot analysis of FXYD3 using anti-FXYD3 antibody (A08697). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FXYD3 antigen affinity purified polyclonal antibody (A08697) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FXYD3 at approximately 9 kDa. The expected band size for FXYD3 is at 9 kDa. https://www.bosterbio.com/catalog/product/view/id/1314782026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314792026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314802026-03-16T05:10:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05595-1-bckdhb-primary-antibodies-wb-testing-1.jpgAnti-BCKDHB AntibodyWestern blot analysis of BCKDHB using anti-BCKDHB antibody (A05595-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCKDHB antigen affinity purified polyclonal antibody (A05595-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BCKDHB at approximately 37 kDa. The expected band size for BCKDHB is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05595-1-bckdhb-primary-antibodies-ihc-testing-1.jpgAnti-BCKDHB AntibodyIHC analysis of BCKDHB using anti-BCKDHB antibody (A05595-1). BCKDHB was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-BCKDHB Antibody (A05595-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1314812026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314822026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314842026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314852026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314862026-03-17T05:17:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03274-prm1-primary-antibodies-wb-testing-1.jpgAnti-PRM1 Antibody Picoband®Western blot analysis of PRM1 using anti-PRM1 antibody (A03274). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRM1 antigen affinity purified polyclonal antibody (A03274) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRM1 at approximately 16 kDa. The expected band size for PRM1 is at 7 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03274-prm1-primary-antibodies-if-testing-1.jpgAnti-PRM1 Antibody Picoband®IF analysis of PRM1 using anti-PRM1 antibody (A03274) and anti-Alpha Tubulin antibody (M03989-3). PRM1 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRM1 Antibody (A03274) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03274-prm1-primary-antibodies-fcm-testing-1.jpgAnti-PRM1 Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-PRM1 antibody (A03274). Overlay histogram showing Caco-2 cells stained with A03274 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRM1 Antibody (A03274, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1314872026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314882026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314892026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314902026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314912026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314922026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314932026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01971-2-setbp1-primary-antibodies-wb-testing-1.jpgAnti-SETBP1 Antibody Picoband®Western blot analysis of SETBP1 using anti-SETBP1 antibody (A01971-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SETBP1 antigen affinity purified polyclonal antibody (A01971-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SETBP1 at approximately 150 kDa. The expected band size for SETBP1 is at 170 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01971-2-setbp1-primary-antibodies-if-testing-1.jpgAnti-SETBP1 Antibody Picoband®IF analysis of SETBP1 using anti-SETBP1 antibody (A01971-2) and anti-Beta Tubulin antibody (M01857-3). SETBP1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SETBP1 Antibody (A01971-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01971-2-setbp1-primary-antibodies-fcm-testing-1.jpgAnti-SETBP1 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-SETBP1 antibody (A01971-2). Overlay histogram showing SH-SY5Y cells stained with A01971-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SETBP1 Antibody (A01971-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1314942026-03-16T05:10:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06464-1-smarcd3-primary-antibodies-wb-testing-1.jpgAnti-SMARCD3 AntibodyWestern blot analysis of SMARCD3 using anti-SMARCD3 antibody (A06464-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCD3 antigen affinity purified polyclonal antibody (A06464-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMARCD3 at approximately 60 kDa. The expected band size for SMARCD3 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06464-1-smarcd3-primary-antibodies-ihc-testing-1.jpgAnti-SMARCD3 AntibodyIHC analysis of SMARCD3 using anti-SMARCD3 antibody (A06464-1). SMARCD3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-SMARCD3 Antibody (A06464-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06464-1-smarcd3-primary-antibodies-ihc-testing-2.jpgAnti-SMARCD3 AntibodyIHC analysis of SMARCD3 using anti-SMARCD3 antibody (A06464-1). SMARCD3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-SMARCD3 Antibody (A06464-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06464-1-smarcd3-primary-antibodies-ihc-testing-3.jpgAnti-SMARCD3 AntibodyIHC analysis of SMARCD3 using anti-SMARCD3 antibody (A06464-1). SMARCD3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-SMARCD3 Antibody (A06464-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1314952026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314962026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07466-1-taok2-primary-antibodies-wb-testing-1.jpgAnti-TAOK2 Antibody Picoband®Western blot analysis of TAOK2 using anti-TAOK2 antibody (A07466-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAOK2 antigen affinity purified polyclonal antibody (A07466-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAOK2 at approximately 150 kDa. The expected band size for TAOK2 is at 138 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07466-1-taok2-primary-antibodies-ihc-testing-1.jpgAnti-TAOK2 Antibody Picoband®IHC analysis of TAOK2 using anti-TAOK2 antibody (A07466-1). TAOK2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAOK2 Antibody (A07466-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07466-1-taok2-primary-antibodies-ihc-testing-2.jpgAnti-TAOK2 Antibody Picoband®IHC analysis of TAOK2 using anti-TAOK2 antibody (A07466-1). TAOK2 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAOK2 Antibody (A07466-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07466-1-taok2-primary-antibodies-if-testing-1.jpgAnti-TAOK2 Antibody Picoband®IF analysis of TAOK2 using anti-TAOK2 antibody (A07466-1). TAOK2 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TAOK2 Antibody (A07466-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07466-1-taok2-primary-antibodies-fcm-testing-1.jpgAnti-TAOK2 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-TAOK2 antibody (A07466-1). Overlay histogram showing 293T cells stained with A07466-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TAOK2 Antibody (A07466-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1314972026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314982026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1314992026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04701-2-tmod1-primary-antibodies-wb-testing-1.jpgAnti-TMOD1 Antibody Picoband®Western blot analysis of TMOD1 using anti-TMOD1 antibody (A04701-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMOD1 antigen affinity purified polyclonal antibody (A04701-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMOD1 at approximately 41 kDa. The expected band size for TMOD1 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04701-2-tmod1-primary-antibodies-ihc-testing-1.jpgAnti-TMOD1 Antibody Picoband®IHC analysis of TMOD1 using anti-TMOD1 antibody (A04701-2). TMOD1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMOD1 Antibody (A04701-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04701-2-tmod1-primary-antibodies-ihc-testing-2.jpgAnti-TMOD1 Antibody Picoband®IHC analysis of TMOD1 using anti-TMOD1 antibody (A04701-2). TMOD1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMOD1 Antibody (A04701-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04701-2-tmod1-primary-antibodies-ihc-testing-3.jpgAnti-TMOD1 Antibody Picoband®IHC analysis of TMOD1 using anti-TMOD1 antibody (A04701-2). TMOD1 was detected in a paraffin-embedded section of rat erythrocyte tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMOD1 Antibody (A04701-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04701-2-tmod1-primary-antibodies-ihc-testing-4.jpgAnti-TMOD1 Antibody Picoband®IHC analysis of TMOD1 using anti-TMOD1 antibody (A04701-2). TMOD1 was detected in a paraffin-embedded section of mouse erythrocyte tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMOD1 Antibody (A04701-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04701-2-tmod1-primary-antibodies-ihc-testing-5.jpgAnti-TMOD1 Antibody Picoband®IHC analysis of TMOD1 using anti-TMOD1 antibody (A04701-2). TMOD1 was detected in a paraffin-embedded section of human erythrocyte tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMOD1 Antibody (A04701-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04701-2-tmod1-primary-antibodies-ip-testing-1.jpgAnti-TMOD1 Antibody Picoband®Immunoprecipitating TMOD1 in Hela whole cell lysate. Western blot analysis of TMOD1 using anti-TMOD1 antibody (A04701-2). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TMOD1 antibody in Hela whole cell lysate, Lane 3: anti-TMOD1 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TMOD1 antigen affinity purified polyclonal antibody (A04701-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TMOD1 at approximately 41 kDa. The expected band size for TMOD1 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04701-2-tmod1-primary-antibodies-fcm-testing-1.jpgAnti-TMOD1 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-TMOD1 antibody (A04701-2). Overlay histogram showing MCF-7 cells stained with A04701-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMOD1 Antibody (A04701-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315002026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315012026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315022026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315032026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315042026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315052026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315062026-03-16T05:10:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06874-2-arpc3-primary-antibodies-wb-testing-1.jpgAnti-ARPC3 AntibodyWestern blot analysis of ARPC3 using anti-ARPC3 antibody (A06874-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARPC3 antigen affinity purified polyclonal antibody (A06874-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ARPC3 at approximately 18 kDa. The expected band size for ARPC3 is at 21 kDa. https://www.bosterbio.com/catalog/product/view/id/1315072026-03-16T05:10:05+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315082026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315092026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315102026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315112026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315122026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315132026-03-16T05:10:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04812-1-esco2-primary-antibodies-wb-testing-1.jpgAnti-ESCO2 AntibodyWestern blot analysis of ESCO2 using anti-ESCO2 antibody (A04812-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESCO2 antigen affinity purified polyclonal antibody (A04812-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ESCO2 at approximately 70 kDa. The expected band size for ESCO2 is at 68 kDa. https://www.bosterbio.com/catalog/product/view/id/1315142026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315152026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315162026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315172026-04-02T05:01:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08458-1-emg1-primary-antibodies-wb-testing-1.jpgAnti-EMG1 Antibody Picoband®Western blot analysis of EMG1 using anti-EMG1 antibody (A08458-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EMG1 antigen affinity purified polyclonal antibody (A08458-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EMG1 at approximately 27 kDa. The expected band size for EMG1 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08458-1-emg1-primary-antibodies-ihc-testing-1.jpgAnti-EMG1 Antibody Picoband®IHC analysis of EMG1 using anti-EMG1 antibody (A08458-1). EMG1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EMG1 Antibody (A08458-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08458-1-emg1-primary-antibodies-if-testing-1.jpgAnti-EMG1 Antibody Picoband®IF analysis of EMG1 using anti-EMG1 antibody (A08458-1) and anti-Alpha Tubulin antibody (M03989-3). EMG1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EMG1 Antibody (A08458-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1315182026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315192026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315202026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315212026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315222026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315232026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315242026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315252026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315262026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315272026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315282026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315292026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315302026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08517-1-nme3-primary-antibodies-wb-testing-1.jpgAnti-NME3 Antibody Picoband®Western blot analysis of NME3 using anti-NME3 antibody (A08517-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NME3 antigen affinity purified polyclonal antibody (A08517-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NME3 at approximately 18 kDa. The expected band size for NME3 is at 19 kDa. https://www.bosterbio.com/catalog/product/view/id/1315312026-03-16T05:10:06+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315322026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315332026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315342026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315352026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315362026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315372026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315382026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315392026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315402026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315412026-03-16T05:10:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13430-1-spcs2-primary-antibodies-wb-testing-1.jpgAnti-SPCS2 AntibodyWestern blot analysis of SPCS2 using anti-SPCS2 antibody (A13430-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse small intestine tissue lysates, Lane 7: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPCS2 antigen affinity purified polyclonal antibody (A13430-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPCS2 at approximately 20 kDa. The expected band size for SPCS2 is at 25 kDa. https://www.bosterbio.com/catalog/product/view/id/1315422026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05154-1-pkp3-primary-antibodies-wb-testing-1.jpgAnti-Plakophilin 3/PKP3 Antibody Picoband®Western blot analysis of PKP3 using anti-PKP3 antibody (A05154-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human CACO-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKP3 antigen affinity purified polyclonal antibody (Catalog # A05154-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PKP3 at approximately 87 kDa. The expected band size for PKP3 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05154-1-pkp3-primary-antibodies-ihc-testing-1.jpgAnti-Plakophilin 3/PKP3 Antibody Picoband®IHC analysis of PKP3 using anti-PKP3 antibody (A05154-1). PKP3 was detected in a paraffin-embedded section of human invasive urothelial carcinoma with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PKP3 Antibody (A05154-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05154-1-pkp3-primary-antibodies-if-testing-1.jpgAnti-Plakophilin 3/PKP3 Antibody Picoband®IF analysis of PKP3 using anti-PKP3 antibody (A05154-1). PKP3 was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PKP3 Antibody (A05154-1) overnight at 4°C. Cy Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1315432026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315442026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05457-1-prnd-primary-antibodies-wb-testing-1.jpgAnti-PRND Antibody Picoband®Western blot analysis of PRND using anti-PRND antibody (A05457-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRND antigen affinity purified polyclonal antibody (A05457-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRND at approximately 20 kDa. The expected band size for PRND is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05457-1-prnd-primary-antibodies-ihc-testing-1.jpgAnti-PRND Antibody Picoband®IHC analysis of PRND using anti-PRND antibody (A05457-1). PRND was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRND Antibody (A05457-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05457-1-prnd-primary-antibodies-if-testing-1.jpgAnti-PRND Antibody Picoband®IF analysis of PRND using anti-PRND antibody (A05457-1). PRND was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PRND Antibody (A05457-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05457-1-prnd-primary-antibodies-if-testing-2.jpgAnti-PRND Antibody Picoband®IF analysis of PRND using anti-PRND antibody (A05457-1). PRND was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRND Antibody (A05457-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05457-1-prnd-primary-antibodies-fcm-testing-1.jpgAnti-PRND Antibody Picoband®Flow Cytometry analysis of U2OS cells using anti-PRND antibody (A05457-1). Overlay histogram showing U2OS cells stained with A05457-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRND Antibody (A05457-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315452026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07207-2-trim59-primary-antibodies-wb-testing-1.jpgAnti-TRIM59 Antibody Picoband®Western blot analysis of TRIM59 using anti-TRIM59 antibody (A07207-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM59 antigen affinity purified polyclonal antibody (A07207-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIM59 at approximately 47 kDa. The expected band size for TRIM59 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07207-2-trim59-primary-antibodies-ihc-testing-1.jpgAnti-TRIM59 Antibody Picoband®IHC analysis of TRIM59 using anti-TRIM59 antibody (A07207-2). TRIM59 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM59 Antibody (A07207-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07207-2-trim59-primary-antibodies-ihc-testing-2.jpgAnti-TRIM59 Antibody Picoband®IHC analysis of TRIM59 using anti-TRIM59 antibody (A07207-2). TRIM59 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM59 Antibody (A07207-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07207-2-trim59-primary-antibodies-ihc-testing-3.jpgAnti-TRIM59 Antibody Picoband®IHC analysis of TRIM59 using anti-TRIM59 antibody (A07207-2). TRIM59 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM59 Antibody (A07207-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07207-2-trim59-primary-antibodies-fcm-testing-1.jpgAnti-TRIM59 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-TRIM59 antibody (A07207-2). Overlay histogram showing K562 cells stained with A07207-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM59 Antibody (A07207-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07207-2-trim59-primary-antibodies-fcm-testing-2.jpgAnti-TRIM59 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-TRIM59 antibody (A07207-2). Overlay histogram showing PC-3 cells stained with A07207-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM59 Antibody (A07207-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315462026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315472026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315482026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315492026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315502026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315512026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315522026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315532026-03-16T05:10:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315542026-03-16T05:10:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315552026-03-16T05:10:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315562026-03-16T05:10:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315572026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09157-1-sox13-primary-antibodies-wb-testing-1.jpgAnti-SOX13 Antibody Picoband®Western blot analysis of SOX13 using anti-SOX13 antibody (A09157-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: mouse pancreas tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX13 antigen affinity purified polyclonal antibody (A09157-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SOX13 at approximately 80 kDa. The expected band size for SOX13 is at 69 kDa. https://www.bosterbio.com/catalog/product/view/id/1315582026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10221-sfmbt2-primary-antibodies-wb-testing-1.jpgAnti-SFMBT2 Antibody Picoband®Western blot analysis of SFMBT2 using anti-SFMBT2 antibody (A10221). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFMBT2 antigen affinity purified polyclonal antibody (A10221) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SFMBT2 at approximately 101 kDa. The expected band size for SFMBT2 is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10221-sfmbt2-primary-antibodies-if-testing-1.jpgAnti-SFMBT2 Antibody Picoband®IF analysis of SFMBT2 using anti-SFMBT2 antibody (A10221) and anti-Beta Tubulin antibody (M01857-3). SFMBT2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SFMBT2 Antibody (A10221) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10221-sfmbt2-primary-antibodies-ip-testing-1.jpgAnti-SFMBT2 Antibody Picoband®Immunoprecipitating SFMBT2 in Jurkat whole cell lysate. Western blot analysis of SFMBT2 using anti-SFMBT2 antibody (A10221). Lane 1: Jurkat whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-SFMBT2 antibody in Jurkat whole cell lysate, Lane 3: anti-SFMBT2 antibody (2μg) + Jurkat whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SFMBT2 antigen affinity purified polyclonal antibody (A10221) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SFMBT2 at approximately 101 kDa. The expected band size for SFMBT2 is at 101 kDa. https://www.bosterbio.com/catalog/product/view/id/1315592026-03-16T05:10:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315602026-03-16T05:10:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315612026-03-16T05:10:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315622026-03-16T05:10:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315632026-03-16T05:10:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315642026-03-16T05:10:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315662026-03-16T05:10:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315672026-03-16T05:10:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315682026-03-16T05:10:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315692026-03-16T05:10:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315702026-03-16T05:10:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315712026-03-16T05:10:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315722026-03-16T05:10:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315732026-03-16T05:10:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315742026-03-16T05:10:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315752026-03-16T05:10:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315762026-03-16T05:10:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315772026-03-16T05:10:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315782026-03-16T05:10:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315792026-03-16T05:10:09+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315802026-03-16T05:10:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315812026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1315822026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09205-2-cebpg-primary-antibodies-wb-testing-1.jpgAnti-CEBPG Antibody Picoband®Western blot analysis of CEBPG using anti-CEBPG antibody (A09205-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBPG antigen affinity purified polyclonal antibody (A09205-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CEBPG at approximately 21 kDa. The expected band size for CEBPG is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09205-2-cebpg-primary-antibodies-fcm-testing-1.jpgAnti-CEBPG Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-CEBPG antibody (A09205-2). Overlay histogram showing U251 cells stained with A09205-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEBPG Antibody (A09205-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315832026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02730-1-eps8-primary-antibodies-wb-testing-1.jpgAnti-EPS8 Antibody Picoband®Western blot analysis of EPS8 using anti-EPS8 antibody (A02730-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: mouse C6 whole cell lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPS8 antigen affinity purified polyclonal antibody (A02730-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPS8 at approximately 97 kDa. The expected band size for EPS8 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02730-1-eps8-primary-antibodies-if-testing-1.jpgAnti-EPS8 Antibody Picoband®IF analysis of EPS8 using anti-EPS8 antibody (A02730-1). EPS8 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EPS8 Antibody (A02730-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02730-1-eps8-primary-antibodies-ip-testing-1.jpgAnti-EPS8 Antibody Picoband®Immunoprecipitating EPS8 in A549 whole cell lysate. Western blot analysis of EPS8 using anti-EPS8 antibody (A02730-1). Lane 1: A549 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-EPS8 antibody in A549 whole cell lysate, Lane 3: anti-EPS8 antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EPS8 antigen affinity purified polyclonal antibody (A02730-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EPS8 at approximately 97 kDa. The expected band size for EPS8 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02730-1-eps8-primary-antibodies-fcm-testing-1.jpgAnti-EPS8 Antibody Picoband®Flow Cytometry analysis of A431 cells using anti-EPS8 antibody (A02730-1). Overlay histogram showing A431 cells stained with A02730-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EPS8 Antibody (A02730-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315842026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01471-1-esd-primary-antibodies-wb-testing-1.jpgAnti-ESD Antibody Picoband®Western blot analysis of ESD using anti-ESD antibody (A01471-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESD antigen affinity purified polyclonal antibody (A01471-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ESD at approximately 31 kDa. The expected band size for ESD is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01471-1-esd-primary-antibodies-if-testing-1.jpgAnti-ESD Antibody Picoband®IF analysis of ESD using anti-ESD antibody (A01471-1) and anti-Beta Tubulin antibody (M01857-3). ESD was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ESD Antibody (A01471-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01471-1-esd-primary-antibodies-fcm-testing-1.jpgAnti-ESD Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-ESD antibody (A01471-1). Overlay histogram showing HepG2 cells stained with A01471-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ESD Antibody (A01471-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315852026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02729-2-dtnb-primary-antibodies-wb-testing-1.jpgAnti-DTNB Antibody Picoband®Western blot analysis of DTNB using anti-DTNB antibody (A02729-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DTNB antigen affinity purified polyclonal antibody (A02729-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DTNB at approximately 71 kDa. The expected band size for DTNB is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02729-2-dtnb-primary-antibodies-fcm-testing-1.jpgAnti-DTNB Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-DTNB antibody (A02729-2). Overlay histogram showing Jurkat cells stained with A02729-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DTNB Antibody (A02729-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315862026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06494-1-hes7-primary-antibodies-wb-testing-1.jpgAnti-HES7 Antibody Picoband®Western blot analysis of HES7 using anti-HES7 antibody (A06494-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat small intestine tissue lysates, Lane 5: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HES7 antigen affinity purified polyclonal antibody (A06494-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HES7 at approximately 25 kDa. The expected band size for HES7 is at 25 kDa. https://www.bosterbio.com/catalog/product/view/id/1315872026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05640-2-erc1-primary-antibodies-wb-testing-1.jpgAnti-ERC1 Antibody Picoband®Western blot analysis of ERC1 using anti-ERC1 antibody (A05640-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates Lane 3: rat brain tissue lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse NIH/3T3 tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERC1 antigen affinity purified polyclonal antibody (A05640-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERC1 at approximately 130 kDa. The expected band size for ERC1 is at 128 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05640-2-erc1-primary-antibodies-if-testing-1.jpgAnti-ERC1 Antibody Picoband®IF analysis of ERC1 using anti-ERC1 antibody (A05640-2). ERC1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ERC1 Antibody (A05640-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05640-2-erc1-primary-antibodies-ip-testing-1.jpgAnti-ERC1 Antibody Picoband®Immunoprecipitating ERC1 in Hela whole cell lysate. Western blot analysis of ERC1 using anti-ERC1 antibody (A05640-2). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-ERC1 antibody in Hela whole cell lysate, Lane 3: anti-ERC1 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERC1 antigen affinity purified polyclonal antibody (A05640-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ERC1 at approximately 130 kDa. The expected band size for ERC1 is at 128 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05640-2-erc1-primary-antibodies-fcm-testing-1.jpgAnti-ERC1 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-ERC1 antibody (A05640-2). Overlay histogram showing Jurkat cells stained with A05640-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERC1 Antibody (A05640-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315882026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02268-2-adora2a-primary-antibodies-wb-testing-1.jpgAnti-ADORA2A Antibody Picoband®Western blot analysis of ADORA2A using anti-ADORA2A antibody (A02268-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADORA2A antigen affinity purified polyclonal antibody (A02268-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ADORA2A at approximately 45 kDa. The expected band size for ADORA2A is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02268-2-adora2a-primary-antibodies-fcm-testing-1.jpgAnti-ADORA2A Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-ADORA2A antibody (A02268-2). Overlay histogram showing SH-SY5Y cells stained with A02268-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ADORA2A Antibody (A02268-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315892026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01027-2-elf3-primary-antibodies-wb-testing-1.jpgAnti-ELF3 Antibody Picoband®Western blot analysis of ELF3 using anti-ELF3 antibody (A01027-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELF3 antigen affinity purified polyclonal antibody (A01027-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ELF3 at approximately 50 kDa. The expected band size for ELF3 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01027-2-elf3-primary-antibodies-ip-testing-1.jpgAnti-ELF3 Antibody Picoband®Immunoprecipitating ELF3 in A549 whole cell lysate. Western blot analysis of ELF3 using anti-ELF3 antibody (A01027-2). Lane 1: A549 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-ELF3 antibody in A549 whole cell lysate, Lane 3: anti-ELF3 antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ELF3 antigen affinity purified polyclonal antibody (A01027-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ELF3 at approximately 50 kDa. The expected band size for ELF3 is at 41 kDa. https://www.bosterbio.com/catalog/product/view/id/1315902026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05030-1-fmod-primary-antibodies-wb-testing-1.jpgAnti-Fibromodulin/FMOD Antibody Picoband®Western blot analysis of FMOD using anti-FMOD antibody (A05030-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMOD antigen affinity purified polyclonal antibody (A05030-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FMOD at approximately 60-70 kDa. The expected band size for FMOD is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05030-1-fmod-primary-antibodies-fcm-testing-1.jpgAnti-Fibromodulin/FMOD Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-FMOD antibody (A05030-1). Overlay histogram showing Caco-2 cells stained with A05030-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-FMOD Antibody (A05030-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315912026-03-17T05:17:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11471-2-emc1-primary-antibodies-wb-testing-1.jpgAnti-KIAA0090/EMC1 Antibody Picoband®Western blot analysis of EMC1 using anti-EMC1 antibody (A11471-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EMC1 antigen affinity purified polyclonal antibody (A11471-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EMC1 at approximately 112 kDa. The expected band size for EMC1 is at 112 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11471-2-emc1-primary-antibodies-ihc-testing-1.jpgAnti-KIAA0090/EMC1 Antibody Picoband®IHC analysis of EMC1 using anti-EMC1 antibody (A11471-2). EMC1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EMC1 Antibody (A11471-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11471-2-emc1-primary-antibodies-ihc-testing-2.jpgAnti-KIAA0090/EMC1 Antibody Picoband®IHC analysis of EMC1 using anti-EMC1 antibody (A11471-2). EMC1 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EMC1 Antibody (A11471-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11471-2-emc1-primary-antibodies-if-testing-1.jpgAnti-KIAA0090/EMC1 Antibody Picoband®IF analysis of EMC1 using anti-EMC1 antibody (A11471-2). EMC1 was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EMC1 Antibody (A11471-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1315922026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13075-2-gpc2-primary-antibodies-wb-testing-1.jpgAnti-Glypican 2/GPC2 Antibody Picoband®Western blot analysis of GPC2 using anti-GPC2 antibody (A13075-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPC2 antigen affinity purified polyclonal antibody (A13075-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GPC2 at approximately 70 kDa. The expected band size for GPC2 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13075-2-gpc2-primary-antibodies-fcm-testing-1.jpgAnti-Glypican 2/GPC2 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-GPC2 antibody (A13075-2). Overlay histogram showing SH-SY5Y cells stained with A13075-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GPC2 Antibody (A13075-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315932026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06152-1-nup50-primary-antibodies-wb-testing-1.jpgAnti-NUP50 Antibody Picoband®Western blot analysis of NUP50 using anti-NUP50 antibody (A06152-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP50 antigen affinity purified polyclonal antibody (A06152-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NUP50 at approximately 55 kDa. The expected band size for NUP50 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06152-1-nup50-primary-antibodies-ihc-testing-1.jpgAnti-NUP50 Antibody Picoband®IHC analysis of NUP50 using anti-NUP50 antibody (A06152-1). NUP50 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUP50 Antibody (A06152-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06152-1-nup50-primary-antibodies-if-testing-1.jpgAnti-NUP50 Antibody Picoband®IF analysis of NUP50 using anti-NUP50 antibody (A06152-1). NUP50 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NUP50 Antibody (A06152-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06152-1-nup50-primary-antibodies-if-testing-2.jpgAnti-NUP50 Antibody Picoband®IF analysis of NUP50 using anti-NUP50 antibody (A06152-1) and anti-Beta Tubulin antibody (M01857-3). NUP50 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUP50 Antibody (A06152-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1315942026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06486-4-oaz1-primary-antibodies-wb-testing-1.jpgAnti-OAZ1 Antibody Picoband®Western blot analysis of OAZ1 using anti-OAZ1 antibody (A06486-4). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAZ1 antigen affinity purified polyclonal antibody (A06486-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OAZ1 at approximately 25 kDa. The expected band size for OAZ1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06486-4-oaz1-primary-antibodies-ihc-testing-1.jpgAnti-OAZ1 Antibody Picoband®IHC analysis of OAZ1 using anti-OAZ1 antibody (A06486-4). OAZ1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAZ1 Antibody (A06486-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06486-4-oaz1-primary-antibodies-ihc-testing-2.jpgAnti-OAZ1 Antibody Picoband®IHC analysis of OAZ1 using anti-OAZ1 antibody (A06486-4). OAZ1 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAZ1 Antibody (A06486-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06486-4-oaz1-primary-antibodies-ihc-testing-3.jpgAnti-OAZ1 Antibody Picoband®IHC analysis of OAZ1 using anti-OAZ1 antibody (A06486-4). OAZ1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAZ1 Antibody (A06486-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06486-4-oaz1-primary-antibodies-fcm-testing-1.jpgAnti-OAZ1 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-OAZ1 antibody (A06486-4). Overlay histogram showing K562 cells stained with A06486-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OAZ1 Antibody (A06486-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315952026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03924-2-grk1-primary-antibodies-wb-testing-1.jpgAnti-GRK1 Antibody Picoband®Western blot analysis of GRK1 using anti-GRK1 antibody (A03924-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse eye tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRK1 antigen affinity purified polyclonal antibody (A03924-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRK1 at approximately 64 kDa. The expected band size for GRK1 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03924-2-grk1-primary-antibodies-ihc-testing-1.jpgAnti-GRK1 Antibody Picoband®IHC analysis of GRK1 using anti-GRK1 antibody (A03924-2). GRK1 was detected in a paraffin-embedded section of mouse eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRK1 Antibody (A03924-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03924-2-grk1-primary-antibodies-ihc-testing-2.jpgAnti-GRK1 Antibody Picoband®IHC analysis of GRK1 using anti-GRK1 antibody (A03924-2). GRK1 was detected in a paraffin-embedded section of rat eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRK1 Antibody (A03924-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1315962026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02686-3-hpgd-primary-antibodies-wb-testing-1.jpgAnti-HPGD Antibody Picoband®Western blot analysis of HPGD using anti-HPGD antibody (A02686-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPGD antigen affinity purified polyclonal antibody (A02686-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HPGD at approximately 26 kDa. The expected band size for HPGD is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02686-3-hpgd-primary-antibodies-ihc-testing-1.jpgAnti-HPGD Antibody Picoband®IHC analysis of HPGD using anti-HPGD antibody (A02686-3). HPGD was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HPGD Antibody (A02686-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02686-3-hpgd-primary-antibodies-ihc-testing-2.jpgAnti-HPGD Antibody Picoband®IHC analysis of HPGD using anti-HPGD antibody (A02686-3). HPGD was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HPGD Antibody (A02686-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02686-3-hpgd-primary-antibodies-ihc-testing-3.jpgAnti-HPGD Antibody Picoband®IHC analysis of HPGD using anti-HPGD antibody (A02686-3). HPGD was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HPGD Antibody (A02686-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02686-3-hpgd-primary-antibodies-ihc-testing-4.jpgAnti-HPGD Antibody Picoband®IHC analysis of HPGD using anti-HPGD antibody (A02686-3). HPGD was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HPGD Antibody (A02686-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02686-3-hpgd-primary-antibodies-if-testing-1.jpgAnti-HPGD Antibody Picoband®IF analysis of HPGD using anti-HPGD antibody (A02686-3). HPGD was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-HPGD Antibody (A02686-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02686-3-hpgd-primary-antibodies-if-testing-2.jpgAnti-HPGD Antibody Picoband®IF analysis of HPGD using anti-HPGD antibody (A02686-3) and anti-Beta Tubulin antibody (M01857-3). HPGD was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HPGD Antibody (A02686-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02686-3-hpgd-primary-antibodies-fcm-testing-1.jpgAnti-HPGD Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-HPGD antibody (A02686-3). Overlay histogram showing HEL cells stained with A02686-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HPGD Antibody (A02686-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315972026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10931-odf1-primary-antibodies-wb-testing-1.jpgAnti-ODF1 Antibody Picoband®Western blot analysis of ODF1 using anti-ODF1 antibody (A10931). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF1 antigen affinity purified polyclonal antibody (A10931) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ODF1 at approximately 26 kDa. The expected band size for ODF1 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10931-odf1-primary-antibodies-fcm-testing-1.jpgAnti-ODF1 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-ODF1 antibody (A10931). Overlay histogram showing SH-SY5Y cells stained with A10931 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ODF1 Antibody (A10931, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1315982026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09353-3-opn3-primary-antibodies-wb-testing-1.jpgAnti-OPN3 Antibody Picoband®Western blot analysis of OPN3 using anti-OPN3 antibody (A09353-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPN3 antigen affinity purified polyclonal antibody (A09353-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OPN3 at approximately 45 kDa. The expected band size for OPN3 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09353-3-opn3-primary-antibodies-ihc-testing-1.jpgAnti-OPN3 Antibody Picoband®IHC analysis of OPN3 using anti-OPN3 antibody (A09353-3). OPN3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OPN3 Antibody (A09353-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09353-3-opn3-primary-antibodies-ihc-testing-2.jpgAnti-OPN3 Antibody Picoband®IHC analysis of OPN3 using anti-OPN3 antibody (A09353-3). OPN3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OPN3 Antibody (A09353-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09353-3-opn3-primary-antibodies-ihc-testing-3.jpgAnti-OPN3 Antibody Picoband®IHC analysis of OPN3 using anti-OPN3 antibody (A09353-3). OPN3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OPN3 Antibody (A09353-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09353-3-opn3-primary-antibodies-ihc-testing-4.jpgAnti-OPN3 Antibody Picoband®IHC analysis of OPN3 using anti-OPN3 antibody (A09353-3). OPN3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OPN3 Antibody (A09353-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09353-3-opn3-primary-antibodies-if-testing-1.jpgAnti-OPN3 Antibody Picoband®IF analysis of OPN3 using anti-OPN3 antibody (A09353-3). OPN3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-OPN3 Antibody (A09353-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09353-3-opn3-primary-antibodies-if-testing-2.jpgAnti-OPN3 Antibody Picoband®IF analysis of OPN3 using anti-OPN3 antibody (A09353-3). OPN3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-OPN3 Antibody (A09353-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1315992026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06103-2-gfra2-primary-antibodies-wb-testing-1.jpgAnti-GFRA2 Antibody Picoband®Western blot analysis of GFRA2 using anti-GFRA2 antibody (A06103-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFRA2 antigen affinity purified polyclonal antibody (A06103-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GFRA2 at approximately 52 kDa. The expected band size for GFRA2 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06103-2-gfra2-primary-antibodies-ihc-testing-1.jpgAnti-GFRA2 Antibody Picoband®IHC analysis of GFRA2 using anti-GFRA2 antibody (A06103-2). GFRA2 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFRA2 Antibody (A06103-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06103-2-gfra2-primary-antibodies-ihc-testing-2.jpgAnti-GFRA2 Antibody Picoband®IHC analysis of GFRA2 using anti-GFRA2 antibody (A06103-2). GFRA2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFRA2 Antibody (A06103-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06103-2-gfra2-primary-antibodies-ihc-testing-3.jpgAnti-GFRA2 Antibody Picoband®IHC analysis of GFRA2 using anti-GFRA2 antibody (A06103-2). GFRA2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFRA2 Antibody (A06103-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06103-2-gfra2-primary-antibodies-ihc-testing-4.jpgAnti-GFRA2 Antibody Picoband®IHC analysis of GFRA2 using anti-GFRA2 antibody (A06103-2). GFRA2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFRA2 Antibody (A06103-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06103-2-gfra2-primary-antibodies-if-testing-1.jpgAnti-GFRA2 Antibody Picoband®IF analysis of GFRA2 using anti-GFRA2 antibody (A06103-2). GFRA2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GFRA2 Antibody (A06103-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06103-2-gfra2-primary-antibodies-if-testing-2.jpgAnti-GFRA2 Antibody Picoband®IF analysis of GFRA2 using anti-GFRA2 antibody (A06103-2). GFRA2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GFRA2 Antibody (A06103-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06103-2-gfra2-primary-antibodies-fcm-testing-1.jpgAnti-GFRA2 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-GFRA2 antibody (A06103-2). Overlay histogram showing HepG2 cells stained with A06103-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GFRA2 Antibody (A06103-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316002026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05621-nid1-primary-antibodies-wb-testing-1.jpgAnti-Entactin/NID1 Antibody Picoband®Western blot analysis of NID1 using anti-NID1 antibody (A05621). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NID1 antigen affinity purified polyclonal antibody (A05621) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NID1 at approximately 150 kDa. The expected band size for NID1 is at 136 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05621-nid1-primary-antibodies-ihc-testing-1.jpgAnti-Entactin/NID1 Antibody Picoband®IHC analysis of NID1 using anti-NID1 antibody (A05621). NID1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NID1 Antibody (A05621) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05621-nid1-primary-antibodies-if-testing-1.jpgAnti-Entactin/NID1 Antibody Picoband®IF analysis of NID1 using anti-NID1 antibody (A05621). NID1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NID1 Antibody (A05621) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05621-nid1-primary-antibodies-fcm-testing-1.jpgAnti-Entactin/NID1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-NID1 antibody (A05621). Overlay histogram showing HepG2 cells stained with A05621 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NID1 Antibody (A05621, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316012026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04100-3-bclaf1-primary-antibodies-wb-testing-1_1.jpgAnti-BCLAF1 Antibody Picoband®Western blot analysis of BCLAF1 using anti-BCLAF1 antibody (A04100-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCLAF1 antigen affinity purified polyclonal antibody (A04100-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BCLAF1 at approximately 150 kDa. The expected band size for BCLAF1 is at 106 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04100-3-bclaf1-primary-antibodies-ip-testing-1.jpgAnti-BCLAF1 Antibody Picoband®Immunoprecipitating BCLAF1 in Hela whole cell lysate. Western blot analysis of BCLAF1 using anti-BCLAF1 antibody (A04100-3). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-BCLAF1 antibody in Hela whole cell lysate, Lane 3: anti-BCLAF1 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BCLAF1 antigen affinity purified polyclonal antibody (A04100-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BCLAF1 at approximately 150 kDa. The expected band size for BCLAF1 is at 106 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04100-3-bclaf1-primary-antibodies-fcm-testing-1.jpgAnti-BCLAF1 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-BCLAF1 antibody (A04100-3). Overlay histogram showing HEL cells stained with A04100-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCLAF1 Antibody (A04100-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04100-3-bclaf1-primary-antibodies-if-testing-1.jpgAnti-BCLAF1 Antibody Picoband®IF analysis of BCLAF1 using anti-BCLAF1 antibody (A04100-3) and anti-Beta Tubulin antibody (M01857-3). BCLAF1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BCLAF1 Antibody (A04100-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04100-3-bclaf1-primary-antibodies-ihc-testing-4.jpgAnti-BCLAF1 Antibody Picoband®IHC analysis of BCLAF1 using anti-BCLAF1 antibody (A04100-3). BCLAF1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCLAF1 Antibody (A04100-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04100-3-bclaf1-primary-antibodies-ihc-testing-3.jpgAnti-BCLAF1 Antibody Picoband®IHC analysis of BCLAF1 using anti-BCLAF1 antibody (A04100-3). BCLAF1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCLAF1 Antibody (A04100-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04100-3-bclaf1-primary-antibodies-ihc-testing-2.jpgAnti-BCLAF1 Antibody Picoband®IHC analysis of BCLAF1 using anti-BCLAF1 antibody (A04100-3). BCLAF1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCLAF1 Antibody (A04100-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04100-3-bclaf1-primary-antibodies-ihc-testing-1.jpgAnti-BCLAF1 Antibody Picoband®IHC analysis of BCLAF1 using anti-BCLAF1 antibody (A04100-3). BCLAF1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCLAF1 Antibody (A04100-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04100-3-bclaf1-primary-antibodies-ihc-testing-5.jpgAnti-BCLAF1 Antibody Picoband®IHC analysis of BCLAF1 using anti-BCLAF1 antibody (A04100-3). BCLAF1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCLAF1 Antibody (A04100-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1316022026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01073-3-hck-primary-antibodies-wb-testing-1.jpgAnti-HCK Antibody Picoband®Western blot analysis of HCK using anti-HCK antibody (A01073-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCK antigen affinity purified polyclonal antibody (A01073-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HCK at approximately 60 kDa. The expected band size for HCK is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01073-3-hck-primary-antibodies-fcm-testing-1.jpgAnti-HCK Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-HCK antibody (A01073-3). Overlay histogram showing THP-1 cells stained with A01073-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-HCK Antibody (A01073-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316032026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05882-2-impa1-primary-antibodies-wb-testing-1.jpgAnti-IMPA1 Antibody Picoband®Western blot analysis of IMPA1 using anti-IMPA1 antibody (A05882-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IMPA1 antigen affinity purified polyclonal antibody (A05882-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IMPA1 at approximately 30 kDa. The expected band size for IMPA1 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05882-2-impa1-primary-antibodies-ip-testing-1.jpgAnti-IMPA1 Antibody Picoband®Immunoprecipitating IMPA1 in Jurkat whole cell lysate. Western blot analysis of IMPA1 using anti-IMPA1 antibody (A05882-2). Lane 1: Jurkat whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-IMPA1 antibody in Jurkat whole cell lysate, Lane 3: anti-IMPA1 antibody (2μg) + Jurkat whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IMPA1 antigen affinity purified polyclonal antibody (A05882-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IMPA1 at approximately 30 kDa. The expected band size for IMPA1 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05882-2-impa1-primary-antibodies-fcm-testing-1.jpgAnti-IMPA1 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-IMPA1 antibody (A05882-2). Overlay histogram showing Jurkat cells stained with A05882-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IMPA1 Antibody (A05882-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316042026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04213-1-neu3-primary-antibodies-wb-testing-1.jpgAnti-NEU3 Antibody Picoband®Western blot analysis of NEU3 using anti-NEU3 antibody (A04213-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEU3 antigen affinity purified polyclonal antibody (A04213-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NEU3 at approximately 52 kDa. The expected band size for NEU3 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04213-1-neu3-primary-antibodies-fcm-testing-1.jpgAnti-NEU3 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-NEU3 antibody (A04213-1). Overlay histogram showing 293T cells stained with A04213-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NEU3 Antibody (A04213-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316052026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03221-1-nmnat1-primary-antibodies-wb-testing-1.jpgAnti-NMNAT1 Antibody Picoband®Western blot analysis of NMNAT1 using anti-NMNAT1 antibody (A03221-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NMNAT1 antigen affinity purified polyclonal antibody (A03221-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NMNAT1 at approximately 28 kDa. The expected band size for NMNAT1 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03221-1-nmnat1-primary-antibodies-ihc-testing-1.jpgAnti-NMNAT1 Antibody Picoband®IHC analysis of NMNAT1 using anti-NMNAT1 antibody (A03221-1). NMNAT1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NMNAT1 Antibody (A03221-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03221-1-nmnat1-primary-antibodies-if-testing-1.jpgAnti-NMNAT1 Antibody Picoband®IF analysis of NMNAT1 using anti-NMNAT1 antibody (A03221-1). NMNAT1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NMNAT1 Antibody (A03221-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03221-1-nmnat1-primary-antibodies-if-testing-2.jpgAnti-NMNAT1 Antibody Picoband®IF analysis of NMNAT1 using anti-NMNAT1 antibody (A03221-1). NMNAT1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-NMNAT1 Antibody (A03221-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03221-1-nmnat1-primary-antibodies-if-testing-3.jpgAnti-NMNAT1 Antibody Picoband®IF analysis of NMNAT1 using anti-NMNAT1 antibody (A03221-1) and anti-Beta Tubulin antibody (M01857-3). NMNAT1 was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NMNAT1 Antibody (A03221-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03221-1-nmnat1-primary-antibodies-fcm-testing-1.jpgAnti-NMNAT1 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-NMNAT1 antibody (A03221-1). Overlay histogram showing 293T cells stained with A03221-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NMNAT1 Antibody (A03221-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316062026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04562-1-rbck1-primary-antibodies-wb-testing-1.jpgAnti-RBCK1 Antibody Picoband®Western blot analysis of RBCK1 using anti-RBCK1 antibody (A04562-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse RenCa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBCK1 antigen affinity purified polyclonal antibody (A04562-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBCK1 at approximately 58 kDa. The expected band size for RBCK1 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04562-1-rbck1-primary-antibodies-if-testing-1.jpgAnti-RBCK1 Antibody Picoband®IF analysis of RBCK1 using anti-RBCK1 antibody (A04562-1). RBCK1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RBCK1 Antibody (A04562-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04562-1-rbck1-primary-antibodies-fcm-testing-1.jpgAnti-RBCK1 Antibody Picoband®Flow Cytometry analysis of A431 cells using anti-RBCK1 antibody (A04562-1). Overlay histogram showing A431 cells stained with A04562-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBCK1 Antibody (A04562-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316072026-03-17T05:17:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06415-2-prp4k-primary-antibodies-wb-testing-1.jpgAnti-PRP4K Antibody Picoband®Western blot analysis of PRP4K using anti-PRP4K antibody (A06415-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRP4K antigen affinity purified polyclonal antibody (A06415-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRP4K at approximately 140 kDa. The expected band size for PRP4K is at 117 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06415-2-prp4k-primary-antibodies-if-testing-1.jpgAnti-PRP4K Antibody Picoband®IF analysis of PRP4K using anti-PRP4K antibody (A06415-2) and anti-Beta Tubulin antibody (M01857-3). PRP4K was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRP4K Antibody (A06415-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06415-2-prp4k-primary-antibodies-fcm-testing-1.jpgAnti-PRP4K Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-PRP4K antibody (A06415-2). Overlay histogram showing K562 cells stained with A06415-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRP4K Antibody (A06415-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316082026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03198-rai1-primary-antibodies-wb-testing-1.jpgAnti-RAI1 Antibody Picoband®Western blot analysis of RAI1 using anti-RAI1 antibody (A03198). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAI1 antigen affinity purified polyclonal antibody (A03198) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAI1 at approximately 280 kDa. The expected band size for RAI1 is at 203 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03198-rai1-primary-antibodies-if-testing-1.jpgAnti-RAI1 Antibody Picoband®IF analysis of RAI1 using anti-RAI1 antibody (A03198) and anti-Beta Tubulin antibody (M01857-3). RAI1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAI1 Antibody (A03198) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03198-rai1-primary-antibodies-fcm-testing-1.jpgAnti-RAI1 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-RAI1 antibody (A03198). Overlay histogram showing MCF-7 cells stained with A03198 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAI1 Antibody (A03198, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316092026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05853-1-sall2-primary-antibodies-wb-testing-1.jpgAnti-SALL2 Antibody Picoband®Western blot analysis of SALL2 using anti-SALL2 antibody (A05853-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SALL2 antigen affinity purified polyclonal antibody (A05853-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SALL2 at approximately 150 kDa. The expected band size for SALL2 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05853-1-sall2-primary-antibodies-ihc-testing-1.jpgAnti-SALL2 Antibody Picoband®IHC analysis of SALL2 using anti-SALL2 antibody (A05853-1). SALL2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SALL2 Antibody (A05853-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05853-1-sall2-primary-antibodies-ihc-testing-2.jpgAnti-SALL2 Antibody Picoband®IHC analysis of SALL2 using anti-SALL2 antibody (A05853-1). SALL2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SALL2 Antibody (A05853-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05853-1-sall2-primary-antibodies-ihc-testing-3.jpgAnti-SALL2 Antibody Picoband®IHC analysis of SALL2 using anti-SALL2 antibody (A05853-1). SALL2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SALL2 Antibody (A05853-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05853-1-sall2-primary-antibodies-ihc-testing-4.jpgAnti-SALL2 Antibody Picoband®IHC analysis of SALL2 using anti-SALL2 antibody (A05853-1). SALL2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SALL2 Antibody (A05853-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05853-1-sall2-primary-antibodies-if-testing-1.jpgAnti-SALL2 Antibody Picoband®IF analysis of SALL2 using anti-SALL2 antibody (A05853-1). SALL2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SALL2 Antibody (A05853-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05853-1-sall2-primary-antibodies-if-testing-2.jpgAnti-SALL2 Antibody Picoband®IF analysis of SALL2 using anti-SALL2 antibody (A05853-1). SALL2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SALL2 Antibody (A05853-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05853-1-sall2-primary-antibodies-if-testing-3.jpgAnti-SALL2 Antibody Picoband®IF analysis of SALL2 using anti-SALL2 antibody (A05853-1) and anti-Beta Tubulin antibody (M01857-3). SALL2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SALL2 Antibody (A05853-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05853-1-sall2-primary-antibodies-fcm-testing-1.jpgAnti-SALL2 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-SALL2 antibody (A05853-1). Overlay histogram showing SH-SY5Y cells stained with A05853-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SALL2 Antibody (A05853-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05853-1-sall2-primary-antibodies-ihc-testing-5.jpgAnti-SALL2 Antibody Picoband®IHC analysis of SALL2 using anti-SALL2 antibody (A05853-1). SALL2 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SALL2 Antibody (A05853-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1316102026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05939-trip11-primary-antibodies-wb-testing-1.jpgAnti-GMAP-210/TRIP11 Antibody Picoband®Western blot analysis of TRIP11 using anti-TRIP11 antibody (A05939). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIP11 antigen affinity purified polyclonal antibody (A05939) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIP11 at approximately 250 kDa. The expected band size for TRIP11 is at 228 kDa. https://www.bosterbio.com/catalog/product/view/id/1316112026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-wb-testing-1.jpgAnti-PSMD1 Antibody Picoband®Western blot analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD1 antigen affinity purified polyclonal antibody (A06293-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMD1 at approximately 106 kDa. The expected band size for PSMD1 is at 106 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-ihc-testing-1.jpgAnti-PSMD1 Antibody Picoband®IHC analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-ihc-testing-2.jpgAnti-PSMD1 Antibody Picoband®IHC analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-ihc-testing-3.jpgAnti-PSMD1 Antibody Picoband®IHC analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-ihc-testing-4.jpgAnti-PSMD1 Antibody Picoband®IHC analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-ihc-testing-5.jpgAnti-PSMD1 Antibody Picoband®IHC analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-ihc-testing-6.jpgAnti-PSMD1 Antibody Picoband®IHC analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-ihc-testing-7.jpgAnti-PSMD1 Antibody Picoband®IHC analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-if-testing-1.jpgAnti-PSMD1 Antibody Picoband®IF analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-if-testing-2.jpgAnti-PSMD1 Antibody Picoband®IF analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-if-testing-3.jpgAnti-PSMD1 Antibody Picoband®IF analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-if-testing-4.jpgAnti-PSMD1 Antibody Picoband®IF analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-if-testing-5.jpgAnti-PSMD1 Antibody Picoband®IF analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-if-testing-6.jpgAnti-PSMD1 Antibody Picoband®IF analysis of PSMD1 using anti-PSMD1 antibody (A06293-1). PSMD1 was detected in a paraffin-embedded section of human esophageal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMD1 Antibody (A06293-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-if-testing-7.jpgAnti-PSMD1 Antibody Picoband®IF analysis of PSMD1 using anti-PSMD1 antibody (A06293-1) and anti-Beta Tubulin antibody (M01857-3). PSMD1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PSMD1 Antibody (A06293-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06293-1-psmd1-primary-antibodies-fcm-testing-1.jpgAnti-PSMD1 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-PSMD1 antibody (A06293-1). Overlay histogram showing A549 cells stained with A06293-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD1 Antibody (A06293-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316122026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-wb-testing-1.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®Western blot analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCAF11 antigen affinity purified polyclonal antibody (A10138-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SCAF11 at approximately 240 kDa. The expected band size for SCAF11 is at 165 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ihc-testing-1.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IHC analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). SCAF11 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCAF11 Antibody (A10138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ihc-testing-2.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IHC analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). SCAF11 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCAF11 Antibody (A10138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ihc-testing-3.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IHC analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). SCAF11 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCAF11 Antibody (A10138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ihc-testing-4.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IHC analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). SCAF11 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCAF11 Antibody (A10138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ihc-testing-5.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IHC analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). SCAF11 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCAF11 Antibody (A10138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ihc-testing-6.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IHC analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). SCAF11 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCAF11 Antibody (A10138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ihc-testing-7.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IHC analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). SCAF11 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCAF11 Antibody (A10138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ihc-testing-8.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IHC analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). SCAF11 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCAF11 Antibody (A10138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ihc-testing-9.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IHC analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). SCAF11 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCAF11 Antibody (A10138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ihc-testing-10.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IHC analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). SCAF11 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCAF11 Antibody (A10138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ihc-testing-11.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IHC analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). SCAF11 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCAF11 Antibody (A10138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ihc-testing-12.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IHC analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). SCAF11 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCAF11 Antibody (A10138-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-if-testing-1.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®IF analysis of SCAF11 using anti-SCAF11 antibody (A10138-1) and anti-Beta Tubulin antibody (M01857-3). SCAF11 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SCAF11 Antibody (A10138-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-ip-testing-1.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®Immunoprecipitating SCAF11 in Hela whole cell lysate. Western blot analysis of SCAF11 using anti-SCAF11 antibody (A10138-1). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-SCAF11 antibody in Hela whole cell lysate, Lane 3: anti-SCAF11 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SCAF11 antigen affinity purified polyclonal antibody (A10138-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SCAF11 at approximately 240 kDa. The expected band size for SCAF11 is at 165 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10138-1-scaf11-primary-antibodies-fcm-testing-1.jpgAnti-SFRS2IP/SCAF11 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-SCAF11 antibody (A10138-1). Overlay histogram showing MCF-7 cells stained with A10138-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCAF11 Antibody (A10138-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316132026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03387-1-npat-primary-antibodies-wb-testing-1.jpgAnti-NPAT Antibody Picoband®Western blot analysis of NPAT using anti-NPAT antibody (A03387-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPAT antigen affinity purified polyclonal antibody (A03387-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NPAT at approximately 230-250 kDa. The expected band size for NPAT is at 154 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03387-1-npat-primary-antibodies-ihc-testing-1.jpgAnti-NPAT Antibody Picoband®IHC analysis of NPAT using anti-NPAT antibody (A03387-1). NPAT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPAT Antibody (A03387-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03387-1-npat-primary-antibodies-ihc-testing-2.jpgAnti-NPAT Antibody Picoband®IHC analysis of NPAT using anti-NPAT antibody (A03387-1). NPAT was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPAT Antibody (A03387-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03387-1-npat-primary-antibodies-if-testing-1.jpgAnti-NPAT Antibody Picoband®IF analysis of NPAT using anti-NPAT antibody (A03387-1) and anti-Beta Tubulin antibody (M01857-3). NPAT was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NPAT Antibody (A03387-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03387-1-npat-primary-antibodies-fcm-testing-1.jpgAnti-NPAT Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-NPAT antibody (A03387-1). Overlay histogram showing Caco-2 cells stained with A03387-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NPAT Antibody (A03387-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316142026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05629-2-nup160-primary-antibodies-wb-testing-1.jpgAnti-NUP160 Antibody Picoband®Western blot analysis of NUP160 using anti-NUP160 antibody (A05629-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP160 antigen affinity purified polyclonal antibody (A05629-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NUP160 at approximately 150 kDa. The expected band size for NUP160 is at 162 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05629-2-nup160-primary-antibodies-if-testing-1.jpgAnti-NUP160 Antibody Picoband®IF analysis of NUP160 using anti-NUP160 antibody (A05629-2) and anti-Beta Tubulin antibody (M01857-3). NUP160 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUP160 Antibody (A05629-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05629-2-nup160-primary-antibodies-fcm-testing-1.jpgAnti-NUP160 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-NUP160 antibody (A05629-2). Overlay histogram showing 293T cells stained with A05629-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUP160 Antibody (A05629-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316152026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10168-setd5-primary-antibodies-wb-testing-1.jpgAnti-SETD5 Antibody Picoband®Western blot analysis of SETD5 using anti-SETD5 antibody (A10168). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SETD5 antigen affinity purified polyclonal antibody (A10168) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SETD5 at approximately 160 kDa. The expected band size for SETD5 is at 158 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10168-setd5-primary-antibodies-fcm-testing-1.jpgAnti-SETD5 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-SETD5 antibody (A10168). Overlay histogram showing SH-SY5Y cells stained with A10168 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SETD5 Antibody (A10168, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316162026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03046-3-hbp1-primary-antibodies-wb-testing-1.jpgAnti-HBP1 Antibody Picoband®Western blot analysis of HBP1 using anti-HBP1 antibody (A03046-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBP1 antigen affinity purified polyclonal antibody (A03046-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HBP1 at approximately 70 kDa. The expected band size for HBP1 is at 58 kDa. https://www.bosterbio.com/catalog/product/view/id/1316172026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02233-1-setx-primary-antibodies-wb-testing-1.jpgAnti-SETX Antibody Picoband®Western blot analysis of SETX using anti-SETX antibody (A02233-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SETX antigen affinity purified polyclonal antibody (A02233-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SETX at approximately 303 kDa. The expected band size for SETX is at 303 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02233-1-setx-primary-antibodies-fcm-testing-1.jpgAnti-SETX Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-SETX antibody (A02233-1). Overlay histogram showing 293T cells stained with A02233-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SETX Antibody (A02233-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316182026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12029-2-sfxn4-primary-antibodies-wb-testing-1.jpgAnti-SFXN4 Antibody Picoband®Western blot analysis of SFXN4 using anti-SFXN4 antibody (A12029-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFXN4 antigen affinity purified polyclonal antibody (A12029-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SFXN4 at approximately 38 kDa. The expected band size for SFXN4 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12029-2-sfxn4-primary-antibodies-ihc-testing-1.jpgAnti-SFXN4 Antibody Picoband®IHC analysis of SFXN4 using anti-SFXN4 antibody (A12029-2). SFXN4 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN4 Antibody (A12029-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12029-2-sfxn4-primary-antibodies-ihc-testing-2.jpgAnti-SFXN4 Antibody Picoband®IHC analysis of SFXN4 using anti-SFXN4 antibody (A12029-2). SFXN4 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN4 Antibody (A12029-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12029-2-sfxn4-primary-antibodies-ihc-testing-3.jpgAnti-SFXN4 Antibody Picoband®IHC analysis of SFXN4 using anti-SFXN4 antibody (A12029-2). SFXN4 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN4 Antibody (A12029-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12029-2-sfxn4-primary-antibodies-ihc-testing-4.jpgAnti-SFXN4 Antibody Picoband®IHC analysis of SFXN4 using anti-SFXN4 antibody (A12029-2). SFXN4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN4 Antibody (A12029-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12029-2-sfxn4-primary-antibodies-if-testing-1.jpgAnti-SFXN4 Antibody Picoband®IF analysis of SFXN4 using anti-SFXN4 antibody (A12029-2). SFXN4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SFXN4 Antibody (A12029-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12029-2-sfxn4-primary-antibodies-if-testing-2.jpgAnti-SFXN4 Antibody Picoband®IF analysis of SFXN4 using anti-SFXN4 antibody (A12029-2) and anti-Beta Tubulin antibody (M01857-3). SFXN4 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SFXN4 Antibody (A12029-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12029-2-sfxn4-primary-antibodies-fcm-testing-1.jpgAnti-SFXN4 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-SFXN4 antibody (A12029-2). Overlay histogram showing A549 cells stained with A12029-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFXN4 Antibody (A12029-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316192026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05690-2-shank1-primary-antibodies-wb-testing-1.jpgAnti-SHANK1 Antibody Picoband®Western blot analysis of SHANK1 using anti-SHANK1 antibody (A05690-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHANK1 antigen affinity purified polyclonal antibody (A05690-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SHANK1 at approximately 220-250 kDa. The expected band size for SHANK1 is at 225 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05690-2-shank1-primary-antibodies-if-testing-1.jpgAnti-SHANK1 Antibody Picoband®IF analysis of SHANK1 using anti-SHANK1 antibody (A05690-2). SHANK1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SHANK1 Antibody (A05690-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05690-2-shank1-primary-antibodies-fcm-testing-1.jpgAnti-SHANK1 Antibody Picoband®Flow Cytometry analysis of U2OS cells using anti-SHANK1 antibody (A05690-2). Overlay histogram showing U2OS cells stained with A05690-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHANK1 Antibody (A05690-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316202026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04515-1-sik2-primary-antibodies-wb-testing-1.jpgAnti-SIK2 Antibody Picoband®Western blot analysis of SIK2 using anti-SIK2 antibody (A04515-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse 3T3-L1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIK2 antigen affinity purified polyclonal antibody (A04515-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SIK2 at approximately 120 kDa. The expected band size for SIK2 is at 104 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04515-1-sik2-primary-antibodies-ihc-testing-1.jpgAnti-SIK2 Antibody Picoband®IHC analysis of SIK2 using anti-SIK2 antibody (A04515-1). SIK2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIK2 Antibody (A04515-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04515-1-sik2-primary-antibodies-ihc-testing-2.jpgAnti-SIK2 Antibody Picoband®IHC analysis of SIK2 using anti-SIK2 antibody (A04515-1). SIK2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIK2 Antibody (A04515-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04515-1-sik2-primary-antibodies-if-testing-1.jpgAnti-SIK2 Antibody Picoband®IF analysis of SIK2 using anti-SIK2 antibody (A04515-1). SIK2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SIK2 Antibody (A04515-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04515-1-sik2-primary-antibodies-if-testing-2.jpgAnti-SIK2 Antibody Picoband®IF analysis of SIK2 using anti-SIK2 antibody (A04515-1) and anti-Beta Tubulin antibody (M01857-3). SIK2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SIK2 Antibody (A04515-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04515-1-sik2-primary-antibodies-fcm-testing-1.jpgAnti-SIK2 Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-SIK2 antibody (A04515-1). Overlay histogram showing Caco-2 cells stained with A04515-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIK2 Antibody (A04515-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316212026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06424-2-sin3b-primary-antibodies-wb-testing-1.jpgAnti-SIN3B Antibody Picoband®Western blot analysis of SIN3B using anti-SIN3B antibody (A06424-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIN3B antigen affinity purified polyclonal antibody (A06424-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SIN3B at approximately 150 kDa. The expected band size for SIN3B is at 133 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06424-2-sin3b-primary-antibodies-ihc-testing-1.jpgAnti-SIN3B Antibody Picoband®IHC analysis of SIN3B using anti-SIN3B antibody (A06424-2). SIN3B was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIN3B Antibody (A06424-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06424-2-sin3b-primary-antibodies-ihc-testing-2.jpgAnti-SIN3B Antibody Picoband®IHC analysis of SIN3B using anti-SIN3B antibody (A06424-2). SIN3B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIN3B Antibody (A06424-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06424-2-sin3b-primary-antibodies-ihc-testing-3.jpgAnti-SIN3B Antibody Picoband®IHC analysis of SIN3B using anti-SIN3B antibody (A06424-2). SIN3B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIN3B Antibody (A06424-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06424-2-sin3b-primary-antibodies-ihc-testing-4.jpgAnti-SIN3B Antibody Picoband®IHC analysis of SIN3B using anti-SIN3B antibody (A06424-2). SIN3B was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIN3B Antibody (A06424-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06424-2-sin3b-primary-antibodies-if-testing-1.jpgAnti-SIN3B Antibody Picoband®IF analysis of SIN3B using anti-SIN3B antibody (A06424-2). SIN3B was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SIN3B Antibody (A06424-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06424-2-sin3b-primary-antibodies-if-testing-2.jpgAnti-SIN3B Antibody Picoband®IF analysis of SIN3B using anti-SIN3B antibody (A06424-2). SIN3B was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SIN3B Antibody (A06424-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06424-2-sin3b-primary-antibodies-if-testing-3.jpgAnti-SIN3B Antibody Picoband®IF analysis of SIN3B using anti-SIN3B antibody (A06424-2) and anti-Beta Tubulin antibody (M01857-3). SIN3B was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SIN3B Antibody (A06424-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06424-2-sin3b-primary-antibodies-fcm-testing-1.jpgAnti-SIN3B Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-SIN3B antibody (A06424-2). Overlay histogram showing Caco-2 cells stained with A06424-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIN3B Antibody (A06424-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316222026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05549-2-sipa1-primary-antibodies-wb-testing-1.jpgAnti-SIPA1 Antibody Picoband®Western blot analysis of SIPA1 using anti-SIPA1 antibody (A05549-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIPA1 antigen affinity purified polyclonal antibody (A05549-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SIPA1 at approximately 130 kDa. The expected band size for SIPA1 is at 112 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05549-2-sipa1-primary-antibodies-if-testing-1.jpgAnti-SIPA1 Antibody Picoband®IF analysis of SIPA1 using anti-SIPA1 antibody (A05549-2) and anti-Beta Tubulin antibody (M01857-3). SIPA1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SIPA1 Antibody (A05549-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05549-2-sipa1-primary-antibodies-fcm-testing-1.jpgAnti-SIPA1 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-SIPA1 antibody (A05549-2). Overlay histogram showing 293T cells stained with A05549-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIPA1 Antibody (A05549-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316232026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03782-2-shank2-primary-antibodies-wb-testing-1.jpgAnti-SHANK2 Antibody Picoband®Western blot analysis of SHANK2 using anti-SHANK2 antibody (A03782-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHANK2 antigen affinity purified polyclonal antibody (A03782-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SHANK2 at approximately 200 kDa. The expected band size for SHANK2 is at 159 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03782-2-shank2-primary-antibodies-fcm-testing-1.jpgAnti-SHANK2 Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-SHANK2 antibody (A03782-2). Overlay histogram showing Caco-2 cells stained with A03782-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHANK2 Antibody (A03782-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316242026-03-17T05:17:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08238-1-shd-primary-antibodies-wb-testing-1.jpgAnti-SHD Antibody Picoband®Western blot analysis of SHD using anti-SHD antibody (A08238-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human SK-N-SH whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHD antigen affinity purified polyclonal antibody (A08238-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SHD at approximately 40-50 kDa. The expected band size for SHD is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08238-1-shd-primary-antibodies-ihc-testing-1.jpgAnti-SHD Antibody Picoband®IHC analysis of SHD using anti-SHD antibody (A08238-1). SHD was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHD Antibody (A08238-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08238-1-shd-primary-antibodies-ihc-testing-2.jpgAnti-SHD Antibody Picoband®IHC analysis of SHD using anti-SHD antibody (A08238-1). SHD was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHD Antibody (A08238-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08238-1-shd-primary-antibodies-ihc-testing-3.jpgAnti-SHD Antibody Picoband®IHC analysis of SHD using anti-SHD antibody (A08238-1). SHD was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHD Antibody (A08238-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08238-1-shd-primary-antibodies-ihc-testing-4.jpgAnti-SHD Antibody Picoband®IHC analysis of SHD using anti-SHD antibody (A08238-1). SHD was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHD Antibody (A08238-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08238-1-shd-primary-antibodies-ihc-testing-5.jpgAnti-SHD Antibody Picoband®IHC analysis of SHD using anti-SHD antibody (A08238-1). SHD was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHD Antibody (A08238-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08238-1-shd-primary-antibodies-ihc-testing-6.jpgAnti-SHD Antibody Picoband®IHC analysis of SHD using anti-SHD antibody (A08238-1). SHD was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHD Antibody (A08238-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08238-1-shd-primary-antibodies-ihc-testing-7.jpgAnti-SHD Antibody Picoband®IHC analysis of SHD using anti-SHD antibody (A08238-1). SHD was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHD Antibody (A08238-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08238-1-shd-primary-antibodies-if-testing-1.jpgAnti-SHD Antibody Picoband®IF analysis of SHD using anti-SHD antibody (A08238-1). SHD was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SHD Antibody (A08238-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08238-1-shd-primary-antibodies-if-testing-2.jpgAnti-SHD Antibody Picoband®IF analysis of SHD using anti-SHD antibody (A08238-1). SHD was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SHD Antibody (A08238-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08238-1-shd-primary-antibodies-fcm-testing-1.jpgAnti-SHD Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-SHD antibody (A08238-1). Overlay histogram showing SH-SY5Y cells stained with A08238-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHD Antibody (A08238-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08238-1-shd-primary-antibodies-ip-testing-1.jpgAnti-SHD Antibody Picoband®Immunoprecipitating (IP) SHD in SH-SY5Y whole cell lysate. Western blot analysis of SHD using anti-SHD antibody (A08238-1); Lane 1: SH-SY5Y whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-SHD antibody in SH-SY5Y whole cell lysate; Lane 3: anti-SHD antibody (2μg) + SH-SY5Y whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SHD antigen affinity purified polyclonal antibody (A08238-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for IDH3B at approximately 40-50 kDa. The expected band size for IDH3B is at 38 kDa. https://www.bosterbio.com/catalog/product/view/id/1316252026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15256-1-she-primary-antibodies-wb-testing-1.jpgAnti-SHE Antibody Picoband®Western blot analysis of SHE using anti-SHE antibody (A15256-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHE antigen affinity purified polyclonal antibody (A15256-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SHE at approximately 54 kDa. The expected band size for SHE is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15256-1-she-primary-antibodies-ihc-testing-1.jpgAnti-SHE Antibody Picoband®IHC analysis of SHE using anti-SHE antibody (A15256-1). SHE was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHE Antibody (A15256-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15256-1-she-primary-antibodies-ihc-testing-2.jpgAnti-SHE Antibody Picoband®IHC analysis of SHE using anti-SHE antibody (A15256-1). SHE was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHE Antibody (A15256-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15256-1-she-primary-antibodies-if-testing-1.jpgAnti-SHE Antibody Picoband®IF analysis of SHE using anti-SHE antibody (A15256-1). SHE was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SHE Antibody (A15256-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15256-1-she-primary-antibodies-if-testing-2.jpgAnti-SHE Antibody Picoband®IF analysis of SHE using anti-SHE antibody (A15256-1). SHE was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SHE Antibody (A15256-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15256-1-she-primary-antibodies-fcm-testing-1.jpgAnti-SHE Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-SHE antibody (A15256-1). Overlay histogram showing THP-1 cells stained with A15256-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHE Antibody (A15256-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316262026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03373-2-sigirr-primary-antibodies-wb-testing-1.jpgAnti-SIGIRR Antibody Picoband®Western blot analysis of SIGIRR using anti-SIGIRR antibody (A03373-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIGIRR antigen affinity purified polyclonal antibody (A03373-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SIGIRR at approximately 80 kDa. The expected band size for SIGIRR is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03373-2-sigirr-primary-antibodies-ihc-testing-1.jpgAnti-SIGIRR Antibody Picoband®IHC analysis of SIGIRR using anti-SIGIRR antibody (A03373-2). SIGIRR was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIGIRR Antibody (A03373-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03373-2-sigirr-primary-antibodies-ihc-testing-2.jpgAnti-SIGIRR Antibody Picoband®IHC analysis of SIGIRR using anti-SIGIRR antibody (A03373-2). SIGIRR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIGIRR Antibody (A03373-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03373-2-sigirr-primary-antibodies-ihc-testing-3.jpgAnti-SIGIRR Antibody Picoband®IHC analysis of SIGIRR using anti-SIGIRR antibody (A03373-2). SIGIRR was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIGIRR Antibody (A03373-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03373-2-sigirr-primary-antibodies-ihc-testing-4.jpgAnti-SIGIRR Antibody Picoband®IHC analysis of SIGIRR using anti-SIGIRR antibody (A03373-2). SIGIRR was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIGIRR Antibody (A03373-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03373-2-sigirr-primary-antibodies-ihc-testing-5.jpgAnti-SIGIRR Antibody Picoband®IHC analysis of SIGIRR using anti-SIGIRR antibody (A03373-2). SIGIRR was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIGIRR Antibody (A03373-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03373-2-sigirr-primary-antibodies-if-testing-1.jpgAnti-SIGIRR Antibody Picoband®IF analysis of SIGIRR using anti-SIGIRR antibody (A03373-2). SIGIRR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SIGIRR Antibody (A03373-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03373-2-sigirr-primary-antibodies-if-testing-2.jpgAnti-SIGIRR Antibody Picoband®IF analysis of SIGIRR using anti-SIGIRR antibody (A03373-2). SIGIRR was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SIGIRR Antibody (A03373-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03373-2-sigirr-primary-antibodies-if-testing-3.jpgAnti-SIGIRR Antibody Picoband®IF analysis of SIGIRR using anti-SIGIRR antibody (A03373-2). SIGIRR was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SIGIRR Antibody (A03373-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03373-2-sigirr-primary-antibodies-fcm-testing-1.jpgAnti-SIGIRR Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-SIGIRR antibody (A03373-2). Overlay histogram showing 293T cells stained with A03373-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SIGIRR Antibody (A03373-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316272026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05580-2-sik3-primary-antibodies-wb-testing-1.jpgAnti-SIK3 Antibody Picoband®Western blot analysis of SIK3 using anti-SIK3 antibody (A05580-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIK3 antigen affinity purified polyclonal antibody (A05580-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SIK3 at approximately 150 kDa. The expected band size for SIK3 is at 145 kDa. https://www.bosterbio.com/catalog/product/view/id/1316282026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14901-2-slc6a16-primary-antibodies-wb-testing-1.jpgAnti-SLC6A16 Antibody Picoband®Western blot analysis of SLC6A16 using anti-SLC6A16 antibody (A14901-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat C6 tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A16 antigen affinity purified polyclonal antibody (A14901-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC6A16 at approximately 82 kDa. The expected band size for SLC6A16 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14901-2-slc6a16-primary-antibodies-ihc-testing-1.jpgAnti-SLC6A16 Antibody Picoband®IHC analysis of SLC6A16 using anti-SLC6A16 antibody (A14901-2). SLC6A16 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC6A16 Antibody (A14901-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14901-2-slc6a16-primary-antibodies-fcm-testing-1.jpgAnti-SLC6A16 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-SLC6A16 antibody (A14901-2). Overlay histogram showing A549 cells stained with A14901-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC6A16 Antibody (A14901-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316292026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05937-1-mtmr3-primary-antibodies-wb-testing-1.jpgAnti-MTMR3 Antibody Picoband®Western blot analysis of MTMR3 using anti-MTMR3 antibody (A05937-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTMR3 antigen affinity purified polyclonal antibody (A05937-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MTMR3 at approximately 150 kDa. The expected band size for MTMR3 is at 134 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05937-1-mtmr3-primary-antibodies-ihc-testing-1.jpgAnti-MTMR3 Antibody Picoband®IHC analysis of MTMR3 using anti-MTMR3 antibody (A05937-1). MTMR3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTMR3 Antibody (A05937-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05937-1-mtmr3-primary-antibodies-ihc-testing-2.jpgAnti-MTMR3 Antibody Picoband®IHC analysis of MTMR3 using anti-MTMR3 antibody (A05937-1). MTMR3 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTMR3 Antibody (A05937-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05937-1-mtmr3-primary-antibodies-ihc-testing-3.jpgAnti-MTMR3 Antibody Picoband®IHC analysis of MTMR3 using anti-MTMR3 antibody (A05937-1). MTMR3 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MTMR3 Antibody (A05937-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05937-1-mtmr3-primary-antibodies-if-testing-1.jpgAnti-MTMR3 Antibody Picoband®IF analysis of MTMR3 using anti-MTMR3 antibody (A05937-1). MTMR3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MTMR3 Antibody (A05937-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05937-1-mtmr3-primary-antibodies-if-testing-2.jpgAnti-MTMR3 Antibody Picoband®IF analysis of MTMR3 using anti-MTMR3 antibody (A05937-1) and anti-Beta Tubulin antibody (M01857-3). MTMR3 was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MTMR3 Antibody (A05937-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05937-1-mtmr3-primary-antibodies-fcm-testing-1.jpgAnti-MTMR3 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-MTMR3 antibody (A05937-1). Overlay histogram showing RT4 cells stained with A05937-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MTMR3 Antibody (A05937-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316302026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08916-2-ntsr2-primary-antibodies-wb-testing-1.jpgAnti-NTSR2 Antibody Picoband®Western blot analysis of NTSR2 using anti-NTSR2 antibody (A08916-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTSR2 antigen affinity purified polyclonal antibody (A08916-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NTSR2 at approximately 45 kDa. The expected band size for NTSR2 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08916-2-ntsr2-primary-antibodies-fcm-testing-1.jpgAnti-NTSR2 Antibody Picoband®Flow Cytometry analysis of Neuro-2a cells using anti-NTSR2 antibody (A08916-2). Overlay histogram showing Neuro-2a cells stained with A08916-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NTSR2 Antibody (A08916-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316312026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01867-1-topbp1-primary-antibodies-wb-testing-1.jpgAnti-TOPBP1 Antibody Picoband®Western blot analysis of TOPBP1 using anti-TOPBP1 antibody (A01867-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOPBP1 antigen affinity purified polyclonal antibody (A01867-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TOPBP1 at approximately 170 kDa. The expected band size for TOPBP1 is at 170 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01867-1-topbp1-primary-antibodies-ihc-testing-1.jpgAnti-TOPBP1 Antibody Picoband®IHC analysis of TOPBP1 using anti-TOPBP1 antibody (A01867-1). TOPBP1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOPBP1 Antibody (A01867-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01867-1-topbp1-primary-antibodies-ihc-testing-2.jpgAnti-TOPBP1 Antibody Picoband®IHC analysis of TOPBP1 using anti-TOPBP1 antibody (A01867-1). TOPBP1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOPBP1 Antibody (A01867-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01867-1-topbp1-primary-antibodies-if-testing-1.jpgAnti-TOPBP1 Antibody Picoband®IF analysis of TOPBP1 using anti-TOPBP1 antibody (A01867-1) and anti-Beta Tubulin antibody (M01857-3). TOPBP1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TOPBP1 Antibody (A01867-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01867-1-topbp1-primary-antibodies-ip-testing-1.jpgAnti-TOPBP1 Antibody Picoband®Immunoprecipitating (IP) TOPBP1 in Hela whole cell lysate. Western blot analysis of TOPBP1 using anti-TOPBP1 antibody (A01867-1); Lane 1: Hela whole cell lysates (30ug); Lane 2: Rabbit control IgG instead of anti-TOPBP1 antibody in Hela whole cell lysate; Lane 3: anti-TOPBP1 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TOPBP1 antigen affinity purified polyclonal antibody (A01867-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for TOPBP1 at approximately 170 kDa. The expected band size for TOPBP1 is at 170 kDa. https://www.bosterbio.com/catalog/product/view/id/1316322026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01304-2-ncor1-primary-antibodies-wb-testing-1.jpgAnti-NCOR1 Antibody Picoband®Western blot analysis of NCOR1 using anti-NCOR1 antibody (A01304-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jyrkat whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOR1 antigen affinity purified polyclonal antibody (A01304-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NCOR1 at approximately 300 kDa. The expected band size for NCOR1 is at 300 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01304-2-ncor1-primary-antibodies-if-testing-1.jpgAnti-NCOR1 Antibody Picoband®IF analysis of NCOR1 using anti-NCOR1 antibody (A01304-2) and anti-Beta Tubulin antibody (M01857-3). NCOR1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NCOR1 Antibody (A01304-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01304-2-ncor1-primary-antibodies-fcm-testing-1.jpgAnti-NCOR1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-NCOR1 antibody (A01304-2). Overlay histogram showing HepG2 cells stained with A01304-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCOR1 Antibody (A01304-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316332026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02233-2-setx-primary-antibodies-wb-testing-1.jpgAnti-SETX Antibody Picoband®Western blot analysis of SETX using anti-SETX antibody (A02233-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat L6 whole cell lysates, Lane 2: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SETX antigen affinity purified polyclonal antibody (A02233-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SETX at approximately 303 kDa. The expected band size for SETX is at 300 kDa. https://www.bosterbio.com/catalog/product/view/id/1316342026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07561-1-ska3-primary-antibodies-wb-testing-1.jpgAnti-C13orf3/SKA3 Antibody Picoband®Western blot analysis of SKA3 using anti-SKA3 antibody (A07561-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKA3 antigen affinity purified polyclonal antibody (A07561-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SKA3 at approximately 51 kDa. The expected band size for SKA3 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07561-1-ska3-primary-antibodies-ihc-testing-1.jpgAnti-C13orf3/SKA3 Antibody Picoband®IHC analysis of SKA3 using anti-SKA3 antibody (A07561-1). SKA3 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SKA3 Antibody (A07561-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1316352026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08467-2-slc9a8-primary-antibodies-wb-testing-1.jpgAnti-NHE8/SLC9A8 Antibody Picoband®Western blot analysis of SLC9A8 using anti-SLC9A8 antibody (A08467-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A8 antigen affinity purified polyclonal antibody (A08467-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC9A8 at approximately 75 kDa. The expected band size for SLC9A8 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08467-2-slc9a8-primary-antibodies-fcm-testing-1.jpgAnti-NHE8/SLC9A8 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-SLC9A8 antibody (A08467-2). Overlay histogram showing 293T cells stained with A08467-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC9A8 Antibody (A08467-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316362026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a19477-slc15a5-primary-antibodies-wb-testing-1.jpgAnti-SLC15A5 Antibody Picoband®Western blot analysis of SLC15A5 using anti-SLC15A5 antibody (A19477). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC15A5 antigen affinity purified polyclonal antibody (A19477) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC15A5 at approximately 70 kDa. The expected band size for SLC15A5 is at 65 kDa. https://www.bosterbio.com/catalog/product/view/id/1316372026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06757-1-slco1c1-primary-antibodies-wb-testing-1.jpgAnti-OATP14/SLCO1C1 Antibody Picoband®Western blot analysis of SLCO1C1 using anti-SLCO1C1 antibody (A06757-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLCO1C1 antigen affinity purified polyclonal antibody (A06757-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLCO1C1 at approximately 80 kDa. The expected band size for SLCO1C1 is at 79 kDa. https://www.bosterbio.com/catalog/product/view/id/1316382026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12129-1-slf2-primary-antibodies-wb-testing-1.jpgAnti-SLF2 Antibody Picoband®Western blot analysis of SLF2 using anti-SLF2 antibody (A12129-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLF2 antigen affinity purified polyclonal antibody (A12129-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLF2 at approximately 150 kDa. The expected band size for SLF2 is at 132 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12129-1-slf2-primary-antibodies-if-testing-1.jpgAnti-SLF2 Antibody Picoband®IF analysis of SLF2 using anti-SLF2 antibody (A12129-1) and anti-Beta Tubulin antibody (M01857-3). SLF2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLF2 Antibody (A12129-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1316392026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03383-2-smarcal1-primary-antibodies-wb-testing-1.jpgAnti-SMARCAL1 Antibody Picoband®Western blot analysis of SMARCAL1 using anti-SMARCAL1 antibody (A03383-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCAL1 antigen affinity purified polyclonal antibody (A03383-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMARCAL1 at approximately 106 kDa. The expected band size for SMARCAL1 is at 106 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03383-2-smarcal1-primary-antibodies-ihc-testing-1.jpgAnti-SMARCAL1 Antibody Picoband®IHC analysis of SMARCAL1 using anti-SMARCAL1 antibody (A03383-2). SMARCAL1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCAL1 Antibody (A03383-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03383-2-smarcal1-primary-antibodies-ihc-testing-2.jpgAnti-SMARCAL1 Antibody Picoband®IHC analysis of SMARCAL1 using anti-SMARCAL1 antibody (A03383-2). SMARCAL1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCAL1 Antibody (A03383-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03383-2-smarcal1-primary-antibodies-ihc-testing-3.jpgAnti-SMARCAL1 Antibody Picoband®IHC analysis of SMARCAL1 using anti-SMARCAL1 antibody (A03383-2). SMARCAL1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMARCAL1 Antibody (A03383-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03383-2-smarcal1-primary-antibodies-fcm-testing-1.jpgAnti-SMARCAL1 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-SMARCAL1 antibody (A03383-2). Overlay histogram showing HEL cells stained with A03383-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMARCAL1 Antibody (A03383-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316402026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04187-3-mybbp1a-primary-antibodies-wb-testing-1.jpgAnti-MYBBP1A Antibody Picoband®Western blot analysis of MYBBP1A using anti-MYBBP1A antibody (A04187-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYBBP1A antigen affinity purified polyclonal antibody (A04187-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYBBP1A at approximately 150 kDa. The expected band size for MYBBP1A is at 149 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04187-3-mybbp1a-primary-antibodies-if-testing-1.jpgAnti-MYBBP1A Antibody Picoband®IF analysis of MYBBP1A using anti-MYBBP1A antibody (A04187-3) and anti-Beta Tubulin antibody (M01857-3). MYBBP1A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MYBBP1A Antibody (A04187-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04187-3-mybbp1a-primary-antibodies-fcm-testing-1.jpgAnti-MYBBP1A Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-MYBBP1A antibody (A04187-3). Overlay histogram showing A549 cells stained with A04187-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYBBP1A Antibody (A04187-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316412026-03-17T05:17:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03856-2-hjurp-primary-antibodies-wb-testing-1.jpgAnti-HJURP Antibody Picoband®Western blot analysis of HJURP using anti-HJURP antibody (A03856-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HJURP antigen affinity purified polyclonal antibody (A03856-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HJURP at approximately 90 kDa. The expected band size for HJURP is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03856-2-hjurp-primary-antibodies-if-testing-1.jpgAnti-HJURP Antibody Picoband®IF analysis of HJURP using anti-HJURP antibody (A03856-2). HJURP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HJURP Antibody (A03856-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03856-2-hjurp-primary-antibodies-fcm-testing-1.jpgAnti-HJURP Antibody Picoband®Flow Cytometry analysis of U2OS cells using anti-HJURP antibody (A03856-2). Overlay histogram showing U2OS cells stained with A03856-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HJURP Antibody (A03856-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lca5-antibody.html2026-03-17T05:17:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316462026-03-10T04:41:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316472026-03-10T04:41:51+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316482026-03-10T04:41:52+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316492026-03-10T04:41:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-apobb-1-antibody.html2026-03-17T05:17:13+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-atg5-antibody.html2026-03-17T05:17:13+00:00daily0.9 https://www.bosterbio.com/products/human-ifn-gamma-picokine-trade-elisa-kit-hsek0373-boster.html2026-03-10T04:41:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0373.jpgHuman IFN Gamma High Sensitivity ELISA Kit PicoKine®Human IFN gamma High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/human-il-8-picokine-trade-elisa-kit-hsek0413-boster.html2026-03-10T04:41:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0413_1.jpgHuman IL-8 High Sensitivity ELISA Kit PicoKine®Human IL-8 High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/mouse-tnf-alpha-picokine-trade-elisa-kit-hsek0527-boster.html2026-03-10T04:41:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0527.jpgMouse TNF Alpha High Sensitivity ELISA Kit PicoKine®Mouse TNF alpha High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/human-il-6-picokine-trade-elisa-kit-hsek0410-boster.html2026-03-10T04:41:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0410.jpgHuman IL-6 High Sensitivity ELISA Kit PicoKine®Human IL-6 High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/mouse-il-1-beta-picokine-trade-elisa-kit-hsek0394-boster.html2026-03-10T04:41:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0394.jpgMouse IL-1 Beta High Sensitivity ELISA Kit PicoKine®Mouse IL-1 beta High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/mouse-il-17-picokine-trade-elisa-kit-hsek0431-boster.html2026-03-10T04:41:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0431.jpgMouse IL-17 High Sensitivity ELISA Kit PicoKine®Mouse IL-17 High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/human-gm-csf-picokine-trade-elisa-kit-hsek0364-boster.html2026-03-10T04:41:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0364.jpgHuman GM-CSF High Sensitivity ELISA Kit PicoKine®Human GM-CSF High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/mouse-il-2-picokine-trade-elisa-kit-hsek0398-boster.html2026-03-10T04:41:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0398.jpgMouse IL-2 High Sensitivity ELISA Kit PicoKine®Mouse IL-2 High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/mouse-ifn-gamma-picokine-trade-elisa-kit-hsek0375-boster.html2026-03-10T04:41:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0375.jpgMouse IFN Gamma High Sensitivity ELISA Kit PicoKine®Mouse IFN gamma High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/mouse-il-6-picokine-trade-elisa-kit-hsek0411-boster.html2026-03-10T04:41:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0411.jpgMouse IL-6 High Sensitivity ELISA Kit PicoKine®Mouse IL-6 High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1316632026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07232-1-slc6a19-primary-antibodies-wb-testing-1.jpgAnti-SLC6A19 Antibody Picoband®Western blot analysis of SLC6A19 using anti-SLC6A19 antibody (A07232-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A19 antigen affinity purified polyclonal antibody (A07232-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC6A19 at approximately 71 kDa. The expected band size for SLC6A19 is at 71 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lrrc10-antibody.html2026-03-17T05:17:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316662026-03-10T04:41:53+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316672026-03-10T04:41:53+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rgs12b-antibody.html2026-03-17T05:17:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316692026-03-16T05:10:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316702026-03-16T05:10:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316712026-03-16T05:10:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316722026-03-16T05:10:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316732026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05756-4-ppp2r1b-primary-antibodies-wb-testing-1.jpgAnti-PPP2R1B Antibody Picoband®Western blot analysis of PPP2R1B using anti-PPP2R1B antibody (A05756-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A2780 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R1B antigen affinity purified polyclonal antibody (A05756-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPP2R1B at approximately 60-74 kDa. The expected band size for PPP2R1B is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05756-4-ppp2r1b-primary-antibodies-ihc-testing-1.jpgAnti-PPP2R1B Antibody Picoband®IHC analysis of PPP2R1B using anti-PPP2R1B antibody (A05756-4). PPP2R1B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2R1B Antibody (A05756-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05756-4-ppp2r1b-primary-antibodies-ihc-testing-2.jpgAnti-PPP2R1B Antibody Picoband®IHC analysis of PPP2R1B using anti-PPP2R1B antibody (A05756-4). PPP2R1B was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2R1B Antibody (A05756-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1316742026-03-16T05:10:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316752026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12382-1-trim47-primary-antibodies-wb-testing-1.jpgAnti-TRIM47 Antibody Picoband®Western blot analysis of TRIM47 using anti-TRIM47 antibody (A12382-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM47 antigen affinity purified polyclonal antibody (A12382-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIM47 at approximately 70 kDa. The expected band size for TRIM47 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12382-1-trim47-primary-antibodies-ihc-testing-1.jpgAnti-TRIM47 Antibody Picoband®IHC analysis of TRIM47 using anti-TRIM47 antibody (A12382-1). TRIM47 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM47 Antibody (A12382-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12382-1-trim47-primary-antibodies-ihc-testing-2.jpgAnti-TRIM47 Antibody Picoband®IHC analysis of TRIM47 using anti-TRIM47 antibody (A12382-1). TRIM47 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM47 Antibody (A12382-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12382-1-trim47-primary-antibodies-ihc-testing-3.jpgAnti-TRIM47 Antibody Picoband®IHC analysis of TRIM47 using anti-TRIM47 antibody (A12382-1). TRIM47 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM47 Antibody (A12382-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12382-1-trim47-primary-antibodies-ihc-testing-4.jpgAnti-TRIM47 Antibody Picoband®IHC analysis of TRIM47 using anti-TRIM47 antibody (A12382-1). TRIM47 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM47 Antibody (A12382-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12382-1-trim47-primary-antibodies-if-testing-1.jpgAnti-TRIM47 Antibody Picoband®IF analysis of TRIM47 using anti-TRIM47 antibody (A12382-1) and anti-Alpha Tubulin antibody (M03989-3). TRIM47 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRIM47 Antibody (A12382-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1316762026-03-16T05:10:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316772026-03-16T05:10:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316782026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316792026-03-16T05:10:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316802026-03-16T05:10:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316812026-03-16T05:10:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316822026-03-16T05:10:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316832026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1316842026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11813-1-slfn14-primary-antibodies-wb-testing-1.jpgAnti-SLFN14 Antibody Picoband®Western blot analysis of SLFN14 using anti-SLFN14 antibody (A11813-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLFN14 antigen affinity purified polyclonal antibody (A11813-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLFN14 at approximately 104 kDa. The expected band size for SLFN14 is at 104 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11813-1-slfn14-primary-antibodies-fcm-testing-1.jpgAnti-SLFN14 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-SLFN14 antibody (A11813-1). Overlay histogram showing K562 cells stained with A11813-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLFN14 Antibody (A11813-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316852026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02388-1-slx4-primary-antibodies-ihc-testing-1.jpgAnti-SLX4 AntibodyIHC analysis of SLX4 using anti-SLX4 antibody (A02388-1). SLX4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLX4 Antibody (A02388-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02388-1-slx4-primary-antibodies-ihc-testing-2.jpgAnti-SLX4 AntibodyIHC analysis of SLX4 using anti-SLX4 antibody (A02388-1). SLX4 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLX4 Antibody (A02388-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02388-1-slx4-primary-antibodies-if-testing-1.jpgAnti-SLX4 AntibodyIF analysis of SLX4 using anti-SLX4 antibody (A02388-1). SLX4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLX4 Antibody (A02388-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02388-1-slx4-primary-antibodies-if-testing-2.jpgAnti-SLX4 AntibodyIF analysis of SLX4 using anti-SLX4 antibody (A02388-1) and anti-Beta Tubulin antibody (M01857-3). SLX4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLX4 Antibody (A02388-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1316862026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10635-2-smg8-primary-antibodies-wb-testing-1.jpgAnti-SMG8 Antibody Picoband®Western blot analysis of SMG8 using anti-SMG8 antibody (A10635-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMG8 antigen affinity purified polyclonal antibody (A10635-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMG8 at approximately 120 kDa. The expected band size for SMG8 is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10635-2-smg8-primary-antibodies-ihc-testing-1.jpgAnti-SMG8 Antibody Picoband®IHC analysis of SMG8 using anti-SMG8 antibody (A10635-2). SMG8 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMG8 Antibody (A10635-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10635-2-smg8-primary-antibodies-ihc-testing-2.jpgAnti-SMG8 Antibody Picoband®IHC analysis of SMG8 using anti-SMG8 antibody (A10635-2). SMG8 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMG8 Antibody (A10635-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10635-2-smg8-primary-antibodies-ihc-testing-3.jpgAnti-SMG8 Antibody Picoband®IHC analysis of SMG8 using anti-SMG8 antibody (A10635-2). SMG8 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SMG8 Antibody (A10635-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10635-2-smg8-primary-antibodies-if-testing-1.jpgAnti-SMG8 Antibody Picoband®IF analysis of SMG8 using anti-SMG8 antibody (A10635-2) and anti-Beta Tubulin antibody (M01857-3). SMG8 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMG8 Antibody (A10635-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10635-2-smg8-primary-antibodies-fcm-testing-1.jpgAnti-SMG8 Antibody Picoband®Flow Cytometry analysis of U2OS cells using anti-SMG8 antibody (A10635-2). Overlay histogram showing U2OS cells stained with A10635-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMG8 Antibody (A10635-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316872026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11617-1-snap47-primary-antibodies-wb-testing-1.jpgAnti-SNAP47 Antibody Picoband®Western blot analysis of SNAP47 using anti-SNAP47 antibody (A11617-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP47 antigen affinity purified polyclonal antibody (A11617-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNAP47 at approximately 47 kDa. The expected band size for SNAP47 is at 53 kDa. https://www.bosterbio.com/catalog/product/view/id/1316882026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08297-1-slirp-primary-antibodies-wb-testing-1.jpgAnti-SLIRP Antibody Picoband®Western blot analysis of SLIRP using anti-SLIRP antibody (A08297-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLIRP antigen affinity purified polyclonal antibody (A08297-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLIRP at approximately 15 kDa. The expected band size for SLIRP is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08297-1-slirp-primary-antibodies-ihc-testing-1.jpgAnti-SLIRP Antibody Picoband®IHC analysis of SLIRP using anti-SLIRP antibody (A08297-1). SLIRP was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLIRP Antibody (A08297-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08297-1-slirp-primary-antibodies-ihc-testing-2.jpgAnti-SLIRP Antibody Picoband®IHC analysis of SLIRP using anti-SLIRP antibody (A08297-1). SLIRP was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLIRP Antibody (A08297-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08297-1-slirp-primary-antibodies-if-testing-1.jpgAnti-SLIRP Antibody Picoband®IF analysis of SLIRP using anti-SLIRP antibody (A08297-1). SLIRP was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLIRP Antibody (A08297-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08297-1-slirp-primary-antibodies-if-testing-2.jpgAnti-SLIRP Antibody Picoband®IF analysis of SLIRP using anti-SLIRP antibody (A08297-1). SLIRP was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLIRP Antibody (A08297-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08297-1-slirp-primary-antibodies-if-testing-3.jpgAnti-SLIRP Antibody Picoband®IF analysis of SLIRP using anti-SLIRP antibody (A08297-1). SLIRP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLIRP Antibody (A08297-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08297-1-slirp-primary-antibodies-fcm-testing-1.jpgAnti-SLIRP Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-SLIRP antibody (A08297-1). Overlay histogram showing A549 cells stained with A08297-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLIRP Antibody (A08297-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316892026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01967-2-mfsd2a-primary-antibodies-wb-testing-1.jpgAnti-MFSD2A Antibody Picoband®Western blot analysis of MFSD2A using anti-MFSD2A antibody (A01967-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFSD2A antigen affinity purified polyclonal antibody (A01967-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MFSD2A at approximately 60 kDa. The expected band size for MFSD2A is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01967-2-mfsd2a-primary-antibodies-ihc-testing-1.jpgAnti-MFSD2A Antibody Picoband®IHC analysis of MFSD2A using anti-MFSD2A antibody (A01967-2). MFSD2A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MFSD2A Antibody (A01967-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01967-2-mfsd2a-primary-antibodies-ihc-testing-2.jpgAnti-MFSD2A Antibody Picoband®IHC analysis of MFSD2A using anti-MFSD2A antibody (A01967-2). MFSD2A was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MFSD2A Antibody (A01967-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01967-2-mfsd2a-primary-antibodies-if-testing-1.jpgAnti-MFSD2A Antibody Picoband®IF analysis of MFSD2A using anti-MFSD2A antibody (A01967-2). MFSD2A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MFSD2A Antibody (A01967-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01967-2-mfsd2a-primary-antibodies-if-testing-2.jpgAnti-MFSD2A Antibody Picoband®IF analysis of MFSD2A using anti-MFSD2A antibody (A01967-2). MFSD2A was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MFSD2A Antibody (A01967-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1316902026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11803-1-mpeg1-primary-antibodies-wb-testing-1.jpgAnti-MPEG1 Antibody Picoband®Western blot analysis of MPEG1 using anti-MPEG1 antibody (A11803-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPEG1 antigen affinity purified polyclonal antibody (A11803-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MPEG1 at approximately 79 kDa. The expected band size for MPEG1 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11803-1-mpeg1-primary-antibodies-fcm-testing-1.jpgAnti-MPEG1 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-MPEG1 antibody (A11803-1). Overlay histogram showing THP-1 cells stained with A11803-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MPEG1 Antibody (A11803-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316912026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03910-1-msl1-primary-antibodies-wb-testing-1.jpgAnti-MSL1 Antibody Picoband®Western blot analysis of MSL1 using anti-MSL1 antibody (A03910-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSL1 antigen affinity purified polyclonal antibody (A03910-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MSL1 at approximately 67 kDa. The expected band size for MSL1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03910-1-msl1-primary-antibodies-ihc-testing-1.jpgAnti-MSL1 Antibody Picoband®IHC analysis of MSL1 using anti-MSL1 antibody (A03910-1). MSL1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSL1 Antibody (A03910-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03910-1-msl1-primary-antibodies-if-testing-1.jpgAnti-MSL1 Antibody Picoband®IF analysis of MSL1 using anti-MSL1 antibody (A03910-1) and anti-Beta Tubulin antibody (M01857-3). MSL1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MSL1 Antibody (A03910-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03910-1-msl1-primary-antibodies-fcm-testing-1.jpgAnti-MSL1 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-MSL1 antibody (A03910-1). Overlay histogram showing K562 cells stained with A03910-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSL1 Antibody (A03910-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316922026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14323-1-siglec15-primary-antibodies-wb-testing-1.jpgAnti-SIGLEC15 Antibody Picoband®Western blot analysis of SIGLEC15 using anti-SIGLEC15 antibody (A14323-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIGLEC15 antigen affinity purified polyclonal antibody (A14323-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SIGLEC15 at approximately 40 kDa. The expected band size for SIGLEC15 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14323-1-siglec15-primary-antibodies-if-testing-1.jpgAnti-SIGLEC15 Antibody Picoband®IF analysis of SIGLEC15 using anti-SIGLEC15 antibody (A14323-1) and anti-Beta Tubulin antibody (M01857-3). SIGLEC15 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SIGLEC15 Antibody (A14323-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14323-1-siglec15-primary-antibodies-fcm-testing-1.jpgAnti-SIGLEC15 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-SIGLEC15 antibody (A14323-1). Overlay histogram showing A549 cells stained with A14323-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SIGLEC15 Antibody (A14323-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14323-1-siglec15-primary-antibodies-fcm-testing-2.jpgAnti-SIGLEC15 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-SIGLEC15 antibody (A14323-1). Overlay histogram showing THP-1 cells stained with A14323-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SIGLEC15 Antibody (A14323-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316932026-03-17T05:17:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12266-1-parp15-primary-antibodies-wb-testing-1.jpgAnti-PARP15 Antibody Picoband®Western blot analysis of PARP15 using anti-PARP15 antibody (A12266-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP15 antigen affinity purified polyclonal antibody (A12266-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PARP15 at approximately 50 kDa. The expected band size for PARP15 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12266-1-parp15-primary-antibodies-ihc-testing-1.jpgAnti-PARP15 Antibody Picoband®IHC analysis of PARP15 using anti-PARP15 antibody (A12266-1). PARP15 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARP15 Antibody (A12266-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12266-1-parp15-primary-antibodies-ihc-testing-2.jpgAnti-PARP15 Antibody Picoband®IHC analysis of PARP15 using anti-PARP15 antibody (A12266-1). PARP15 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARP15 Antibody (A12266-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12266-1-parp15-primary-antibodies-if-testing-1.jpgAnti-PARP15 Antibody Picoband®IF analysis of PARP15 using anti-PARP15 antibody (A12266-1) and anti-Beta Tubulin antibody (M01857-3). PARP15 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PARP15 Antibody (A12266-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12266-1-parp15-primary-antibodies-fcm-testing-1.jpgAnti-PARP15 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-PARP15 antibody (A12266-1). Overlay histogram showing Jurkat cells stained with A12266-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARP15 Antibody (A12266-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316942026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07476-1-snrk-primary-antibodies-wb-testing-1.jpgAnti-SNRK Antibody Picoband®Western blot analysis of SNRK using anti-SNRK antibody (A07476-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRK antigen affinity purified polyclonal antibody (A07476-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNRK at approximately 84 kDa. The expected band size for SNRK is at 84 kDa. https://www.bosterbio.com/catalog/product/view/id/1316952026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00288-1-son-primary-antibodies-wb-testing-1.jpgAnti-SON Antibody Picoband®Western blot analysis of SON using anti-SON antibody (A00288-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SON antigen affinity purified polyclonal antibody (A00288-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SON at approximately 264 kDa. The expected band size for SON is at 264 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00288-1-son-primary-antibodies-ihc-testing-1.jpgAnti-SON Antibody Picoband®IHC analysis of SON using anti-SON antibody (A00288-1). SON was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SON Antibody (A00288-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00288-1-son-primary-antibodies-ihc-testing-2.jpgAnti-SON Antibody Picoband®IHC analysis of SON using anti-SON antibody (A00288-1). SON was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SON Antibody (A00288-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00288-1-son-primary-antibodies-ihc-testing-3.jpgAnti-SON Antibody Picoband®IHC analysis of SON using anti-SON antibody (A00288-1). SON was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SON Antibody (A00288-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00288-1-son-primary-antibodies-if-testing-1.jpgAnti-SON Antibody Picoband®IF analysis of SON using anti-SON antibody (A00288-1). SON was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SON Antibody (A00288-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00288-1-son-primary-antibodies-if-testing-2.jpgAnti-SON Antibody Picoband®IF analysis of SON using anti-SON antibody (A00288-1) and anti-Beta Tubulin antibody (M01857-3). SON was detected in an immunocytochemical section of Siha cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SON Antibody (A00288-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00288-1-son-primary-antibodies-fcm-testing-1.jpgAnti-SON Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-SON antibody (A00288-1). Overlay histogram showing K562 cells stained with A00288-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SON Antibody (A00288-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316962026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15320-1-spata17-primary-antibodies-wb-testing-1.jpgAnti-SPATA17 Antibody Picoband®Western blot analysis of SPATA17 using anti-SPATA17 antibody (A15320-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA17 antigen affinity purified polyclonal antibody (A15320-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPATA17 at approximately 43 kDa. The expected band size for SPATA17 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15320-1-spata17-primary-antibodies-fcm-testing-1.jpgAnti-SPATA17 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-SPATA17 antibody (A15320-1). Overlay histogram showing PC-3 cells stained with A15320-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPATA17 Antibody (A15320-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316972026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05269-1-ostm1-primary-antibodies-wb-testing-1.jpgAnti-OSTM1 Antibody Picoband®Western blot analysis of OSTM1 using anti-OSTM1 antibody (A05269-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSTM1 antigen affinity purified polyclonal antibody (A05269-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OSTM1 at approximately 37 kDa. The expected band size for OSTM1 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05269-1-ostm1-primary-antibodies-if-testing-1.jpgAnti-OSTM1 Antibody Picoband®IF analysis of OSTM1 using anti-OSTM1 antibody (A05269-1) and anti-Beta Tubulin antibody (M01857-3). OSTM1 was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-OSTM1 Antibody (A05269-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1316982026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07497-2-p2rx1-primary-antibodies-wb-testing-1.jpgAnti-P2RX1 Antibody Picoband®Western blot analysis of P2RX1 using anti-P2RX1 antibody (A07497-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2RX1 antigen affinity purified polyclonal antibody (A07497-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P2RX1 at approximately 45 kDa. The expected band size for P2RX1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07497-2-p2rx1-primary-antibodies-if-testing-1.jpgAnti-P2RX1 Antibody Picoband®IF analysis of P2RX1 using anti-P2RX1 antibody (A07497-2). P2RX1 was detected in a paraffin-embedded section of rat bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-P2RX1 Antibody (A07497-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07497-2-p2rx1-primary-antibodies-if-testing-2.jpgAnti-P2RX1 Antibody Picoband®IF analysis of P2RX1 using anti-P2RX1 antibody (A07497-2). P2RX1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-P2RX1 Antibody (A07497-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07497-2-p2rx1-primary-antibodies-if-testing-3.jpgAnti-P2RX1 Antibody Picoband®IF analysis of P2RX1 using anti-P2RX1 antibody (A07497-2). P2RX1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-P2RX1 Antibody (A07497-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07497-2-p2rx1-primary-antibodies-if-testing-4.jpgAnti-P2RX1 Antibody Picoband®IF analysis of P2RX1 using anti-P2RX1 antibody (A07497-2). P2RX1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-P2RX1 Antibody (A07497-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07497-2-p2rx1-primary-antibodies-fcm-testing-1.jpgAnti-P2RX1 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-P2RX1 antibody (A07497-2). Overlay histogram showing THP-1 cells stained with A07497-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-P2RX1 Antibody (A07497-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1316992026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04413-3-msra-primary-antibodies-wb-testing-1.jpgAnti-MSRA Antibody Picoband®Western blot analysis of MSRA using anti-MSRA antibody (A04413-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSRA antigen affinity purified polyclonal antibody (A04413-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MSRA at approximately 24 kDa. The expected band size for MSRA is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04413-3-msra-primary-antibodies-fcm-testing-1.jpgAnti-MSRA Antibody Picoband®Flow Cytometry analysis of HEPA1-6 cells using anti-MSRA antibody (A04413-3). Overlay histogram showing HEPA1-6 cells stained with A04413-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSRA Antibody (A04413-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317002026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03182-1-ncr3-primary-antibodies-wb-testing-1.jpgAnti-NCR3 Antibody Picoband®Western blot analysis of NCR3 using anti-NCR3 antibody (A03182-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCR3 antigen affinity purified polyclonal antibody (A03182-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NCR3 at approximately 22 kDa. The expected band size for NCR3 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03182-1-ncr3-primary-antibodies-ihc-testing-1.jpgAnti-NCR3 Antibody Picoband®IHC analysis of NCR3 using anti-NCR3 antibody (A03182-1). NCR3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NCR3 Antibody (A03182-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1317012026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03254-2-spink5-primary-antibodies-wb-testing-1.jpgAnti-SPINK5 Antibody Picoband®Western blot analysis of SPINK5 using anti-SPINK5 antibody (A03254-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse stomach tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPINK5 antigen affinity purified polyclonal antibody (A03254-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPINK5 at approximately 120 kDa. The expected band size for SPINK5 is at 121 kDa. https://www.bosterbio.com/catalog/product/view/id/1317022026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06588-1-spock1-primary-antibodies-wb-testing-1.jpgAnti-SPOCK1 Antibody Picoband®Western blot analysis of SPOCK1 using anti-SPOCK1 antibody (A06588-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPOCK1 antigen affinity purified polyclonal antibody (A06588-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPOCK1 at approximately 49-56 kDa. The expected band size for SPOCK1 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06588-1-spock1-primary-antibodies-ihc-testing-1.jpgAnti-SPOCK1 Antibody Picoband®IHC analysis of SPOCK1 using anti-SPOCK1 antibody (A06588-1). SPOCK1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPOCK1 Antibody (A06588-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06588-1-spock1-primary-antibodies-ihc-testing-2.jpgAnti-SPOCK1 Antibody Picoband®IHC analysis of SPOCK1 using anti-SPOCK1 antibody (A06588-1). SPOCK1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPOCK1 Antibody (A06588-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06588-1-spock1-primary-antibodies-if-testing-1.jpgAnti-SPOCK1 Antibody Picoband®IF analysis of SPOCK1 using anti-SPOCK1 antibody (A06588-1). SPOCK1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SPOCK1 Antibody (A06588-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06588-1-spock1-primary-antibodies-fcm-testing-1.jpgAnti-SPOCK1 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-SPOCK1 antibody (A06588-1). Overlay histogram showing U251 cells stained with A06588-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SPOCK1 Antibody (A06588-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317032026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10454-1-srpx-primary-antibodies-wb-testing-1.jpgAnti-SRPX Antibody Picoband®Western blot analysis of SRPX using anti-SRPX antibody (A10454-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRPX antigen affinity purified polyclonal antibody (A10454-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SRPX at approximately 65-70 kDa. The expected band size for SRPX is at 52 kDa. https://www.bosterbio.com/catalog/product/view/id/1317042026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05370-3-snta1-primary-antibodies-wb-testing-1.jpgAnti-SNTA1 Antibody Picoband®Western blot analysis of SNTA1 using anti-SNTA1 antibody (A05370-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNTA1 antigen affinity purified polyclonal antibody (A05370-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNTA1 at approximately 54 kDa. The expected band size for SNTA1 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05370-3-snta1-primary-antibodies-fcm-testing-1.jpgAnti-SNTA1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-SNTA1 antibody (A05370-3). Overlay histogram showing HepG2 cells stained with A05370-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNTA1 Antibody (A05370-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317052026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04493-1-snx2-primary-antibodies-wb-testing-1.jpgAnti-SNX2 Antibody Picoband®Western blot analysis of SNX2 using anti-SNX2 antibody (A04493-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX2 antigen affinity purified polyclonal antibody (A04493-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNX2 at approximately 68 kDa. The expected band size for SNX2 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04493-1-snx2-primary-antibodies-ihc-testing-1.jpgAnti-SNX2 Antibody Picoband®IHC analysis of SNX2 using anti-SNX2 antibody (A04493-1). SNX2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX2 Antibody (A04493-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04493-1-snx2-primary-antibodies-ihc-testing-2.jpgAnti-SNX2 Antibody Picoband®IHC analysis of SNX2 using anti-SNX2 antibody (A04493-1). SNX2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX2 Antibody (A04493-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04493-1-snx2-primary-antibodies-fcm-testing-1.jpgAnti-SNX2 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-SNX2 antibody (A04493-1). Overlay histogram showing MCF-7 cells stained with A04493-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SNX2 Antibody (A04493-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317062026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13724-1-snx20-primary-antibodies-wb-testing-1.jpgAnti-SNX20 Antibody Picoband®Western blot analysis of SNX20 using anti-SNX20 antibody (A13724-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX20 antigen affinity purified polyclonal antibody (A13724-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNX20 at approximately 36 kDa. The expected band size for SNX20 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13724-1-snx20-primary-antibodies-fcm-testing-1.jpgAnti-SNX20 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-SNX20 antibody (A13724-1). Overlay histogram showing Jurkat cells stained with A13724-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SNX20 Antibody (A13724-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317072026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14863-1-spata6-primary-antibodies-wb-testing-1.jpgAnti-SPATA6 Antibody Picoband®Western blot analysis of SPATA6 using anti-SPATA6 antibody (A14863-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat ovary tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA6 antigen affinity purified polyclonal antibody (A14863-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPATA6 at approximately 56 kDa. The expected band size for SPATA6 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14863-1-spata6-primary-antibodies-ihc-testing-1.jpgAnti-SPATA6 Antibody Picoband®IHC analysis of SPATA6 using anti-SPATA6 antibody (A14863-1). SPATA6 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPATA6 Antibody (A14863-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14863-1-spata6-primary-antibodies-ihc-testing-2.jpgAnti-SPATA6 Antibody Picoband®IHC analysis of SPATA6 using anti-SPATA6 antibody (A14863-1). SPATA6 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPATA6 Antibody (A14863-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14863-1-spata6-primary-antibodies-fcm-testing-1.jpgAnti-SPATA6 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-SPATA6 antibody (A14863-1). Overlay histogram showing U251 cells stained with A14863-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SPATA6 Antibody (A14863-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317082026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15198-2-slc46a3-primary-antibodies-ihc-testing-1.jpgAnti-SLC46A3 AntibodyIHC analysis of SLC46A3 using anti-SLC46A3 antibody (A15198-2). SLC46A3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC46A3 Antibody (A15198-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15198-2-slc46a3-primary-antibodies-ihc-testing-2.jpgAnti-SLC46A3 AntibodyIHC analysis of SLC46A3 using anti-SLC46A3 antibody (A15198-2). SLC46A3 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC46A3 Antibody (A15198-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15198-2-slc46a3-primary-antibodies-ihc-testing-3.jpgAnti-SLC46A3 AntibodyIHC analysis of SLC46A3 using anti-SLC46A3 antibody (A15198-2). SLC46A3 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC46A3 Antibody (A15198-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15198-2-slc46a3-primary-antibodies-ihc-testing-4.jpgAnti-SLC46A3 AntibodyIHC analysis of SLC46A3 using anti-SLC46A3 antibody (A15198-2). SLC46A3 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC46A3 Antibody (A15198-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15198-2-slc46a3-primary-antibodies-fcm-testing-1.jpgAnti-SLC46A3 AntibodyFlow Cytometry analysis of RT4 cells using anti-SLC46A3 antibody (A15198-2). Overlay histogram showing RT4 cells stained with A15198-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC46A3 Antibody (A15198-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317092026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11003-3-snx16-primary-antibodies-wb-testing-1.jpgAnti-SNX16 Antibody Picoband®Western blot analysis of SNX16 using anti-SNX16 antibody (A11003-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX16 antigen affinity purified polyclonal antibody (A11003-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNX16 at approximately 50 kDa. The expected band size for SNX16 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11003-3-snx16-primary-antibodies-ihc-testing-1.jpgAnti-SNX16 Antibody Picoband®IHC analysis of SNX16 using anti-SNX16 antibody (A11003-3). SNX16 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX16 Antibody (A11003-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11003-3-snx16-primary-antibodies-ihc-testing-2.jpgAnti-SNX16 Antibody Picoband®IHC analysis of SNX16 using anti-SNX16 antibody (A11003-3). SNX16 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX16 Antibody (A11003-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11003-3-snx16-primary-antibodies-ihc-testing-3.jpgAnti-SNX16 Antibody Picoband®IHC analysis of SNX16 using anti-SNX16 antibody (A11003-3). SNX16 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX16 Antibody (A11003-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11003-3-snx16-primary-antibodies-ihc-testing-4.jpgAnti-SNX16 Antibody Picoband®IHC analysis of SNX16 using anti-SNX16 antibody (A11003-3). SNX16 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNX16 Antibody (A11003-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11003-3-snx16-primary-antibodies-if-testing-1.jpgAnti-SNX16 Antibody Picoband®IF analysis of SNX16 using anti-SNX16 antibody (A11003-3). SNX16 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNX16 Antibody (A11003-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11003-3-snx16-primary-antibodies-fcm-testing-1.jpgAnti-SNX16 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-SNX16 antibody (A11003-3). Overlay histogram showing MCF-7 cells stained with A11003-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNX16 Antibody (A11003-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317102026-03-17T05:17:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03429-1-snx27-primary-antibodies-wb-testing-1.jpgAnti-SNX27 Antibody Picoband®Western blot analysis of SNX27 using anti-SNX27 antibody (A03429-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX27 antigen affinity purified polyclonal antibody (A03429-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNX27 at approximately 61 kDa. The expected band size for SNX27 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03429-1-snx27-primary-antibodies-fcm-testing-1.jpgAnti-SNX27 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-SNX27 antibody (A03429-1). Overlay histogram showing Jurkat cells stained with A03429-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNX27 Antibody (A03429-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317112026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03354-2-sp110-primary-antibodies-wb-testing-1.jpgAnti-SP110 Antibody Picoband®Western blot analysis of SP110 using anti-SP110 antibody (A03354-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP110 antigen affinity purified polyclonal antibody (A03354-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SP110 at approximately 80 kDa. The expected band size for SP110 is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03354-2-sp110-primary-antibodies-ihc-testing-1.jpgAnti-SP110 Antibody Picoband®IHC analysis of SP110 using anti-SP110 antibody (A03354-2). SP110 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SP110 Antibody (A03354-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03354-2-sp110-primary-antibodies-fcm-testing-1.jpgAnti-SP110 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-SP110 antibody (A03354-2). Overlay histogram showing HEL cells stained with A03354-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SP110 Antibody (A03354-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317122026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06079-1-sycp1-primary-antibodies-wb-testing-1.jpgAnti-SYCP1 Antibody Picoband®Western blot analysis of SYCP1 using anti-SYCP1 antibody (A06079-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse TM4 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYCP1 antigen affinity purified polyclonal antibody (A06079-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYCP1 at approximately 120 kDa. The expected band size for SYCP1 is at 114 kDa. https://www.bosterbio.com/catalog/product/view/id/1317132026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05345-1-stx4-primary-antibodies-wb-testing-1.jpgAnti-Syntaxin 4/STX4 Antibody Picoband®Western blot analysis of Syntaxin 4/STX4 using anti-Syntaxin 4/STX4 antibody (A05345-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat RH35 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 4/STX4 antigen affinity purified polyclonal antibody (A05345-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Syntaxin 4/STX4 at approximately 37 kDa. The expected band size for Syntaxin 4/STX4 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05345-1-stx4-primary-antibodies-ihc-testing-1.jpgAnti-Syntaxin 4/STX4 Antibody Picoband®IHC analysis of Syntaxin 4/STX4 using anti-Syntaxin 4/STX4 antibody (A05345-1). Syntaxin 4/STX4 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntaxin 4/STX4 Antibody (A05345-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05345-1-stx4-primary-antibodies-ihc-testing-2.jpgAnti-Syntaxin 4/STX4 Antibody Picoband®IHC analysis of Syntaxin 4/STX4 using anti-Syntaxin 4/STX4 antibody (A05345-1). Syntaxin 4/STX4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Syntaxin 4/STX4 Antibody (A05345-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05345-1-stx4-primary-antibodies-if-testing-1.jpgAnti-Syntaxin 4/STX4 Antibody Picoband®IF analysis of Syntaxin 4/STX4 using anti-Syntaxin 4/STX4 antibody (A05345-1). Syntaxin 4/STX4 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Syntaxin 4/STX4 Antibody (A05345-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05345-1-stx4-primary-antibodies-ip-testing-1.jpgAnti-Syntaxin 4/STX4 Antibody Picoband®Immunoprecipitating Syntaxin 4/STX4 in jurkat whole cell lysate. Western blot analysis of Syntaxin 4/STX4 using anti-Syntaxin 4/STX4 antibody (A05345-1). Lane 1: jurkat whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-Syntaxin 4/STX4 antibody in jurkat whole cell lysate, Lane 3: anti-Syntaxin 4/STX4 antibody (2μg) + jurkat whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Syntaxin 4/STX4 antigen affinity purified polyclonal antibody (A05345-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Syntaxin 4/STX4 at approximately 37 kDa. The expected band size for Syntaxin 4/STX4 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05345-1-stx4-primary-antibodies-fcm-testing-1.jpgAnti-Syntaxin 4/STX4 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-Syntaxin 4/STX4 antibody (A05345-1). Overlay histogram showing K562 cells stained with A05345-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Syntaxin 4/STX4 Antibody (A05345-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317142026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09705-stx5-primary-antibodies-wb-testing-1.jpgAnti-STX5 Antibody Picoband®Western blot analysis of STX5 using anti-STX5 antibody (A09705). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse 3T3-L1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STX5 antigen affinity purified polyclonal antibody (A09705) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STX5 at approximately 37 kDa. The expected band size for STX5 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09705-stx5-primary-antibodies-ihc-testing-1.jpgAnti-STX5 Antibody Picoband®IHC analysis of STX5 using anti-STX5 antibody (A09705). STX5 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STX5 Antibody (A09705) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09705-stx5-primary-antibodies-ihc-testing-2.jpgAnti-STX5 Antibody Picoband®IHC analysis of STX5 using anti-STX5 antibody (A09705). STX5 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STX5 Antibody (A09705) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09705-stx5-primary-antibodies-if-testing-1.jpgAnti-STX5 Antibody Picoband®IF analysis of STX5 using anti-STX5 antibody (A09705). STX5 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-STX5 Antibody (A09705) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09705-stx5-primary-antibodies-if-testing-2.jpgAnti-STX5 Antibody Picoband®IF analysis of STX5 using anti-STX5 antibody (A09705). STX5 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-STX5 Antibody (A09705) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09705-stx5-primary-antibodies-if-testing-3.jpgAnti-STX5 Antibody Picoband®IF analysis of STX5 using anti-STX5 antibody (A09705). STX5 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STX5 Antibody (A09705) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09705-stx5-primary-antibodies-fcm-testing-1.jpgAnti-STX5 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-STX5 antibody (A09705). Overlay histogram showing 293T cells stained with A09705 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STX5 Antibody (A09705, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317152026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14108-2-syt10-primary-antibodies-wb-testing-1.jpgAnti-Synaptotagmin-10/SYT10 Antibody Picoband®Western blot analysis of Synaptotagmin-10/SYT10 using anti-Synaptotagmin-10/SYT10 antibody (A14108-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptotagmin-10/SYT10 antigen affinity purified polyclonal antibody (A14108-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Synaptotagmin-10/SYT10 at approximately 59 kDa. The expected band size for Synaptotagmin-10/SYT10 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14108-2-syt10-primary-antibodies-ihc-testing-1.jpgAnti-Synaptotagmin-10/SYT10 Antibody Picoband®IHC analysis of Synaptotagmin-10/SYT10 using anti-Synaptotagmin-10/SYT10 antibody (A14108-2). Synaptotagmin-10/SYT10 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synaptotagmin-10/SYT10 Antibody (A14108-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14108-2-syt10-primary-antibodies-ihc-testing-2.jpgAnti-Synaptotagmin-10/SYT10 Antibody Picoband®IHC analysis of Synaptotagmin-10/SYT10 using anti-Synaptotagmin-10/SYT10 antibody (A14108-2). Synaptotagmin-10/SYT10 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synaptotagmin-10/SYT10 Antibody (A14108-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14108-2-syt10-primary-antibodies-ihc-testing-3.jpgAnti-Synaptotagmin-10/SYT10 Antibody Picoband®IHC analysis of Synaptotagmin-10/SYT10 using anti-Synaptotagmin-10/SYT10 antibody (A14108-2). Synaptotagmin-10/SYT10 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synaptotagmin-10/SYT10 Antibody (A14108-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14108-2-syt10-primary-antibodies-if-testing-1.jpgAnti-Synaptotagmin-10/SYT10 Antibody Picoband®IF analysis of Synaptotagmin-10/SYT10 using anti-Synaptotagmin-10/SYT10 antibody (A14108-2). Synaptotagmin-10/SYT10 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Synaptotagmin-10/SYT10 Antibody (A14108-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14108-2-syt10-primary-antibodies-if-testing-2.jpgAnti-Synaptotagmin-10/SYT10 Antibody Picoband®IF analysis of Synaptotagmin-10/SYT10 using anti-Synaptotagmin-10/SYT10 antibody (A14108-2). Synaptotagmin-10/SYT10 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Synaptotagmin-10/SYT10 Antibody (A14108-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14108-2-syt10-primary-antibodies-if-testing-3.jpgAnti-Synaptotagmin-10/SYT10 Antibody Picoband®IF analysis of Synaptotagmin-10/SYT10 using anti-Synaptotagmin-10/SYT10 antibody (A14108-2). Synaptotagmin-10/SYT10 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Synaptotagmin-10/SYT10 Antibody (A14108-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14108-2-syt10-primary-antibodies-if-testing-4.jpgAnti-Synaptotagmin-10/SYT10 Antibody Picoband®IF analysis of Synaptotagmin-10/SYT10 using anti-Synaptotagmin-10/SYT10 antibody (A14108-2). Synaptotagmin-10/SYT10 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Synaptotagmin-10/SYT10 Antibody (A14108-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1317162026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08618-2-taf5-primary-antibodies-wb-testing-1.jpgAnti-TAF5 Antibody Picoband®Western blot analysis of TAF5 using anti-TAF5 antibody (A08618-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF5 antigen affinity purified polyclonal antibody (A08618-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAF5 at approximately 100 kDa. The expected band size for TAF5 is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08618-2-taf5-primary-antibodies-ihc-testing-1.jpgAnti-TAF5 Antibody Picoband®IHC analysis of TAF5 using anti-TAF5 antibody (A08618-2). TAF5 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TAF5 Antibody (A08618-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08618-2-taf5-primary-antibodies-if-testing-1.jpgAnti-TAF5 Antibody Picoband®IF analysis of TAF5 using anti-TAF5 antibody (A08618-2). TAF5 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TAF5 Antibody (A08618-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08618-2-taf5-primary-antibodies-ip-testing-1.jpgAnti-TAF5 Antibody Picoband®Immunoprecipitating TAF5 in Hela whole cell lysate. Western blot analysis of TAF5 using anti-TAF5 antibody (A08618-2). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TAF5 antibody in Hela whole cell lysate, Lane 3: anti-TAF5 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TAF5 antigen affinity purified polyclonal antibody (A08618-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TAF5 at approximately 100 kDa. The expected band size for TAF5 is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08618-2-taf5-primary-antibodies-fcm-testing-1.jpgAnti-TAF5 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-TAF5 antibody (A08618-2). Overlay histogram showing MCF-7 cells stained with A08618-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAF5 Antibody (A08618-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317172026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03567-1-taf15-primary-antibodies-wb-testing-1.jpgAnti-TAF15 Antibody Picoband®Western blot analysis of TAF15 using anti-TAF15 antibody (A03567-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAF15 antigen affinity purified polyclonal antibody (A03567-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAF15 at approximately 68 kDa. The expected band size for TAF15 is at 62 kDa. https://www.bosterbio.com/catalog/product/view/id/1317182026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12228-1-tesmin-primary-antibodies-wb-testing-1.jpgAnti-TESMIN Antibody Picoband®Western blot analysis of TESMIN using anti-TESMIN antibody (A12228-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TESMIN antigen affinity purified polyclonal antibody (A12228-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TESMIN at approximately 50 kDa. The expected band size for TESMIN is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12228-1-tesmin-primary-antibodies-ihc-testing-1.jpgAnti-TESMIN Antibody Picoband®IHC analysis of TESMIN using anti-TESMIN antibody (A12228-1). TESMIN was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TESMIN Antibody (A12228-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12228-1-tesmin-primary-antibodies-fcm-testing-1.jpgAnti-TESMIN Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-TESMIN antibody (A12228-1). Overlay histogram showing MCF-7 cells stained with A12228-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TESMIN Antibody (A12228-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317192026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09854-2-hexim2-primary-antibodies-wb-testing-1_1.jpgAnti-HEXIM2 Antibody Picoband®Western blot analysis of HEXIM2 using anti-HEXIM2 antibody (A09854-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COL0320 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEXIM2 antigen affinity purified polyclonal antibody (A09854-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HEXIM2 at approximately 38 kDa. The expected band size for HEXIM2 is at 35 kDa. https://www.bosterbio.com/catalog/product/view/id/1317202026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02374-1-terf2ip-primary-antibodies-wb-testing-1.jpgAnti-TERF2IP Antibody Picoband®Western blot analysis of TERF2IP using anti-TERF2IP antibody (A02374-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HT1080 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TERF2IP antigen affinity purified polyclonal antibody (A02374-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TERF2IP at approximately 60 kDa. The expected band size for TERF2IP is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02374-1-terf2ip-primary-antibodies-ihc-testing-1.jpgAnti-TERF2IP Antibody Picoband®IHC analysis of TERF2IP using anti-TERF2IP antibody (A02374-1). TERF2IP was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TERF2IP Antibody (A02374-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02374-1-terf2ip-primary-antibodies-ihc-testing-2.jpgAnti-TERF2IP Antibody Picoband®IHC analysis of TERF2IP using anti-TERF2IP antibody (A02374-1). TERF2IP was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TERF2IP Antibody (A02374-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02374-1-terf2ip-primary-antibodies-if-testing-1.jpgAnti-TERF2IP Antibody Picoband®IF analysis of TERF2IP using anti-TERF2IP antibody (A02374-1) and anti-Beta Tubulin antibody (M01857-3). TERF2IP was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TERF2IP Antibody (A02374-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1317212026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07976-1-tfap4-primary-antibodies-wb-testing-1.jpgAnti-TFAP4 Antibody Picoband®Western blot analysis of TFAP4 using anti-TFAP4 antibody (A07976-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFAP4 antigen affinity purified polyclonal antibody (A07976-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TFAP4 at approximately 40 kDa. The expected band size for TFAP4 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07976-1-tfap4-primary-antibodies-if-testing-1.jpgAnti-TFAP4 Antibody Picoband®IF analysis of TFAP4 using anti-TFAP4 antibody (A07976-1) and anti-Beta Tubulin antibody (M01857-3). TFAP4 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TFAP4 Antibody (A07976-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07976-1-tfap4-primary-antibodies-fcm-testing-1.jpgAnti-TFAP4 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-TFAP4 antibody (A07976-1). Overlay histogram showing Jurkat cells stained with A07976-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFAP4 Antibody (A07976-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317222026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11396-3-tfdp3-primary-antibodies-wb-testing-1.jpgAnti-TFDP3 Antibody Picoband®Western blot analysis of TFDP3 using anti-TFDP3 antibody (A11396-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human HCT116 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFDP3 antigen affinity purified polyclonal antibody (A11396-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TFDP3 at approximately 45 kDa. The expected band size for TFDP3 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11396-3-tfdp3-primary-antibodies-ihc-testing-1.jpgAnti-TFDP3 Antibody Picoband®IHC analysis of TFDP3 using anti-TFDP3 antibody (A11396-3). TFDP3 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFDP3 Antibody (A11396-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11396-3-tfdp3-primary-antibodies-if-testing-1.jpgAnti-TFDP3 Antibody Picoband®IF analysis of TFDP3 using anti-TFDP3 antibody (A11396-3). TFDP3 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TFDP3 Antibody (A11396-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11396-3-tfdp3-primary-antibodies-if-testing-2.jpgAnti-TFDP3 Antibody Picoband®IF analysis of TFDP3 using anti-TFDP3 antibody (A11396-3) and anti-Beta Tubulin antibody (M01857-3). TFDP3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TFDP3 Antibody (A11396-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1317232026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07310-1-thrsp-primary-antibodies-wb-testing-1.jpgAnti-THRSP Antibody Picoband®Western blot analysis of THRSP using anti-THRSP antibody (A07310-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THRSP antigen affinity purified polyclonal antibody (A07310-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for THRSP at approximately 17 kDa. The expected band size for THRSP is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07310-1-thrsp-primary-antibodies-fcm-testing-1.jpgAnti-THRSP Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-THRSP antibody (A07310-1). Overlay histogram showing MCF-7 cells stained with A07310-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-THRSP Antibody (A07310-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317242026-03-17T05:17:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07841-3-tfec-primary-antibodies-wb-testing-1.jpgAnti-TFEC Antibody Picoband®Western blot analysis of TFEC using anti-TFEC antibody (A07841-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFEC antigen affinity purified polyclonal antibody (A07841-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TFEC at approximately 39 kDa. The expected band size for TFEC is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07841-3-tfec-primary-antibodies-if-testing-1.jpgAnti-TFEC Antibody Picoband®IF analysis of TFEC using anti-TFEC antibody (A07841-3) and anti-Beta Tubulin antibody (M01857-3). TFEC was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TFEC Antibody (A07841-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07841-3-tfec-primary-antibodies-fcm-testing-1.jpgAnti-TFEC Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-TFEC antibody (A07841-3). Overlay histogram showing THP-1 cells stained with A07841-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFEC Antibody (A07841-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317252026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08499-2-tgif2-primary-antibodies-wb-testing-1.jpgAnti-TGIF2 Antibody Picoband®Western blot analysis of TGIF2 using anti-TGIF2 antibody (A08499-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGIF2 antigen affinity purified polyclonal antibody (A08499-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TGIF2 at approximately 30 kDa. The expected band size for TGIF2 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08499-2-tgif2-primary-antibodies-if-testing-1.jpgAnti-TGIF2 Antibody Picoband®IF analysis of TGIF2 using anti-TGIF2 antibody (A08499-2) and anti-Beta Tubulin antibody (M01857-3). TGIF2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TGIF2 Antibody (A08499-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08499-2-tgif2-primary-antibodies-ip-testing-1.jpgAnti-TGIF2 Antibody Picoband®Immunoprecipitating TGIF2 in Jurkat whole cell lysate. Western blot analysis of TGIF2 using anti-TGIF2 antibody (A08499-2). Lane 1: Jurkat whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TGIF2 antibody in Jurkat whole cell lysate, Lane 3: anti-TGIF2 antibody (2μg) + Jurkat whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TGIF2 antigen affinity purified polyclonal antibody (A08499-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TGIF2 at approximately 30 kDa. The expected band size for TGIF2 is at 26 kDa. https://www.bosterbio.com/catalog/product/view/id/1317262026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09912-1-tgm4-primary-antibodies-wb-testing-1.jpgAnti-TGM4 Antibody Picoband®Western blot analysis of TGM4 using anti-TGM4 antibody (A09912-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse RM-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGM4 antigen affinity purified polyclonal antibody (A09912-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TGM4 at approximately 68 kDa. The expected band size for TGM4 is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09912-1-tgm4-primary-antibodies-fcm-testing-1.jpgAnti-TGM4 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-TGM4 antibody (A09912-1). Overlay histogram showing SH-SY5Y cells stained with A09912-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TGM4 Antibody (A09912-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317272026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18282-2-tgm7-primary-antibodies-wb-testing-1.jpgAnti-TGM7 Antibody Picoband®Western blot analysis of TGM7 using anti-TGM7 antibody (A18282-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGM7 antigen affinity purified polyclonal antibody (A18282-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TGM7 at approximately 75 kDa. The expected band size for TGM7 is at 80 kDa. https://www.bosterbio.com/catalog/product/view/id/1317282026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04382-2-rbbp6-primary-antibodies-wb-testing-1.jpgAnti-RBBP6 Antibody Picoband®Western blot analysis of RBBP6 using anti-RBBP6 antibody (A04382-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBBP6 antigen affinity purified polyclonal antibody (A04382-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBBP6 at approximately 250 kDa. The expected band size for RBBP6 is at 202 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04382-2-rbbp6-primary-antibodies-ihc-testing-1.jpgAnti-RBBP6 Antibody Picoband®IHC analysis of RBBP6 using anti-RBBP6 antibody (A04382-2). RBBP6 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBBP6 Antibody (A04382-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04382-2-rbbp6-primary-antibodies-if-testing-1.jpgAnti-RBBP6 Antibody Picoband®IF analysis of RBBP6 using anti-RBBP6 antibody (A04382-2) and anti-Beta Tubulin antibody (M01857-3). RBBP6 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RBBP6 Antibody (A04382-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04382-2-rbbp6-primary-antibodies-fcm-testing-1.jpgAnti-RBBP6 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-RBBP6 antibody (A04382-2). Overlay histogram showing Jurkat cells stained with A04382-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBBP6 Antibody (A04382-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317292026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09161-1-npw-primary-antibodies-wb-testing-1.jpgAnti-NPW Antibody Picoband®Western blot analysis of NPW using anti-NPW antibody (A09161-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPW antigen affinity purified polyclonal antibody (A09161-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NPW at approximately 15 kDa. The expected band size for NPW is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09161-1-npw-primary-antibodies-fcm-testing-1.jpgAnti-NPW Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-NPW antibody (A09161-1). Overlay histogram showing HepG2 cells stained with A09161-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NPW Antibody (A09161-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317302026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08880-2-opn5-primary-antibodies-wb-testing-1.jpgAnti-OPN5 Antibody Picoband®Western blot analysis of OPN5 using anti-OPN5 antibody (A08880-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPN5 antigen affinity purified polyclonal antibody (A08880-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OPN5 at approximately 40 kDa. The expected band size for OPN5 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08880-2-opn5-primary-antibodies-ihc-testing-1.jpgAnti-OPN5 Antibody Picoband®IHC analysis of OPN5 using anti-OPN5 antibody (A08880-2). OPN5 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OPN5 Antibody (A08880-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08880-2-opn5-primary-antibodies-ihc-testing-2.jpgAnti-OPN5 Antibody Picoband®IHC analysis of OPN5 using anti-OPN5 antibody (A08880-2). OPN5 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OPN5 Antibody (A08880-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08880-2-opn5-primary-antibodies-if-testing-1.jpgAnti-OPN5 Antibody Picoband®IF analysis of OPN5 using anti-OPN5 antibody (A08880-2). OPN5 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-OPN5 Antibody (A08880-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1317312026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03667-1-tbc1d1-primary-antibodies-wb-testing-1.jpgAnti-TBC1D1 Antibody Picoband®Western blot analysis of TBC1D1 using anti-TBC1D1 antibody (A03667-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBC1D1 antigen affinity purified polyclonal antibody (A03667-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TBC1D1 at approximately 150 kDa. The expected band size for TBC1D1 is at 105 kDa. https://www.bosterbio.com/catalog/product/view/id/1317322026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10430-1-pecr-primary-antibodies-wb-testing-1.jpgAnti-PECR Antibody Picoband®Western blot analysis of PECR using anti-PECR antibody (A10430-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PECR antigen affinity purified polyclonal antibody (A10430-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PECR at approximately 33 kDa. The expected band size for PECR is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10430-1-pecr-primary-antibodies-ihc-testing-1.jpgAnti-PECR Antibody Picoband®IHC analysis of PECR using anti-PECR antibody (A10430-1). PECR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PECR Antibody (A10430-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10430-1-pecr-primary-antibodies-ihc-testing-2.jpgAnti-PECR Antibody Picoband®IHC analysis of PECR using anti-PECR antibody (A10430-1). PECR was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PECR Antibody (A10430-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10430-1-pecr-primary-antibodies-if-testing-1.jpgAnti-PECR Antibody Picoband®IF analysis of PECR using anti-PECR antibody (A10430-1). PECR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PECR Antibody (A10430-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1317332026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10984-1-tbkbp1-primary-antibodies-wb-testing-1.jpgAnti-TBKBP1 Antibody Picoband®Western blot analysis of TBKBP1 using anti-TBKBP1 antibody (A10984-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBKBP1 antigen affinity purified polyclonal antibody (A10984-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TBKBP1 at approximately 78 kDa. The expected band size for TBKBP1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10984-1-tbkbp1-primary-antibodies-if-testing-1.jpgAnti-TBKBP1 Antibody Picoband®IF analysis of TBKBP1 using anti-TBKBP1 antibody (A10984-1) and anti-Beta Tubulin antibody (M01857-3). TBKBP1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TBKBP1 Antibody (A10984-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10984-1-tbkbp1-primary-antibodies-fcm-testing-1.jpgAnti-TBKBP1 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-TBKBP1 antibody (A10984-1). Overlay histogram showing SH-SY5Y cells stained with A10984-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TBKBP1 Antibody (A10984-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317342026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04697-1-tbxas1-primary-antibodies-wb-testing-1.jpgAnti-TBXAS1 Antibody Picoband®Western blot analysis of TBXAS1 using anti-TBXAS1 antibody (A04697-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBXAS1 antigen affinity purified polyclonal antibody (A04697-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TBXAS1 at approximately 61 kDa. The expected band size for TBXAS1 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04697-1-tbxas1-primary-antibodies-if-testing-1.jpgAnti-TBXAS1 Antibody Picoband®IF analysis of TBXAS1 using anti-TBXAS1 antibody (A04697-1). TBXAS1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TBXAS1 Antibody (A04697-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1317352026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06679-1-tcf20-primary-antibodies-wb-testing-1.jpgAnti-TCF20 Antibody Picoband®Western blot analysis of TCF20 using anti-TCF20 antibody (A06679-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCF20 antigen affinity purified polyclonal antibody (A06679-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TCF20 at approximately 250 kDa. The expected band size for TCF20 is at 212 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06679-1-tcf20-primary-antibodies-if-testing-1.jpgAnti-TCF20 Antibody Picoband®IF analysis of TCF20 using anti-TCF20 antibody (A06679-1) and anti-Beta Tubulin antibody (M01857-3). TCF20 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TCF20 Antibody (A06679-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1317362026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-wb-testing-1_1.jpgAnti-PABPN1 Antibody Picoband®Western blot analysis of PABPN1 using anti-PABPN1 antibody (A02445-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PABPN1 antigen affinity purified polyclonal antibody (A02445-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PABPN1 at approximately 50 kDa. The expected band size for PABPN1 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-ihc-testing-1_1.jpgAnti-PABPN1 Antibody Picoband®IHC analysis of PABPN1 using anti-PABPN1 antibody (A02445-2). PABPN1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PABPN1 Antibody (A02445-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-ihc-testing-2_1.jpgAnti-PABPN1 Antibody Picoband®IHC analysis of PABPN1 using anti-PABPN1 antibody (A02445-2). PABPN1 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PABPN1 Antibody (A02445-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-ihc-testing-3_1.jpgAnti-PABPN1 Antibody Picoband®IHC analysis of PABPN1 using anti-PABPN1 antibody (A02445-2). PABPN1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PABPN1 Antibody (A02445-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-ihc-testing-4_1.jpgAnti-PABPN1 Antibody Picoband®IHC analysis of PABPN1 using anti-PABPN1 antibody (A02445-2). PABPN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PABPN1 Antibody (A02445-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-if-testing-1.jpgAnti-PABPN1 Antibody Picoband®IF analysis of PABPN1 using anti-PABPN1 antibody (A02445-2). PABPN1 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PABPN1 Antibody (A02445-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-if-testing-2.jpgAnti-PABPN1 Antibody Picoband®IF analysis of PABPN1 using anti-PABPN1 antibody (A02445-2). PABPN1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PABPN1 Antibody (A02445-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-if-testing-3.jpgAnti-PABPN1 Antibody Picoband®IF analysis of PABPN1 using anti-PABPN1 antibody (A02445-2). PABPN1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PABPN1 Antibody (A02445-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-if-testing-4.jpgAnti-PABPN1 Antibody Picoband®IF analysis of PABPN1 using anti-PABPN1 antibody (A02445-2). PABPN1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PABPN1 Antibody (A02445-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-if-testing-5.jpgAnti-PABPN1 Antibody Picoband®IF analysis of PABPN1 using anti-PABPN1 antibody (A02445-2). PABPN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PABPN1 Antibody (A02445-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-if-testing-6.jpgAnti-PABPN1 Antibody Picoband®IF analysis of PABPN1 using anti-PABPN1 antibody (A02445-2). PABPN1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PABPN1 Antibody (A02445-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-if-testing-7.jpgAnti-PABPN1 Antibody Picoband®IF analysis of PABPN1 using anti-PABPN1 antibody (A02445-2) and anti-Beta Tubulin antibody (M01857-3). PABPN1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PABPN1 Antibody (A02445-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02445-2-pabpn1-primary-antibodies-fcm-testing-1.jpgAnti-PABPN1 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-PABPN1 antibody (A02445-2). Overlay histogram showing MCF-7 cells stained with A02445-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PABPN1 Antibody (A02445-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317372026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16792-1-them6-primary-antibodies-wb-testing-1.jpgAnti-THEM6 Antibody Picoband®Western blot analysis of THEM6 using anti-THEM6 antibody (A16792-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THEM6 antigen affinity purified polyclonal antibody (A16792-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for THEM6 at approximately 20 kDa. The expected band size for THEM6 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16792-1-them6-primary-antibodies-if-testing-1.jpgAnti-THEM6 Antibody Picoband®IF analysis of THEM6 using anti-THEM6 antibody (A16792-1). THEM6 was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-THEM6 Antibody (A16792-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16792-1-them6-primary-antibodies-ip-testing-1.jpgAnti-THEM6 Antibody Picoband®Immunoprecipitating THEM6 in PC-3 whole cell lysate. Western blot analysis of THEM6 using anti-THEM6 antibody (A16792-1). Lane 1: PC-3 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-THEM6 antibody in PC-3 whole cell lysate, Lane 3: anti-THEM6 antibody (2μg) + PC-3 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-THEM6 antigen affinity purified polyclonal antibody (A16792-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for THEM6 at approximately 20 kDa. The expected band size for THEM6 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16792-1-them6-primary-antibodies-fcm-testing-1.jpgAnti-THEM6 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-THEM6 antibody (A16792-1). Overlay histogram showing MCF-7 cells stained with A16792-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-THEM6 Antibody (A16792-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317382026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08563-1-tgm5-primary-antibodies-wb-testing-1.jpgAnti-TGM5 Antibody Picoband®Western blot analysis of TGM5 using anti-TGM5 antibody (A08563-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGM5 antigen affinity purified polyclonal antibody (A08563-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TGM5 at approximately 81 kDa. The expected band size for TGM5 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08563-1-tgm5-primary-antibodies-fcm-testing-1.jpgAnti-TGM5 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-TGM5 antibody (A08563-1). Overlay histogram showing THP-1 cells stained with A08563-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TGM5 Antibody (A08563-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317392026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01871-3-sting-primary-antibodies-wb-testing-1.jpgAnti-TMEM173/STING Antibody Picoband®Western blot analysis of TMEM173/STING using anti-TMEM173/STING antibody (A01871-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse MH-S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM173/STING antigen affinity purified polyclonal antibody (A01871-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMEM173/STING at approximately 36 kDa. The expected band size for TMEM173/STING is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01871-3-sting-primary-antibodies-ihc-testing-1.jpgAnti-TMEM173/STING Antibody Picoband®IHC analysis of TMEM173/STING using anti-TMEM173/STING antibody (A01871-3). TMEM173/STING was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM173/STING Antibody (A01871-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01871-3-sting-primary-antibodies-if-testing-1.jpgAnti-TMEM173/STING Antibody Picoband®IF analysis of TMEM173/STING using anti-TMEM173/STING antibody (A01871-3) and anti-Beta Tubulin antibody (M01857-3). TMEM173/STING was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TMEM173/STING Antibody (A01871-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01871-3-sting-primary-antibodies-fcm-testing-1.jpgAnti-TMEM173/STING Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-TMEM173/STING antibody (A01871-3). Overlay histogram showing HepG2 cells stained with A01871-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM173/STING Antibody (A01871-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317402026-03-17T05:17:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11162-2-slc7a14-primary-antibodies-wb-testing-1.jpgAnti-SLC7A14 Antibody Picoband®Western blot analysis of SLC7A14 using anti-SLC7A14 antibody (A11162-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat hippocampus tissue lysates, Lane 2: mouse hippocampus tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC7A14 antigen affinity purified polyclonal antibody (A11162-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC7A14 at approximately 84 kDa. The expected band size for SLC7A14 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11162-2-slc7a14-primary-antibodies-ihc-testing-1.jpgAnti-SLC7A14 Antibody Picoband®IHC analysis of SLC7A14 using anti-SLC7A14 antibody (A11162-2). SLC7A14 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC7A14 Antibody (A11162-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11162-2-slc7a14-primary-antibodies-ihc-testing-2.jpgAnti-SLC7A14 Antibody Picoband®IHC analysis of SLC7A14 using anti-SLC7A14 antibody (A11162-2). SLC7A14 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC7A14 Antibody (A11162-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11162-2-slc7a14-primary-antibodies-ihc-testing-3.jpgAnti-SLC7A14 Antibody Picoband®IHC analysis of SLC7A14 using anti-SLC7A14 antibody (A11162-2). SLC7A14 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC7A14 Antibody (A11162-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11162-2-slc7a14-primary-antibodies-ihc-testing-4.jpgAnti-SLC7A14 Antibody Picoband®IHC analysis of SLC7A14 using anti-SLC7A14 antibody (A11162-2). SLC7A14 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC7A14 Antibody (A11162-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11162-2-slc7a14-primary-antibodies-if-testing-1.jpgAnti-SLC7A14 Antibody Picoband®IF analysis of SLC7A14 using anti-SLC7A14 antibody (A11162-2). SLC7A14 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLC7A14 Antibody (A11162-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11162-2-slc7a14-primary-antibodies-if-testing-2.jpgAnti-SLC7A14 Antibody Picoband®IF analysis of SLC7A14 using anti-SLC7A14 antibody (A11162-2). SLC7A14 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLC7A14 Antibody (A11162-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11162-2-slc7a14-primary-antibodies-fcm-testing-1.jpgAnti-SLC7A14 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-SLC7A14 antibody (A11162-2). Overlay histogram showing SH-SY5Y cells stained with A11162-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC7A14 Antibody (A11162-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317412026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10691-1-spsb2-primary-antibodies-wb-testing-1.jpgAnti-SPSB2 Antibody Picoband®Western blot analysis of SPSB2 using anti-SPSB2 antibody (A10691-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPSB2 antigen affinity purified polyclonal antibody (A10691-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPSB2 at approximately 29 kDa. The expected band size for SPSB2 is at 29 kDa. https://www.bosterbio.com/catalog/product/view/id/1317422026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03094-1-spon1-primary-antibodies-ihc-testing-1.jpgAnti-SPON1 AntibodyIHC analysis of SPON1 using anti-SPON1 antibody (A03094-1). SPON1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPON1 Antibody (A03094-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1317432026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03852-3-rest-primary-antibodies-wb-testing-1.jpgAnti-REST Antibody Picoband®Western blot analysis of REST using anti-REST antibody (A03852-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REST antigen affinity purified polyclonal antibody (A03852-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for REST at approximately 51 kDa. The expected band size for REST is at 122 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03852-3-rest-primary-antibodies-ihc-testing-1.jpgAnti-REST Antibody Picoband®IHC analysis of REST using anti-REST antibody (A03852-3). REST was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-REST Antibody (A03852-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03852-3-rest-primary-antibodies-ihc-testing-2.jpgAnti-REST Antibody Picoband®IHC analysis of REST using anti-REST antibody (A03852-3). REST was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-REST Antibody (A03852-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03852-3-rest-primary-antibodies-ihc-testing-3.jpgAnti-REST Antibody Picoband®IHC analysis of REST using anti-REST antibody (A03852-3). REST was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-REST Antibody (A03852-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03852-3-rest-primary-antibodies-fcm-testing-1.jpgAnti-REST Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-REST antibody (A03852-3). Overlay histogram showing K562 cells stained with A03852-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-REST Antibody (A03852-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1317442026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00373-3-cd24-primary-antibodies-ihc-testing-1.jpgAnti-CD24 AntibodyIHC analysis of CD24 using anti-CD24 antibody (A00373-3). CD24 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD24 Antibody (A00373-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00373-3-cd24-primary-antibodies-ihc-testing-2.jpgAnti-CD24 AntibodyIHC analysis of CD24 using anti-CD24 antibody (A00373-3). CD24 was detected in a paraffin-embedded section of human teratoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD24 Antibody (A00373-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tph2-rabbit-monoclonal-antibody-m01643-boster.html2026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01643-tph2-primary-antibodies-wb-testing-1.jpgAnti-TPH2 Rabbit Monoclonal AntibodyWestern blot analysis of TPH2 using anti-TPH2 antibody (M01643). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPH2 antigen affinity purified monoclonal antibody (M01643) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TPH2 at approximately 56 kDa. The expected band size for TPH2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01643-tph2-primary-antibodies-ihc-testing-1.jpgAnti-TPH2 Rabbit Monoclonal AntibodyIHC analysis of TPH2 using anti-TPH2 antibody (M01643). TPH2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TPH2 Antibody (M01643) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01643-tph2-primary-antibodies-ihc-testing-2.jpgAnti-TPH2 Rabbit Monoclonal AntibodyIHC analysis of TPH2 using anti-TPH2 antibody (M01643). TPH2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TPH2 Antibody (M01643) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ki67-mki67-rabbit-monoclonal-antibody-m00254-11-boster.html2026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00254-11-ki67-primary-antibodies-ihc-testing-1.jpgAnti-Ki67/MKI67 Rabbit Monoclonal AntibodyIHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-11). Ki67/MKI67 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Ki67/MKI67 Antibody (M00254-11) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00254-11-fig-3-1x.jpgAnti-Ki67/MKI67 Rabbit Monoclonal AntibodyHistological morphological features. (A) H&E staining for pathological changes of rats’ prostate tissues (left panel), and the prostate thickness of rats (right panel). (B) The distribution and expression of Ki67 in rat prostate tissues (left panel), and the proportion of Ki67 positive cells positive (%) (right panel). Red arrow: prostatic epithelium; Blue arrow: prostatic stroma. Each bar in the graph represents the mean ± S.D. Scale bar = 50 µm, n = 3. * p < 0.05, ** p < 0.01, ns, not significant; when compared with the Con group. Download full-size image DOI: Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/20173/'>41112764</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00254-11-ki67-primary-antibodies-ihc-testing-2.jpgAnti-Ki67/MKI67 Rabbit Monoclonal AntibodyIHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-11). Ki67/MKI67 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Ki67/MKI67 Antibody (M00254-11) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00254-11-ki67-primary-antibodies-ihc-testing-3.jpgAnti-Ki67/MKI67 Rabbit Monoclonal AntibodyIHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-11). Ki67/MKI67 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Ki67/MKI67 Antibody (M00254-11) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00254-11-ki67-primary-antibodies-if-testing-1.jpgAnti-Ki67/MKI67 Rabbit Monoclonal AntibodyIF analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-11). Ki67/MKI67 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated at 1:50 rabbit anti-Ki67/MKI67 Antibody (M00254-11) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00254-11-ki67-primary-antibodies-if-testing-2.jpgAnti-Ki67/MKI67 Rabbit Monoclonal AntibodyIF analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody (M00254-11). Ki67/MKI67 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 rabbit anti-Ki67/MKI67 Antibody (M00254-11) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1317482026-03-10T04:42:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1461.jpgHuman CXCL2/GRO beta ELISA Kit PicoKine®Human CXCL2/GRO beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317492026-03-10T04:42:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2294.pngRat IL-17A ELISA Kit PicoKine®Rat IL-17A PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317502026-03-10T04:42:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0666.jpgHuman BMP-6 ELISA Kit PicoKine®Human BMP-6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317512026-03-10T04:42:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0687.jpgHuman CCL26/Eotaxin-3 ELISA Kit PicoKine®Human CCL26/Eotaxin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317522026-03-10T04:42:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2112.jpgHuman CD38 ELISA Kit PicoKine®Human CD38 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317532026-03-10T04:42:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1229.jpgHuman Chymase ELISA Kit PicoKine®Human Chymase PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317542026-03-10T04:42:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2296.jpgHuman IL13RA2 ELISA Kit PicoKine®Human IL13RA2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317552026-03-10T04:42:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2297.jpgMouse IL13RA2 ELISA Kit PicoKine®Mouse IL13RA2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317562026-03-10T04:42:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2298.jpgHuman CD8 alpha ELISA Kit PicoKine®Human CD8 alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317572026-03-10T04:42:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2299.jpgHuman CD83 ELISA Kit PicoKine®Human CD83 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317582026-03-10T04:42:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2300.jpgHuman CD96v2 ELISA Kit PicoKine®Human CD96v2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317592026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2301.jpgHuman CDX2 ELISA Kit PicoKine®Human CDX2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317602026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2302.jpgHuman CEACAM-6 ELISA Kit PicoKine®Human CEACAM-6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317612026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2304.jpgHuman CTRP3/CORS26 ELISA Kit PicoKine®Human CTRP3/CORS26 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317622026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2305.jpgMouse CXCL15 ELISA Kit PicoKine®Mouse CXCL15 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317632026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2306.jpgHuman Cochlin ELISA Kit PicoKine®Human Cochlin PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317642026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2307.jpgHuman COL2A1 ELISA Kit PicoKine®Human COL2A1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317652026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2308.jpgHuman IL-2R gamma ELISA Kit PicoKine®Human IL-2R gamma PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317662026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2309.jpgHuman EDA2R/TNFRSF27/XEDAR ELISA Kit PicoKine®Human EDA2R/TNFRSF27/XEDAR PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317672026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2310.jpgHuman GASP-1 ELISA Kit PicoKine®Human GASP-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317682026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2311.jpgHuman GIT1 ELISA Kit PicoKine®Human GIT1PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317692026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2312.jpgHuman Glypican 4 ELISA Kit PicoKine®Human Glypican 4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317702026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2313.jpgHuman Hepsin ELISA Kit PicoKine®Human Hepsin PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317712026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2314.jpgHuman Hyaluronan ELISA Kit PicoKine®Human Hyaluronan PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317722026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2315.jpgHuman Siglec-9 ELISA Kit PicoKine®Human Siglec-9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317732026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2316.jpgHuman Siglec-2/CD22 ELISA Kit PicoKine®Human Siglec-2/CD22 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317742026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2317.jpgHuman Siglec-6/CD327 ELISA Kit PicoKine®Human Siglec-6/CD327 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317752026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2318.jpgMouse Hip ELISA Kit PicoKine®Mouse Hip PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317762026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2319.jpgHuman KLK15 ELISA Kit PicoKine®Human KLK15 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317772026-03-10T04:42:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2320.jpgHuman LAG-3 ELISA Kit PicoKine®Human LAG-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317782026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2321.jpgHuman LAIR2 ELISA Kit PicoKine®Human LAIR2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317792026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2323.jpgHuman MBP ELISA Kit PicoKine®Human MBP PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317802026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2324.jpgHuman MyBPC3 ELISA Kit PicoKine®Human MyBPC3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317812026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2325.jpgHuman Neuropeptide Y/NPY ELISA Kit PicoKine®Human Neuropeptide Y/NPY PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317822026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2326.jpgHuman Plasminogen ELISA Kit PicoKine®Human Plasminogen PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317832026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2327.jpgHuman RGM-A ELISA Kit PicoKine®Human RGM-A PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317842026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2328.jpgHuman VSIG8 ELISA Kit PicoKine®Human VSIG8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317852026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2329.jpgHuman VSIG1 ELISA Kit PicoKine®Human VSIG1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317862026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2330.jpgMouse/Rat RGM-A ELISA Kit PicoKine®Mouse/Rat RGM-A PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317872026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2331.jpgCanine CCL2/MCP-1 ELISA Kit PicoKine®Canine CCL2/MCP-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317882026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2332.jpgCanine IL-8 ELISA Kit PicoKine®Canine IL-8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317892026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2333.jpgCanine TNF-alpha ELISA Kit PicoKine®Canine TNF-alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317902026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2334.jpgCanine VEGF ELISA Kit PicoKine®Canine VEGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317912026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2335.jpgCanine IL-10 ELISA Kit PicoKine®Canine IL-10 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317922026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2336.jpgCanine IL-6 ELISA Kit PicoKine®Canine IL-6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317932026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2337.jpgCanine IL-4 ELISA Kit PicoKine®Canine IL-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317942026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2338.jpgCanine IL-1 beta ELISA Kit PicoKine®Canine IL-1 beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317952026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2340.jpgCanine IL-12/IL-23 p40 ELISA Kit PicoKine®Canine IL-12/IL-23 p40 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317962026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2341.jpgCanine IL-2 ELISA Kit PicoKine®Canine IL-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317972026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2342.jpgCanine GM-CSF ELISA Kit PicoKine®Canine GM-CSF PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317982026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2343.jpgCanine HGFR ELISA Kit PicoKine®Canine HGFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1317992026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2344.jpgCanine CCL5/RANTES ELISA Kit PicoKine®Canine CCL5/RANTES PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318002026-03-10T04:42:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2345.jpgCanine IFN-gamma ELISA Kit PicoKine®Canine IFN-gamma PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318012026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2346.jpgPorcine TNF-alpha ELISA Kit PicoKine®Porcine TNF-alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318022026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2347.jpgPorcine IL-6 ELISA Kit PicoKine®Porcine IL-6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318032026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2348.jpgPorcine IL-1 beta ELISA Kit PicoKine®Porcine IL-1 beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318042026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2349.jpgPorcine IL-8 ELISA Kit PicoKine®Porcine IL-8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318052026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2350.jpgPorcine IL-10 ELISA Kit PicoKine®Porcine IL-10 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318062026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2351.jpgPorcine IFN-gamma ELISA Kit PicoKine®Porcine IFN-gamma PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318072026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2352.jpgPorcine IL-4 ELISA Kit PicoKine®Porcine IFN-gamma PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318082026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2353.jpgPorcine IL-12/IL-23 p40 ELISA Kit PicoKine®Porcine IL-12/IL-23 p40 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318092026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2355.jpgPorcine IL-2 ELISA Kit PicoKine®Porcine IL-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318102026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2356.jpgPorcine IL-1 alpha ELISA Kit PicoKine®Porcine IL-1 alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318112026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2357.jpgPorcine GM-CSF ELISA Kit PicoKine®Porcine GM-CSF PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318122026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2358.jpgPorcine IL-1ra ELISA Kit PicoKine®Porcine IL-1ra PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318132026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2359.jpgEquine TNF-alpha ELISA Kit PicoKine®Equine TNF-alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318142026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2360.jpgEquine IL-1ra ELISA Kit PicoKine®Equine IL-1ra PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318152026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2361.jpgEquine IL-10 ELISA Kit PicoKine®Equine IL-10 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318162026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2362.jpgEquine IL-6 ELISA Kit PicoKine®Equine IL-6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318172026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2363.jpgEquine IFN-gamma ELISA Kit PicoKine®Equine IFN-gamma PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318182026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2364.jpgEquine IL-1 beta ELISA Kit PicoKine®Equine IL-1 beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318192026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2365.jpgEquine IL-4 ELISA Kit PicoKine®Equine IL-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318202026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2366.jpgEquine IL-2 ELISA Kit PicoKine®Equine IL-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318212026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2367.jpgFeline IFN-gamma ELISA Kit PicoKine®Feline IFN-gamma PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318222026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2368.jpgFeline IL-6 ELISA Kit PicoKine®Feline IL-6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318232026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2369.jpgFeline TNF-alpha ELISA Kit PicoKine®Feline TNF-alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318242026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2370.jpgFeline IL-10 ELISA Kit PicoKine®Feline IL-10 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318252026-03-10T04:42:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2371.jpgFeline IL-1 beta ELISA Kit PicoKine®Feline IL-1 beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318262026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2372.jpgFeline IL-8 ELISA Kit PicoKine®Feline IL-8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318272026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2373.jpgFeline IL-4 ELISA Kit PicoKine®Feline IL-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318282026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2374.jpgFeline IL-2 ELISA Kit PicoKine®Feline IL-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318292026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2375.jpgPrimate IFN-gamma ELISA Kit PicoKine®Primate IFN-gamma PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318302026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2376.jpgPrimate TNF-alpha ELISA Kit PicoKine®Primate TNF-alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318312026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2377.jpgMonkey IL-1 beta ELISA Kit PicoKine®Monkey IL-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318322026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2378.jpgMonkey IL-18 ELISA Kit PicoKine®Monkey IL-18 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318332026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2379.jpgMonkey IL-4 ELISA Kit PicoKine®Monkey IL-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318342026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2380.jpgMonkey PD-L1 ELISA Kit PicoKine®Monkey PD-L1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318352026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2382.jpgHuman Total FGFR3 ELISA Kit PicoKine®Human Total FGFR3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318362026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2383.jpgHuman Total DDR1 ELISA Kit PicoKine®Human Total DDR1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318372026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2384.jpgHuman Total DDR2 ELISA Kit PicoKine®Human Total DDR2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318382026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2385.jpgHuman Total Bcl-2 ELISA Kit PicoKine®Human Total Bcl-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318392026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2387.jpgCanine IL-17A ELISA Kit PicoKine®Canine IL-17A PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318442026-03-10T04:42:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/z/ez0845.jpgMouse TREM-1 EZ-Set™ ELISA Kit (DIY Antibody Pairs)Mouse TREM-1 EZ-Set ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1318452026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05045-1-tcirg1-primary-antibodies-ihc-testing-1.jpgAnti-TCIRG1 AntibodyIHC analysis of TCIRG1 using anti-TCIRG1 antibody (A05045-1). TCIRG1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCIRG1 Antibody (A05045-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05045-1-tcirg1-primary-antibodies-ihc-testing-2.jpgAnti-TCIRG1 AntibodyIHC analysis of TCIRG1 using anti-TCIRG1 antibody (A05045-1). TCIRG1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCIRG1 Antibody (A05045-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05045-1-tcirg1-primary-antibodies-fcm-testing-1.jpgAnti-TCIRG1 AntibodyFlow Cytometry analysis of THP-1 cells using anti-TCIRG1 antibody (A05045-1). Overlay histogram showing THP-1 cells stained with A05045-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TCIRG1 Antibody (A05045-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318462026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11411-tdrd9-primary-antibodies-wb-testing-1.jpgAnti-TDRD9 Antibody Picoband®Western blot analysis of TDRD9 using anti-TDRD9 antibody (A11411). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDRD9 antigen affinity purified polyclonal antibody (A11411) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TDRD9 at approximately 156 kDa. The expected band size for TDRD9 is at 156 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11411-tdrd9-primary-antibodies-fcm-testing-1.jpgAnti-TDRD9 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-TDRD9 antibody (A11411). Overlay histogram showing RT4 cells stained with A11411 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TDRD9 Antibody (A11411, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318472026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02390-1-sh2b1-primary-antibodies-wb-testing-1.jpgAnti-SH2B1 Antibody Picoband®Western blot analysis of SH2B1 using anti-SH2B1 antibody (A02390-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH2B1 antigen affinity purified polyclonal antibody (A02390-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SH2B1 at approximately 80-90 kDa. The expected band size for SH2B1 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02390-1-sh2b1-primary-antibodies-fcm-testing-1.jpgAnti-SH2B1 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-SH2B1 antibody (A02390-1). Overlay histogram showing SH-SY5Y cells stained with A02390-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SH2B1 Antibody (A02390-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318482026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16669-1-slfn12-primary-antibodies-wb-testing-1.jpgAnti-SLFN12 Antibody Picoband®Western blot analysis of SLFN12 using anti-SLFN12 antibody (A16669-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLFN12 antigen affinity purified polyclonal antibody (A16669-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLFN12 at approximately 67 kDa. The expected band size for SLFN12 is at 67 kDa. https://www.bosterbio.com/catalog/product/view/id/1318492026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07134-1-smyd1-primary-antibodies-wb-testing-1.jpgAnti-SMYD1 Antibody Picoband®Western blot analysis of SMYD1 using anti-SMYD1 antibody (A07134-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMYD1 antigen affinity purified polyclonal antibody (A07134-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMYD1 at approximately 57 kDa. The expected band size for SMYD1 is at 57 kDa. https://www.bosterbio.com/catalog/product/view/id/1318502026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15461-1-smyd5-primary-antibodies-wb-testing-1.jpgAnti-SMYD5 Antibody Picoband®Western blot analysis of SMYD5 using anti-SMYD5 antibody (A15461-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMYD5 antigen affinity purified polyclonal antibody (A15461-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMYD5 at approximately 47 kDa. The expected band size for SMYD5 is at 47 kDa. https://www.bosterbio.com/catalog/product/view/id/1318512026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02231-2-tdp1-primary-antibodies-wb-testing-1.jpgAnti-TDP1 Antibody Picoband®Western blot analysis of TDP1 using anti-TDP1 antibody (A02231-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDP1 antigen affinity purified polyclonal antibody (A02231-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TDP1 at approximately 70 kDa. The expected band size for TDP1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02231-2-tdp1-primary-antibodies-ihc-testing-1.jpgAnti-TDP1 Antibody Picoband®IHC analysis of TDP1 using anti-TDP1 antibody (A02231-2). TDP1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TDP1 Antibody (A02231-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02231-2-tdp1-primary-antibodies-ihc-testing-2.jpgAnti-TDP1 Antibody Picoband®IHC analysis of TDP1 using anti-TDP1 antibody (A02231-2). TDP1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TDP1 Antibody (A02231-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02231-2-tdp1-primary-antibodies-if-testing-1.jpgAnti-TDP1 Antibody Picoband®IF analysis of TDP1 using anti-TDP1 antibody (A02231-2). TDP1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TDP1 Antibody (A02231-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02231-2-tdp1-primary-antibodies-if-testing-2.jpgAnti-TDP1 Antibody Picoband®IF analysis of TDP1 using anti-TDP1 antibody (A02231-2). TDP1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TDP1 Antibody (A02231-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02231-2-tdp1-primary-antibodies-fcm-testing-1.jpgAnti-TDP1 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-TDP1 antibody (A02231-2). Overlay histogram showing MCF-7 cells stained with A02231-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TDP1 Antibody (A02231-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318522026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09038-1-tenm1-primary-antibodies-ihc-testing-1.jpgAnti-Teneurin 1/TENM1 AntibodyIHC analysis of Teneurin 1/TENM1 using anti-Teneurin 1/TENM1 antibody (A09038-1). Teneurin 1/TENM1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Teneurin 1/TENM1 Antibody (A09038-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09038-1-tenm1-primary-antibodies-if-testing-1.jpgAnti-Teneurin 1/TENM1 AntibodyIF analysis of Teneurin 1/TENM1 using anti-Teneurin 1/TENM1 antibody (A09038-1). Teneurin 1/TENM1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Teneurin 1/TENM1 Antibody (A09038-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09038-1-tenm1-primary-antibodies-fcm-testing-1.jpgAnti-Teneurin 1/TENM1 AntibodyFlow Cytometry analysis of K562 cells using anti-Teneurin 1/TENM1 antibody (A09038-1). Overlay histogram showing K562 cells stained with A09038-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Teneurin 1/TENM1 Antibody (A09038-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318532026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12135-tenm2-primary-antibodies-ihc-testing-1.jpgAnti-TENM2 AntibodyIHC analysis of TENM2 using anti-TENM2 antibody (A12135). TENM2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TENM2 Antibody (A12135) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12135-tenm2-primary-antibodies-if-testing-1.jpgAnti-TENM2 AntibodyIF analysis of TENM2 using anti-TENM2 antibody (A12135). TENM2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TENM2 Antibody (A12135) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12135-tenm2-primary-antibodies-fcm-testing-1.jpgAnti-TENM2 AntibodyFlow Cytometry analysis of U2OS cells using anti-TENM2 antibody (A12135). Overlay histogram showing U2OS cells stained with A12135 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TENM2 Antibody (A12135, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318542026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05811-2-tigar-primary-antibodies-wb-testing-1.jpgAnti-TIGAR Antibody Picoband®Western blot analysis of TIGAR using anti-TIGAR antibody (A05811-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIGAR antigen affinity purified polyclonal antibody (A05811-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TIGAR at approximately 30 kDa. The expected band size for TIGAR is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05811-2-tigar-primary-antibodies-ihc-testing-1.jpgAnti-TIGAR Antibody Picoband®IHC analysis of TIGAR using anti-TIGAR antibody (A05811-2). TIGAR was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIGAR Antibody (A05811-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05811-2-tigar-primary-antibodies-ihc-testing-2.jpgAnti-TIGAR Antibody Picoband®IHC analysis of TIGAR using anti-TIGAR antibody (A05811-2). TIGAR was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIGAR Antibody (A05811-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05811-2-tigar-primary-antibodies-if-testing-1.jpgAnti-TIGAR Antibody Picoband®IF analysis of TIGAR using anti-TIGAR antibody (A05811-2). TIGAR was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TIGAR Antibody (A05811-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05811-2-tigar-primary-antibodies-fcm-testing-1.jpgAnti-TIGAR Antibody Picoband®Flow Cytometry analysis of A431 cells using anti-TIGAR antibody (A05811-2). Overlay histogram showing A431 cells stained with A05811-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIGAR Antibody (A05811-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318552026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00950-1-tirap-primary-antibodies-ihc-testing-1.jpgAnti-TIRAP AntibodyIHC analysis of TIRAP using anti-TIRAP antibody (A00950-1). TIRAP was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIRAP Antibody (A00950-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00950-1-tirap-primary-antibodies-ihc-testing-2.jpgAnti-TIRAP AntibodyIHC analysis of TIRAP using anti-TIRAP antibody (A00950-1). TIRAP was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIRAP Antibody (A00950-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00950-1-tirap-primary-antibodies-fcm-testing-1.jpgAnti-TIRAP AntibodyFlow Cytometry analysis of HepG2 cells using anti-TIRAP antibody (A00950-1). Overlay histogram showing HepG2 cells stained with A00950-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TIRAP Antibody (A00950-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318562026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03278-2-tmpo-primary-antibodies-wb-testing-1.jpgAnti-LAP2/TMPO Antibody Picoband®Western blot analysis of LAP2/TMPO using anti-LAP2/TMPO antibody (A03278-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAP2/TMPO antigen affinity purified polyclonal antibody (A03278-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LAP2/TMPO at approximately 39,51,75 kDa. The expected band size for LAP2/TMPO is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03278-2-tmpo-primary-antibodies-ihc-testing-1.jpgAnti-LAP2/TMPO Antibody Picoband®IHC analysis of LAP2/TMPO using anti-LAP2/TMPO antibody (A03278-2). LAP2/TMPO was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LAP2/TMPO Antibody (A03278-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03278-2-tmpo-primary-antibodies-if-testing-1.jpgAnti-LAP2/TMPO Antibody Picoband®IF analysis of LAP2/TMPO using anti-LAP2/TMPO antibody (A03278-2) and anti-Alpha Tubulin antibody (M03989-3). LAP2/TMPO was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-LAP2/TMPO Antibody (A03278-2) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03278-2-tmpo-primary-antibodies-ip-testing-1.jpgAnti-LAP2/TMPO Antibody Picoband®Immunoprecipitating LAP2/TMPO in Hela whole cell lysate. Western blot analysis of LAP2/TMPO using anti-LAP2/TMPO antibody (A03278-2). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-LAP2/TMPO antibody in Hela whole cell lysate, Lane 3: anti-LAP2/TMPO antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LAP2/TMPO antigen affinity purified polyclonal antibody (A03278-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for LAP2/TMPO at approximately 75 kDa. The expected band size for LAP2/TMPO is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03278-2-tmpo-primary-antibodies-fcm-testing-1.jpgAnti-LAP2/TMPO Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-LAP2/TMPO antibody (A03278-3). Overlay histogram showing MCF-7 cells stained with A03278-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LAP2/TMPO Antibody (A03278-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318572026-03-17T05:17:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03711-2-pag1-primary-antibodies-wb-testing-1.jpgAnti-PAG1 Antibody Picoband®Western blot analysis of PAG1 using anti-PAG1 antibody (A03711-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAG1 antigen affinity purified polyclonal antibody (A03711-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAG1 at approximately 70 kDa. The expected band size for PAG1 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03711-2-pag1-primary-antibodies-if-testing-1.jpgAnti-PAG1 Antibody Picoband®IF analysis of PAG1 using anti-PAG1 antibody (A03711-2). PAG1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PAG1 Antibody (A03711-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03711-2-pag1-primary-antibodies-fcm-testing-1.jpgAnti-PAG1 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-PAG1 antibody (A03711-2). Overlay histogram showing RT4 cells stained with A03711-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PAG1 Antibody (A03711-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318582026-03-17T05:17:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13514-tdrd5-primary-antibodies-wb-testing-1.jpgAnti-TDRD5 Antibody Picoband®Western blot analysis of TDRD5 using anti-TDRD5 antibody (A13514). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDRD5 antigen affinity purified polyclonal antibody (A13514) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TDRD5 at approximately 110-120 kDa. The expected band size for TDRD5 is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13514-tdrd5-primary-antibodies-fcm-testing-1.jpgAnti-TDRD5 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-TDRD5 antibody (A13514). Overlay histogram showing MCF-7 cells stained with A13514 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TDRD5 Antibody (A13514, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318592026-03-17T05:17:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11593-1-timm10-primary-antibodies-wb-testing-1.jpgAnti-TIM10/TIMM10 Antibody Picoband®Western blot analysis of TIM10/TIMM10 using anti-TIM10/TIMM10 antibody (A11593-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human REH whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM10/TIMM10 antigen affinity purified polyclonal antibody (A11593-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TIM10/TIMM10 at approximately 10 kDa. The expected band size for TIM10/TIMM10 is at 10 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11593-1-timm10-primary-antibodies-ihc-testing-1.jpgAnti-TIM10/TIMM10 Antibody Picoband®IHC analysis of TIM10/TIMM10 using anti-TIM10/TIMM10 antibody (A11593-1). TIM10/TIMM10 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIM10/TIMM10 Antibody (A11593-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11593-1-timm10-primary-antibodies-ihc-testing-2.jpgAnti-TIM10/TIMM10 Antibody Picoband®IHC analysis of TIM10/TIMM10 using anti-TIM10/TIMM10 antibody (A11593-1). TIM10/TIMM10 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIM10/TIMM10 Antibody (A11593-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11593-1-timm10-primary-antibodies-ihc-testing-3.jpgAnti-TIM10/TIMM10 Antibody Picoband®IHC analysis of TIM10/TIMM10 using anti-TIM10/TIMM10 antibody (A11593-1). TIM10/TIMM10 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIM10/TIMM10 Antibody (A11593-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11593-1-timm10-primary-antibodies-if-testing-1.jpgAnti-TIM10/TIMM10 Antibody Picoband®IF analysis of TIM10/TIMM10 using anti-TIM10/TIMM10 antibody (A11593-1). TIM10/TIMM10 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TIM10/TIMM10 Antibody (A11593-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11593-1-timm10-primary-antibodies-if-testing-2.jpgAnti-TIM10/TIMM10 Antibody Picoband®IF analysis of TIM10/TIMM10 using anti-TIM10/TIMM10 antibody (A11593-1) and anti-Alpha Tubulin antibody (M03989-3). TIM10/TIMM10 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TIM10/TIMM10 Antibody (A11593-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11593-1-timm10-primary-antibodies-fcm-testing-1.jpgAnti-TIM10/TIMM10 Antibody Picoband®Flow Cytometry analysis of ANA-1 cells using anti-TIM10/TIMM10 antibody (A11593-1). Overlay histogram showing ANA-1 cells stained with A11593-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIM10/TIMM10 Antibody (A11593-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11593-1-timm10-primary-antibodies-fcm-testing-2.jpgAnti-TIM10/TIMM10 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-TIM10/TIMM10 antibody (A11593-1). Overlay histogram showing PC-3 cells stained with A11593-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIM10/TIMM10 Antibody (A11593-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11593-1-timm10-primary-antibodies-fcm-testing-3.jpgAnti-TIM10/TIMM10 Antibody Picoband®Flow Cytometry analysis of PC-12 cells using anti-TIM10/TIMM10 antibody (A11593-1). Overlay histogram showing PC-12 cells stained with A11593-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIM10/TIMM10 Antibody (A11593-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318602026-03-17T05:17:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04726-2-pask-primary-antibodies-fcm-testing-1.jpgAnti-PASK AntibodyFlow Cytometry analysis of HeLa cells using anti-PASK antibody (A04726-2). Overlay histogram showing HeLa cells stained with A04726-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PASK Antibody (A04726-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318612026-03-17T05:17:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13785-1-thyn1-primary-antibodies-wb-testing-1.jpgAnti-THYN1 Antibody Picoband®Western blot analysis of THYN1 using anti-THYN1 antibody (A13785-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THYN1 antigen affinity purified polyclonal antibody (A13785-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for THYN1 at approximately 26 kDa. The expected band size for THYN1 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13785-1-thyn1-primary-antibodies-if-testing-1.jpgAnti-THYN1 Antibody Picoband®IF analysis of THYN1 using anti-THYN1 antibody (A13785-1) and anti-Alpha Tubulin antibody (M03989-3). THYN1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-THYN1 Antibody (A13785-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13785-1-thyn1-primary-antibodies-ip-testing-1.jpgAnti-THYN1 Antibody Picoband®Immunoprecipitating THYN1 in Hela whole cell lysate. Western blot analysis of THYN1 using anti-THYN1 antibody (A13785-1). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-THYN1 antibody in Hela whole cell lysate, Lane 3: anti-THYN1 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-THYN1 antigen affinity purified polyclonal antibody (A13785-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for THYN1 at approximately 26 kDa. The expected band size for THYN1 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13785-1-thyn1-primary-antibodies-fcm-testing-1.jpgAnti-THYN1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-THYN1 antibody (A13785-1). Overlay histogram showing HepG2 cells stained with A13785-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-THYN1 Antibody (A13785-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318622026-03-17T05:17:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11416-1-parp11-primary-antibodies-wb-testing-1.jpgAnti-PARP11 Antibody Picoband®Western blot analysis of PARP11 using anti-PARP11 antibody (A11416-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP11 antigen affinity purified polyclonal antibody (A11416-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PARP11 at approximately 30 kDa. The expected band size for PARP11 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11416-1-parp11-primary-antibodies-ihc-testing-1.jpgAnti-PARP11 Antibody Picoband®IHC analysis of PARP11 using anti-PARP11 antibody (A11416-1). PARP11 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARP11 Antibody (A11416-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11416-1-parp11-primary-antibodies-ihc-testing-2.jpgAnti-PARP11 Antibody Picoband®IHC analysis of PARP11 using anti-PARP11 antibody (A11416-1). PARP11 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PARP11 Antibody (A11416-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11416-1-parp11-primary-antibodies-if-testing-1.jpgAnti-PARP11 Antibody Picoband®IF analysis of PARP11 using anti-PARP11 antibody (A11416-1). PARP11 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PARP11 Antibody (A11416-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11416-1-parp11-primary-antibodies-if-testing-2.jpgAnti-PARP11 Antibody Picoband®IF analysis of PARP11 using anti-PARP11 antibody (A11416-1). PARP11 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PARP11 Antibody (A11416-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11416-1-parp11-primary-antibodies-if-testing-3.jpgAnti-PARP11 Antibody Picoband®IF analysis of PARP11 using anti-PARP11 antibody (A11416-1) and anti-Alpha Tubulin antibody (M03989-3). PARP11 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PARP11 Antibody (A11416-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11416-1-parp11-primary-antibodies-fcm-testing-1.jpgAnti-PARP11 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-PARP11 antibody (A11416-1). Overlay histogram showing HEL cells stained with A11416-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARP11 Antibody (A11416-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318632026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31759-1-cemip2-primary-antibodies-wb-testing-1.jpgAnti-CEMIP2 Antibody Picoband®Western blot analysis of CEMIP2 using anti-CEMIP2 antibody (A31759-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEMIP2 antigen affinity purified polyclonal antibody (A31759-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CEMIP2 at approximately 147 kDa. The expected band size for CEMIP2 is at 147 kDa. https://www.bosterbio.com/catalog/product/view/id/1318642026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11927-1-pcp2-primary-antibodies-wb-testing-1.jpgAnti-PCP2 Antibody Picoband®Western blot analysis of PCP2 using anti-PCP2 antibody (A11927-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCP2 antigen affinity purified polyclonal antibody (A11927-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCP2 at approximately 15 kDa. The expected band size for PCP2 is at 15 kDa. https://www.bosterbio.com/catalog/product/view/id/1318652026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05582-1-pctp-primary-antibodies-wb-testing-1.jpgAnti-PCTP Antibody Picoband®Western blot analysis of PCTP using anti-PCTP antibody (A05582-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCTP antigen affinity purified polyclonal antibody (A05582-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCTP at approximately 27 kDa. The expected band size for PCTP is at 25 kDa. https://www.bosterbio.com/catalog/product/view/id/1318662026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07442-1-tpbg-primary-antibodies-wb-testing-1.jpgAnti-TPBG Antibody Picoband®Western blot analysis of TPBG using anti-TPBG antibody (A07442-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPBG antigen affinity purified polyclonal antibody (A07442-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TPBG at approximately 72 kDa. The expected band size for TPBG is at 45 kDa. https://www.bosterbio.com/catalog/product/view/id/1318672026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05187-1-tpcn2-primary-antibodies-wb-testing-1.jpgAnti-TPCN2 Antibody Picoband®Western blot analysis of TPCN2 using anti-TPCN2 antibody (A05187-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates Lane 3: rat RH35 whole cell lysates, Lane 4: mouse thymus tissue lysates, Lane 5: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPCN2 antigen affinity purified polyclonal antibody (A05187-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TPCN2 at approximately 95 kDa. The expected band size for TPCN2 is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05187-1-tpcn2-primary-antibodies-fcm-testing-1.jpgAnti-TPCN2 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-TPCN2 antibody (A05187-1). Overlay histogram showing THP-1 cells stained with A05187-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TPCN2 Antibody (A05187-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318682026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08944-2-traf3ip1-primary-antibodies-wb-testing-1.jpgAnti-TRAF3IP1 Antibody Picoband®Western blot analysis of TRAF3IP1 using anti-TRAF3IP1 antibody (A08944-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF3IP1 antigen affinity purified polyclonal antibody (A08944-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRAF3IP1 at approximately 83 kDa. The expected band size for TRAF3IP1 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08944-2-traf3ip1-primary-antibodies-fcm-testing-1.jpgAnti-TRAF3IP1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-TRAF3IP1 antibody (A08944-2). Overlay histogram showing HepG2 cells stained with A08944-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAF3IP1 Antibody (A08944-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318692026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09702-2-top1mt-primary-antibodies-wb-testing-1.jpgAnti-TOP1MT Antibody Picoband®Western blot analysis of TOP1MT using anti-TOP1MT antibody (A09702-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat herat tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOP1MT antigen affinity purified polyclonal antibody (A09702-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TOP1MT at approximately 70 kDa. The expected band size for TOP1MT is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09702-2-top1mt-primary-antibodies-ihc-testing-1.jpgAnti-TOP1MT Antibody Picoband®IHC analysis of TOP1MT using anti-TOP1MT antibody (A09702-2). TOP1MT was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOP1MT Antibody (A09702-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09702-2-top1mt-primary-antibodies-ihc-testing-2.jpgAnti-TOP1MT Antibody Picoband®IHC analysis of TOP1MT using anti-TOP1MT antibody (A09702-2). TOP1MT was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TOP1MT Antibody (A09702-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09702-2-top1mt-primary-antibodies-fcm-testing-1.jpgAnti-TOP1MT Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-TOP1MT antibody (A09702-2). Overlay histogram showing HEL cells stained with A09702-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOP1MT Antibody (A09702-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318702026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12560-3-trim10-primary-antibodies-wb-testing-1.jpgAnti-TRIM10 Antibody Picoband®Western blot analysis of TRIM10 using anti-TRIM10 antibody (A12560-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM10 antigen affinity purified polyclonal antibody (A12560-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIM10 at approximately 55 kDa. The expected band size for TRIM10 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12560-3-trim10-primary-antibodies-ihc-testing-1.jpgAnti-TRIM10 Antibody Picoband®IHC analysis of TRIM10 using anti-TRIM10 antibody (A12560-3). TRIM10 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM10 Antibody (A12560-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12560-3-trim10-primary-antibodies-ihc-testing-2.jpgAnti-TRIM10 Antibody Picoband®IHC analysis of TRIM10 using anti-TRIM10 antibody (A12560-3). TRIM10 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM10 Antibody (A12560-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12560-3-trim10-primary-antibodies-ihc-testing-3.jpgAnti-TRIM10 Antibody Picoband®IHC analysis of TRIM10 using anti-TRIM10 antibody (A12560-3). TRIM10 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM10 Antibody (A12560-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12560-3-trim10-primary-antibodies-fcm-testing-1.jpgAnti-TRIM10 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-TRIM10 antibody (A12560-3). Overlay histogram showing HEL cells stained with A12560-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM10 Antibody (A12560-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318712026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03949-2-tpd52-primary-antibodies-wb-testing-1.jpgAnti-TPD52 Antibody Picoband®Western blot analysis of TPD52 using anti-TPD52 antibody (A03949-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCT116 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPD52 antigen affinity purified polyclonal antibody (A03949-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TPD52 at approximately 23-27 kDa. The expected band size for TPD52 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03949-2-tpd52-primary-antibodies-ihc-testing-1.jpgAnti-TPD52 Antibody Picoband®IHC analysis of TPD52 using anti-TPD52 antibody (A03949-2). TPD52 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TPD52 Antibody (A03949-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03949-2-tpd52-primary-antibodies-fcm-testing-1.jpgAnti-TPD52 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-TPD52 antibody (A03949-2). Overlay histogram showing HepG2 cells stained with A03949-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TPD52 Antibody (A03949-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318722026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02179-3-traf3ip2-primary-antibodies-wb-testing-1.jpgAnti-TRAF3IP2 Antibody Picoband®Western blot analysis of TRAF3IP2 using anti-TRAF3IP2 antibody (A02179-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF3IP2 antigen affinity purified polyclonal antibody (A02179-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRAF3IP2 at approximately 65 kDa. The expected band size for TRAF3IP2 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02179-3-traf3ip2-primary-antibodies-fcm-testing-1.jpgAnti-TRAF3IP2 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-TRAF3IP2 antibody (A02179-3). Overlay histogram showing MCF-7 cells stained with A02179-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAF3IP2 Antibody (A02179-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318732026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06504-1-trdmt1-primary-antibodies-wb-testing-1.jpgAnti-DNMT2/TRDMT1 Antibody Picoband®Western blot analysis of DNMT2/TRDMT1 using anti-DNMT2/TRDMT1 antibody (A06504-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat ovary tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse ovary tissue lysates Lane 6: mouse thymus tissue lysates, Lane 7: mouse TM4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT2/TRDMT1 antigen affinity purified polyclonal antibody (A06504-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNMT2/TRDMT1 at approximately 55 kDa. The expected band size for DNMT2/TRDMT1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06504-1-trdmt1-primary-antibodies-fcm-testing-1.jpgAnti-DNMT2/TRDMT1 Antibody Picoband®Flow Cytometry analysis of RM-1 cells using anti-DNMT2/TRDMT1 antibody (A06504-1). Overlay histogram showing RM-1 cells stained with A06504-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNMT2/TRDMT1 Antibody (A06504-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318742026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10268-3-tmeff1-primary-antibodies-wb-testing-1.jpgAnti-TMEFF1 Antibody Picoband®Western blot analysis of TMEFF1 using anti-TMEFF1 antibody (A10268-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human REH whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEFF1 antigen affinity purified polyclonal antibody (A10268-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMEFF1 at approximately 41 kDa. The expected band size for TMEFF1 is at 41 kDa. https://www.bosterbio.com/catalog/product/view/id/1318752026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10779-1-tmem9-primary-antibodies-wb-testing-1.jpgAnti-TMEM9 Antibody Picoband®Western blot analysis of TMEM9 using anti-TMEM9 antibody (A10779-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM9 antigen affinity purified polyclonal antibody (A10779-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMEM9 at approximately 30 kDa. The expected band size for TMEM9 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10779-1-tmem9-primary-antibodies-fcm-testing-1.jpgAnti-TMEM9 Antibody Picoband®Flow Cytometry analysis of C6 cells using anti-TMEM9 antibody (A10779-1). Overlay histogram showing C6 cells stained with A10779-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM9 Antibody (A10779-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10779-1-tmem9-primary-antibodies-fcm-testing-2.jpgAnti-TMEM9 Antibody Picoband®Flow Cytometry analysis of EL-4 cells using anti-TMEM9 antibody (A10779-1). Overlay histogram showing EL-4 cells stained with A10779-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM9 Antibody (A10779-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10779-1-tmem9-primary-antibodies-fcm-testing-3.jpgAnti-TMEM9 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-TMEM9 antibody (A10779-1). Overlay histogram showing HEL cells stained with A10779-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM9 Antibody (A10779-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318762026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10874-2-tnfaip2-primary-antibodies-wb-testing-1.jpgAnti-TNFAIP2 Antibody Picoband®Western blot analysis of TNFAIP2 using anti-TNFAIP2 antibody (A10874-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat spleen tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFAIP2 antigen affinity purified polyclonal antibody (A10874-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNFAIP2 at approximately 73 kDa. The expected band size for TNFAIP2 is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10874-2-tnfaip2-primary-antibodies-ihc-testing-1.jpgAnti-TNFAIP2 Antibody Picoband®IHC analysis of TNFAIP2 using anti-TNFAIP2 antibody (A10874-2). TNFAIP2 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNFAIP2 Antibody (A10874-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10874-2-tnfaip2-primary-antibodies-if-testing-1.jpgAnti-TNFAIP2 Antibody Picoband®IF analysis of TNFAIP2 using anti-TNFAIP2 antibody (A10874-2). TNFAIP2 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TNFAIP2 Antibody (A10874-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10874-2-tnfaip2-primary-antibodies-fcm-testing-1.jpgAnti-TNFAIP2 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-TNFAIP2 antibody (A10874-2). Overlay histogram showing K562 cells stained with A10874-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TNFAIP2 Antibody (A10874-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318772026-03-17T05:17:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12570-1-timm8b-primary-antibodies-wb-testing-1.jpgAnti-TIMM8B Antibody Picoband®Western blot analysis of TIMM8B using anti-TIMM8B antibody (A12570-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMM8B antigen affinity purified polyclonal antibody (A12570-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TIMM8B at approximately 13 kDa. The expected band size for TIMM8B is at 9 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12570-1-timm8b-primary-antibodies-fcm-testing-1.jpgAnti-TIMM8B Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-TIMM8B antibody (A12570-1). Overlay histogram showing RT4 cells stained with A12570-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIMM8B Antibody (A12570-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318782026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12148-1-tmed1-primary-antibodies-wb-testing-1.jpgAnti-TMED1 Antibody Picoband®Western blot analysis of TMED1 using anti-TMED1 antibody (A12148-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED1 antigen affinity purified polyclonal antibody (A12148-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMED1 at approximately 25 kDa. The expected band size for TMED1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12148-1-tmed1-primary-antibodies-ihc-testing-1.jpgAnti-TMED1 Antibody Picoband®IHC analysis of TMED1 using anti-TMED1 antibody (A12148-1). TMED1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMED1 Antibody (A12148-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12148-1-tmed1-primary-antibodies-if-testing-1.jpgAnti-TMED1 Antibody Picoband®IF analysis of TMED1 using anti-TMED1 antibody (A12148-1). TMED1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TMED1 Antibody (A12148-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12148-1-tmed1-primary-antibodies-fcm-testing-1.jpgAnti-TMED1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-TMED1 antibody (A12148-1). Overlay histogram showing HepG2 cells stained with A12148-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMED1 Antibody (A12148-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318792026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16793-1-tmed8-primary-antibodies-wb-testing-1.jpgAnti-TMED8 Antibody Picoband®Western blot analysis of TMED8 using anti-TMED8 antibody (A16793-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED8 antigen affinity purified polyclonal antibody (A16793-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMED8 at approximately 36 kDa. The expected band size for TMED8 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16793-1-tmed8-primary-antibodies-fcm-testing-1.jpgAnti-TMED8 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-TMED8 antibody (A16793-1). Overlay histogram showing THP-1 cells stained with A16793-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMED8 Antibody (A16793-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318802026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07862-1-trim13-primary-antibodies-wb-testing-1.jpgAnti-TRIM13 Antibody Picoband®Western blot analysis of TRIM13 using anti-TRIM13 antibody (A07862-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human REH whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM13 antigen affinity purified polyclonal antibody (A07862-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIM13 at approximately 42 kDa. The expected band size for TRIM13 is at 47 kDa. https://www.bosterbio.com/catalog/product/view/id/1318812026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05811-3-tigar-primary-antibodies-wb-testing-1.jpgAnti-TIGAR Antibody Picoband®Western blot analysis of TIGAR using anti-TIGAR antibody (A05811-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: rat H9C2 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIGAR antigen affinity purified polyclonal antibody (A05811-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TIGAR at approximately 30 kDa. The expected band size for TIGAR is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05811-3-tigar-primary-antibodies-ihc-testing-1.jpgAnti-TIGAR Antibody Picoband®IHC analysis of TIGAR using anti-TIGAR antibody (A05811-3). TIGAR was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIGAR Antibody (A05811-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05811-3-tigar-primary-antibodies-ihc-testing-2.jpgAnti-TIGAR Antibody Picoband®IHC analysis of TIGAR using anti-TIGAR antibody (A05811-3). TIGAR was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIGAR Antibody (A05811-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05811-3-tigar-primary-antibodies-if-testing-1.jpgAnti-TIGAR Antibody Picoband®IF analysis of TIGAR using anti-TIGAR antibody (A05811-3). TIGAR was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TIGAR Antibody (A05811-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1318822026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12141-3-trim40-primary-antibodies-wb-testing-1.jpgAnti-TRIM40 Antibody Picoband®Western blot analysis of TRIM40 using anti-TRIM40 antibody (A12141-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM40 antigen affinity purified polyclonal antibody (A12141-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIM40 at approximately 36 kDa. The expected band size for TRIM40 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12141-3-trim40-primary-antibodies-ihc-testing-1.jpgAnti-TRIM40 Antibody Picoband®IHC analysis of TRIM40 using anti-TRIM40 antibody (A12141-3). TRIM40 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM40 Antibody (A12141-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12141-3-trim40-primary-antibodies-ihc-testing-2.jpgAnti-TRIM40 Antibody Picoband®IHC analysis of TRIM40 using anti-TRIM40 antibody (A12141-3). TRIM40 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM40 Antibody (A12141-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12141-3-trim40-primary-antibodies-fcm-testing-1.jpgAnti-TRIM40 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-TRIM40 antibody (A12141-3). Overlay histogram showing HEL cells stained with A12141-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM40 Antibody (A12141-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318832026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12154-2-tkfc-primary-antibodies-wb-testing-1.jpgAnti-DAK/TKFC Antibody Picoband®Western blot analysis of DAK/TKFC using anti-DAK/TKFC antibody (A12154-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAK/TKFC antigen affinity purified polyclonal antibody (A12154-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DAK/TKFC at approximately 59 kDa. The expected band size for DAK/TKFC is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12154-2-tkfc-primary-antibodies-ihc-testing-1.jpgAnti-DAK/TKFC Antibody Picoband®IHC analysis of DAK/TKFC using anti-DAK/TKFC antibody (A12154-2). DAK/TKFC was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAK/TKFC Antibody (A12154-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12154-2-tkfc-primary-antibodies-ihc-testing-2.jpgAnti-DAK/TKFC Antibody Picoband®IHC analysis of DAK/TKFC using anti-DAK/TKFC antibody (A12154-2). DAK/TKFC was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAK/TKFC Antibody (A12154-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12154-2-tkfc-primary-antibodies-ihc-testing-3.jpgAnti-DAK/TKFC Antibody Picoband®IHC analysis of DAK/TKFC using anti-DAK/TKFC antibody (A12154-2). DAK/TKFC was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAK/TKFC Antibody (A12154-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12154-2-tkfc-primary-antibodies-if-testing-1.jpgAnti-DAK/TKFC Antibody Picoband®IF analysis of DAK/TKFC using anti-DAK/TKFC antibody (A12154-2). DAK/TKFC was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DAK/TKFC Antibody (A12154-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12154-2-tkfc-primary-antibodies-fcm-testing-1.jpgAnti-DAK/TKFC Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-DAK/TKFC antibody (A12154-2). Overlay histogram showing HepG2 cells stained with A12154-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAK/TKFC Antibody (A12154-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318842026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07322-1-tmc6-primary-antibodies-wb-testing-1.jpgAnti-TMC6 Antibody Picoband®Western blot analysis of TMC6 using anti-TMC6 antibody (A07322-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMC6 antigen affinity purified polyclonal antibody (A07322-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMC6 at approximately 100 kDa. The expected band size for TMC6 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07322-1-tmc6-primary-antibodies-ihc-testing-1.jpgAnti-TMC6 Antibody Picoband®IHC analysis of TMC6 using anti-TMC6 antibody (A07322-1). TMC6 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMC6 Antibody (A07322-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07322-1-tmc6-primary-antibodies-ihc-testing-2.jpgAnti-TMC6 Antibody Picoband®IHC analysis of TMC6 using anti-TMC6 antibody (A07322-1). TMC6 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMC6 Antibody (A07322-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07322-1-tmc6-primary-antibodies-if-testing-1.jpgAnti-TMC6 Antibody Picoband®IF analysis of TMC6 using anti-TMC6 antibody (A07322-1). TMC6 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TMC6 Antibody (A07322-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07322-1-tmc6-primary-antibodies-if-testing-2.jpgAnti-TMC6 Antibody Picoband®IF analysis of TMC6 using anti-TMC6 antibody (A07322-1). TMC6 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TMC6 Antibody (A07322-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07322-1-tmc6-primary-antibodies-if-testing-3.jpgAnti-TMC6 Antibody Picoband®IF analysis of TMC6 using anti-TMC6 antibody (A07322-1) and anti-Alpha Tubulin antibody (M03989-3). TMC6 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TMC6 Antibody (A07322-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07322-1-tmc6-primary-antibodies-fcm-testing-1.jpgAnti-TMC6 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-TMC6 antibody (A07322-1). Overlay histogram showing RT4 cells stained with A07322-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMC6 Antibody (A07322-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07322-1-tmc6-primary-antibodies-fcm-testing-2.jpgAnti-TMC6 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-TMC6 antibody (A07322-1). Overlay histogram showing THP-1 cells stained with A07322-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMC6 Antibody (A07322-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318852026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05136-2-tnfrsf10d-primary-antibodies-wb-testing-1.jpgAnti-DCR2/TNFRSF10D Antibody Picoband®Western blot analysis of DCR2/TNFRSF10D using anti-DCR2/TNFRSF10D antibody (A05136-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCR2/TNFRSF10D antigen affinity purified polyclonal antibody (A05136-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCR2/TNFRSF10D at approximately 42 kDa. The expected band size for DCR2/TNFRSF10D is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05136-2-tnfrsf10d-primary-antibodies-ihc-testing-1.jpgAnti-DCR2/TNFRSF10D Antibody Picoband®IHC analysis of DCR2/TNFRSF10D using anti-DCR2/TNFRSF10D antibody (A05136-2). DCR2/TNFRSF10D was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DCR2/TNFRSF10D Antibody (A05136-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05136-2-tnfrsf10d-primary-antibodies-ihc-testing-2.jpgAnti-DCR2/TNFRSF10D Antibody Picoband®IHC analysis of DCR2/TNFRSF10D using anti-DCR2/TNFRSF10D antibody (A05136-2). DCR2/TNFRSF10D was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DCR2/TNFRSF10D Antibody (A05136-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05136-2-tnfrsf10d-primary-antibodies-ihc-testing-3.jpgAnti-DCR2/TNFRSF10D Antibody Picoband®IHC analysis of DCR2/TNFRSF10D using anti-DCR2/TNFRSF10D antibody (A05136-2). DCR2/TNFRSF10D was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DCR2/TNFRSF10D Antibody (A05136-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05136-2-tnfrsf10d-primary-antibodies-fcm-testing-1.jpgAnti-DCR2/TNFRSF10D Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-DCR2/TNFRSF10D antibody (A05136-2). Overlay histogram showing A549 cells stained with A05136-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DCR2/TNFRSF10D Antibody (A05136-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318862026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06157-1-tnfrsf19-primary-antibodies-wb-testing-1.jpgAnti-TNFRSF19 Antibody Picoband®Western blot analysis of TNFRSF19 using anti-TNFRSF19 antibody (A06157-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF19 antigen affinity purified polyclonal antibody (A06157-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNFRSF19 at approximately 46 kDa. The expected band size for TNFRSF19 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06157-1-tnfrsf19-primary-antibodies-fcm-testing-1.jpgAnti-TNFRSF19 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-TNFRSF19 antibody (A06157-1). Overlay histogram showing HepG2 cells stained with A06157-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TNFRSF19 Antibody (A06157-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06157-1-tnfrsf19-primary-antibodies-fcm-testing-2.jpgAnti-TNFRSF19 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-TNFRSF19 antibody (A06157-1). Overlay histogram showing U251 cells stained with A06157-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TNFRSF19 Antibody (A06157-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318872026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07737-1-ube2w-primary-antibodies-wb-testing-1.jpgAnti-UBE2W Antibody Picoband®Western blot analysis of UBE2W using anti-UBE2W antibody (A07737-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2W antigen affinity purified polyclonal antibody (A07737-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBE2W at approximately 17 kDa. The expected band size for UBE2W is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07737-1-ube2w-primary-antibodies-ihc-testing-1.jpgAnti-UBE2W Antibody Picoband®IHC analysis of UBE2W using anti-UBE2W antibody (A07737-1). UBE2W was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBE2W Antibody (A07737-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07737-1-ube2w-primary-antibodies-ihc-testing-2.jpgAnti-UBE2W Antibody Picoband®IHC analysis of UBE2W using anti-UBE2W antibody (A07737-1). UBE2W was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBE2W Antibody (A07737-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07737-1-ube2w-primary-antibodies-ihc-testing-3.jpgAnti-UBE2W Antibody Picoband®IHC analysis of UBE2W using anti-UBE2W antibody (A07737-1). UBE2W was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBE2W Antibody (A07737-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07737-1-ube2w-primary-antibodies-ihc-testing-4.jpgAnti-UBE2W Antibody Picoband®IHC analysis of UBE2W using anti-UBE2W antibody (A07737-1). UBE2W was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBE2W Antibody (A07737-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1318882026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10336-1-tnip3-primary-antibodies-wb-testing-1.jpgAnti-TNIP3 Antibody Picoband®Western blot analysis of TNIP3 using anti-TNIP3 antibody (A10336-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNIP3 antigen affinity purified polyclonal antibody (A10336-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNIP3 at approximately 45 kDa. The expected band size for TNIP3 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10336-1-tnip3-primary-antibodies-if-testing-1.jpgAnti-TNIP3 Antibody Picoband®IF analysis of TNIP3 using anti-TNIP3 antibody (A10336-1). TNIP3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TNIP3 Antibody (A10336-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10336-1-tnip3-primary-antibodies-fcm-testing-1.jpgAnti-TNIP3 Antibody Picoband®Flow Cytometry analysis of CACO-2 cells using anti-TNIP3 antibody (A10336-1). Overlay histogram showing CACO-2 cells stained with A10336-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNIP3 Antibody (A10336-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10336-1-tnip3-primary-antibodies-fcm-testing-2.jpgAnti-TNIP3 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-TNIP3 antibody (A10336-1). Overlay histogram showing Jurkat cells stained with A10336-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNIP3 Antibody (A10336-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318892026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14252-1-trim58-primary-antibodies-wb-testing-1.jpgAnti-TRIM58 Antibody Picoband®Western blot analysis of TRIM58 using anti-TRIM58 antibody (A14252-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM58 antigen affinity purified polyclonal antibody (A14252-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIM58 at approximately 55 kDa. The expected band size for TRIM58 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14252-1-trim58-primary-antibodies-ihc-testing-1.jpgAnti-TRIM58 Antibody Picoband®IHC analysis of TRIM58 using anti-TRIM58 antibody (A14252-1). TRIM58 was detected in a paraffin-embedded section of human pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIM58 Antibody (A14252-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14252-1-trim58-primary-antibodies-if-testing-1.jpgAnti-TRIM58 Antibody Picoband®IF analysis of TRIM58 using anti-TRIM58 antibody (A14252-1). TRIM58 was detected in a paraffin-embedded section of human pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRIM58 Antibody (A14252-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1318902026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02777-3-tsn-primary-antibodies-wb-testing-1.jpgAnti-TSN Antibody Picoband®Western blot analysis of TSN using anti-TSN antibody (A02777-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSN antigen affinity purified polyclonal antibody (A02777-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TSN at approximately 26 kDa. The expected band size for TSN is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02777-3-tsn-primary-antibodies-ihc-testing-1.jpgAnti-TSN Antibody Picoband®IHC analysis of TSN using anti-TSN antibody (A02777-3). TSN was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TSN Antibody (A02777-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02777-3-tsn-primary-antibodies-ihc-testing-2.jpgAnti-TSN Antibody Picoband®IHC analysis of TSN using anti-TSN antibody (A02777-3). TSN was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TSN Antibody (A02777-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02777-3-tsn-primary-antibodies-ihc-testing-3.jpgAnti-TSN Antibody Picoband®IHC analysis of TSN using anti-TSN antibody (A02777-3). TSN was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TSN Antibody (A02777-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02777-3-tsn-primary-antibodies-ihc-testing-4.jpgAnti-TSN Antibody Picoband®IHC analysis of TSN using anti-TSN antibody (A02777-3). TSN was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TSN Antibody (A02777-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02777-3-tsn-primary-antibodies-ihc-testing-5.jpgAnti-TSN Antibody Picoband®IHC analysis of TSN using anti-TSN antibody (A02777-3). TSN was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TSN Antibody (A02777-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02777-3-tsn-primary-antibodies-if-testing-1.jpgAnti-TSN Antibody Picoband®IF analysis of TSN using anti-TSN antibody (A02777-3). TSN was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TSN Antibody (A02777-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02777-3-tsn-primary-antibodies-fcm-testing-1.jpgAnti-TSN Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-TSN antibody (A02777-3). Overlay histogram showing 293T cells stained with A02777-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TSN Antibody (A02777-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02777-3-tsn-primary-antibodies-fcm-testing-2.jpgAnti-TSN Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-TSN antibody (A02777-3). Overlay histogram showing Jurkat cells stained with A02777-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TSN Antibody (A02777-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318912026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07489-twsg1-primary-antibodies-wb-testing-1.jpgAnti-TWSG1 Antibody Picoband®Western blot analysis of TWSG1 using anti-TWSG1 antibody (A07489). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWSG1 antigen affinity purified polyclonal antibody (A07489) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TWSG1 at approximately 33 kDa. The expected band size for TWSG1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07489-twsg1-primary-antibodies-ihc-testing-1.jpgAnti-TWSG1 Antibody Picoband®IHC analysis of TWSG1 using anti-TWSG1 antibody (A07489). TWSG1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TWSG1 Antibody (A07489) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07489-twsg1-primary-antibodies-if-testing-1.jpgAnti-TWSG1 Antibody Picoband®IF analysis of TWSG1 using anti-TWSG1 antibody (A07489). TWSG1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TWSG1 Antibody (A07489) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07489-twsg1-primary-antibodies-fcm-testing-1.jpgAnti-TWSG1 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-TWSG1 antibody (A07489). Overlay histogram showing RT4 cells stained with A07489 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TWSG1 Antibody (A07489, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318922026-03-17T05:17:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05264-2-ube2a-2b-primary-antibodies-wb-testing-1.jpgAnti-UBE2A/2B Antibody Picoband®Western blot analysis of UBE2A/2B using anti-UBE2A/2B antibody (A05264-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2A/2B antigen affinity purified polyclonal antibody (A05264-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBE2A/2B at approximately 17 kDa. The expected band size for UBE2A/2B is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05264-2-ube2a-2b-primary-antibodies-ihc-testing-1.jpgAnti-UBE2A/2B Antibody Picoband®IHC analysis of UBE2A/2B using anti-UBE2A/2B antibody (A05264-2). UBE2A/2B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBE2A/2B Antibody (A05264-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05264-2-ube2a-2b-primary-antibodies-ihc-testing-2.jpgAnti-UBE2A/2B Antibody Picoband®IHC analysis of UBE2A/2B using anti-UBE2A/2B antibody (A05264-2). UBE2A/2B was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBE2A/2B Antibody (A05264-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05264-2-ube2a-2b-primary-antibodies-if-testing-1.jpgAnti-UBE2A/2B Antibody Picoband®IF analysis of UBE2A/2B using anti-UBE2A/2B antibody (A05264-2). UBE2A/2B was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-UBE2A/2B Antibody (A05264-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05264-2-ube2a-2b-primary-antibodies-fcm-testing-1.jpgAnti-UBE2A/2B Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-UBE2A/2B antibody (A05264-2). Overlay histogram showing HeLa cells stained with A05264-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2A/2B Antibody (A05264-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05264-2-ube2a-2b-primary-antibodies-fcm-testing-2.jpgAnti-UBE2A/2B Antibody Picoband®Flow Cytometry analysis of NIH3T3 cells using anti-UBE2A/2B antibody (A05264-2). Overlay histogram showing NIH3T3 cells stained with A05264-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2A/2B Antibody (A05264-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05264-2-ube2a-2b-primary-antibodies-fcm-testing-3.jpgAnti-UBE2A/2B Antibody Picoband®Flow Cytometry analysis of PC-12 cells using anti-UBE2A/2B antibody (A05264-2). Overlay histogram showing PC-12 cells stained with A05264-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2A/2B Antibody (A05264-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318932026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09202-2-ttbk1-primary-antibodies-wb-testing-1.jpgAnti-TTBK1 Antibody Picoband®Western blot analysis of TTBK1 using anti-TTBK1 antibody (A09202-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTBK1 antigen affinity purified polyclonal antibody (A09202-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TTBK1 at approximately 153 kDa. The expected band size for TTBK1 is at 143 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09202-2-ttbk1-primary-antibodies-fcm-testing-1.jpgAnti-TTBK1 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-TTBK1 antibody (A09202-2). Overlay histogram showing SH-SY5Y cells stained with A09202-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTBK1 Antibody (A09202-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318942026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04275-2-trip6-primary-antibodies-wb-testing-1.jpgAnti-TRIP6 Antibody Picoband®Western blot analysis of TRIP6 using anti-TRIP6 antibody (A04275-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIP6 antigen affinity purified polyclonal antibody (A04275-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIP6 at approximately 50 kDa. The expected band size for TRIP6 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04275-2-trip6-primary-antibodies-ip-testing-1.jpgAnti-TRIP6 Antibody Picoband®Immunoprecipitating TRIP6 in Hela whole cell lysate. Western blot analysis of TRIP6 using anti-TRIP6 antibody (A04275-2). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TRIP6 antibody in Hela whole cell lysate, Lane 3: anti-TRIP6 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRIP6 antigen affinity purified polyclonal antibody (A04275-2) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TRIP6 at approximately 50 kDa. The expected band size for TRIP6 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04275-2-trip6-primary-antibodies-fcm-testing-1.jpgAnti-TRIP6 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-TRIP6 antibody (A04275-2). Overlay histogram showing 293T cells stained with A04275-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIP6 Antibody (A04275-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318952026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11043-1-tsr1-primary-antibodies-wb-testing-1.jpgAnti-TSR1 Antibody Picoband®Western blot analysis of TSR1 using anti-TSR1 antibody (A11043-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSR1 antigen affinity purified polyclonal antibody (A11043-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TSR1 at approximately 100 kDa. The expected band size for TSR1 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11043-1-tsr1-primary-antibodies-if-testing-1.jpgAnti-TSR1 Antibody Picoband®IF analysis of TSR1 using anti-TSR1 antibody (A11043-1) and anti-Alpha Tubulin antibody (M03989-3). TSR1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TSR1 Antibody (A11043-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11043-1-tsr1-primary-antibodies-ip-testing-1.jpgAnti-TSR1 Antibody Picoband®Immunoprecipitating TSR1 in Hela whole cell lysate. Western blot analysis of TSR1 using anti-TSR1 antibody (A11043-1). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TSR1 antibody in Hela whole cell lysate, Lane 3: anti-TSR1 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TSR1 antigen affinity purified polyclonal antibody (A11043-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TSR1 at approximately 100 kDa. The expected band size for TSR1 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11043-1-tsr1-primary-antibodies-fcm-testing-1.jpgAnti-TSR1 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-TSR1 antibody (A11043-1). Overlay histogram showing U251 cells stained with A11043-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TSR1 Antibody (A11043-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318962026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05150-1-thrap3-primary-antibodies-wb-testing-1.jpgAnti-THRAP3 Antibody Picoband®Western blot analysis of THRAP3 using anti-THRAP3 antibody (A05150-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human LNCAP whole cell lysates, Lane 3: human REH whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THRAP3 antigen affinity purified polyclonal antibody (A05150-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for THRAP3 at approximately 150 kDa. The expected band size for THRAP3 is at 109 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05150-1-thrap3-primary-antibodies-ihc-testing-1.jpgAnti-THRAP3 Antibody Picoband®IHC analysis of THRAP3 using anti-THRAP3 antibody (A05150-1). THRAP3 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-THRAP3 Antibody (A05150-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05150-1-thrap3-primary-antibodies-ihc-testing-2.jpgAnti-THRAP3 Antibody Picoband®IHC analysis of THRAP3 using anti-THRAP3 antibody (A05150-1). THRAP3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-THRAP3 Antibody (A05150-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05150-1-thrap3-primary-antibodies-ihc-testing-3.jpgAnti-THRAP3 Antibody Picoband®IHC analysis of THRAP3 using anti-THRAP3 antibody (A05150-1). THRAP3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-THRAP3 Antibody (A05150-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05150-1-thrap3-primary-antibodies-if-testing-1.jpgAnti-THRAP3 Antibody Picoband®IF analysis of THRAP3 using anti-THRAP3 antibody (A05150-1) and anti-Alpha Tubulin antibody (M03989-3). THRAP3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-THRAP3 Antibody (A05150-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05150-1-thrap3-primary-antibodies-fcm-testing-1.jpgAnti-THRAP3 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-THRAP3 antibody (A05150-1). Overlay histogram showing HEL cells stained with A05150-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-THRAP3 Antibody (A05150-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1318972026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09637-1-ttll5-primary-antibodies-wb-testing-1.jpgAnti-TTLL5 Antibody Picoband®Western blot analysis of TTLL5 using anti-TTLL5 antibody (A09637-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL5 antigen affinity purified polyclonal antibody (A09637-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TTLL5 at approximately 105 kDa. The expected band size for TTLL5 is at 144 kDa. https://www.bosterbio.com/catalog/product/view/id/1318982026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00913-2-tyro3-primary-antibodies-wb-testing-1.jpgAnti-TYRO3 Antibody Picoband®Western blot analysis of TYRO3 using anti-TYRO3 antibody (A00913-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human REH whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYRO3 antigen affinity purified polyclonal antibody (A00913-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TYRO3 at approximately 110120 kDa. The expected band size for TYRO3 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00913-2-tyro3-primary-antibodies-ihc-testing-1.jpgAnti-TYRO3 Antibody Picoband®IHC analysis of TYRO3 using anti-TYRO3 antibody (A00913-2). TYRO3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TYRO3 Antibody (A00913-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00913-2-tyro3-primary-antibodies-ihc-testing-2.jpgAnti-TYRO3 Antibody Picoband®IHC analysis of TYRO3 using anti-TYRO3 antibody (A00913-2). TYRO3 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TYRO3 Antibody (A00913-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1318992026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12380-1-ttll1-primary-antibodies-wb-testing-1.jpgAnti-TTLL1 Antibody Picoband®Western blot analysis of TTLL1 using anti-TTLL1 antibody (A12380-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL1 antigen affinity purified polyclonal antibody (A12380-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TTLL1 at approximately 40 kDa. The expected band size for TTLL1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12380-1-ttll1-primary-antibodies-ihc-testing-1.jpgAnti-TTLL1 Antibody Picoband®IHC analysis of TTLL1 using anti-TTLL1 antibody (A12380-1). TTLL1 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL1 Antibody (A12380-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1319002026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15284-1-ttll9-primary-antibodies-wb-testing-1.jpgAnti-TTLL9 Antibody Picoband®Western blot analysis of TTLL9 using anti-TTLL9 antibody (A15284-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human REH whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL9 antigen affinity purified polyclonal antibody (A15284-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TTLL9 at approximately 51 kDa. The expected band size for TTLL9 is at 51 kDa. https://www.bosterbio.com/catalog/product/view/id/1319012026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15296-1-tusc1-primary-antibodies-wb-testing-1.jpgAnti-TUSC1 Antibody Picoband®Western blot analysis of TUSC1 using anti-TUSC1 antibody (A15296-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUSC1 antigen affinity purified polyclonal antibody (A15296-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TUSC1 at approximately 25 kDa. The expected band size for TUSC1 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15296-1-tusc1-primary-antibodies-ip-testing-1.jpgAnti-TUSC1 Antibody Picoband®Immunoprecipitating TUSC1 in Hela whole cell lysate. Western blot analysis of TUSC1 using anti-TUSC1 antibody (A15296-1). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TUSC1 antibody in Hela whole cell lysate, Lane 3: anti-TUSC1 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TUSC1 antigen affinity purified polyclonal antibody (A15296-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TUSC1 at approximately 25 kDa. The expected band size for TUSC1 is at 23 kDa. https://www.bosterbio.com/catalog/product/view/id/1319022026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32117-twnk-primary-antibodies-wb-testing-1.jpgAnti-Twinkle/TWNK Antibody Picoband®Western blot analysis of Twinkle/TWNK using anti-Twinkle/TWNK antibody (A32117). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Twinkle/TWNK antigen affinity purified polyclonal antibody (A32117) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Twinkle/TWNK at approximately 70 kDa. The expected band size for Twinkle/TWNK is at 77 kDa. https://www.bosterbio.com/catalog/product/view/id/1319032026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01959-1-tyrp1-primary-antibodies-wb-testing-1.jpgAnti-TYRP1 Antibody Picoband®Western blot analysis of TYRP1 using anti-TYRP1 antibody (A01959-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse B16 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYRP1 antigen affinity purified polyclonal antibody (A01959-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TYRP1 at approximately 80 kDa. The expected band size for TYRP1 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01959-1-tyrp1-primary-antibodies-ihc-testing-1.jpgAnti-TYRP1 Antibody Picoband®IHC analysis of TYRP1 using anti-TYRP1 antibody (A01959-1). TYRP1 was detected in a paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TYRP1 Antibody (A01959-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01959-1-tyrp1-primary-antibodies-if-testing-1.jpgAnti-TYRP1 Antibody Picoband®IF analysis of TYRP1 using anti-TYRP1 antibody (A01959-1). TYRP1 was detected in a paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TYRP1 Antibody (A01959-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01959-1-tyrp1-primary-antibodies-fcm-testing-1.jpgAnti-TYRP1 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-TYRP1 antibody (A01959-1). Overlay histogram showing MCF-7 cells stained with A01959-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TYRP1 Antibody (A01959-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1319042026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07381-2-sncb-primary-antibodies-wb-testing-1.jpgAnti-SNCB Antibody Picoband®Western blot analysis of SNCB using anti-SNCB antibody (A07381-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNCB antigen affinity purified polyclonal antibody (A07381-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNCB at approximately 18 kDa. The expected band size for SNCB is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07381-2-sncb-primary-antibodies-ihc-testing-1.jpgAnti-SNCB Antibody Picoband®IHC analysis of SNCB using anti-SNCB antibody (A07381-2). SNCB was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNCB Antibody (A07381-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07381-2-sncb-primary-antibodies-ihc-testing-2.jpgAnti-SNCB Antibody Picoband®IHC analysis of SNCB using anti-SNCB antibody (A07381-2). SNCB was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNCB Antibody (A07381-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07381-2-sncb-primary-antibodies-ihc-testing-3.jpgAnti-SNCB Antibody Picoband®IHC analysis of SNCB using anti-SNCB antibody (A07381-2). SNCB was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNCB Antibody (A07381-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07381-2-sncb-primary-antibodies-ihc-testing-4.jpgAnti-SNCB Antibody Picoband®IHC analysis of SNCB using anti-SNCB antibody (A07381-2). SNCB was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNCB Antibody (A07381-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07381-2-sncb-primary-antibodies-if-testing-1.jpgAnti-SNCB Antibody Picoband®IF analysis of SNCB using anti-SNCB antibody (A07381-2). SNCB was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNCB Antibody (A07381-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07381-2-sncb-primary-antibodies-if-testing-2.jpgAnti-SNCB Antibody Picoband®IF analysis of SNCB using anti-SNCB antibody (A07381-2). SNCB was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNCB Antibody (A07381-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07381-2-sncb-primary-antibodies-if-testing-3.jpgAnti-SNCB Antibody Picoband®IF analysis of SNCB using anti-SNCB antibody (A07381-2). SNCB was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNCB Antibody (A07381-2) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-thyroglobulin-tg-picoband-antibody-aza0a193gp06-boster.html2026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a193gp06-tg-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Thyroglobulin/TG Antibody Picoband®Western blot analysis of Thyroglobulin/TG using anti-Thyroglobulin/TG antibody (AZA0A193GP06). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thyroglobulin/TG antigen affinity purified polyclonal antibody (AZA0A193GP06) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Thyroglobulin/TG at approximately 305 kDa. The expected band size for Thyroglobulin/TG is at 299 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ceruloplasmin-cp-picoband-antibody-azq7zu12-boster.html2026-03-17T05:17:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zu12-cp-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Ceruloplasmin/CP Antibody Picoband®Western blot analysis of Ceruloplasmin/CP using anti-Ceruloplasmin/CP antibody (AZQ7ZU12). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ceruloplasmin/CP antigen affinity purified polyclonal antibody (AZQ7ZU12) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Ceruloplasmin/CP at approximately 145 kDa. The expected band size for Ceruloplasmin/CP is at 125 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nkx2-4a-picoband-antibody-azb3dg22-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb3dg22-nkx2.4a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NKX2.4A Antibody Picoband®Western blot analysis of NKX2.4A using anti-NKX2.4A antibody (AZB3DG22). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKX2.4A antigen affinity purified polyclonal antibody (AZB3DG22) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NKX2.4A at approximately 38 kDa. The expected band size for NKX2.4A is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-collagen-type-i-col1a2-picoband-antibody-azq6iqx2-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6iqx2-col1a2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Collagen Type I/COL1A2 Antibody Picoband®Western blot analysis of Collagen Type I/COL1A2 using anti-Collagen Type I/COL1A2 antibody (AZQ6IQX2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen Type I/COL1A2 antigen affinity purified polyclonal antibody (AZQ6IQX2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Collagen Type I/COL1A2 at approximately 150 kDa. The expected band size for Collagen Type I/COL1A2 is at 127 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dnd-picoband-antibody-azq7t1h5-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t1h5-dnd-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DND Antibody Picoband®Western blot analysis of DND using anti-DND antibody (AZQ7T1H5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DND antigen affinity purified polyclonal antibody (AZQ7T1H5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DND at approximately 50 kDa. The expected band size for DND is at 46 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cyclin-b1-ccnb1-picoband-antibody-azq9ib44-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9ib44-ccnb1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Cyclin B1/CCNB1 Antibody Picoband®Western blot analysis of Cyclin B1/CCNB1 using anti-Cyclin B1/CCNB1 antibody (AZQ9IB44). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin B1/CCNB1 antigen affinity purified polyclonal antibody (AZQ9IB44) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cyclin B1/CCNB1 at approximately 40 kDa. The expected band size for Cyclin B1/CCNB1 is at 44 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ptf1a-antibody-azq7zsx3-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zsx3-ptf1a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PTF1A AntibodyIHC analysis of PTF1A using anti-PTF1A antibody (AZQ7ZSX3). PTF1A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTF1A Antibody (AZQ7ZSX3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lck-picoband-antibody-azq6tpq4-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6tpq4-lck-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LCK Antibody Picoband®Western blot analysis of LCK using anti-LCK antibody (AZQ6TPQ4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCK antigen affinity purified polyclonal antibody (AZQ6TPQ4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LCK at approximately 55 kDa. The expected band size for LCK is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-acc1-acaca-picoband-antibody-aza0a8m6yz52-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yz52-acaca-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ACC1/ACACA Antibody Picoband®Western blot analysis of ACC1/ACACA using anti-ACC1/ACACA antibody (AZA0A8M6YZ52). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACC1/ACACA antigen affinity purified polyclonal antibody (AZA0A8M6YZ52) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ACC1/ACACA at approximately 269 kDa. The expected band size for ACC1/ACACA is at 269 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yz52-acaca-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish ACC1/ACACA Antibody Picoband®IHC analysis of ACC1/ACACA using anti-ACC1/ACACA antibody (AZA0A8M6YZ52). ACC1/ACACA was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACC1/ACACA Antibody (AZA0A8M6YZ52) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yz52-acaca-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ACC1/ACACA Antibody Picoband®IHC analysis of ACC1/ACACA using anti-ACC1/ACACA antibody (AZA0A8M6YZ52). ACC1/ACACA was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACC1/ACACA Antibody (AZA0A8M6YZ52) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yz52-acaca-primary-antibodies-if-testing-1.jpgAnti-Zebrafish ACC1/ACACA Antibody Picoband®IF analysis of ACC1/ACACA using anti-ACC1/ACACA antibody (AZA0A8M6YZ52). ACC1/ACACA was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ACC1/ACACA Antibody (AZA0A8M6YZ52) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fn1a-picoband-antibody-azb0s602-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0s602-fn1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FN1A Antibody Picoband®Western blot analysis of FN1A using anti-FN1A antibody (AZB0S602). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FN1A antigen affinity purified polyclonal antibody (AZB0S602) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FN1A at approximately 271 kDa. The expected band size for FN1A is at 271 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cyp1b1-picoband-antibody-azb8jkd5-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jkd5-cyp1b1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CYP1B1 Antibody Picoband®Western blot analysis of CYP1B1 using anti-CYP1B1 antibody (AZB8JKD5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1B1 antigen affinity purified polyclonal antibody (AZB8JKD5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CYP1B1 at approximately 60 kDa. The expected band size for CYP1B1 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jkd5-cyp1b1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish CYP1B1 Antibody Picoband®IHC analysis of CYP1B1 using anti-CYP1B1 antibody (AZB8JKD5). CYP1B1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP1B1 Antibody (AZB8JKD5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jkd5-cyp1b1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CYP1B1 Antibody Picoband®IHC analysis of CYP1B1 using anti-CYP1B1 antibody (AZB8JKD5). CYP1B1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP1B1 Antibody (AZB8JKD5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb8jkd5-cyp1b1-primary-antibodies-if-testing-1.jpgAnti-Zebrafish CYP1B1 Antibody Picoband®IF analysis of CYP1B1 using anti-CYP1B1 antibody (AZB8JKD5). CYP1B1 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CYP1B1 Antibody (AZB8JKD5) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dusp6-picoband-antibody-azq7t2l8-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t2l8-dusp6-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DUSP6 Antibody Picoband®Western blot analysis of DUSP6 using anti-DUSP6 antibody (AZQ7T2L8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP6 antigen affinity purified polyclonal antibody (AZQ7T2L8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DUSP6 at approximately 42 kDa. The expected band size for DUSP6 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t2l8-dusp6-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish DUSP6 Antibody Picoband®IHC analysis of DUSP6 using anti-DUSP6 antibody (AZQ7T2L8). DUSP6 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DUSP6 Antibody (AZQ7T2L8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t2l8-dusp6-primary-antibodies-if-testing-1.jpgAnti-Zebrafish DUSP6 Antibody Picoband®IF analysis of DUSP6 using anti-DUSP6 antibody (AZQ7T2L8). DUSP6 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DUSP6 Antibody (AZQ7T2L8) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-vtg2-antibody-azq1mtc4-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1mtc4-vtg2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish VTG2 AntibodyIHC analysis of VTG2 using anti-VTG2 antibody (AZQ1MTC4). VTG2 was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VTG2 Antibody (AZQ1MTC4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1mtc4-vtg2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish VTG2 AntibodyIHC analysis of VTG2 using anti-VTG2 antibody (AZQ1MTC4). VTG2 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VTG2 Antibody (AZQ1MTC4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1mtc4-vtg2-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish VTG2 AntibodyIHC analysis of VTG2 using anti-VTG2 antibody (AZQ1MTC4). VTG2 was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VTG2 Antibody (AZQ1MTC4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1mtc4-vtg2-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish VTG2 AntibodyIHC analysis of VTG2 using anti-VTG2 antibody (AZQ1MTC4). VTG2 was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VTG2 Antibody (AZQ1MTC4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hsp90a-picoband-antibody-azq90474-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90474-hsp90a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HSP90A Antibody Picoband®Western blot analysis of HSP90A using anti-HSP90A antibody (AZQ90474). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90A antigen affinity purified polyclonal antibody (AZQ90474) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HSP90A at approximately 95 kDa. The expected band size for HSP90A is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90474-hsp90a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HSP90A Antibody Picoband®IHC analysis of HSP90A using anti-HSP90A antibody (AZQ90474). HSP90A was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSP90A Antibody (AZQ90474) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90474-hsp90a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HSP90A Antibody Picoband®IHC analysis of HSP90A using anti-HSP90A antibody (AZQ90474). HSP90A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSP90A Antibody (AZQ90474) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90474-hsp90a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish HSP90A Antibody Picoband®IHC analysis of HSP90A using anti-HSP90A antibody (AZQ90474). HSP90A was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSP90A Antibody (AZQ90474) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90474-hsp90a-primary-antibodies-if-testing-1.jpgAnti-Zebrafish HSP90A Antibody Picoband®IF analysis of HSP90A using anti-HSP90A antibody (AZQ90474). HSP90A was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-HSP90A Antibody (AZQ90474) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cyp11c1-antibody-aza0a8m6yv96-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yv96-cyp11c1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish CYP11C1 AntibodyIHC analysis of CYP11C1 using anti-CYP11C1 antibody (AZA0A8M6YV96). CYP11C1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP11C1 Antibody (AZA0A8M6YV96) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gsc-picoband-antibody-azp53544-boster.html2026-03-17T05:17:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp53544-gsc-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GSC Antibody Picoband®Western blot analysis of GSC using anti-GSC antibody (AZP53544). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSC antigen affinity purified polyclonal antibody (AZP53544) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSC at approximately 25 kDa. The expected band size for GSC is at 26 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nf-kb-p65-rela-picoband-antibody-aza0a8m2bi19-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bi19-rela-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NF-κB p65/RELA Antibody Picoband®Western blot analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody (AZA0A8M2BI19). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-κB p65/RELA antigen affinity purified polyclonal antibody (AZA0A8M2BI19) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NF-κB p65/RELA at approximately 70 kDa. The expected band size for NF-κB p65/RELA is at 65 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-alpha-tubulin-tuba1a-picoband-antibody-azq6nwk7-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nwk7-tuba1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Alpha Tubulin/TUBA1A Antibody Picoband®Western blot analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody (AZQ6NWK7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Tubulin/TUBA1A antigen affinity purified polyclonal antibody (AZQ6NWK7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Alpha Tubulin/TUBA1A at approximately 50 kDa. The expected band size for Alpha Tubulin/TUBA1A is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nwk7-tuba1a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Alpha Tubulin/TUBA1A Antibody Picoband®IHC analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody (AZQ6NWK7). Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Tubulin/TUBA1A Antibody (AZQ6NWK7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nwk7-tuba1a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Alpha Tubulin/TUBA1A Antibody Picoband®IHC analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody (AZQ6NWK7). Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Tubulin/TUBA1A Antibody (AZQ6NWK7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nwk7-tuba1a-primary-antibodies-if-testing-1.jpgAnti-Zebrafish Alpha Tubulin/TUBA1A Antibody Picoband®IF analysis of Alpha Tubulin/TUBA1A using anti-Alpha Tubulin/TUBA1A antibody (AZQ6NWK7). Alpha Tubulin/TUBA1A was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Alpha Tubulin/TUBA1A Antibody (AZQ6NWK7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-beta-i-tubulin-tubb1-picoband-antibody-aze7fc32-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7fc32-tubb1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish beta I Tubulin/TUBB1 Antibody Picoband®Western blot analysis of beta I Tubulin/TUBB1 using anti-beta I Tubulin/TUBB1 antibody (AZE7FC32). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta I Tubulin/TUBB1 antigen affinity purified polyclonal antibody (AZE7FC32) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for beta I Tubulin/TUBB1 at approximately 50 kDa. The expected band size for beta I Tubulin/TUBB1 is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gnat2-picoband-antibody-azq90wx5-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90wx5-gnat2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GNAT2 Antibody Picoband®Western blot analysis of GNAT2 using anti-GNAT2 antibody (AZQ90WX5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAT2 antigen affinity purified polyclonal antibody (AZQ90WX5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GNAT2 at approximately 40 kDa. The expected band size for GNAT2 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90wx5-gnat2-primary-antibodies-if-testing-1.jpgAnti-Zebrafish GNAT2 Antibody Picoband®IF analysis of GNAT2 using anti-GNAT2 antibody (AZQ90WX5). GNAT2 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GNAT2 Antibody (AZQ90WX5) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-slc5a5-picoband-antibody-aza4ig60-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza4ig60-slc5a5-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SLC5A5 Antibody Picoband®Western blot analysis of SLC5A5 using anti-SLC5A5 antibody (AZA4IG60). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A5 antigen affinity purified polyclonal antibody (AZA4IG60) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC5A5 at approximately 65 kDa. The expected band size for SLC5A5 is at 64 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-p63-tp63-picoband-antibody-azq8jhz6-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8jhz6-tp63-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish p63/TP63 Antibody Picoband®Western blot analysis of p63/TP63 using anti-p63/TP63 antibody (AZQ8JHZ6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p63/TP63 antigen affinity purified polyclonal antibody (AZQ8JHZ6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for p63/TP63 at approximately 63 kDa. The expected band size for p63/TP63 is at 63 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-acvr1l-antibody-azo73736-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo73736-acvr1l-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish ACVR1L AntibodyIHC analysis of ACVR1L using anti-ACVR1L antibody (AZO73736). ACVR1L was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACVR1L Antibody (AZO73736) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo73736-acvr1l-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish ACVR1L AntibodyIHC analysis of ACVR1L using anti-ACVR1L antibody (AZO73736). ACVR1L was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACVR1L Antibody (AZO73736) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-vegfab-picoband-antibody-aza0a8m1p9f8-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1p9f8-vegfab-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish VEGFAB Antibody Picoband®Western blot analysis of VEGFAB using anti-VEGFAB antibody (AZA0A8M1P9F8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFAB antigen affinity purified polyclonal antibody (AZA0A8M1P9F8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VEGFAB at approximately 40 kDa. The expected band size for VEGFAB is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1p9f8-vegfab-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish VEGFAB Antibody Picoband®IHC analysis of VEGFAB using anti-VEGFAB antibody (AZA0A8M1P9F8). VEGFAB was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VEGFAB Antibody (AZA0A8M1P9F8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1p9f8-vegfab-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish VEGFAB Antibody Picoband®IHC analysis of VEGFAB using anti-VEGFAB antibody (AZA0A8M1P9F8). VEGFAB was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VEGFAB Antibody (AZA0A8M1P9F8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1p9f8-vegfab-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish VEGFAB Antibody Picoband®IHC analysis of VEGFAB using anti-VEGFAB antibody (AZA0A8M1P9F8). VEGFAB was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VEGFAB Antibody (AZA0A8M1P9F8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dlb-picoband-antibody-azo57409-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo57409-dlb-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DLB Antibody Picoband®Western blot analysis of DLB using anti-DLB antibody (AZO57409). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLB antigen affinity purified polyclonal antibody (AZO57409) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DLB at approximately 67 kDa. The expected band size for DLB is at 67 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-clocka-picoband-antibody-aza0a8m9ph45-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9ph45-clocka-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CLOCKA Antibody Picoband®Western blot analysis of CLOCKA using anti-CLOCKA antibody (AZA0A8M9PH45). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLOCKA antigen affinity purified polyclonal antibody (AZA0A8M9PH45) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CLOCKA at approximately 100 kDa. The expected band size for CLOCKA is at 100 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mxa-picoband-antibody-azq8jh68-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8jh68-mxa-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MXA Antibody Picoband®Western blot analysis of MXA using anti-MXA antibody (AZQ8JH68). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MXA antigen affinity purified polyclonal antibody (AZQ8JH68) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MXA at approximately 73 kDa. The expected band size for MXA is at 73 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-axin1-picoband-antibody-azp57094-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp57094-axin1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish AXIN1 Antibody Picoband®Western blot analysis of AXIN1 using anti-AXIN1 antibody (AZP57094). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AXIN1 antigen affinity purified polyclonal antibody (AZP57094) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AXIN1 at approximately 94 kDa. The expected band size for AXIN1 is at 94 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hbbe3-picoband-antibody-azq5blf6-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5blf6-hbbe3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HBBE3 Antibody Picoband®Western blot analysis of HBBE3 using anti-HBBE3 antibody (AZQ5BLF6). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBBE3 antigen affinity purified polyclonal antibody (AZQ5BLF6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HBBE3 at approximately 16 kDa. The expected band size for HBBE3 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5blf6-hbbe3-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HBBE3 Antibody Picoband®IHC analysis of HBBE3 using anti-HBBE3 antibody (AZQ5BLF6). HBBE3 was detected in a paraffin-embedded section of zebrafish erythrocytes tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HBBE3 Antibody (AZQ5BLF6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-igf1ra-antibody-aza0a8m6ytf7-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6ytf7-igf1ra-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish IGF1RA AntibodyIHC analysis of IGF1RA using anti-IGF1RA antibody (AZA0A8M6YTF7). IGF1RA was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF1RA Antibody (AZA0A8M6YTF7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6ytf7-igf1ra-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish IGF1RA AntibodyIHC analysis of IGF1RA using anti-IGF1RA antibody (AZA0A8M6YTF7). IGF1RA was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF1RA Antibody (AZA0A8M6YTF7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6ytf7-igf1ra-primary-antibodies-if-testing-1.jpgAnti-Zebrafish IGF1RA AntibodyIF analysis of IGF1RA using anti-IGF1RA antibody (AZA0A8M6YTF7). IGF1RA was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-IGF1RA Antibody (AZA0A8M6YTF7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cadherin-17-cdh17-picoband-antibody-azq90x63-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90x63-cdh17-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Cadherin-17/CDH17 Antibody Picoband®Western blot analysis of Cadherin-17/CDH17 using anti-Cadherin-17/CDH17 antibody (AZQ90X63). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cadherin-17/CDH17 antigen affinity purified polyclonal antibody (AZQ90X63) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cadherin-17/CDH17 at approximately 120 kDa. The expected band size for Cadherin-17/CDH17 is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90x63-cdh17-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Cadherin-17/CDH17 Antibody Picoband®IHC analysis of Cadherin-17/CDH17 using anti-Cadherin-17/CDH17 antibody (AZQ90X63). Cadherin-17/CDH17 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cadherin-17/CDH17 Antibody (AZQ90X63) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90x63-cdh17-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Cadherin-17/CDH17 Antibody Picoband®IHC analysis of Cadherin-17/CDH17 using anti-Cadherin-17/CDH17 antibody (AZQ90X63). Cadherin-17/CDH17 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cadherin-17/CDH17 Antibody (AZQ90X63) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90x63-cdh17-primary-antibodies-if-testing-1.jpgAnti-Zebrafish Cadherin-17/CDH17 Antibody Picoband®IF analysis of Cadherin-17/CDH17 using anti-Cadherin-17/CDH17 antibody (AZQ90X63). Cadherin-17/CDH17 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cadherin-17/CDH17 Antibody (AZQ90X63) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tie1-picoband-antibody-azg1k2h0-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azg1k2h0-tie1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TIE1 Antibody Picoband®Western blot analysis of TIE1 using anti-TIE1 antibody (AZG1K2H0). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIE1 antigen affinity purified polyclonal antibody (AZG1K2H0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TIE1 at approximately 21 kDa. The expected band size for TIE1 is at 21 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-foxa1-picoband-antibody-azq6p0c9-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p0c9-foxa1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FOXA1 Antibody Picoband®Western blot analysis of FOXA1 using anti-FOXA1 antibody (AZQ6P0C9). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA1 antigen affinity purified polyclonal antibody (AZQ6P0C9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FOXA1 at approximately 46 kDa. The expected band size for FOXA1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p0c9-foxa1-primary-antibodies-if-testing-1.jpgAnti-Zebrafish FOXA1 Antibody Picoband®IF analysis of FOXA1 using anti-FOXA1 antibody (AZQ6P0C9). FOXA1 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FOXA1 Antibody (AZQ6P0C9) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rpl8-picoband-antibody-azq6p0v6-boster.html2026-04-06T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p0v6-rpl8-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RPL8 Antibody Picoband®Western blot analysis of RPL8 using anti-RPL8 antibody (AZQ6P0V6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL8 antigen affinity purified polyclonal antibody (AZQ6P0V6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RPL8 at approximately 35 kDa. The expected band size for RPL8 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p0v6-rpl8-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish RPL8 Antibody Picoband®IHC analysis of RPL8 using anti-RPL8 antibody (AZQ6P0V6). RPL8 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL8 Antibody (AZQ6P0V6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p0v6-rpl8-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish RPL8 Antibody Picoband®IHC analysis of RPL8 using anti-RPL8 antibody (AZQ6P0V6). RPL8 was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL8 Antibody (AZQ6P0V6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-c-jun-jun-picoband-antibody-azq6nzt5-boster.html2026-03-17T05:17:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nzt5-jun-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish c-Jun/JUN Antibody Picoband®Western blot analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (AZQ6NZT5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Jun/JUN antigen affinity purified polyclonal antibody (AZQ6NZT5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for c-Jun/JUN at approximately 39 kDa. The expected band size for c-Jun/JUN is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nzt5-jun-primary-antibodies-if-testing-1.jpgAnti-Zebrafish c-Jun/JUN Antibody Picoband®IF analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (AZQ6NZT5). c-Jun/JUN was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-c-Jun/JUN Antibody (AZQ6NZT5) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lpla-picoband-antibody-azq6p2u2-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p2u2-lpla-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LPLA Antibody Picoband®Western blot analysis of LPLA using anti-LPLA antibody (AZQ6P2U2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPLA antigen affinity purified polyclonal antibody (AZQ6P2U2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LPLA at approximately 60 kDa. The expected band size for LPLA is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-jag1b-picoband-antibody-azq90y54-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90y54-jag1b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish JAG1B Antibody Picoband®Western blot analysis of JAG1B using anti-JAG1B antibody (AZQ90Y54). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAG1B antigen affinity purified polyclonal antibody (AZQ90Y54) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for JAG1B at approximately 155 kDa. The expected band size for JAG1B is at 133 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90y54-jag1b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish JAG1B Antibody Picoband®IHC analysis of JAG1B using anti-JAG1B antibody (AZQ90Y54). JAG1B was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-JAG1B Antibody (AZQ90Y54) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90y54-jag1b-primary-antibodies-if-testing-1.jpgAnti-Zebrafish JAG1B Antibody Picoband®IF analysis of JAG1B using anti-JAG1B antibody (AZQ90Y54). JAG1B was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-JAG1B Antibody (AZQ90Y54) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-vent-picoband-antibody-azq9dgm9-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9dgm9-vent-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish VENT Antibody Picoband®Western blot analysis of VENT using anti-VENT antibody (AZQ9DGM9). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VENT antigen affinity purified polyclonal antibody (AZQ9DGM9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VENT at approximately 19 kDa. The expected band size for VENT is at 19 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gclc-picoband-antibody-azq6nv35-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nv35-gclc-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GCLC Antibody Picoband®Western blot analysis of GCLC using anti-GCLC antibody (AZQ6NV35). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLC antigen affinity purified polyclonal antibody (AZQ6NV35) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GCLC at approximately 80 kDa. The expected band size for GCLC is at 73 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-vitamin-d-binding-protein-gc-antibody-aza0a0r4itn2-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4itn2-gc-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Vitamin D binding protein/GC AntibodyIHC analysis of Vitamin D binding protein/GC using anti-Vitamin D binding protein/GC antibody (AZA0A0R4ITN2). Vitamin D binding protein/GC was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vitamin D binding protein/GC Antibody (AZA0A0R4ITN2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4itn2-gc-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Vitamin D binding protein/GC AntibodyIHC analysis of Vitamin D binding protein/GC using anti-Vitamin D binding protein/GC antibody (AZA0A0R4ITN2). Vitamin D binding protein/GC was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vitamin D binding protein/GC Antibody (AZA0A0R4ITN2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dazl-picoband-antibody-azq9ygw7-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9ygw7-dazl-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DAZL Antibody Picoband®Western blot analysis of DAZL using anti-DAZL antibody (AZQ9YGW7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAZL antigen affinity purified polyclonal antibody (AZQ9YGW7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DAZL at approximately 35 kDa. The expected band size for DAZL is at 25 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cyp3a65-picoband-antibody-azq32lt1-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq32lt1-cyp3a65-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CYP3A65 Antibody Picoband®Western blot analysis of CYP3A65 using anti-CYP3A65 antibody (AZQ32LT1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP3A65 antigen affinity purified polyclonal antibody (AZQ32LT1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CYP3A65 at approximately 59 kDa. The expected band size for CYP3A65 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq32lt1-cyp3a65-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish CYP3A65 Antibody Picoband®IHC analysis of CYP3A65 using anti-CYP3A65 antibody (AZQ32LT1). CYP3A65 was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP3A65 Antibody (AZQ32LT1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq32lt1-cyp3a65-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CYP3A65 Antibody Picoband®IHC analysis of CYP3A65 using anti-CYP3A65 antibody (AZQ32LT1). CYP3A65 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP3A65 Antibody (AZQ32LT1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq32lt1-cyp3a65-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish CYP3A65 Antibody Picoband®IHC analysis of CYP3A65 using anti-CYP3A65 antibody (AZQ32LT1). CYP3A65 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP3A65 Antibody (AZQ32LT1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq32lt1-cyp3a65-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish CYP3A65 Antibody Picoband®IHC analysis of CYP3A65 using anti-CYP3A65 antibody (AZQ32LT1). CYP3A65 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP3A65 Antibody (AZQ32LT1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cyclin-e1-ccne1-picoband-antibody-azp47794-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp47794-ccne1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Cyclin E1/CCNE1 Antibody Picoband®Western blot analysis of Cyclin E1/CCNE1 using anti-Cyclin E1/CCNE1 antibody (AZP47794). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin E1/CCNE1 antigen affinity purified polyclonal antibody (AZP47794) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cyclin E1/CCNE1 at approximately 46 kDa. The expected band size for Cyclin E1/CCNE1 is at 46 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-emx1-picoband-antibody-azq804s6-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq804s6-emx1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish EMX1 Antibody Picoband®Western blot analysis of EMX1 using anti-EMX1 antibody (AZQ804S6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EMX1 antigen affinity purified polyclonal antibody (AZQ804S6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EMX1 at approximately 26 kDa. The expected band size for EMX1 is at 26 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-piwil1-picoband-antibody-azq8uvx0-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8uvx0-piwil1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PIWIL1 Antibody Picoband®Western blot analysis of PIWIL1 using anti-PIWIL1 antibody (AZQ8UVX0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIWIL1 antigen affinity purified polyclonal antibody (AZQ8UVX0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIWIL1 at approximately 97 kDa. The expected band size for PIWIL1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8uvx0-piwil1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PIWIL1 Antibody Picoband®IHC analysis of PIWIL1 using anti-PIWIL1 antibody (AZQ8UVX0). PIWIL1 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIWIL1 Antibody (AZQ8UVX0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8uvx0-piwil1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PIWIL1 Antibody Picoband®IHC analysis of PIWIL1 using anti-PIWIL1 antibody (AZQ8UVX0). PIWIL1 was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIWIL1 Antibody (AZQ8UVX0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8uvx0-piwil1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish PIWIL1 Antibody Picoband®IHC analysis of PIWIL1 using anti-PIWIL1 antibody (AZQ8UVX0). PIWIL1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIWIL1 Antibody (AZQ8UVX0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-zic1-picoband-antibody-azo93311-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo93311-zic1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ZIC1 Antibody Picoband®Western blot analysis of ZIC1 using anti-ZIC1 antibody (AZO93311). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZIC1 antigen affinity purified polyclonal antibody (AZO93311) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ZIC1 at approximately 45 kDa. The expected band size for ZIC1 is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rplp0-picoband-antibody-azq9pv90-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pv90-rplp0-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RPLP0 Antibody Picoband®Western blot analysis of RPLP0 using anti-RPLP0 antibody (AZQ9PV90). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPLP0 antigen affinity purified polyclonal antibody (AZQ9PV90) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RPLP0 at approximately 37 kDa. The expected band size for RPLP0 is at 27 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fabp7a-b-picoband-antibody-azq9i8n9-boster.html2026-03-17T05:17:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i8n9-fabp7a-b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FABP7A/B Antibody Picoband®Western blot analysis of FABP7A/B using anti-FABP7A/B antibody (AZQ9I8N9). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP7A/B antigen affinity purified polyclonal antibody (AZQ9I8N9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FABP7A/B at approximately 15 kDa. The expected band size for FABP7A/B is at 15 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-crocc2-picoband-antibody-aza0a8m9qg25-boster.html2026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qg25-crocc2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CROCC2 Antibody Picoband®Western blot analysis of CROCC2 using anti-CROCC2 antibody (AZA0A8M9QG25). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CROCC2 antigen affinity purified polyclonal antibody (AZA0A8M9QG25) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CROCC2 at approximately 222 kDa. The expected band size for CROCC2 is at 222 kDa. https://www.bosterbio.com/catalog/product/view/id/1319542026-03-16T05:10:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319552026-03-16T05:10:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319562026-03-16T05:10:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319572026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319582026-03-16T05:10:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319592026-03-16T05:10:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319602026-03-16T05:10:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319612026-03-16T05:10:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319622026-03-16T05:10:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319632026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07882-1-psmc3ip-primary-antibodies-wb-testing-1.jpgAnti-PSMC3IP Antibody Picoband®Western blot analysis of PSMC3IP using anti-PSMC3IP antibody (A07882-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC3IP antigen affinity purified polyclonal antibody (A07882-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMC3IP at approximately 27 kDa. The expected band size for PSMC3IP is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07882-1-psmc3ip-primary-antibodies-fcm-testing-1.jpgAnti-PSMC3IP Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-PSMC3IP antibody (A07882-1). Overlay histogram showing K562 cells stained with A07882-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMC3IP Antibody (A07882-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1319642026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09799-1-slc25a22-primary-antibodies-wb-testing-1.jpgAnti-SLC25A22 Antibody Picoband®Western blot analysis of SLC25A22 using anti-SLC25A22 antibody (A09799-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A22 antigen affinity purified polyclonal antibody (A09799-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A22 at approximately 30-34 kDa. The expected band size for SLC25A22 is at 30 kDa. https://www.bosterbio.com/catalog/product/view/id/1319652026-03-16T05:10:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319662026-03-16T05:10:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319672026-03-16T05:10:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319682026-03-16T05:10:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319692026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08776-3-rbm6-primary-antibodies-wb-testing-1.jpgAnti-RBM6 Antibody Picoband®Western blot analysis of RBM6 using anti-RBM6 antibody (A08776-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM6 antigen affinity purified polyclonal antibody (A08776-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBM6 at approximately 150 kDa. The expected band size for RBM6 is at 129 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08776-3-rbm6-primary-antibodies-if-testing-1.jpgAnti-RBM6 Antibody Picoband®IF analysis of RBM6 using anti-RBM6 antibody (A08776-3) and anti-Alpha Tubulin antibody (M03989-3). RBM6 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RBM6 Antibody (A08776-3) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08776-3-rbm6-primary-antibodies-ip-testing-1.jpgAnti-RBM6 Antibody Picoband®Immunoprecipitating RBM6 in Hela whole cell lysate. Western blot analysis of RBM6 using anti-RBM6 antibody (A08776-3). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-RBM6 antibody in Hela whole cell lysate, Lane 3: anti-RBM6 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RBM6 antigen affinity purified polyclonal antibody (A08776-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RBM6 at approximately 150 kDa. The expected band size for RBM6 is at 129 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08776-3-rbm6-primary-antibodies-fcm-testing-1.jpgAnti-RBM6 Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-RBM6 antibody (A08776-3). Overlay histogram showing HeLa cells stained with A08776-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM6 Antibody (A08776-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1319702026-03-16T05:10:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319712026-03-16T05:10:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319722026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01956-1-ftl-primary-antibodies-wb-testing-1.jpgAnti-Ferritin light chain/FTL Antibody Picoband®Western blot analysis of Ferritin light chain/FTL using anti-Ferritin light chain/FTL antibody (A01956-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ferritin light chain/FTL antigen affinity purified polyclonal antibody (A01956-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Ferritin light chain/FTL at approximately 22 kDa. The expected band size for Ferritin light chain/FTL is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01956-1-ftl-primary-antibodies-ihc-testing-1.jpgAnti-Ferritin light chain/FTL Antibody Picoband®IHC analysis of Ferritin light chain/FTL using anti-Ferritin light chain/FTL antibody (A01956-1). Ferritin light chain/FTL was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ferritin light chain/FTL Antibody (A01956-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01956-1-ftl-primary-antibodies-fcm-testing-1.jpgAnti-Ferritin light chain/FTL Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-Ferritin light chain/FTL antibody (A01956-1). Overlay histogram showing 293T cells stained with A01956-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ferritin light chain/FTL Antibody (A01956-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1319732026-03-16T05:10:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319742026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02769-1-rbm8a-primary-antibodies-wb-testing-1.jpgAnti-Y14/RBM8A Antibody Picoband®Western blot analysis of RBM8A using anti-RBM8A antibody (A02769-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MDA-MB-453 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM8A antigen affinity purified polyclonal antibody (A02769-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBM8A at approximately 21 kDa. The expected band size for RBM8A is at 20 kDa. https://www.bosterbio.com/catalog/product/view/id/1319752026-03-13T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01245-4-alb-primary-antibodies-wb-testing-1.jpgAnti-Albumin/ALB AntibodyWestern blot analysis of ALB using anti-ALB antibody (A01245-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALB antigen affinity purified polyclonal antibody (A01245-4) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALB at approximately 69 kDa. The expected band size for ALB is at 69 kDa. https://www.bosterbio.com/catalog/product/view/id/1319762026-03-16T05:10:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319772026-03-16T05:10:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319782026-03-16T05:10:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319792026-03-16T05:10:12+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319802026-03-16T05:10:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319812026-03-16T05:10:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319822026-03-16T05:10:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319832026-03-16T05:10:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319842026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08984-exoc3-primary-antibodies-wb-testing-1.jpgAnti-SEC6/EXOC3 Antibody Picoband®Western blot analysis of SEC6/EXOC3 using anti-SEC6/EXOC3 antibody (A08984). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC6/EXOC3 antigen affinity purified polyclonal antibody (A08984) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SEC6/EXOC3 at approximately 86 kDa. The expected band size for SEC6/EXOC3 is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08984-exoc3-primary-antibodies-ihc-testing-1.jpgAnti-SEC6/EXOC3 Antibody Picoband®IHC analysis of SEC6/EXOC3 using anti-SEC6/EXOC3 antibody (A08984). SEC6/EXOC3 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC6/EXOC3 Antibody (A08984) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08984-exoc3-primary-antibodies-ihc-testing-2.jpgAnti-SEC6/EXOC3 Antibody Picoband®IHC analysis of SEC6/EXOC3 using anti-SEC6/EXOC3 antibody (A08984). SEC6/EXOC3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEC6/EXOC3 Antibody (A08984) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08984-exoc3-primary-antibodies-ip-testing-1.jpgAnti-SEC6/EXOC3 Antibody Picoband®Immunoprecipitating SEC6/EXOC3 in MCF-7 whole cell lysate. Western blot analysis of SEC6/EXOC3 using anti-SEC6/EXOC3 antibody (A08984). Lane 1: MCF-7 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-SEC6/EXOC3 antibody in MCF-7 whole cell lysate, Lane 3: anti-SEC6/EXOC3 antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SEC6/EXOC3 antigen affinity purified polyclonal antibody (A08984) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SEC6/EXOC3 at approximately 86 kDa. The expected band size for SEC6/EXOC3 is at 86 kDa. https://www.bosterbio.com/catalog/product/view/id/1319852026-03-16T05:10:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319862026-03-16T05:10:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319872026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319882026-03-16T05:10:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319892026-03-16T05:10:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319902026-03-16T05:10:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319912026-03-16T05:10:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319922026-03-16T05:10:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319932026-03-16T05:10:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319942026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319952026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08732-2-slc25a11-primary-antibodies-wb-testing-1.jpgAnti-SLC25A11 Antibody Picoband®Western blot analysis of SLC25A11 using anti-SLC25A11 antibody (A08732-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A11 antigen affinity purified polyclonal antibody (A08732-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A11 at approximately 34 kDa. The expected band size for SLC25A11 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08732-2-slc25a11-primary-antibodies-ihc-testing-1.jpgAnti-SLC25A11 Antibody Picoband®IHC analysis of SLC25A11 using anti-SLC25A11 antibody (A08732-2). SLC25A11 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A11 Antibody (A08732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08732-2-slc25a11-primary-antibodies-ihc-testing-2.jpgAnti-SLC25A11 Antibody Picoband®IHC analysis of SLC25A11 using anti-SLC25A11 antibody (A08732-2). SLC25A11 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A11 Antibody (A08732-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1319962026-03-16T05:10:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319972026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319982026-03-16T05:10:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1319992026-03-16T05:10:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320002026-03-16T05:10:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320012026-03-16T05:10:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320022026-03-16T05:10:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320032026-03-16T05:10:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320042026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320052026-03-16T05:10:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320062026-03-16T05:10:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320072026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320082026-03-16T05:10:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320092026-03-16T05:10:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320102026-03-16T05:10:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320112026-03-16T05:10:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320122026-03-16T05:10:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320132026-03-16T05:10:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320142026-03-16T05:10:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320152026-03-16T05:10:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10412-2-scel-primary-antibodies-wb-testing-1.jpgAnti-SCEL AntibodyWestern blot analysis of SCEL using anti-SCEL antibody (A10412-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat skin tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCEL antigen affinity purified polyclonal antibody (A10412-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SCEL at approximately 70-78 kDa. The expected band size for SCEL is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10412-2-scel-primary-antibodies-ihc-testing-1.jpgAnti-SCEL AntibodyIHC analysis of SCEL using anti-SCEL antibody (A10412-2). SCEL was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-SCEL Antibody (A10412-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1320162026-03-16T05:10:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320172026-03-16T05:10:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320182026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08820-ddhd1-primary-antibodies-wb-testing-1.jpgAnti-DDHD1 Antibody Picoband®Western blot analysis of DDHD1 using anti-DDHD1 antibody (A08820). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDHD1 antigen affinity purified polyclonal antibody (A08820) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDHD1 at approximately 110 kDa. The expected band size for DDHD1 is at 100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08820-ddhd1-primary-antibodies-fcm-testing-1.jpgAnti-DDHD1 Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-DDHD1 antibody (A08820). Overlay histogram showing HeLa cells stained with A08820 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDHD1 Antibody (A08820, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1320192026-03-16T05:10:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320202026-03-16T05:10:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320212026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06058-3-def6-primary-antibodies-wb-testing-1.jpgAnti-DEF6 Antibody Picoband®Western blot analysis of DEF6 using anti-DEF6 antibody (A06058-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DEF6 antigen affinity purified polyclonal antibody (A06058-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DEF6 at approximately 74 kDa. The expected band size for DEF6 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06058-3-def6-primary-antibodies-if-testing-1.jpgAnti-DEF6 Antibody Picoband®IF analysis of DEF6 using anti-DEF6 antibody (A06058-3) and anti-Alpha Tubulin antibody (M03989-3). DEF6 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DEF6 Antibody (A06058-3) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06058-3-def6-primary-antibodies-ip-testing-1.jpgAnti-DEF6 Antibody Picoband®Immunoprecipitating DEF6 in K562 whole cell lysate. Western blot analysis of DEF6 using anti-DEF6 antibody (A06058-3). Lane 1: K562 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DEF6 antibody in K562 whole cell lysate, Lane 3: anti-DEF6 antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DEF6 antigen affinity purified polyclonal antibody (A06058-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DEF6 at approximately 74 kDa. The expected band size for DEF6 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06058-3-def6-primary-antibodies-fcm-testing-1.jpgAnti-DEF6 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-DEF6 antibody (A06058-3). Overlay histogram showing THP-1 cells stained with A06058-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DEF6 Antibody (A06058-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1320222026-03-16T05:10:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320232026-03-16T05:10:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320242026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320252026-03-16T05:10:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320262026-03-16T05:10:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320272026-03-16T05:10:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320282026-03-16T05:10:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320292026-03-16T05:10:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320302026-03-16T05:10:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320312026-03-13T05:05:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320322026-03-16T05:10:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320332026-03-16T05:10:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320342026-03-16T05:10:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320352026-03-16T05:10:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320362026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320372026-03-16T05:10:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320382026-03-16T05:10:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320392026-03-16T05:10:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320402026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320412026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320422026-03-16T05:10:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320432026-03-16T05:10:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320442026-03-16T05:10:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320452026-04-02T05:01:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13716-1-dnajb5-primary-antibodies-wb-testing-1.jpgAnti-DNAJB5 Antibody Picoband®Western blot analysis of DNAJB5 using anti-DNAJB5 antibody (A13716-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJB5 antigen affinity purified polyclonal antibody (A13716-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNAJB5 at approximately 38 kDa. The expected band size for DNAJB5 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13716-1-dnajb5-primary-antibodies-ihc-testing-1.jpgAnti-DNAJB5 Antibody Picoband®IHC analysis of DNAJB5 using anti-DNAJB5 antibody (A13716-1). DNAJB5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAJB5 Antibody (A13716-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13716-1-dnajb5-primary-antibodies-ihc-testing-2.jpgAnti-DNAJB5 Antibody Picoband®IHC analysis of DNAJB5 using anti-DNAJB5 antibody (A13716-1). DNAJB5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAJB5 Antibody (A13716-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13716-1-dnajb5-primary-antibodies-ihc-testing-3.jpgAnti-DNAJB5 Antibody Picoband®IHC analysis of DNAJB5 using anti-DNAJB5 antibody (A13716-1). DNAJB5 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAJB5 Antibody (A13716-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13716-1-dnajb5-primary-antibodies-ihc-testing-4.jpgAnti-DNAJB5 Antibody Picoband®IHC analysis of DNAJB5 using anti-DNAJB5 antibody (A13716-1). DNAJB5 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAJB5 Antibody (A13716-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13716-1-dnajb5-primary-antibodies-if-testing-1.jpgAnti-DNAJB5 Antibody Picoband®IF analysis of DNAJB5 using anti-DNAJB5 antibody (A13716-1) and anti-Alpha Tubulin antibody (M03989-3). DNAJB5 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DNAJB5 Antibody (A13716-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13716-1-dnajb5-primary-antibodies-fcm-testing-1.jpgAnti-DNAJB5 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-DNAJB5 antibody (A13716-1). Overlay histogram showing SH-SY5Y cells stained with A13716-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAJB5 Antibody (A13716-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1320462026-03-16T05:10:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320472026-03-16T05:10:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320482026-03-16T05:10:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320492026-03-16T05:10:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320502026-03-16T05:10:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320512026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06138-dock5-primary-antibodies-wb-testing-1.jpgAnti-DOCK5 Antibody Picoband®Western blot analysis of DOCK5 using anti-DOCK5 antibody (A06138). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOCK5 antigen affinity purified polyclonal antibody (A06138) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DOCK5 at approximately 215 kDa. The expected band size for DOCK5 is at 215 kDa. https://www.bosterbio.com/catalog/product/view/id/1320522026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09048-exosc5-primary-antibodies-wb-testing-1.jpgAnti-EXOSC5 Antibody Picoband®Western blot analysis of EXOSC5 using anti-EXOSC5 antibody (A09048). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse TM4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EXOSC5 antigen affinity purified polyclonal antibody (A09048) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EXOSC5 at approximately 27 kDa. The expected band size for EXOSC5 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09048-exosc5-primary-antibodies-ihc-testing-1.jpgAnti-EXOSC5 Antibody Picoband®IHC analysis of EXOSC5 using anti-EXOSC5 antibody (A09048). EXOSC5 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EXOSC5 Antibody (A09048) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09048-exosc5-primary-antibodies-ihc-testing-2.jpgAnti-EXOSC5 Antibody Picoband®IHC analysis of EXOSC5 using anti-EXOSC5 antibody (A09048). EXOSC5 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EXOSC5 Antibody (A09048) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09048-exosc5-primary-antibodies-if-testing-1.jpgAnti-EXOSC5 Antibody Picoband®IF analysis of EXOSC5 using anti-EXOSC5 antibody (A09048) and anti-Alpha Tubulin antibody (M03989-3). EXOSC5 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EXOSC5 Antibody (A09048) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09048-exosc5-primary-antibodies-fcm-testing-1.jpgAnti-EXOSC5 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-EXOSC5 antibody (A09048). Overlay histogram showing 293T cells stained with A09048 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EXOSC5 Antibody (A09048, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1320532026-03-16T05:10:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320542026-03-16T05:10:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320552026-03-16T05:10:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320562026-03-16T05:10:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320572026-03-16T05:10:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320582026-03-16T05:10:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320592026-03-16T05:10:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320602026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320612026-03-16T05:10:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320622026-03-16T05:10:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320632026-03-16T05:10:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320642026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320652026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320662026-03-16T05:10:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320672026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07285-1-ddhd2-primary-antibodies-wb-testing-1.jpgAnti-DDHD2 Antibody Picoband®Western blot analysis of DDHD2 using anti-DDHD2 antibody (A07285-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDHD2 antigen affinity purified polyclonal antibody (A07285-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDHD2 at approximately 90 kDa. The expected band size for DDHD2 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07285-1-ddhd2-primary-antibodies-fcm-testing-1.jpgAnti-DDHD2 Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-DDHD2 antibody (A07285-1). Overlay histogram showing HeLa cells stained with A07285-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDHD2 Antibody (A07285-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1320682026-03-16T05:10:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320692026-03-16T05:10:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320702026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07079-1-fam13a-primary-antibodies-wb-testing-1.jpgAnti-FAM13A Antibody Picoband®Western blot analysis of FAM13A using anti-FAM13A antibody (A07079-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM13A antigen affinity purified polyclonal antibody (A07079-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FAM13A at approximately 90 kDa. The expected band size for FAM13A is at 117 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07079-1-fam13a-primary-antibodies-fcm-testing-1.jpgAnti-FAM13A Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-FAM13A antibody (A07079-1). Overlay histogram showing RT4 cells stained with A07079-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FAM13A Antibody (A07079-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1320712026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12665-1-chchd1-primary-antibodies-wb-testing-1.jpgAnti-CHCHD1 Antibody Picoband®Western blot analysis of CHCHD1 using anti-CHCHD1 antibody (A12665-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHCHD1 antigen affinity purified polyclonal antibody (A12665-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CHCHD1 at approximately 13 kDa. The expected band size for CHCHD1 is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12665-1-chchd1-primary-antibodies-ihc-testing-1.jpgAnti-CHCHD1 Antibody Picoband®IHC analysis of CHCHD1 using anti-CHCHD1 antibody (A12665-1). CHCHD1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CHCHD1 Antibody (A12665-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12665-1-chchd1-primary-antibodies-ihc-testing-2.jpgAnti-CHCHD1 Antibody Picoband®IHC analysis of CHCHD1 using anti-CHCHD1 antibody (A12665-1). CHCHD1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CHCHD1 Antibody (A12665-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12665-1-chchd1-primary-antibodies-if-testing-1.jpgAnti-CHCHD1 Antibody Picoband®IF analysis of CHCHD1 using anti-CHCHD1 antibody (A12665-1) and anti-Alpha Tubulin antibody (M03989-3). CHCHD1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CHCHD1 Antibody (A12665-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1320722026-03-16T05:10:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320732026-03-16T05:10:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320742026-03-16T05:10:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320752026-03-16T05:10:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320762026-03-16T05:10:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320772026-03-16T05:10:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320782026-03-16T05:10:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320792026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320802026-03-16T05:10:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320812026-03-16T05:10:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320822026-03-16T05:10:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320832026-03-16T05:10:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320842026-03-16T05:10:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320852026-03-16T05:10:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320862026-03-16T05:10:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320872026-03-16T05:10:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320882026-03-16T05:10:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320892026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320902026-03-16T05:10:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320912026-03-16T05:10:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320922026-03-16T05:10:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320932026-03-16T05:10:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320942026-03-16T05:10:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320952026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09102-1-zdhhc13-primary-antibodies-ihc-testing-1.jpgAnti-ZDHHC13 AntibodyIHC analysis of ZDHHC13 using anti-ZDHHC13 antibody (A09102-1). ZDHHC13 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-ZDHHC13 Antibody (A09102-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09102-1-zdhhc13-primary-antibodies-ihc-testing-2.jpgAnti-ZDHHC13 AntibodyIHC analysis of ZDHHC13 using anti-ZDHHC13 antibody (A09102-1). ZDHHC13 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-ZDHHC13 Antibody (A09102-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1320962026-03-16T05:10:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320972026-03-16T05:10:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320982026-03-16T05:10:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1320992026-03-16T05:10:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321002026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321012026-03-16T05:10:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321022026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321032026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321042026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321052026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321062026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321072026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321082026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321092026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321102026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321112026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321122026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321132026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321142026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321152026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321162026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321172026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321182026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321192026-03-16T05:10:26+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321202026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321212026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321222026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321232026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321242026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321252026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321262026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321272026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321282026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321292026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321302026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321312026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321322026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321332026-04-02T05:01:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10183-1-dhx16-primary-antibodies-wb-testing-1.jpgAnti-DHX16 Antibody Picoband®Western blot analysis of DHX16 using anti-DHX16 antibody (A10183-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX16 antigen affinity purified polyclonal antibody (A10183-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHX16 at approximately 119 kDa. The expected band size for DHX16 is at 119 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10183-1-dhx16-primary-antibodies-if-testing-1.jpgAnti-DHX16 Antibody Picoband®IF analysis of DHX16 using anti-DHX16 antibody (A10183-1) and anti-Alpha Tubulin antibody (M03989-3). DHX16 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DHX16 Antibody (A10183-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1321342026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321352026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321362026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321372026-03-16T05:10:27+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321382026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321392026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321402026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321412026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321422026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321432026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321442026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321452026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321462026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321472026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321482026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321492026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321502026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321512026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321522026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321532026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321542026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321552026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321562026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321572026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321582026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321592026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321602026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321612026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321622026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321632026-03-16T05:10:28+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321642026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02806-1-slc20a2-primary-antibodies-wb-testing-1.jpgAnti-SLC20A2 Antibody Picoband®Western blot analysis of SLC20A2 using anti-SLC20A2 antibody (A02806-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC20A2 antigen affinity purified polyclonal antibody (A02806-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC20A2 at approximately 85-90 kDa. The expected band size for SLC20A2 is at 67 kDa. https://www.bosterbio.com/catalog/product/view/id/1321652026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04435-1-drg1-primary-antibodies-wb-testing-1.jpgAnti-DRG1 Antibody Picoband®Western blot analysis of DRG1 using anti-DRG1 antibody (A04435-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRG1 antigen affinity purified polyclonal antibody (A04435-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DRG1 at approximately 41 kDa. The expected band size for DRG1 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04435-1-drg1-primary-antibodies-ihc-testing-1.jpgAnti-DRG1 Antibody Picoband®IHC analysis of DRG1 using anti-DRG1 antibody (A04435-1). DRG1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DRG1 Antibody (A04435-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04435-1-drg1-primary-antibodies-ihc-testing-2.jpgAnti-DRG1 Antibody Picoband®IHC analysis of DRG1 using anti-DRG1 antibody (A04435-1). DRG1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DRG1 Antibody (A04435-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04435-1-drg1-primary-antibodies-if-testing-1.jpgAnti-DRG1 Antibody Picoband®IF analysis of DRG1 using anti-DRG1 antibody (A04435-1) and anti-Alpha Tubulin antibody (M03989-3). DRG1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DRG1 Antibody (A04435-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04435-1-drg1-primary-antibodies-fcm-testing-1.jpgAnti-DRG1 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-DRG1 antibody (A04435-1). Overlay histogram showing 293T cells stained with A04435-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DRG1 Antibody (A04435-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1321662026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321672026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321682026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321692026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321702026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321712026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321722026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321732026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321742026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321752026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321762026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321772026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321782026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321792026-04-02T05:01:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10196-1-ergic2-primary-antibodies-wb-testing-1.jpgAnti-ERGIC2 Antibody Picoband®Western blot analysis of ERGIC2 using anti-ERGIC2 antibody (A10196-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERGIC2 antigen affinity purified polyclonal antibody (A10196-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERGIC2 at approximately 45 kDa. The expected band size for ERGIC2 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10196-1-ergic2-primary-antibodies-ihc-testing-1.jpgAnti-ERGIC2 Antibody Picoband®IHC analysis of ERGIC2 using anti-ERGIC2 antibody (A10196-1). ERGIC2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ERGIC2 Antibody (A10196-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10196-1-ergic2-primary-antibodies-ihc-testing-2.jpgAnti-ERGIC2 Antibody Picoband®IHC analysis of ERGIC2 using anti-ERGIC2 antibody (A10196-1). ERGIC2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ERGIC2 Antibody (A10196-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10196-1-ergic2-primary-antibodies-if-testing-1.jpgAnti-ERGIC2 Antibody Picoband®IF analysis of ERGIC2 using anti-ERGIC2 antibody (A10196-1) and anti-Alpha Tubulin antibody (M03989-3). ERGIC2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ERGIC2 Antibody (A10196-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10196-1-ergic2-primary-antibodies-ip-testing-1.jpgAnti-ERGIC2 Antibody Picoband®Immunoprecipitating ERGIC2 in Hela whole cell lysate. Western blot analysis of ERGIC2 using anti-ERGIC2 antibody (A10196-1). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-ERGIC2 antibody in Hela whole cell lysate, Lane 3: anti-ERGIC2 antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERGIC2 antigen affinity purified polyclonal antibody (A10196-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ERGIC2 at approximately 45 kDa. The expected band size for ERGIC2 is at 43 kDa. https://www.bosterbio.com/catalog/product/view/id/1321802026-04-02T05:01:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11064-3-eps8l2-primary-antibodies-wb-testing-1.jpgAnti-EPS8L2 Antibody Picoband®Western blot analysis of EPS8L2 using anti-EPS8L2 antibody (A11064-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPS8L2 antigen affinity purified polyclonal antibody (A11064-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPS8L2 at approximately 85 kDa. The expected band size for EPS8L2 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11064-3-eps8l2-primary-antibodies-ihc-testing-1.jpgAnti-EPS8L2 Antibody Picoband®IHC analysis of EPS8L2 using anti-EPS8L2 antibody (A11064-3). EPS8L2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPS8L2 Antibody (A11064-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11064-3-eps8l2-primary-antibodies-if-testing-1.jpgAnti-EPS8L2 Antibody Picoband®IF analysis of EPS8L2 using anti-EPS8L2 antibody (A11064-3). EPS8L2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EPS8L2 Antibody (A11064-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11064-3-eps8l2-primary-antibodies-ip-testing-1.jpgAnti-EPS8L2 Antibody Picoband®Immunoprecipitating EPS8L2 in HepG2 whole cell lysate. Western blot analysis of EPS8L2 using anti-EPS8L2 antibody (A11064-3). Lane 1: HepG2 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-EPS8L2 antibody in HepG2 whole cell lysate, Lane 3: anti-EPS8L2 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EPS8L2 antigen affinity purified polyclonal antibody (A11064-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EPS8L2 at approximately 85 kDa. The expected band size for EPS8L2 is at 81 kDa. https://www.bosterbio.com/catalog/product/view/id/1321812026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321822026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321832026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321842026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321852026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321862026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321872026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321882026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07501-1-slc17a9-primary-antibodies-wb-testing-1_1.jpgAnti-SLC17A9 Antibody Picoband®Western blot analysis of SLC17A9 using anti-SLC17A9 antibody (A07501-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC17A9 antigen affinity purified polyclonal antibody (A07501-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC17A9 at approximately 70 kDa. The expected band size for SLC17A9 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07501-1-slc17a9-primary-antibodies-ihc-testing-1.jpgAnti-SLC17A9 Antibody Picoband®IHC analysis of SLC17A9 using anti-SLC17A9 antibody (A07501-1). SLC17A9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC17A9 Antibody (A07501-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07501-1-slc17a9-primary-antibodies-fcm-testing-1.jpgAnti-SLC17A9 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-SLC17A9 antibody (A07501-1). Overlay histogram showing HepG2 cells stained with A07501-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC17A9 Antibody (A07501-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1321892026-03-16T05:10:29+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321902026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321912026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321922026-03-16T05:10:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05685-1-napsa-primary-antibodies-wb-testing-1.jpgAnti-Napsin A/NAPSA AntibodyWestern blot analysis of NAPSA using anti-NAPSA antibody (A05685-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAPSA antigen affinity purified polyclonal antibody (A05685-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NAPSA at approximately 40 kDa. The expected band size for NAPSA is at 45 kDa. https://www.bosterbio.com/catalog/product/view/id/1321932026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321942026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05959-2-slc9a9-primary-antibodies-wb-testing-1.jpgAnti-SLC9A9 Antibody Picoband®Western blot analysis of SLC9A9 using anti-SLC9A9 antibody (A05959-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A9 antigen affinity purified polyclonal antibody (A05959-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC9A9 at approximately 60 kDa. The expected band size for SLC9A9 is at 73 kDa. https://www.bosterbio.com/catalog/product/view/id/1321952026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321962026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321972026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321982026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1321992026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322002026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322012026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322022026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322032026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322042026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322052026-03-13T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00559-1-fbn1-primary-antibodies-ihc-testing-1.jpgAnti-Fibrillin 1/FBN1 AntibodyIHC analysis of FBN1 using anti-FBN1 antibody (A00559-1). FBN1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-FBN1 Antibody (A00559-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00559-1-fbn1-primary-antibodies-ihc-testing-2.jpgAnti-Fibrillin 1/FBN1 AntibodyIHC analysis of FBN1 using anti-FBN1 antibody (A00559-1). FBN1 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-FBN1 Antibody (A00559-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00559-1-fbn1-primary-antibodies-ihc-testing-3.jpgAnti-Fibrillin 1/FBN1 AntibodyIHC analysis of FBN1 using anti-FBN1 antibody (A00559-1). FBN1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-FBN1 Antibody (A00559-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00559-1-fbn1-primary-antibodies-ihc-testing-4.jpgAnti-Fibrillin 1/FBN1 AntibodyIHC analysis of FBN1 using anti-FBN1 antibody (A00559-1). FBN1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-FBN1 Antibody (A00559-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00559-1-fbn1-primary-antibodies-ihc-testing-5.jpgAnti-Fibrillin 1/FBN1 AntibodyIHC analysis of FBN1 using anti-FBN1 antibody (A00559-1). FBN1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-FBN1 Antibody (A00559-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1322062026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06205-2-stom-primary-antibodies-wb-testing-1.jpgAnti-Stomatin/STOM Antibody Picoband®Western blot analysis of STOM using anti-STOM antibody (A06205-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STOM antigen affinity purified polyclonal antibody (A06205-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STOM at approximately 32 kDa. The expected band size for STOM is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06205-2-stom-primary-antibodies-fcm-testing-1.jpgAnti-Stomatin/STOM Antibody Picoband®Flow Cytometry analysis of SiHa cells using anti-STOM antibody (A06205-2). Overlay histogram showing SiHa cells stained with A06205-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-STOM Antibody (A06205-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1322072026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322082026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322092026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322102026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322112026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00392-1-rara-primary-antibodies-wb-testing-1.jpgAnti-RARA Antibody Picoband®Western blot analysis of RARA using anti-RARA antibody (A00392-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RARA antigen affinity purified polyclonal antibody (A00392-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RARA at approximately 55 kDa. The expected band size for RARA is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00392-1-rara-primary-antibodies-ihc-testing-1.jpgAnti-RARA Antibody Picoband®IHC analysis of RARA using anti-RARA antibody (A00392-1). RARA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RARA Antibody (A00392-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00392-1-rara-primary-antibodies-ihc-testing-2.jpgAnti-RARA Antibody Picoband®IHC analysis of RARA using anti-RARA antibody (A00392-1). RARA was detected in a paraffin-embedded section of human prostatic hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RARA Antibody (A00392-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00392-1-rara-primary-antibodies-ihc-testing-3.jpgAnti-RARA Antibody Picoband®IHC analysis of RARA using anti-RARA antibody (A00392-1). RARA was detected in a paraffin-embedded section of human prostatic hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RARA Antibody (A00392-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00392-1-rara-primary-antibodies-ihc-testing-4.jpgAnti-RARA Antibody Picoband®IHC analysis of RARA using anti-RARA antibody (A00392-1). RARA was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RARA Antibody (A00392-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00392-1-rara-primary-antibodies-ihc-testing-5.jpgAnti-RARA Antibody Picoband®IHC analysis of RARA using anti-RARA antibody (A00392-1). RARA was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RARA Antibody (A00392-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1322122026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322132026-03-13T05:05:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322142026-03-16T05:10:30+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322152026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322162026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322172026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322182026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322192026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322202026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322212026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322222026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322232026-04-05T05:00:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322242026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322252026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322262026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322272026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322282026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322292026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322302026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322312026-03-16T05:10:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322322026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322332026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322342026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322352026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322362026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322372026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322382026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322392026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322402026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322412026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322422026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322432026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322442026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322452026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322462026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322472026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322482026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322492026-03-16T05:10:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09457-2-slc25a6-primary-antibodies-wb-testing-1.jpgAnti-SLC25A6 AntibodyWestern blot analysis of SLC25A6 using anti-SLC25A6 antibody (A09457-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A6 antigen affinity purified polyclonal antibody (A09457-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A6 at approximately 33 kDa. The expected band size for SLC25A6 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09457-2-slc25a6-primary-antibodies-ihc-testing-1.jpgAnti-SLC25A6 AntibodyIHC analysis of SLC25A6 using anti-SLC25A6 antibody (A09457-2). SLC25A6 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-SLC25A6 Antibody (A09457-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09457-2-slc25a6-primary-antibodies-ihc-testing-2.jpgAnti-SLC25A6 AntibodyIHC analysis of SLC25A6 using anti-SLC25A6 antibody (A09457-2). SLC25A6 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-SLC25A6 Antibody (A09457-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1322502026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322512026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322522026-03-16T05:10:32+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322532026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322542026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322552026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322562026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322572026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322582026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322592026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322602026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322612026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322622026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322632026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322642026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322652026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322662026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322672026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322682026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322692026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322702026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322712026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322722026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322732026-03-16T05:10:33+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322742026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322752026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322762026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322772026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322782026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322792026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322802026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322812026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322822026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322832026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322842026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322852026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322862026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322872026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322882026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322892026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322902026-03-16T05:10:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322912026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322922026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322932026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322942026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322952026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322962026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322972026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322982026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1322992026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323002026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323012026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323022026-03-17T05:17:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08644-1-dpysl5-primary-antibodies-wb-testing-1.jpgAnti-DPYSL5 Antibody Picoband®Western blot analysis of DPYSL5 using anti-DPYSL5 antibody (A08644-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPYSL5 antigen affinity purified polyclonal antibody (A08644-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DPYSL5 at approximately 61 kDa. The expected band size for DPYSL5 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08644-1-dpysl5-primary-antibodies-ip-testing-1.jpgAnti-DPYSL5 Antibody Picoband®Immunoprecipitating DPYSL5 in SH-SY5Y whole cell lysate. Western blot analysis of DPYSL5 using anti-DPYSL5 antibody (A08644-1). Lane 1: SH-SY5Y whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DPYSL5 antibody in SH-SY5Y whole cell lysate, Lane 3: anti-DPYSL5 antibody (2μg) + SH-SY5Y whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DPYSL5 antigen affinity purified polyclonal antibody (A08644-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DPYSL5 at approximately 61 kDa. The expected band size for DPYSL5 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08644-1-dpysl5-primary-antibodies-fcm-testing-1.jpgAnti-DPYSL5 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-DPYSL5 antibody (A08644-1). Overlay histogram showing SH-SY5Y cells stained with A08644-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DPYSL5 Antibody (A08644-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1323032026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323042026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323052026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323062026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323072026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323082026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323092026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323102026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323112026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323122026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323132026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323142026-03-16T05:10:35+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323152026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323162026-04-02T05:01:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08512-1-dnase2-primary-antibodies-wb-testing-1.jpgAnti-DNASE2 Antibody Picoband®Western blot analysis of DNASE2 using anti-DNASE2 antibody (A08512-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNASE2 antigen affinity purified polyclonal antibody (A08512-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNASE2 at approximately 32 kDa. The expected band size for DNASE2 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08512-1-dnase2-primary-antibodies-ihc-testing-1.jpgAnti-DNASE2 Antibody Picoband®IHC analysis of DNASE2 using anti-DNASE2 antibody (A08512-1). DNASE2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNASE2 Antibody (A08512-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1323172026-03-16T05:10:36+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323182026-03-16T05:10:36+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323192026-03-16T05:10:36+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323202026-03-16T05:10:36+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323212026-04-02T05:01:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09768-dcbld2-primary-antibodies-wb-testing-1.jpgAnti-DCBLD2 Antibody Picoband®Western blot analysis of DCBLD2 using anti-DCBLD2 antibody (A09768). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCBLD2 antigen affinity purified polyclonal antibody (A09768) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCBLD2 at approximately 120 kDa. The expected band size for DCBLD2 is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09768-dcbld2-primary-antibodies-ihc-testing-1.jpgAnti-DCBLD2 Antibody Picoband®IHC analysis of DCBLD2 using anti-DCBLD2 antibody (A09768). DCBLD2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DCBLD2 Antibody (A09768) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09768-dcbld2-primary-antibodies-if-testing-1.jpgAnti-DCBLD2 Antibody Picoband®IF analysis of DCBLD2 using anti-DCBLD2 antibody (A09768). DCBLD2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DCBLD2 Antibody (A09768) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09768-dcbld2-primary-antibodies-ip-testing-1.jpgAnti-DCBLD2 Antibody Picoband®Immunoprecipitating DCBLD2 in U251 whole cell lysate. Western blot analysis of DCBLD2 using anti-DCBLD2 antibody (A09768). Lane 1: U251 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DCBLD2 antibody in U251 whole cell lysate, Lane 3: anti-DCBLD2 antibody (2μg) + U251 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DCBLD2 antigen affinity purified polyclonal antibody (A09768) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DCBLD2 at approximately 120 kDa. The expected band size for DCBLD2 is at 85 kDa. https://www.bosterbio.com/catalog/product/view/id/1323222026-03-16T05:10:36+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323232026-03-16T05:10:36+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323242026-04-02T05:01:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09574-1-eif2s3-primary-antibodies-wb-testing-1.jpgAnti-EIF2S3 Antibody Picoband®Western blot analysis of EIF2S3 using anti-EIF2S3 antibody (A09574-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S3 antigen affinity purified polyclonal antibody (A09574-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EIF2S3 at approximately 51 kDa. The expected band size for EIF2S3 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09574-1-eif2s3-primary-antibodies-ihc-testing-1.jpgAnti-EIF2S3 Antibody Picoband®IHC analysis of EIF2S3 using anti-EIF2S3 antibody (A09574-1). EIF2S3 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF2S3 Antibody (A09574-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09574-1-eif2s3-primary-antibodies-fcm-testing-1.jpgAnti-EIF2S3 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-EIF2S3 antibody (A09574-1). Overlay histogram showing K562 cells stained with A09574-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF2S3 Antibody (A09574-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1323252026-03-16T05:10:36+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323262026-03-16T05:10:36+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323272026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05126-1-pde8b-primary-antibodies-wb-testing-1.jpgAnti-PDE8B Antibody Picoband®Western blot analysis of PDE8B using anti-PDE8B antibody (A05126-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE8B antigen affinity purified polyclonal antibody (A05126-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDE8B at approximately 99 kDa. The expected band size for PDE8B is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05126-1-pde8b-primary-antibodies-if-testing-1.jpgAnti-PDE8B Antibody Picoband®IF analysis of PDE8B using anti-PDE8B antibody (A05126-1). PDE8B was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PDE8B Antibody (A05126-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1323282026-03-16T05:10:36+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323292026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10613-farsa-primary-antibodies-wb-testing-1.jpgAnti-FARSA Antibody Picoband®Western blot analysis of FARSA using anti-FARSA antibody (A10613). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FARSA antigen affinity purified polyclonal antibody (A10613) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FARSA at approximately 58 kDa. The expected band size for FARSA is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10613-farsa-primary-antibodies-ihc-testing-1.jpgAnti-FARSA Antibody Picoband®IHC analysis of FARSA using anti-FARSA antibody (A10613). FARSA was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FARSA Antibody (A10613) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10613-farsa-primary-antibodies-ihc-testing-2.jpgAnti-FARSA Antibody Picoband®IHC analysis of FARSA using anti-FARSA antibody (A10613). FARSA was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FARSA Antibody (A10613) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10613-farsa-primary-antibodies-ihc-testing-3.jpgAnti-FARSA Antibody Picoband®IHC analysis of FARSA using anti-FARSA antibody (A10613). FARSA was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FARSA Antibody (A10613) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10613-farsa-primary-antibodies-ihc-testing-4.jpgAnti-FARSA Antibody Picoband®IHC analysis of FARSA using anti-FARSA antibody (A10613). FARSA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FARSA Antibody (A10613) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10613-farsa-primary-antibodies-ihc-testing-5.jpgAnti-FARSA Antibody Picoband®IHC analysis of FARSA using anti-FARSA antibody (A10613). FARSA was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FARSA Antibody (A10613) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10613-farsa-primary-antibodies-ihc-testing-6.jpgAnti-FARSA Antibody Picoband®IHC analysis of FARSA using anti-FARSA antibody (A10613). FARSA was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FARSA Antibody (A10613) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10613-farsa-primary-antibodies-ip-testing-1.jpgAnti-FARSA Antibody Picoband®Immunoprecipitating FARSA in K562 whole cell lysate. Western blot analysis of FARSA using anti-FARSA antibody (A10613). Lane 1: K562 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-FARSA antibody in K562 whole cell lysate, Lane 3: anti-FARSA antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FARSA antigen affinity purified polyclonal antibody (A10613) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FARSA at approximately 58 kDa. The expected band size for FARSA is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10613-farsa-primary-antibodies-fcm-testing-1.jpgAnti-FARSA Antibody Picoband®Flow Cytometry analysis of Hela cells using anti-FARSA antibody (A10613). Overlay histogram showing Hela cells stained with A10613 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FARSA Antibody (A10613, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1323302026-03-16T05:10:36+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323312026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11656-2-farsb-primary-antibodies-wb-testing-1.jpgAnti-FARSB Antibody Picoband®Western blot analysis of FARSB using anti-FARSB antibody (A11656-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FARSB antigen affinity purified polyclonal antibody (A11656-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FARSB at approximately 70 kDa. The expected band size for FARSB is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11656-2-farsb-primary-antibodies-ihc-testing-1.jpgAnti-FARSB Antibody Picoband®IHC analysis of FARSB using anti-FARSB antibody (A11656-2). FARSB was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FARSB Antibody (A11656-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11656-2-farsb-primary-antibodies-ip-testing-1.jpgAnti-FARSB Antibody Picoband®Immunoprecipitating FARSB in K562 whole cell lysate. Western blot analysis of FARSB using anti-FARSB antibody (A11656-2). Lane 1: K562 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-FARSB antibody in K562 whole cell lysate, Lane 3: anti-FARSB antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FARSB antigen affinity purified polyclonal antibody (A11656-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FARSB at approximately 70 kDa. The expected band size for FARSB is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11656-2-farsb-primary-antibodies-fcm-testing-1.jpgAnti-FARSB Antibody Picoband®Flow Cytometry analysis of Hela cells using anti-FARSB antibody (A11656-2). Overlay histogram showing Hela cells stained with A11656-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FARSB Antibody (A11656-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1323322026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07906-1-dzip1-primary-antibodies-wb-testing-1.jpgAnti-DZIP1 Antibody Picoband®Western blot analysis of DZIP1 using anti-DZIP1 antibody (A07906-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DZIP1 antigen affinity purified polyclonal antibody (A07906-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DZIP1 at approximately 113 kDa. The expected band size for DZIP1 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07906-1-dzip1-primary-antibodies-ihc-testing-1.jpgAnti-DZIP1 Antibody Picoband®IHC analysis of DZIP1 using anti-DZIP1 antibody (A07906-1). DZIP1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DZIP1 Antibody (A07906-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07906-1-dzip1-primary-antibodies-ihc-testing-2.jpgAnti-DZIP1 Antibody Picoband®IHC analysis of DZIP1 using anti-DZIP1 antibody (A07906-1). DZIP1 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DZIP1 Antibody (A07906-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07906-1-dzip1-primary-antibodies-ihc-testing-3.jpgAnti-DZIP1 Antibody Picoband®IHC analysis of DZIP1 using anti-DZIP1 antibody (A07906-1). DZIP1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DZIP1 Antibody (A07906-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1323332026-03-16T05:10:36+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323342026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323352026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323362026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323372026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323382026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323392026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323402026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323412026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323422026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323432026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323442026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323452026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323462026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323472026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323482026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323492026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323502026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323512026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323522026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323532026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12109-2-pole3-primary-antibodies-wb-testing-1.jpgAnti-POLE3 Antibody Picoband®Western blot analysis of POLE3 using anti-POLE3 antibody (A12109-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLE3 antigen affinity purified polyclonal antibody (A12109-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POLE3 at approximately 17 kDa. The expected band size for POLE3 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12109-2-pole3-primary-antibodies-ihc-testing-1.jpgAnti-POLE3 Antibody Picoband®IHC analysis of POLE3 using anti-POLE3 antibody (A12109-2). POLE3 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLE3 Antibody (A12109-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12109-2-pole3-primary-antibodies-ihc-testing-2.jpgAnti-POLE3 Antibody Picoband®IHC analysis of POLE3 using anti-POLE3 antibody (A12109-2). POLE3 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-POLE3 Antibody (A12109-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1323542026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323552026-03-16T05:10:37+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323562026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323572026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323582026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12980-dnaaf5-primary-antibodies-wb-testing-1.jpgAnti-HEATR2/DNAAF5 Antibody Picoband®Western blot analysis of HEATR2/DNAAF5 using anti-HEATR2/DNAAF5 antibody (A12980). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEATR2/DNAAF5 antigen affinity purified polyclonal antibody (A12980) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HEATR2/DNAAF5 at approximately 90-100 kDa. The expected band size for HEATR2/DNAAF5 is at 94 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12980-dnaaf5-primary-antibodies-ihc-testing-1.jpgAnti-HEATR2/DNAAF5 Antibody Picoband®IHC analysis of HEATR2/DNAAF5 using anti-HEATR2/DNAAF5 antibody (A12980). HEATR2/DNAAF5 was detected in a paraffin-embedded section of human fallopian tube tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HEATR2/DNAAF5 Antibody (A12980) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1323592026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323602026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323612026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323622026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323632026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323642026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323652026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323662026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323672026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323682026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323692026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323702026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323712026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06223-1-lrsam1-primary-antibodies-wb-testing-1.jpgAnti-LRSAM1 AntibodyWestern blot analysis of LRSAM1 using anti-LRSAM1 antibody (A06223-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRSAM1 antigen affinity purified polyclonal antibody (A06223-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LRSAM1 at approximately 84 kDa. The expected band size for LRSAM1 is at 84 kDa. https://www.bosterbio.com/catalog/product/view/id/1323722026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323732026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323742026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323752026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323762026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323772026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323782026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323792026-03-16T05:10:38+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323802026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323812026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323822026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323832026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323842026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323852026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323862026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323872026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323882026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323892026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323902026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323912026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323922026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323932026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323942026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323952026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323962026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323972026-03-16T05:10:39+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323982026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1323992026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324002026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324012026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324022026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324032026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324042026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324052026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324062026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324072026-03-16T05:10:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09240-obfc2b-primary-antibodies-wb-testing-1.jpgAnti-OBFC2B/NABP2 AntibodyWestern blot analysis of OBFC2B using anti-OBFC2B antibody (A09240). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OBFC2B antigen affinity purified polyclonal antibody (A09240) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OBFC2B at approximately 33 kDa. The expected band size for OBFC2B is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09240-obfc2b-primary-antibodies-ihc-testing-1.jpgAnti-OBFC2B/NABP2 AntibodyIHC analysis of OBFC2B using anti-OBFC2B antibody (A09240). OBFC2B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-OBFC2B Antibody (A09240) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09240-obfc2b-primary-antibodies-ihc-testing-2.jpgAnti-OBFC2B/NABP2 AntibodyIHC analysis of OBFC2B using anti-OBFC2B antibody (A09240). OBFC2B was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-OBFC2B Antibody (A09240) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09240-obfc2b-primary-antibodies-ihc-testing-3.jpgAnti-OBFC2B/NABP2 AntibodyIHC analysis of OBFC2B using anti-OBFC2B antibody (A09240). OBFC2B was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-OBFC2B Antibody (A09240) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09240-obfc2b-primary-antibodies-ihc-testing-4.jpgAnti-OBFC2B/NABP2 AntibodyIHC analysis of OBFC2B using anti-OBFC2B antibody (A09240). OBFC2B was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-OBFC2B Antibody (A09240) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09240-obfc2b-primary-antibodies-ihc-testing-5.jpgAnti-OBFC2B/NABP2 AntibodyIHC analysis of OBFC2B using anti-OBFC2B antibody (A09240). OBFC2B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-OBFC2B Antibody (A09240) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09240-obfc2b-primary-antibodies-ihc-testing-6.jpgAnti-OBFC2B/NABP2 AntibodyIHC analysis of OBFC2B using anti-OBFC2B antibody (A09240). OBFC2B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-OBFC2B Antibody (A09240) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1324082026-03-16T05:10:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15301-2-ccdc12-primary-antibodies-wb-testing-1.jpgAnti-CCDC12 AntibodyWestern blot analysis of CCDC12 using anti-CCDC12 antibody (A15301-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC12 antigen affinity purified polyclonal antibody (A15301-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CCDC12 at approximately 22 kDa. The expected band size for CCDC12 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15301-2-ccdc12-primary-antibodies-ihc-testing-1.jpgAnti-CCDC12 AntibodyIHC analysis of CCDC12 using anti-CCDC12 antibody (A15301-2). CCDC12 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CCDC12 Antibody (A15301-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15301-2-ccdc12-primary-antibodies-ihc-testing-2.jpgAnti-CCDC12 AntibodyIHC analysis of CCDC12 using anti-CCDC12 antibody (A15301-2). CCDC12 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CCDC12 Antibody (A15301-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15301-2-ccdc12-primary-antibodies-ihc-testing-3.jpgAnti-CCDC12 AntibodyIHC analysis of CCDC12 using anti-CCDC12 antibody (A15301-2). CCDC12 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CCDC12 Antibody (A15301-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15301-2-ccdc12-primary-antibodies-ihc-testing-4.jpgAnti-CCDC12 AntibodyIHC analysis of CCDC12 using anti-CCDC12 antibody (A15301-2). CCDC12 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CCDC12 Antibody (A15301-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1324092026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324102026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324112026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324122026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324132026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324142026-03-16T05:10:40+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324152026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324162026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324172026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324182026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324192026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324202026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324212026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324222026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324232026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324242026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324252026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06738-2-pla2g1b-primary-antibodies-wb-testing-1.jpgAnti-PLA2G1B AntibodyWestern blot analysis of PLA2G1B using anti-PLA2G1B antibody (A06738-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G1B antigen affinity purified polyclonal antibody (A06738-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLA2G1B at approximately 16 kDa. The expected band size for PLA2G1B is at 16 kDa. https://www.bosterbio.com/catalog/product/view/id/1324262026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324272026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324282026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10500-1-ppig-primary-antibodies-wb-testing-1.jpgAnti-PPIG Antibody Picoband®Western blot analysis of PPIG using anti-PPIG antibody (A10500-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPIG antigen affinity purified polyclonal antibody (A10500-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPIG at approximately 120 kDa. The expected band size for PPIG is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10500-1-ppig-primary-antibodies-ihc-testing-1.jpgAnti-PPIG Antibody Picoband®IHC analysis of PPIG using anti-PPIG antibody (A10500-1). PPIG was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPIG Antibody (A10500-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10500-1-ppig-primary-antibodies-ihc-testing-2.jpgAnti-PPIG Antibody Picoband®IHC analysis of PPIG using anti-PPIG antibody (A10500-1). PPIG was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPIG Antibody (A10500-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10500-1-ppig-primary-antibodies-ihc-testing-3.jpgAnti-PPIG Antibody Picoband®IHC analysis of PPIG using anti-PPIG antibody (A10500-1). PPIG was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPIG Antibody (A10500-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10500-1-ppig-primary-antibodies-ihc-testing-4.jpgAnti-PPIG Antibody Picoband®IHC analysis of PPIG using anti-PPIG antibody (A10500-1). PPIG was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPIG Antibody (A10500-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10500-1-ppig-primary-antibodies-ihc-testing-5.jpgAnti-PPIG Antibody Picoband®IHC analysis of PPIG using anti-PPIG antibody (A10500-1). PPIG was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPIG Antibody (A10500-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10500-1-ppig-primary-antibodies-ihc-testing-6.jpgAnti-PPIG Antibody Picoband®IHC analysis of PPIG using anti-PPIG antibody (A10500-1). PPIG was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPIG Antibody (A10500-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10500-1-ppig-primary-antibodies-ihc-testing-7.jpgAnti-PPIG Antibody Picoband®IHC analysis of PPIG using anti-PPIG antibody (A10500-1). PPIG was detected in a paraffin-embedded section of human esophageal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPIG Antibody (A10500-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10500-1-ppig-primary-antibodies-ihc-testing-8.jpgAnti-PPIG Antibody Picoband®IHC analysis of PPIG using anti-PPIG antibody (A10500-1). PPIG was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPIG Antibody (A10500-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10500-1-ppig-primary-antibodies-ihc-testing-9.jpgAnti-PPIG Antibody Picoband®IHC analysis of PPIG using anti-PPIG antibody (A10500-1). PPIG was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPIG Antibody (A10500-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10500-1-ppig-primary-antibodies-ihc-testing-10.jpgAnti-PPIG Antibody Picoband®IHC analysis of PPIG using anti-PPIG antibody (A10500-1). PPIG was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPIG Antibody (A10500-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10500-1-ppig-primary-antibodies-ihc-testing-11.jpgAnti-PPIG Antibody Picoband®IHC analysis of PPIG using anti-PPIG antibody (A10500-1). PPIG was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPIG Antibody (A10500-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1324292026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324302026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324312026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324322026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324332026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324342026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324352026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324362026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324372026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324382026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324392026-03-16T05:10:41+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324402026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06403-3-usp30-primary-antibodies-wb-testing-1.jpgAnti-USP30 AntibodyWestern blot analysis of USP30 using anti-USP30 antibody (A06403-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP30 antigen affinity purified polyclonal antibody (A06403-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for USP30 at approximately 72 kDa. The expected band size for USP30 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06403-3-usp30-primary-antibodies-ihc-testing-1.jpgAnti-USP30 AntibodyIHC analysis of USP30 using anti-USP30 antibody (A06403-3). USP30 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-USP30 Antibody (A06403-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1324412026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324422026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324432026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324442026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324452026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324462026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324472026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324482026-03-16T05:10:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12402-1-rbp7-primary-antibodies-wb-testing-1.jpgAnti-RBP7 AntibodyWestern blot analysis of RBP7 using anti-RBP7 antibody (A12402-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat adipose tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP7 antigen affinity purified polyclonal antibody (A12402-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBP7 at approximately 15 kDa. The expected band size for RBP7 is at 16 kDa. https://www.bosterbio.com/catalog/product/view/id/1324492026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324502026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324512026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324522026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324532026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324542026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324552026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324562026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1324622026-03-13T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02461-1-gsto1-primary-antibodies-wb-testing-1_2.jpgAnti-GSTO1 Antibody Western blot analysis of GSTO1 using anti-GSTO1 antibody (A02461-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTO1 antigen affinity purified polyclonal antibody (A02461-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSTO1 at approximately 28 kDa. The expected band size for GSTO1 is at 28 kDa. https://www.bosterbio.com/catalog/product/view/id/1324632026-03-17T05:17:27+00:00daily0.9 https://www.bosterbio.com/products/pap-pen-ar1181-boster.html2026-03-10T04:42:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1181.jpgPAP penhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1181-1.jpgPAP penhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1181-2.jpgPAP pen https://www.bosterbio.com/catalog/product/view/id/1324652026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10868-1-tuft1-primary-antibodies-wb-testing-1.jpgAnti-TUFT1 Antibody Picoband®Western blot analysis of TUFT1 using anti-TUFT1 antibody (A10868-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUFT1 antigen affinity purified polyclonal antibody (A10868-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TUFT1 at approximately 44 kDa. The expected band size for TUFT1 is at 44 kDa. https://www.bosterbio.com/catalog/product/view/id/1324662026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07427-1-ube2g2-primary-antibodies-wb-testing-1.jpgAnti-UBE2G2 Antibody Picoband®Western blot analysis of UBE2G2 using anti-UBE2G2 antibody (A07427-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat L6 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2G2 antigen affinity purified polyclonal antibody (A07427-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBE2G2 at approximately 17 kDa. The expected band size for UBE2G2 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07427-1-ube2g2-primary-antibodies-ihc-testing-1.jpgAnti-UBE2G2 Antibody Picoband®IHC analysis of UBE2G2 using anti-UBE2G2 antibody (A07427-1). UBE2G2 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBE2G2 Antibody (A07427-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07427-1-ube2g2-primary-antibodies-fcm-testing-1.jpgAnti-UBE2G2 Antibody Picoband®Flow Cytometry analysis of 3T3-L1 cells using anti-UBE2G2 antibody (A07427-1). Overlay histogram showing 3T3-L1 cells stained with A07427-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2G2 Antibody (A07427-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07427-1-ube2g2-primary-antibodies-fcm-testing-2.jpgAnti-UBE2G2 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-UBE2G2 antibody (A07427-1). Overlay histogram showing RT4 cells stained with A07427-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2G2 Antibody (A07427-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324672026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17234-1-hdhd3-primary-antibodies-wb-testing-1.jpgAnti-HDHD3 Antibody Picoband®Western blot analysis of HDHD3 using anti-HDHD3 antibody (A17234-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: rat small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDHD3 antigen affinity purified polyclonal antibody (A17234-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HDHD3 at approximately 28 kDa. The expected band size for HDHD3 is at 28 kDa. https://www.bosterbio.com/catalog/product/view/id/1324682026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00679-1-trio-primary-antibodies-ihc-testing-1.jpgAnti-TRIO AntibodyIHC analysis of TRIO using anti-TRIO antibody (A00679-1). TRIO was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIO Antibody (A00679-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00679-1-trio-primary-antibodies-ihc-testing-2.jpgAnti-TRIO AntibodyIHC analysis of TRIO using anti-TRIO antibody (A00679-1). TRIO was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRIO Antibody (A00679-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00679-1-trio-primary-antibodies-if-testing-1.jpgAnti-TRIO AntibodyIF analysis of TRIO using anti-TRIO antibody (A00679-1). TRIO was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRIO Antibody (A00679-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00679-1-trio-primary-antibodies-if-testing-2.jpgAnti-TRIO AntibodyIF analysis of TRIO using anti-TRIO antibody (A00679-1). TRIO was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TRIO Antibody (A00679-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00679-1-trio-primary-antibodies-fcm-testing-1.jpgAnti-TRIO AntibodyFlow Cytometry analysis of U2OS cells using anti-TRIO antibody (A00679-1). Overlay histogram showing U2OS cells stained with A00679-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIO Antibody (A00679-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324692026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08950-1-dcaf6-primary-antibodies-wb-testing-1.jpgAnti-DCAF6 Antibody Picoband®Western blot analysis of DCAF6 using anti-DCAF6 antibody (A08950-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCAF6 antigen affinity purified polyclonal antibody (A08950-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCAF6 at approximately 96 kDa. The expected band size for DCAF6 is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08950-1-dcaf6-primary-antibodies-if-testing-1.jpgAnti-DCAF6 Antibody Picoband®IF analysis of DCAF6 using anti-DCAF6 antibody (A08950-1) and anti-Alpha Tubulin antibody (M03989-3). DCAF6 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DCAF6 Antibody (A08950-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08950-1-dcaf6-primary-antibodies-fcm-testing-1.jpgAnti-DCAF6 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-DCAF6 antibody (A08950-1). Overlay histogram showing HEL cells stained with A08950-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DCAF6 Antibody (A08950-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324702026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08577-1-dcaf7-primary-antibodies-wb-testing-1.jpgAnti-DCAF7 Antibody Picoband®Western blot analysis of DCAF7 using anti-DCAF7 antibody (A08577-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCAF7 antigen affinity purified polyclonal antibody (A08577-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCAF7 at approximately 39 kDa. The expected band size for DCAF7 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08577-1-dcaf7-primary-antibodies-if-testing-1.jpgAnti-DCAF7 Antibody Picoband®IF analysis of DCAF7 using anti-DCAF7 antibody (A08577-1) and anti-Alpha Tubulin antibody (M03989-3). DCAF7 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DCAF7 Antibody (A08577-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1324712026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04232-2-egr3-primary-antibodies-wb-testing-1.jpgAnti-EGR3 Antibody Picoband®Western blot analysis of EGR3 using anti-EGR3 antibody (A04232-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGR3 antigen affinity purified polyclonal antibody (A04232-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EGR3 at approximately 43 kDa. The expected band size for EGR3 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04232-2-egr3-primary-antibodies-ihc-testing-1.jpgAnti-EGR3 Antibody Picoband®IHC analysis of EGR3 using anti-EGR3 antibody (A04232-2). EGR3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EGR3 Antibody (A04232-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04232-2-egr3-primary-antibodies-ihc-testing-2.jpgAnti-EGR3 Antibody Picoband®IHC analysis of EGR3 using anti-EGR3 antibody (A04232-2). EGR3 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EGR3 Antibody (A04232-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04232-2-egr3-primary-antibodies-ihc-testing-3.jpgAnti-EGR3 Antibody Picoband®IHC analysis of EGR3 using anti-EGR3 antibody (A04232-2). EGR3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EGR3 Antibody (A04232-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1324722026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02437-2-ffar3-primary-antibodies-wb-testing-1.jpgAnti-FFAR3 Antibody Picoband®Western blot analysis of FFAR3 using anti-FFAR3 antibody (A02437-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FFAR3 antigen affinity purified polyclonal antibody (A02437-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FFAR3 at approximately 39 kDa. The expected band size for FFAR3 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02437-2-ffar3-primary-antibodies-fcm-testing-1.jpgAnti-FFAR3 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-FFAR3 antibody (A02437-2). Overlay histogram showing SH-SY5Y cells stained with A02437-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-FFAR3 Antibody (A02437-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324742026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13550-map10-primary-antibodies-wb-testing-1.jpgAnti-MAP10 Antibody Picoband®Western blot analysis of MAP10 using anti-MAP10 antibody (A13550). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Reh whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP10 antigen affinity purified polyclonal antibody (A13550) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAP10 at approximately 120 kDa. The expected band size for MAP10 is at 100 kDa. https://www.bosterbio.com/catalog/product/view/id/1324752026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14622-1-rsbn1l-primary-antibodies-wb-testing-1.jpgAnti-RSBN1L Antibody Picoband®Western blot analysis of RSBN1L using anti-RSBN1L antibody (A14622-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSBN1L antigen affinity purified polyclonal antibody (A14622-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RSBN1L at approximately 100 kDa. The expected band size for RSBN1L is at 95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14622-1-rsbn1l-primary-antibodies-fcm-testing-1.jpgAnti-RSBN1L Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-RSBN1L antibody (A14622-1). Overlay histogram showing HEL cells stained with A14622-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RSBN1L Antibody (A14622-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324762026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10894-1-pdcl2-primary-antibodies-wb-testing-1.jpgAnti-PDCL2 Antibody Picoband®Western blot analysis of PDCL2 using anti-PDCL2 antibody (A10894-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCL2 antigen affinity purified polyclonal antibody (A10894-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDCL2 at approximately 35 kDa. The expected band size for PDCL2 is at 28 kDa. https://www.bosterbio.com/catalog/product/view/id/1324772026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10000-1-pdcl3-primary-antibodies-wb-testing-1.jpgAnti-PDCL3 Antibody Picoband®Western blot analysis of PDCL3 using anti-PDCL3 antibody (A10000-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A2780 whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human NCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDCL3 antigen affinity purified polyclonal antibody (A10000-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDCL3 at approximately 34 kDa. The expected band size for PDCL3 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10000-1-pdcl3-primary-antibodies-ihc-testing-1.jpgAnti-PDCL3 Antibody Picoband®IHC analysis of PDCL3 using anti-PDCL3 antibody (A10000-1). PDCL3 was detected in a paraffin-embedded section of rat bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDCL3 Antibody (A10000-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10000-1-pdcl3-primary-antibodies-ihc-testing-2.jpgAnti-PDCL3 Antibody Picoband®IHC analysis of PDCL3 using anti-PDCL3 antibody (A10000-1). PDCL3 was detected in a paraffin-embedded section of mouse bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDCL3 Antibody (A10000-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10000-1-pdcl3-primary-antibodies-if-testing-1.jpgAnti-PDCL3 Antibody Picoband®IF analysis of PDCL3 using anti-PDCL3 antibody (A10000-1). PDCL3 was detected in a paraffin-embedded section of rat bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PDCL3 Antibody (A10000-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10000-1-pdcl3-primary-antibodies-if-testing-2.jpgAnti-PDCL3 Antibody Picoband®IF analysis of PDCL3 using anti-PDCL3 antibody (A10000-1). PDCL3 was detected in a paraffin-embedded section of mouse bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PDCL3 Antibody (A10000-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10000-1-pdcl3-primary-antibodies-if-testing-3.jpgAnti-PDCL3 Antibody Picoband®IF analysis of PDCL3 using anti-PDCL3 antibody (A10000-1) and anti-Alpha Tubulin antibody (M03989-3). PDCL3 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PDCL3 Antibody (A10000-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10000-1-pdcl3-primary-antibodies-ip-testing-1.jpgAnti-PDCL3 Antibody Picoband®Immunoprecipitating PDCL3 in MCF-7 whole cell lysate. Western blot analysis of PDCL3 using anti-PDCL3 antibody (A10000-1). Lane 1: MCF-7 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-PDCL3 antibody in MCF-7 whole cell lysate, Lane 3: anti-PDCL3 antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PDCL3 antigen affinity purified polyclonal antibody (A10000-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PDCL3 at approximately 34 kDa. The expected band size for PDCL3 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10000-1-pdcl3-primary-antibodies-fcm-testing-1.jpgAnti-PDCL3 Antibody Picoband®Flow Cytometry analysis of A2780 cells using anti-PDCL3 antibody (A10000-1). Overlay histogram showing A2780 cells stained with A10000-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDCL3 Antibody (A10000-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324782026-03-17T05:17:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07630-3-pde4c-primary-antibodies-wb-testing-1.jpgAnti-PDE4C Antibody Picoband®Western blot analysis of PDE4C using anti-PDE4C antibody (A07630-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE4C antigen affinity purified polyclonal antibody (A07630-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDE4C at approximately 80 kDa. The expected band size for PDE4C is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07630-3-pde4c-primary-antibodies-fcm-testing-1.jpgAnti-PDE4C Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-PDE4C antibody (A07630-3). Overlay histogram showing K562 cells stained with A07630-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PDE4C Antibody (A07630-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324792026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03323-1-pde3b-primary-antibodies-wb-testing-1.jpgAnti-PDE3B Antibody Picoband®Western blot analysis of PDE3B using anti-PDE3B antibody (A03323-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE3B antigen affinity purified polyclonal antibody (A03323-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDE3B at approximately 130 kDa. The expected band size for PDE3B is at 124 kDa. https://www.bosterbio.com/catalog/product/view/id/1324802026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13080-1-ttll10-primary-antibodies-wb-testing-1.jpgAnti-TTLL10 Antibody Picoband®Western blot analysis of TTLL10 using anti-TTLL10 antibody (A13080-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL10 antigen affinity purified polyclonal antibody (A13080-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TTLL10 at approximately 75 kDa. The expected band size for TTLL10 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13080-1-ttll10-primary-antibodies-ihc-testing-1.jpgAnti-TTLL10 Antibody Picoband®IHC analysis of TTLL10 using anti-TTLL10 antibody (A13080-1). TTLL10 was detected in a paraffin-embedded section of human fallopian tube tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTLL10 Antibody (A13080-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13080-1-ttll10-primary-antibodies-if-testing-1.jpgAnti-TTLL10 Antibody Picoband®IF analysis of TTLL10 using anti-TTLL10 antibody (A13080-1). TTLL10 was detected in a paraffin-embedded section of human fallopian tube tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TTLL10 Antibody (A13080-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13080-1-ttll10-primary-antibodies-fcm-testing-1.jpgAnti-TTLL10 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-TTLL10 antibody (A13080-1). Overlay histogram showing HEL cells stained with A13080-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTLL10 Antibody (A13080-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324812026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10763-1-grid1-primary-antibodies-wb-testing-1.jpgAnti-GRID1 Antibody Picoband®Western blot analysis of GRID1 using anti-GRID1 antibody (A10763-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRID1 antigen affinity purified polyclonal antibody (A10763-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRID1 at approximately 112 kDa. The expected band size for GRID1 is at 112 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10763-1-grid1-primary-antibodies-ihc-testing-1.jpgAnti-GRID1 Antibody Picoband®IHC analysis of GRID1 using anti-GRID1 antibody (A10763-1). GRID1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRID1 Antibody (A10763-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10763-1-grid1-primary-antibodies-fcm-testing-1.jpgAnti-GRID1 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-GRID1 antibody (A10763-1). Overlay histogram showing SH-SY5Y cells stained with A10763-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GRID1 Antibody (A10763-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324822026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08165-2-fzd9-primary-antibodies-wb-testing-1.jpgAnti-Frizzled 9/FZD9 Antibody Picoband®Western blot analysis of Frizzled 9/FZD9 using anti-Frizzled 9/FZD9 antibody (A08165-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse small intestine tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Frizzled 9/FZD9 antigen affinity purified polyclonal antibody (A08165-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Frizzled 9/FZD9 at approximately 70 kDa. The expected band size for Frizzled 9/FZD9 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08165-2-fzd9-primary-antibodies-ihc-testing-1.jpgAnti-Frizzled 9/FZD9 Antibody Picoband®IHC analysis of Frizzled 9/FZD9 using anti-Frizzled 9/FZD9 antibody (A08165-2). Frizzled 9/FZD9 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Frizzled 9/FZD9 Antibody (A08165-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08165-2-fzd9-primary-antibodies-ihc-testing-2.jpgAnti-Frizzled 9/FZD9 Antibody Picoband®IHC analysis of Frizzled 9/FZD9 using anti-Frizzled 9/FZD9 antibody (A08165-2). Frizzled 9/FZD9 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Frizzled 9/FZD9 Antibody (A08165-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08165-2-fzd9-primary-antibodies-if-testing-1.jpgAnti-Frizzled 9/FZD9 Antibody Picoband®IF analysis of Frizzled 9/FZD9 using anti-Frizzled 9/FZD9 antibody (A08165-2). Frizzled 9/FZD9 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Frizzled 9/FZD9 Antibody (A08165-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08165-2-fzd9-primary-antibodies-if-testing-2.jpgAnti-Frizzled 9/FZD9 Antibody Picoband®IF analysis of Frizzled 9/FZD9 using anti-Frizzled 9/FZD9 antibody (A08165-2). Frizzled 9/FZD9 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Frizzled 9/FZD9 Antibody (A08165-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08165-2-fzd9-primary-antibodies-fcm-testing-1.jpgAnti-Frizzled 9/FZD9 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-Frizzled 9/FZD9 antibody (A08165-2). Overlay histogram showing THP-1 cells stained with A08165-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Frizzled 9/FZD9 Antibody (A08165-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324832026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01138-2-scn4a-primary-antibodies-wb-testing-1.jpgAnti-SCN4A Antibody Picoband®Western blot analysis of SCN4A using anti-SCN4A antibody (A01138-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse small intestine tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCN4A antigen affinity purified polyclonal antibody (A01138-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SCN4A at approximately 290 kDa. The expected band size for SCN4A is at 208 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01138-2-scn4a-primary-antibodies-if-testing-1.jpgAnti-SCN4A Antibody Picoband®IF analysis of SCN4A using anti-SCN4A antibody (A01138-2). SCN4A was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SCN4A Antibody (A01138-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01138-2-scn4a-primary-antibodies-fcm-testing-1.jpgAnti-SCN4A Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-SCN4A antibody (A01138-2). Overlay histogram showing THP-1 cells stained with A01138-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SCN4A Antibody (A01138-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01138-2-scn4a-primary-antibodies-fcm-testing-2.jpgAnti-SCN4A Antibody Picoband®Flow Cytometry analysis of U2OS cells using anti-SCN4A antibody (A01138-2). Overlay histogram showing U2OS cells stained with A01138-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SCN4A Antibody (A01138-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324842026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08614-2-nup35-primary-antibodies-wb-testing-1.jpgAnti-NUP35 Antibody Picoband®Western blot analysis of NUP35 using anti-NUP35 antibody (A08614-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP35 antigen affinity purified polyclonal antibody (A08614-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NUP35 at approximately 35 kDa. The expected band size for NUP35 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08614-2-nup35-primary-antibodies-if-testing-1.jpgAnti-NUP35 Antibody Picoband®IF analysis of NUP35 using anti-NUP35 antibody (A08614-2). NUP35 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NUP35 Antibody (A08614-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08614-2-nup35-primary-antibodies-fcm-testing-1.jpgAnti-NUP35 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-NUP35 antibody (A08614-2). Overlay histogram showing K562 cells stained with A08614-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUP35 Antibody (A08614-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324852026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06324-2-ube3c-primary-antibodies-wb-testing-1.jpgAnti-UBE3C Antibody Picoband®Western blot analysis of UBE3C using anti-UBE3C antibody (A06324-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse small intestine tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE3C antigen affinity purified polyclonal antibody (A06324-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBE3C at approximately 70 kDa. The expected band size for UBE3C is at 124 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06324-2-ube3c-primary-antibodies-ihc-testing-1.jpgAnti-UBE3C Antibody Picoband®IHC analysis of UBE3C using anti-UBE3C antibody (A06324-2). UBE3C was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBE3C Antibody (A06324-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1324862026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14170-1-ubr7-primary-antibodies-wb-testing-1.jpgAnti-UBR7 Antibody Picoband®Western blot analysis of UBR7 using anti-UBR7 antibody (A14170-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBR7 antigen affinity purified polyclonal antibody (A14170-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBR7 at approximately 55 kDa. The expected band size for UBR7 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14170-1-ubr7-primary-antibodies-ihc-testing-1.jpgAnti-UBR7 Antibody Picoband®IHC analysis of UBR7 using anti-UBR7 antibody (A14170-1). UBR7 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBR7 Antibody (A14170-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14170-1-ubr7-primary-antibodies-ihc-testing-2.jpgAnti-UBR7 Antibody Picoband®IHC analysis of UBR7 using anti-UBR7 antibody (A14170-1). UBR7 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UBR7 Antibody (A14170-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14170-1-ubr7-primary-antibodies-ip-testing-1.jpgAnti-UBR7 Antibody Picoband®Immunoprecipitating UBR7 in MCF-7 whole cell lysate. Western blot analysis of UBR7 using anti-UBR7 antibody (A14170-1). Lane 1: MCF-7 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-UBR7 antibody in MCF-7 whole cell lysate, Lane 3: anti-UBR7 antibody (2μg) + MCF-7 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UBR7 antigen affinity purified polyclonal antibody (A14170-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for UBR7 at approximately 55 kDa. The expected band size for UBR7 is at 48 kDa. https://www.bosterbio.com/catalog/product/view/id/1324872026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06466-2-pde6d-primary-antibodies-wb-testing-1.jpgAnti-PDE6D Antibody Picoband®Western blot analysis of PDE6D using anti-PDE6D antibody (A06466-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat eye tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE6D antigen affinity purified polyclonal antibody (A06466-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDE6D at approximately 15 kDa. The expected band size for PDE6D is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06466-2-pde6d-primary-antibodies-ihc-testing-1.jpgAnti-PDE6D Antibody Picoband®IHC analysis of PDE6D using anti-PDE6D antibody (A06466-2). PDE6D was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDE6D Antibody (A06466-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06466-2-pde6d-primary-antibodies-ihc-testing-2.jpgAnti-PDE6D Antibody Picoband®IHC analysis of PDE6D using anti-PDE6D antibody (A06466-2). PDE6D was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDE6D Antibody (A06466-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06466-2-pde6d-primary-antibodies-if-testing-1.jpgAnti-PDE6D Antibody Picoband®IF analysis of PDE6D using anti-PDE6D antibody (A06466-2). PDE6D was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PDE6D Antibody (A06466-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06466-2-pde6d-primary-antibodies-if-testing-2.jpgAnti-PDE6D Antibody Picoband®IF analysis of PDE6D using anti-PDE6D antibody (A06466-2). PDE6D was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PDE6D Antibody (A06466-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1324882026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04832-3-pdlim1-primary-antibodies-wb-testing-1.jpgAnti-CLP36/PDLIM1 Antibody Picoband®Western blot analysis of CLP36/PDLIM1 using anti-CLP36/PDLIM1 antibody (A04832-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat small intestine tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse small intestine tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLP36/PDLIM1 antigen affinity purified polyclonal antibody (A04832-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CLP36/PDLIM1 at approximately 36 kDa. The expected band size for CLP36/PDLIM1 is at 36 kDa. https://www.bosterbio.com/catalog/product/view/id/1324892026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06506-1-pdlim2-primary-antibodies-wb-testing-1.jpgAnti-PDLIM2 Antibody Picoband®Western blot analysis of PDLIM2 using anti-PDLIM2 antibody (A06506-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDLIM2 antigen affinity purified polyclonal antibody (A06506-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDLIM2 at approximately 37 kDa. The expected band size for PDLIM2 is at 37 kDa. https://www.bosterbio.com/catalog/product/view/id/1324902026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01785-1-pemt-primary-antibodies-wb-testing-1.jpgAnti-PEMT Antibody Picoband®Western blot analysis of PEMT using anti-PEMT antibody (A01785-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEMT antigen affinity purified polyclonal antibody (A01785-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PEMT at approximately 17 kDa. The expected band size for PEMT is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01785-1-pemt-primary-antibodies-ihc-testing-1.jpgAnti-PEMT Antibody Picoband®IHC analysis of PEMT using anti-PEMT antibody (A01785-1). PEMT was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PEMT Antibody (A01785-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01785-1-pemt-primary-antibodies-ihc-testing-2.jpgAnti-PEMT Antibody Picoband®IHC analysis of PEMT using anti-PEMT antibody (A01785-1). PEMT was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PEMT Antibody (A01785-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01785-1-pemt-primary-antibodies-fcm-testing-1.jpgAnti-PEMT Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-PEMT antibody (A01785-1). Overlay histogram showing MCF-7 cells stained with A01785-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PEMT Antibody (A01785-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324912026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05543-1-ufm1-primary-antibodies-wb-testing-1.jpgAnti-UFM1 Antibody Picoband®Western blot analysis of UFM1 using anti-UFM1 antibody (A05543-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UFM1 antigen affinity purified polyclonal antibody (A05543-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UFM1 at approximately 10 kDa. The expected band size for UFM1 is at 10 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05543-1-ufm1-primary-antibodies-ihc-testing-1.jpgAnti-UFM1 Antibody Picoband®IHC analysis of UFM1 using anti-UFM1 antibody (A05543-1). UFM1 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UFM1 Antibody (A05543-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05543-1-ufm1-primary-antibodies-ihc-testing-2.jpgAnti-UFM1 Antibody Picoband®IHC analysis of UFM1 using anti-UFM1 antibody (A05543-1). UFM1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-UFM1 Antibody (A05543-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05543-1-ufm1-primary-antibodies-if-testing-1.jpgAnti-UFM1 Antibody Picoband®IF analysis of UFM1 using anti-UFM1 antibody (A05543-1). UFM1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-UFM1 Antibody (A05543-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05543-1-ufm1-primary-antibodies-fcm-testing-1.jpgAnti-UFM1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-UFM1 antibody (A05543-1). Overlay histogram showing HepG2 cells stained with A05543-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UFM1 Antibody (A05543-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05543-1-ufm1-primary-antibodies-fcm-testing-2.jpgAnti-UFM1 Antibody Picoband®Flow Cytometry analysis of RH35 cells using anti-UFM1 antibody (A05543-1). Overlay histogram showing RH35 cells stained with A05543-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UFM1 Antibody (A05543-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05543-1-ufm1-primary-antibodies-fcm-testing-3.jpgAnti-UFM1 Antibody Picoband®Flow Cytometry analysis of RM-1 cells using anti-UFM1 antibody (A05543-1). Overlay histogram showing RM-1 cells stained with A05543-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UFM1 Antibody (A05543-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324922026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05921-1-peli1-primary-antibodies-wb-testing-1.jpgAnti-PELI1 Antibody Picoband®Western blot analysis of PELI1 using anti-PELI1 antibody (A05921-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PELI1 antigen affinity purified polyclonal antibody (A05921-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PELI1 at approximately 40 kDa. The expected band size for PELI1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05921-1-peli1-primary-antibodies-ihc-testing-1.jpgAnti-PELI1 Antibody Picoband®IHC analysis of PELI1 using anti-PELI1 antibody (A05921-1). PELI1 was detected in a paraffin-embedded section of human skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PELI1 Antibody (A05921-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05921-1-peli1-primary-antibodies-ihc-testing-2.jpgAnti-PELI1 Antibody Picoband®IHC analysis of PELI1 using anti-PELI1 antibody (A05921-1). PELI1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PELI1 Antibody (A05921-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05921-1-peli1-primary-antibodies-ihc-testing-3.jpgAnti-PELI1 Antibody Picoband®IHC analysis of PELI1 using anti-PELI1 antibody (A05921-1). PELI1 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PELI1 Antibody (A05921-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05921-1-peli1-primary-antibodies-ip-testing-1.jpgAnti-PELI1 Antibody Picoband®Immunoprecipitating PELI1 in Caco-2 whole cell lysate. Western blot analysis of PELI1 using anti-PELI1 antibody (A05921-1). Lane 1: Caco-2 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-PELI1 antibody in Caco-2 whole cell lysate, Lane 3: anti-PELI1 antibody (2μg) + Caco-2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PELI1 antigen affinity purified polyclonal antibody (A05921-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PELI1 at approximately 40 kDa. The expected band size for PELI1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05921-1-peli1-primary-antibodies-fcm-testing-1.jpgAnti-PELI1 Antibody Picoband®Flow Cytometry analysis of PC-12 cells using anti-PELI1 antibody (A05921-1). Overlay histogram showing PC-12 cells stained with A05921-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PELI1 Antibody (A05921-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05921-1-peli1-primary-antibodies-fcm-testing-2.jpgAnti-PELI1 Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-PELI1 antibody (A05921-1). Overlay histogram showing THP-1 cells stained with A05921-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PELI1 Antibody (A05921-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324932026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08728-1-pex5l-primary-antibodies-wb-testing-1.jpgAnti-PEX5L Antibody Picoband®Western blot analysis of PEX5L using anti-PEX5L antibody (A08728-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEX5L antigen affinity purified polyclonal antibody (A08728-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PEX5L at approximately 65 kDa. The expected band size for PEX5L is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08728-1-pex5l-primary-antibodies-ihc-testing-1.jpgAnti-PEX5L Antibody Picoband®IHC analysis of PEX5L using anti-PEX5L antibody (A08728-1). PEX5L was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PEX5L Antibody (A08728-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08728-1-pex5l-primary-antibodies-ihc-testing-2.jpgAnti-PEX5L Antibody Picoband®IHC analysis of PEX5L using anti-PEX5L antibody (A08728-1). PEX5L was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PEX5L Antibody (A08728-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08728-1-pex5l-primary-antibodies-ihc-testing-3.jpgAnti-PEX5L Antibody Picoband®IHC analysis of PEX5L using anti-PEX5L antibody (A08728-1). PEX5L was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PEX5L Antibody (A08728-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08728-1-pex5l-primary-antibodies-ihc-testing-4.jpgAnti-PEX5L Antibody Picoband®IHC analysis of PEX5L using anti-PEX5L antibody (A08728-1). PEX5L was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PEX5L Antibody (A08728-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08728-1-pex5l-primary-antibodies-if-testing-1.jpgAnti-PEX5L Antibody Picoband®IF analysis of PEX5L using anti-PEX5L antibody (A08728-1). PEX5L was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PEX5L Antibody (A08728-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08728-1-pex5l-primary-antibodies-if-testing-2.jpgAnti-PEX5L Antibody Picoband®IF analysis of PEX5L using anti-PEX5L antibody (A08728-1). PEX5L was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PEX5L Antibody (A08728-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08728-1-pex5l-primary-antibodies-fcm-testing-1.jpgAnti-PEX5L Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-PEX5L antibody (A08728-1). Overlay histogram showing U251 cells stained with A08728-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PEX5L Antibody (A08728-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324942026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06447-2-pfkl-primary-antibodies-wb-testing-1.jpgAnti-PFKL Antibody Picoband®Western blot analysis of PFKL using anti-PFKL antibody (A06447-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKL antigen affinity purified polyclonal antibody (A06447-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PFKL at approximately 85 kDa. The expected band size for PFKL is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06447-2-pfkl-primary-antibodies-if-testing-1.jpgAnti-PFKL Antibody Picoband®IF analysis of PFKL using anti-PFKL antibody (A06447-2). PFKL was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PFKL Antibody (A06447-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1324952026-03-17T05:17:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05914-2-pglyrp1-primary-antibodies-wb-testing-1.jpgAnti-PGLYRP1 Antibody Picoband®Western blot analysis of PGLYRP1 using anti-PGLYRP1 antibody (A05914-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat UMR-106 whole cell lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse MH-S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGLYRP1 antigen affinity purified polyclonal antibody (A05914-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PGLYRP1 at approximately 30 kDa. The expected band size for PGLYRP1 is at 22 kDa. https://www.bosterbio.com/catalog/product/view/id/1324962026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06531-1-pgrmc2-primary-antibodies-wb-testing-1.jpgAnti-PGRMC2 Antibody Picoband®Western blot analysis of PGRMC2 using anti-PGRMC2 antibody (A06531-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGRMC2 antigen affinity purified polyclonal antibody (A06531-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PGRMC2 at approximately 24-28 kDa. The expected band size for PGRMC2 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06531-1-pgrmc2-primary-antibodies-ihc-testing-1.jpgAnti-PGRMC2 Antibody Picoband®IHC analysis of PGRMC2 using anti-PGRMC2 antibody (A06531-1). PGRMC2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGRMC2 Antibody (A06531-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06531-1-pgrmc2-primary-antibodies-ihc-testing-2.jpgAnti-PGRMC2 Antibody Picoband®IHC analysis of PGRMC2 using anti-PGRMC2 antibody (A06531-1). PGRMC2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGRMC2 Antibody (A06531-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06531-1-pgrmc2-primary-antibodies-if-testing-1.jpgAnti-PGRMC2 Antibody Picoband®IF analysis of PGRMC2 using anti-PGRMC2 antibody (A06531-1). PGRMC2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PGRMC2 Antibody (A06531-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06531-1-pgrmc2-primary-antibodies-if-testing-2.jpgAnti-PGRMC2 Antibody Picoband®IF analysis of PGRMC2 using anti-PGRMC2 antibody (A06531-1). PGRMC2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PGRMC2 Antibody (A06531-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1324972026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09961-1-phf23-primary-antibodies-wb-testing-1.jpgAnti-PHF23 Antibody Picoband®Western blot analysis of PHF23 using anti-PHF23 antibody (A09961-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF23 antigen affinity purified polyclonal antibody (A09961-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHF23 at approximately 65 kDa. The expected band size for PHF23 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09961-1-phf23-primary-antibodies-ihc-testing-1.jpgAnti-PHF23 Antibody Picoband®IHC analysis of PHF23 using anti-PHF23 antibody (A09961-1). PHF23 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PHF23 Antibody (A09961-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09961-1-phf23-primary-antibodies-ihc-testing-2.jpgAnti-PHF23 Antibody Picoband®IHC analysis of PHF23 using anti-PHF23 antibody (A09961-1). PHF23 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PHF23 Antibody (A09961-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09961-1-phf23-primary-antibodies-ihc-testing-3.jpgAnti-PHF23 Antibody Picoband®IHC analysis of PHF23 using anti-PHF23 antibody (A09961-1). PHF23 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PHF23 Antibody (A09961-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09961-1-phf23-primary-antibodies-if-testing-1.jpgAnti-PHF23 Antibody Picoband®IF analysis of PHF23 using anti-PHF23 antibody (A09961-1). PHF23 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PHF23 Antibody (A09961-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09961-1-phf23-primary-antibodies-if-testing-2.jpgAnti-PHF23 Antibody Picoband®IF analysis of PHF23 using anti-PHF23 antibody (A09961-1). PHF23 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PHF23 Antibody (A09961-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09961-1-phf23-primary-antibodies-fcm-testing-1.jpgAnti-PHF23 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-PHF23 antibody (A09961-1). Overlay histogram showing 293T cells stained with A09961-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHF23 Antibody (A09961-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1324982026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04912-3-phlda2-primary-antibodies-wb-testing-1.jpgAnti-PHLDA2 Antibody Picoband®Western blot analysis of PHLDA2 using anti-PHLDA2 antibody (A04912-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHLDA2 antigen affinity purified polyclonal antibody (A04912-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHLDA2 at approximately 20-25 kDa. The expected band size for PHLDA2 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04912-3-phlda2-primary-antibodies-if-testing-1.jpgAnti-PHLDA2 Antibody Picoband®IF analysis of PHLDA2 using anti-PHLDA2 antibody (A04912-3) and anti-Alpha Tubulin antibody (M03989-3). PHLDA2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PHLDA2 Antibody (A04912-3) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1324992026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09834-1-pglyrp4-primary-antibodies-wb-testing-1.jpgAnti-PGLYRP4 Antibody Picoband®Western blot analysis of PGLYRP4 using anti-PGLYRP4 antibody (A09834-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGLYRP4 antigen affinity purified polyclonal antibody (A09834-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PGLYRP4 at approximately 46 kDa. The expected band size for PGLYRP4 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09834-1-pglyrp4-primary-antibodies-fcm-testing-1.jpgAnti-PGLYRP4 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-PGLYRP4 antibody (A09834-1). Overlay histogram showing K562 cells stained with A09834-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PGLYRP4 Antibody (A09834-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1325002026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06468-1-fgfbp1-primary-antibodies-wb-testing-1.jpgAnti-FGFBP1 Antibody Picoband®Western blot analysis of FGFBP1 using anti-FGFBP1 antibody (A06468-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFBP1 antigen affinity purified polyclonal antibody (A06468-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FGFBP1 at approximately 26 kDa. The expected band size for FGFBP1 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06468-1-fgfbp1-primary-antibodies-ihc-testing-1.jpgAnti-FGFBP1 Antibody Picoband®IHC analysis of FGFBP1 using anti-FGFBP1 antibody (A06468-1). FGFBP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGFBP1 Antibody (A06468-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06468-1-fgfbp1-primary-antibodies-fcm-testing-1.jpgAnti-FGFBP1 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-FGFBP1 antibody (A06468-1). Overlay histogram showing PC-3 cells stained with A06468-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-FGFBP1 Antibody (A06468-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1325012026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04986-3-fez1-primary-antibodies-wb-testing-1.jpgAnti-FEZ1 Antibody Picoband®Western blot analysis of FEZ1 using anti-FEZ1 antibody (A04986-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FEZ1 antigen affinity purified polyclonal antibody (A04986-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FEZ1 at approximately 65 kDa. The expected band size for FEZ1 is at 45 kDa. https://www.bosterbio.com/catalog/product/view/id/1325022026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09690-1-phf12-primary-antibodies-wb-testing-1.jpgAnti-PHF12 Antibody Picoband®Western blot analysis of PHF12 using anti-PHF12 antibody (A09690-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF12 antigen affinity purified polyclonal antibody (A09690-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHF12 at approximately 120 kDa. The expected band size for PHF12 is at 120 kDa. https://www.bosterbio.com/catalog/product/view/id/1325032026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04110-1-phlpp2-primary-antibodies-wb-testing-1.jpgAnti-PHLPP2 Antibody Picoband®Western blot analysis of PHLPP2 using anti-PHLPP2 antibody (A04110-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHLPP2 antigen affinity purified polyclonal antibody (A04110-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHLPP2 at approximately 147 kDa. The expected band size for PHLPP2 is at 147 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tph2-antibody-aza0a8m1p7e6-boster.html2026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1p7e6-tph2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish TPH2 AntibodyIHC analysis of TPH2 using anti-TPH2 antibody (AZA0A8M1P7E6). TPH2 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TPH2 Antibody (AZA0A8M1P7E6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-irf7-picoband-antibody-azf1qng2-boster.html2026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qng2-irf7-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish IRF7 Antibody Picoband®Western blot analysis of IRF7 using anti-IRF7 antibody (AZF1QNG2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF7 antigen affinity purified polyclonal antibody (AZF1QNG2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IRF7 at approximately 48 kDa. The expected band size for IRF7 is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pr-pgr-picoband-antibody-azc9v3n7-boster.html2026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azc9v3n7-pgr-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PR/PGR Antibody Picoband®Western blot analysis of PGR using anti-PGR antibody (AZC9V3N7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGR antigen affinity purified polyclonal antibody (AZC9V3N7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PGR at approximately 100 kDa. The expected band size for PGR is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hsd17b1-antibody-azf1qup2-boster.html2026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qup2-hsd17b1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HSD17B1 AntibodyIHC analysis of HSD17B1 using anti-HSD17B1 antibody (AZF1QUP2). HSD17B1 was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD17B1 Antibody (AZF1QUP2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qup2-hsd17b1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HSD17B1 AntibodyIHC analysis of HSD17B1 using anti-HSD17B1 antibody (AZF1QUP2). HSD17B1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD17B1 Antibody (AZF1QUP2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-slc6a4a-picoband-antibody-azq1wgb5-boster.html2026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1wgb5-slc6a4a-primary-antibodies-wb-testing-1_1.jpgAnti-Zebrafish SLC6A4A Antibody Picoband®Western blot analysis of SLC6A4A using anti-SLC6A4A antibody (AZQ1WGB5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A4A antigen affinity purified polyclonal antibody (AZQ1WGB5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC6A4A at approximately 90-95 kDa. The expected band size for SLC6A4A is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1wgb5-slc6a4a-primary-antibodies-ihc-testing-1_1.jpgAnti-Zebrafish SLC6A4A Antibody Picoband®IHC analysis of SLC6A4A using anti-SLC6A4A antibody (AZQ1WGB5). SLC6A4A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC6A4A Antibody (AZQ1WGB5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1wgb5-slc6a4a-primary-antibodies-ihc-testing-2_1.jpgAnti-Zebrafish SLC6A4A Antibody Picoband®IHC analysis of SLC6A4A using anti-SLC6A4A antibody (AZQ1WGB5). SLC6A4A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC6A4A Antibody (AZQ1WGB5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ccn2a-picoband-antibody-azq5ri33-boster.html2026-03-17T05:17:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5ri33-ccn2a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CCN2A Antibody Picoband®Western blot analysis of CCN2A using anti-CCN2A antibody (AZQ5RI33). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCN2A antigen affinity purified polyclonal antibody (AZQ5RI33) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CCN2A at approximately 38 kDa. The expected band size for CCN2A is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ccng1-picoband-antibody-azq7ztx1-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7ztx1-ccng1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CCNG1 Antibody Picoband®Western blot analysis of CCNG1 using anti-CCNG1 antibody (AZQ7ZTX1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNG1 antigen affinity purified polyclonal antibody (AZQ7ZTX1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CCNG1 at approximately 34 kDa. The expected band size for CCNG1 is at 18 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gad65-gad2-picoband-antibody-azf1r9e8-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r9e8-gad2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GAD65/GAD2 Antibody Picoband®Western blot analysis of GAD2 using anti-GAD2 antibody (AZF1R9E8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD2 antigen affinity purified polyclonal antibody (AZF1R9E8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GAD2 at approximately 65 kDa. The expected band size for GAD2 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r9e8-gad2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish GAD65/GAD2 Antibody Picoband®IHC analysis of GAD2 using anti-GAD2 antibody (AZF1R9E8). GAD2 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GAD2 Antibody (AZF1R9E8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nfkbiaa-picoband-antibody-azf1qcg4-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qcg4-nfkbiaa-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NFKBIAA Antibody Picoband®Western blot analysis of NFKBIAA using anti-NFKBIAA antibody (AZF1QCG4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFKBIAA antigen affinity purified polyclonal antibody (AZF1QCG4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NFKBIAA at approximately 35 kDa. The expected band size for NFKBIAA is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qcg4-nfkbiaa-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish NFKBIAA Antibody Picoband®IHC analysis of NFKBIAA using anti-NFKBIAA antibody (AZF1QCG4). NFKBIAA was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NFKBIAA Antibody (AZF1QCG4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-foxj1a-antibody-azf1qdf8-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qdf8-foxj1a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish FOXJ1A AntibodyIHC analysis of FOXJ1A using anti-FOXJ1A antibody (AZF1QDF8). FOXJ1A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXJ1A Antibody (AZF1QDF8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qdf8-foxj1a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish FOXJ1A AntibodyIHC analysis of FOXJ1A using anti-FOXJ1A antibody (AZF1QDF8). FOXJ1A was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXJ1A Antibody (AZF1QDF8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qdf8-foxj1a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish FOXJ1A AntibodyIHC analysis of FOXJ1A using anti-FOXJ1A antibody (AZF1QDF8). FOXJ1A was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXJ1A Antibody (AZF1QDF8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hbae3-picoband-antibody-azq7zyz4-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zyz4-hbae3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HBAE3 Antibody Picoband®Western blot analysis of HBAE3 using anti-HBAE3 antibody (AZQ7ZYZ4). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBAE3 antigen affinity purified polyclonal antibody (AZQ7ZYZ4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HBAE3 at approximately 16 kDa. The expected band size for HBAE3 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zyz4-hbae3-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HBAE3 Antibody Picoband®IHC analysis of HBAE3 using anti-HBAE3 antibody (AZQ7ZYZ4). HBAE3 was detected in a paraffin-embedded section of zebrafish erythrocytes tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HBAE3 Antibody (AZQ7ZYZ4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-bmp2a-picoband-antibody-azo13109-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo13109-bmp2a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish BMP2A Antibody Picoband®Western blot analysis of BMP2A using anti-BMP2A antibody (AZO13109). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP2A antigen affinity purified polyclonal antibody (AZO13109) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BMP2A at approximately 45 kDa. The expected band size for BMP2A is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-il4-picoband-antibody-azd1ysm1-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azd1ysm1-il4-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish IL4 Antibody Picoband®Western blot analysis of IL4 using anti-IL4 antibody (AZD1YSM1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL4 antigen affinity purified polyclonal antibody (AZD1YSM1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL4 at approximately 14 kDa. The expected band size for IL4 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azd1ysm1-il4-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish IL4 Antibody Picoband®IHC analysis of IL4 using anti-IL4 antibody (AZD1YSM1). IL4 was detected in a paraffin-embedded section of zebrafish bone tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL4 Antibody (AZD1YSM1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azd1ysm1-il4-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish IL4 Antibody Picoband®IHC analysis of IL4 using anti-IL4 antibody (AZD1YSM1). IL4 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL4 Antibody (AZD1YSM1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azd1ysm1-il4-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish IL4 Antibody Picoband®IHC analysis of IL4 using anti-IL4 antibody (AZD1YSM1). IL4 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL4 Antibody (AZD1YSM1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nqo1-picoband-antibody-aza0a2r8pw65-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8pw65-nqo1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NQO1 Antibody Picoband®Western blot analysis of NQO1 using anti-NQO1 antibody (AZA0A2R8PW65). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO1 antigen affinity purified polyclonal antibody (AZA0A2R8PW65) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NQO1 at approximately 30 kDa. The expected band size for NQO1 is at 30 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-alas2-picoband-antibody-azq9yht4-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9yht4-alas2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ALAS2 Antibody Picoband®Western blot analysis of ALAS2 using anti-ALAS2 antibody (AZQ9YHT4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALAS2 antigen affinity purified polyclonal antibody (AZQ9YHT4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALAS2 at approximately 50 kDa. The expected band size for ALAS2 is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sst2-antibody-azq9dde4-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9dde4-sst2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish SST2 AntibodyIHC analysis of SST2 using anti-SST2 antibody (AZQ9DDE4). SST2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SST2 Antibody (AZQ9DDE4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-bcl-xl-bcl2l1-antibody-azq90z98-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90z98-bcl2l1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Bcl-XL/BCL2L1 AntibodyIHC analysis of BCL2L1 using anti-BCL2L1 antibody (AZQ90Z98). BCL2L1 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCL2L1 Antibody (AZQ90Z98) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-irf3-picoband-antibody-aza0a8m6z9b8-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6z9b8-irf3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish IRF3 Antibody Picoband®Western blot analysis of IRF3 using anti-IRF3 antibody (AZA0A8M6Z9B8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF3 antigen affinity purified polyclonal antibody (AZA0A8M6Z9B8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IRF3 at approximately 55 kDa. The expected band size for IRF3 is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ifnphi3-picoband-antibody-aza8e6e3-boster.html2026-03-17T05:17:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8e6e3-ifnphi3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish IFNPHI3 Antibody Picoband®Western blot analysis of IFNPHI3 using anti-IFNPHI3 antibody (AZA8E6E3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNPHI3 antigen affinity purified polyclonal antibody (AZA8E6E3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IFNPHI3 at approximately 21 kDa. The expected band size for IFNPHI3 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8e6e3-ifnphi3-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish IFNPHI3 Antibody Picoband®IHC analysis of IFNPHI3 using anti-IFNPHI3 antibody (AZA8E6E3). IFNPHI3 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IFNPHI3 Antibody (AZA8E6E3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mao-picoband-antibody-azq6nsn2-boster.html2026-03-17T05:17:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nsn2-mao-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MAO Antibody Picoband®Western blot analysis of MAO using anti-MAO antibody (AZQ6NSN2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAO antigen affinity purified polyclonal antibody (AZQ6NSN2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAO at approximately 59 kDa. The expected band size for MAO is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nsn2-mao-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish MAO Antibody Picoband®IHC analysis of MAO using anti-MAO antibody (AZQ6NSN2). MAO was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAO Antibody (AZQ6NSN2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fgf20a-antibody-azq38pl0-boster.html2026-03-17T05:17:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq38pl0-fgf20a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish FGF20A AntibodyIHC analysis of FGF20A using anti-FGF20A antibody (AZQ38PL0). FGF20A was detected in a paraffin-embedded section of zebrafish bone tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF20A Antibody (AZQ38PL0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq38pl0-fgf20a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish FGF20A AntibodyIHC analysis of FGF20A using anti-FGF20A antibody (AZQ38PL0). FGF20A was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF20A Antibody (AZQ38PL0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq38pl0-fgf20a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish FGF20A AntibodyIHC analysis of FGF20A using anti-FGF20A antibody (AZQ38PL0). FGF20A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF20A Antibody (AZQ38PL0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ackr3a-picoband-antibody-aza0a2r8pyr8-boster.html2026-03-17T05:17:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8pyr8-ackr3a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ACKR3A Antibody Picoband®Western blot analysis of ACKR3A using anti-ACKR3A antibody (AZA0A2R8PYR8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACKR3A antigen affinity purified polyclonal antibody (AZA0A2R8PYR8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ACKR3A at approximately 40 kDa. The expected band size for ACKR3A is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a2r8pyr8-ackr3a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish ACKR3A Antibody Picoband®IHC analysis of ACKR3A using anti-ACKR3A antibody (AZA0A2R8PYR8). ACKR3A was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACKR3A Antibody (AZA0A2R8PYR8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gadd45aa-picoband-antibody-azf1r8z0-boster.html2026-03-17T05:17:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r8z0-gadd45aa-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GADD45AA Antibody Picoband®Western blot analysis of GADD45AA using anti-GADD45AA antibody (AZF1R8Z0). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45AA antigen affinity purified polyclonal antibody (AZF1R8Z0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GADD45AA at approximately 15 kDa. The expected band size for GADD45AA is at 17 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pdgfrb-picoband-antibody-azd9mna7-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azd9mna7-pdgfrb-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PDGFRB Antibody Picoband®Western blot analysis of PDGFRB using anti-PDGFRB antibody (AZD9MNA7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRB antigen affinity purified polyclonal antibody (AZD9MNA7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDGFRB at approximately 120 kDa. The expected band size for PDGFRB is at 120 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pdgfr-alpha-pdgfra-antibody-azq9de49-boster.html2026-03-17T05:17:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9de49-pdgfra-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish PDGFR alpha/PDGFRA AntibodyIHC analysis of PDGFRA using anti-PDGFRA antibody (AZQ9DE49). PDGFRA was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDGFRA Antibody (AZQ9DE49) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9de49-pdgfra-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish PDGFR alpha/PDGFRA AntibodyIHC analysis of PDGFRA using anti-PDGFRA antibody (AZQ9DE49). PDGFRA was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PDGFRA Antibody (AZQ9DE49) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tbx1-antibody-azq8axx2-boster.html2026-03-17T05:17:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8axx2-tbx1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish TBX1 AntibodyIHC analysis of TBX1 using anti-TBX1 antibody (AZQ8AXX2). TBX1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX1 Antibody (AZQ8AXX2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8axx2-tbx1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish TBX1 AntibodyIHC analysis of TBX1 using anti-TBX1 antibody (AZQ8AXX2). TBX1 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX1 Antibody (AZQ8AXX2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8axx2-tbx1-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish TBX1 AntibodyIHC analysis of TBX1 using anti-TBX1 antibody (AZQ8AXX2). TBX1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TBX1 Antibody (AZQ8AXX2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mstna-picoband-antibody-azq68in2-boster.html2026-03-17T05:17:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq68in2-mstna-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MSTNA Antibody Picoband®Western blot analysis of MSTNA using anti-MSTNA antibody (AZQ68IN2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSTNA antigen affinity purified polyclonal antibody (AZQ68IN2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MSTNA at approximately 41 kDa. The expected band size for MSTNA is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq68in2-mstna-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish MSTNA Antibody Picoband®IHC analysis of MSTNA using anti-MSTNA antibody (AZQ68IN2). MSTNA was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSTNA Antibody (AZQ68IN2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq68in2-mstna-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish MSTNA Antibody Picoband®IHC analysis of MSTNA using anti-MSTNA antibody (AZQ68IN2). MSTNA was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSTNA Antibody (AZQ68IN2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq68in2-mstna-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish MSTNA Antibody Picoband®IHC analysis of MSTNA using anti-MSTNA antibody (AZQ68IN2). MSTNA was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSTNA Antibody (AZQ68IN2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq68in2-mstna-primary-antibodies-ihc-testing-4.jpgAnti-Zebrafish MSTNA Antibody Picoband®IHC analysis of MSTNA using anti-MSTNA antibody (AZQ68IN2). MSTNA was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSTNA Antibody (AZQ68IN2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-slc4a1a-picoband-antibody-azq7zzj7-boster.html2026-03-17T05:17:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zzj7-slc4a1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SLC4A1A Antibody Picoband®Western blot analysis of SLC4A1A using anti-SLC4A1A antibody (AZQ7ZZJ7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC4A1A antigen affinity purified polyclonal antibody (AZQ7ZZJ7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC4A1A at approximately 101 kDa. The expected band size for SLC4A1A is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zzj7-slc4a1a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish SLC4A1A Antibody Picoband®IHC analysis of SLC4A1A using anti-SLC4A1A antibody (AZQ7ZZJ7). SLC4A1A was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC4A1A Antibody (AZQ7ZZJ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zzj7-slc4a1a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish SLC4A1A Antibody Picoband®IHC analysis of SLC4A1A using anti-SLC4A1A antibody (AZQ7ZZJ7). SLC4A1A was detected in a paraffin-embedded section of zebrafish spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC4A1A Antibody (AZQ7ZZJ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zzj7-slc4a1a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish SLC4A1A Antibody Picoband®IHC analysis of SLC4A1A using anti-SLC4A1A antibody (AZQ7ZZJ7). SLC4A1A was detected in a paraffin-embedded section of zebrafish gills tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC4A1A Antibody (AZQ7ZZJ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-foxc1a-antibody-azq9de25-boster.html2026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9de25-foxc1a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish FOXC1A AntibodyIHC analysis of FOXC1A using anti-FOXC1A antibody (AZQ9DE25). FOXC1A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXC1A Antibody (AZQ9DE25) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-myl7-picoband-antibody-azq801m3-boster.html2026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq801m3-myl7-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MYL7 Antibody Picoband®Western blot analysis of MYL7 using anti-MYL7 antibody (AZQ801M3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL7 antigen affinity purified polyclonal antibody (AZQ801M3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYL7 at approximately 17 kDa. The expected band size for MYL7 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq801m3-myl7-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish MYL7 Antibody Picoband®IHC analysis of MYL7 using anti-MYL7 antibody (AZQ801M3). MYL7 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYL7 Antibody (AZQ801M3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq801m3-myl7-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish MYL7 Antibody Picoband®IHC analysis of MYL7 using anti-MYL7 antibody (AZQ801M3). MYL7 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYL7 Antibody (AZQ801M3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-prdx1-picoband-antibody-aza2awe1-boster.html2026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2awe1-prdx1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PRDX1 Antibody Picoband®Western blot analysis of PRDX1 using anti-PRDX1 antibody (AZA2AWE1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX1 antigen affinity purified polyclonal antibody (AZA2AWE1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRDX1 at approximately 22 kDa. The expected band size for PRDX1 is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ttn-antibody-aza0ab32tzz1-boster.html2026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0ab32tzz1-ttn-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish TTN AntibodyIHC analysis of TTN using anti-TTN antibody (AZA0AB32TZZ1). TTN was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTN Antibody (AZA0AB32TZZ1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0ab32tzz1-ttn-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish TTN AntibodyIHC analysis of TTN using anti-TTN antibody (AZA0AB32TZZ1). TTN was detected in a paraffin-embedded section of zebrafish bone tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TTN Antibody (AZA0AB32TZZ1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cd326-epcam-picoband-antibody-azq568h0-boster.html2026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq568h0-epcam-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CD326/EPCAM Antibody Picoband®Western blot analysis of EPCAM using anti-EPCAM antibody (AZQ568H0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPCAM antigen affinity purified polyclonal antibody (AZQ568H0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPCAM at approximately 40 kDa. The expected band size for EPCAM is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq568h0-epcam-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish CD326/EPCAM Antibody Picoband®IHC analysis of EPCAM using anti-EPCAM antibody (AZQ568H0). EPCAM was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPCAM Antibody (AZQ568H0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq568h0-epcam-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish CD326/EPCAM Antibody Picoband®IHC analysis of EPCAM using anti-EPCAM antibody (AZQ568H0). EPCAM was detected in a paraffin-embedded section of zebrafish esophagus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPCAM Antibody (AZQ568H0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq568h0-epcam-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish CD326/EPCAM Antibody Picoband®IHC analysis of EPCAM using anti-EPCAM antibody (AZQ568H0). EPCAM was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPCAM Antibody (AZQ568H0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cytokeratin-8-krt8-picoband-antibody-azq6nwf6-boster.html2026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nwf6-krt8-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Cytokeratin 8/KRT8 Antibody Picoband®Western blot analysis of KRT8 using anti-KRT8 antibody (AZQ6NWF6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT8 antigen affinity purified polyclonal antibody (AZQ6NWF6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KRT8 at approximately 57 kDa. The expected band size for KRT8 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nwf6-krt8-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Cytokeratin 8/KRT8 Antibody Picoband®IHC analysis of KRT8 using anti-KRT8 antibody (AZQ6NWF6). KRT8 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KRT8 Antibody (AZQ6NWF6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nwf6-krt8-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Cytokeratin 8/KRT8 Antibody Picoband®IHC analysis of KRT8 using anti-KRT8 antibody (AZQ6NWF6). KRT8 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KRT8 Antibody (AZQ6NWF6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nwf6-krt8-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish Cytokeratin 8/KRT8 Antibody Picoband®IHC analysis of KRT8 using anti-KRT8 antibody (AZQ6NWF6). KRT8 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KRT8 Antibody (AZQ6NWF6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lhx3-picoband-antibody-azq90421-boster.html2026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90421-lhx3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LHX3 Antibody Picoband®Western blot analysis of LHX3 using anti-LHX3 antibody (AZQ90421). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LHX3 antigen affinity purified polyclonal antibody (AZQ90421) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LHX3 at approximately 50 kDa. The expected band size for LHX3 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90421-lhx3-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish LHX3 Antibody Picoband®IHC analysis of LHX3 using anti-LHX3 antibody (AZQ90421). LHX3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHX3 Antibody (AZQ90421) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90421-lhx3-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish LHX3 Antibody Picoband®IHC analysis of LHX3 using anti-LHX3 antibody (AZQ90421). LHX3 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHX3 Antibody (AZQ90421) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hdac1-antibody-clone-31h07-m00256-3-boster.html2026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-oct4-pou5f1-antibody-clone-31p08-m00174-3-boster.html2026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sirt3-antibody-clone-31s09-m01061-boster.html2026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01061-sirt3-primary-antibodies-wb-testing-1.jpgAnti-SIRT3 Antibody (Monoclonal, 31S09)Western blot analysis of SIRT3 using anti-SIRT3 antibody (M01061). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates Lane 7: mouse kidney tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT3 antigen affinity purified monoclonal antibody (M01061) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SIRT3 at approximately 28 kDa. The expected band size for SIRT3 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01061-sirt3-primary-antibodies-wb-testing-2.jpgAnti-SIRT3 Antibody (Monoclonal, 31S09)Western blot analysis of SIRT3 using anti-SIRT3 antibody (M01061). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT3 antigen affinity purified monoclonal antibody (M01061) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SIRT3 at approximately 28 kDa. The expected band size for SIRT3 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01061-sirt3-primary-antibodies-ihc-testing-1.jpgAnti-SIRT3 Antibody (Monoclonal, 31S09)IHC analysis of SIRT3 using anti-SIRT3 antibody (M01061). SIRT3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SIRT3 Antibody (M01061) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01061-sirt3-primary-antibodies-ihc-testing-2.jpgAnti-SIRT3 Antibody (Monoclonal, 31S09)IHC analysis of SIRT3 using anti-SIRT3 antibody (M01061). SIRT3 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SIRT3 Antibody (M01061) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01061-sirt3-primary-antibodies-ihc-testing-3.jpgAnti-SIRT3 Antibody (Monoclonal, 31S09)IHC analysis of SIRT3 using anti-SIRT3 antibody (M01061). SIRT3 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SIRT3 Antibody (M01061) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01061-sirt3-primary-antibodies-ihc-testing-4.jpgAnti-SIRT3 Antibody (Monoclonal, 31S09)IHC analysis of SIRT3 using anti-SIRT3 antibody (M01061). SIRT3 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SIRT3 Antibody (M01061) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gap43-antibody-clone-31g10-m01868-6-boster.html2026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd81-antibody-clone-31c11-m01281-2-boster.html2026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trim21-antibody-clone-31t12-m02079-boster.html2026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-transketolase-tkt-antibody-clone-31t13-m02197-3-boster.html2026-03-16T05:10:42+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gli1-antibody-clone-31g14-m00527-3-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chchd2-antibody-clone-31c15-m06266-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ddx4-antibody-clone-31d16-m02448-3-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p150-glued-dctn1-antibody-clone-31d17-m02175-2-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-irf5-antibody-clone-31i18-m00958-4-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smurf1-antibody-clone-31s19-m02823-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mkp-1-dusp1-antibody-clone-31d20-m02276-1-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rxrb-antibody-clone-31r21-m05488-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dlx2-antibody-clone-31d22-m03617-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slc2a4-antibody-clone-31s23-m00528-1-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sdf-1-cxcl12-antibody-clone-31c24-m00053-2-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-col5a1-antibody-clone-31c25-m02283-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-twist1-antibody-clone-31t26-m00980-2-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tfeb-antibody-clone-31t27-m00662-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tcf7-antibody-clone-31t28-m01315-boster.html2026-03-16T05:10:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01315-tcf7-primary-antibodies-wb-testing-1.jpgAnti-TCF7 Antibody (Monoclonal, 31T28)Western blot analysis of TCF7 using anti-TCF7 antibody (M01315). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCF7 antigen affinity purified monoclonal antibody (M01315) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TCF7 at approximately 42 kDa. The expected band size for TCF7 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ccs-antibody-clone-31c29-m00314-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mapkapk5-antibody-clone-31m30-m07923-1-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bik-antibody-clone-31b31-m03188-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-klrbc-antibody-clone-31k32-m30376-boster.html2026-03-16T05:10:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sec61b-antibody-clone-31s33-m07334-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-glut2-slc2a2-antibody-clone-31s34-m02297-2-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hba1-antibody-clone-31h35-m00233-1-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-foxp3-antibody-clone-31f36-m00011-6-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-epo-antibody-clone-31e37-m00484-1-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-decorin-dcn-antibody-clone-31d38-m01339-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tmem119-antibody-clone-31t39-m09325-boster.html2026-03-16T05:10:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09325-tmem119-primary-antibodies-wb-testing-1.jpgAnti-TMEM119 Antibody (Monoclonal, 31T39)Western blot analysis of TMEM119 using anti-TMEM119 antibody (M09325). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM119 antigen affinity purified monoclonal antibody (M09325) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMEM119 at approximately 52 kDa. The expected band size for TMEM119 is at 29 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gpi-antibody-clone-31g40-m00108-3-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-batf3-antibody-clone-31b41-m01957-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dlx2-antibody-clone-31d42-m03617-1-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cacna1e-antibody-clone-31c43-m05359-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sgcg-antibody-clone-31s44-m05934-2-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-drd2-antibody-clone-31d45-m00289-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c9orf72-antibody-clone-31c46-m00419-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dr3-tnfrsf25-antibody-clone-31t47-m03227-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dystroglycan-dag1-antibody-clone-31d48-m01369-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cea-ceacam5-antibody-clone-31c49-m00356-11-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-granzyme-a-gzma-antibody-clone-31g50-m02779-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mff-antibody-clone-31m51-m02563-1-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufs1-antibody-clone-31n52-m04920-1-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p28-il27-antibody-clone-31i53-m00857-2-boster.html2026-03-16T05:10:44+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp5o-atp5po-antibody-clone-31a54-m32274-1-boster.html2026-03-16T05:10:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mul1-antibody-clone-31m55-m05590-boster.html2026-03-16T05:10:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psmb5-antibody-clone-31p56-m03418-boster.html2026-03-16T05:10:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cab39-antibody-clone-31c57-m08197-1-boster.html2026-03-16T05:10:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-top2a-specific-antibody-clone-31t58-m00953-3-boster.html2026-03-16T05:10:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd21-cr2-antibody-clone-31c59-m01632-3-boster.html2026-03-16T05:10:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01632-3-cd21-primary-antibodies-ihc-testing-1.jpgAnti-CD21/CR2 Antibody (Monoclonal, 31C59)IHC analysis of CD21 using anti-CD21 antibody (M01632-3). CD21 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD21 Antibody (M01632-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01632-3-cd21-primary-antibodies-ihc-testing-2.jpgAnti-CD21/CR2 Antibody (Monoclonal, 31C59)IHC analysis of CD21 using anti-CD21 antibody (M01632-3). CD21 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD21 Antibody (M01632-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01632-3-cd21-primary-antibodies-ihc-testing-3.jpgAnti-CD21/CR2 Antibody (Monoclonal, 31C59)IHC analysis of CD21 using anti-CD21 antibody (M01632-3). CD21 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD21 Antibody (M01632-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01632-3-cd21-primary-antibodies-ihc-testing-4.jpgAnti-CD21/CR2 Antibody (Monoclonal, 31C59)IHC analysis of CD21 using anti-CD21 antibody (M01632-3). CD21 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD21 Antibody (M01632-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01632-3-cd21-primary-antibodies-ihc-testing-5.jpgAnti-CD21/CR2 Antibody (Monoclonal, 31C59)IHC analysis of CD21 using anti-CD21 antibody (M01632-3). CD21 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD21 Antibody (M01632-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-e2f2-antibody-clone-31e60-m02896-1-boster.html2026-03-16T05:10:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il18-antibody-clone-31i61-m00124-5-boster.html2026-03-16T05:10:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apolipoprotein-ai-apoa1-antibody-clone-31a62-m00717-2-boster.html2026-03-16T05:10:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-zw10-antibody-clone-31z63-m04266-boster.html2026-03-16T05:10:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slc7a5-antibody-clone-31s64-m01016-2-boster.html2026-03-16T05:10:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01016-2-slc7a5-primary-antibodies-wb-testing-1.jpgAnti-SLC7A5 Antibody (Monoclonal, 31S64)Western blot analysis of SLC7A5 using anti-SLC7A5 antibody (M01016-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC7A5 antigen affinity purified monoclonal antibody (M01016-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC7A5 at approximately 38 kDa. The expected band size for SLC7A5 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01016-2-slc7a5-primary-antibodies-ihc-testing-1.jpgAnti-SLC7A5 Antibody (Monoclonal, 31S64)IHC analysis of SLC7A5 using anti-SLC7A5 antibody (M01016-2). SLC7A5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SLC7A5 Antibody (M01016-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01016-2-slc7a5-primary-antibodies-ihc-testing-2.jpgAnti-SLC7A5 Antibody (Monoclonal, 31S64)IHC analysis of SLC7A5 using anti-SLC7A5 antibody (M01016-2). SLC7A5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SLC7A5 Antibody (M01016-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trkc-ntrk3-antibody-clone-31n65-m02502-4-boster.html2026-03-16T05:10:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-scd1-antibody-clone-31s66-m32769-boster.html2026-03-16T05:10:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32769-scd1-primary-antibodies-ihc-testing-1.jpgAnti-SCD1 Antibody (Monoclonal, 31S66)IHC analysis of SCD1 using anti-SCD1 antibody (M32769). SCD1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SCD1 Antibody (M32769) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/3/m32769-scd1-primary-antibodies-ihc-testing-2.jpgAnti-SCD1 Antibody (Monoclonal, 31S66)IHC analysis of SCD1 using anti-SCD1 antibody (M32769). SCD1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SCD1 Antibody (M32769) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-asm-smpd1-antibody-clone-31s67-m00752-1-boster.html2026-03-16T05:10:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-klf5-antibody-clone-31k68-m00727-2-boster.html2026-03-16T05:10:45+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-granzyme-k-gzmk-antibody-clone-31g69-m06281-boster.html2026-03-16T05:10:46+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-scnn1g-antibody-clone-31s70-m02366-boster.html2026-03-16T05:10:46+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dnajc15-antibody-clone-31d71-m08200-1-boster.html2026-03-16T05:10:46+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cyp3a4-antibody-clone-31c72-m00339-2-boster.html2026-03-16T05:10:46+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ercc1-antibody-clone-31e73-m00388-3-boster.html2026-03-16T05:10:46+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-m-csf-csf1-antibody-clone-31c74-m00620-3-boster.html2026-03-16T05:10:46+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ibsp-antibody-clone-31i75-m03183-boster.html2026-03-16T05:10:46+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-connexin-43-gja1-antibody-clone-31g76-m00599-boster.html2026-03-16T05:10:46+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tie2-tek-antibody-clone-31t77-m01274-1-boster.html2026-03-16T05:10:47+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ku80-xrcc5-antibody-clone-31x78-m01275-3-boster.html2026-03-16T05:10:47+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psat1-antibody-clone-31p79-m06277-boster.html2026-03-16T05:10:47+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dsc1-antibody-clone-31d80-m03478-1-boster.html2026-03-16T05:10:47+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-esrra-antibody-clone-31e81-m03339-2-boster.html2026-03-16T05:10:47+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hcls1-antibody-clone-31h82-m04313-boster.html2026-03-16T05:10:47+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lats1-antibody-clone-31l83-m01051-boster.html2026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01051-lats1-primary-antibodies-wb-testing-1.jpgAnti-LATS1 Antibody (Monoclonal, 31L83)Western blot analysis of LATS1 using anti-LATS1 antibody (M01051). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LATS1 antigen affinity purified monoclonal antibody (M01051) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LATS1 at approximately 140 kDa. The expected band size for LATS1 is at 127 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mglur5-grm5-antibody-clone-31g84-m01338-2-boster.html2026-03-16T05:10:47+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-flnb-antibody-clone-31f85-m02562-2-boster.html2026-03-16T05:10:47+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tpm2-antibody-clone-31t86-m03082-boster.html2026-03-16T05:10:48+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c16orf84-ctu2-antibody-clone-31c87-m10342-boster.html2026-03-16T05:10:48+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trif-ticam1-antibody-clone-31t88-m01872-1-boster.html2026-03-16T05:10:48+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mx1-antibody-clone-31m89-m00849-1-boster.html2026-03-16T05:10:48+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-limk2-antibody-clone-31l90-m03511-boster.html2026-03-16T05:10:48+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ptpn2-antibody-clone-31p91-m01597-1-boster.html2026-03-16T05:10:48+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tfe3-antibody-clone-31t92-m01791-1-boster.html2026-03-16T05:10:48+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rac2-antibody-clone-31r93-m01714-1-boster.html2026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01714-1-rac2-primary-antibodies-wb-testing-1.jpgAnti-RAC2 Antibody (Monoclonal, 31R93)Western blot analysis of RAC2 using anti-RAC2 antibody (M01714-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAC2 antigen affinity purified monoclonal antibody (M01714-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAC2 at approximately 21 kDa. The expected band size for RAC2 is at 21 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rad51-antibody-clone-31r94-m00088-4-boster.html2026-03-24T05:36:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00088-4-rad51-primary-antibodies-wb-testing-1.jpgAnti-RAD51 Antibody (Monoclonal, 31R94)Western blot analysis of RAD51 using anti-RAD51 antibody (M00088-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51 antigen affinity purified monoclonal antibody (M00088-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAD51 at approximately 38 kDa. The expected band size for RAD51 is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prmt1-antibody-clone-31p95-m01417-2-boster.html2026-03-16T05:10:48+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eaat3-slc1a1-antibody-clone-31s96-m02367-2-boster.html2026-03-16T05:10:48+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gsdmd-antibody-clone-31g97-m02842-2-boster.html2026-03-16T05:10:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02842-2-gsdmd-primary-antibodies-wb-testing-1.jpgAnti-GSDMD Antibody (Monoclonal, 31G97)Western blot analysis of GSDMD using anti-GSDMD antibody (M02842-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSDMD antigen affinity purified monoclonal antibody (M02842-2) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSDMD at approximately 50-53 kDa. The expected band size for GSDMD is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02842-2-gsdmd-primary-antibodies-ihc-testing-1.jpgAnti-GSDMD Antibody (Monoclonal, 31G97)IHC analysis of GSDMD using anti-GSDMD antibody (M02842-2). GSDMD was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-GSDMD Antibody (M02842-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02842-2-gsdmd-primary-antibodies-ihc-testing-2.jpgAnti-GSDMD Antibody (Monoclonal, 31G97)IHC analysis of GSDMD using anti-GSDMD antibody (M02842-2). GSDMD was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-GSDMD Antibody (M02842-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd47-antibody-clone-31c98-m00360-9-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ezh1-antibody-clone-31e99-m02494-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aldh3a1-antibody-clone-32a00-m01121-1-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ent1-slc29a1-antibody-clone-32s01-m02058-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vipr1-antibody-clone-32v02-m04345-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rps6-phospho-s235-s236-antibody-clone-32r03-m01567-2-boster.html2026-03-16T05:10:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01567-2-p-rps6-primary-antibodies-wb-testing-1.jpgAnti-RPS6 (Phospho-S235 + S236) Antibody (Monoclonal, 32R03)Western blot analysis of P-RPS6 using anti-P-RPS6 antibody (M01567-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-RPS6 antigen affinity purified monoclonal antibody (M01567-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-RPS6 at approximately 34 kDa. The expected band size for P-RPS6 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01567-2-p-rps6-primary-antibodies-ihc-testing-1.jpgAnti-RPS6 (Phospho-S235 + S236) Antibody (Monoclonal, 32R03)IHC analysis of P-RPS6 using anti-P-RPS6 antibody (M01567-2). P-RPS6 was detected in a paraffin-embedded section of mouse bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-RPS6 Antibody (M01567-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01567-2-p-rps6-primary-antibodies-ihc-testing-2.jpgAnti-RPS6 (Phospho-S235 + S236) Antibody (Monoclonal, 32R03)IHC analysis of P-RPS6 using anti-P-RPS6 antibody (M01567-2). P-RPS6 was detected in a paraffin-embedded section of rat bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-RPS6 Antibody (M01567-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pdia2-antibody-clone-32p04-m03275-1-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-jagged1-jag1-antibody-clone-32j05-m00640-2-boster.html2026-03-16T05:10:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00640-2-jag1-primary-antibodies-wb-testing-1.jpgAnti-Jagged1/JAG1 Antibody (Monoclonal, 32J05)Western blot analysis of JAG1 using anti-JAG1 antibody (M00640-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAG1 antigen affinity purified monoclonal antibody (M00640-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for JAG1 at approximately 180 kDa. The expected band size for JAG1 is at 134 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00640-2-jag1-primary-antibodies-ihc-testing-1.jpgAnti-Jagged1/JAG1 Antibody (Monoclonal, 32J05)IHC analysis of JAG1 using anti-JAG1 antibody (M00640-2). JAG1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JAG1 Antibody (M00640-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00640-2-jag1-primary-antibodies-ihc-testing-2.jpgAnti-Jagged1/JAG1 Antibody (Monoclonal, 32J05)IHC analysis of JAG1 using anti-JAG1 antibody (M00640-2). JAG1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JAG1 Antibody (M00640-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00640-2-jag1-primary-antibodies-ihc-testing-3.jpgAnti-Jagged1/JAG1 Antibody (Monoclonal, 32J05)IHC analysis of JAG1 using anti-JAG1 antibody (M00640-2). JAG1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JAG1 Antibody (M00640-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-progranulin-grn-antibody-clone-32g06-m00893-3-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psmd13-antibody-clone-32p07-m09882-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fbxo3-antibody-clone-32f08-m08859-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stat4-antibody-clone-32s09-m00734-3-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-plk1-phospho-t210-antibody-clone-32p10-m00182-3-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-krt2-antibody-clone-32k11-m05667-2-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eif3j-antibody-clone-32e12-m06040-boster.html2026-03-16T05:10:49+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cenpf-antibody-clone-32c13-m03311-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-collagen-type-i-col1a2-antibody-clone-32c14-m00624-1-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pancreatic-polypeptide-ppy-antibody-clone-32p15-m01676-1-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hdac10-antibody-clone-32h16-m05215-4-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gch1-antibody-clone-32g17-m01210-1-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd41-integrin-alpha-2b-itga2b-antibody-clone-32i18-m01102-5-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-oxt-antibody-clone-32o19-m01545-1-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-calca-antibody-clone-32c20-m02352-6-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tau-mapt-phospho-s396-antibody-clone-32m21-m00097-11-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chromogranin-a-chga-antibody-clone-32c22-m01256-4-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aqp2-antibody-clone-32a23-m00935-boster.html2026-04-05T05:00:42+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kdm3b-antibody-clone-32k24-m08117-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sparc-antibody-clone-32s25-m00862-2-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fadd-antibody-clone-32f26-m00237-3-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ahnak-antibody-clone-32a27-m05378-1-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smarca2-antibody-clone-32s28-m01888-2-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-olfm4-antibody-clone-32o29-m04094-1-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-snap29-antibody-clone-32s30-m05318-1-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aldolase-c-aldoc-antibody-clone-32a31-m05296-1-boster.html2026-03-16T05:10:50+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eif4a2-antibody-clone-32e32-m06549-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kmt2c-antibody-clone-32k33-m02023-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cnot7-antibody-clone-32c34-m04884-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fgf18-antibody-clone-32f35-m04181-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vtcn1-antibody-clone-32v36-m02821-3-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ikb-alpha-nfkbia-phospho-s36-antibody-clone-32n37-m01139-2-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usp5-antibody-clone-32u38-m04550-4-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-camkk2-antibody-clone-32c39-m03048-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il18-antibody-clone-32i40-m00124-6-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lipt1-antibody-clone-32l41-m11274-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-flcn-antibody-clone-32f42-m00718-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-polm-antibody-clone-32p43-m08812-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-etv5-antibody-clone-32e44-m01809-1-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atl3-antibody-clone-32a45-m09112-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hnrnpa0-antibody-clone-32h46-m09015-1-boster.html2026-03-16T05:10:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09015-1-hnrnpa0-primary-antibodies-wb-testing-1.jpgAnti-HNRNPA0 Antibody (Monoclonal, 32H46)Western blot analysis of HNRNPA0/MRC1 using anti-HNRNPA0/MRC1 antibody (M09015-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human REH whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNRNPA0/MRC1 antigen affinity purified monoclonal antibody (M09015-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HNRNPA0/MRC1 at approximately 34-35 kDa. The expected band size for HNRNPA0/MRC1 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09015-1-hnrnpa0-primary-antibodies-ihc-testing-1.jpgAnti-HNRNPA0 Antibody (Monoclonal, 32H46)IHC analysis of HNRNPA0/MRC1 using anti-HNRNPA0/MRC1 antibody (M09015-1). HNRNPA0/MRC1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-HNRNPA0/MRC1 Antibody (M09015-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09015-1-hnrnpa0-primary-antibodies-ihc-testing-2.jpgAnti-HNRNPA0 Antibody (Monoclonal, 32H46)IHC analysis of HNRNPA0/MRC1 using anti-HNRNPA0/MRC1 antibody (M09015-1). HNRNPA0/MRC1 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-HNRNPA0/MRC1 Antibody (M09015-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09015-1-hnrnpa0-primary-antibodies-ihc-testing-3.jpgAnti-HNRNPA0 Antibody (Monoclonal, 32H46)IHC analysis of HNRNPA0/MRC1 using anti-HNRNPA0/MRC1 antibody (M09015-1). HNRNPA0/MRC1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-HNRNPA0/MRC1 Antibody (M09015-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m09015-1-hnrnpa0-primary-antibodies-ihc-testing-4.jpgAnti-HNRNPA0 Antibody (Monoclonal, 32H46)IHC analysis of HNRNPA0/MRC1 using anti-HNRNPA0/MRC1 antibody (M09015-1). HNRNPA0/MRC1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-HNRNPA0/MRC1 Antibody (M09015-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p53-tp53-antibody-clone-32t47-m00001-8-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-khk-antibody-clone-32k48-m05682-1-boster.html2026-03-16T05:10:51+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cblb-antibody-clone-32c49-m00735-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-carm1-antibody-clone-32c50-m01486-2-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hnrnpab-antibody-clone-32h51-m07146-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cxcr4-antibody-clone-32c52-m00031-4-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sts-antibody-clone-32s53-m01198-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rictor-antibody-clone-32r54-m03195-2-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-depdc6-deptor-antibody-clone-32d55-m03811-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rbbp5-antibody-clone-32r56-m04563-1-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wnt8a-antibody-clone-32w57-m08392-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-card9-antibody-clone-32c58-m01410-1-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p16ink4a-cdkn2a-antibody-clone-32c59-m00016-5-boster.html2026-03-16T05:10:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00016-5-cdkn2a-primary-antibodies-wb-testing-1.jpgAnti-p16INK4a/CDKN2A Antibody (Monoclonal, 32C59)Western blot analysis of CDKN2A using anti-CDKN2A antibody (M00016-5). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDKN2A antigen affinity purified monoclonal antibody (M00016-5) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CDKN2A at approximately 18 kDa. The expected band size for CDKN2A is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00016-5-cdkn2a-primary-antibodies-ihc-testing-1.jpgAnti-p16INK4a/CDKN2A Antibody (Monoclonal, 32C59)IHC analysis of CDKN2A using anti-CDKN2A antibody (M00016-5). CDKN2A was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CDKN2A Antibody (M00016-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vista-vsir-antibody-clone-32v60-m32320-2-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crbn-antibody-clone-32c61-m00983-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-clasp1-antibody-clone-32c62-m04813-2-boster.html2026-03-16T05:10:52+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rab3a-antibody-clone-32r63-m03463-3-boster.html2026-03-16T05:10:53+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-uqcrc1-antibody-clone-32u64-m06974-2-boster.html2026-03-16T05:10:53+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hadha-antibody-clone-32h65-m03666-1-boster.html2026-03-16T05:10:53+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tmp21-tmed10-antibody-clone-32t66-m07234-boster.html2026-03-16T05:10:53+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rabac1-antibody-clone-32r67-m10220-boster.html2026-03-16T05:10:53+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-scp3-sycp3-antibody-clone-32s68-m05718-2-boster.html2026-03-16T05:10:53+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-thrombospondin-1-thbs1-antibody-clone-32t69-m00667-boster.html2026-03-16T05:10:53+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-redd1-ddit4-specific-antibody-clone-32d70-m02019-boster.html2026-03-16T05:10:53+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dlk1-antibody-clone-32d71-m00513-2-boster.html2026-03-16T05:10:53+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fundc1-antibody-clone-32f72-m08688-boster.html2026-03-16T05:10:53+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tom1-antibody-clone-32t73-m04143-boster.html2026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04143-tom1-primary-antibodies-wb-testing-1.jpgAnti-TOM1 Antibody (Monoclonal, 32T73)Western blot analysis of TOM1 using anti-TOM1 antibody (M04143). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOM1 antigen affinity purified monoclonal antibody (M04143) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TOM1 at approximately 54 kDa. The expected band size for TOM1 is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rhoc-antibody-clone-32r74-m01211-1-boster.html2026-03-16T05:10:54+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smc1b-phospho-s937-antibody-clone-32s75-m10280-boster.html2026-03-16T05:10:54+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-scx-antibody-clone-32s76-m04284-boster.html2026-03-16T05:10:54+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tead2-antibody-clone-32t77-m09168-boster.html2026-03-16T05:10:54+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aqp3-antibody-clone-32a78-m02181-boster.html2026-03-16T05:10:54+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-caspase-8-casp8-antibody-clone-32c79-m00042-6-boster.html2026-03-16T05:10:54+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cspp1-antibody-clone-32c80-m07521-boster.html2026-03-16T05:10:54+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vilip-1-vsnl1-antibody-clone-32v81-m06959-6-boster.html2026-03-16T05:10:54+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lypd3-antibody-clone-32l82-m08396-2-boster.html2026-03-16T05:10:54+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il13ra2-antibody-clone-32i83-m06105-1-boster.html2026-03-16T05:10:54+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-plp1-antibody-clone-32p84-m01056-boster.html2026-03-16T05:10:54+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pla2g4a-phospho-s505-antibody-clone-32p85-m00854-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rnf168-antibody-clone-32r86-m01224-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-asah1-antibody-clone-32a87-m02055-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tdp1-antibody-clone-32t88-m02231-1-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ilkap-antibody-clone-32i89-m08074-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pde10a-antibody-clone-32p90-m02605-2-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p21-cdkn1a-antibody-clone-32c91-m00145-8-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nmdar2b-grin2b-antibody-clone-32g92-m01883-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-npc2-antibody-clone-32n93-m01582-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-synpo-antibody-clone-32s94-m03154-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sestrin-2-sesn2-antibody-clone-32s95-m02558-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ythdf2-antibody-clone-32y96-m02621-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p16ink4a-cdkn2a-antibody-clone-32c97-m00016-6-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pi3k-p85-beta-pik3r2-antibody-clone-32p98-m03363-2-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nnos-nos1-antibody-clone-32n99-m01070-1-boster.html2026-03-16T05:10:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-isyna1-antibody-clone-33i00-m08693-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mitf-antibody-clone-33m01-m00269-5-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nlrp3-antibody-clone-33n02-m00034-2-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phf6-antibody-clone-33p03-m03065-1-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tim3-havcr2-antibody-clone-33h04-m00657-5-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fev-antibody-clone-33f05-m03460-1-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ppp2r1b-antibody-clone-33p06-m05756-1-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-f4-80-adgre1-antibody-clone-33a07-m08751-1-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd8b-antibody-clone-33c08-m07259-1-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dmt1-slc11a2-antibody-clone-33s09-m02622-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-osterix-sp7-antibody-clone-33s10-m02077-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mapk15-antibody-clone-33m11-m10088-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-laminin-gamma-2-lamc2-antibody-clone-33l12-m03214-boster.html2026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03214-lamc2-primary-antibodies-wb-testing-1.jpgAnti-Laminin gamma 2/LAMC2 Antibody (Monoclonal, 33L12)Western blot analysis of LAMC2/MRC1 using anti-LAMC2/MRC1 antibody (M03214). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMC2/MRC1 antigen affinity purified monoclonal antibody (M03214) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LAMC2/MRC1 at approximately 140 kDa. The expected band size for LAMC2/MRC1 is at 131 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03214-lamc2-primary-antibodies-ihc-testing-1.jpgAnti-Laminin gamma 2/LAMC2 Antibody (Monoclonal, 33L12)IHC analysis of LAMC2/MRC1 using anti-LAMC2/MRC1 antibody (M03214). LAMC2/MRC1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-LAMC2/MRC1 Antibody (M03214) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03214-lamc2-primary-antibodies-ihc-testing-2.jpgAnti-Laminin gamma 2/LAMC2 Antibody (Monoclonal, 33L12)IHC analysis of Laminin gamma 2/LAMC2 using anti-Laminin gamma 2/LAMC2 antibody (M03214). Laminin gamma 2/LAMC2 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Laminin gamma 2/LAMC2 Antibody (M03214) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03214-lamc2-primary-antibodies-ihc-testing-3.jpgAnti-Laminin gamma 2/LAMC2 Antibody (Monoclonal, 33L12)IHC analysis of Laminin gamma 2/LAMC2 using anti-Laminin gamma 2/LAMC2 antibody (M03214). Laminin gamma 2/LAMC2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Laminin gamma 2/LAMC2 Antibody (M03214) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kat6a-antibody-clone-33k13-m02949-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-irg1-acod1-antibody-clone-33a14-m13250-boster.html2026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m13250-acod1-primary-antibodies-wb-testing-1.jpgAnti-IRG1/ACOD1 Antibody (Monoclonal, 33A14)Western blot analysis of IRG1/ACOD1 using anti-IRG1/ACOD1 antibody (M13250). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human THP-1+LPS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRG1/ACOD1 antigen affinity purified monoclonal antibody (M13250) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IRG1/ACOD1 at approximately 54 kDa. The expected band size for IRG1/ACOD1 is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dlst-antibody-clone-33d15-m05097-boster.html2026-03-16T05:10:56+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-integrin-beta-5-itgb5-antibody-clone-33i16-m04201-1-boster.html2026-04-06T05:05:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mek6-map2k6-antibody-clone-33m17-m02011-3-boster.html2026-03-16T05:10:57+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dnajc6-antibody-clone-33d18-m05616-boster.html2026-03-16T05:10:57+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hmgcr-antibody-clone-33h19-m00643-1-boster.html2026-03-16T05:10:57+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-exosc9-antibody-clone-33e20-m07231-boster.html2026-03-16T05:10:57+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prkaca-antibody-clone-33p21-m00653-2-boster.html2026-03-16T05:10:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00653-2-prkaca-primary-antibodies-wb-testing-1.jpgAnti-PRKACA Antibody (Monoclonal, 33P21)Western blot analysis of PRKACA using anti-PRKACA antibody (M00653-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKACA antigen affinity purified monoclonal antibody (M00653-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRKACA at approximately 38 kDa. The expected band size for PRKACA is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00653-2-prkaca-primary-antibodies-ihc-testing-1.jpgAnti-PRKACA Antibody (Monoclonal, 33P21)IHC analysis of PRKACA using anti-PRKACA antibody (M00653-2). PRKACA was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-PRKACA Antibody (M00653-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nsd1-antibody-clone-33n22-m02327-boster.html2026-03-16T05:10:57+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-epha2-antibody-clone-33e23-m00578-3-boster.html2026-03-16T05:10:57+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sphk1-antibody-clone-33s24-m01390-2-boster.html2026-03-16T05:10:57+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ca12-antibody-clone-33c25-m04063-4-boster.html2026-03-16T05:10:57+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lactadherin-mfge8-antibody-clone-33m26-m02518-2-boster.html2026-03-16T05:10:57+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-spr-antibody-clone-33s27-m00416-3-boster.html2026-03-16T05:10:57+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pdgfrb-antibody-clone-33p28-m00096-7-boster.html2026-03-16T05:10:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00096-7-pdgfrb-primary-antibodies-wb-testing-1.jpgAnti-PDGFRB Antibody (Monoclonal, 33P28)Western blot analysis of PDGFRB using anti-PDGFRB antibody (M00096-7). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat L6 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse C2C12 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRB antigen affinity purified monoclonal antibody (M00096-7) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDGFRB at approximately 180 kDa. The expected band size for PDGFRB is at 123 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00096-7-pdgfrb-primary-antibodies-ihc-testing-1.jpgAnti-PDGFRB Antibody (Monoclonal, 33P28)IHC analysis of PDGFRB using anti-PDGFRB antibody (M00096-7). PDGFRB was detected in a paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-PDGFRB Antibody (M00096-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00096-7-pdgfrb-primary-antibodies-ihc-testing-2.jpgAnti-PDGFRB Antibody (Monoclonal, 33P28)IHC analysis of PDGFRB using anti-PDGFRB antibody (M00096-7). PDGFRB was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-PDGFRB Antibody (M00096-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00096-7-pdgfrb-primary-antibodies-ihc-testing-3.jpgAnti-PDGFRB Antibody (Monoclonal, 33P28)IHC analysis of PDGFRB using anti-PDGFRB antibody (M00096-7). PDGFRB was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-PDGFRB Antibody (M00096-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00096-7-pdgfrb-primary-antibodies-ihc-testing-4.jpgAnti-PDGFRB Antibody (Monoclonal, 33P28)IHC analysis of PDGFRB using anti-PDGFRB antibody (M00096-7). PDGFRB was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-PDGFRB Antibody (M00096-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-larp1-antibody-clone-33l29-m04488-1-boster.html2026-03-16T05:10:57+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd20-ms4a1-antibody-clone-33m30-m03780-20-boster.html2026-03-16T05:10:58+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atox1-antibody-clone-33a31-m03421-boster.html2026-03-16T05:10:58+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-giantin-golgb1-antibody-clone-33g32-m08226-1-boster.html2026-03-16T05:10:58+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-stra8-antibody-clone-33s33-m07955-boster.html2026-03-16T05:10:58+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cbx8-antibody-clone-33c34-m05234-3-boster.html2026-03-16T05:10:58+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-qprt-antibody-clone-33q35-m09896-1-boster.html2026-03-16T05:10:58+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd200-antibody-clone-33c36-m01512-2-boster.html2026-03-16T05:10:58+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hopx-antibody-clone-33h37-m05019-boster.html2026-03-16T05:10:58+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il-8-cxcl8-antibody-clone-33c38-m00423-5-boster.html2026-03-16T05:10:58+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bcl2-phospho-s70-antibody-clone-33b39-m00040-6-boster.html2026-03-16T05:10:58+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-baf170-smarcc2-antibody-clone-33s40-m06370-boster.html2026-03-16T05:10:58+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-psen2-antibody-clone-33p41-m00800-boster.html2026-03-16T05:10:58+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gamma-adaptin-ap1g1-antibody-clone-33a42-m12998-1-boster.html2026-03-16T05:10:59+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rhog-antibody-clone-33r43-m03485-boster.html2026-03-16T05:10:59+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-c15orf55-nutm1-antibody-clone-33n44-m10092-boster.html2026-03-16T05:10:59+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rab10-antibody-clone-33r45-m03588-1-boster.html2026-03-16T05:10:59+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-galc-antibody-clone-33g46-m01634-1-boster.html2026-03-16T05:10:59+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-adamts5-antibody-clone-33a47-m02802-boster.html2026-03-16T05:10:59+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hvem-tnfrsf14-antibody-clone-33t48-m02298-2-boster.html2026-03-16T05:10:59+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tau-mapt-phospho-t231-antibody-clone-33m49-m00097-12-boster.html2026-03-16T05:10:59+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mpg-antibody-clone-33m50-m00037-boster.html2026-03-16T05:10:59+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-upk3a-antibody-clone-33u51-m06534-2-boster.html2026-03-16T05:10:59+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-visa-mavs-antibody-clone-33m52-m00169-2-boster.html2026-03-16T05:10:59+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-perk-eif2ak3-antibody-clone-33e53-m01992-2-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-calca-antibody-clone-33c54-m02352-7-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-jmjd1a-kdm3a-antibody-clone-33k55-m03994-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-txnrd2-antibody-clone-33t56-m03900-2-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-syngap1-antibody-clone-33s57-m04862-1-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ve-cadherin-cdh5-antibody-clone-33c58-m02632-5-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ninein-nin-antibody-clone-33n59-m03001-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cpt1a-antibody-clone-33c60-m00917-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crebbp-antibody-clone-33c61-m00205-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sirt4-antibody-clone-33s62-m03764-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-creb1-antibody-clone-33c63-m00577-2-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bckdha-antibody-clone-33b64-m04561-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pericentrin-pcnt-antibody-clone-33p65-m03256-boster.html2026-03-16T05:11:00+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hsd11b1-antibody-clone-33h66-m01565-2-boster.html2026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01565-2-hsd11b1-primary-antibodies-wb-testing-1.jpgAnti-HSD11B1 Antibody (Monoclonal, 33H66)Western blot analysis of HSD11B1 using anti-HSD11B1 antibody (M01565-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B1 antigen affinity purified monoclonal antibody (M01565-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HSD11B1 at approximately 32 kDa. The expected band size for HSD11B1 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01565-2-hsd11b1-primary-antibodies-ihc-testing-1.jpgAnti-HSD11B1 Antibody (Monoclonal, 33H66)IHC analysis of HSD11B1 using anti-HSD11B1 antibody (M01565-2). HSD11B1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HSD11B1 Antibody (M01565-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01565-2-hsd11b1-primary-antibodies-ihc-testing-2.jpgAnti-HSD11B1 Antibody (Monoclonal, 33H66)IHC analysis of HSD11B1 using anti-HSD11B1 antibody (M01565-2). HSD11B1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HSD11B1 Antibody (M01565-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01565-2-hsd11b1-primary-antibodies-ihc-testing-3.jpgAnti-HSD11B1 Antibody (Monoclonal, 33H66)IHC analysis of HSD11B1 using anti-HSD11B1 antibody (M01565-2). HSD11B1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HSD11B1 Antibody (M01565-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trip4-antibody-clone-33t67-m07762-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rnf146-antibody-clone-33r68-m06525-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-human-iga-heavy-chain-igha1-antibody-clone-33i69-m07514-3-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tdg-antibody-clone-33t70-m01215-1-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-foxm1-antibody-clone-33f71-m00659-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tmc1-antibody-clone-33t72-m07763-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mip-1-alpha-ccl3-antibody-clone-33c73-m00405-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ube2k-antibody-clone-33u74-m05895-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-chd8-antibody-clone-33c75-m01346-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-importin-beta-kpnb1-antibody-clone-33k76-m01851-4-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eif4a2-antibody-clone-33e77-m06549-1-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cbx7-antibody-clone-33c78-m04742-1-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-axin2-antibody-clone-33a79-m01772-2-boster.html2026-03-16T05:11:01+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-abl1-antibody-clone-33a80-m00133-2-boster.html2026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00133-2-abl1-primary-antibodies-ihc-testing-1.jpgAnti-ABL1 Antibody (Monoclonal, 33A80)IHC analysis of ABL1 using anti-ABL1 antibody (M00133-2). ABL1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ABL1 Antibody (M00133-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00133-2-abl1-primary-antibodies-ihc-testing-2.jpgAnti-ABL1 Antibody (Monoclonal, 33A80)IHC analysis of ABL1 using anti-ABL1 antibody (M00133-2). ABL1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ABL1 Antibody (M00133-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-marco-antibody-clone-33m81-m00391-boster.html2026-03-16T05:11:02+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-grp78-bip-hspa5-antibody-clone-33h82-m00955-5-boster.html2026-03-16T05:11:02+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nsun5-antibody-clone-33n83-m11044-boster.html2026-03-16T05:11:02+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cftr-antibody-clone-33c84-m00028-1-boster.html2026-03-16T05:11:02+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cox5a-antibody-clone-33c85-m07895-boster.html2026-03-16T05:11:02+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-p504s-amacr-antibody-clone-33a86-m02217-4-boster.html2026-03-16T05:11:02+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sco2-antibody-clone-33s87-m02749-boster.html2026-03-16T05:11:02+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-amh-antibody-clone-33a88-m00763-boster.html2026-03-16T05:11:02+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-livin-birc7-antibody-clone-33b89-m02577-2-boster.html2026-03-16T05:11:02+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ulbp3-antibody-clone-33u90-m05191-boster.html2026-03-16T05:11:02+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-visa-mavs-antibody-clone-33m91-m00169-3-boster.html2026-03-16T05:11:02+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ganab-antibody-clone-33g92-m09668-boster.html2026-03-16T05:11:03+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pbx1-antibody-clone-33p93-m01039-1-boster.html2026-03-16T05:11:03+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dapk3-antibody-clone-33d94-m03300-boster.html2026-03-16T05:11:03+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-girk2-kcnj6-antibody-clone-33k95-m04558-boster.html2026-03-16T05:11:03+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sage1-antibody-clone-33s96-m15250-boster.html2026-03-16T05:11:03+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-laminin-gamma-1-lamc1-antibody-clone-33l97-m03522-2-boster.html2026-03-16T05:11:03+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-spon1-antibody-clone-33s98-m03094-boster.html2026-03-16T05:11:03+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-neurogranin-nrgn-antibody-clone-33n99-m05781-1-boster.html2026-03-16T05:11:03+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-b7h6-ncr3lg1-antibody-clone-34n00-m09747-1-boster.html2026-03-16T05:11:03+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd13-anpep-antibody-clone-34a01-m02591-7-boster.html2026-03-16T05:11:04+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-axl-antibody-clone-34a02-m00226-7-boster.html2026-03-16T05:11:04+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-drp1-dnm1l-phospho-s616-antibody-clone-34d03-m00556-4-boster.html2026-03-16T05:11:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00556-4-p-drp1-primary-antibodies-wb-testing-1.jpgAnti-DRP1/DNM1L (Phospho-S616) Antibody (Monoclonal, 34D03)Western blot analysis of P-DRP1 using anti-P-DRP1 antibody (M00556-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-DRP1 antigen affinity purified monoclonal antibody (M00556-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-DRP1 at approximately 75 kDa. The expected band size for P-DRP1 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00556-4-p-drp1-primary-antibodies-ihc-testing-1.jpgAnti-DRP1/DNM1L (Phospho-S616) Antibody (Monoclonal, 34D03)IHC analysis of P-DRP1 using anti-P-DRP1 antibody (M00556-4). P-DRP1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-P-DRP1 Antibody (M00556-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cops7b-antibody-clone-34c04-m12717-1-boster.html2026-03-16T05:11:04+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cyp11a1-antibody-clone-34c05-m01071-boster.html2026-03-16T05:11:04+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rlbp1-antibody-clone-34r06-m05421-3-boster.html2026-03-16T05:11:04+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tnks-antibody-clone-34t07-m02569-boster.html2026-03-16T05:11:04+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cingulin-cgn-antibody-clone-34c08-m02373-boster.html2026-03-16T05:11:04+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gabab-r1-gabbr1-antibody-clone-34g09-m07297-2-boster.html2026-03-16T05:11:04+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ythdc1-antibody-clone-34y10-m06172-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rorc-antibody-clone-34r11-m00444-1-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wee1-antibody-clone-34w12-m01319-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tgf-beta-3-tgfb3-antibody-clone-34t13-m01264-1-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cenpa-antibody-clone-34c14-m02989-1-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-aldehyde-oxidase-aox1-antibody-clone-34a15-m02144-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-irf8-antibody-clone-34i16-m00638-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trefoil-factor-1-tff1-antibody-clone-34t17-m01391-3-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sirt7-antibody-clone-34s18-m03022-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tomm40-antibody-clone-34t19-m03166-2-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rbmx2-antibody-clone-34r20-m13933-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gadd45b-antibody-clone-34g21-m03508-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mtf1-antibody-clone-34m22-m04733-1-boster.html2026-03-16T05:11:05+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cgas-antibody-clone-34c23-m31676-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-poldip2-antibody-clone-34p24-m09581-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lipt2-antibody-clone-34l25-m16619-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-crlf3-antibody-clone-34c26-m11434-1-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ncoa4-antibody-clone-34n27-m04368-2-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myf5-antibody-clone-34m28-m04040-2-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alpi-antibody-clone-34a29-m06115-2-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mapk12-antibody-clone-34m30-m03942-1-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rab1a-antibody-clone-34r31-m02682-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ezh2-antibody-clone-34e32-m00050-3-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-annexin-a1-anxa1-antibody-clone-34a33-m01451-4-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-prosurfactant-protein-c-sftpc-antibody-clone-34s34-m02001-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slp76-lcp2-antibody-clone-34l37-m04880-1-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-eef2k-antibody-clone-34e38-m02277-1-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-igf2bp1-antibody-clone-34i39-m02007-boster.html2026-03-16T05:11:06+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mt-co1-antibody-clone-34m40-m03773-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fabp3-antibody-clone-34f41-m01734-3-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-apaf1-antibody-clone-34a42-m00889-3-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fsp1-aifm2-antibody-clone-34a43-m06541-boster.html2026-03-16T05:11:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06541-aifm2-primary-antibodies-wb-testing-1.jpgAnti-FSP1/AIFM2 Antibody (Monoclonal, 34A43)Western blot analysis of FSP1/AIFM2 using anti-FSP1/AIFM2 antibody (M06541). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSP1/AIFM2 antigen affinity purified monoclonal antibody (M06541) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FSP1/AIFM2 at approximately 41 kDa. The expected band size for FSP1/AIFM2 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06541-aifm2-primary-antibodies-ihc-testing-1.jpgAnti-FSP1/AIFM2 Antibody (Monoclonal, 34A43)IHC analysis of FSP1/AIFM2/MRC1 using anti-FSP1/AIFM2/MRC1 antibody (M06541). FSP1/AIFM2/MRC1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-FSP1/AIFM2/MRC1 Antibody (M06541) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06541-fsp1-primary-antibodies-ihc-testing-1_1.jpgAnti-FSP1/AIFM2 Antibody (Monoclonal, 34A43)IHC analysis of FSP1 using anti-FSP1 antibody (M06541). FSP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-FSP1 Antibody (M06541) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alkbh5-antibody-clone-34a44-m03360-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-icos-antibody-clone-34i45-m00291-6-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-poll-antibody-clone-34p46-m00959-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-irf1-antibody-clone-34i47-m00580-1-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sil-stil-antibody-clone-34s48-m04956-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lkb1-stk11-antibody-clone-34s49-m00217-5-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pkm2-pkm-antibody-clone-34p50-m01173-3-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ccdc109a-mcu-antibody-clone-34m51-m00685-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ero1l-ero1a-antibody-clone-34e52-m06640-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rnf168-antibody-clone-34r53-m01224-1-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd11c-integrin-alpha-x-itgax-antibody-clone-34i54-m00357-3-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-brca2-antibody-clone-34b55-m00009-1-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mettl14-antibody-clone-34m56-m07389-1-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vti1b-antibody-clone-34v57-m07432-boster.html2026-03-16T05:11:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-vsig4-antibody-clone-34v58-m06371-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ndufa9-antibody-clone-34n59-m07420-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd56-ncam1-antibody-clone-34n60-m00184-7-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-zo-1-tjp1-antibody-clone-34t61-m00860-3-boster.html2026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00860-3-tjp1-primary-antibodies-wb-testing-1.jpgAnti-ZO-1/TJP1 Antibody (Monoclonal, 34T61)Western blot analysis of ZO-1/TJP1 using anti-ZO-1/TJP1 antibody (M00860-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZO-1/TJP1 antigen affinity purified monoclonal antibody (M00860-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ZO-1/TJP1 at approximately 240 kDa. The expected band size for ZO-1/TJP1 is at 195 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mip-2-cxcl2-antibody-clone-34c62-m01435-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hmgcs1-antibody-clone-34h63-m05313-boster.html2026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m05313-hmgcs1-primary-antibodies-wb-testing-1.jpgAnti-HMGCS1 Antibody (Monoclonal, 34H63)Western blot analysis of HMGCS1 using anti-HMGCS1 antibody (M05313). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGCS1 antigen affinity purified monoclonal antibody (M05313) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HMGCS1 at approximately 57 kDa. The expected band size for HMGCS1 is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-trim32-antibody-clone-34t64-m03750-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-igf1r-antibody-clone-34i65-m00070-13-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hla-a-antibody-clone-34h66-m00194-4-boster.html2026-03-16T05:11:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00194-4-hla-a-primary-antibodies-wb-testing-1.jpgAnti-HLA-A Antibody (Monoclonal, 34H66)Western blot analysis of HLA-A using anti-HLA-A antibody (M00194-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human HEL whole cell lysates, Lane 7: rat lung tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-A antigen affinity purified monoclonal antibody (M00194-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HLA-A at approximately 41,44 kDa. The expected band size for HLA-A is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lag3-antibody-clone-34l67-m02869-10-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd203c-enpp3-antibody-clone-34e68-m05615-2-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mig-cxcl9-antibody-clone-34c69-m01397-1-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bdh1-antibody-clone-34b70-m09277-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-hsp40-dnajb1-antibody-clone-34d71-m03100-1-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-dcc-antibody-clone-34d72-m00560-1-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-srpk1-antibody-clone-34s73-m01937-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-granzyme-b-gzmb-antibody-clone-34g74-m00353-4-boster.html2026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00353-4-gzmb-primary-antibodies-wb-testing-1.jpgAnti-Granzyme B/GZMB Antibody (Monoclonal, 34G74)Western blot analysis of Granzyme B/GZMB using anti-Granzyme B/GZMB antibody (M00353-4). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Granzyme B/GZMB antigen affinity purified monoclonal antibody (M00353-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Granzyme B/GZMB at approximately 30 kDa. The expected band size for Granzyme B/GZMB is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00353-4-gzmb-primary-antibodies-ihc-testing-1.jpgAnti-Granzyme B/GZMB Antibody (Monoclonal, 34G74)IHC analysis of Granzyme B/GZMB using anti-Granzyme B/GZMB antibody (M00353-4). Granzyme B/GZMB was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Granzyme B/GZMB Antibody (M00353-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-jmjd6-antibody-clone-34j75-m03690-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fxn-antibody-clone-34f76-m00842-1-boster.html2026-03-16T05:11:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myl9-phospho-t18-s19-antibody-clone-34m77-m06446-3-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rnf114-antibody-clone-34r78-m06969-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mcp-1-ccl2-antibody-clone-34c79-m00056-3-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smc5-antibody-clone-34s80-m01285-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-galc-antibody-clone-34g81-m01634-2-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-enos-nos3-phospho-s1177-antibody-clone-34n82-m01604-3-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-slc34a2-antibody-clone-34s83-m03957-3-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kdm5b-antibody-clone-34k84-m01620-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gitr-tnfrsf18-antibody-clone-34t85-m03125-6-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-xiap-antibody-clone-34x86-m00482-3-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lilrb1-antibody-clone-34l87-m03208-1-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-srebf1-antibody-clone-34s88-m00282-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd200r1-antibody-clone-34c89-m03707-1-boster.html2026-03-16T05:11:09+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nsun2-antibody-clone-34n90-m03878-boster.html2026-03-16T05:11:10+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-myh1-n-terminal-antibody-clone-34m91-m09420-boster.html2026-03-16T05:11:10+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd169-siglec1-antibody-clone-34s92-m05186-1-boster.html2026-03-16T05:11:10+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-med12-antibody-clone-34m93-m00828-1-boster.html2026-03-16T05:11:10+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ep300-antibody-clone-34e94-m00117-1-boster.html2026-03-16T05:11:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00117-1-ep300-primary-antibodies-ihc-testing-1.jpgAnti-EP300 Antibody (Monoclonal, 34E94)IHC analysis of EP300 using anti-EP300 antibody (M00117-1). EP300 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-EP300 Antibody (M00117-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00117-1-ep300-primary-antibodies-ihc-testing-2.jpgAnti-EP300 Antibody (Monoclonal, 34E94)IHC analysis of EP300 using anti-EP300 antibody (M00117-1). EP300 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-EP300 Antibody (M00117-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ryr2-antibody-clone-34r95-m00155-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00155-ryr2-primary-antibodies-ihc-testing-1.jpgAnti-RYR2 Antibody (Monoclonal, 34R95)IHC analysis of RYR2 using anti-RYR2 antibody (M00155). RYR2 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RYR2 Antibody (M00155) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00155-ryr2-primary-antibodies-ihc-testing-2.jpgAnti-RYR2 Antibody (Monoclonal, 34R95)IHC analysis of RYR2 using anti-RYR2 antibody (M00155). RYR2 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-RYR2 Antibody (M00155) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il-1-alpha-il1a-antibody-clone-34i96-m01144-5-boster.html2026-03-16T05:11:10+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-wwtr1-antibody-clone-34w97-m02672-2-boster.html2026-03-16T05:11:10+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-il-1-alpha-il1a-antibody-clone-34i98-m01144-6-boster.html2026-03-16T05:11:10+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-perforin-prf1-antibody-clone-34p99-m01429-2-boster.html2026-03-16T05:11:10+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-asgr1-antibody-clone-35a00-m05376-boster.html2026-03-16T05:11:10+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-phactr3-antibody-clone-35p01-m08700-boster.html2026-03-16T05:11:10+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-atp13a2-antibody-clone-35a02-m02601-boster.html2026-03-16T05:11:10+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rip3-ripk3-phospho-s232-antibody-clone-35r03-m00202-3-boster.html2026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00202-3-ripk3-primary-antibodies-wb-testing-1.jpgAnti-RIP3/RIPK3 (Phospho-S232) Antibody (Monoclonal, 35R03)Western blot analysis of RIP3/RIPK3 using anti-RIP3/RIPK3 antibody (M00202-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP3/RIPK3 antigen affinity purified monoclonal antibody (M00202-3) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RIP3/RIPK3 at approximately 57 kDa. The expected band size for RIP3/RIPK3 is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nsun6-antibody-clone-35n04-m13404-boster.html2026-03-16T05:11:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ncoa4-antibody-clone-35n05-m04368-3-boster.html2026-03-16T05:11:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sparc-antibody-clone-35s06-m00862-3-boster.html2026-03-16T05:11:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-collagen-type-ii-col2a1-antibody-clone-35c07-m00517-2-boster.html2026-03-16T05:11:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-notch4-antibody-clone-35n08-m02582-1-boster.html2026-03-16T05:11:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bnip3-antibody-clone-35b09-m01469-2-boster.html2026-03-16T05:11:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01469-2-bnip3-primary-antibodies-ihc-testing-1.jpgAnti-BNIP3 Antibody (Monoclonal, 35B09)IHC analysis of BNIP3 using anti-BNIP3 antibody (M01469-2). BNIP3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-BNIP3 Antibody (M01469-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01469-2-bnip3-primary-antibodies-ihc-testing-2.jpgAnti-BNIP3 Antibody (Monoclonal, 35B09)IHC analysis of BNIP3 using anti-BNIP3 antibody (M01469-2). BNIP3 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-BNIP3 Antibody (M01469-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01469-2-bnip3-primary-antibodies-ihc-testing-3.jpgAnti-BNIP3 Antibody (Monoclonal, 35B09)IHC analysis of BNIP3 using anti-BNIP3 antibody (M01469-2). BNIP3 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-BNIP3 Antibody (M01469-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-usp15-antibody-clone-35u10-m03057-boster.html2026-03-16T05:11:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-alpha-1-antitrypsin-serpina1a-antibody-clone-35s11-m33430-boster.html2026-03-16T05:11:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-kim-1-havcr1-antibody-clone-35h12-m01306-1-boster.html2026-03-16T05:11:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-camp-antibody-clone-35c13-m05475-boster.html2026-03-16T05:11:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mbd1-antibody-clone-35m14-m02336-2-boster.html2026-03-16T05:11:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sharp2-bhlhe40-antibody-clone-35b15-m02575-1-boster.html2026-03-16T05:11:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-nsun3-antibody-clone-35n16-m16002-boster.html2026-03-16T05:11:11+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ptgr1-antibody-clone-35p17-m08587-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tnfrsf5-cd40-antibody-clone-35c18-m00068-15-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-bre-babam2-antibody-clone-35b19-m32264-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-pgam5-antibody-clone-35p20-m05464-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cxcr3-antibody-clone-35c21-m00295-2-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-smc2-antibody-clone-35s22-m04804-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sdc4-antibody-clone-35s23-m02046-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gpnmb-antibody-clone-35g24-m02439-4-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-endothelin-1-edn1-antibody-clone-35e25-m01067-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-znrf2-antibody-clone-35z27-m11505-2-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-tph2-antibody-clone-35t28-m01643-1-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-acp1-antibody-clone-35a29-m02167-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-micb-antibody-clone-35m30-m01330-2-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-drd2-antibody-clone-35d31-m00289-1-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-noggin-nog-antibody-clone-35n32-m04448-1-boster.html2026-03-16T05:11:12+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-jchain-antibody-clone-35j33-m02644-1-boster.html2026-03-16T05:11:13+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-sumo3-antibody-clone-35s34-m05193-1-boster.html2026-03-16T05:11:13+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mfn1-antibody-clone-35m35-m02172-3-boster.html2026-03-16T05:11:13+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-rora-antibody-clone-35r36-m02266-1-boster.html2026-03-16T05:11:13+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-camkii-beta-camk2b-antibody-clone-35c37-m03964-5-boster.html2026-03-16T05:11:13+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fabp7-antibody-clone-35f38-m02646-boster.html2026-03-16T05:11:13+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-inos-nos2-antibody-clone-31n04-m00368-4-boster.html2026-03-16T05:11:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00368-4-fft270164-fig-0005-m.jpgAnti-iNOS/NOS2 Antibody (Monoclonal, 31N04)Effects of MT water extract on DSS-induced UC in zebrafishes. (A) Nontoxic effect of MT water extract (0.2–2 mg/mL) on the development of zebrafishes after 24, 48, and 72 hpf under photomicrograph. (B) Survival rate of zebrafishes. After 72 hpf, zebrafishes were treated with or without 0.5% DSS and co-treated with different concentrations of MT (0.2–2 mg/mL) for 3 days. (C and D) Effects of MT water extract (0.5–1 mg/mL) on the recruitment of neutrophils in DSS-induced IBD in zebrafish larvae. (C) Representative fluorescent images of different groups. Neutrophils recruited in the intestine were highlighted in the white area and zoomed for quantitative analysis (red rectangle). Scale bar: 250 µm. (D) Quantitative analysis of the number of neutrophils recruited in intestine. (E and F) Effects of MT water extract (0.5–1 mg/mL) on DSS-induced ROS production in WT zebrafish larvae. (F) Representative fluorescent images of ROS in different groups stained by CM-H2DCFDA. (F) Quantitative analysis of ROS levels in each group. (G) Effects of MT water extract (0.25–1 mg/mL) on DSS-induced inflammatory gene expressions (iNOS, COX-2, IL-6, and IL-1β) in zebrafish. Data were expressed as mean ± SD from at least three independent trials. One-way ANOVA followed by Tukey's multiple comparison test was performed to compare the differences between groups. *p < 0.05 versus control and #p < 0.05 versus DSS alone group. COX-2, cyclooxygenase-2; DSS, dextran sulfate sodium; ef1α, elongation factor 1α; IL, interleukin; iNOS, inducible nitric oxide synthase; ROS, reactive oxygen species. Index in Food Frontiers under a CC BY license. DOI: <a href='0'>10.1002/fft2.70164</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00368-4-fft270164-fig-0008-m.jpgAnti-iNOS/NOS2 Antibody (Monoclonal, 31N04)Effects of MT water extract on regulating macrophage polarization, maintaining intestinal barrier integrity, and alleviating oxidative stress in the colons of DSS-induced UC mice. (A) IHC staining of iNOS, Arg1, occludin, and ZO-1 expressions in mouse colon tissues. Quantitative analysis of the integrated optical density (IOD) of (B) iNOS, (C) Arg1, (D) occludin, and (E) ZO-1 in each group. The levels of (F) IL-6, (G) IL-10, (H) MDA, and (I) GSH were measured and analyzed. Data were presented as means ± SD for three independent trials. One-way ANOVA followed by Tukey's multiple comparison test was performed to compare the differences between groups. *p < 0.05 versus control and #p < 0.05 versus DSS alone group. Arg1, arginase 1; DSS, dextran sulfate sodium; IL, interleukin; iNOS, inducible nitric oxide synthase; MDA, malondialdehyde; MT, Medulla Tetrapanacis. Index in Food Frontiers under a CC BY license. DOI: <a href='0'>10.1002/fft2.70164</a> https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-cd206-mrc1-antibody-clone-31m03-m02285-3-boster.html2026-03-16T05:11:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02285-3-cd206-primary-antibodies-wb-testing-1.jpgAnti-CD206/MRC1 Antibody (Monoclonal, 31M03)Western blot analysis of CD206/MRC1 using anti-CD206/MRC1 antibody (M02285-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD206/MRC1 antigen affinity purified monoclonal antibody (M02285-3) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD206/MRC1 at approximately 180-200 kDa. The expected band size for CD206/MRC1 is at 165 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02285-3-cd206-primary-antibodies-ihc-testing-1.jpgAnti-CD206/MRC1 Antibody (Monoclonal, 31M03)IHC analysis of CD206/MRC1 using anti-CD206/MRC1 antibody (M02285-3). CD206/MRC1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CD206/MRC1 Antibody (M02285-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02285-3-cd206-primary-antibodies-ihc-testing-2.jpgAnti-CD206/MRC1 Antibody (Monoclonal, 31M03)IHC analysis of CD206/MRC1 using anti-CD206/MRC1 antibody (M02285-3). CD206/MRC1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CD206/MRC1 Antibody (M02285-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02285-3-cd206-primary-antibodies-ihc-testing-3.jpgAnti-CD206/MRC1 Antibody (Monoclonal, 31M03)IHC analysis of CD206/MRC1 using anti-CD206/MRC1 antibody (M02285-3). CD206/MRC1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CD206/MRC1 Antibody (M02285-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02285-3-cd206-primary-antibodies-ihc-testing-4.jpgAnti-CD206/MRC1 Antibody (Monoclonal, 31M03)IHC analysis of CD206/MRC1 using anti-CD206/MRC1 antibody (M02285-3). CD206/MRC1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CD206/MRC1 Antibody (M02285-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ampk-alpha-1-prkaa1-phospho-t183-ampk-alpha-2-prkaa2-phospho-t172-antibody-clone-31p05-m00994-3-boster.html2026-03-16T05:11:13+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-fasn-antibody-clone-31g06-m00941-3-boster.html2026-03-16T05:11:13+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-ghrelin-ghrl-antibody-clone-34g35-m01710-boster.html2026-03-16T05:11:13+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-mypt1-ppp1r12a-phospho-t696-antibody-clone-34p36-m01743-boster.html2026-03-16T05:11:13+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-myosin-skeletal-slow-antibody-monoclonal-m00697-boster.html2026-03-16T05:11:13+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329772026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329782026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329792026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-danio-rerio-zebrafish-timm23a-antibody-dz41727-boster.html2026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329812026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329822026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329832026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329842026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329852026-03-10T04:43:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41738-mamiko_yajima.pngAnti-Purple sea urchin LOC584590 AntibodyWestern blot analysis of LOC584590 using anti-LOC584590 antibody (DZ41738). Electrophoresis was performed on a 10% polyacrylamide gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Purple sea urchin embryo lysate lysates, After electrophoresis, proteins were transferred to a PVDF membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% BSA-TBST for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOC584590 antigen affinity purified polyclonal antibody (DZ41738) at 1:2000 with 1%BSA overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody for 2 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LOC584590 at approximately 60 kDa. https://www.bosterbio.com/catalog/product/view/id/1329862026-03-10T04:43:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/z/dz41739-mamiko_yajima.pngAnti-Purple sea urchin LOC581356 AntibodyWestern blot analysis of LOC581356 using anti-LOC581356 antibody (DZ41739). Electrophoresis was performed on a 10% polyacrylamide gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Purple sea urchin embryo lysate lysates, After electrophoresis, proteins were transferred to a PVDF membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% BSA-TBST for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOC581356 antigen affinity purified polyclonal antibody (DZ41739) at 1:2000 with 1%BSA overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody for 2 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LOC581356 at approximately 84 kDa. https://www.bosterbio.com/catalog/product/view/id/1329872026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329882026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329892026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329902026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329912026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329922026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329932026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329942026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329952026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329962026-03-10T04:43:07+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1329972026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00415-1-egln1-primary-antibodies-wb-testing-1.jpgAnti-PHD2/EGLN1 Antibody Picoband®Western blot analysis of PHD2/EGLN1 using anti-PHD2/EGLN1 antibody (A00415-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHD2/EGLN1 antigen affinity purified polyclonal antibody (A00415-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHD2/EGLN1 at approximately 46 kDa. The expected band size for PHD2/EGLN1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00415-1-egln1-primary-antibodies-ip-testing-1.jpgAnti-PHD2/EGLN1 Antibody Picoband®Immunoprecipitating PHD2/EGLN1 in A549 whole cell lysate. Western blot analysis of PHD2/EGLN1 using anti-PHD2/EGLN1 antibody (A00415-1). Lane 1: A549 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-PHD2/EGLN1 antibody in A549 whole cell lysate, Lane 3: anti-PHD2/EGLN1 antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PHD2/EGLN1 antigen affinity purified polyclonal antibody (A00415-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PHD2/EGLN1 at approximately 46 kDa. The expected band size for PHD2/EGLN1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00415-1-egln1-primary-antibodies-fcm-testing-1.jpgAnti-PHD2/EGLN1 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-PHD2/EGLN1 antibody (A00415-1). Overlay histogram showing SH-SY5Y cells stained with A00415-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHD2/EGLN1 Antibody (A00415-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-map1-antibody-monoclonal-m03897-boster.html2026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calbindin-d-antibody-monoclonal-m03047-3-boster.html2026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1330152026-03-10T04:43:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1330162026-03-10T04:43:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1330172026-03-10T04:43:08+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1330182026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08917-1-phc3-primary-antibodies-wb-testing-1.jpgAnti-PHC3 Antibody Picoband®Western blot analysis of PHC3 using anti-PHC3 antibody (A08917-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHC3 antigen affinity purified polyclonal antibody (A08917-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHC3 at approximately 106 kDa. The expected band size for PHC3 is at 106 kDa. https://www.bosterbio.com/catalog/product/view/id/1330192026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04822-1-phf2-primary-antibodies-wb-testing-1.jpgAnti-PHF2 Antibody Picoband®Western blot analysis of PHF2 using anti-PHF2 antibody (A04822-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human REH whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHF2 antigen affinity purified polyclonal antibody (A04822-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHF2 at approximately 121 kDa. The expected band size for PHF2 is at 121 kDa. https://www.bosterbio.com/catalog/product/view/id/1330202026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05558-3-orc6-primary-antibodies-wb-testing-1.jpgAnti-ORC6 Antibody Picoband®Western blot analysis of ORC6 using anti-ORC6 antibody (A05558-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORC6 antigen affinity purified polyclonal antibody (A05558-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ORC6 at approximately 28 kDa. The expected band size for ORC6 is at 28 kDa. https://www.bosterbio.com/catalog/product/view/id/1330212026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08702-1-calcoco1-primary-antibodies-wb-testing-1.jpgAnti-CALCOCO1 Antibody Picoband®Western blot analysis of CALCOCO1 using anti-CALCOCO1 antibody (A08702-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CALCOCO1 antigen affinity purified polyclonal antibody (A08702-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CALCOCO1 at approximately 77 kDa. The expected band size for CALCOCO1 is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08702-1-calcoco1-primary-antibodies-if-testing-1.jpgAnti-CALCOCO1 Antibody Picoband®IF analysis of CALCOCO1 using anti-CALCOCO1 antibody (A08702-1). CALCOCO1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CALCOCO1 Antibody (A08702-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1330222026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08465-1-pmf1-primary-antibodies-wb-testing-1.jpgAnti-PMF1 Antibody Picoband®Western blot analysis of PMF1 using anti-PMF1 antibody (A08465-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMF1 antigen affinity purified polyclonal antibody (A08465-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PMF1 at approximately 17 kDa. The expected band size for PMF1 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08465-1-pmf1-primary-antibodies-ihc-testing-1.jpgAnti-PMF1 Antibody Picoband®IHC analysis of PMF1 using anti-PMF1 antibody (A08465-1). PMF1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PMF1 Antibody (A08465-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08465-1-pmf1-primary-antibodies-fcm-testing-1.jpgAnti-PMF1 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-PMF1 antibody (A08465-1). Overlay histogram showing SH-SY5Y cells stained with A08465-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PMF1 Antibody (A08465-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330232026-03-17T05:17:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10431-1-pip4k2c-primary-antibodies-wb-testing-1.jpgAnti-PIP4K2C Antibody Picoband®Western blot analysis of PIP4K2C using anti-PIP4K2C antibody (A10431-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIP4K2C antigen affinity purified polyclonal antibody (A10431-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIP4K2C at approximately 47 kDa. The expected band size for PIP4K2C is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10431-1-pip4k2c-primary-antibodies-if-testing-1.jpgAnti-PIP4K2C Antibody Picoband®IF analysis of PIP4K2C using anti-PIP4K2C antibody (A10431-1) and anti-Alpha Tubulin antibody (M03989-3). PIP4K2C was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PIP4K2C Antibody (A10431-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1330242026-03-17T05:17:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06247-2-pkmyt1-primary-antibodies-wb-testing-1.jpgAnti-PKMYT1 Antibody Picoband®Western blot analysis of PKMYT1 using anti-PKMYT1 antibody (A06247-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human U2OS whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKMYT1 antigen affinity purified polyclonal antibody (A06247-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PKMYT1 at approximately 47 kDa. The expected band size for PKMYT1 is at 55 kDa. https://www.bosterbio.com/catalog/product/view/id/1330252026-03-17T05:17:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07166-1-pknox1-primary-antibodies-wb-testing-1.jpgAnti-PKNOX1 Antibody Picoband®Western blot analysis of PKNOX1 using anti-PKNOX1 antibody (A07166-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKNOX1 antigen affinity purified polyclonal antibody (A07166-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PKNOX1 at approximately 64 kDa. The expected band size for PKNOX1 is at 48 kDa. https://www.bosterbio.com/catalog/product/view/id/1330262026-03-17T05:17:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11848-3-pknox2-primary-antibodies-wb-testing-1.jpgAnti-PKNOX2 Antibody Picoband®Western blot analysis of PKNOX2 using anti-PKNOX2 antibody (A11848-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKNOX2 antigen affinity purified polyclonal antibody (A11848-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PKNOX2 at approximately 70 kDa. The expected band size for PKNOX2 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11848-3-pknox2-primary-antibodies-fcm-testing-1.jpgAnti-PKNOX2 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-PKNOX2 antibody (A11848-3). Overlay histogram showing SH-SY5Y cells stained with A11848-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKNOX2 Antibody (A11848-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330272026-03-17T05:17:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07633-pla2g10-primary-antibodies-wb-testing-1.jpgAnti-PLA2G10 Antibody Picoband®Western blot analysis of PLA2G10 using anti-PLA2G10 antibody (A07633). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G10 antigen affinity purified polyclonal antibody (A07633) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLA2G10 at approximately 18-20 kDa. The expected band size for PLA2G10 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07633-pla2g10-primary-antibodies-ihc-testing-1.jpgAnti-PLA2G10 Antibody Picoband®IHC analysis of PLA2G10 using anti-PLA2G10 antibody (A07633). PLA2G10 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLA2G10 Antibody (A07633) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07633-pla2g10-primary-antibodies-ihc-testing-2.jpgAnti-PLA2G10 Antibody Picoband®IHC analysis of PLA2G10 using anti-PLA2G10 antibody (A07633). PLA2G10 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLA2G10 Antibody (A07633) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07633-pla2g10-primary-antibodies-if-testing-1.jpgAnti-PLA2G10 Antibody Picoband®IF analysis of PLA2G10 using anti-PLA2G10 antibody (A07633). PLA2G10 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PLA2G10 Antibody (A07633) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07633-pla2g10-primary-antibodies-if-testing-2.jpgAnti-PLA2G10 Antibody Picoband®IF analysis of PLA2G10 using anti-PLA2G10 antibody (A07633). PLA2G10 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PLA2G10 Antibody (A07633) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07633-pla2g10-primary-antibodies-ip-testing-1.jpgAnti-PLA2G10 Antibody Picoband®Immunoprecipitating PLA2G10 in Caco-2 whole cell lysate. Western blot analysis of PLA2G10 using anti-PLA2G10 antibody (A07633). Lane 1: Caco-2 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-PLA2G10 antibody in Caco-2 whole cell lysate, Lane 3: anti-PLA2G10 antibody (2μg) + Caco-2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PLA2G10 antigen affinity purified polyclonal antibody (A07633) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PLA2G10 at approximately 20 kDa. The expected band size for PLA2G10 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07633-pla2g10-primary-antibodies-fcm-testing-1.jpgAnti-PLA2G10 Antibody Picoband®Flow Cytometry analysis of CACO-2 cells using anti-PLA2G10 antibody (A07633). Overlay histogram showing CACO-2 cells stained with A07633 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLA2G10 Antibody (A07633, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330282026-03-17T05:17:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10619-1-pla2g12a-primary-antibodies-wb-testing-1.jpgAnti-PLA2G12A Antibody Picoband®Western blot analysis of PLA2G12A using anti-PLA2G12A antibody (A10619-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G12A antigen affinity purified polyclonal antibody (A10619-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLA2G12A at approximately 24 kDa. The expected band size for PLA2G12A is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10619-1-pla2g12a-primary-antibodies-fcm-testing-1.jpgAnti-PLA2G12A Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-PLA2G12A antibody (A10619-1). Overlay histogram showing RT4 cells stained with A10619-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLA2G12A Antibody (A10619-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330292026-03-17T05:17:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02472-1-plag1-primary-antibodies-wb-testing-1.jpgAnti-PLAG1 Antibody Picoband®Western blot analysis of PLAG1 using anti-PLAG1 antibody (A02472-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAG1 antigen affinity purified polyclonal antibody (A02472-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLAG1 at approximately 60 kDa. The expected band size for PLAG1 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02472-1-plag1-primary-antibodies-fcm-testing-1.jpgAnti-PLAG1 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-PLAG1 antibody (A02472-1). Overlay histogram showing HEL cells stained with A02472-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLAG1 Antibody (A02472-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330302026-03-17T05:17:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08822-1-plb1-primary-antibodies-wb-testing-1.jpgAnti-PLB1 Antibody Picoband®Western blot analysis of PLB1 using anti-PLB1 antibody (A08822-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLB1 antigen affinity purified polyclonal antibody (A08822-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLB1 at approximately 163 kDa. The expected band size for PLB1 is at 163 kDa. https://www.bosterbio.com/catalog/product/view/id/1330312026-03-17T05:17:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07959-1-plekhm1-primary-antibodies-wb-testing-1.jpgAnti-PLEKHM1 Antibody Picoband®Western blot analysis of PLEKHM1 using anti-PLEKHM1 antibody (A07959-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLEKHM1 antigen affinity purified polyclonal antibody (A07959-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLEKHM1 at approximately 117 kDa. The expected band size for PLEKHM1 is at 117 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07959-1-plekhm1-primary-antibodies-if-testing-1.jpgAnti-PLEKHM1 Antibody Picoband®IF analysis of PLEKHM1 using anti-PLEKHM1 antibody (A07959-1). PLEKHM1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PLEKHM1 Antibody (A07959-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1330322026-03-17T05:17:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06734-1-ska1-primary-antibodies-wb-testing-1.jpgAnti-C18orf24/SKA1 Antibody Picoband®Western blot analysis of SKA1 using anti-SKA1 antibody (A06734-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse pancreas tissue lysates, Lane 5: mouse liver tissue lysates. Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKA1 antigen affinity purified polyclonal antibody (A06734-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SKA1 at approximately 32 kDa. The expected band size for SKA1 is at 29 kDa. https://www.bosterbio.com/catalog/product/view/id/1330332026-03-17T05:17:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08092-1-slc4a3-primary-antibodies-wb-testing-1.jpgAnti-SLC4A3 Antibody Picoband®Western blot analysis of SLC4A3 using anti-SLC4A3 antibody (A08092-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC4A3 antigen affinity purified polyclonal antibody (A08092-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC4A3 at approximately 150 kDa. The expected band size for SLC4A3 is at 136 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08092-1-slc4a3-primary-antibodies-wb-testing-2.jpgAnti-SLC4A3 Antibody Picoband®Western blot analysis of SLC4A3 using anti-SLC4A3 antibody (A08092-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC4A3 antigen affinity purified polyclonal antibody (A08092-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC4A3 at approximately 150 kDa. The expected band size for SLC4A3 is at 136 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08092-1-slc4a3-primary-antibodies-if-testing-1.jpgAnti-SLC4A3 Antibody Picoband®IF analysis of SLC4A3 using anti-SLC4A3 antibody (A08092-1) and anti-Alpha Tubulin antibody (M03989-3). SLC4A3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLC4A3 Antibody (A08092-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08092-1-slc4a3-primary-antibodies-fcm-testing-1.jpgAnti-SLC4A3 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-SLC4A3 antibody (A08092-1). Overlay histogram showing PC-3 cells stained with A08092-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC4A3 Antibody (A08092-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330342026-03-17T05:17:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10043-2-slc5a11-primary-antibodies-wb-testing-1.jpgAnti-SLC5A11 Antibody Picoband®Western blot analysis of SLC5A11 using anti-SLC5A11 antibody (A10043-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A11 antigen affinity purified polyclonal antibody (A10043-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC5A11 at approximately 70 kDa. The expected band size for SLC5A11 is at 74 kDa. https://www.bosterbio.com/catalog/product/view/id/1330352026-03-17T05:17:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14103-1-slc6a7-primary-antibodies-wb-testing-1.jpgAnti-SLC6A7 Antibody Picoband®Western blot analysis of SLC6A7 using anti-SLC6A7 antibody (A14103-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A7 antigen affinity purified polyclonal antibody (A14103-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC6A7 at approximately 71-75 kDa. The expected band size for SLC6A7 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14103-1-slc6a7-primary-antibodies-fcm-testing-1.jpgAnti-SLC6A7 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-SLC6A7 antibody (A14103-1). Overlay histogram showing U251 cells stained with A14103-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC6A7 Antibody (A14103-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330362026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07083-1-slc6a12-primary-antibodies-wb-testing-1.jpgAnti-SLC6A12 Antibody Picoband®Western blot analysis of SLC6A12 using anti-SLC6A12 antibody (A07083-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A12 antigen affinity purified polyclonal antibody (A07083-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC6A12 at approximately 76 kDa. The expected band size for SLC6A12 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07083-1-slc6a12-primary-antibodies-ihc-testing-1.jpgAnti-SLC6A12 Antibody Picoband®IHC analysis of SLC6A12 using anti-SLC6A12 antibody (A07083-1). SLC6A12 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC6A12 Antibody (A07083-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07083-1-slc6a12-primary-antibodies-ihc-testing-2.jpgAnti-SLC6A12 Antibody Picoband®IHC analysis of SLC6A12 using anti-SLC6A12 antibody (A07083-1). SLC6A12 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC6A12 Antibody (A07083-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07083-1-slc6a12-primary-antibodies-ihc-testing-3.jpgAnti-SLC6A12 Antibody Picoband®IHC analysis of SLC6A12 using anti-SLC6A12 antibody (A07083-1). SLC6A12 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC6A12 Antibody (A07083-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1330372026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05010-1-plaa-primary-antibodies-wb-testing-1.jpgAnti-PLAA Antibody Picoband®Western blot analysis of PLAA using anti-PLAA antibody (A05010-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAA antigen affinity purified polyclonal antibody (A05010-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PLAA at approximately 70 kDa. The expected band size for PLAA is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05010-1-plaa-primary-antibodies-fcm-testing-1.jpgAnti-PLAA Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-PLAA antibody (A05010-1). Overlay histogram showing SH-SY5Y cells stained with A05010-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLAA Antibody (A05010-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330382026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14207-1-trub1-primary-antibodies-wb-testing-1.jpgAnti-TRUB1 Antibody Picoband®Western blot analysis of TRUB1 using anti-TRUB1 antibody (A14207-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRUB1 antigen affinity purified polyclonal antibody (A14207-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRUB1 at approximately 37-39 kDa. The expected band size for TRUB1 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14207-1-trub1-primary-antibodies-ihc-testing-1.jpgAnti-TRUB1 Antibody Picoband®IHC analysis of TRUB1 using anti-TRUB1 antibody (A14207-1). TRUB1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRUB1 Antibody (A14207-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14207-1-trub1-primary-antibodies-if-testing-1.jpgAnti-TRUB1 Antibody Picoband®IF analysis of TRUB1 using anti-TRUB1 antibody (A14207-1). TRUB1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TRUB1 Antibody (A14207-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14207-1-trub1-primary-antibodies-ip-testing-1.jpgAnti-TRUB1 Antibody Picoband®Immunoprecipitating TRUB1 in HepG2 whole cell lysate. Western blot analysis of TRUB1 using anti-TRUB1 antibody (A14207-1). Lane 1: HepG2 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TRUB1 antibody in HepG2 whole cell lysate, Lane 3: anti-TRUB1 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRUB1 antigen affinity purified polyclonal antibody (A14207-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TRUB1 at approximately 37-39 kDa. The expected band size for TRUB1 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14207-1-trub1-primary-antibodies-fcm-testing-1.jpgAnti-TRUB1 Antibody Picoband®Flow Cytometry analysis of A431 cells using anti-TRUB1 antibody (A14207-1). Overlay histogram showing A431 cells stained with A14207-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRUB1 Antibody (A14207-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330392026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02589-smg1-primary-antibodies-wb-testing-1.jpgAnti-SMG1 Antibody Picoband®Western blot analysis of SMG1 using anti-SMG1 antibody (A02589). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Saos-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMG1 antigen affinity purified polyclonal antibody (A02589) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMG1 at approximately 400 kDa. The expected band size for SMG1 is at 411 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02589-smg1-primary-antibodies-if-testing-1.jpgAnti-SMG1 Antibody Picoband®IF analysis of SMG1 using anti-SMG1 antibody (A02589). SMG1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SMG1 Antibody (A02589) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1330402026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04238-2-grk4-primary-antibodies-wb-testing-1.jpgAnti-GRK4 Antibody Picoband®Western blot analysis of GRK4 using anti-GRK4 antibody (A04238-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRK4 antigen affinity purified polyclonal antibody (A04238-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRK4 at approximately 67 kDa. The expected band size for GRK4 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04238-2-grk4-primary-antibodies-if-testing-1.jpgAnti-GRK4 Antibody Picoband®IF analysis of GRK4 using anti-GRK4 antibody (A04238-2). GRK4 was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GRK4 Antibody (A04238-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1330412026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03032-1-slc22a4-primary-antibodies-wb-testing-1.jpgAnti-SLC22A4 Antibody Picoband®Western blot analysis of SLC22A4 using anti-SLC22A4 antibody (A03032-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC22A4 antigen affinity purified polyclonal antibody (A03032-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC22A4 at approximately 62 kDa. The expected band size for SLC22A4 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03032-1-slc22a4-primary-antibodies-fcm-testing-1.jpgAnti-SLC22A4 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-SLC22A4 antibody (A03032-1). Overlay histogram showing MCF-7 cells stained with A03032-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC22A4 Antibody (A03032-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330422026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04445-slc23a1-primary-antibodies-wb-testing-1.jpgAnti-SLC23A1 Antibody Picoband®Western blot analysis of SLC23A1 using anti-SLC23A1 antibody (A04445). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC23A1 antigen affinity purified polyclonal antibody (A04445) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC23A1 at approximately 65 kDa. The expected band size for SLC23A1 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04445-slc23a1-primary-antibodies-fcm-testing-1.jpgAnti-SLC23A1 Antibody Picoband®Flow Cytometry analysis of CACO-2 cells using anti-SLC23A1 antibody (A04445). Overlay histogram showing CACO-2 cells stained with A04445 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC23A1 Antibody (A04445, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330432026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16236-slc23a3-primary-antibodies-wb-testing-1.jpgAnti-SLC23A3 Antibody Picoband®Western blot analysis of SLC23A3 using anti-SLC23A3 antibody (A16236). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC23A3 antigen affinity purified polyclonal antibody (A16236) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC23A3 at approximately 65 kDa. The expected band size for SLC23A3 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16236-slc23a3-primary-antibodies-if-testing-1.jpgAnti-SLC23A3 Antibody Picoband®IF analysis of SLC23A3 using anti-SLC23A3 antibody (A16236). SLC23A3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLC23A3 Antibody (A16236) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16236-slc23a3-primary-antibodies-fcm-testing-1.jpgAnti-SLC23A3 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-SLC23A3 antibody (A16236). Overlay histogram showing RT4 cells stained with A16236 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC23A3 Antibody (A16236, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330442026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11621-1-slc24a2-primary-antibodies-wb-testing-1.jpgAnti-SLC24A2 Antibody Picoband®Western blot analysis of SLC24A2 using anti-SLC24A2 antibody (A11621-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC24A2 antigen affinity purified polyclonal antibody (A11621-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC24A2 at approximately 74 kDa. The expected band size for SLC24A2 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11621-1-slc24a2-primary-antibodies-fcm-testing-1.jpgAnti-SLC24A2 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-SLC24A2 antibody (A11621-1). Overlay histogram showing U251 cells stained with A11621-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC24A2 Antibody (A11621-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330452026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10976-2-slc25a17-primary-antibodies-wb-testing-1.jpgAnti-SLC25A17 Antibody Picoband®Western blot analysis of SLC25A17 using anti-SLC25A17 antibody (A10976-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A17 antigen affinity purified polyclonal antibody (A10976-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A17 at approximately 46 kDa. The expected band size for SLC25A17 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10976-2-slc25a17-primary-antibodies-if-testing-1.jpgAnti-SLC25A17 Antibody Picoband®IF analysis of SLC25A17 using anti-SLC25A17 antibody (A10976-2). SLC25A17 was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLC25A17 Antibody (A10976-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1330462026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11791-1-slc25a21-primary-antibodies-wb-testing-1.jpgAnti-SLC25A21 Antibody Picoband®Western blot analysis of SLC25A21 using anti-SLC25A21 antibody (A11791-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A21 antigen affinity purified polyclonal antibody (A11791-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A21 at approximately 36 kDa. The expected band size for SLC25A21 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11791-1-slc25a21-primary-antibodies-ihc-testing-1.jpgAnti-SLC25A21 Antibody Picoband®IHC analysis of SLC25A21 using anti-SLC25A21 antibody (A11791-1). SLC25A21 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A21 Antibody (A11791-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11791-1-slc25a21-primary-antibodies-if-testing-1.jpgAnti-SLC25A21 Antibody Picoband®IF analysis of SLC25A21 using anti-SLC25A21 antibody (A11791-1). SLC25A21 was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLC25A21 Antibody (A11791-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1330472026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09782-2-slc25a27-primary-antibodies-wb-testing-1.jpgAnti-SLC25A27 Antibody Picoband®Western blot analysis of SLC25A27 using anti-SLC25A27 antibody (A09782-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SK-N-SH whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A27 antigen affinity purified polyclonal antibody (A09782-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A27 at approximately 36 kDa. The expected band size for SLC25A27 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09782-2-slc25a27-primary-antibodies-ihc-testing-1.jpgAnti-SLC25A27 Antibody Picoband®IHC analysis of SLC25A27 using anti-SLC25A27 antibody (A09782-2). SLC25A27 was detected in a paraffin-embedded section of mouse spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A27 Antibody (A09782-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09782-2-slc25a27-primary-antibodies-ihc-testing-2.jpgAnti-SLC25A27 Antibody Picoband®IHC analysis of SLC25A27 using anti-SLC25A27 antibody (A09782-2). SLC25A27 was detected in a paraffin-embedded section of rat spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A27 Antibody (A09782-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1330482026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14204-1-slc25a39-primary-antibodies-wb-testing-1.jpgAnti-SLC25A39 Antibody Picoband®Western blot analysis of SLC25A39 using anti-SLC25A39 antibody (A14204-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A39 antigen affinity purified polyclonal antibody (A14204-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A39 at approximately 39 kDa. The expected band size for SLC25A39 is at 39 kDa. https://www.bosterbio.com/catalog/product/view/id/1330492026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15684-1-slc25a44-primary-antibodies-wb-testing-1.jpgAnti-SLC25A44 Antibody Picoband®Western blot analysis of SLC25A44 using anti-SLC25A44 antibody (A15684-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A44 antigen affinity purified polyclonal antibody (A15684-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A44 at approximately 35 kDa. The expected band size for SLC25A44 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15684-1-slc25a44-primary-antibodies-ihc-testing-1.jpgAnti-SLC25A44 Antibody Picoband®IHC analysis of SLC25A44 using anti-SLC25A44 antibody (A15684-1). SLC25A44 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A44 Antibody (A15684-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1330502026-03-17T05:17:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14907-1-slc25a45-primary-antibodies-wb-testing-1.jpgAnti-SLC25A45 Antibody Picoband®Western blot analysis of SLC25A45 using anti-SLC25A45 antibody (A14907-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human REH whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A45 antigen affinity purified polyclonal antibody (A14907-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A45 at approximately 32 kDa. The expected band size for SLC25A45 is at 27 kDa. https://www.bosterbio.com/catalog/product/view/id/1330512026-03-17T05:17:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07470-1-slc26a1-primary-antibodies-wb-testing-1.jpgAnti-SLC26A1 Antibody Picoband®Western blot analysis of SLC26A1 using anti-SLC26A1 antibody (A07470-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC26A1 antigen affinity purified polyclonal antibody (A07470-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC26A1 at approximately 75 kDa. The expected band size for SLC26A1 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07470-1-slc26a1-primary-antibodies-ihc-testing-1.jpgAnti-SLC26A1 Antibody Picoband®IHC analysis of SLC26A1 using anti-SLC26A1 antibody (A07470-1). SLC26A1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC26A1 Antibody (A07470-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1330522026-03-17T05:17:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08288-1-slc26a8-primary-antibodies-wb-testing-1.jpgAnti-SLC26A8 Antibody Picoband®Western blot analysis of SLC26A8 using anti-SLC26A8 antibody (A08288-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: rat RG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC26A8 antigen affinity purified polyclonal antibody (A08288-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC26A8 at approximately 70 kDa. The expected band size for SLC26A8 is at 109 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08288-1-slc26a8-primary-antibodies-if-testing-1.jpgAnti-SLC26A8 Antibody Picoband®IF analysis of SLC26A8 using anti-SLC26A8 antibody (A08288-1). SLC26A8 was detected in an immunocytochemical section of Raw264.7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLC26A8 Antibody (A08288-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08288-1-slc26a8-primary-antibodies-fcm-testing-1.jpgAnti-SLC26A8 Antibody Picoband®Flow Cytometry analysis of RAW264.7 cells using anti-SLC26A8 antibody (A08288-1). Overlay histogram showing RAW264.7 cells stained with A08288-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC26A8 Antibody (A08288-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330532026-03-17T05:17:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05925-2-slc26a5-primary-antibodies-wb-testing-1.jpgAnti-SLC26A5 Antibody Picoband®Western blot analysis of SLC26A5 using anti-SLC26A5 antibody (A05925-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC26A5 antigen affinity purified polyclonal antibody (A05925-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC26A5 at approximately 95 kDa. The expected band size for SLC26A5 is at 81 kDa. https://www.bosterbio.com/catalog/product/view/id/1330542026-03-17T05:17:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14683-1-slc26a10-primary-antibodies-wb-testing-1.jpgAnti-SLC26A10P Antibody Picoband®Western blot analysis of SLC26A10 using anti-SLC26A10 antibody (A14683-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HGC-27 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC26A10 antigen affinity purified polyclonal antibody (A14683-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC26A10 at approximately 60 kDa. The expected band size for SLC26A10 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14683-1-slc26a10-primary-antibodies-ihc-testing-1.jpgAnti-SLC26A10P Antibody Picoband®IHC analysis of SLC26A10 using anti-SLC26A10 antibody (A14683-1). SLC26A10 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC26A10 Antibody (A14683-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1330552026-03-17T05:17:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12006-slc51b-primary-antibodies-ihc-testing-1.jpgAnti-OSTbeta/SLC51B AntibodyIHC analysis of SLC51B using anti-SLC51B antibody (A12006). SLC51B was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC51B Antibody (A12006) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12006-slc51b-primary-antibodies-fcm-testing-1.jpgAnti-OSTbeta/SLC51B AntibodyFlow Cytometry analysis of HEPA1-6 cells using anti-SLC51B antibody (A12006). Overlay histogram showing HEPA1-6 cells stained with A12006 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC51B Antibody (A12006, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330562026-03-17T05:17:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18877-1-slco1b7-primary-antibodies-wb-testing-1.jpgAnti-SLCO1B7 Antibody Picoband®Western blot analysis of SLCO1B7 using anti-SLCO1B7 antibody (A18877-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLCO1B7 antigen affinity purified polyclonal antibody (A18877-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLCO1B7 at approximately 100 kDa. The expected band size for SLCO1B7 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18877-1-slco1b7-primary-antibodies-if-testing-1.jpgAnti-SLCO1B7 Antibody Picoband®IF analysis of SLCO1B7 using anti-SLCO1B7 antibody (A18877-1). SLCO1B7 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLCO1B7 Antibody (A18877-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a18877-1-slco1b7-primary-antibodies-fcm-testing-1.jpgAnti-SLCO1B7 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-SLCO1B7 antibody (A18877-1). Overlay histogram showing A549 cells stained with A18877-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLCO1B7 Antibody (A18877-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330572026-03-17T05:17:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14357-2-prickle3-primary-antibodies-wb-testing-1.jpgAnti-PRICKLE3 Antibody Picoband®Western blot analysis of PRICKLE3 using anti-PRICKLE3 antibody (A14357-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse stomach tissue lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRICKLE3 antigen affinity purified polyclonal antibody (A14357-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRICKLE3 at approximately 69 kDa. The expected band size for PRICKLE3 is at 69 kDa. https://www.bosterbio.com/catalog/product/view/id/1330582026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05904-prrc2a-primary-antibodies-wb-testing-1.jpgAnti-PRRC2A Antibody Picoband®Western blot analysis of PRRC2A using anti-PRRC2A antibody (A05904). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: mouse ovary tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRRC2A antigen affinity purified polyclonal antibody (A05904) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRRC2A at approximately 230 kDa. The expected band size for PRRC2A is at 229 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05904-prrc2a-primary-antibodies-if-testing-1.jpgAnti-PRRC2A Antibody Picoband®IF analysis of PRRC2A using anti-PRRC2A antibody (A05904). PRRC2A was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRRC2A Antibody (A05904) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1330592026-03-17T05:17:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12638-3-slc7a4-primary-antibodies-wb-testing-1.jpgAnti-SLC7A4 Antibody Picoband®Western blot analysis of SLC7A4 using anti-SLC7A4 antibody (A12638-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC7A4 antigen affinity purified polyclonal antibody (A12638-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC7A4 at approximately 75 kDa. The expected band size for SLC7A4 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12638-3-slc7a4-primary-antibodies-fcm-testing-1.jpgAnti-SLC7A4 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-SLC7A4 antibody (A12638-3). Overlay histogram showing HEL cells stained with A12638-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC7A4 Antibody (A12638-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330602026-03-17T05:17:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09335-2-slc7a10-primary-antibodies-wb-testing-1.jpgAnti-SLC7A10 Antibody Picoband®Western blot analysis of SLC7A10 using anti-SLC7A10 antibody (A09335-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC7A10 antigen affinity purified polyclonal antibody (A09335-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC7A10 at approximately 57 kDa. The expected band size for SLC7A10 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09335-2-slc7a10-primary-antibodies-fcm-testing-1.jpgAnti-SLC7A10 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-SLC7A10 antibody (A09335-2). Overlay histogram showing HepG2 cells stained with A09335-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC7A10 Antibody (A09335-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330612026-03-17T05:17:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08337-1-slc5a3-primary-antibodies-wb-testing-1.jpgAnti-SLC5A3 Antibody Picoband®Western blot analysis of SLC5A3 using anti-SLC5A3 antibody (A08337-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A3 antigen affinity purified polyclonal antibody (A08337-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC5A3 at approximately 58 kDa. The expected band size for SLC5A3 is at 58 kDa. https://www.bosterbio.com/catalog/product/view/id/1330622026-03-17T05:17:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12080-1-daam2-primary-antibodies-wb-testing-1.jpgAnti-DAAM2 Antibody Picoband®Western blot analysis of DAAM2 using anti-DAAM2 antibody (A12080-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAAM2 antigen affinity purified polyclonal antibody (A12080-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DAAM2 at approximately 100 kDa. The expected band size for DAAM2 is at 123 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12080-1-daam2-primary-antibodies-ihc-testing-1.jpgAnti-DAAM2 Antibody Picoband®IHC analysis of DAAM2 using anti-DAAM2 antibody (A12080-1). DAAM2 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAAM2 Antibody (A12080-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12080-1-daam2-primary-antibodies-ihc-testing-2.jpgAnti-DAAM2 Antibody Picoband®IHC analysis of DAAM2 using anti-DAAM2 antibody (A12080-1). DAAM2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAAM2 Antibody (A12080-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12080-1-daam2-primary-antibodies-if-testing-1.jpgAnti-DAAM2 Antibody Picoband®IF analysis of DAAM2 using anti-DAAM2 antibody (A12080-1) and anti-Alpha Tubulin antibody (M03989-3). DAAM2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DAAM2 Antibody (A12080-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12080-1-daam2-primary-antibodies-fcm-testing-1.jpgAnti-DAAM2 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-DAAM2 antibody (A12080-1). Overlay histogram showing Jurkat cells stained with A12080-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAAM2 Antibody (A12080-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330632026-03-17T05:17:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17793-2-dalrd3-primary-antibodies-wb-testing-1.jpgAnti-DALRD3 Antibody Picoband®Western blot analysis of DALRD3 using anti-DALRD3 antibody (A17793-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DALRD3 antigen affinity purified polyclonal antibody (A17793-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DALRD3 at approximately 59 kDa. The expected band size for DALRD3 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17793-2-dalrd3-primary-antibodies-fcm-testing-1.jpgAnti-DALRD3 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-DALRD3 antibody (A17793-2). Overlay histogram showing K562 cells stained with A17793-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DALRD3 Antibody (A17793-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330642026-03-17T05:17:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11812-2-dand5-primary-antibodies-ihc-testing-1.jpgAnti-DAND5 AntibodyIHC analysis of DAND5 using anti-DAND5 antibody (A11812-2). DAND5 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAND5 Antibody (A11812-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11812-2-dand5-primary-antibodies-ihc-testing-2.jpgAnti-DAND5 AntibodyIHC analysis of DAND5 using anti-DAND5 antibody (A11812-2). DAND5 was detected in a paraffin-embedded section of mouse eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAND5 Antibody (A11812-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11812-2-dand5-primary-antibodies-ihc-testing-3.jpgAnti-DAND5 AntibodyIHC analysis of DAND5 using anti-DAND5 antibody (A11812-2). DAND5 was detected in a paraffin-embedded section of rat eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAND5 Antibody (A11812-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1330652026-03-17T05:17:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05131-3-dap3-primary-antibodies-wb-testing-1.jpgAnti-DAP3 Antibody Picoband®Western blot analysis of DAP3 using anti-DAP3 antibody (A05131-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAP3 antigen affinity purified polyclonal antibody (A05131-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DAP3 at approximately 40 kDa. The expected band size for DAP3 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05131-3-dap3-primary-antibodies-ihc-testing-1.jpgAnti-DAP3 Antibody Picoband®IHC analysis of DAP3 using anti-DAP3 antibody (A05131-3). DAP3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAP3 Antibody (A05131-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1330662026-03-17T05:17:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10861-1-slc25a32-primary-antibodies-wb-testing-1.jpgAnti-SLC25A32 Antibody Picoband®Western blot analysis of SLC25A32 using anti-SLC25A32 antibody (A10861-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A32 antigen affinity purified polyclonal antibody (A10861-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A32 at approximately 50 kDa. The expected band size for SLC25A32 is at 35 kDa. https://www.bosterbio.com/catalog/product/view/id/1330672026-03-17T05:17:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11323-2-slc25a33-primary-antibodies-wb-testing-1.jpgAnti-SLC25A33 Antibody Picoband®Western blot analysis of SLC25A33 using anti-SLC25A33 antibody (A11323-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A33 antigen affinity purified polyclonal antibody (A11323-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A33 at approximately 35 kDa. The expected band size for SLC25A33 is at 35 kDa. https://www.bosterbio.com/catalog/product/view/id/1330682026-03-17T05:17:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15961-1-snx24-primary-antibodies-wb-testing-1.jpgAnti-SNX24 Antibody Picoband®Western blot analysis of SNX24 using anti-SNX24 antibody (A15961-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX24 antigen affinity purified polyclonal antibody (A15961-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNX24 at approximately 24 kDa. The expected band size for SNX24 is at 20 kDa. https://www.bosterbio.com/catalog/product/view/id/1330692026-03-17T05:17:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17611-1-snx32-primary-antibodies-wb-testing-1.jpgAnti-SNX32 Antibody Picoband®Western blot analysis of SNX32 using anti-SNX32 antibody (A17611-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX32 antigen affinity purified polyclonal antibody (A17611-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNX32 at approximately 46 kDa. The expected band size for SNX32 is at 46 kDa. https://www.bosterbio.com/catalog/product/view/id/1330702026-03-17T05:17:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02051-1-sp100-primary-antibodies-wb-testing-1.jpgAnti-SP100 Antibody Picoband®Western blot analysis of SP100 using anti-SP100 antibody (A02051-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP100 antigen affinity purified polyclonal antibody (A02051-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SP100 at approximately 80-90 kDa. The expected band size for SP100 is at 55 kDa. https://www.bosterbio.com/catalog/product/view/id/1330712026-03-17T05:17:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09286-1-sp140-primary-antibodies-wb-testing-1.jpgAnti-SP140 Antibody Picoband®Western blot analysis of SP140 using anti-SP140 antibody (A09286-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse small intestine tissue lysates, Lane 5: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP140 antigen affinity purified polyclonal antibody (A09286-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SP140 at approximately 100 kDa. The expected band size for SP140 is at 98 kDa. https://www.bosterbio.com/catalog/product/view/id/1330722026-03-17T05:17:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03882-3-prdm9-primary-antibodies-wb-testing-1.jpgAnti-PRDM9 Antibody Picoband®Western blot analysis of PRDM9 using anti-PRDM9 antibody (A03882-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM9 antigen affinity purified polyclonal antibody (A03882-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRDM9 at approximately 103 kDa. The expected band size for PRDM9 is at 103 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03882-3-prdm9-primary-antibodies-ihc-testing-1.jpgAnti-PRDM9 Antibody Picoband®IHC analysis of PRDM9 using anti-PRDM9 antibody (A03882-3). PRDM9 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRDM9 Antibody (A03882-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03882-3-prdm9-primary-antibodies-ihc-testing-2.jpgAnti-PRDM9 Antibody Picoband®IHC analysis of PRDM9 using anti-PRDM9 antibody (A03882-3). PRDM9 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRDM9 Antibody (A03882-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03882-3-prdm9-primary-antibodies-fcm-testing-1.jpgAnti-PRDM9 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-PRDM9 antibody (A03882-3). Overlay histogram showing PC-3 cells stained with A03882-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM9 Antibody (A03882-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330732026-03-17T05:17:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05605-2-pnoc-primary-antibodies-wb-testing-1.jpgAnti-PNOC Antibody Picoband®Western blot analysis of PNOC using anti-PNOC antibody (A05605-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PNOC antigen affinity purified polyclonal antibody (A05605-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PNOC at approximately 20 kDa. The expected band size for PNOC is at 20 kDa. https://www.bosterbio.com/catalog/product/view/id/1330742026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06287-1-lmx1a-primary-antibodies-wb-testing-1.jpgAnti-LMX1A Antibody Picoband®Western blot analysis of LMX1A using anti-LMX1A antibody (A06287-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMX1A antigen affinity purified polyclonal antibody (A06287-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LMX1A at approximately 43 kDa. The expected band size for LMX1A is at 43 kDa. https://www.bosterbio.com/catalog/product/view/id/1330752026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09248-1-ddx25-primary-antibodies-wb-testing-1.jpgAnti-DDX25 Antibody Picoband®Western blot analysis of DDX25 using anti-DDX25 antibody (A09248-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human OVCAR-8 whole cell lysates, Lane 3: rat ovary tissue lysates, Lane 4: rat testos tissue lysates, Lane 5: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX25 antigen affinity purified polyclonal antibody (A09248-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX25 at approximately 55 kDa. The expected band size for DDX25 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09248-1-ddx25-primary-antibodies-ihc-testing-1.jpgAnti-DDX25 Antibody Picoband®IHC analysis of DDX25 using anti-DDX25 antibody (A09248-1). DDX25 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX25 Antibody (A09248-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09248-1-ddx25-primary-antibodies-fcm-testing-1.jpgAnti-DDX25 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-DDX25 antibody (A09248-1). Overlay histogram showing SH-SY5Y cells stained with A09248-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX25 Antibody (A09248-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330762026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05407-2-ddx41-primary-antibodies-wb-testing-1.jpgAnti-DDX41 Antibody Picoband®Western blot analysis of DDX41 using anti-DDX41 antibody (A05407-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX41 antigen affinity purified polyclonal antibody (A05407-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX41 at approximately 70 kDa. The expected band size for DDX41 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05407-2-ddx41-primary-antibodies-ihc-testing-1.jpgAnti-DDX41 Antibody Picoband®IHC analysis of DDX41 using anti-DDX41 antibody (A05407-2). DDX41 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX41 Antibody (A05407-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05407-2-ddx41-primary-antibodies-ihc-testing-2.jpgAnti-DDX41 Antibody Picoband®IHC analysis of DDX41 using anti-DDX41 antibody (A05407-2). DDX41 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX41 Antibody (A05407-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1330772026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11206-3-ddx51-primary-antibodies-wb-testing-1.jpgAnti-DDX51 Antibody Picoband®Western blot analysis of DDX51 using anti-DDX51 antibody (A11206-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX51 antigen affinity purified polyclonal antibody (A11206-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX51 at approximately 72 kDa. The expected band size for DDX51 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11206-3-ddx51-primary-antibodies-fcm-testing-1.jpgAnti-DDX51 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-DDX51 antibody (A11206-3). Overlay histogram showing PC-3 cells stained with A11206-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX51 Antibody (A11206-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330782026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08923-2-ddx54-primary-antibodies-wb-testing-1.jpgAnti-DDX54 Antibody Picoband®Western blot analysis of DDX54 using anti-DDX54 antibody (A08923-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX54 antigen affinity purified polyclonal antibody (A08923-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX54 at approximately 99 kDa. The expected band size for DDX54 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08923-2-ddx54-primary-antibodies-fcm-testing-1.jpgAnti-DDX54 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-DDX54 antibody (A08923-2). Overlay histogram showing PC-3 cells stained with A08923-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX54 Antibody (A08923-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330792026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15035-2-slc22a14-primary-antibodies-ihc-testing-1.jpgAnti-SLC22A14 AntibodyIHC analysis of SLC22A14 using anti-SLC22A14 antibody (A15035-2). SLC22A14 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC22A14 Antibody (A15035-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15035-2-slc22a14-primary-antibodies-ihc-testing-2.jpgAnti-SLC22A14 AntibodyIHC analysis of SLC22A14 using anti-SLC22A14 antibody (A15035-2). SLC22A14 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC22A14 Antibody (A15035-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15035-2-slc22a14-primary-antibodies-fcm-testing-1.jpgAnti-SLC22A14 AntibodyFlow Cytometry analysis of CACO-2 cells using anti-SLC22A14 antibody (A15035-2). Overlay histogram showing CACO-2 cells stained with A15035-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC22A14 Antibody (A15035-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330802026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05863-1-st3gal4-primary-antibodies-wb-testing-1.jpgAnti-ST3GAL4 Antibody Picoband®Western blot analysis of ST3GAL4 using anti-ST3GAL4 antibody (A05863-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST3GAL4 antigen affinity purified polyclonal antibody (A05863-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ST3GAL4 at approximately 50 kDa. The expected band size for ST3GAL4 is at 38 kDa. https://www.bosterbio.com/catalog/product/view/id/1330812026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13389-1-spink9-primary-antibodies-ihc-testing-1.jpgAnti-SPINK9 AntibodyIHC analysis of SPINK9 using anti-SPINK9 antibody (A13389-1). SPINK9 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPINK9 Antibody (A13389-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13389-1-spink9-primary-antibodies-ihc-testing-2.jpgAnti-SPINK9 AntibodyIHC analysis of SPINK9 using anti-SPINK9 antibody (A13389-1). SPINK9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SPINK9 Antibody (A13389-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1330822026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10987-stard9-primary-antibodies-wb-testing-1.jpgAnti-STARD9 Antibody Picoband®Western blot analysis of STARD9 using anti-STARD9 antibody (A10987). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STARD9 antigen affinity purified polyclonal antibody (A10987) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STARD9 at approximately 54 kDa. The expected band size for STARD9 is at 516 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10987-stard9-primary-antibodies-fcm-testing-1.jpgAnti-STARD9 Antibody Picoband®Flow Cytometry analysis of U87 cells using anti-STARD9 antibody (A10987). Overlay histogram showing U87 cells stained with A10987 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STARD9 Antibody (A10987, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330832026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11379-1-sugp1-primary-antibodies-wb-testing-1.jpgAnti-SUGP1 Antibody Picoband®Western blot analysis of SUGP1 using anti-SUGP1 antibody (A11379-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUGP1 antigen affinity purified polyclonal antibody (A11379-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SUGP1 at approximately 72 kDa. The expected band size for SUGP1 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11379-1-sugp1-primary-antibodies-if-testing-1.jpgAnti-SUGP1 Antibody Picoband®IF analysis of SUGP1 using anti-SUGP1 antibody (A11379-1) and anti-Alpha Tubulin antibody (M03989-3). SUGP1 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SUGP1 Antibody (A11379-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11379-1-sugp1-primary-antibodies-fcm-testing-1.jpgAnti-SUGP1 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-SUGP1 antibody (A11379-1). Overlay histogram showing U251 cells stained with A11379-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUGP1 Antibody (A11379-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330842026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08807-1-snrpg-primary-antibodies-wb-testing-1.jpgAnti-SNRPG Antibody Picoband®Western blot analysis of SNRPG using anti-SNRPG antibody (A08807-1). Electrophoresis was performed on a 15% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRPG antigen affinity purified polyclonal antibody (A08807-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNRPG at approximately 9 kDa. The expected band size for SNRPG is at 8 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08807-1-snrpg-primary-antibodies-if-testing-1.jpgAnti-SNRPG Antibody Picoband®IF analysis of SNRPG using anti-SNRPG antibody (A08807-1) and anti-Alpha Tubulin antibody (M03989-3). SNRPG was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNRPG Antibody (A08807-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1330852026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06078-2-smg7-primary-antibodies-wb-testing-1.jpgAnti-SMG7 Antibody Picoband®Western blot analysis of SMG7 using anti-SMG7 antibody (A06078-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMG7 antigen affinity purified polyclonal antibody (A06078-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMG7 at approximately 132 kDa. The expected band size for SMG7 is at 127 kDa. https://www.bosterbio.com/catalog/product/view/id/1330862026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05386-1-slc36a1-primary-antibodies-wb-testing-1.jpgAnti-SLC36A1 Antibody Picoband®Western blot analysis of SLC36A1 using anti-SLC36A1 antibody (A05386-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC36A1 antigen affinity purified polyclonal antibody (A05386-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC36A1 at approximately 53 kDa. The expected band size for SLC36A1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05386-1-slc36a1-primary-antibodies-fcm-testing-1.jpgAnti-SLC36A1 Antibody Picoband®Flow Cytometry analysis of U87 cells using anti-SLC36A1 antibody (A05386-1). Overlay histogram showing U87 cells stained with A05386-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC36A1 Antibody (A05386-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330872026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10400-1-gjd3-primary-antibodies-ihc-testing-1.jpgAnti-GJD3 AntibodyIHC analysis of GJD3 using anti-GJD3 antibody (A10400-1). GJD3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJD3 Antibody (A10400-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10400-1-gjd3-primary-antibodies-ihc-testing-2.jpgAnti-GJD3 AntibodyIHC analysis of GJD3 using anti-GJD3 antibody (A10400-1). GJD3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJD3 Antibody (A10400-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1330882026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07114-2-gpr3-primary-antibodies-wb-testing-1.jpgAnti-GPR3 Antibody Picoband®Western blot analysis of GPR3 using anti-GPR3 antibody (A07114-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPR3 antigen affinity purified polyclonal antibody (A07114-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GPR3 at approximately 45 kDa. The expected band size for GPR3 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07114-2-gpr3-primary-antibodies-ihc-testing-1.jpgAnti-GPR3 Antibody Picoband®IHC analysis of GPR3 using anti-GPR3 antibody (A07114-2). GPR3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPR3 Antibody (A07114-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07114-2-gpr3-primary-antibodies-if-testing-1.jpgAnti-GPR3 Antibody Picoband®IF analysis of GPR3 using anti-GPR3 antibody (A07114-2). GPR3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GPR3 Antibody (A07114-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1330892026-03-17T05:17:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08570-1-ndst2-primary-antibodies-wb-testing-1.jpgAnti-NDST2 Antibody Picoband®Western blot analysis of NDST2 using anti-NDST2 antibody (A08570-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDST2 antigen affinity purified polyclonal antibody (A08570-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NDST2 at approximately 130-140 kDa. The expected band size for NDST2 is at 101 kDa. https://www.bosterbio.com/catalog/product/view/id/1330902026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03850-3-bcl2a1-primary-antibodies-wb-testing-1.jpgAnti-BCL2A1 Antibody Picoband®Western blot analysis of BCL2A1 using anti-BCL2A1 antibody (A03850-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2A1 antigen affinity purified polyclonal antibody (A03850-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BCL2A1 at approximately 27 kDa. The expected band size for BCL2A1 is at 27 kDa. https://www.bosterbio.com/catalog/product/view/id/1330912026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16438-tmem208-primary-antibodies-wb-testing-1.jpgAnti-TMEM208 Antibody Picoband®Western blot analysis of TMEM208 using anti-TMEM208 antibody (A16438). Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM208 antigen affinity purified polyclonal antibody (A16438) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMEM208 at approximately 19 kDa. The expected band size for TMEM208 is at 19 kDa. https://www.bosterbio.com/catalog/product/view/id/1330922026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12376-1-lrrn3-primary-antibodies-wb-testing-1.jpgAnti-LRRN3 Antibody Picoband®Western blot analysis of LRRN3 using anti-LRRN3 antibody (A12376-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SK-N-SH whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRN3 antigen affinity purified polyclonal antibody (A12376-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LRRN3 at approximately 79 kDa. The expected band size for LRRN3 is at 79 kDa. https://www.bosterbio.com/catalog/product/view/id/1330932026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07328-2-ly86-primary-antibodies-wb-testing-1.jpgAnti-LY86 Antibody Picoband®Western blot analysis of LY86 using anti-LY86 antibody (A07328-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LY86 antigen affinity purified polyclonal antibody (A07328-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LY86 at approximately 27 kDa. The expected band size for LY86 is at 18 kDa. https://www.bosterbio.com/catalog/product/view/id/1330942026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00363-2-tnfsf11-primary-antibodies-wb-testing-1.jpgAnti-TNFSF11 Antibody Picoband®Western blot analysis of TNFSF11 using anti-TNFSF11 antibody (A00363-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse thymus tissue lysates, Lane 5: mouse colon tissue lysates, Lane 6: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFSF11 antigen affinity purified polyclonal antibody (A00363-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNFSF11 at approximately 35 kDa. The expected band size for TNFSF11 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00363-2-tnfsf11-primary-antibodies-ihc-testing-1.jpgAnti-TNFSF11 Antibody Picoband®IHC analysis of TNFSF11 using anti-TNFSF11 antibody (A00363-2). TNFSF11 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNFSF11 Antibody (A00363-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00363-2-tnfsf11-primary-antibodies-ihc-testing-2.jpgAnti-TNFSF11 Antibody Picoband®IHC analysis of TNFSF11 using anti-TNFSF11 antibody (A00363-2). TNFSF11 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNFSF11 Antibody (A00363-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00363-2-tnfsf11-primary-antibodies-ihc-testing-3.jpgAnti-TNFSF11 Antibody Picoband®IHC analysis of TNFSF11 using anti-TNFSF11 antibody (A00363-2). TNFSF11 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNFSF11 Antibody (A00363-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1330952026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01555-1-phip-primary-antibodies-wb-testing-1.jpgAnti-PHIP Antibody Picoband®Western blot analysis of PHIP using anti-PHIP antibody (A01555-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHIP antigen affinity purified polyclonal antibody (A01555-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PHIP at approximately 207 kDa. The expected band size for PHIP is at 206 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01555-1-phip-primary-antibodies-if-testing-1.jpgAnti-PHIP Antibody Picoband®IF analysis of PHIP using anti-PHIP antibody (A01555-1). PHIP was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PHIP Antibody (A01555-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01555-1-phip-primary-antibodies-fcm-testing-1.jpgAnti-PHIP Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-PHIP antibody (A01555-1). Overlay histogram showing A549 cells stained with A01555-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PHIP Antibody (A01555-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330962026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07097-1-sfi1-primary-antibodies-wb-testing-1.jpgAnti-SFI1 Antibody Picoband®Western blot analysis of SFI1 using anti-SFI1 antibody (A07097-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFI1 antigen affinity purified polyclonal antibody (A07097-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SFI1 at approximately 160 kDa. The expected band size for SFI1 is at 147 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07097-1-sfi1-primary-antibodies-fcm-testing-1.jpgAnti-SFI1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-SFI1 antibody (A07097-1). Overlay histogram showing HepG2 cells stained with A07097-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFI1 Antibody (A07097-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330972026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07847-1-serpinb7-primary-antibodies-wb-testing-1.jpgAnti-SERPINB7 Antibody Picoband®Western blot analysis of SERPINB7 using anti-SERPINB7 antibody (A07847-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse MFC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINB7 antigen affinity purified polyclonal antibody (A07847-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SERPINB7 at approximately 43 kDa. The expected band size for SERPINB7 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07847-1-serpinb7-primary-antibodies-ihc-testing-1.jpgAnti-SERPINB7 Antibody Picoband®IHC analysis of SERPINB7 using anti-SERPINB7 antibody (A07847-1). SERPINB7 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERPINB7 Antibody (A07847-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07847-1-serpinb7-primary-antibodies-ihc-testing-2.jpgAnti-SERPINB7 Antibody Picoband®IHC analysis of SERPINB7 using anti-SERPINB7 antibody (A07847-1). SERPINB7 was detected in a paraffin-embedded section of rat skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERPINB7 Antibody (A07847-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07847-1-serpinb7-primary-antibodies-ihc-testing-3.jpgAnti-SERPINB7 Antibody Picoband®IHC analysis of SERPINB7 using anti-SERPINB7 antibody (A07847-1). SERPINB7 was detected in a paraffin-embedded section of mouse skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERPINB7 Antibody (A07847-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1330982026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07406-1-ece2-primary-antibodies-wb-testing-1.jpgAnti-ECE2 Antibody Picoband®Western blot analysis of ECE2 using anti-ECE2 antibody (A07406-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECE2 antigen affinity purified polyclonal antibody (A07406-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ECE2 at approximately 91 kDa. The expected band size for ECE2 is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07406-1-ece2-primary-antibodies-fcm-testing-1.jpgAnti-ECE2 Antibody Picoband®Flow Cytometry analysis of CACO-2 cells using anti-ECE2 antibody (A07406-1). Overlay histogram showing CACO-2 cells stained with A07406-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ECE2 Antibody (A07406-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1330992026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07803-2-atg10-primary-antibodies-wb-testing-1.jpgAnti-ATG10 Antibody Picoband®Western blot analysis of ATG10 using anti-ATG10 antibody (A07803-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG10 antigen affinity purified polyclonal antibody (A07803-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATG10 at approximately 25 kDa. The expected band size for ATG10 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07803-2-atg10-primary-antibodies-ihc-testing-1.jpgAnti-ATG10 Antibody Picoband®IHC analysis of ATG10 using anti-ATG10 antibody (A07803-2). ATG10 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATG10 Antibody (A07803-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07803-2-atg10-primary-antibodies-ihc-testing-2.jpgAnti-ATG10 Antibody Picoband®IHC analysis of ATG10 using anti-ATG10 antibody (A07803-2). ATG10 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATG10 Antibody (A07803-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07803-2-atg10-primary-antibodies-if-testing-1.jpgAnti-ATG10 Antibody Picoband®IF analysis of ATG10 using anti-ATG10 antibody (A07803-2). ATG10 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ATG10 Antibody (A07803-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07803-2-atg10-primary-antibodies-if-testing-2.jpgAnti-ATG10 Antibody Picoband®IF analysis of ATG10 using anti-ATG10 antibody (A07803-2). ATG10 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATG10 Antibody (A07803-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07803-2-atg10-primary-antibodies-fcm-testing-1.jpgAnti-ATG10 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-ATG10 antibody (A07803-2). Overlay histogram showing HepG2 cells stained with A07803-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG10 Antibody (A07803-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1331002026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04292-2-fzd5-primary-antibodies-wb-testing-1.jpgAnti-FZD5 AntibodyWestern blot analysis of FZD5 using anti-FZD5 antibody (A04292-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FZD5 antigen affinity purified polyclonal antibody (A04292-2) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FZD5 at approximately 70 kDa. The expected band size for FZD5 is at 65 kDa. https://www.bosterbio.com/catalog/product/view/id/1331012026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331022026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331032026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331042026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331052026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331062026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331072026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331082026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331092026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331102026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331112026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331122026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331132026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331142026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331152026-03-16T05:11:14+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331162026-03-16T05:11:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331172026-03-16T05:11:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331182026-03-16T05:11:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331192026-03-16T05:11:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331202026-03-16T05:11:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331212026-03-16T05:11:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331222026-03-16T05:11:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331232026-03-16T05:11:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331242026-03-16T05:11:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331252026-03-16T05:11:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331262026-03-16T05:11:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331272026-03-16T05:11:15+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331282026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331292026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331302026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331312026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331322026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331332026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331342026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331352026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331362026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331372026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331382026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13366-1-dhrs1-primary-antibodies-wb-testing-1.jpgAnti-DHRS1 Antibody Picoband®Western blot analysis of DHRS1 using anti-DHRS1 antibody (A13366-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHRS1 antigen affinity purified polyclonal antibody (A13366-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHRS1 at approximately 35 kDa. The expected band size for DHRS1 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13366-1-dhrs1-primary-antibodies-ihc-testing-1.jpgAnti-DHRS1 Antibody Picoband®IHC analysis of DHRS1 using anti-DHRS1 antibody (A13366-1). DHRS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHRS1 Antibody (A13366-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13366-1-dhrs1-primary-antibodies-if-testing-1.jpgAnti-DHRS1 Antibody Picoband®IF analysis of DHRS1 using anti-DHRS1 antibody (A13366-1) and anti-Alpha Tubulin antibody (M03989-3). DHRS1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DHRS1 Antibody (A13366-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13366-1-dhrs1-primary-antibodies-fcm-testing-1.jpgAnti-DHRS1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-DHRS1 antibody (A13366-1). Overlay histogram showing HepG2 cells stained with A13366-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DHRS1 Antibody (A13366-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1331392026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12691-1-dhrs7-primary-antibodies-wb-testing-1.jpgAnti-DHRS7 Antibody Picoband®Western blot analysis of DHRS7 using anti-DHRS7 antibody (A12691-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHRS7 antigen affinity purified polyclonal antibody (A12691-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHRS7 at approximately 36,38 kDa. The expected band size for DHRS7 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12691-1-dhrs7-primary-antibodies-ihc-testing-1.jpgAnti-DHRS7 Antibody Picoband®IHC analysis of DHRS7 using anti-DHRS7 antibody (A12691-1). DHRS7 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHRS7 Antibody (A12691-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1331402026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331412026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331422026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331432026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331442026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331452026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331462026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331472026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09445-tmed2-primary-antibodies-wb-testing-1.jpgAnti-TMED2 Antibody Picoband®Western blot analysis of TMED2 using anti-TMED2 antibody (A09445). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMED2 antigen affinity purified polyclonal antibody (A09445) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMED2 at approximately 20 kDa. The expected band size for TMED2 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09445-tmed2-primary-antibodies-ihc-testing-1.jpgAnti-TMED2 Antibody Picoband®IHC analysis of TMED2 using anti-TMED2 antibody (A09445). TMED2 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMED2 Antibody (A09445) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1331482026-03-16T05:11:16+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331492026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331502026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331512026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331522026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331532026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331542026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331552026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331562026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331572026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16001-dnajc4-primary-antibodies-wb-testing-1.jpgAnti-DNAJC4 Antibody Picoband®Western blot analysis of DNAJC4 using anti-DNAJC4 antibody (A16001). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJC4 antigen affinity purified polyclonal antibody (A16001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNAJC4 at approximately 28 kDa. The expected band size for DNAJC4 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16001-dnajc4-primary-antibodies-ihc-testing-1.jpgAnti-DNAJC4 Antibody Picoband®IHC analysis of DNAJC4 using anti-DNAJC4 antibody (A16001). DNAJC4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAJC4 Antibody (A16001) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1331582026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331592026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331602026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331612026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331622026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331632026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03128-2-cyp2j2-primary-antibodies-wb-testing-1.jpgAnti-CYP2J2 Antibody Picoband®Western blot analysis of CYP2J2 using anti-CYP2J2 antibody (A03128-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human Jurkat whole cell lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2J2 antigen affinity purified polyclonal antibody (A03128-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CYP2J2 at approximately 58 kDa. The expected band size for CYP2J2 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03128-2-cyp2j2-primary-antibodies-fcm-testing-1.jpgAnti-CYP2J2 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-CYP2J2 antibody (A03128-2). Overlay histogram showing Jurkat cells stained with A03128-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP2J2 Antibody (A03128-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1331642026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331652026-03-16T05:11:17+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331662026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09919-1-dusp7-primary-antibodies-wb-testing-1.jpgAnti-PYST2/DUSP7 Antibody Picoband®Western blot analysis of PYST2/DUSP7 using anti-PYST2/DUSP7 antibody (A09919-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYST2/DUSP7 antigen affinity purified polyclonal antibody (A09919-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PYST2/DUSP7 at approximately 50 kDa. The expected band size for PYST2/DUSP7 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09919-1-dusp7-primary-antibodies-ihc-testing-1.jpgAnti-PYST2/DUSP7 Antibody Picoband®IHC analysis of PYST2/DUSP7 using anti-PYST2/DUSP7 antibody (A09919-1). PYST2/DUSP7 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYST2/DUSP7 Antibody (A09919-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09919-1-dusp7-primary-antibodies-ihc-testing-2.jpgAnti-PYST2/DUSP7 Antibody Picoband®IHC analysis of PYST2/DUSP7 using anti-PYST2/DUSP7 antibody (A09919-1). PYST2/DUSP7 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PYST2/DUSP7 Antibody (A09919-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1331672026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15137-dnajb13-primary-antibodies-wb-testing-1.jpgAnti-DNAJB13 Antibody Picoband®Western blot analysis of DNAJB13 using anti-DNAJB13 antibody (A15137). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJB13 antigen affinity purified polyclonal antibody (A15137) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNAJB13 at approximately 36 kDa. The expected band size for DNAJB13 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15137-dnajb13-primary-antibodies-wb-testing-2.jpgAnti-DNAJB13 Antibody Picoband®Western blot analysis of DNAJB13 using anti-DNAJB13 antibody (A15137). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJB13 antigen affinity purified polyclonal antibody (A15137) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNAJB13 at approximately 36 kDa. The expected band size for DNAJB13 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15137-dnajb13-primary-antibodies-ihc-testing-1.jpgAnti-DNAJB13 Antibody Picoband®IHC analysis of DNAJB13 using anti-DNAJB13 antibody (A15137). DNAJB13 was detected in a paraffin-embedded section of human fallopian tube tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAJB13 Antibody (A15137) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15137-dnajb13-primary-antibodies-ihc-testing-2.jpgAnti-DNAJB13 Antibody Picoband®IHC analysis of DNAJB13 using anti-DNAJB13 antibody (A15137). DNAJB13 was detected in a paraffin-embedded section of human fallopian tube tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAJB13 Antibody (A15137) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15137-dnajb13-primary-antibodies-fcm-testing-1.jpgAnti-DNAJB13 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-DNAJB13 antibody (A15137). Overlay histogram showing Jurkat cells stained with A15137 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAJB13 Antibody (A15137, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1331682026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331692026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331702026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331712026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331722026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331732026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331742026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331752026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331762026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331772026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331782026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331792026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331802026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331812026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331822026-03-16T05:11:18+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331832026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331842026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331852026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331862026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331872026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331882026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331892026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331902026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331912026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331922026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09964-1-rp9-primary-antibodies-wb-testing-1.jpgAnti-RP9 Antibody Picoband®Western blot analysis of RP9 using anti-RP9 antibody (A09964-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RP9 antigen affinity purified polyclonal antibody (A09964-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RP9 at approximately 34 kDa. The expected band size for RP9 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09964-1-rp9-primary-antibodies-ihc-testing-1.jpgAnti-RP9 Antibody Picoband®IHC analysis of RP9 using anti-RP9 antibody (A09964-1). RP9 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RP9 Antibody (A09964-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09964-1-rp9-primary-antibodies-ihc-testing-2.jpgAnti-RP9 Antibody Picoband®IHC analysis of RP9 using anti-RP9 antibody (A09964-1). RP9 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RP9 Antibody (A09964-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09964-1-rp9-primary-antibodies-fcm-testing-1.jpgAnti-RP9 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-RP9 antibody (A09964-1). Overlay histogram showing SH-SY5Y cells stained with A09964-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-RP9 Antibody (A09964-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1331932026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331942026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331952026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331962026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331972026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331982026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1331992026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332002026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332012026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332022026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332032026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332042026-03-16T05:11:19+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332052026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332062026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332072026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332082026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332092026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332102026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332112026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332122026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332132026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332142026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332152026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332162026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332172026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332182026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332192026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332202026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332212026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332222026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332232026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332242026-03-16T05:11:20+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332252026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332262026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05875-1-dpp8-primary-antibodies-wb-testing-1.jpgAnti-DPP8 Antibody Picoband®Western blot analysis of DPP8 using anti-DPP8 antibody (A05875-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPP8 antigen affinity purified polyclonal antibody (A05875-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DPP8 at approximately 103 kDa. The expected band size for DPP8 is at 103 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05875-1-dpp8-primary-antibodies-fcm-testing-1.jpgAnti-DPP8 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-DPP8 antibody (A05875-1). Overlay histogram showing RT4 cells stained with A05875-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DPP8 Antibody (A05875-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1332272026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08536-drap1-primary-antibodies-wb-testing-1.jpgAnti-DRAP1 Antibody Picoband®Western blot analysis of DRAP1 using anti-DRAP1 antibody (A08536). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRAP1 antigen affinity purified polyclonal antibody (A08536) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DRAP1 at approximately 26 kDa. The expected band size for DRAP1 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08536-drap1-primary-antibodies-ihc-testing-1.jpgAnti-DRAP1 Antibody Picoband®IHC analysis of DRAP1 using anti-DRAP1 antibody (A08536). DRAP1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DRAP1 Antibody (A08536) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08536-drap1-primary-antibodies-ihc-testing-2.jpgAnti-DRAP1 Antibody Picoband®IHC analysis of DRAP1 using anti-DRAP1 antibody (A08536). DRAP1 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DRAP1 Antibody (A08536) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08536-drap1-primary-antibodies-ihc-testing-3.jpgAnti-DRAP1 Antibody Picoband®IHC analysis of DRAP1 using anti-DRAP1 antibody (A08536). DRAP1 was detected in a paraffin-embedded section of rat BRAIN tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DRAP1 Antibody (A08536) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08536-drap1-primary-antibodies-if-testing-1.jpgAnti-DRAP1 Antibody Picoband®IF analysis of DRAP1 using anti-DRAP1 antibody (A08536) and anti-Alpha Tubulin antibody (M03989-3). DRAP1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DRAP1 Antibody (A08536) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08536-drap1-primary-antibodies-ip-testing-1.jpgAnti-DRAP1 Antibody Picoband®Immunoprecipitating DRAP1 in U251 whole cell lysate. Western blot analysis of DRAP1 using anti-DRAP1 antibody (A08536). Lane 1: U251 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DRAP1 antibody in U251 whole cell lysate, Lane 3: anti-DRAP1 antibody (2μg) + U251 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DRAP1 antigen affinity purified polyclonal antibody (A08536) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DRAP1 at approximately 26 kDa. The expected band size for DRAP1 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08536-drap1-primary-antibodies-fcm-testing-1.jpgAnti-DRAP1 Antibody Picoband®Flow Cytometry analysis of PC-3 cells using anti-DRAP1 antibody (A08536). Overlay histogram showing PC-3 cells stained with A08536 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DRAP1 Antibody (A08536, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1332282026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332292026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332302026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332312026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332322026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332332026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332342026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332352026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332362026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332372026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08406-1-dusp12-primary-antibodies-wb-testing-1.jpgAnti-DUSP12 Antibody Picoband®Western blot analysis of DUSP12 using anti-DUSP12 antibody (A08406-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP12 antigen affinity purified polyclonal antibody (A08406-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DUSP12 at approximately 36 kDa. The expected band size for DUSP12 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08406-1-dusp12-primary-antibodies-if-testing-1.jpgAnti-DUSP12 Antibody Picoband®IF analysis of DUSP12 using anti-DUSP12 antibody (A08406-1) and anti-Alpha Tubulin antibody (M03989-3). DUSP12 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DUSP12 Antibody (A08406-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08406-1-dusp12-primary-antibodies-fcm-testing-1.jpgAnti-DUSP12 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-DUSP12 antibody (A08406-1). Overlay histogram showing SH-SY5Y cells stained with A08406-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DUSP12 Antibody (A08406-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1332382026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1332392026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-actin-antibody-monoclonal-m01263-9-boster.html2026-03-16T05:11:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-9-12951_2025_3750_fig4_html.pngAnti-Beta-Actin Actb Antibody (Monoclonal, 31A06)Evaluation of in vitro brain targeting and intracellular ROS scavenging. ( A ) Schematic illustration of the transwell-based in vitro BBB model. ( B ) Quantitative evaluation of BBB permeability using the apparent permeability coefficient. ( C ) Western blot analysis of CD44 protein levels. ( D ) TEER values of the bEnd.3 cell monolayer before and after treatment. ( E ) Fluorescence microscopy images of H 2 O 2 -induced oxidative stress model after being treated with LAbs@Lip@Rapa, Lip@BMn/Rapa, and LAbs@Lip@BMn/Rapa. Scale bar: 100 μm. ( F and G ) Intracellular oxygen generation in BV2 and HT22 cells detected by the (Ru(dpp) 3 )]Cl 2 fluorescence probe. The red fluorescence was quenched in the presence of molecular oxygen. Scale bar: 100 μm. Data are presented as mean ± standard deviation ( n = 3). *** P < 0.001 and **** P < 0.0001. Abbreviations: A: Free C6; B: C6-Lip@BMn/Rapa; C: C6-KD-LAbs@Lip@BMn/Rapa; D: C6-LAbs@Lip@BMn/Rapa Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-025-03750-y'>41068796</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-9-41467_2025_63749_fig4_html.pngAnti-Beta-Actin Actb Antibody (Monoclonal, 31A06)Validation of the alleviating hypoxia capacity and anti-apoptotic effects of photosynthetic system on cardiomyocytes. A Bright-field and fluorescence-field images of C. pyre in HAMA, scale bar, 5 μm. B Activity of C. pyre . C Dissolved O 2 level changes of the solution containing HCU with various treatments. D Flow chart of induced hypoxia in cardiomyocytes. E Fluorescence images of H9c2 cells staining with RDPP probe after various treatments. F Fluorescence images of H9c2 cells staining with HIF-1α antibody (red) and DAPI (blue) after various treatments, scale bar: 50 μm. G Quantitative fluorescence analysis of RDPP probes ( n = 5, n represent the number of independent experiments, mean ± s.d.). H Quantitative fluorescence analysis of HIF-1α ( n = 5, n represent the number of independent experiments, mean ± s.d.). I The levels of proteins including HIF-1α, Bcl-2, C-Caspase 3 and Cyt c in H9c2 cells analyzed by western blotting. J – M Corresponding quantitative histograms of protein expression ( n = 5, n represent the number of independent experiments, mean ± s.d.). N Anti-apoptosis analysis of H9c2 cells by flow cytometry after various treatments. O Corresponding quantitative histograms of anti-apoptosis rate ( n = 3, n represent the number of independent experiments, mean ± s.d.). Statistical analysis was performed using unpaired two-tailed Student’s t test. Independent experiments were performed ( n = 3) for ( A ) with similar results. Source data are provided as a Source Data file. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-63749-9'>41028003</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-9-41467_2025_64480_fig1_html.pngAnti-Beta-Actin Actb Antibody (Monoclonal, 31A06)Upregulation of PGK1 and 3-PG in mouse models and human biopsies induced by AKI. For the I/R mouse model, the renal pedicles were clamped with microaneurysm clips for 30 min. After clip removal, the mice were placed in a 37 °C incubator until fully awake. After 24 h, the mice were re-anesthetized with 5% isoflurane, anesthesia was confirmed by a tail pinch, and they were sacrificed by cervical dislocation. In the cisplatin-induced AKI mice, 8-week-old C57BL/6J mice were injected with cisplatin (20 mg/kg) dissolved in saline, while the control mice were injected with the same amount of saline. In the LPS-induced AKI mice, 8-week-old C57BL/6J mice received an intraperitoneal injection of LPS at a dose of 10 mg/kg. After 3 days of cisplatin treatment and 24 h of LPS treatment, the mice were re-anesthetized with 5% isoflurane, anesthesia was confirmed by a tail pinch, and they were sacrificed by cervical dislocation. Kidneys and blood samples were collected and stored at −80 °C. Serum samples were obtained by centrifugation at 800 g for 20 min. a Representative blots and quantitative analysis of PGK1 in the kidneys of I/R-induced AKI mice ( n = 4 per group). b Relative mRNA level of PGK1 in kidneys of I/R-induced AKI mice ( n = 6 per group). c Immunofluorescence (upper) and immunohistochemistry (lower) of PGK1 in kidneys of I/R-induced AKI mice. Red fluorescence indicated PGK1, and Green fluorescence indicated E-cadherin. The cell nucleus was labeled with DAPI ( n = 4 per group). d Serum 3-PG levels in Sham and I/R mice ( n = 6 per group). e 3-PG levels in the kidneys of Sham and I/R mice ( n = 6 per group). f PGK1 activity in the kidneys of Sham and I/R mice ( n = 6 per group). g Representative blots and quantitative analysis of PGK1 in kidneys of cisplatin-induced AKI mice ( n = 4 per group). h Relative mRNA level of PGK1 in kidneys of cisplatin-induced AKI mice ( n = 6 per group). i Immunofluorescence (upper) and immunohistochemistry (lower) of PGK1 in kidneys of cisplatin-induced AKI mice. Red fluorescence indicated PGK1, and Green fluorescence indicated E-cadherin. The cell nucleus was labeled with DAPI ( n = 4 per group). j Serum 3-PG levels in control and cisplatin mice ( n = 6 per group). k 3-PG levels in the kidneys of control and cisplatin mice ( n = 6 per group). l PGK1 activity in the kidneys of control and cisplatin mice ( n = 6 per group). m Representative blots and quantitative analysis of PGK1 in the kidneys of LPS-induced AKI mice ( n = 4 per group). n Relative mRNA level of PGK1 in the kidneys of LPS-induced AKI mice ( n = 6 per group). o Immunofluorescence (upper) and immunohistochemistry (lower) of PGK1 in kidneys of LPS-induced AKI mice. Red fluorescence indicated PGK1, and Green fluorescence indicated E-cadherin. The cell nucleus was labeled with DAPI ( n = 4 per group). p Serum 3-PG levels in control and LPS mice ( n = 6 per group). q 3-PG levels in the kidneys of control and LPS mice ( n = 6 per group). r PGK1 activity in the kidneys of control and LPS mice ( n = 6 per group). s Representative blots and quantitative analysis of PGK1 in renal tissues from healthy controls and patients with AKI ( n = 6 per group). t Relative mRNA level of PGK1 in renal tissues from healthy controls and patients with AKI ( n = 6 per group). u Immunofluorescence (upper) and immunohistochemistry (lower) of PGK1 in renal tissues from healthy controls and patients with AKI. Red fluorescence indicated PGK1, and Green fluorescence indicated E-cadherin. The cell nucleus was labeled with DAPI ( n = 6 per group). v Serum 3-PG levels in healthy (H) controls and patients with AKI ( n = 30 per group). w Correlation analysis between peripheral blood 3-PG and BUN ( n = 30 per group). x Correlation analysis between peripheral blood 3-PG and Scr ( n = 30 per group). Data are represented as the Mean ± SEM. Statistical differences for ( a – v ) were analyzed using an unpaired two-tailed Student’s t -test, while statistical significance for ( w , x ) was determined by Pearson’s analysis. Source data are provided as a Source Data file. PGK1 phosphoglycerate kinase 1, 3-PG 3-phosphoglycerate, I/R ischemic/reperfusion, IHC immunohistochemistry, LPS lipopolysaccharide, GAPDH glyceraldehyde-3-phosphate dehydrogenase, AKI acute kidney injury, BUN blood urea nitrogen, Scr serum creatinine. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-64480-1'>41136411</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-9-41467_2025_64480_fig2_html.pngAnti-Beta-Actin Actb Antibody (Monoclonal, 31A06)RTECs-specific knockout of PGK1 protected against I/R-induced AKI. After adaptation for one week, eight-week-old PGK1 flox/flox and PGK1 CKO mice underwent renal ischemia for 30 min, followed by renal reperfusion for 24 h. The blood and kidneys were then collected for serological testing and histological examination. a BUN levels ( n = 6 per group). b Scr levels ( n = 6 per group). c Serum cystatin C levels ( n = 6 per group). d Tubular injury score ( n = 6 per group). e Quantitative analysis of DHE oxidation ( n = 6 per group). f HE staining, DHE oxidation, Nox2 immunofluorescence, TUNEL analysis, F4/80 immunofluorescence, and IL-1β immunofluorescence of renal sections ( n = 6 per group). g Quantitative analysis of Nox2 immunofluorescence ( n = 6 per group). h Quantitative analysis of TUNEL analysis ( n = 6 per group). i Quantitative analysis of F4/80 immunofluorescence ( n = 6 per group). j Quantitative analysis of IL-1β immunofluorescence ( n = 6 per group). k Relative mRNA level of IL-1β ( n = 6 per group). l Relative mRNA level of IL-6 ( n = 6 per group). m Relative mRNA level of IL-10 ( n = 6 per group). n Relative mRNA level of TNF-α ( n = 6 per group). o Relative mRNA level of 12-Lox ( n = 6 per group). p Relative mRNA level of Rac-1 ( n = 6 per group). q Quantitative analysis of NGAL ( n = 6 per group). r Representative blots of NGAL, Bax, and Bcl-2 ( n = 6 per group). s Quantitative analysis of Bax ( n = 6 per group). t Quantitative analysis of Bcl-2 ( n = 6 per group). Data are represented as the Mean ± SEM. Statistical differences for ( a – t ) were performed using one-sided ANOVA/Bonferroni test. Source data are provided as a Source Data file. RTECs renal tubular epithelial cells, BUN blood urea nitrogen, Scr serum creatinine, DHE dihydroethidium, I/R ischemic/reperfusion, HE hematoxylin-Eosin, NOX2 reduced nicotinamide adenine dinucleotide phosphate oxidase 2, TUNEL terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, IL-1β interleukin-1β, IL-6 interleukin-6, IL-10 interleukin-10, TNF-α tumor necrosis factor α, 12-Lox 12-lipoxygenase, Rac-1 Ras-related C3 botulinum toxin substrate 1, NGAL neutrophil gelatinase-associated lipocalin, Bax Bcl-2-associated X protein, Bcl-2, B-cell lymphoma/leukemia-2 gene, CKO conditional knockout. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-64480-1'>41136411</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-9-41467_2025_64480_fig3_html.pngAnti-Beta-Actin Actb Antibody (Monoclonal, 31A06)3-PG infusion worsened I/R-induced AKI. After adaptation for one week, eight-week-old C57BL/6J mice were intraperitoneally injected with 3-PG (30 mg/kg) for 2 consecutive weeks before undergoing renal I/R surgery. After renal reperfusion for 24 h, the blood and kidneys were then collected for serological testing and histological examination. a BUN levels ( n = 6 per group). b Scr levels ( n = 6 per group). c Serum cystatin C levels ( n = 6 per group). d Tubular injury score ( n = 6 per group). e Quantitative analysis of DHE oxidation ( n = 6 per group). f HE staining, DHE oxidation, Nox2 immunofluorescence, TUNEL analysis, F4/80 immunofluorescence, IL-1β immunofluorescence of renal sections ( n = 6 per group). g Quantitative analysis of Nox2 immunofluorescence ( n = 6 per group). h Quantitative analysis of TUNEL analysis ( n = 6 per group). i Quantitative analysis of F4/80 immunofluorescence ( n = 6 per group). j Quantitative analysis of IL-1β immunofluorescence ( n = 6 per group). k Relative mRNA level of IL-1β ( n = 6 per group). l Relative mRNA level of IL-6 ( n = 6 per group). m Relative mRNA level of IL-10 ( n = 6 per group). n Relative mRNA level of TNF-α ( n = 6 per group). o Relative mRNA level of 12-Lox ( n = 6 per group). p Relative mRNA level of Rac-1 ( n = 6 per group). q Quantitative analysis of NGAL ( n = 6 per group). r Quantitative analysis of Bax ( n = 6 per group). s Quantitative analysis of Bcl-2 ( n = 6 per group). t Representative blots of NGAL, Bax, and Bcl-2 ( n = 6 per group). Data are represented as the Mean ± SEM. Statistical differences for ( a – t ) were performed using one-sided ANOVA/Bonferroni test. Source data are provided as a Source Data file. 3-PG 3-phosphoglycerate, I/R ischemic/reperfusion, BUN blood urea nitrogen, Scr serum creatinine, DHE dihydroethidium, HE hematoxylin-Eosin, NOX2 reduced nicotinamide adenine dinucleotide phosphate oxidase 2, TUNEL terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, IL-1β interleukin-1β, IL-6 interleukin-6, IL-10 interleukin-10, TNF-α tumor necrosis factor α, 12-Lox 12-lipoxygenase, Rac-1 Ras-related C3 botulinum toxin substrate 1, NGAL neutrophil gelatinase-associated lipocalin, Bax Bcl-2-associated X protein, Bcl-2 B-cell lymphoma/leukemia-2 gene. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-64480-1'>41136411</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-9-41467_2025_64480_fig4_html.pngAnti-Beta-Actin Actb Antibody (Monoclonal, 31A06)RNA sequencing reveals that PGK1 activation leads to ferroptosis in AKI by upregulating ALOX12. a , b Heatmap showing the differentially expressed gene expression in AKI mice after PGK1 knockout ( n = 3 per group). c Volcano plot showing the differentially expressed gene expression in AKI mice after PGK1 knockout ( n = 3 per group). Differential expression analysis was performed using a two-sided statistical test, and p -values were adjusted for multiple comparisons using the Benjamini–Hochberg method to control the false discovery rate (FDR). d KEGG analysis of differentially expressed genes ( n = 3 per group). Enrichment significance was evaluated using a two-sided hypergeometric test, with Benjamini–Hochberg correction applied for multiple comparisons. e , g Representative blots and quantitative analysis of ALOX12 in kidneys of I/R-induced AKI mice ( n = 4 per group). f , h Representative blots and quantitative analysis of ALOX12 in H/R-induced RTECs ( n = 4 per group). i After adaptation for one week, eight-week-old PGK1 flox/flox and PGK1 CKO mice underwent renal ischemia for 30 min, followed by renal reperfusion for 24 h. The kidneys were then collected for histological examination. Prussian staining, FerroOrange staining, and Liperfluo staining were obtained ( n = 6 per group). j Quantitative analysis of FerroOrange staining ( n = 6 per group). k Quantitative analysis of Liperfluo staining ( n = 6 per group). l Relative mRNA level of PTGS2 ( n = 6 per group). m , n Representative blots and quantitative analysis of ALOX12 ( n = 6 per group). Data are represented as the Mean ± SEM. Statistical differences for g-h were analyzed using an unpaired two-tailed Student’s t -test. Statistical differences for ( j – m ) were performed using one-sided ANOVA/Bonferroni test. Source data are provided as a Source Data file. PGK1 phosphoglycerate kinase 1, CKO conditional knockout, ALOX12 arachidonate 12-lipoxygenase, I/R ischemic/reperfusion, H/R hypoxia/reoxygenation, PTGS2 prostaglandin-endoperoxide synthase 2. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-64480-1'>41136411</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-9-41467_2025_64480_fig5_html.pngAnti-Beta-Actin Actb Antibody (Monoclonal, 31A06)Interaction of PGK1 with PKM2 induces dimerization and nuclear translocation of PKM2. a Ion diagram of PKM2 identified by co-IP MS. b Laser confocal photography showing the direct interaction of PGK1 with PKM2 ( n = 3 per group). c The co-IP assays in HEK293 cells transfected with Flag-tagged PGK1 and HA-tagged PKM2 ( n = 3 per group). d The co-IP assays in HEK293 cells transfected with Flag-tagged PKM2 and HA-tagged PGK1 ( n = 3 per group). e , f GST precipitation assays showing direct PGK1-PKM2 binding. Purified GST was used as a control ( n = 3 per group). g – l Representative blots and quantitative analysis of PKM2, P-PKM2, dimeric forms, cytoplasmic PKM2, and nuclear PKM2 ( n = 4 per group). Data are represented as the Mean ± SEM. Statistical differences for ( h – l ) were performed using one-sided ANOVA/Bonferroni test. Source data are provided as a Source Data file. PGK1 phosphoglycerate kinase 1, PKM2 pyruvate kinase M2, HA Hemagglutinin, GST glutathione S-transferase tag, co-IP MS co-Immunoprecipitation mass spectrometry, CKO conditional knockout. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-64480-1'>41136411</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-9-41467_2025_64480_fig6_html.pngAnti-Beta-Actin Actb Antibody (Monoclonal, 31A06)Accumulated PKM2 in the nucleus interacted with Pknox1, increasing the DNA binding of Pknox1 to Alox12 promoters. a H/R enhanced nuclear translocation of PKM2 and the interaction of PGK1 with PKM2 ( n = 3 per group). b PGK1 deficiency reduced the binding of Pknox1 to ALOX12 promoters in I/R mice ( n = 6 per group). c PGK1 deficiency reduced the binding of Pknox1 to ALOX12 promoters IN H/R-exposed HK-2 cells ( n = 6 per group). d Effects of Pknox1 knockdown on cell viability ( n = 6 per group). e PI/Hochest staining, FerroOrange staining, and Liperfluo staining ( n = 6 per group). f Quantitative analysis of PI/Hochest staining ( n = 6 per group). g Quantitative analysis of FerroOrange staining ( n = 6 per group). h Quantitative analysis of Liperfluo staining ( n = 6 per group). i , j Representative blots and quantitative analysis of ALOX12 ( n = 6 per group). k Relative mRNA level of PTGS2 ( n = 6 per group). l Knockdown of PGK1 and PKM2 inhibited the RLU of ALOX12 ( n = 6 per group). m Knockdown of PGK1 and PKM2 inhibited the binding of Pknox1 to the ALOX12 promoters ( n = 6 per group). n Relative mRNA level of Pknox1 ( n = 6 per group). o , p Representative blots and quantitative analysis of Pknox1 in HK-2 cells after knockdown of PGK1 and PKM2 ( n = 4 per group). Data are represented as the Mean ± SEM. Statistical differences for ( b – p ) were performed using one-sided ANOVA/Bonferroni test. Source data are provided as a Source Data file. PGK1 phosphoglycerate kinase 1, H/R hypoxia/reoxygenation, I/R ischemic/reperfusion, PKM2 pyruvate kinase M2, ALOX12 arachidonate 12-lipoxygenase, Pknox1 PBX/knotted1 homeobox 1, PTGS2 prostaglandin-endoperoxide synthase 2, RLU Relative luciferase unit. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-64480-1'>41136411</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-9-41598_2025_17122_fig2_html.pngAnti-Beta-Actin Actb Antibody (Monoclonal, 31A06)ALPK1 pathway and GSDMD pyroptosis pathway was induced in db/db mice retinas. ( A ) Representative photomicrographs of ALPK1-positive cells in retina of db/m and db/db mice. ( B ) Quantification of ALPK1-positive expression in retina of db/m and db/db mice (n = 4 per group). ( C ) mRNA levels of ALPK1 in the retina (n = 4 per group). ( D ) Representative immunoblots of p-TIFA, TIFA, TRAF6 and β-Actin proteins in retina of db/m and db/db mice. Quantitative analysis of ( E ) p-TIFA/TIFA ( F ) TRAF6 proteins. ( F ) Representative images of immunofluorescence staining for NF-κB p65 (red) and DAPI (blue) and NLRP3 (green) and DAPI (blue). ( G ) Quantification of NLRP3-positive expression in retina of db/m and db/db mice. ( H ) Preservative photomicrographs of Caspase-1-positive cells and GSDMD-positive cells in retina of db/m and db/db mice. ( I ) Quantification of Caspase-1-positive expression in retina of db/m and db/db mice. ( J ) Quantification of GSDMD-positive expression in retina of db/m and db/db mice (n = 4 per group). The data are expressed as mean ± SEM. * P < 0.05, ** P < 0.01, vs. db/m group. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-17122-x'>41006430</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-9-41467_2025_64480_fig7_html.pngAnti-Beta-Actin Actb Antibody (Monoclonal, 31A06)L7DG acts as an inhibitor of PGK1 that ameliorates AKI. After adaptation for one week, eight-week-old C57BL/6J mice were intraperitoneally injected with L7DG (10 mg/kg) for 3 consecutive days before undergoing renal I/R surgery. After renal reperfusion for 24 h, the blood and kidneys were then collected for serological testing and histological examination. a BUN levels ( n = 6 per group). b Scr levels ( n = 6 per group). c Serum cystatin C levels ( n = 6 per group). d HE staining, DHE oxidation, Nox2 immunofluorescence, TUNEL analysis, F4/80 immunofluorescence, IL-1β immunofluorescence of renal sections ( n = 6 per group). e Tubular injury score ( n = 6 per group). f Quantitative analysis of DHE oxidation ( n = 6 per group). g Quantitative analysis of Nox2 immunofluorescence ( n = 6 per group). h Quantitative analysis of TUNEL analysis ( n = 6 per group). i Quantitative analysis of F4/80 immunofluorescence ( n = 6 per group). j Quantitative analysis of IL-1β immunofluorescence ( n = 6 per group). k Relative mRNA level of IL-1β ( n = 6 per group). l Relative mRNA level of IL-6 ( n = 6 per group). m Relative mRNA level of IL-10 ( n = 6 per group). n Relative mRNA level of TNF-α ( n = 6 per group). o Relative mRNA level of 12-Lox ( n = 6 per group). p Relative mRNA level of Rac-1 ( n = 6 per group). q Relative mRNA level of Pknox1 ( n = 4 per group). r – v Representative blots and quantitative analysis of PKM2 ( n = 4 per group), P-PKM2 ( n = 4 per group), PGK1 ( n = 6 per group) and NGAL ( n = 6 per group). Data are represented as the Mean ± SEM. Statistical differences for ( a – u ) were performed using one-sided ANOVA/Bonferroni test. Source data are provided as a Source Data file. L7DG luteolin-7-diglucuronide, PGK1 phosphoglycerate kinase 1, BUN blood urea nitrogen, Scr serum creatinine, DHE dihydroethidium, I/R ischemic/reperfusion, HE hematoxylin-Eosin, NOX2 reduced nicotinamide adenine dinucleotide phosphate oxidase 2, TUNEL terminal deoxynucleotidyl transferase-mediated dUTP Nick-End Labeling, IL-1β interleukin-1β, IL-6 interleukin-6, IL-10 interleukin-10, TNF-α tumor necrosis factor α, 12-Lox 12-lipoxygenase, Rac-1 Ras-related C3 botulinum toxin substrate 1, Pknox1 PBX/knotted1 homeobox, PKM2 pyruvate kinase M2, NGAL neutrophil gelatinase-associated lipocalin. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-64480-1'>41136411</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-9-41598_2025_17122_fig4_html.pngAnti-Beta-Actin Actb Antibody (Monoclonal, 31A06)ALPK1 was induced in BV2 cells after hypoxia. ( A ) Morphological diagram of BV2 cells treated with hypoxia. ( B ) Survival rate of BV2 cells. ( C ) Representative immunoblots of ALPK1 and β-Actin proteins in hypoxia model of BV2 cells. Quantitative analysis of ( D ) ALPK1 proteins. The data are expressed as mean ± SEM.* P < 0.05, ** P < 0.01 vs. Control group. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-17122-x'>41006430</a>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01263-9-41598_2025_17122_fig5_html.pngAnti-Beta-Actin Actb Antibody (Monoclonal, 31A06)ALPK1 knockdown inhibited ALPK1 pathway and GSDMD pyroptosis pathway in BV2 cells induced by hypoxia. ( A ) Representative immunoblots of ALPK1, p-TIFA, TIFA and β-Actin proteins in hypoxia model of BV2 cells. Quantitative analysis of ( B ) ALPK1 ( C ) p-TIFA/TIFA, ( D ) TRAF6 proteins. ( E ) Representative images of immunofluorescence staining for NF-κB p65 (red) and DAPI (blue). ( F ) Representative immunoblots of NLRP3, c-Caspase-1, pro-Caspase-1, GSDMD-N, GSDMD and β-Actin proteins in hypoxia model of BV2 cells. Quantitative analysis of ( G ) NLRP3, ( H ) c-Caspase-1/pro-Caspase-1, ( I ) GSDMD-N/GSDMD proteins. The data are representative of three independent experiments and are expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. Control group. # P < 0.05, ## P < 0.01 vs. sgRNA-NC/Hypoxia group. Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-17122-x'>41006430</a> https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-alpha-tubulin-antibody-monoclonal-m03989-5-boster.html2026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hnf4a-picoband-antibody-aza8e5k2-boster.html2026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8e5k2-hnf4a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HNF4A Antibody Picoband®Western blot analysis of HNF4A using anti-HNF4A antibody (AZA8E5K2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF4A antigen affinity purified polyclonal antibody (AZA8E5K2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HNF4A at approximately 47 kDa. The expected band size for HNF4A is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ldha-picoband-antibody-azq9pvk5-boster.html2026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pvk5-ldha-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LDHA Antibody Picoband®Western blot analysis of LDHA using anti-LDHA antibody (AZQ9PVK5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDHA antigen affinity purified polyclonal antibody (AZQ9PVK5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LDHA at approximately 36 kDa. The expected band size for LDHA is at 26 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ldhba-picoband-antibody-azq9pvk4-boster.html2026-03-17T05:17:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pvk4-ldhba-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LDHBA Antibody Picoband®Western blot analysis of LDHBA using anti-LDHBA antibody (AZQ9PVK4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDHBA antigen affinity purified polyclonal antibody (AZQ9PVK4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LDHBA at approximately 36 kDa. The expected band size for LDHBA is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cox-1-cyclooxygenase-1-ptgs1-picoband-antibody-azq8jh44-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8jh44-ptgs1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish COX-1/Cyclooxygenase-1/PTGS1 Antibody Picoband®Western blot analysis of PTGS1 using anti-PTGS1 antibody (AZQ8JH44). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS1 antigen affinity purified polyclonal antibody (AZQ8JH44) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTGS1 at approximately 72 kDa. The expected band size for PTGS1 is at 65 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tph1a-picoband-antibody-azq6pbx4-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pbx4-tph1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TPH1A Antibody Picoband®Western blot analysis of TPH1A using anti-TPH1A antibody (AZQ6PBX4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPH1A antigen affinity purified polyclonal antibody (AZQ6PBX4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TPH1A at approximately 55 kDa. The expected band size for TPH1A is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pbx4-tph1a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish TPH1A Antibody Picoband®IHC analysis of TPH1A using anti-TPH1A antibody (AZQ6PBX4). TPH1A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TPH1A Antibody (AZQ6PBX4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ihha-picoband-antibody-aza3kns3-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza3kns3-ihha-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish IHHA Antibody Picoband®Western blot analysis of IHHA using anti-IHHA antibody (AZA3KNS3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IHHA antigen affinity purified polyclonal antibody (AZA3KNS3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IHHA at approximately 45 kDa. The expected band size for IHHA is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza3kns3-ihha-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish IHHA Antibody Picoband®IHC analysis of IHHA using anti-IHHA antibody (AZA3KNS3). IHHA was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IHHA Antibody (AZA3KNS3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-syn2a-picoband-antibody-azf1r0a8-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r0a8-syn2a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SYN2A Antibody Picoband®Western blot analysis of SYN2A using anti-SYN2A antibody (AZF1R0A8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYN2A antigen affinity purified polyclonal antibody (AZF1R0A8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYN2A at approximately 51 kDa. The expected band size for SYN2A is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r0a8-syn2a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish SYN2A Antibody Picoband®IHC analysis of SYN2A using anti-SYN2A antibody (AZF1R0A8). SYN2A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SYN2A Antibody (AZF1R0A8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-alpl-picoband-antibody-aza0a8m2bgi2-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2bgi2-alpl-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ALPL Antibody Picoband®Western blot analysis of ALPL using anti-ALPL antibody (AZA0A8M2BGI2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALPL antigen affinity purified polyclonal antibody (AZA0A8M2BGI2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALPL at approximately 58 kDa. The expected band size for ALPL is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cathepsin-k-ctsk-picoband-antibody-azq568d6-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq568d6-ctsk-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Cathepsin K/CTSK Antibody Picoband®Western blot analysis of CTSK using anti-CTSK antibody (AZQ568D6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTSK antigen affinity purified polyclonal antibody (AZQ568D6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CTSK at approximately 27,38-46 kDa. The expected band size for CTSK is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-sypa-picoband-antibody-azb0s5b9-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0s5b9-sypa-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SYPA Antibody Picoband®Western blot analysis of SYPA using anti-SYPA antibody (AZB0S5B9). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYPA antigen affinity purified polyclonal antibody (AZB0S5B9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYPA at approximately 37 kDa. The expected band size for SYPA is at 32 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cytokeratin-18-krt18-picoband-antibody-azq7zts4-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zts4-krt18-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Cytokeratin 18/KRT18 Antibody Picoband®Western blot analysis of KRT18 using anti-KRT18 antibody (AZQ7ZTS4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT18 antigen affinity purified polyclonal antibody (AZQ7ZTS4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KRT18 at approximately 48 kDa. The expected band size for KRT18 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zts4-krt18-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Cytokeratin 18/KRT18 Antibody Picoband®IHC analysis of KRT18 using anti-KRT18 antibody (AZQ7ZTS4). KRT18 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KRT18 Antibody (AZQ7ZTS4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zts4-krt18-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish Cytokeratin 18/KRT18 Antibody Picoband®IHC analysis of KRT18 using anti-KRT18 antibody (AZQ7ZTS4). KRT18 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KRT18 Antibody (AZQ7ZTS4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zts4-krt18-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish Cytokeratin 18/KRT18 Antibody Picoband®IHC analysis of KRT18 using anti-KRT18 antibody (AZQ7ZTS4). KRT18 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KRT18 Antibody (AZQ7ZTS4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fads2-picoband-antibody-azq9dex7-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9dex7-fads2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FADS2 Antibody Picoband®Western blot analysis of FADS2 using anti-FADS2 antibody (AZQ9DEX7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADS2 antigen affinity purified polyclonal antibody (AZQ9DEX7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FADS2 at approximately 52 kDa. The expected band size for FADS2 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ifng1r-picoband-antibody-azq5tle6-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5tle6-ifng1r-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish IFNG1R Antibody Picoband®Western blot analysis of IFNG1R using anti-IFNG1R antibody (AZQ5TLE6). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG1R antigen affinity purified polyclonal antibody (AZQ5TLE6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IFNG1R at approximately 22 kDa. The expected band size for IFNG1R is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5tle6-ifng1r-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish IFNG1R Antibody Picoband®IHC analysis of IFNG1R using anti-IFNG1R antibody (AZQ5TLE6). IFNG1R was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IFNG1R Antibody (AZQ5TLE6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5tle6-ifng1r-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish IFNG1R Antibody Picoband®IHC analysis of IFNG1R using anti-IFNG1R antibody (AZQ5TLE6). IFNG1R was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IFNG1R Antibody (AZQ5TLE6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-erk2-mapk1-picoband-antibody-azq7zw72-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zw72-mapk1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ERK2/MAPK1 Antibody Picoband®Western blot analysis of MAPK1 using anti-MAPK1 antibody (AZQ7ZW72). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK1 antigen affinity purified polyclonal antibody (AZQ7ZW72) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAPK1 at approximately 42,44 kDa. The expected band size for MAPK1 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tgf-beta-3-tgfb3-picoband-antibody-azq66i23-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq66i23-tgfb3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TGF Beta 3/TGFB3 Antibody Picoband®Western blot analysis of TGFB3 using anti-TGFB3 antibody (AZQ66I23). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFB3 antigen affinity purified polyclonal antibody (AZQ66I23) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TGFB3 at approximately 48 kDa. The expected band size for TGFB3 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gsr-picoband-antibody-aza0a8m9qb21-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qb21-gsr-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GSR Antibody Picoband®Western blot analysis of GSR using anti-GSR antibody (AZA0A8M9QB21). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSR antigen affinity purified polyclonal antibody (AZA0A8M9QB21) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSR at approximately 55 kDa. The expected band size for GSR is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-slc2a1a-picoband-antibody-azq285p3-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq285p3-slc2a1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish SLC2A1A Antibody Picoband®Western blot analysis of SLC2A1A using anti-SLC2A1A antibody (AZQ285P3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A1A antigen affinity purified polyclonal antibody (AZQ285P3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC2A1A at approximately 55 kDa. The expected band size for SLC2A1A is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq285p3-slc2a1a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish SLC2A1A Antibody Picoband®IHC analysis of SLC2A1A using anti-SLC2A1A antibody (AZQ285P3). SLC2A1A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC2A1A Antibody (AZQ285P3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fabp4a-picoband-antibody-azq66i80-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq66i80-fabp4a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FABP4A Antibody Picoband®Western blot analysis of FABP4A using anti-FABP4A antibody (AZQ66I80). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP4A antigen affinity purified polyclonal antibody (AZQ66I80) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FABP4A at approximately 15 kDa. The expected band size for FABP4A is at 15 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-drl-picoband-antibody-azq9w747-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9w747-drl-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DRL Antibody Picoband®Western blot analysis of DRL using anti-DRL antibody (AZQ9W747). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRL antigen affinity purified polyclonal antibody (AZQ9W747) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DRL at approximately 50 kDa. The expected band size for DRL is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-myo6a-picoband-antibody-azf1q6q6-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1q6q6-myo6a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MYO6A Antibody Picoband®Western blot analysis of MYO6A using anti-MYO6A antibody (AZF1Q6Q6). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYO6A antigen affinity purified polyclonal antibody (AZF1Q6Q6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYO6A at approximately 150 kDa. The expected band size for MYO6A is at 144 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1q6q6-myo6a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish MYO6A Antibody Picoband®IHC analysis of MYO6A using anti-MYO6A antibody (AZF1Q6Q6). MYO6A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYO6A Antibody (AZF1Q6Q6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nfkb1-p105-nfkb1-picoband-antibody-aza0a8m9qgx8-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qgx8-nfkb1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish NFKB1 p105/NFKB1 Antibody Picoband®IHC analysis of NFKB1 using anti-NFKB1 antibody (AZA0A8M9QGX8). NFKB1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NFKB1 Antibody (AZA0A8M9QGX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9qgx8-nfkb1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NFKB1 p105/NFKB1 Antibody Picoband®Western blot analysis of NFKB1 using anti-NFKB1 antibody (AZA0A8M9QGX8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFKB1 antigen affinity purified polyclonal antibody (AZA0A8M9QGX8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NFKB1 at approximately 55 kDa. The expected band size for NFKB1 is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-apaf1-picoband-antibody-azq9i9h8-boster.html2026-03-17T05:17:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i9h8-apaf1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish APAF1 AntibodyIHC analysis of APAF1 using anti-APAF1 antibody (AZQ9I9H8). APAF1 was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-APAF1 Antibody (AZQ9I9H8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-acox1-picoband-antibody-azq5xiz4-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5xiz4-acox1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish ACOX1 AntibodyIHC analysis of ACOX1 using anti-ACOX1 antibody (AZQ5XIZ4). ACOX1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACOX1 Antibody (AZQ5XIZ4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-trpv6-picoband-antibody-azq6jqn0-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6jqn0-trpv6-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TRPV6 Antibody Picoband®Western blot analysis of TRPV6 using anti-TRPV6 antibody (AZQ6JQN0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV6 antigen affinity purified polyclonal antibody (AZQ6JQN0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRPV6 at approximately 95 kDa. The expected band size for TRPV6 is at 80 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gnat1-picoband-antibody-azq90wx6-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90wx6-gnat1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GNAT1 Antibody Picoband®Western blot analysis of GNAT1 using anti-GNAT1 antibody (AZQ90WX6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAT1 antigen affinity purified polyclonal antibody (AZQ90WX6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GNAT1 at approximately 39 kDa. The expected band size for GNAT1 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90wx6-gnat1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish GNAT1 Antibody Picoband®IHC analysis of GNAT1 using anti-GNAT1 antibody (AZQ90WX6). GNAT1 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNAT1 Antibody (AZQ90WX6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90wx6-gnat1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish GNAT1 Antibody Picoband®IHC analysis of GNAT1 using anti-GNAT1 antibody (AZQ90WX6). GNAT1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNAT1 Antibody (AZQ90WX6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gpx4a-picoband-antibody-aza0a8m1n2t9-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1n2t9-gpx4a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GPX4A Antibody Picoband®Western blot analysis of GPX4A using anti-GPX4A antibody (AZA0A8M1N2T9). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX4A antigen affinity purified polyclonal antibody (AZA0A8M1N2T9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GPX4A at approximately 19 kDa. The expected band size for GPX4A is at 21 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lepb-picoband-antibody-azc4wyh6-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azc4wyh6-lepb-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LEPB Antibody Picoband®Western blot analysis of LEPB using anti-LEPB antibody (AZC4WYH6). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LEPB antigen affinity purified polyclonal antibody (AZC4WYH6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LEPB at approximately 19 kDa. The expected band size for LEPB is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azc4wyh6-lepb-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish LEPB Antibody Picoband®IHC analysis of LEPB using anti-LEPB antibody (AZC4WYH6). LEPB was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LEPB Antibody (AZC4WYH6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-egln1a-picoband-antibody-aze7f518-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f518-egln1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish EGLN1A Antibody Picoband®Western blot analysis of EGLN1A using anti-EGLN1A antibody (AZE7F518). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGLN1A antigen affinity purified polyclonal antibody (AZE7F518) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EGLN1A at approximately 50 kDa. The expected band size for EGLN1A is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ptena-picoband-antibody-azq6pc66-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pc66-ptena-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PTENA Antibody Picoband®Western blot analysis of PTENA using anti-PTENA antibody (AZQ6PC66). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTENA antigen affinity purified polyclonal antibody (AZQ6PC66) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTENA at approximately 60 kDa. The expected band size for PTENA is at 52 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lama2-picoband-antibody-azg8ihu0-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azg8ihu0-lama2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LAMA2 Antibody Picoband®Western blot analysis of LAMA2 using anti-LAMA2 antibody (AZG8IHU0). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMA2 antigen affinity purified polyclonal antibody (AZG8IHU0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LAMA2 at approximately 200 kDa. The expected band size for LAMA2 is at 338 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-trpm2-picoband-antibody-aza0a0r4imy7-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4imy7-trpm2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish TRPM2 AntibodyIHC analysis of TRPM2 using anti-TRPM2 antibody (AZA0A0R4IMY7). TRPM2 was detected in a paraffin-embedded section of zebrafish integument tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRPM2 Antibody (AZA0A0R4IMY7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pklr-picoband-antibody-aza0a8m9nz14-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9nz14-pklr-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PKLR Antibody Picoband®Western blot analysis of PKLR using anti-PKLR antibody (AZA0A8M9NZ14). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKLR antigen affinity purified polyclonal antibody (AZA0A8M9NZ14) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PKLR at approximately 57 kDa. The expected band size for PKLR is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-wnt4a-picoband-antibody-azp47793-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp47793-wnt4a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish WNT4A Antibody Picoband®Western blot analysis of WNT4A using anti-WNT4A antibody (AZP47793). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT4A antigen affinity purified polyclonal antibody (AZP47793) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for WNT4A at approximately 39 kDa. The expected band size for WNT4A is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-g6pd-picoband-antibody-aza0a8m2b959-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2b959-g6pd-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish G6PD Antibody Picoband®Western blot analysis of G6PD using anti-G6PD antibody (AZA0A8M2B959). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G6PD antigen affinity purified polyclonal antibody (AZA0A8M2B959) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for G6PD at approximately 63 kDa. The expected band size for G6PD is at 59 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lamb1a-picoband-antibody-azq8jhv7-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8jhv7-lamb1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LAMB1A Antibody Picoband®Western blot analysis of LAMB1A using anti-LAMB1A antibody (AZQ8JHV7). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMB1A antigen affinity purified polyclonal antibody (AZQ8JHV7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LAMB1A at approximately 220 kDa. The expected band size for LAMB1A is at 197 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cyp1c2-picoband-antibody-azb0jzn5-boster.html2026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0jzn5-cyp1c2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CYP1C2 Antibody Picoband®Western blot analysis of CYP1C2 using anti-CYP1C2 antibody (AZB0JZN5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1C2 antigen affinity purified polyclonal antibody (AZB0JZN5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CYP1C2 at approximately 60 kDa. The expected band size for CYP1C2 is at 51 kDa. https://www.bosterbio.com/catalog/product/view/id/1332782026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06095-2-srsf5-primary-antibodies-wb-testing-1.jpgAnti-SFRS5/SRSF5 Antibody Picoband®Western blot analysis of SRSF5 using anti-SRSF5 antibody (A06095-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRSF5 antigen affinity purified polyclonal antibody (A06095-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SRSF5 at approximately 38 kDa. The expected band size for SRSF5 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06095-2-srsf5-primary-antibodies-ihc-testing-1.jpgAnti-SFRS5/SRSF5 Antibody Picoband®IHC analysis of SRSF5 using anti-SRSF5 antibody (A06095-2). SRSF5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF5 Antibody (A06095-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06095-2-srsf5-primary-antibodies-ihc-testing-2.jpgAnti-SFRS5/SRSF5 Antibody Picoband®IHC analysis of SRSF5 using anti-SRSF5 antibody (A06095-2). SRSF5 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF5 Antibody (A06095-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06095-2-srsf5-primary-antibodies-ihc-testing-3.jpgAnti-SFRS5/SRSF5 Antibody Picoband®IHC analysis of SRSF5 using anti-SRSF5 antibody (A06095-2). SRSF5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF5 Antibody (A06095-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06095-2-srsf5-primary-antibodies-ihc-testing-4.jpgAnti-SFRS5/SRSF5 Antibody Picoband®IHC analysis of SRSF5 using anti-SRSF5 antibody (A06095-2). SRSF5 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SRSF5 Antibody (A06095-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1332792026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10242-1-slc2a11-primary-antibodies-ihc-testing-1.jpgAnti-SLC2A11 AntibodyIHC analysis of SLC2A11 using anti-SLC2A11 antibody (A10242-1). SLC2A11 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC2A11 Antibody (A10242-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1332802026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06745-1-bnc2-primary-antibodies-wb-testing-1.jpgAnti-BNC2 Antibody Picoband®Western blot analysis of BNC2 using anti-BNC2 antibody (A06745-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: mouse ovary tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BNC2 antigen affinity purified polyclonal antibody (A06745-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BNC2 at approximately 150 kDa. The expected band size for BNC2 is at 122 kDa. https://www.bosterbio.com/catalog/product/view/id/1332812026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10700-1-st3gal2-primary-antibodies-wb-testing-1.jpgAnti-ST3GAL2 Antibody Picoband®Western blot analysis of ST3GAL2 using anti-ST3GAL2 antibody (A10700-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST3GAL2 antigen affinity purified polyclonal antibody (A10700-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ST3GAL2 at approximately 45 kDa. The expected band size for ST3GAL2 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10700-1-st3gal2-primary-antibodies-ihc-testing-1.jpgAnti-ST3GAL2 Antibody Picoband®IHC analysis of ST3GAL2 using anti-ST3GAL2 antibody (A10700-1). ST3GAL2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ST3GAL2 Antibody (A10700-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10700-1-st3gal2-primary-antibodies-ihc-testing-2.jpgAnti-ST3GAL2 Antibody Picoband®IHC analysis of ST3GAL2 using anti-ST3GAL2 antibody (A10700-1). ST3GAL2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ST3GAL2 Antibody (A10700-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1332822026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07227-2-prkar1b-primary-antibodies-wb-testing-1_1.jpgAnti-PRKAR1B Antibody Picoband®Western blot analysis of PRKAR1B using anti-PRKAR1B antibody (A07227-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat restis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 5: mouse restis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAR1B antigen affinity purified polyclonal antibody (A07227-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRKAR1B at approximately 45-50 kDa. The expected band size for PRKAR1B is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07227-2-prkar1b-primary-antibodies-ihc-testing-1.jpgAnti-PRKAR1B Antibody Picoband®IHC analysis of PRKAR1B using anti-PRKAR1B antibody (A07227-2). PRKAR1B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRKAR1B Antibody (A07227-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07227-2-prkar1b-primary-antibodies-ihc-testing-2.jpgAnti-PRKAR1B Antibody Picoband®IHC analysis of PRKAR1B using anti-PRKAR1B antibody (A07227-2). PRKAR1B was detected in a paraffin-embedded section of human cerebral cortex tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRKAR1B Antibody (A07227-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07227-2-prkar1b-primary-antibodies-ihc-testing-3.jpgAnti-PRKAR1B Antibody Picoband®IHC analysis of PRKAR1B using anti-PRKAR1B antibody (A07227-2). PRKAR1B was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRKAR1B Antibody (A07227-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07227-2-prkar1b-primary-antibodies-ihc-testing-4.jpgAnti-PRKAR1B Antibody Picoband®IHC analysis of PRKAR1B using anti-PRKAR1B antibody (A07227-2). PRKAR1B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRKAR1B Antibody (A07227-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07227-2-prkar1b-primary-antibodies-ihc-testing-5.jpgAnti-PRKAR1B Antibody Picoband®IHC analysis of PRKAR1B using anti-PRKAR1B antibody (A07227-2). PRKAR1B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRKAR1B Antibody (A07227-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1332832026-03-17T05:17:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06768-3-slc15a4-primary-antibodies-ihc-testing-1.jpgAnti-SLC15A4 AntibodyIHC analysis of SLC15A4 using anti-SLC15A4 antibody (A06768-3). SLC15A4 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC15A4 Antibody (A06768-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1332842026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11977-1-slc24a3-primary-antibodies-wb-testing-1.jpgAnti-SLC24A3 Antibody Picoband®Western blot analysis of SLC24A3 using anti-SLC24A3 antibody (A11977-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC24A3 antigen affinity purified polyclonal antibody (A11977-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC24A3 at approximately 68 kDa. The expected band size for SLC24A3 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11977-1-slc24a3-primary-antibodies-ihc-testing-1.jpgAnti-SLC24A3 Antibody Picoband®IHC analysis of SLC24A3 using anti-SLC24A3 antibody (A11977-1). SLC24A3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC24A3 Antibody (A11977-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1332852026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01375-2-slco1b1-primary-antibodies-wb-testing-1.jpgAnti-SLCO1B1 Antibody Picoband®Western blot analysis of SLCO1B1 using anti-SLCO1B1 antibody (A01375-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLCO1B1 antigen affinity purified polyclonal antibody (A01375-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLCO1B1 at approximately 100 kDa. The expected band size for SLCO1B1 is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01375-2-slco1b1-primary-antibodies-if-testing-1.jpgAnti-SLCO1B1 Antibody Picoband®IF analysis of SLCO1B1 using anti-SLCO1B1 antibody (A01375-2). SLCO1B1 was detected in a paraffin-embedded section of Hela tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLCO1B1 Antibody (A01375-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01375-2-slco1b1-primary-antibodies-fcm-testing-1.jpgAnti-SLCO1B1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-SLCO1B1 antibody (A01375-2). Overlay histogram showing HepG2 cells stained with A01375-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLCO1B1 Antibody (A01375-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1332862026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07043-4-raly-primary-antibodies-wb-testing-1.jpgAnti-RALY Antibody Picoband®Western blot analysis of RALY using anti-RALY antibody (A07043-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALY antigen affinity purified polyclonal antibody (A07043-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RALY at approximately 30-38 kDa. The expected band size for RALY is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07043-4-raly-primary-antibodies-ihc-testing-1.jpgAnti-RALY Antibody Picoband®IHC analysis of RALY using anti-RALY antibody (A07043-4). RALY was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RALY Antibody (A07043-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07043-4-raly-primary-antibodies-ihc-testing-2.jpgAnti-RALY Antibody Picoband®IHC analysis of RALY using anti-RALY antibody (A07043-4). RALY was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RALY Antibody (A07043-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07043-4-raly-primary-antibodies-if-testing-1.jpgAnti-RALY Antibody Picoband®IF analysis of RALY using anti-RALY antibody (A07043-4) and anti-Alpha Tubulin antibody (M03989-3). RALY was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RALY Antibody (A07043-4) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07043-4-raly-primary-antibodies-fcm-testing-1.jpgAnti-RALY Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-RALY antibody (A07043-4). Overlay histogram showing Jurkat cells stained with A07043-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RALY Antibody (A07043-4, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1332872026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05281-1-rnaset2-primary-antibodies-wb-testing-1.jpgAnti-RNASET2 Antibody Picoband®Western blot analysis of RNASET2 using anti-RNASET2 antibody (A05281-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNASET2 antigen affinity purified polyclonal antibody (A05281-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RNASET2 at approximately 45 kDa. The expected band size for RNASET2 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05281-1-rnaset2-primary-antibodies-ihc-testing-1.jpgAnti-RNASET2 Antibody Picoband®IHC analysis of RNASET2 using anti-RNASET2 antibody (A05281-1). RNASET2 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNASET2 Antibody (A05281-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05281-1-rnaset2-primary-antibodies-ihc-testing-2.jpgAnti-RNASET2 Antibody Picoband®IHC analysis of RNASET2 using anti-RNASET2 antibody (A05281-1). RNASET2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RNASET2 Antibody (A05281-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1332882026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04841-2-prkag3-primary-antibodies-wb-testing-1.jpgAnti-PRKAG3 Antibody Picoband®Western blot analysis of PRKAG3 using anti-PRKAG3 antibody (A04841-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAG3 antigen affinity purified polyclonal antibody (A04841-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRKAG3 at approximately 54 kDa. The expected band size for PRKAG3 is at 54 kDa. https://www.bosterbio.com/catalog/product/view/id/1332892026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11330-1-psmb3-primary-antibodies-wb-testing-1.jpgAnti-PSMB3 Antibody Picoband®Western blot analysis of PSMB3 using anti-PSMB3 antibody (A11330-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB3 antigen affinity purified polyclonal antibody (A11330-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMB3 at approximately 24 kDa. The expected band size for PSMB3 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11330-1-psmb3-primary-antibodies-ihc-testing-1.jpgAnti-PSMB3 Antibody Picoband®IHC analysis of PSMB3 using anti-PSMB3 antibody (A11330-1). PSMB3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMB3 Antibody (A11330-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11330-1-psmb3-primary-antibodies-ihc-testing-2.jpgAnti-PSMB3 Antibody Picoband®IHC analysis of PSMB3 using anti-PSMB3 antibody (A11330-1). PSMB3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMB3 Antibody (A11330-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11330-1-psmb3-primary-antibodies-if-testing-1.jpgAnti-PSMB3 Antibody Picoband®IF analysis of PSMB3 using anti-PSMB3 antibody (A11330-1). PSMB3 was detected in a paraffin-embedded section of A549 tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSMB3 Antibody (A11330-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1332902026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16597-1-slc25a34-primary-antibodies-wb-testing-1.jpgAnti-SLC25A34 Antibody Picoband®Western blot analysis of SLC25A34 using anti-SLC25A34 antibody (A16597-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A34 antigen affinity purified polyclonal antibody (A16597-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A34 at approximately 32 kDa. The expected band size for SLC25A34 is at 32 kDa. https://www.bosterbio.com/catalog/product/view/id/1332912026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04563-1-rbbp5-primary-antibodies-wb-testing-1.jpgAnti-RBBP5 Antibody Picoband®Western blot analysis of RBBP5 using anti-RBBP5 antibody (A04563-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBBP5 antigen affinity purified polyclonal antibody (A04563-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBBP5 at approximately 59,66 kDa. The expected band size for RBBP5 is at 59 kDa. https://www.bosterbio.com/catalog/product/view/id/1332922026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13166-1-rbm23-primary-antibodies-wb-testing-1.jpgAnti-RBM23 Antibody Picoband®Western blot analysis of RBM23 using anti-RBM23 antibody (A13166-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM23 antigen affinity purified polyclonal antibody (A13166-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBM23 at approximately 49 kDa. The expected band size for RBM23 is at 49 kDa. https://www.bosterbio.com/catalog/product/view/id/1332932026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10082-2-pop4-primary-antibodies-wb-testing-1.jpgAnti-POP4 Antibody Picoband®Western blot analysis of POP4 using anti-POP4 antibody (A10082-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POP4 antigen affinity purified polyclonal antibody (A10082-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POP4 at approximately 26 kDa. The expected band size for POP4 is at 25 kDa. https://www.bosterbio.com/catalog/product/view/id/1332942026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11828-1-samd8-primary-antibodies-wb-testing-1.jpgAnti-SAMD8 Antibody Picoband®Western blot analysis of SAMD8 using anti-SAMD8 antibody (A11828-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAMD8 antigen affinity purified polyclonal antibody (A11828-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SAMD8 at approximately 48 kDa. The expected band size for SAMD8 is at 48 kDa. https://www.bosterbio.com/catalog/product/view/id/1332952026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09259-1-rhoj-primary-antibodies-wb-testing-1.jpgAnti-RHOJ Antibody Picoband®Western blot analysis of RHOJ using anti-RHOJ antibody (A09259-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHOJ antigen affinity purified polyclonal antibody (A09259-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RHOJ at approximately 24 kDa. The expected band size for RHOJ is at 24 kDa. https://www.bosterbio.com/catalog/product/view/id/1332962026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10990-2-sf3b6-primary-antibodies-wb-testing-1.jpgAnti-SF3B14/SF3B6 Antibody Picoband®Western blot analysis of SF3B6 using anti-SF3B6 antibody (A10990-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF3B6 antigen affinity purified polyclonal antibody (A10990-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SF3B6 at approximately 17 kDa. The expected band size for SF3B6 is at 15 kDa. https://www.bosterbio.com/catalog/product/view/id/1332972026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01662-2-sh2d1a-primary-antibodies-wb-testing-1.jpgAnti-SH2D1A Antibody Picoband®Western blot analysis of SH2D1A using anti-SH2D1A antibody (A01662-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH2D1A antigen affinity purified polyclonal antibody (A01662-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SH2D1A at approximately 16 kDa. The expected band size for SH2D1A is at 14 kDa. https://www.bosterbio.com/catalog/product/view/id/1332982026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09040-1-pou2f3-primary-antibodies-wb-testing-1.jpgAnti-POU2F3 Antibody Picoband®Western blot analysis of POU2F3 using anti-POU2F3 antibody (A09040-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: mouse skin tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POU2F3 antigen affinity purified polyclonal antibody (A09040-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POU2F3 at approximately 60 kDa. The expected band size for POU2F3 is at 47 kDa. https://www.bosterbio.com/catalog/product/view/id/1332992026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12610-1-pou6f1-primary-antibodies-wb-testing-1.jpgAnti-POU6F1 Antibody Picoband®Western blot analysis of POU6F1 using anti-POU6F1 antibody (A12610-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POU6F1 antigen affinity purified polyclonal antibody (A12610-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POU6F1 at approximately 70 kDa. The expected band size for POU6F1 is at 63 kDa. https://www.bosterbio.com/catalog/product/view/id/1333002026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06658-2-ppp1r3c-primary-antibodies-wb-testing-1.jpgAnti-PPP1R3C Antibody Picoband®Western blot analysis of PPP1R3C using anti-PPP1R3C antibody (A06658-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat H9C2(2-1) whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R3C antigen affinity purified polyclonal antibody (A06658-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPP1R3C at approximately 38 kDa. The expected band size for PPP1R3C is at 36 kDa. https://www.bosterbio.com/catalog/product/view/id/1333012026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05454-1-prcc-primary-antibodies-wb-testing-1.jpgAnti-PRCC Antibody Picoband®Western blot analysis of PRCC using anti-PRCC antibody (A05454-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRCC antigen affinity purified polyclonal antibody (A05454-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRCC at approximately 50 kDa. The expected band size for PRCC is at 52 kDa. https://www.bosterbio.com/catalog/product/view/id/1333022026-03-17T05:17:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03083-3-sgo1-primary-antibodies-wb-testing-1.jpgAnti-SGOL1/SGO1 Antibody Picoband®Western blot analysis of SGO1 using anti-SGO1 antibody (A03083-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGO1 antigen affinity purified polyclonal antibody (A03083-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SGO1 at approximately 75 kDa. The expected band size for SGO1 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03083-3-sgo1-primary-antibodies-ihc-testing-1.jpgAnti-SGOL1/SGO1 Antibody Picoband®IHC analysis of SGO1 using anti-SGO1 antibody (A03083-3). SGO1 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SGO1 Antibody (A03083-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03083-3-sgo1-primary-antibodies-ihc-testing-2.jpgAnti-SGOL1/SGO1 Antibody Picoband®IHC analysis of SGO1 using anti-SGO1 antibody (A03083-3). SGO1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SGO1 Antibody (A03083-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03083-3-sgo1-primary-antibodies-fcm-testing-1.jpgAnti-SGOL1/SGO1 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-SGO1 antibody (A03083-3). Overlay histogram showing Jurkat cells stained with A03083-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SGO1 Antibody (A03083-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333032026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02109-2-shb-primary-antibodies-wb-testing-1.jpgAnti-SHB Antibody Picoband®Western blot analysis of SHB using anti-SHB antibody (A02109-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat H9C2(2-1) whole cell lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHB antigen affinity purified polyclonal antibody (A02109-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SHB at approximately 60 kDa. The expected band size for SHB is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02109-2-shb-primary-antibodies-ihc-testing-1.jpgAnti-SHB Antibody Picoband®IHC analysis of SHB using anti-SHB antibody (A02109-2). SHB was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHB Antibody (A02109-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02109-2-shb-primary-antibodies-ihc-testing-2.jpgAnti-SHB Antibody Picoband®IHC analysis of SHB using anti-SHB antibody (A02109-2). SHB was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHB Antibody (A02109-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02109-2-shb-primary-antibodies-ihc-testing-3.jpgAnti-SHB Antibody Picoband®IHC analysis of SHB using anti-SHB antibody (A02109-2). SHB was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHB Antibody (A02109-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02109-2-shb-primary-antibodies-ihc-testing-4.jpgAnti-SHB Antibody Picoband®IHC analysis of SHB using anti-SHB antibody (A02109-2). SHB was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHB Antibody (A02109-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02109-2-shb-primary-antibodies-if-testing-1.jpgAnti-SHB Antibody Picoband®IF analysis of SHB using anti-SHB antibody (A02109-2). SHB was detected in a paraffin-embedded section of A549 tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SHB Antibody (A02109-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1333042026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03547-2-rap2a-primary-antibodies-wb-testing-1.jpgAnti-RAP2A Antibody Picoband®Western blot analysis of RAP2A using anti-RAP2A antibody (A03547-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP2A antigen affinity purified polyclonal antibody (A03547-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAP2A at approximately 18 kDa. The expected band size for RAP2A is at 21 kDa. https://www.bosterbio.com/catalog/product/view/id/1333052026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02095-1-siah1-primary-antibodies-wb-testing-1.jpgAnti-SIAH1 Antibody Picoband®Western blot analysis of SIAH1 using anti-SIAH1 antibody (A02095-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIAH1 antigen affinity purified polyclonal antibody (A02095-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SIAH1 at approximately 65 kDa. The expected band size for SIAH1 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02095-1-siah1-primary-antibodies-ihc-testing-1.jpgAnti-SIAH1 Antibody Picoband®IHC analysis of SIAH1 using anti-SIAH1 antibody (A02095-1). SIAH1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIAH1 Antibody (A02095-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02095-1-siah1-primary-antibodies-ihc-testing-2.jpgAnti-SIAH1 Antibody Picoband®IHC analysis of SIAH1 using anti-SIAH1 antibody (A02095-1). SIAH1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIAH1 Antibody (A02095-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02095-1-siah1-primary-antibodies-ihc-testing-3.jpgAnti-SIAH1 Antibody Picoband®IHC analysis of SIAH1 using anti-SIAH1 antibody (A02095-1). SIAH1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SIAH1 Antibody (A02095-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02095-1-siah1-primary-antibodies-if-testing-1.jpgAnti-SIAH1 Antibody Picoband®IF analysis of SIAH1 using anti-SIAH1 antibody (A02095-1). SIAH1 was detected in a paraffin-embedded section of SIHA tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SIAH1 Antibody (A02095-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02095-1-siah1-primary-antibodies-fcm-testing-1.jpgAnti-SIAH1 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-SIAH1 antibody (A02095-1). Overlay histogram showing HepG2 cells stained with A02095-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SIAH1 Antibody (A02095-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333062026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05151-2-sirpa-primary-antibodies-wb-testing-1.jpgAnti-SIRP Alpha/SIRPA Antibody Picoband®Western blot analysis of SIRPA using anti-SIRPA antibody (A05151-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRPA antigen affinity purified polyclonal antibody (A05151-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SIRPA at approximately 55 kDa. The expected band size for SIRPA is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05151-2-sirpa-primary-antibodies-if-testing-1.jpgAnti-SIRP Alpha/SIRPA Antibody Picoband®IF analysis of SIRPA using anti-SIRPA antibody (A05151-2). SIRPA was detected in a paraffin-embedded section of U2OS tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SIRPA Antibody (A05151-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05151-2-sirpa-primary-antibodies-fcm-testing-1.jpgAnti-SIRP Alpha/SIRPA Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-SIRPA antibody (A05151-2). Overlay histogram showing THP-1 cells stained with A05151-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SIRPA Antibody (A05151-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333072026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13531-1-dcaf4-primary-antibodies-wb-testing-1.jpgAnti-WDR21A/DCAF4 Antibody Picoband®Western blot analysis of DCAF4 using anti-DCAF4 antibody (A13531-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCAF4 antigen affinity purified polyclonal antibody (A13531-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCAF4 at approximately 56 kDa. The expected band size for DCAF4 is at 56 kDa. https://www.bosterbio.com/catalog/product/view/id/1333082026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14045-1-dcaf11-primary-antibodies-wb-testing-1.jpgAnti-DCAF11 Antibody Picoband®Western blot analysis of DCAF11 using anti-DCAF11 antibody (A14045-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCAF11 antigen affinity purified polyclonal antibody (A14045-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCAF11 at approximately 62 kDa. The expected band size for DCAF11 is at 62 kDa. https://www.bosterbio.com/catalog/product/view/id/1333092026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11802-1-dcaf12-primary-antibodies-wb-testing-1.jpgAnti-DCAF12 Antibody Picoband®Western blot analysis of DCAF12 using anti-DCAF12 antibody (A11802-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat ovary tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCAF12 antigen affinity purified polyclonal antibody (A11802-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCAF12 at approximately 50 kDa. The expected band size for DCAF12 is at 51 kDa. https://www.bosterbio.com/catalog/product/view/id/1333102026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15723-1-dcaf16-primary-antibodies-wb-testing-1.jpgAnti-DCAF16 Antibody Picoband®Western blot analysis of DCAF16 using anti-DCAF16 antibody (A15723-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human REH whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCAF16 antigen affinity purified polyclonal antibody (A15723-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCAF16 at approximately 22 kDa. The expected band size for DCAF16 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15723-1-dcaf16-primary-antibodies-ihc-testing-1.jpgAnti-DCAF16 Antibody Picoband®IHC analysis of DCAF16 using anti-DCAF16 antibody (A15723-1). DCAF16 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DCAF16 Antibody (A15723-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15723-1-dcaf16-primary-antibodies-if-testing-1.jpgAnti-DCAF16 Antibody Picoband®IF analysis of DCAF16 using anti-DCAF16 antibody (A15723-1). DCAF16 was detected in a paraffin-embedded section of Hela tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DCAF16 Antibody (A15723-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1333112026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06328-1-dcbld1-primary-antibodies-wb-testing-1.jpgAnti-DCBLD1 Antibody Picoband®Western blot analysis of DCBLD1 using anti-DCBLD1 antibody (A06328-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCBLD1 antigen affinity purified polyclonal antibody (A06328-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCBLD1 at approximately 100 kDa. The expected band size for DCBLD1 is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06328-1-dcbld1-primary-antibodies-fcm-testing-1.jpgAnti-DCBLD1 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-DCBLD1 antibody (A06328-1). Overlay histogram showing SH-SY5Y cells stained with A06328-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DCBLD1 Antibody (A06328-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333122026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06902-1-dctd-primary-antibodies-wb-testing-1.jpgAnti-DCTD Antibody Picoband®Western blot analysis of DCTD using anti-DCTD antibody (A06902-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCTD antigen affinity purified polyclonal antibody (A06902-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCTD at approximately 18 kDa. The expected band size for DCTD is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06902-1-dctd-primary-antibodies-fcm-testing-1.jpgAnti-DCTD Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-DCTD antibody (A06902-1). Overlay histogram showing A549 cells stained with A06902-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DCTD Antibody (A06902-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333132026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15471-2-slc16a13-primary-antibodies-ihc-testing-1.jpgAnti-SLC16A13 AntibodyIHC analysis of SLC16A13 using anti-SLC16A13 antibody (A15471-2). SLC16A13 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC16A13 Antibody (A15471-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333142026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06860-1-slc22a16-primary-antibodies-ihc-testing-1.jpgAnti-SLC22A16 AntibodyIHC analysis of SLC22A16 using anti-SLC22A16 antibody (A06860-1). SLC22A16 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC22A16 Antibody (A06860-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333152026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17175-1-slc22a24-primary-antibodies-wb-testing-1.jpgAnti-SLC22A24 Antibody Picoband®Western blot analysis of SLC22A24 using anti-SLC22A24 antibody (A17175-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC22A24 antigen affinity purified polyclonal antibody (A17175-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC22A24 at approximately 62 kDa. The expected band size for SLC22A24 is at 62 kDa. https://www.bosterbio.com/catalog/product/view/id/1333162026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17006-1-slc22a25-primary-antibodies-ihc-testing-1.jpgAnti-SLC22A25 AntibodyIHC analysis of SLC22A25 using anti-SLC22A25 antibody (A17006-1). SLC22A25 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC22A25 Antibody (A17006-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17006-1-slc22a25-primary-antibodies-ihc-testing-2.jpgAnti-SLC22A25 AntibodyIHC analysis of SLC22A25 using anti-SLC22A25 antibody (A17006-1). SLC22A25 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC22A25 Antibody (A17006-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333172026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08076-1-slc25a3-primary-antibodies-wb-testing-1.jpgAnti-SLC25A3 Antibody Picoband®Western blot analysis of SLC25A3 using anti-SLC25A3 antibody (A08076-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A3 antigen affinity purified polyclonal antibody (A08076-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC25A3 at approximately 40 kDa. The expected band size for SLC25A3 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08076-1-slc25a3-primary-antibodies-ihc-testing-1.jpgAnti-SLC25A3 Antibody Picoband®IHC analysis of SLC25A3 using anti-SLC25A3 antibody (A08076-1). SLC25A3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A3 Antibody (A08076-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08076-1-slc25a3-primary-antibodies-ihc-testing-2.jpgAnti-SLC25A3 Antibody Picoband®IHC analysis of SLC25A3 using anti-SLC25A3 antibody (A08076-1). SLC25A3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A3 Antibody (A08076-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08076-1-slc25a3-primary-antibodies-ihc-testing-3.jpgAnti-SLC25A3 Antibody Picoband®IHC analysis of SLC25A3 using anti-SLC25A3 antibody (A08076-1). SLC25A3 was detected in a paraffin-embedded section of human lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A3 Antibody (A08076-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333182026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09597-2-slc25a31-primary-antibodies-ihc-testing-1.jpgAnti-ANT4/SLC25A31 AntibodyIHC analysis of SLC25A31 using anti-SLC25A31 antibody (A09597-2). SLC25A31 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A31 Antibody (A09597-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09597-2-slc25a31-primary-antibodies-ihc-testing-2.jpgAnti-ANT4/SLC25A31 AntibodyIHC analysis of SLC25A31 using anti-SLC25A31 antibody (A09597-2). SLC25A31 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A31 Antibody (A09597-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333192026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08949-1-spata2-primary-antibodies-wb-testing-1.jpgAnti-SPATA2 Antibody Picoband®Western blot analysis of SPATA2 using anti-SPATA2 antibody (A08949-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA2 antigen affinity purified polyclonal antibody (A08949-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPATA2 at approximately 58 kDa. The expected band size for SPATA2 is at 58 kDa. https://www.bosterbio.com/catalog/product/view/id/1333202026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12239-1-srpk3-primary-antibodies-wb-testing-1.jpgAnti-SRPK3 Antibody Picoband®Western blot analysis of SRPK3 using anti-SRPK3 antibody (A12239-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRPK3 antigen affinity purified polyclonal antibody (A12239-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SRPK3 at approximately 70 kDa. The expected band size for SRPK3 is at 62 kDa. https://www.bosterbio.com/catalog/product/view/id/1333212026-03-17T05:17:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09134-1-stk40-primary-antibodies-wb-testing-1.jpgAnti-STK40 Antibody Picoband®Western blot analysis of STK40 using anti-STK40 antibody (A09134-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STK40 antigen affinity purified polyclonal antibody (A09134-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STK40 at approximately 49 kDa. The expected band size for STK40 is at 49 kDa. https://www.bosterbio.com/catalog/product/view/id/1333222026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12992-2-polr3c-primary-antibodies-wb-testing-1.jpgAnti-POLR3C Antibody Picoband®Western blot analysis of POLR3C using anti-POLR3C antibody (A12992-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR3C antigen affinity purified polyclonal antibody (A12992-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POLR3C at approximately 61 kDa. The expected band size for POLR3C is at 61 kDa. https://www.bosterbio.com/catalog/product/view/id/1333232026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06996-3-pphln1-primary-antibodies-wb-testing-1.jpgAnti-Periphilin 1/PPHLN1 Antibody Picoband®Western blot analysis of PPHLN1 using anti-PPHLN1 antibody (A06996-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human REH whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPHLN1 antigen affinity purified polyclonal antibody (A06996-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPHLN1 at approximately 60 kDa. The expected band size for PPHLN1 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06996-3-pphln1-primary-antibodies-if-testing-1.jpgAnti-Periphilin 1/PPHLN1 Antibody Picoband®IF analysis of PPHLN1 using anti-PPHLN1 antibody (A06996-3) and anti-Alpha Tubulin antibody (M03989-3). PPHLN1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPHLN1 Antibody (A06996-3) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1333242026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08091-2-grin2d-primary-antibodies-wb-testing-1.jpgAnti-GRIN2D Antibody Picoband®Western blot analysis of GRIN2D using anti-GRIN2D antibody (A08091-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIN2D antigen affinity purified polyclonal antibody (A08091-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRIN2D at approximately 165 kDa. The expected band size for GRIN2D is at 144 kDa. https://www.bosterbio.com/catalog/product/view/id/1333252026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10121-1-prr11-primary-antibodies-wb-testing-1.jpgAnti-PRR11 Antibody Picoband®Western blot analysis of PRR11 using anti-PRR11 antibody (A10121-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRR11 antigen affinity purified polyclonal antibody (A10121-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRR11 at approximately 38 kDa. The expected band size for PRR11 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10121-1-prr11-primary-antibodies-ihc-testing-1.jpgAnti-PRR11 Antibody Picoband®IHC analysis of PRR11 using anti-PRR11 antibody (A10121-1). PRR11 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRR11 Antibody (A10121-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10121-1-prr11-primary-antibodies-ihc-testing-2.jpgAnti-PRR11 Antibody Picoband®IHC analysis of PRR11 using anti-PRR11 antibody (A10121-1). PRR11 was detected in a paraffin-embedded section of human lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRR11 Antibody (A10121-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10121-1-prr11-primary-antibodies-ihc-testing-3.jpgAnti-PRR11 Antibody Picoband®IHC analysis of PRR11 using anti-PRR11 antibody (A10121-1). PRR11 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRR11 Antibody (A10121-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10121-1-prr11-primary-antibodies-if-testing-1.jpgAnti-PRR11 Antibody Picoband®IF analysis of PRR11 using anti-PRR11 antibody (A10121-1). PRR11 was detected in a paraffin-embedded section of U2OS tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PRR11 Antibody (A10121-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10121-1-prr11-primary-antibodies-fcm-testing-1.jpgAnti-PRR11 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-PRR11 antibody (A10121-1). Overlay histogram showing MCF-7 cells stained with A10121-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRR11 Antibody (A10121-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333262026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05110-2-ptafr-primary-antibodies-wb-testing-1.jpgAnti-PTAFR Antibody Picoband®Western blot analysis of PTAFR using anti-PTAFR antibody (A05110-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTAFR antigen affinity purified polyclonal antibody (A05110-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTAFR at approximately 39 kDa. The expected band size for PTAFR is at 39 kDa. https://www.bosterbio.com/catalog/product/view/id/1333272026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04983-1-qdpr-primary-antibodies-wb-testing-1.jpgAnti-QDPR Antibody Picoband®Western blot analysis of QDPR using anti-QDPR antibody (A04983-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QDPR antigen affinity purified polyclonal antibody (A04983-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for QDPR at approximately 26 kDa. The expected band size for QDPR is at 26 kDa. https://www.bosterbio.com/catalog/product/view/id/1333282026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05683-3-rab21-primary-antibodies-wb-testing-1.jpgAnti-RAB21 Antibody Picoband®Western blot analysis of RAB21 using anti-RAB21 antibody (A05683-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB21 antigen affinity purified polyclonal antibody (A05683-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB21 at approximately 24 kDa. The expected band size for RAB21 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05683-3-rab21-primary-antibodies-fcm-testing-1.jpgAnti-RAB21 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-RAB21 antibody (A05683-3). Overlay histogram showing MCF-7 cells stained with A05683-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB21 Antibody (A05683-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333292026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14182-2-syde2-primary-antibodies-wb-testing-1.jpgAnti-SYDE2 Antibody Picoband®Western blot analysis of SYDE2 using anti-SYDE2 antibody (A14182-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYDE2 antigen affinity purified polyclonal antibody (A14182-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SYDE2 at approximately 110 kDa. The expected band size for SYDE2 is at 133 kDa. https://www.bosterbio.com/catalog/product/view/id/1333302026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05025-1-cxxc1-primary-antibodies-wb-testing-1.jpgAnti-CXXC1 Antibody Picoband®Western blot analysis of CXXC1 using anti-CXXC1 antibody (A05025-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXXC1 antigen affinity purified polyclonal antibody (A05025-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CXXC1 at approximately 78 kDa. The expected band size for CXXC1 is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05025-1-cxxc1-primary-antibodies-if-testing-1.jpgAnti-CXXC1 Antibody Picoband®IF analysis of CXXC1 using anti-CXXC1 antibody (A05025-1) and anti-Alpha Tubulin antibody (M03989-3). CXXC1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CXXC1 Antibody (A05025-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05025-1-cxxc1-primary-antibodies-fcm-testing-1.jpgAnti-CXXC1 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-CXXC1 antibody (A05025-1). Overlay histogram showing SH-SY5Y cells stained with A05025-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CXXC1 Antibody (A05025-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tau-antibody-monoclonal-m00097-13-boster.html2026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vinculin-antibody-monoclonal-m01207-4-boster.html2026-03-16T05:11:21+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vimentin-antibody-monoclonal-m00235-7-boster.html2026-03-17T05:17:43+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpha-actinin-antibody-monoclonal-m03673-2-boster.html2026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1333352026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03559-2-rbm5-primary-antibodies-wb-testing-1.jpgAnti-RBM5 Antibody Picoband®Western blot analysis of RBM5 using anti-RBM5 antibody (A03559-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBM5 antigen affinity purified polyclonal antibody (A03559-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBM5 at approximately 115 kDa. The expected band size for RBM5 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03559-2-rbm5-primary-antibodies-fcm-testing-1.jpgAnti-RBM5 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-RBM5 antibody (A03559-2). Overlay histogram showing HEL cells stained with A03559-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM5 Antibody (A03559-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333362026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13938-slc25a47-primary-antibodies-ihc-testing-1.jpgAnti-SLC25A47 AntibodyIHC analysis of SLC25A47 using anti-SLC25A47 antibody (A13938). SLC25A47 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC25A47 Antibody (A13938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333372026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11831-2-slc9a7-primary-antibodies-ihc-testing-1.jpgAnti-SLC9A7 AntibodyIHC analysis of SLC9A7 using anti-SLC9A7 antibody (A11831-2). SLC9A7 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A7 Antibody (A11831-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333382026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08579-1-slc13a3-primary-antibodies-wb-testing-1.jpgAnti-SLC13A3 Antibody Picoband®Western blot analysis of SLC13A3 using anti-SLC13A3 antibody (A08579-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC13A3 antigen affinity purified polyclonal antibody (A08579-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC13A3 at approximately 67 kDa. The expected band size for SLC13A3 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08579-1-slc13a3-primary-antibodies-if-testing-1.jpgAnti-SLC13A3 Antibody Picoband®IF analysis of SLC13A3 using anti-SLC13A3 antibody (A08579-1). SLC13A3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLC13A3 Antibody (A08579-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1333392026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06094-slc27a1-primary-antibodies-wb-testing-1.jpgAnti-SLC27A1 Antibody Picoband®Western blot analysis of SLC27A1 using anti-SLC27A1 antibody (A06094). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC27A1 antigen affinity purified polyclonal antibody (A06094) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC27A1 at approximately 71 kDa. The expected band size for SLC27A1 is at 71 kDa. https://www.bosterbio.com/catalog/product/view/id/1333402026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04749-1-slc29a3-primary-antibodies-ihc-testing-1.jpgAnti-SLC29A3 AntibodyIHC analysis of SLC29A3 using anti-SLC29A3 antibody (A04749-1). SLC29A3 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC29A3 Antibody (A04749-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04749-1-slc29a3-primary-antibodies-ihc-testing-2.jpgAnti-SLC29A3 AntibodyIHC analysis of SLC29A3 using anti-SLC29A3 antibody (A04749-1). SLC29A3 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC29A3 Antibody (A04749-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333412026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05201-1-slc30a1-primary-antibodies-wb-testing-1.jpgAnti-SLC30A1 Antibody Picoband®Western blot analysis of SLC30A1 using anti-SLC30A1 antibody (A05201-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC30A1 antigen affinity purified polyclonal antibody (A05201-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC30A1 at approximately 50 kDa. The expected band size for SLC30A1 is at 55 kDa. https://www.bosterbio.com/catalog/product/view/id/1333422026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09062-1-lrrc26-primary-antibodies-wb-testing-1.jpgAnti-LRRC26 Antibody Picoband®Western blot analysis of LRRC26 using anti-LRRC26 antibody (A09062-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC26 antigen affinity purified polyclonal antibody (A09062-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LRRC26 at approximately 35 kDa. The expected band size for LRRC26 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09062-1-lrrc26-primary-antibodies-ihc-testing-1.jpgAnti-LRRC26 Antibody Picoband®IHC analysis of LRRC26 using anti-LRRC26 antibody (A09062-1). LRRC26 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LRRC26 Antibody (A09062-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09062-1-lrrc26-primary-antibodies-ihc-testing-2.jpgAnti-LRRC26 Antibody Picoband®IHC analysis of LRRC26 using anti-LRRC26 antibody (A09062-1). LRRC26 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LRRC26 Antibody (A09062-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09062-1-lrrc26-primary-antibodies-fcm-testing-1.jpgAnti-LRRC26 Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-LRRC26 antibody (A09062-1). Overlay histogram showing Caco-2 cells stained with A09062-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LRRC26 Antibody (A09062-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333432026-03-17T05:17:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11961-prr3-primary-antibodies-wb-testing-1.jpgAnti-PRR3 Antibody Picoband®Western blot analysis of PRR3 using anti-PRR3 antibody (A11961). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRR3 antigen affinity purified polyclonal antibody (A11961) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRR3 at approximately 28 kDa. The expected band size for PRR3 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11961-prr3-primary-antibodies-wb-testing-2.jpgAnti-PRR3 Antibody Picoband®Western blot analysis of PRR3 using anti-PRR3 antibody (A11961). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRR3 antigen affinity purified polyclonal antibody (A11961) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRR3 at approximately 28 kDa. The expected band size for PRR3 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11961-prr3-primary-antibodies-if-testing-1.jpgAnti-PRR3 Antibody Picoband®IF analysis of PRR3 using anti-PRR3 antibody (A11961) and anti-Alpha Tubulin antibody (M03989-3). PRR3 was detected in an immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRR3 Antibody (A11961) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11961-prr3-primary-antibodies-fcm-testing-1.jpgAnti-PRR3 Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-PRR3 antibody (A11961). Overlay histogram showing Caco-2 cells stained with A11961 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRR3 Antibody (A11961, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333442026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12856-2-ptgdr2-primary-antibodies-wb-testing-1.jpgAnti-GPR44/PTGDR2 Antibody Picoband®Western blot analysis of PTGDR2 using anti-PTGDR2 antibody (A12856-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGDR2 antigen affinity purified polyclonal antibody (A12856-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTGDR2 at approximately 45,55 kDa. The expected band size for PTGDR2 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12856-2-ptgdr2-primary-antibodies-ihc-testing-1.jpgAnti-GPR44/PTGDR2 Antibody Picoband®IHC analysis of PTGDR2 using anti-PTGDR2 antibody (A12856-2). PTGDR2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTGDR2 Antibody (A12856-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12856-2-ptgdr2-primary-antibodies-fcm-testing-1.jpgAnti-GPR44/PTGDR2 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-PTGDR2 antibody (A12856-2). Overlay histogram showing 293T cells stained with A12856-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGDR2 Antibody (A12856-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333452026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06517-1-qpct-primary-antibodies-wb-testing-1.jpgAnti-QPCT Antibody Picoband®Western blot analysis of QPCT using anti-QPCT antibody (A06517-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QPCT antigen affinity purified polyclonal antibody (A06517-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for QPCT at approximately 35 kDa. The expected band size for QPCT is at 41 kDa. https://www.bosterbio.com/catalog/product/view/id/1333462026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06566-1-sla2-primary-antibodies-wb-testing-1.jpgAnti-SLA2 Antibody Picoband®Western blot analysis of SLA2 using anti-SLA2 antibody (A06566-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLA2 antigen affinity purified polyclonal antibody (A06566-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLA2 at approximately 28 kDa. The expected band size for SLA2 is at 28 kDa. https://www.bosterbio.com/catalog/product/view/id/1333472026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04083-2-slamf7-primary-antibodies-wb-testing-1.jpgAnti-CD319/SLAMF7 Antibody Picoband®Western blot analysis of SLAMF7 using anti-SLAMF7 antibody (A04083-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLAMF7 antigen affinity purified polyclonal antibody (A04083-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLAMF7 at approximately 70 kDa. The expected band size for SLAMF7 is at 37 kDa. https://www.bosterbio.com/catalog/product/view/id/1333482026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03633-2-sarm1-primary-antibodies-wb-testing-1.jpgAnti-SARM1 Antibody Picoband®Western blot analysis of SARM1 using anti-SARM1 antibody (A03633-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SARM1 antigen affinity purified polyclonal antibody (A03633-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SARM1 at approximately 75 kDa. The expected band size for SARM1 is at 79 kDa. https://www.bosterbio.com/catalog/product/view/id/1333492026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04851-1-sema3f-primary-antibodies-wb-testing-1.jpgAnti-SEMA3F Antibody Picoband®Western blot analysis of SEMA3F using anti-SEMA3F antibody (A04851-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3F antigen affinity purified polyclonal antibody (A04851-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SEMA3F at approximately 100 kDa. The expected band size for SEMA3F is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04851-1-sema3f-primary-antibodies-ihc-testing-1.jpgAnti-SEMA3F Antibody Picoband®IHC analysis of SEMA3F using anti-SEMA3F antibody (A04851-1). SEMA3F was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEMA3F Antibody (A04851-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04851-1-sema3f-primary-antibodies-ihc-testing-2.jpgAnti-SEMA3F Antibody Picoband®IHC analysis of SEMA3F using anti-SEMA3F antibody (A04851-1). SEMA3F was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SEMA3F Antibody (A04851-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04851-1-sema3f-primary-antibodies-fcm-testing-1.jpgAnti-SEMA3F Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-SEMA3F antibody (A04851-1). Overlay histogram showing RT4 cells stained with A04851-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SEMA3F Antibody (A04851-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333502026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02329-3-senp2-primary-antibodies-wb-testing-1.jpgAnti-SENP2 Antibody Picoband®Western blot analysis of SENP2 using anti-SENP2 antibody (A02329-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP2 antigen affinity purified polyclonal antibody (A02329-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SENP2 at approximately 68 kDa. The expected band size for SENP2 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02329-3-senp2-primary-antibodies-fcm-testing-1.jpgAnti-SENP2 Antibody Picoband®Flow Cytometry analysis of HeLa cells using anti-SENP2 antibody (A02329-3). Overlay histogram showing HeLa cells stained with A02329-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SENP2 Antibody (A02329-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333512026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08121-3-senp7-primary-antibodies-wb-testing-1.jpgAnti-SENP7 Antibody Picoband®Western blot analysis of SENP7 using anti-SENP7 antibody (A08121-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP7 antigen affinity purified polyclonal antibody (A08121-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SENP7 at approximately 120 kDa. The expected band size for SENP7 is at 120 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08121-3-senp7-primary-antibodies-fcm-testing-1.jpgAnti-SENP7 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-SENP7 antibody (A08121-3). Overlay histogram showing SH-SY5Y cells stained with A08121-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SENP7 Antibody (A08121-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333522026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11524-1-sfxn1-primary-antibodies-wb-testing-1.jpgAnti-SFXN1 Antibody Picoband®Western blot analysis of SFXN1 using anti-SFXN1 antibody (A11524-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFXN1 antigen affinity purified polyclonal antibody (A11524-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SFXN1 at approximately 35 kDa. The expected band size for SFXN1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11524-1-sfxn1-primary-antibodies-ihc-testing-1.jpgAnti-SFXN1 Antibody Picoband®IHC analysis of SFXN1 using anti-SFXN1 antibody (A11524-1). SFXN1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN1 Antibody (A11524-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11524-1-sfxn1-primary-antibodies-ihc-testing-2.jpgAnti-SFXN1 Antibody Picoband®IHC analysis of SFXN1 using anti-SFXN1 antibody (A11524-1). SFXN1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SFXN1 Antibody (A11524-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11524-1-sfxn1-primary-antibodies-if-testing-1.jpgAnti-SFXN1 Antibody Picoband®IF analysis of SFXN1 using anti-SFXN1 antibody (A11524-1). SFXN1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SFXN1 Antibody (A11524-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11524-1-sfxn1-primary-antibodies-ip-testing-1.jpgAnti-SFXN1 Antibody Picoband®Immunoprecipitating SFXN1 in HepG2 whole cell lysate. Western blot analysis of SFXN1 using anti-SFXN1 antibody (A11524-1). Lane 1: HepG2 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-SFXN1 antibody in HepG2 whole cell lysate, Lane 3: anti-SFXN1 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SFXN1 antigen affinity purified polyclonal antibody (A11524-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SFXN1 at approximately 35 kDa. The expected band size for SFXN1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11524-1-sfxn1-primary-antibodies-fcm-testing-1.jpgAnti-SFXN1 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-SFXN1 antibody (A11524-1). Overlay histogram showing 293T cells stained with A11524-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFXN1 Antibody (A11524-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333532026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06519-2-dars-primary-antibodies-wb-testing-1.jpgAnti-DARS1 Antibody Picoband®Western blot analysis of DARS using anti-DARS antibody (A06519-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DARS antigen affinity purified polyclonal antibody (A06519-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DARS at approximately 50 kDa. The expected band size for DARS is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06519-2-dars-primary-antibodies-fcm-testing-1.jpgAnti-DARS1 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-DARS antibody (A06519-2). Overlay histogram showing K562 cells stained with A06519-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DARS Antibody (A06519-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333542026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14217-daw1-primary-antibodies-wb-testing-1.jpgAnti-DAW1 Antibody Picoband®Western blot analysis of DAW1 using anti-DAW1 antibody (A14217). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAW1 antigen affinity purified polyclonal antibody (A14217) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DAW1 at approximately 41 kDa. The expected band size for DAW1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14217-daw1-primary-antibodies-fcm-testing-1.jpgAnti-DAW1 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-DAW1 antibody (A14217). Overlay histogram showing A549 cells stained with A14217 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DAW1 Antibody (A14217, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333552026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10977-1-ddx18-primary-antibodies-wb-testing-1.jpgAnti-DDX18 Antibody Picoband®Western blot analysis of DDX18 using anti-DDX18 antibody (A10977-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX18 antigen affinity purified polyclonal antibody (A10977-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX18 at approximately 80 kDa. The expected band size for DDX18 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10977-1-ddx18-primary-antibodies-if-testing-1.jpgAnti-DDX18 Antibody Picoband®IF analysis of DDX18 using anti-DDX18 antibody (A10977-1) and anti-Alpha Tubulin antibody (M03989-3). DDX18 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDX18 Antibody (A10977-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10977-1-ddx18-primary-antibodies-ip-testing-1.jpgAnti-DDX18 Antibody Picoband®Immunoprecipitating DDX18 in HepG2 whole cell lysate. Western blot analysis of DDX18 using anti-DDX18 antibody (A10977-1). Lane 1: HepG2 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DDX18 antibody in HepG2 whole cell lysate, Lane 3: anti-DDX18 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DDX18 antigen affinity purified polyclonal antibody (A10977-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DDX18 at approximately 80 kDa. The expected band size for DDX18 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10977-1-ddx18-primary-antibodies-fcm-testing-1.jpgAnti-DDX18 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-DDX18 antibody (A10977-1). Overlay histogram showing SH-SY5Y cells stained with A10977-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX18 Antibody (A10977-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333562026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11446-1-ddx19a-b-primary-antibodies-wb-testing-1.jpgAnti-DDX19A/B Antibody Picoband®Western blot analysis of DDX19A/B using anti-DDX19A/B antibody (A11446-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX19A/B antigen affinity purified polyclonal antibody (A11446-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX19A/B at approximately 54 kDa. The expected band size for DDX19A/B is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11446-1-ddx19a-b-primary-antibodies-fcm-testing-1.jpgAnti-DDX19A/B Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-DDX19A/B antibody (A11446-1). Overlay histogram showing U251 cells stained with A11446-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX19A/B Antibody (A11446-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11446-1-ddx19a-b-primary-antibodies-ip-testing-1.jpgAnti-DDX19A/B Antibody Picoband®Immunoprecipitating DDX19A,B in U251 whole cell lysate. Western blot analysis of DDX19A,B using anti-DDX19A,B antibody (A11446-1). Lane 1: U251 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DDX19A,B antibody in U251 whole cell lysate, Lane 3: anti-DDX19A,B antibody (2μg) + U251 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DDX19A,B antigen affinity purified polyclonal antibody (A11446-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DDX19A,B at approximately 54 kDa. The expected band size for DDX19A,B is at 54 kDa. https://www.bosterbio.com/catalog/product/view/id/1333572026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09031-1-spata13-primary-antibodies-wb-testing-1.jpgAnti-SPATA13 Antibody Picoband®Western blot analysis of SPATA13 using anti-SPATA13 antibody (A09031-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA13 antigen affinity purified polyclonal antibody (A09031-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPATA13 at approximately 75 kDa. The expected band size for SPATA13 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09031-1-spata13-primary-antibodies-fcm-testing-1.jpgAnti-SPATA13 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-SPATA13 antibody (A09031-1). Overlay histogram showing RT4 cells stained with A09031-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPATA13 Antibody (A09031-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333582026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05007-1-rasgrp2-primary-antibodies-wb-testing-1.jpgAnti-RASGRP2 Antibody Picoband®Western blot analysis of RASGRP2 using anti-RASGRP2 antibody (A05007-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASGRP2 antigen affinity purified polyclonal antibody (A05007-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RASGRP2 at approximately 69 kDa. The expected band size for RASGRP2 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05007-1-rasgrp2-primary-antibodies-ihc-testing-1.jpgAnti-RASGRP2 Antibody Picoband®IHC analysis of RASGRP2 using anti-RASGRP2 antibody (A05007-1). RASGRP2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RASGRP2 Antibody (A05007-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05007-1-rasgrp2-primary-antibodies-fcm-testing-1.jpgAnti-RASGRP2 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-RASGRP2 antibody (A05007-1). Overlay histogram showing HEL cells stained with A05007-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-RASGRP2 Antibody (A05007-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333592026-03-17T05:17:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10439-1-ddx27-primary-antibodies-wb-testing-1.jpgAnti-DDX27 Antibody Picoband®Western blot analysis of DDX27 using anti-DDX27 antibody (A10439-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX27 antigen affinity purified polyclonal antibody (A10439-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX27 at approximately 87 kDa. The expected band size for DDX27 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10439-1-ddx27-primary-antibodies-if-testing-1.jpgAnti-DDX27 Antibody Picoband®IF analysis of DDX27 using anti-DDX27 antibody (A10439-1) and anti-Alpha Tubulin antibody (M03989-3). DDX27 was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDX27 Antibody (A10439-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10439-1-ddx27-primary-antibodies-ip-testing-1.jpgAnti-DDX27 Antibody Picoband®Immunoprecipitating DDX27 in U251 whole cell lysate. Western blot analysis of DDX27 using anti-DDX27 antibody (A10439-1). Lane 1: U251 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DDX27 antibody in U251 whole cell lysate, Lane 3: anti-DDX27 antibody (2μg) + U251 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DDX27 antigen affinity purified polyclonal antibody (A10439-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DDX27 at approximately 87 kDa. The expected band size for DDX27 is at 87 kDa. https://www.bosterbio.com/catalog/product/view/id/1333602026-03-17T05:17:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12493-1-ddx31-primary-antibodies-wb-testing-1.jpgAnti-DDX31 Antibody Picoband®Western blot analysis of DDX31 using anti-DDX31 antibody (A12493-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX31 antigen affinity purified polyclonal antibody (A12493-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX31 at approximately 110 kDa. The expected band size for DDX31 is at 94 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12493-1-ddx31-primary-antibodies-ihc-testing-1.jpgAnti-DDX31 Antibody Picoband®IHC analysis of DDX31 using anti-DDX31 antibody (A12493-1). DDX31 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX31 Antibody (A12493-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12493-1-ddx31-primary-antibodies-if-testing-1.jpgAnti-DDX31 Antibody Picoband®IF analysis of DDX31 using anti-DDX31 antibody (A12493-1) and anti-Alpha Tubulin antibody (M03989-3). DDX31 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDX31 Antibody (A12493-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1333612026-03-17T05:17:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12850-3-ddx55-primary-antibodies-wb-testing-1.jpgAnti-DDX55 Antibody Picoband®Western blot analysis of DDX55 using anti-DDX55 antibody (A12850-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX55 antigen affinity purified polyclonal antibody (A12850-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX55 at approximately 69 kDa. The expected band size for DDX55 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12850-3-ddx55-primary-antibodies-ihc-testing-1.jpgAnti-DDX55 Antibody Picoband®IHC analysis of DDX55 using anti-DDX55 antibody (A12850-3). DDX55 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX55 Antibody (A12850-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12850-3-ddx55-primary-antibodies-ihc-testing-2.jpgAnti-DDX55 Antibody Picoband®IHC analysis of DDX55 using anti-DDX55 antibody (A12850-3). DDX55 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX55 Antibody (A12850-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12850-3-ddx55-primary-antibodies-ihc-testing-3.jpgAnti-DDX55 Antibody Picoband®IHC analysis of DDX55 using anti-DDX55 antibody (A12850-3). DDX55 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX55 Antibody (A12850-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12850-3-ddx55-primary-antibodies-ihc-testing-4.jpgAnti-DDX55 Antibody Picoband®IHC analysis of DDX55 using anti-DDX55 antibody (A12850-3). DDX55 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX55 Antibody (A12850-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12850-3-ddx55-primary-antibodies-ip-testing-1.jpgAnti-DDX55 Antibody Picoband®Immunoprecipitating DDX55 in HEL whole cell lysate. Western blot analysis of DDX55 using anti-DDX55 antibody (A12850-3). Lane 1: HEL whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DDX55 antibody in HEL whole cell lysate, Lane 3: anti-DDX55 antibody (2μg) + HEL whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DDX55 antigen affinity purified polyclonal antibody (A12850-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DDX55 at approximately 69 kDa. The expected band size for DDX55 is at 69 kDa. https://www.bosterbio.com/catalog/product/view/id/1333622026-03-17T05:17:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10946-1-ddx56-primary-antibodies-wb-testing-1.jpgAnti-DDX56 Antibody Picoband®Western blot analysis of DDX56 using anti-DDX56 antibody (A10946-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX56 antigen affinity purified polyclonal antibody (A10946-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX56 at approximately 62 kDa. The expected band size for DDX56 is at 62 kDa. https://www.bosterbio.com/catalog/product/view/id/1333632026-03-17T05:17:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12681-1-ddx59-primary-antibodies-wb-testing-1.jpgAnti-DDX59 Antibody Picoband®Western blot analysis of DDX59 using anti-DDX59 antibody (A12681-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX59 antigen affinity purified polyclonal antibody (A12681-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX59 at approximately 75 kDa. The expected band size for DDX59 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12681-1-ddx59-primary-antibodies-if-testing-1.jpgAnti-DDX59 Antibody Picoband®IF analysis of DDX59 using anti-DDX59 antibody (A12681-1) and anti-Alpha Tubulin antibody (M03989-3). DDX59 was detected in an immunocytochemical section of SIHA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDX59 Antibody (A12681-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1333642026-03-17T05:17:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12572-2-dennd2d-primary-antibodies-wb-testing-1.jpgAnti-DENND2D Antibody Picoband®Western blot analysis of DENND2D using anti-DENND2D antibody (A12572-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DENND2D antigen affinity purified polyclonal antibody (A12572-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DENND2D at approximately 50 kDa. The expected band size for DENND2D is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12572-2-dennd2d-primary-antibodies-ihc-testing-1.jpgAnti-DENND2D Antibody Picoband®IHC analysis of DENND2D using anti-DENND2D antibody (A12572-2). DENND2D was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DENND2D Antibody (A12572-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12572-2-dennd2d-primary-antibodies-ihc-testing-2.jpgAnti-DENND2D Antibody Picoband®IHC analysis of DENND2D using anti-DENND2D antibody (A12572-2). DENND2D was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DENND2D Antibody (A12572-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12572-2-dennd2d-primary-antibodies-fcm-testing-1.jpgAnti-DENND2D Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-DENND2D antibody (A12572-2). Overlay histogram showing MCF-7 cells stained with A12572-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DENND2D Antibody (A12572-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333652026-03-17T05:17:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09430-1-dgcr2-primary-antibodies-wb-testing-1.jpgAnti-DGCR2 Antibody Picoband®Western blot analysis of DGCR2 using anti-DGCR2 antibody (A09430-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DGCR2 antigen affinity purified polyclonal antibody (A09430-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DGCR2 at approximately 61 kDa. The expected band size for DGCR2 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09430-1-dgcr2-primary-antibodies-fcm-testing-1.jpgAnti-DGCR2 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-DGCR2 antibody (A09430-1). Overlay histogram showing RT4 cells stained with A09430-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DGCR2 Antibody (A09430-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333662026-03-17T05:17:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06092-1-dguok-primary-antibodies-wb-testing-1.jpgAnti-DGUOK Antibody Picoband®Western blot analysis of DGUOK using anti-DGUOK antibody (A06092-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DGUOK antigen affinity purified polyclonal antibody (A06092-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DGUOK at approximately 28 kDa. The expected band size for DGUOK is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06092-1-dguok-primary-antibodies-fcm-testing-1.jpgAnti-DGUOK Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-DGUOK antibody (A06092-1). Overlay histogram showing A549 cells stained with A06092-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DGUOK Antibody (A06092-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333672026-03-17T05:17:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09237-dhx30-primary-antibodies-wb-testing-1.jpgAnti-DHX30 Antibody Picoband®Western blot analysis of DHX30 using anti-DHX30 antibody (A09237). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX30 antigen affinity purified polyclonal antibody (A09237) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHX30 at approximately 134 kDa. The expected band size for DHX30 is at 134 kDa. https://www.bosterbio.com/catalog/product/view/id/1333682026-03-17T05:17:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10163-1-dhx34-primary-antibodies-wb-testing-1.jpgAnti-DHX34 Antibody Picoband®Western blot analysis of DHX34 using anti-DHX34 antibody (A10163-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX34 antigen affinity purified polyclonal antibody (A10163-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHX34 at approximately 128 kDa. The expected band size for DHX34 is at 128 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10163-1-dhx34-primary-antibodies-ihc-testing-1.jpgAnti-DHX34 Antibody Picoband®IHC analysis of DHX34 using anti-DHX34 antibody (A10163-1). DHX34 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX34 Antibody (A10163-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10163-1-dhx34-primary-antibodies-ihc-testing-2.jpgAnti-DHX34 Antibody Picoband®IHC analysis of DHX34 using anti-DHX34 antibody (A10163-1). DHX34 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX34 Antibody (A10163-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10163-1-dhx34-primary-antibodies-ihc-testing-3.jpgAnti-DHX34 Antibody Picoband®IHC analysis of DHX34 using anti-DHX34 antibody (A10163-1). DHX34 was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX34 Antibody (A10163-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333692026-03-17T05:17:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03105-2-tdgf1-primary-antibodies-wb-testing-1.jpgAnti-CRIPTO Antibody Picoband®Western blot analysis of TDGF1 using anti-TDGF1 antibody (A03105-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDGF1 antigen affinity purified polyclonal antibody (A03105-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TDGF1 at approximately 25 kDa. The expected band size for TDGF1 is at 21 kDa. https://www.bosterbio.com/catalog/product/view/id/1333702026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01266-1-tes-primary-antibodies-wb-testing-1.jpgAnti-TES Antibody Picoband®Western blot analysis of TES using anti-TES antibody (A01266-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TES antigen affinity purified polyclonal antibody (A01266-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TES at approximately 40 kDa. The expected band size for TES is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01266-1-tes-primary-antibodies-fcm-testing-1.jpgAnti-TES Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-TES antibody (A01266-1). Overlay histogram showing SH-SY5Y cells stained with A01266-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TES Antibody (A01266-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333712026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14858-1-dhx37-primary-antibodies-wb-testing-1.jpgAnti-DHX37 Antibody Picoband®Western blot analysis of DHX37 using anti-DHX37 antibody (A14858-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX37 antigen affinity purified polyclonal antibody (A14858-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHX37 at approximately 150 kDa. The expected band size for DHX37 is at 130 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14858-1-dhx37-primary-antibodies-if-testing-1.jpgAnti-DHX37 Antibody Picoband®IF analysis of DHX37 using anti-DHX37 antibody (A14858-1) and anti-Alpha Tubulin antibody (M03989-3). DHX37 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DHX37 Antibody (A14858-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14858-1-dhx37-primary-antibodies-fcm-testing-1.jpgAnti-DHX37 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-DHX37 antibody (A14858-1). Overlay histogram showing 293T cells stained with A14858-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DHX37 Antibody (A14858-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333722026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14749-1-dhx40-primary-antibodies-wb-testing-1.jpgAnti-DHX40 Antibody Picoband®Western blot analysis of DHX40 using anti-DHX40 antibody (A14749-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX40 antigen affinity purified polyclonal antibody (A14749-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHX40 at approximately 89 kDa. The expected band size for DHX40 is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14749-1-dhx40-primary-antibodies-ihc-testing-1.jpgAnti-DHX40 Antibody Picoband®IHC analysis of DHX40 using anti-DHX40 antibody (A14749-1). DHX40 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX40 Antibody (A14749-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14749-1-dhx40-primary-antibodies-ihc-testing-2.jpgAnti-DHX40 Antibody Picoband®IHC analysis of DHX40 using anti-DHX40 antibody (A14749-1). DHX40 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHX40 Antibody (A14749-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14749-1-dhx40-primary-antibodies-if-testing-1.jpgAnti-DHX40 Antibody Picoband®IF analysis of DHX40 using anti-DHX40 antibody (A14749-1) and anti-Alpha Tubulin antibody (M03989-3). DHX40 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DHX40 Antibody (A14749-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1333732026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12162-1-dhx57-primary-antibodies-wb-testing-1.jpgAnti-DHX57 Antibody Picoband®Western blot analysis of DHX57 using anti-DHX57 antibody (A12162-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHX57 antigen affinity purified polyclonal antibody (A12162-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHX57 at approximately 156 kDa. The expected band size for DHX57 is at 156 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12162-1-dhx57-primary-antibodies-if-testing-1.jpgAnti-DHX57 Antibody Picoband®IF analysis of DHX57 using anti-DHX57 antibody (A12162-1). DHX57 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DHX57 Antibody (A12162-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12162-1-dhx57-primary-antibodies-fcm-testing-1.jpgAnti-DHX57 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-DHX57 antibody (A12162-1). Overlay histogram showing 293T cells stained with A12162-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DHX57 Antibody (A12162-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333742026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07738-1-dis3l2-primary-antibodies-wb-testing-1.jpgAnti-DIS3L2 Antibody Picoband®Western blot analysis of DIS3l2 using anti-DIS3l2 antibody (A07738-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIS3l2 antigen affinity purified polyclonal antibody (A07738-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DIS3l2 at approximately 99 kDa. The expected band size for DIS3l2 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07738-1-dis3l2-primary-antibodies-ihc-testing-1.jpgAnti-DIS3L2 Antibody Picoband®IHC analysis of DIS3l2 using anti-DIS3l2 antibody (A07738-1). DIS3l2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DIS3l2 Antibody (A07738-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07738-1-dis3l2-primary-antibodies-ip-testing-1.jpgAnti-DIS3L2 Antibody Picoband®Immunoprecipitating DIS3l2 in HepG2 whole cell lysate. Western blot analysis of DIS3l2 using anti-DIS3l2 antibody (A07738-1). Lane 1: HepG2 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DIS3l2 antibody in HepG2 whole cell lysate, Lane 3: anti-DIS3l2 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DIS3l2 antigen affinity purified polyclonal antibody (A07738-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DIS3l2 at approximately 99 kDa. The expected band size for DIS3l2 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07738-1-dis3l2-primary-antibodies-fcm-testing-1.jpgAnti-DIS3L2 Antibody Picoband®Flow Cytometry analysis of HepG2 cells using anti-DIS3l2 antibody (A07738-1). Overlay histogram showing HepG2 cells stained with A07738-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DIS3l2 Antibody (A07738-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333752026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04374-dlg5-primary-antibodies-wb-testing-1.jpgAnti-DLG5 Antibody Picoband®Western blot analysis of DLG5 using anti-DLG5 antibody (A04374). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLG5 antigen affinity purified polyclonal antibody (A04374) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DLG5 at approximately 75240 kDa. The expected band size for DLG5 is at 214 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04374-dlg5-primary-antibodies-ihc-testing-1.jpgAnti-DLG5 Antibody Picoband®IHC analysis of DLG5 using anti-DLG5 antibody (A04374). DLG5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DLG5 Antibody (A04374) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04374-dlg5-primary-antibodies-fcm-testing-1.jpgAnti-DLG5 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-DLG5 antibody (A04374). Overlay histogram showing K562 cells stained with A04374 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DLG5 Antibody (A04374, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333762026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11922-1-dlgap4-primary-antibodies-wb-testing-1.jpgAnti-DLGAP4 Antibody Picoband®Western blot analysis of DLGAP4 using anti-DLGAP4 antibody (A11922-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLGAP4 antigen affinity purified polyclonal antibody (A11922-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DLGAP4 at approximately 150 kDa. The expected band size for DLGAP4 is at 108 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11922-1-dlgap4-primary-antibodies-fcm-testing-1.jpgAnti-DLGAP4 Antibody Picoband®Flow Cytometry analysis of Caco-2 cells using anti-DLGAP4 antibody (A11922-1). Overlay histogram showing Caco-2 cells stained with A11922-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DLGAP4 Antibody (A11922-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333772026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09344-dnpep-primary-antibodies-wb-testing-1.jpgAnti-DNPEP Antibody Picoband®Western blot analysis of DNPEP using anti-DNPEP antibody (A09344). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates, Lane 3: mouse CT26.WT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNPEP antigen affinity purified polyclonal antibody (A09344) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNPEP at approximately 53 kDa. The expected band size for DNPEP is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09344-dnpep-primary-antibodies-ihc-testing-1.jpgAnti-DNPEP Antibody Picoband®IHC analysis of DNPEP using anti-DNPEP antibody (A09344). DNPEP was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNPEP Antibody (A09344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333782026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03836-3-dock2-primary-antibodies-wb-testing-1.jpgAnti-DOCK2 Antibody Picoband®Western blot analysis of DOCK2 using anti-DOCK2 antibody (A03836-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Ramos whole cell lysates, Lane 2: rat thymus tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOCK2 antigen affinity purified polyclonal antibody (A03836-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DOCK2 at approximately 200 kDa. The expected band size for DOCK2 is at 212 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03836-3-dock2-primary-antibodies-fcm-testing-1.jpgAnti-DOCK2 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-DOCK2 antibody (A03836-3). Overlay histogram showing Jurkat cells stained with A03836-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DOCK2 Antibody (A03836-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333792026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06349-dock6-primary-antibodies-wb-testing-1.jpgAnti-DOCK6 Antibody Picoband®Western blot analysis of DOCK6 using anti-DOCK6 antibody (A06349). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOCK6 antigen affinity purified polyclonal antibody (A06349) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DOCK6 at approximately 230 kDa. The expected band size for DOCK6 is at 230 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06349-dock6-primary-antibodies-ihc-testing-1.jpgAnti-DOCK6 Antibody Picoband®IHC analysis of DOCK6 using anti-DOCK6 antibody (A06349). DOCK6 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DOCK6 Antibody (A06349) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06349-dock6-primary-antibodies-fcm-testing-1.jpgAnti-DOCK6 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-DOCK6 antibody (A06349). Overlay histogram showing RT4 cells stained with A06349 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DOCK6 Antibody (A06349, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333802026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06829-2-ddx39a-b-primary-antibodies-wb-testing-1.jpgAnti-DDX39A/B Antibody Picoband®Western blot analysis of DDX39A/B using anti-DDX39A/B antibody (A06829-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX39A/B antigen affinity purified polyclonal antibody (A06829-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDX39A/B at approximately 50 kDa. The expected band size for DDX39A/B is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06829-2-ddx39a-b-primary-antibodies-ihc-testing-1.jpgAnti-DDX39A/B Antibody Picoband®IHC analysis of DDX39A/B using anti-DDX39A/B antibody (A06829-2). DDX39A/B was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX39A/B Antibody (A06829-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06829-2-ddx39a-b-primary-antibodies-ihc-testing-2.jpgAnti-DDX39A/B Antibody Picoband®IHC analysis of DDX39A/B using anti-DDX39A/B antibody (A06829-2). DDX39A/B was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX39A/B Antibody (A06829-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06829-2-ddx39a-b-primary-antibodies-ihc-testing-3.jpgAnti-DDX39A/B Antibody Picoband®IHC analysis of DDX39A/B using anti-DDX39A/B antibody (A06829-2). DDX39A/B was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDX39A/B Antibody (A06829-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06829-2-ddx39a-b-primary-antibodies-if-testing-1.jpgAnti-DDX39A/B Antibody Picoband®IF analysis of DDX39A/B using anti-DDX39A/B antibody (A06829-2) and anti-Alpha Tubulin antibody (M03989-3). DDX39A/B was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDX39A/B Antibody (A06829-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1333812026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08287-1-dph1-primary-antibodies-wb-testing-1.jpgAnti-DPH1 Antibody Picoband®Western blot analysis of DPH1 using anti-DPH1 antibody (A08287-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPH1 antigen affinity purified polyclonal antibody (A08287-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DPH1 at approximately 60 kDa. The expected band size for DPH1 is at 48 kDa. https://www.bosterbio.com/catalog/product/view/id/1333822026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11626-1-dph6-primary-antibodies-wb-testing-1.jpgAnti-DPH6 Antibody Picoband®Western blot analysis of DPH6 using anti-DPH6 antibody (A11626-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPH6 antigen affinity purified polyclonal antibody (A11626-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DPH6 at approximately 48 kDa. The expected band size for DPH6 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11626-1-dph6-primary-antibodies-if-testing-1.jpgAnti-DPH6 Antibody Picoband®IF analysis of DPH6 using anti-DPH6 antibody (A11626-1) and anti-Alpha Tubulin antibody (M03989-3). DPH6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DPH6 Antibody (A11626-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11626-1-dph6-primary-antibodies-fcm-testing-1.jpgAnti-DPH6 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-DPH6 antibody (A11626-1). Overlay histogram showing 293T cells stained with A11626-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DPH6 Antibody (A11626-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333832026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07788-1-dpp3-primary-antibodies-wb-testing-1.jpgAnti-DPP3 Antibody Picoband®Western blot analysis of DPP3 using anti-DPP3 antibody (A07788-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse thymus tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPP3 antigen affinity purified polyclonal antibody (A07788-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DPP3 at approximately 83 kDa. The expected band size for DPP3 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07788-1-dpp3-primary-antibodies-fcm-testing-1.jpgAnti-DPP3 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-DPP3 antibody (A07788-1). Overlay histogram showing Jurkat cells stained with A07788-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DPP3 Antibody (A07788-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333842026-03-17T05:17:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09935-drc1-primary-antibodies-wb-testing-1.jpgAnti-DRC1 Antibody Picoband®Western blot analysis of DRC1 using anti-DRC1 antibody (A09935). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRC1 antigen affinity purified polyclonal antibody (A09935) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DRC1 at approximately 87 kDa. The expected band size for DRC1 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09935-drc1-primary-antibodies-ihc-testing-1.jpgAnti-DRC1 Antibody Picoband®IHC analysis of DRC1 using anti-DRC1 antibody (A09935). DRC1 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DRC1 Antibody (A09935) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09935-drc1-primary-antibodies-ihc-testing-2.jpgAnti-DRC1 Antibody Picoband®IHC analysis of DRC1 using anti-DRC1 antibody (A09935). DRC1 was detected in a paraffin-embedded section of human fallopian tube tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DRC1 Antibody (A09935) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09935-drc1-primary-antibodies-ihc-testing-3.jpgAnti-DRC1 Antibody Picoband®IHC analysis of DRC1 using anti-DRC1 antibody (A09935). DRC1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DRC1 Antibody (A09935) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333852026-03-17T05:17:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05095-1-dsc3-primary-antibodies-wb-testing-1.jpgAnti-DSC3 Antibody Picoband®Western blot analysis of DSC3 using anti-DSC3 antibody (A05095-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human REH whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DSC3 antigen affinity purified polyclonal antibody (A05095-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DSC3 at approximately 90-110 kDa. The expected band size for DSC3 is at 100 kDa. https://www.bosterbio.com/catalog/product/view/id/1333862026-03-17T05:17:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03665-2-dscam-primary-antibodies-wb-testing-1.jpgAnti-DSCAM Antibody Picoband®Western blot analysis of DSCAM using anti-DSCAM antibody (A03665-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DSCAM antigen affinity purified polyclonal antibody (A03665-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DSCAM at approximately 300 kDa. The expected band size for DSCAM is at 222 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03665-2-dscam-primary-antibodies-if-testing-1.jpgAnti-DSCAM Antibody Picoband®IF analysis of DSCAM using anti-DSCAM antibody (A03665-2). DSCAM was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DSCAM Antibody (A03665-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03665-2-dscam-primary-antibodies-fcm-testing-1.jpgAnti-DSCAM Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-DSCAM antibody (A03665-2). Overlay histogram showing MCF-7 cells stained with A03665-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DSCAM Antibody (A03665-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333872026-03-17T05:17:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07949-2-dsn1-primary-antibodies-wb-testing-1.jpgAnti-DSN1 Antibody Picoband®Western blot analysis of DSN1 using anti-DSN1 antibody (A07949-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DSN1 antigen affinity purified polyclonal antibody (A07949-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DSN1 at approximately 38 kDa. The expected band size for DSN1 is at 38 kDa. https://www.bosterbio.com/catalog/product/view/id/1333882026-03-17T05:17:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02634-1-ugt1a4-primary-antibodies-wb-testing-1.jpgAnti-UGT1A4 Antibody Picoband®Western blot analysis of UGT1A4 using anti-UGT1A4 antibody (A02634-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UGT1A4 antigen affinity purified polyclonal antibody (A02634-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UGT1A4 at approximately 60 kDa. The expected band size for UGT1A4 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02634-1-ugt1a4-primary-antibodies-if-testing-1.jpgAnti-UGT1A4 Antibody Picoband®IF analysis of UGT1A4 using anti-UGT1A4 antibody (A02634-1) and anti-Alpha Tubulin antibody (M03989-3). UGT1A4 was detected in an immunocytochemical section of Hacat cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-UGT1A4 Antibody (A02634-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02634-1-ugt1a4-primary-antibodies-fcm-testing-1.jpgAnti-UGT1A4 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-UGT1A4 antibody (A02634-1). Overlay histogram showing U251 cells stained with A02634-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UGT1A4 Antibody (A02634-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333892026-03-17T05:17:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14467-1-dscr3-primary-antibodies-wb-testing-1.jpgAnti-VPS26C Antibody Picoband®Western blot analysis of DSCR3 using anti-DSCR3 antibody (A14467-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DSCR3 antigen affinity purified polyclonal antibody (A14467-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DSCR3 at approximately 36 kDa. The expected band size for DSCR3 is at 33 kDa. https://www.bosterbio.com/catalog/product/view/id/1333902026-03-17T05:17:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04765-2-eif3f-primary-antibodies-wb-testing-1.jpgAnti-EIF3F Antibody Picoband®Western blot analysis of EIF3F using anti-EIF3F antibody (A04765-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3F antigen affinity purified polyclonal antibody (A04765-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EIF3F at approximately 45 kDa. The expected band size for EIF3F is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04765-2-eif3f-primary-antibodies-ip-testing-1.jpgAnti-EIF3F Antibody Picoband®Immunoprecipitating EIF3F in U2OS whole cell lysate. Western blot analysis of EIF3F using anti-EIF3F antibody (A04765-2). Lane 1: U2OS whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-EIF3F antibody in U2OS whole cell lysate, Lane 3: anti-EIF3F antibody (2μg) + U2OS whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EIF3F antigen affinity purified polyclonal antibody (A04765-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EIF3F at approximately 45 kDa. The expected band size for EIF3F is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04765-2-eif3f-primary-antibodies-fcm-testing-1.jpgAnti-EIF3F Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-EIF3F antibody (A04765-2). Overlay histogram showing Jurkat cells stained with A04765-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF3F Antibody (A04765-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333912026-03-17T05:17:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14030-1-dimt1-primary-antibodies-wb-testing-1.jpgAnti-DIMT1 Antibody Picoband®Western blot analysis of DIMT1 using anti-DIMT1 antibody (A14030-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIMT1 antigen affinity purified polyclonal antibody (A14030-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DIMT1 at approximately 35 kDa. The expected band size for DIMT1 is at 35 kDa. https://www.bosterbio.com/catalog/product/view/id/1333922026-03-17T05:17:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09803-1-dip2a-primary-antibodies-wb-testing-1.jpgAnti-DIP2A Antibody Picoband®Western blot analysis of DIP2A using anti-DIP2A antibody (A09803-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIP2A antigen affinity purified polyclonal antibody (A09803-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DIP2A at approximately 110 kDa. The expected band size for DIP2A is at 170 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09803-1-dip2a-primary-antibodies-if-testing-1.jpgAnti-DIP2A Antibody Picoband®IF analysis of DIP2A using anti-DIP2A antibody (A09803-1) and anti-Alpha Tubulin antibody (M03989-3). DIP2A was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DIP2A Antibody (A09803-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09803-1-dip2a-primary-antibodies-fcm-testing-1.jpgAnti-DIP2A Antibody Picoband®Flow Cytometry analysis of A431 cells using anti-DIP2A antibody (A09803-1). Overlay histogram showing A431 cells stained with A09803-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DIP2A Antibody (A09803-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09803-1-dip2a-primary-antibodies-fcm-testing-2.jpgAnti-DIP2A Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-DIP2A antibody (A09803-1). Overlay histogram showing HEL cells stained with A09803-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DIP2A Antibody (A09803-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333932026-03-17T05:17:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07928-1-dlec1-primary-antibodies-wb-testing-1.jpgAnti-DLEC1 Antibody Picoband®Western blot analysis of DLEC1 using anti-DLEC1 antibody (A07928-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLEC1 antigen affinity purified polyclonal antibody (A07928-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DLEC1 at approximately 90 kDa. The expected band size for DLEC1 is at 196 kDa. https://www.bosterbio.com/catalog/product/view/id/1333942026-03-17T05:17:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10881-1-eef1g-primary-antibodies-wb-testing-1.jpgAnti-EEF1G Antibody Picoband®Western blot analysis of EEF1G using anti-EEF1G antibody (A10881-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEF1G antigen affinity purified polyclonal antibody (A10881-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EEF1G at approximately 50 kDa. The expected band size for EEF1G is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10881-1-eef1g-primary-antibodies-ihc-testing-1.jpgAnti-EEF1G Antibody Picoband®IHC analysis of EEF1G using anti-EEF1G antibody (A10881-1). EEF1G was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EEF1G Antibody (A10881-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10881-1-eef1g-primary-antibodies-ihc-testing-2.jpgAnti-EEF1G Antibody Picoband®IHC analysis of EEF1G using anti-EEF1G antibody (A10881-1). EEF1G was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EEF1G Antibody (A10881-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10881-1-eef1g-primary-antibodies-ihc-testing-3.jpgAnti-EEF1G Antibody Picoband®IHC analysis of EEF1G using anti-EEF1G antibody (A10881-1). EEF1G was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EEF1G Antibody (A10881-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10881-1-eef1g-primary-antibodies-ihc-testing-4.jpgAnti-EEF1G Antibody Picoband®IHC analysis of EEF1G using anti-EEF1G antibody (A10881-1). EEF1G was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EEF1G Antibody (A10881-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10881-1-eef1g-primary-antibodies-ip-testing-1.jpgAnti-EEF1G Antibody Picoband®Immunoprecipitating EEF1G in Hela whole cell lysate. Western blot analysis of EEF1G using anti-EEF1G antibody (A10881-1). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-EEF1G antibody in Hela whole cell lysate, Lane 3: anti-EEF1G antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EEF1G antigen affinity purified polyclonal antibody (A10881-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EEF1G at approximately 50 kDa. The expected band size for EEF1G is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10881-1-eef1g-primary-antibodies-fcm-testing-1.jpgAnti-EEF1G Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-EEF1G antibody (A10881-1). Overlay histogram showing MCF-7 cells stained with A10881-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EEF1G Antibody (A10881-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1333952026-03-17T05:17:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13604-1-eepd1-primary-antibodies-wb-testing-1.jpgAnti-EEPD1 Antibody Picoband®Western blot analysis of EEPD1 using anti-EEPD1 antibody (A13604-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-32 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat small intestine tissue lysates, Lane 5: mouse stomach tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEPD1 antigen affinity purified polyclonal antibody (A13604-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EEPD1 at approximately 63 kDa. The expected band size for EEPD1 is at 62 kDa. https://www.bosterbio.com/catalog/product/view/id/1333962026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1333972026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00284-2-rela-primary-antibodies-ihc-testing-1.jpgAnti-Phospho-NF-κB p65/RELA (S276) AntibodyIHC analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody (P00284-2). NF-κB p65/RELA was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NF-κB p65/RELA Antibody (P00284-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00284-2-rela-primary-antibodies-ihc-testing-2.jpgAnti-Phospho-NF-κB p65/RELA (S276) AntibodyIHC analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody (P00284-2). NF-κB p65/RELA was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NF-κB p65/RELA Antibody (P00284-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00284-2-rela-primary-antibodies-ihc-testing-3.jpgAnti-Phospho-NF-κB p65/RELA (S276) AntibodyIHC analysis of NF-κB p65/RELA using anti-NF-κB p65/RELA antibody (P00284-2). NF-κB p65/RELA was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NF-κB p65/RELA Antibody (P00284-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333982026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00024-7-3-primary-antibodies-ihc-testing-1.jpgAnti-Phospho-AKT1/2/3 (S473) AntibodyIHC analysis of AKT1/2/3 using anti-AKT1/2/3 antibody (P00024-7). AKT1/2/3 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-AKT1/2/3 Antibody (P00024-7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1333992026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334002026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334012026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334022026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334032026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334042026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334052026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334062026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334072026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334082026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334092026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334102026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334112026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334122026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334132026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334142026-03-16T05:11:22+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334152026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334162026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334172026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334182026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334192026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334202026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334212026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334222026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334232026-03-16T05:11:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00176-2-p-ampk14-primary-antibodies-wb-testing-1.jpgAnti-Phospho-p38 MAPK/MAPK14 (Y182) AntibodyWestern blot analysis of P-AMPK14(T178,T182) using anti-P-AMPK14(T178,T182) antibody (P00176-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse Nruro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-AMPK14(T178,T182) antigen affinity purified monoclonal antibody (P00176-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-AMPK14(T178,T182) at approximately 44 kDa. The expected band size for P-AMPK14(T178,T182) is at 41 kDa. https://www.bosterbio.com/catalog/product/view/id/1334242026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334252026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334262026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334272026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334282026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334292026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334302026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334312026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334322026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334332026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334342026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334352026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334362026-03-16T05:11:23+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334372026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334382026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334392026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334402026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334412026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334422026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00090-3-smad2-primary-antibodies-ihc-testing-1.jpgAnti-Phospho-SMAD2 (S465+S467) AntibodyIHC analysis of SMAD2 using anti-SMAD2 antibody (P00090-3). SMAD2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SMAD2 Antibody (P00090-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00090-3-smad2-primary-antibodies-ihc-testing-2.jpgAnti-Phospho-SMAD2 (S465+S467) AntibodyIHC analysis of SMAD2 using anti-SMAD2 antibody (P00090-3). SMAD2 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SMAD2 Antibody (P00090-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00090-3-smad2-primary-antibodies-ihc-testing-3.jpgAnti-Phospho-SMAD2 (S465+S467) AntibodyIHC analysis of SMAD2 using anti-SMAD2 antibody (P00090-3). SMAD2 was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SMAD2 Antibody (P00090-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00090-3-smad2-primary-antibodies-ihc-testing-4.jpgAnti-Phospho-SMAD2 (S465+S467) AntibodyIHC analysis of SMAD2 using anti-SMAD2 antibody (P00090-3). SMAD2 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SMAD2 Antibody (P00090-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1334432026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334442026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334452026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334462026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334472026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334482026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334492026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334502026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334512026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334522026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334532026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334542026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334552026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334562026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334572026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334582026-03-16T05:11:24+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334592026-03-16T05:11:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334602026-03-16T05:11:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334612026-03-16T05:11:25+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1334622026-03-16T05:11:25+00:00daily0.9 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https://www.bosterbio.com/catalog/product/view/id/1335952026-03-16T05:11:31+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1335962026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00097-4-mapt-primary-antibodies-wb-testing-1.jpgAnti-Phospho-TAU/MAPT (T205) AntibodyWestern blot analysis of TAU/MAPT using anti-TAU/MAPT antibody (P00097-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAU/MAPT antigen affinity purified polyclonal antibody (P00097-4) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAU/MAPT at approximately 80 kDa. 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https://www.bosterbio.com/catalog/product/view/id/1336512026-03-16T05:11:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1336522026-03-16T05:11:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1336532026-03-16T05:11:34+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1336542026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00340-1-nfatc1-primary-antibodies-wb-testing-1.jpgAnti-Phospho-NFATC1 (S294) AntibodyWestern blot analysis of NFATC1 using anti-NFATC1 antibody (P00340-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFATC1 antigen affinity purified polyclonal antibody (P00340-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NFATC1 at approximately 101 kDa. 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https://www.bosterbio.com/catalog/product/view/id/1338632026-03-16T05:11:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338642026-03-16T05:11:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338652026-03-16T05:11:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338662026-03-16T05:11:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338672026-03-16T05:11:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338682026-03-16T05:11:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338692026-03-16T05:11:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338702026-03-16T05:11:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338712026-03-16T05:11:45+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338722026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338732026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338742026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338752026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338762026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338772026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338782026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338792026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338802026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338812026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338822026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338832026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338842026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338852026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338862026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338872026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338882026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338892026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338902026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338912026-03-16T05:11:46+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338922026-03-16T05:11:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338932026-03-16T05:11:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338942026-03-16T05:11:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338952026-03-16T05:11:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338962026-03-16T05:11:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1338972026-03-10T04:43:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0772.pngMouse SPINT1/HAI-1 PicoKine&reg Quick ELISA KitMouse SPINT1/HAI-1 PicoKine Quick ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1338982026-03-10T04:43:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0578.pngHuman Growth Hormone/GH1 PicoKine® Quick ELISA KitHuman Growth Hormone PicoKine Quick ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1339032026-03-10T04:43:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1339042026-03-10T04:43:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1339052026-03-10T04:43:54+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1339062026-03-10T04:43:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1339072026-03-10T04:43:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1339082026-03-10T04:43:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1339092026-03-10T04:43:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1339102026-03-10T04:43:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1339112026-03-10T04:43:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1339122026-03-10T04:43:55+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1339132026-03-10T04:43:55+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dcp2-picoband-antibody-azq803b9-boster.html2026-03-17T05:17:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq803b9-dcp2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DCP2 Antibody Picoband®Western blot analysis of DCP2 using anti-DCP2 antibody (AZQ803B9). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCP2 antigen affinity purified polyclonal antibody (AZQ803B9) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCP2 at approximately 48 kDa. The expected band size for DCP2 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq803b9-dcp2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish DCP2 Antibody Picoband®IHC analysis of DCP2 using anti-DCP2 antibody (AZQ803B9). DCP2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DCP2 Antibody (AZQ803B9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq803b9-dcp2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish DCP2 Antibody Picoband®IHC analysis of DCP2 using anti-DCP2 antibody (AZQ803B9). DCP2 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DCP2 Antibody (AZQ803B9) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dapk2a-picoband-antibody-aze7f1a1-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f1a1-dapk2a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DAPK2A Antibody Picoband®Western blot analysis of DAPK2A using anti-DAPK2A antibody (AZE7F1A1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAPK2A antigen affinity purified polyclonal antibody (AZE7F1A1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DAPK2A at approximately 56 kDa. The expected band size for DAPK2A is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f1a1-dapk2a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish DAPK2A Antibody Picoband®IHC analysis of DAPK2A using anti-DAPK2A antibody (AZE7F1A1). DAPK2A was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAPK2A Antibody (AZE7F1A1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aze7f1a1-dapk2a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish DAPK2A Antibody Picoband®IHC analysis of DAPK2A using anti-DAPK2A antibody (AZE7F1A1). DAPK2A was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DAPK2A Antibody (AZE7F1A1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ddit4-picoband-antibody-azq7t346-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7t346-ddit4-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DDIT4 Antibody Picoband®Western blot analysis of DDIT4 using anti-DDIT4 antibody (AZQ7T346). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDIT4 antigen affinity purified polyclonal antibody (AZQ7T346) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDIT4 at approximately 24 kDa. The expected band size for DDIT4 is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-notch1a-picoband-antibody-azp46530-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp46530-notch1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NOTCH1A Antibody Picoband®Western blot analysis of NOTCH1A using anti-NOTCH1A antibody (AZP46530). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOTCH1A antigen affinity purified polyclonal antibody (AZP46530) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NOTCH1A at approximately 262 kDa. The expected band size for NOTCH1A is at 262 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lcp1-picoband-antibody-azq6p698-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p698-lcp1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LCP1 Antibody Picoband®Western blot analysis of LCP1 using anti-LCP1 antibody (AZQ6P698). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCP1 antigen affinity purified polyclonal antibody (AZQ6P698) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LCP1 at approximately 70 kDa. The expected band size for LCP1 is at 70 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nppa-picoband-antibody-azf1qvw0-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1qvw0-nppa-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NPPA Antibody Picoband®Western blot analysis of NPPA using anti-NPPA antibody (AZF1QVW0). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPPA antigen affinity purified polyclonal antibody (AZF1QVW0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NPPA at approximately 15 kDa. The expected band size for NPPA is at 15 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-thraa-picoband-antibody-azq98867-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq98867-thraa-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish THRAA Antibody Picoband®Western blot analysis of THRAA using anti-THRAA antibody (AZQ98867). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THRAA antigen affinity purified polyclonal antibody (AZQ98867) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for THRAA at approximately 52 kDa. The expected band size for THRAA is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-lft2-picoband-antibody-azq9w6i7-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9w6i7-lft2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish LFT2 Antibody Picoband®Western blot analysis of LFT2 using anti-LFT2 antibody (AZQ9W6I7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LFT2 antigen affinity purified polyclonal antibody (AZQ9W6I7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LFT2 at approximately 41 kDa. The expected band size for LFT2 is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-drd4a-picoband-antibody-aza0a0r4ir92-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4ir92-drd4a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DRD4A Antibody Picoband®Western blot analysis of DRD4A using anti-DRD4A antibody (AZA0A0R4IR92). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRD4A antigen affinity purified polyclonal antibody (AZA0A0R4IR92) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DRD4A at approximately 43 kDa. The expected band size for DRD4A is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hmgcs1-picoband-antibody-aza0a0r4iqg1-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4iqg1-hmgcs1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HMGCS1 Antibody Picoband®Western blot analysis of HMGCS1 using anti-HMGCS1 antibody (AZA0A0R4IQG1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGCS1 antigen affinity purified polyclonal antibody (AZA0A0R4IQG1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HMGCS1 at approximately 53 kDa. The expected band size for HMGCS1 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4iqg1-hmgcs1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HMGCS1 Antibody Picoband®IHC analysis of HMGCS1 using anti-HMGCS1 antibody (AZA0A0R4IQG1). HMGCS1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMGCS1 Antibody (AZA0A0R4IQG1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dio3a-antibody-aza0a8m1p485-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1p485-dio3a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish DIO3A AntibodyIHC analysis of DIO3A using anti-DIO3A antibody (AZA0A8M1P485). DIO3A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DIO3A Antibody (AZA0A8M1P485) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1p485-dio3a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish DIO3A AntibodyIHC analysis of DIO3A using anti-DIO3A antibody (AZA0A8M1P485). DIO3A was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DIO3A Antibody (AZA0A8M1P485) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cdt1-picoband-antibody-aza0a8m6yzj1-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yzj1-cdt1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CDT1 Antibody Picoband®Western blot analysis of CDT1 using anti-CDT1 antibody (AZA0A8M6YZJ1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDT1 antigen affinity purified polyclonal antibody (AZA0A8M6YZJ1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CDT1 at approximately 60 kDa. The expected band size for CDT1 is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-kiss2-picoband-antibody-azb6ziv2-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb6ziv2-kiss2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish KISS2 Antibody Picoband®Western blot analysis of KISS2 using anti-KISS2 antibody (AZB6ZIV2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KISS2 antigen affinity purified polyclonal antibody (AZB6ZIV2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KISS2 at approximately 14 kDa. The expected band size for KISS2 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb6ziv2-kiss2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish KISS2 Antibody Picoband®IHC analysis of KISS2 using anti-KISS2 antibody (AZB6ZIV2). KISS2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KISS2 Antibody (AZB6ZIV2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb6ziv2-kiss2-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish KISS2 Antibody Picoband®IHC analysis of KISS2 using anti-KISS2 antibody (AZB6ZIV2). KISS2 was detected in a paraffin-embedded section of zebrafish brain1 tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KISS2 Antibody (AZB6ZIV2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-epha2a-picoband-antibody-azq6nzs1-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nzs1-epha2a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish EPHA2A Antibody Picoband®Western blot analysis of EPHA2A using anti-EPHA2A antibody (AZQ6NZS1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHA2A antigen affinity purified polyclonal antibody (AZQ6NZS1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPHA2A at approximately 109 kDa. The expected band size for EPHA2A is at 109 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gdf9-antibody-azq5md90-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5md90-gdf9-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish GDF9 AntibodyIHC analysis of GDF9 using anti-GDF9 antibody (AZQ5MD90). GDF9 was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GDF9 Antibody (AZQ5MD90) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-foxd1-antibody-aza2ce80-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2ce80-foxd1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish FOXD1 AntibodyIHC analysis of FOXD1 using anti-FOXD1 antibody (AZA2CE80). FOXD1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXD1 Antibody (AZA2CE80) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2ce80-foxd1-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish FOXD1 AntibodyIHC analysis of FOXD1 using anti-FOXD1 antibody (AZA2CE80). FOXD1 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXD1 Antibody (AZA2CE80) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-foxe3-antibody-azb0uxi3-boster.html2026-03-17T05:17:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0uxi3-foxe3-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish FOXE3 AntibodyIHC analysis of FOXE3 using anti-FOXE3 antibody (AZB0UXI3). FOXE3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXE3 Antibody (AZB0UXI3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0uxi3-foxe3-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish FOXE3 AntibodyIHC analysis of FOXE3 using anti-FOXE3 antibody (AZB0UXI3). FOXE3 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXE3 Antibody (AZB0UXI3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hoxa10b-picoband-antibody-azq8awy2-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8awy2-hoxa10b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HOXA10B Antibody Picoband®Western blot analysis of HOXA10B using anti-HOXA10B antibody (AZQ8AWY2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA10B antigen affinity purified polyclonal antibody (AZQ8AWY2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HOXA10B at approximately 37 kDa. The expected band size for HOXA10B is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-hoxa3-3a-picoband-antibody-azq8awz2-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8awz2-hoxa3a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish HOXA3/3A Antibody Picoband®Western blot analysis of HOXA3A using anti-HOXA3A antibody (AZQ8AWZ2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA3A antigen affinity purified polyclonal antibody (AZQ8AWZ2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HOXA3A at approximately 50 kDa. The expected band size for HOXA3A is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8awz2-hoxa3a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish HOXA3/3A Antibody Picoband®IHC analysis of HOXA3A using anti-HOXA3A antibody (AZQ8AWZ2). HOXA3A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXA3A Antibody (AZQ8AWZ2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8awz2-hoxa3a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish HOXA3/3A Antibody Picoband®IHC analysis of HOXA3A using anti-HOXA3A antibody (AZQ8AWZ2). HOXA3A was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HOXA3A Antibody (AZQ8AWZ2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mc4r-picoband-antibody-azb0v1p1-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0v1p1-mc4r-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MC4R Antibody Picoband®Western blot analysis of MC4R using anti-MC4R antibody (AZB0V1P1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MC4R antigen affinity purified polyclonal antibody (AZB0V1P1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MC4R at approximately 40 kDa. The expected band size for MC4R is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0v1p1-mc4r-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish MC4R Antibody Picoband®IHC analysis of MC4R using anti-MC4R antibody (AZB0V1P1). MC4R was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MC4R Antibody (AZB0V1P1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0v1p1-mc4r-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish MC4R Antibody Picoband®IHC analysis of MC4R using anti-MC4R antibody (AZB0V1P1). MC4R was detected in a paraffin-embedded section of zebrafish ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MC4R Antibody (AZB0V1P1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-rad51-picoband-antibody-azq5tyr1-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5tyr1-rad51-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish RAD51 Antibody Picoband®Western blot analysis of RAD51 using anti-RAD51 antibody (AZQ5TYR1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51 antigen affinity purified polyclonal antibody (AZQ5TYR1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAD51 at approximately 38 kDa. The expected band size for RAD51 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5tyr1-rad51-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish RAD51 Antibody Picoband®IHC analysis of RAD51 using anti-RAD51 antibody (AZQ5TYR1). RAD51 was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAD51 Antibody (AZQ5TYR1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-stat1a-picoband-antibody-azo93598-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azo93598-stat1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish STAT1A Antibody Picoband®Western blot analysis of STAT1A using anti-STAT1A antibody (AZO93598). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1A antigen affinity purified polyclonal antibody (AZO93598) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STAT1A at approximately 86 kDa. The expected band size for STAT1A is at 86 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-stat5a-picoband-antibody-azq68sp3-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq68sp3-stat5a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish STAT5A Antibody Picoband®Western blot analysis of STAT5A using anti-STAT5A antibody (AZQ68SP3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A antigen affinity purified polyclonal antibody (AZQ68SP3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STAT5A at approximately 87 kDa. The expected band size for STAT5A is at 87 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dgat1a-picoband-antibody-azq6p3j0-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p3j0-dgat1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DGAT1A Antibody Picoband®Western blot analysis of DGAT1A using anti-DGAT1A antibody (AZQ6P3J0). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DGAT1A antigen affinity purified polyclonal antibody (AZQ6P3J0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DGAT1A at approximately 50 kDa. The expected band size for DGAT1A is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-col1a1a-picoband-antibody-azq6u1j5-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6u1j5-col1a1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish COL1A1A Antibody Picoband®Western blot analysis of COL1A1A using anti-COL1A1A antibody (AZQ6U1J5). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL1A1A antigen affinity purified polyclonal antibody (AZQ6U1J5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for COL1A1A at approximately 137 kDa. The expected band size for COL1A1A is at 137 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ikzf1-picoband-antibody-aza0a8m2b2x4-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2b2x4-ikzf1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish IKZF1 Antibody Picoband®Western blot analysis of IKZF1 using anti-IKZF1 antibody (AZA0A8M2B2X4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKZF1 antigen affinity purified polyclonal antibody (AZA0A8M2B2X4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IKZF1 at approximately 59 kDa. The expected band size for IKZF1 is at 59 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dnmt1-picoband-antibody-aza0a8m6yux4-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m6yux4-dnmt1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DNMT1 Antibody Picoband®Western blot analysis of DNMT1 using anti-DNMT1 antibody (AZA0A8M6YUX4). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT1 antigen affinity purified polyclonal antibody (AZA0A8M6YUX4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNMT1 at approximately 169 kDa. The expected band size for DNMT1 is at 169 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-col10a1a-antibody-aza2rqr3-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2rqr3-col10a1a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish COL10A1A AntibodyIHC analysis of COL10A1A using anti-COL10A1A antibody (AZA2RQR3). COL10A1A was detected in a paraffin-embedded section of zebrafish tooth tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL10A1A Antibody (AZA2RQR3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2rqr3-col10a1a-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish COL10A1A AntibodyIHC analysis of COL10A1A using anti-COL10A1A antibody (AZA2RQR3). COL10A1A was detected in a paraffin-embedded section of zebrafish vertebra tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL10A1A Antibody (AZA2RQR3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza2rqr3-col10a1a-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish COL10A1A AntibodyIHC analysis of COL10A1A using anti-COL10A1A antibody (AZA2RQR3). COL10A1A was detected in a paraffin-embedded section of zebrafish gill tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL10A1A Antibody (AZA2RQR3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dock1-picoband-antibody-aza8e1v6-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8e1v6-dock1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DOCK1 Antibody Picoband®Western blot analysis of DOCK1 using anti-DOCK1 antibody (AZA8E1V6). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOCK1 antigen affinity purified polyclonal antibody (AZA8E1V6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DOCK1 at approximately 230 kDa. The expected band size for DOCK1 is at 215 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dock4a-picoband-antibody-aza0a8m9peg0-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9peg0-dock4a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DOCK4A Antibody Picoband®Western blot analysis of DOCK4A using anti-DOCK4A antibody (AZA0A8M9PEG0). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DOCK4A antigen affinity purified polyclonal antibody (AZA0A8M9PEG0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DOCK4A at approximately 221 kDa. The expected band size for DOCK4A is at 221 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fzd2-picoband-antibody-azq90yl7-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90yl7-fzd2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FZD2 Antibody Picoband®Western blot analysis of FZD2 using anti-FZD2 antibody (AZQ90YL7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FZD2 antigen affinity purified polyclonal antibody (AZQ90YL7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FZD2 at approximately 70 kDa. The expected band size for FZD2 is at 63 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fzd3a-antibody-aza0a8m2b7p5-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m2b7p5-fzd3a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish FZD3A AntibodyIHC analysis of FZD3A using anti-FZD3A antibody (AZA0A8M2B7P5). FZD3A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FZD3A Antibody (AZA0A8M2B7P5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fzd8a-antibody-azq9yi00-boster.html2026-03-17T05:17:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9yi00-fzd8a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish FZD8A AntibodyIHC analysis of FZD8A using anti-FZD8A antibody (AZQ9YI00). FZD8A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FZD8A Antibody (AZQ9YI00) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fzd9a-antibody-aza0a8m1rf02-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1rf02-fzd9a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish FZD9A AntibodyIHC analysis of FZD9A using anti-FZD9A antibody (AZA0A8M1RF02). FZD9A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FZD9A Antibody (AZA0A8M1RF02) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gnrhr4-antibody-aza3qjz0-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza3qjz0-gnrhr4-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish GNRHR4 AntibodyIHC analysis of GNRHR4 using anti-GNRHR4 antibody (AZA3QJZ0). GNRHR4 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GNRHR4 Antibody (AZA3QJZ0) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-nkx2-1-picoband-antibody-azq98tx4-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq98tx4-nkx2-1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish NKX2.1 Antibody Picoband®Western blot analysis of NKX2.1 using anti-NKX2.1 antibody (AZQ98TX4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKX2.1 antigen affinity purified polyclonal antibody (AZQ98TX4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NKX2.1 at approximately 42 kDa. The expected band size for NKX2.1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq98tx4-nkx2-1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish NKX2.1 Antibody Picoband®IHC analysis of NKX2.1 using anti-NKX2.1 antibody (AZQ98TX4). NKX2.1 was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NKX2.1 Antibody (AZQ98TX4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-aldh1a3-picoband-antibody-azq0h2g3-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq0h2g3-aldh1a3-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ALDH1A3 Antibody Picoband®Western blot analysis of ALDH1A3 using anti-ALDH1A3 antibody (AZQ0H2G3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1A3 antigen affinity purified polyclonal antibody (AZQ0H2G3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALDH1A3 at approximately 56 kDa. The expected band size for ALDH1A3 is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-aldh2-1-2-2-picoband-antibody-azq7sxu3-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxu3-aldh2-1-2-2-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ALDH2.1/2.2 Antibody Picoband®Western blot analysis of ALDH2.1,2.2 using anti-ALDH2.1,2.2 antibody (AZQ7SXU3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH2.1,2.2 antigen affinity purified polyclonal antibody (AZQ7SXU3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALDH2.1,2.2 at approximately 56 kDa. The expected band size for ALDH2.1,2.2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7sxu3-aldh2-1-2-2-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish ALDH2.1/2.2 Antibody Picoband®IHC analysis of ALDH2.1,2.2 using anti-ALDH2.1,2.2 antibody (AZQ7SXU3). ALDH2.1,2.2 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH2.1,2.2 Antibody (AZQ7SXU3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-igf3-antibody-azb0z6g1-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azb0z6g1-igf3-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish IGF3 AntibodyIHC analysis of IGF3 using anti-IGF3 antibody (AZB0Z6G1). IGF3 was detected in a paraffin-embedded section of zebrafish testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF3 Antibody (AZB0Z6G1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mmp19-antibody-aza0ab32tz88-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0ab32tz88-mmp19-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish MMP19 AntibodyIHC analysis of MMP19 using anti-MMP19 antibody (AZA0AB32TZ88). MMP19 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP19 Antibody (AZA0AB32TZ88) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cd36-antibody-azq6dhc7-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6dhc7-cd36-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish CD36 AntibodyIHC analysis of CD36 using anti-CD36 antibody (AZQ6DHC7). CD36 was detected in a paraffin-embedded section of zebrafish small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD36 Antibody (AZQ6DHC7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-alpha-a-crystallin-cryaa-picoband-antibody-azq8uuz6-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8uuz6-cryaa-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Alpha A Crystallin/CRYAA Antibody Picoband®Western blot analysis of Alpha A Crystallin/CRYAA using anti-Alpha A Crystallin/CRYAA antibody (AZQ8UUZ6). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha A Crystallin/CRYAA antigen affinity purified polyclonal antibody (AZQ8UUZ6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Alpha A Crystallin/CRYAA at approximately 20 kDa. The expected band size for Alpha A Crystallin/CRYAA is at 20 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-p0-mpz-picoband-antibody-aza0a0r4is04-boster.html2026-03-24T05:36:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4is04-mpz-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish P0/MPZ Antibody Picoband®Western blot analysis of P0/MPZ using anti-P0/MPZ antibody (AZA0A0R4IS04). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P0/MPZ antigen affinity purified polyclonal antibody (AZA0A0R4IS04) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P0/MPZ at approximately 22 kDa. The expected band size for P0/MPZ is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4is04-mpz-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish P0/MPZ Antibody Picoband®IHC analysis of P0/MPZ using anti-P0/MPZ antibody (AZA0A0R4IS04). P0/MPZ was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P0/MPZ Antibody (AZA0A0R4IS04) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4is04-mpz-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish P0/MPZ Antibody Picoband®IHC analysis of P0/MPZ using anti-P0/MPZ antibody (AZA0A0R4IS04). P0/MPZ was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P0/MPZ Antibody (AZA0A0R4IS04) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4is04-mpz-primary-antibodies-if-testing-1.jpgAnti-Zebrafish P0/MPZ Antibody Picoband®IF analysis of P0/MPZ using anti-P0/MPZ antibody (AZA0A0R4IS04). P0/MPZ was detected in a paraffin-embedded section of adult zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-P0/MPZ Antibody (AZA0A0R4IS04) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4is04-mpz-primary-antibodies-if-testing-2.jpgAnti-Zebrafish P0/MPZ Antibody Picoband®IF analysis of P0/MPZ using anti-P0/MPZ antibody (AZA0A0R4IS04). P0/MPZ was detected in a paraffin-embedded section of adult zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-P0/MPZ Antibody (AZA0A0R4IS04) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-vhl-picoband-antibody-aza1l296-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l296-vhl-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish VHL Antibody Picoband®Western blot analysis of VHL using anti-VHL antibody (AZA1L296). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VHL antigen affinity purified polyclonal antibody (AZA1L296) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VHL at approximately 18-24 kDa. The expected band size for VHL is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza1l296-vhl-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish VHL Antibody Picoband®IHC analysis of VHL using anti-VHL antibody (AZA1L296). VHL was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VHL Antibody (AZA1L296) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ppiaa-picoband-antibody-aza0a8m1pht1-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m1pht1-ppiaa-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PPIAA Antibody Picoband®Western blot analysis of PPIAA using anti-PPIAA antibody (AZA0A8M1PHT1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPIAA antigen affinity purified polyclonal antibody (AZA0A8M1PHT1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PPIAA at approximately 17 kDa. The expected band size for PPIAA is at 17 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-wnt8a-picoband-antibody-azp51028-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp51028-wnt8a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish WNT8A Antibody Picoband®Western blot analysis of WNT8A using anti-WNT8A antibody (AZP51028). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT8A antigen affinity purified polyclonal antibody (AZP51028) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for WNT8A at approximately 42 kDa. The expected band size for WNT8A is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azp51028-wnt8a-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish WNT8A Antibody Picoband®IHC analysis of WNT8A using anti-WNT8A antibody (AZP51028). WNT8A was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WNT8A Antibody (AZP51028) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fabp6-picoband-antibody-azq6imw5-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6imw5-fabp6-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FABP6 Antibody Picoband®Western blot analysis of FABP6 using anti-FABP6 antibody (AZQ6IMW5). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP6 antigen affinity purified polyclonal antibody (AZQ6IMW5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FABP6 at approximately 14 kDa. The expected band size for FABP6 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6imw5-fabp6-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish FABP6 Antibody Picoband®IHC analysis of FABP6 using anti-FABP6 antibody (AZQ6IMW5). FABP6 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FABP6 Antibody (AZQ6IMW5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-irf6-picoband-antibody-azq6pgz7-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pgz7-irf6-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish IRF6 Antibody Picoband®Western blot analysis of IRF6 using anti-IRF6 antibody (AZQ6PGZ7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF6 antigen affinity purified polyclonal antibody (AZQ6PGZ7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IRF6 at approximately 60 kDa. The expected band size for IRF6 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6pgz7-irf6-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish IRF6 Antibody Picoband®IHC analysis of IRF6 using anti-IRF6 antibody (AZQ6PGZ7). IRF6 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRF6 Antibody (AZQ6PGZ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-asc-tms1-pycard-picoband-antibody-azq9i9n6-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i9n6-pycard-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish ASC/TMS1/PYCARD Antibody Picoband®Western blot analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (AZQ9I9N6). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC/TMS1/PYCARD antigen affinity purified polyclonal antibody (AZQ9I9N6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ASC/TMS1/PYCARD at approximately 23 kDa. The expected band size for ASC/TMS1/PYCARD is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9i9n6-pycard-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish ASC/TMS1/PYCARD Antibody Picoband®IHC analysis of ASC/TMS1/PYCARD using anti-ASC/TMS1/PYCARD antibody (AZQ9I9N6). ASC/TMS1/PYCARD was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ASC/TMS1/PYCARD Antibody (AZQ9I9N6) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dpyd-picoband-antibody-azq6nyg8-boster.html2026-03-17T05:17:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nyg8-dpyd-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DPYD Antibody Picoband®Western blot analysis of DPYD using anti-DPYD antibody (AZQ6NYG8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPYD antigen affinity purified polyclonal antibody (AZQ6NYG8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DPYD at approximately 111 kDa. The expected band size for DPYD is at 111 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6nyg8-dpyd-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish DPYD Antibody Picoband®IHC analysis of DPYD using anti-DPYD antibody (AZQ6NYG8). DPYD was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DPYD Antibody (AZQ6NYG8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mkp-1-dusp1-picoband-antibody-azq7zvl8-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq7zvl8-dusp1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MKP-1/DUSP1 Antibody Picoband®Western blot analysis of MKP-1/DUSP1 using anti-MKP-1/DUSP1 antibody (AZQ7ZVL8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKP-1/DUSP1 antigen affinity purified polyclonal antibody (AZQ7ZVL8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MKP-1/DUSP1 at approximately 40 kDa. The expected band size for MKP-1/DUSP1 is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dtnba-picoband-antibody-aza0a0r4iav8-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4iav8-dtnba-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DTNBA Antibody Picoband®Western blot analysis of DTNBA using anti-DTNBA antibody (AZA0A0R4IAV8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DTNBA antigen affinity purified polyclonal antibody (AZA0A0R4IAV8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DTNBA at approximately 66 kDa. The expected band size for DTNBA is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4iav8-dtnba-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish DTNBA Antibody Picoband®IHC analysis of DTNBA using anti-DTNBA antibody (AZA0A0R4IAV8). DTNBA was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DTNBA Antibody (AZA0A0R4IAV8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dmpk-picoband-antibody-aza0a8m3b300-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3b300-dmpk-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DMPK Antibody Picoband®Western blot analysis of DMPK using anti-DMPK antibody (AZA0A8M3B300). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DMPK antigen affinity purified polyclonal antibody (AZA0A8M3B300) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DMPK at approximately 67 kDa. The expected band size for DMPK is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3b300-dmpk-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish DMPK Antibody Picoband®IHC analysis of DMPK using anti-DMPK antibody (AZA0A8M3B300). DMPK was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DMPK Antibody (AZA0A8M3B300) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3b300-dmpk-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish DMPK Antibody Picoband®IHC analysis of DMPK using anti-DMPK antibody (AZA0A8M3B300). DMPK was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DMPK Antibody (AZA0A8M3B300) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m3b300-dmpk-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish DMPK Antibody Picoband®IHC analysis of DMPK using anti-DMPK antibody (AZA0A8M3B300). DMPK was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DMPK Antibody (AZA0A8M3B300) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dse-picoband-antibody-azq5rgl8-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq5rgl8-dse-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DSE Antibody Picoband®Western blot analysis of DSE using anti-DSE antibody (AZQ5RGL8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates, Lane 2: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DSE antigen affinity purified polyclonal antibody (AZQ5RGL8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DSE at approximately 180 kDa. The expected band size for DSE is at 110 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-dut-picoband-antibody-aza0a8m9p120-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a8m9p120-dut-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DUT Antibody Picoband®Western blot analysis of DUT using anti-DUT antibody (AZA0A8M9P120). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUT antigen affinity purified polyclonal antibody (AZA0A8M9P120) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DUT at approximately 20 kDa. The expected band size for DUT is at 20 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-eef1a1a-1b-picoband-antibody-azq6p969-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p969-eef1a1a-1b-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish EEF1A1A/1B Antibody Picoband®Western blot analysis of EEF1A1A/1B using anti-EEF1A1A/1B antibody (AZQ6P969). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEF1A1A/1B antigen affinity purified polyclonal antibody (AZQ6P969) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EEF1A1A/1B at approximately 50 kDa. The expected band size for EEF1A1A/1B is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p969-eef1a1a-1b-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish EEF1A1A/1B Antibody Picoband®IHC analysis of EEF1A1A/1B using anti-EEF1A1A/1B antibody (AZQ6P969). EEF1A1A/1B was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EEF1A1A/1B Antibody (AZQ6P969) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p969-eef1a1a-1b-primary-antibodies-ihc-testing-2.jpgAnti-Zebrafish EEF1A1A/1B Antibody Picoband®IHC analysis of EEF1A1A/1B using anti-EEF1A1A/1B antibody (AZQ6P969). EEF1A1A/1B was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EEF1A1A/1B Antibody (AZQ6P969) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq6p969-eef1a1a-1b-primary-antibodies-ihc-testing-3.jpgAnti-Zebrafish EEF1A1A/1B Antibody Picoband®IHC analysis of EEF1A1A/1B using anti-EEF1A1A/1B antibody (AZQ6P969). EEF1A1A/1B was detected in a paraffin-embedded section of zebrafish skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EEF1A1A/1B Antibody (AZQ6P969) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-ehf-picoband-antibody-azq29r87-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq29r87-ehf-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish EHF Antibody Picoband®Western blot analysis of EHF using anti-EHF antibody (AZQ29R87). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EHF antigen affinity purified polyclonal antibody (AZQ29R87) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EHF at approximately 33 kDa. The expected band size for EHF is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-tpm1-picoband-antibody-aza0a1l6uw63-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a1l6uw63-tpm1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish TPM1 Antibody Picoband®Western blot analysis of TPM1 using anti-TPM1 antibody (AZA0A1L6UW63). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPM1 antigen affinity purified polyclonal antibody (AZA0A1L6UW63) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TPM1 at approximately 36 kDa. The expected band size for TPM1 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a1l6uw63-tpm1-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish TPM1 Antibody Picoband®IHC analysis of TPM1 using anti-TPM1 antibody (AZA0A1L6UW63). TPM1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TPM1 Antibody (AZA0A1L6UW63) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-desma-picoband-antibody-azf1r8w4-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1r8w4-desma-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish DESMA Antibody Picoband®Western blot analysis of DESMA using anti-DESMA antibody (AZF1R8W4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DESMA antigen affinity purified polyclonal antibody (AZF1R8W4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DESMA at approximately 50 kDa. The expected band size for DESMA is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-vimentin-vim-picoband-antibody-azq9yhx5-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9yhx5-vim-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Vimentin/VIM Antibody Picoband®Western blot analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (AZQ9YHX5). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin/VIM antigen affinity purified polyclonal antibody (AZQ9YHX5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Vimentin/VIM at approximately 54 kDa. The expected band size for Vimentin/VIM is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9yhx5-vim-primary-antibodies-ihc-testing-1.jpgAnti-Zebrafish Vimentin/VIM Antibody Picoband®IHC analysis of Vimentin/VIM using anti-Vimentin/VIM antibody (AZQ9YHX5). Vimentin/VIM was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vimentin/VIM Antibody (AZQ9YHX5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gja5a-picoband-antibody-azf1ql21-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azf1ql21-gja5a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GJA5A Antibody Picoband®Western blot analysis of GJA5A using anti-GJA5A antibody (AZF1QL21). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA5A antigen affinity purified polyclonal antibody (AZF1QL21) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GJA5A at approximately 45 kDa. The expected band size for GJA5A is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fsta-picoband-antibody-azq9yhv4-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9yhv4-fsta-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FSTA Antibody Picoband®Western blot analysis of FSTA using anti-FSTA antibody (AZQ9YHV4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSTA antigen affinity purified polyclonal antibody (AZQ9YHV4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FSTA at approximately 38 kDa. The expected band size for FSTA is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-yap1-picoband-antibody-azq1l8j7-boster.html2026-03-17T05:17:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq1l8j7-yap1-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish YAP1 Antibody Picoband®Western blot analysis of YAP1 using anti-YAP1 antibody (AZQ1L8J7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YAP1 antigen affinity purified polyclonal antibody (AZQ1L8J7) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-pkc-iota-prkci-picoband-antibody-azq90xf2-boster.html2026-03-17T05:17:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90xf2-prkci-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish PKC Iota/PRKCI Antibody Picoband®Western blot analysis of PKC Iota/PRKCI using anti-PKC Iota/PRKCI antibody (AZQ90XF2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC Iota/PRKCI antigen affinity purified polyclonal antibody (AZQ90XF2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PKC Iota/PRKCI at approximately 67 kDa. The expected band size for PKC Iota/PRKCI is at 67 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-gata1a-picoband-antibody-azq90410-boster.html2026-03-17T05:17:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq90410-gata1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish GATA1A Antibody Picoband®Western blot analysis of GATA1A using anti-GATA1A antibody (AZQ90410). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA1A antigen affinity purified polyclonal antibody (AZQ90410) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GATA1A at approximately 45 kDa. The expected band size for GATA1A is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-cyp1a-picoband-antibody-azq8uw07-boster.html2026-03-17T05:17:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq8uw07-cyp1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish CYP1A Antibody Picoband®Western blot analysis of CYP1A using anti-CYP1A antibody (AZQ8UW07). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1A antigen affinity purified polyclonal antibody (AZQ8UW07) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CYP1A at approximately 58 kDa. The expected band size for CYP1A is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-catalase-cat-picoband-antibody-azq9pt92-boster.html2026-03-17T05:17:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/azq9pt92-cat-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish Catalase/CAT Antibody Picoband®Western blot analysis of Catalase/CAT using anti-Catalase/CAT antibody (AZQ9PT92). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates, Lane 4: zebrafish embryo tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Catalase/CAT antigen affinity purified polyclonal antibody (AZQ9PT92) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Catalase/CAT at approximately 59 kDa. The expected band size for Catalase/CAT is at 59 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-mrc1a-picoband-antibody-aza0a0r4inm0-boster.html2026-03-17T05:17:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza0a0r4inm0-mrc1a-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish MRC1A Antibody Picoband®Western blot analysis of MRC1A using anti-MRC1A antibody (AZA0A0R4INM0). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRC1A antigen affinity purified polyclonal antibody (AZA0A0R4INM0) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MRC1A at approximately 165 kDa. The expected band size for MRC1A is at 163 kDa. https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-fancm-picoband-antibody-aza8qlh8-boster.html2026-03-17T05:17:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/z/aza8qlh8-fancm-primary-antibodies-wb-testing-1.jpgAnti-Zebrafish FANCM Antibody Picoband®Western blot analysis of FANCM using anti-FANCM antibody (AZA8QLH8). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates, Lane 2: whole female zebrafish tissue lysates, Lane 3: whole male zebrafish tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FANCM antigen affinity purified polyclonal antibody (AZA8QLH8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FANCM at approximately 232 kDa. The expected band size for FANCM is at 229 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-tubulin-antibody-monoclonal-m05397-2-boster.html2026-03-16T05:11:47+00:00daily0.9 https://www.bosterbio.com/products/human-il-10-picokine-trade-elisa-kit-hsek0416-boster.html2026-03-10T04:43:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0416_3.pngHuman IL-10/Interleukin-10 High Sensitivity ELISA Kit PicoKine®Human IL-10/Interleukin-10 High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/rat-tnf-alpha-picokine-trade-elisa-kit-hsek0526-boster.html2026-03-10T04:43:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0526_1.pngRat TNF Alpha/Tumor necrosis factor High Sensitivity ELISA Kit PicoKine®Rat TNF Alpha/Tumor necrosis factor High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/rat-tnf-alpha-picokine-trade-elisa-kit-hsek0526-boster-1.html2026-03-10T04:43:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0360_1.pngHuman G-CSF High Sensitivity ELISA Kit PicoKine®Human G-CSF High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/mouse-gm-csf-picokine-trade-elisa-kit-hsek0365-boster.html2026-03-10T04:43:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/s/hsek0365_1.pngMouse GM-CSF High Sensitivity ELISA Kit PicoKine®Mouse GM-CSF High Sensitivity PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/mouse-il-28a-b-picokine-elisa-kit-ek2386-boster.html2026-03-10T04:43:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2386.pngMouse IL-28A/B ELISA Kit PicoKine®Mouse IL-28A/B PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/human-pcolce-picokine-elisa-kit-ek2385-boster.html2026-03-10T04:43:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2385.pngHuman Irisin/FNDC5 ELISA Kit PicoKine®Human Irisin/FNDC5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/human-pcolce-picokine-elisa-kit-ek2388-boster.html2026-03-10T04:43:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek2385_1_1.pngHuman Irisin/FNDC5 ELISA Kit PicoKine®Human Irisin/FNDC5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1340022026-03-10T04:43:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0757.pngHuman Fetuin A/AHSG PicoKine® Quick ELISA KitHuman Fetuin A/AHSG PicoKine Quick ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1340032026-03-10T04:43:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0757_1.pngMouse Fractalkine/CX3CL1 PicoKine® Quick ELISA KitHuman Fetuin A/AHSG PicoKine Quick ELISA Kit standard curve https://www.bosterbio.com/catalog/product/view/id/1340042026-03-10T04:43:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/e/fek0733.pngMouse CXCL9/Mig PicoKine® Quick ELISA KitMouse CXCL9/Mig PicoKine Quick ELISA Kit standard curve https://www.bosterbio.com/products/anti-actin-antibody-monoclonal-m02014-6-boster.html2026-03-11T05:02:34+00:00daily0.9 https://www.bosterbio.com/products/anti-afp-antibody-monoclonal-ma00522-5-boster.html2026-03-11T05:02:34+00:00daily0.9 https://www.bosterbio.com/products/anti-bcl-2-antibody-monoclonal-m00040-7-boster.html2026-03-11T05:02:34+00:00daily0.9 https://www.bosterbio.com/products/anti-calcineurin-alpha-antibody-monoclonal-m06469-boster.html2026-03-11T05:02:34+00:00daily0.9 https://www.bosterbio.com/products/anti-caldesmon-smooth-antibody-monoclonal-m04578-3-boster.html2026-03-11T05:02:35+00:00daily0.9 https://www.bosterbio.com/products/anti-calmodulin-antibody-monoclonal-m06609-1-boster.html2026-03-11T05:02:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box_18.pngAnti-Calmodulin Calm1 Antibody (Monoclonal, 35C54)Boster Kit Box https://www.bosterbio.com/products/anti-tyrosine-hydroxylase-antibody-monoclonal-m01917-3-boster.html2026-03-13T05:05:20+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/zebrafish-antibodies/anti-zebrafish-traf7-antibody-dz41770-boster.html2026-03-16T05:11:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340132026-03-16T05:11:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340142026-03-16T05:11:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340152026-03-16T05:11:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340162026-04-06T05:04:10+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340172026-04-06T05:04:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340182026-04-06T05:04:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340192026-03-16T05:11:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340202026-04-06T05:04:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340212026-04-06T05:04:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340222026-04-06T05:04:11+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340232026-03-16T05:11:47+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340242026-03-16T05:11:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340252026-03-16T05:11:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340262026-03-16T05:11:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340272026-03-16T05:11:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340282026-03-16T05:11:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340292026-03-16T05:11:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340302026-03-16T05:11:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340312026-03-16T05:11:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340322026-03-16T05:11:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340332026-03-16T05:11:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340342026-03-16T05:11:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340352026-03-16T05:11:48+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340372026-03-24T05:33:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340382026-03-24T05:33:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340392026-03-24T05:33:57+00:00daily0.9 https://www.bosterbio.com/catalog/product/view/id/1340402026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09810-1-dhrs2-primary-antibodies-wb-testing-1.jpgAnti-DHRS2 Antibody Picoband®Western blot analysis of DHRS2 using anti-DHRS2 antibody (A09810-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHRS2 antigen affinity purified polyclonal antibody (A09810-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHRS2 at approximately 36 kDa. The expected band size for DHRS2 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09810-1-dhrs2-primary-antibodies-if-testing-1.jpgAnti-DHRS2 Antibody Picoband®IF analysis of DHRS2 using anti-DHRS2 antibody (A09810-1) and anti-Alpha Tubulin antibody (M03989-3). DHRS2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DHRS2 Antibody (A09810-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1340412026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08117-1-kdm3b-primary-antibodies-wb-testing-1.jpgAnti-KDM3B Antibody Picoband®Western blot analysis of KDM3B using anti-KDM3B antibody (A08117-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM3B antigen affinity purified polyclonal antibody (A08117-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KDM3B at approximately 210 kDa. The expected band size for KDM3B is at 192 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08117-1-kdm3b-primary-antibodies-ihc-testing-1.jpgAnti-KDM3B Antibody Picoband®IHC analysis of KDM3B using anti-KDM3B antibody (A08117-1). KDM3B was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KDM3B Antibody (A08117-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08117-1-kdm3b-primary-antibodies-if-testing-1.jpgAnti-KDM3B Antibody Picoband®IF analysis of KDM3B using anti-KDM3B antibody (A08117-1) and anti-Alpha Tubulin antibody (M03989-3). KDM3B was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-KDM3B Antibody (A08117-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08117-1-kdm3b-primary-antibodies-fcm-testing-1.jpgAnti-KDM3B Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-KDM3B antibody (A08117-1). Overlay histogram showing HEL cells stained with A08117-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KDM3B Antibody (A08117-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340422026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11772-1-dnaaf2-primary-antibodies-wb-testing-1.jpgAnti-DNAAF2 Antibody Picoband®Western blot analysis of DNAAF2 using anti-DNAAF2 antibody (A11772-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat ovary tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAAF2 antigen affinity purified polyclonal antibody (A11772-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNAAF2 at approximately 91 kDa. The expected band size for DNAAF2 is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11772-1-dnaaf2-primary-antibodies-ihc-testing-1.jpgAnti-DNAAF2 Antibody Picoband®IHC analysis of DNAAF2 using anti-DNAAF2 antibody (A11772-1). DNAAF2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAAF2 Antibody (A11772-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11772-1-dnaaf2-primary-antibodies-if-testing-1.jpgAnti-DNAAF2 Antibody Picoband®IF analysis of DNAAF2 using anti-DNAAF2 antibody (A11772-1) and anti-Alpha Tubulin antibody (M03989-3). DNAAF2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DNAAF2 Antibody (A11772-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1340432026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31774-1-dnaaf4-primary-antibodies-wb-testing-1.jpgAnti-DYX1C1/DNAAF4 Antibody Picoband®Western blot analysis of DYX1C1/DNAAF4 using anti-DYX1C1/DNAAF4 antibody (A31774-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DYX1C1/DNAAF4 antigen affinity purified polyclonal antibody (A31774-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DYX1C1/DNAAF4 at approximately 49 kDa. The expected band size for DYX1C1/DNAAF4 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31774-1-dnaaf4-primary-antibodies-ihc-testing-1.jpgAnti-DYX1C1/DNAAF4 Antibody Picoband®IHC analysis of DYX1C1/DNAAF4 using anti-DYX1C1/DNAAF4 antibody (A31774-1). DYX1C1/DNAAF4 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DYX1C1/DNAAF4 Antibody (A31774-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31774-1-dnaaf4-primary-antibodies-ihc-testing-2.jpgAnti-DYX1C1/DNAAF4 Antibody Picoband®IHC analysis of DYX1C1/DNAAF4 using anti-DYX1C1/DNAAF4 antibody (A31774-1). DYX1C1/DNAAF4 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DYX1C1/DNAAF4 Antibody (A31774-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31774-1-dnaaf4-primary-antibodies-ihc-testing-3.jpgAnti-DYX1C1/DNAAF4 Antibody Picoband®IHC analysis of DYX1C1/DNAAF4 using anti-DYX1C1/DNAAF4 antibody (A31774-1). DYX1C1/DNAAF4 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DYX1C1/DNAAF4 Antibody (A31774-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1340442026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07656-2-dnai1-primary-antibodies-wb-testing-1.jpgAnti-DNAI1 Antibody Picoband®Western blot analysis of DNAI1 using anti-DNAI1 antibody (A07656-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAI1 antigen affinity purified polyclonal antibody (A07656-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNAI1 at approximately 90 kDa. The expected band size for DNAI1 is at 79 kDa. https://www.bosterbio.com/catalog/product/view/id/1340452026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05761-1-dnaja3-primary-antibodies-wb-testing-1.jpgAnti-TID1/DNAJA3 Antibody Picoband®Western blot analysis of TID1/DNAJA3 using anti-TID1/DNAJA3 antibody (A05761-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TID1/DNAJA3 antigen affinity purified polyclonal antibody (A05761-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TID1/DNAJA3 at approximately 40 kDa. The expected band size for TID1/DNAJA3 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05761-1-dnaja3-primary-antibodies-if-testing-1.jpgAnti-TID1/DNAJA3 Antibody Picoband®IF analysis of TID1/DNAJA3 using anti-TID1/DNAJA3 antibody (A05761-1) and anti-Alpha Tubulin antibody (M03989-3). TID1/DNAJA3 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TID1/DNAJA3 Antibody (A05761-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1340462026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10527-1-dnaja4-primary-antibodies-wb-testing-1.jpgAnti-DNAJA4 Antibody Picoband®Western blot analysis of DNAJA4 using anti-DNAJA4 antibody (A10527-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJA4 antigen affinity purified polyclonal antibody (A10527-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNAJA4 at approximately 45 kDa. The expected band size for DNAJA4 is at 45 kDa. https://www.bosterbio.com/catalog/product/view/id/1340472026-04-02T05:01:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10730-2-dnajc9-primary-antibodies-wb-testing-1.jpgAnti-DNAJC9 Antibody Picoband®Western blot analysis of DNAJC9 using anti-DNAJC9 antibody (A10730-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAJC9 antigen affinity purified polyclonal antibody (A10730-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNAJC9 at approximately 35 kDa. The expected band size for DNAJC9 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10730-2-dnajc9-primary-antibodies-ihc-testing-1.jpgAnti-DNAJC9 Antibody Picoband®IHC analysis of DNAJC9 using anti-DNAJC9 antibody (A10730-2). DNAJC9 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNAJC9 Antibody (A10730-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10730-2-dnajc9-primary-antibodies-if-testing-1.jpgAnti-DNAJC9 Antibody Picoband®IF analysis of DNAJC9 using anti-DNAJC9 antibody (A10730-2) and anti-Alpha Tubulin antibody (M03989-3). DNAJC9 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DNAJC9 Antibody (A10730-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10730-2-dnajc9-primary-antibodies-fcm-testing-1.jpgAnti-DNAJC9 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-DNAJC9 antibody (A10730-2). Overlay histogram showing K562 cells stained with A10730-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAJC9 Antibody (A10730-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340482026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12371-3-dnal1-primary-antibodies-wb-testing-1.jpgAnti-DNAL1 Antibody Picoband®Western blot analysis of DNAL1 using anti-DNAL1 antibody (A12371-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNAL1 antigen affinity purified polyclonal antibody (A12371-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNAL1 at approximately 20 kDa. The expected band size for DNAL1 is at 22 kDa. https://www.bosterbio.com/catalog/product/view/id/1340492026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06011-1-dner-primary-antibodies-wb-testing-1.jpgAnti-DNER Antibody Picoband®Western blot analysis of DNER using anti-DNER antibody (A06011-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNER antigen affinity purified polyclonal antibody (A06011-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNER at approximately 130 kDa. The expected band size for DNER is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06011-1-dner-primary-antibodies-ihc-testing-1.jpgAnti-DNER Antibody Picoband®IHC analysis of DNER using anti-DNER antibody (A06011-1). DNER was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNER Antibody (A06011-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06011-1-dner-primary-antibodies-ihc-testing-2.jpgAnti-DNER Antibody Picoband®IHC analysis of DNER using anti-DNER antibody (A06011-1). DNER was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNER Antibody (A06011-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06011-1-dner-primary-antibodies-ihc-testing-3.jpgAnti-DNER Antibody Picoband®IHC analysis of DNER using anti-DNER antibody (A06011-1). DNER was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNER Antibody (A06011-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06011-1-dner-primary-antibodies-fcm-testing-1.jpgAnti-DNER Antibody Picoband®Flow Cytometry analysis of Hela cells using anti-DNER antibody (A06011-1). Overlay histogram showing Hela cells stained with A06011-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DNER Antibody (A06011-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340502026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07679-1-dtd1-primary-antibodies-wb-testing-1.jpgAnti-DTD1 Antibody Picoband®Western blot analysis of DTD1 using anti-DTD1 antibody (A07679-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DTD1 antigen affinity purified polyclonal antibody (A07679-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DTD1 at approximately 24 kDa. The expected band size for DTD1 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07679-1-dtd1-primary-antibodies-if-testing-1.jpgAnti-DTD1 Antibody Picoband®IF analysis of DTD1 using anti-DTD1 antibody (A07679-1). DTD1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DTD1 Antibody (A07679-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1340512026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05610-1-dtna-primary-antibodies-wb-testing-1.jpgAnti-DTNA Antibody Picoband®Western blot analysis of DTNA using anti-DTNA antibody (A05610-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DTNA antigen affinity purified polyclonal antibody (A05610-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DTNA at approximately 84 kDa. The expected band size for DTNA is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05610-1-dtna-primary-antibodies-if-testing-1.jpgAnti-DTNA Antibody Picoband®IF analysis of DTNA using anti-DTNA antibody (A05610-1). DTNA was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DTNA Antibody (A05610-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1340522026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07348-1-dtx3l-primary-antibodies-wb-testing-1.jpgAnti-DTX3L Antibody Picoband®Western blot analysis of DTX3L using anti-DTX3L antibody (A07348-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DTX3L antigen affinity purified polyclonal antibody (A07348-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DTX3L at approximately 84 kDa. The expected band size for DTX3L is at 84 kDa. https://www.bosterbio.com/catalog/product/view/id/1340532026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11243-dusp18-primary-antibodies-wb-testing-1.jpgAnti-DUSP18 Antibody Picoband®Western blot analysis of DUSP18 using anti-DUSP18 antibody (A11243). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP18 antigen affinity purified polyclonal antibody (A11243) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DUSP18 at approximately 21 kDa. The expected band size for DUSP18 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11243-dusp18-primary-antibodies-if-testing-1.jpgAnti-DUSP18 Antibody Picoband®IF analysis of DUSP18 using anti-DUSP18 antibody (A11243) and anti-Alpha Tubulin antibody (M03989-3). DUSP18 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DUSP18 Antibody (A11243) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1340542026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09732-dusp23-primary-antibodies-wb-testing-1.jpgAnti-DUSP23 Antibody Picoband®Western blot analysis of DUSP23 using anti-DUSP23 antibody (A09732). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP23 antigen affinity purified polyclonal antibody (A09732) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DUSP23 at approximately 15 kDa. The expected band size for DUSP23 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09732-dusp23-primary-antibodies-ihc-testing-1.jpgAnti-DUSP23 Antibody Picoband®IHC analysis of DUSP23 using anti-DUSP23 antibody (A09732). DUSP23 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DUSP23 Antibody (A09732) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09732-dusp23-primary-antibodies-if-testing-1.jpgAnti-DUSP23 Antibody Picoband®IF analysis of DUSP23 using anti-DUSP23 antibody (A09732) and anti-Alpha Tubulin antibody (M03989-3). DUSP23 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DUSP23 Antibody (A09732) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09732-dusp23-primary-antibodies-ip-testing-1.jpgAnti-DUSP23 Antibody Picoband®Immunoprecipitating DUSP23 in HEL whole cell lysate. Western blot analysis of DUSP23 using anti-DUSP23 antibody (A09732). Lane 1: HEL whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DUSP23 antibody in HEL whole cell lysate, Lane 3: anti-DUSP23 antibody (2μg) + HEL whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DUSP23 antigen affinity purified polyclonal antibody (A09732) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DUSP23 at approximately 15 kDa. The expected band size for DUSP23 is at 17 kDa. https://www.bosterbio.com/catalog/product/view/id/1340552026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17132-1-duxa-primary-antibodies-wb-testing-1.jpgAnti-DUXA Antibody Picoband®Western blot analysis of DUXA using anti-DUXA antibody (A17132-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUXA antigen affinity purified polyclonal antibody (A17132-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DUXA at approximately 28 kDa. The expected band size for DUXA is at 24 kDa. https://www.bosterbio.com/catalog/product/view/id/1340562026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04782-2-dyrk3-primary-antibodies-wb-testing-1.jpgAnti-DYRK3 Antibody Picoband®Western blot analysis of DYRK3 using anti-DYRK3 antibody (A04782-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates。After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DYRK3 antigen affinity purified polyclonal antibody (A04782-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DYRK3 at approximately 66 kDa. The expected band size for DYRK3 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04782-2-dyrk3-primary-antibodies-if-testing-1.jpgAnti-DYRK3 Antibody Picoband®IF analysis of DYRK3 using anti-DYRK3 antibody (A04782-2) and anti-Alpha Tubulin antibody (M03989-3). DYRK3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DYRK3 Antibody (A04782-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1340572026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00282-3-ebf1-2-3-4-primary-antibodies-wb-testing-1.jpgAnti-EBF1/2/3/4 Antibody Picoband®Western blot analysis of EBF1/2/3/4 using anti-EBF1/2/3/4 antibody (A00282-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBF1/2/3/4 antigen affinity purified polyclonal antibody (A00282-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EBF1/2/3/4 at approximately 70 kDa. The expected band size for EBF1/2/3/4 is at 64 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00282-3-ebf1-2-3-4-primary-antibodies-ihc-testing-1.jpgAnti-EBF1/2/3/4 Antibody Picoband®IHC analysis of EBF1/2/3/4 using anti-EBF1/2/3/4 antibody (A00282-3). EBF1/2/3/4 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EBF1/2/3/4 Antibody (A00282-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1340582026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09966-1-ebna1bp2-primary-antibodies-wb-testing-1.jpgAnti-EBP2/EBNA1BP2 Antibody Picoband®Western blot analysis of EBP2/EBNA1BP2 using anti-EBP2/EBNA1BP2 antibody (A09966-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBP2/EBNA1BP2 antigen affinity purified polyclonal antibody (A09966-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EBP2/EBNA1BP2 at approximately 39 kDa. The expected band size for EBP2/EBNA1BP2 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09966-1-ebna1bp2-primary-antibodies-ihc-testing-1.jpgAnti-EBP2/EBNA1BP2 Antibody Picoband®IHC analysis of EBP2/EBNA1BP2 using anti-EBP2/EBNA1BP2 antibody (A09966-1). EBP2/EBNA1BP2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EBP2/EBNA1BP2 Antibody (A09966-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09966-1-ebna1bp2-primary-antibodies-ihc-testing-2.jpgAnti-EBP2/EBNA1BP2 Antibody Picoband®IHC analysis of EBP2/EBNA1BP2 using anti-EBP2/EBNA1BP2 antibody (A09966-1). EBP2/EBNA1BP2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EBP2/EBNA1BP2 Antibody (A09966-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09966-1-ebna1bp2-primary-antibodies-if-testing-1.jpgAnti-EBP2/EBNA1BP2 Antibody Picoband®IF analysis of EBP2/EBNA1BP2 using anti-EBP2/EBNA1BP2 antibody (A09966-1) and anti-Alpha Tubulin antibody (M03989-3). EBP2/EBNA1BP2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EBP2/EBNA1BP2 Antibody (A09966-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09966-1-ebna1bp2-primary-antibodies-ip-testing-1.jpgAnti-EBP2/EBNA1BP2 Antibody Picoband®Immunoprecipitating EBP2/EBNA1BP2 in A549 whole cell lysate. Western blot analysis of EBP2/EBNA1BP2 using anti-EBP2/EBNA1BP2 antibody (A09966-1). Lane 1: A549 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-EBP2/EBNA1BP2 antibody in A549 whole cell lysate, Lane 3: anti-EBP2/EBNA1BP2 antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EBP2/EBNA1BP2 antigen affinity purified polyclonal antibody (A09966-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EBP2/EBNA1BP2 at approximately 39 kDa. The expected band size for EBP2/EBNA1BP2 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09966-1-ebna1bp2-primary-antibodies-fcm-testing-1.jpgAnti-EBP2/EBNA1BP2 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-EBP2/EBNA1BP2 antibody (A09966-1). Overlay histogram showing A549 cells stained with A09966-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EBP2/EBNA1BP2 Antibody (A09966-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340592026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07818-1-ech1-primary-antibodies-wb-testing-1.jpgAnti-ECH1 Antibody Picoband®Western blot analysis of ECH1 using anti-ECH1 antibody (A07818-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECH1 antigen affinity purified polyclonal antibody (A07818-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ECH1 at approximately 30 kDa. The expected band size for ECH1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07818-1-ech1-primary-antibodies-ihc-testing-1.jpgAnti-ECH1 Antibody Picoband®IHC analysis of ECH1 using anti-ECH1 antibody (A07818-1). ECH1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECH1 Antibody (A07818-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07818-1-ech1-primary-antibodies-ihc-testing-2.jpgAnti-ECH1 Antibody Picoband®IHC analysis of ECH1 using anti-ECH1 antibody (A07818-1). ECH1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECH1 Antibody (A07818-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07818-1-ech1-primary-antibodies-ihc-testing-3.jpgAnti-ECH1 Antibody Picoband®IHC analysis of ECH1 using anti-ECH1 antibody (A07818-1). ECH1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECH1 Antibody (A07818-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07818-1-ech1-primary-antibodies-ihc-testing-4.jpgAnti-ECH1 Antibody Picoband®IHC analysis of ECH1 using anti-ECH1 antibody (A07818-1). ECH1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECH1 Antibody (A07818-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07818-1-ech1-primary-antibodies-if-testing-1.jpgAnti-ECH1 Antibody Picoband®IF analysis of ECH1 using anti-ECH1 antibody (A07818-1) and anti-Alpha Tubulin antibody (M03989-3). ECH1 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ECH1 Antibody (A07818-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1340602026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17328-1-echdc2-primary-antibodies-wb-testing-1.jpgAnti-ECHDC2 Antibody Picoband®Western blot analysis of ECHDC2 using anti-ECHDC2 antibody (A17328-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECHDC2 antigen affinity purified polyclonal antibody (A17328-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ECHDC2 at approximately 29 kDa. The expected band size for ECHDC2 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17328-1-echdc2-primary-antibodies-ihc-testing-1.jpgAnti-ECHDC2 Antibody Picoband®IHC analysis of ECHDC2 using anti-ECHDC2 antibody (A17328-1). ECHDC2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECHDC2 Antibody (A17328-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17328-1-echdc2-primary-antibodies-ihc-testing-2.jpgAnti-ECHDC2 Antibody Picoband®IHC analysis of ECHDC2 using anti-ECHDC2 antibody (A17328-1). ECHDC2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECHDC2 Antibody (A17328-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1340612026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14149-1-echdc3-primary-antibodies-wb-testing-1.jpgAnti-ECHDC3 Antibody Picoband®Western blot analysis of ECHDC3 using anti-ECHDC3 antibody (A14149-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECHDC3 antigen affinity purified polyclonal antibody (A14149-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ECHDC3 at approximately 33 kDa. The expected band size for ECHDC3 is at 33 kDa. https://www.bosterbio.com/catalog/product/view/id/1340622026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09257-1-ecm2-primary-antibodies-wb-testing-1.jpgAnti-ECM2 Antibody Picoband®Western blot analysis of ECM2 using anti-ECM2 antibody (A09257-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECM2 antigen affinity purified polyclonal antibody (A09257-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ECM2 at approximately 80 kDa. The expected band size for ECM2 is at 80 kDa. https://www.bosterbio.com/catalog/product/view/id/1340632026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08532-4-eef1e1-primary-antibodies-wb-testing-1.jpgAnti-EEF1E1 Antibody Picoband®Western blot analysis of EEF1E1 using anti-EEF1E1 antibody (A08532-4). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEF1E1 antigen affinity purified polyclonal antibody (A08532-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EEF1E1 at approximately 16 kDa. The expected band size for EEF1E1 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08532-4-eef1e1-primary-antibodies-ihc-testing-1.jpgAnti-EEF1E1 Antibody Picoband®IHC analysis of EEF1E1 using anti-EEF1E1 antibody (A08532-4). EEF1E1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EEF1E1 Antibody (A08532-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08532-4-eef1e1-primary-antibodies-ihc-testing-2.jpgAnti-EEF1E1 Antibody Picoband®IHC analysis of EEF1E1 using anti-EEF1E1 antibody (A08532-4). EEF1E1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EEF1E1 Antibody (A08532-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1340642026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05955-1-efhc1-primary-antibodies-wb-testing-1.jpgAnti-EFHC1 Antibody Picoband®Western blot analysis of EFHC1 using anti-EFHC1 antibody (A05955-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EFHC1 antigen affinity purified polyclonal antibody (A05955-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EFHC1 at approximately 74 kDa. The expected band size for EFHC1 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05955-1-efhc1-primary-antibodies-ihc-testing-1.jpgAnti-EFHC1 Antibody Picoband®IHC analysis of EFHC1 using anti-EFHC1 antibody (A05955-1). EFHC1 was detected in a paraffin-embedded section of human fallopian tube tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EFHC1 Antibody (A05955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05955-1-efhc1-primary-antibodies-ihc-testing-2.jpgAnti-EFHC1 Antibody Picoband®IHC analysis of EFHC1 using anti-EFHC1 antibody (A05955-1). EFHC1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EFHC1 Antibody (A05955-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1340652026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15489-ttll8-primary-antibodies-wb-testing-1.jpgAnti-TTLL8 Antibody Picoband®Western blot analysis of TTLL8 using anti-TTLL8 antibody (A15489). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL8 antigen affinity purified polyclonal antibody (A15489) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TTLL8 at approximately 95 kDa. The expected band size for TTLL8 is at 95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15489-ttll8-primary-antibodies-fcm-testing-1.jpgAnti-TTLL8 Antibody Picoband®Flow Cytometry analysis of Hela cells using anti-TTLL8 antibody (A15489). Overlay histogram showing Hela cells stained with A15489 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TTLL8 Antibody (A15489, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340662026-04-02T05:01:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12038-eid3-primary-antibodies-wb-testing-1.jpgAnti-EID3 Antibody Picoband®Western blot analysis of EID3 using anti-EID3 antibody (A12038). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EID3 antigen affinity purified polyclonal antibody (A12038) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EID3 at approximately 38 kDa. The expected band size for EID3 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12038-eid3-primary-antibodies-if-testing-1.jpgAnti-EID3 Antibody Picoband®IF analysis of EID3 using anti-EID3 antibody (A12038) and anti-Alpha Tubulin antibody (M03989-3). EID3 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EID3 Antibody (A12038) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12038-eid3-primary-antibodies-fcm-testing-1.jpgAnti-EID3 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-EID3 antibody (A12038). Overlay histogram showing K562 cells stained with A12038 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EID3 Antibody (A12038, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340672026-04-02T05:01:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07789-3-eif3k-primary-antibodies-wb-testing-1.jpgAnti-EIF3K Antibody Picoband®Western blot analysis of EIF3K using anti-EIF3K antibody (A07789-3). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3K antigen affinity purified polyclonal antibody (A07789-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EIF3K at approximately 25 kDa. The expected band size for EIF3K is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07789-3-eif3k-primary-antibodies-ihc-testing-1.jpgAnti-EIF3K Antibody Picoband®IHC analysis of EIF3K using anti-EIF3K antibody (A07789-3). EIF3K was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EIF3K Antibody (A07789-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07789-3-eif3k-primary-antibodies-if-testing-1.jpgAnti-EIF3K Antibody Picoband®IF analysis of EIF3K using anti-EIF3K antibody (A07789-3). EIF3K was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EIF3K Antibody (A07789-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07789-3-eif3k-primary-antibodies-ip-testing-1.jpgAnti-EIF3K Antibody Picoband®Immunoprecipitating EIF3K in Hela whole cell lysate. Western blot analysis of EIF3K using anti-EIF3K antibody (A07789-3). Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-EIF3K antibody in Hela whole cell lysate, Lane 3: anti-EIF3K antibody (2μg) + Hela whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EIF3K antigen affinity purified polyclonal antibody (A07789-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EIF3K at approximately 25 kDa. The expected band size for EIF3K is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07789-3-eif3k-primary-antibodies-fcm-testing-1.jpgAnti-EIF3K Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-EIF3K antibody (A07789-3). Overlay histogram showing RT4 cells stained with A07789-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EIF3K Antibody (A07789-3, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340682026-04-02T05:01:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06563-dnase1l3-primary-antibodies-wb-testing-1.jpgAnti-DNASE1L3 Antibody Picoband®Western blot analysis of DNASE1L3 using anti-DNASE1L3 antibody (A06563). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNASE1L3 antigen affinity purified polyclonal antibody (A06563) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNASE1L3 at approximately 36 kDa. The expected band size for DNASE1L3 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06563-dnase1l3-primary-antibodies-ihc-testing-1.jpgAnti-DNASE1L3 Antibody Picoband®IHC analysis of DNASE1L3 using anti-DNASE1L3 antibody (A06563). DNASE1L3 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DNASE1L3 Antibody (A06563) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06563-dnase1l3-primary-antibodies-if-testing-1.jpgAnti-DNASE1L3 Antibody Picoband®IF analysis of DNASE1L3 using anti-DNASE1L3 antibody (A06563) and anti-Alpha Tubulin antibody (M03989-3). DNASE1L3 was detected in an immunocytochemical section of HaCat cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DNASE1L3 Antibody (A06563) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1340692026-04-02T05:01:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13042-2-elac1-primary-antibodies-wb-testing-1.jpgAnti-ELAC1 Antibody Picoband®Western blot analysis of ELAC1 using anti-ELAC1 antibody (A13042-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human REH whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELAC1 antigen affinity purified polyclonal antibody (A13042-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ELAC1 at approximately 48 kDa. The expected band size for ELAC1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13042-2-elac1-primary-antibodies-ihc-testing-1.jpgAnti-ELAC1 Antibody Picoband®IHC analysis of ELAC1 using anti-ELAC1 antibody (A13042-2). ELAC1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ELAC1 Antibody (A13042-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13042-2-elac1-primary-antibodies-fcm-testing-1.jpgAnti-ELAC1 Antibody Picoband®Flow Cytometry analysis of HEL cells using anti-ELAC1 antibody (A13042-2). Overlay histogram showing HEL cells stained with A13042-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ELAC1 Antibody (A13042-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340702026-04-02T05:01:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08879-emilin2-primary-antibodies-wb-testing-1.jpgAnti-EMILIN2 Antibody Picoband®Western blot analysis of EMILIN2 using anti-EMILIN2 antibody (A08879). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EMILIN2 antigen affinity purified polyclonal antibody (A08879) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EMILIN2 at approximately 125 kDa. The expected band size for EMILIN2 is at 116 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08879-emilin2-primary-antibodies-ihc-testing-1.jpgAnti-EMILIN2 Antibody Picoband®IHC analysis of EMILIN2 using anti-EMILIN2 antibody (A08879). EMILIN2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EMILIN2 Antibody (A08879) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08879-emilin2-primary-antibodies-ihc-testing-2.jpgAnti-EMILIN2 Antibody Picoband®IHC analysis of EMILIN2 using anti-EMILIN2 antibody (A08879). EMILIN2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EMILIN2 Antibody (A08879) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1340712026-04-02T05:01:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04477-1-emsy-primary-antibodies-wb-testing-1.jpgAnti-C11orf30/EMSY Antibody Picoband®Western blot analysis of C11orf30/EMSY using anti-C11orf30/EMSY antibody (A04477-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C11orf30/EMSY antigen affinity purified polyclonal antibody (A04477-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for C11orf30/EMSY at approximately 160 kDa. The expected band size for C11orf30/EMSY is at 141 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04477-1-emsy-primary-antibodies-ihc-testing-1.jpgAnti-C11orf30/EMSY Antibody Picoband®IHC analysis of C11orf30/EMSY using anti-C11orf30/EMSY antibody (A04477-1). C11orf30/EMSY was detected in a paraffin-embedded section of human prostatic hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-C11orf30/EMSY Antibody (A04477-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04477-1-emsy-primary-antibodies-if-testing-1.jpgAnti-C11orf30/EMSY Antibody Picoband®IF analysis of C11orf30/EMSY using anti-C11orf30/EMSY antibody (A04477-1) and anti-Alpha Tubulin antibody (M03989-3). C11orf30/EMSY was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-C11orf30/EMSY Antibody (A04477-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04477-1-emsy-primary-antibodies-fcm-testing-1.jpgAnti-C11orf30/EMSY Antibody Picoband®Flow Cytometry analysis of THP-1 cells using anti-C11orf30/EMSY antibody (A04477-1). Overlay histogram showing THP-1 cells stained with A04477-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C11orf30/EMSY Antibody (A04477-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340722026-04-02T05:01:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14829-1-endod1-primary-antibodies-wb-testing-1.jpgAnti-ENDOD1 Antibody Picoband®Western blot analysis of ENDOD1 using anti-ENDOD1 antibody (A14829-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENDOD1 antigen affinity purified polyclonal antibody (A14829-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ENDOD1 at approximately 55 kDa. The expected band size for ENDOD1 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14829-1-endod1-primary-antibodies-ihc-testing-1.jpgAnti-ENDOD1 Antibody Picoband®IHC analysis of ENDOD1 using anti-ENDOD1 antibody (A14829-1). ENDOD1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENDOD1 Antibody (A14829-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14829-1-endod1-primary-antibodies-ihc-testing-2.jpgAnti-ENDOD1 Antibody Picoband®IHC analysis of ENDOD1 using anti-ENDOD1 antibody (A14829-1). ENDOD1 was detected in a paraffin-embedded section of human prostatic hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENDOD1 Antibody (A14829-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14829-1-endod1-primary-antibodies-if-testing-1.jpgAnti-ENDOD1 Antibody Picoband®IF analysis of ENDOD1 using anti-ENDOD1 antibody (A14829-1). ENDOD1 was detected in an immunocytochemical section of HaCat cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ENDOD1 Antibody (A14829-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14829-1-endod1-primary-antibodies-fcm-testing-1.jpgAnti-ENDOD1 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-ENDOD1 antibody (A14829-1). Overlay histogram showing Jurkat cells stained with A14829-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ENDOD1 Antibody (A14829-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340732026-04-02T05:01:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08768-1-engase-primary-antibodies-wb-testing-1.jpgAnti-ENGASE Antibody Picoband®Western blot analysis of ENGASE using anti-ENGASE antibody (A08768-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human REH whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENGASE antigen affinity purified polyclonal antibody (A08768-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ENGASE at approximately 84 kDa. The expected band size for ENGASE is at 84 kDa. https://www.bosterbio.com/catalog/product/view/id/1340742026-04-02T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07325-2-enosf1-primary-antibodies-wb-testing-1.jpgAnti-ENOSF1 Antibody Picoband®Western blot analysis of ENOSF1 using anti-ENOSF1 antibody (A07325-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENOSF1 antigen affinity purified polyclonal antibody (A07325-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ENOSF1 at approximately 50 kDa. The expected band size for ENOSF1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07325-2-enosf1-primary-antibodies-ihc-testing-1.jpgAnti-ENOSF1 Antibody Picoband®IHC analysis of ENOSF1 using anti-ENOSF1 antibody (A07325-2). ENOSF1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ENOSF1 Antibody (A07325-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07325-2-enosf1-primary-antibodies-ip-testing-1.jpgAnti-ENOSF1 Antibody Picoband®Immunoprecipitating ENOSF1 in RT4 whole cell lysate. Western blot analysis of ENOSF1 using anti-ENOSF1 antibody (A07325-2). Lane 1: RT4 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-ENOSF1 antibody in RT4 whole cell lysate, Lane 3: anti-ENOSF1 antibody (2μg) + RT4 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ENOSF1 antigen affinity purified polyclonal antibody (A07325-2) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ENOSF1 at approximately 50 kDa. The expected band size for ENOSF1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07325-2-enosf1-primary-antibodies-fcm-testing-1.jpgAnti-ENOSF1 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-ENOSF1 antibody (A07325-2). Overlay histogram showing K562 cells stained with A07325-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ENOSF1 Antibody (A07325-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340752026-04-02T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11099-enpp6-primary-antibodies-wb-testing-1.jpgAnti-ENPP6 Antibody Picoband®Western blot analysis of ENPP6 using anti-ENPP6 antibody (A11099). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENPP6 antigen affinity purified polyclonal antibody (A11099) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ENPP6 at approximately 50 kDa. The expected band size for ENPP6 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11099-enpp6-primary-antibodies-fcm-testing-1.jpgAnti-ENPP6 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-ENPP6 antibody (A11099). Overlay histogram showing MCF-7 cells stained with A11099 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ENPP6 Antibody (A11099, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340762026-04-02T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11381-2-enpp7-primary-antibodies-wb-testing-1.jpgAnti-ENPP7 Antibody Picoband®Western blot analysis of ENPP7 using anti-ENPP7 antibody (A11381-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human REH whole cell lysates, Lane 5: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENPP7 antigen affinity purified polyclonal antibody (A11381-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ENPP7 at approximately 60 kDa. The expected band size for ENPP7 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11381-2-enpp7-primary-antibodies-fcm-testing-1.jpgAnti-ENPP7 Antibody Picoband®Flow Cytometry analysis of CACO-2 cells using anti-ENPP7 antibody (A11381-2). Overlay histogram showing CACO-2 cells stained with A11381-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ENPP7 Antibody (A11381-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340772026-04-02T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11721-1-epc2-primary-antibodies-wb-testing-1.jpgAnti-EPC2 Antibody Picoband®Western blot analysis of EPC2 using anti-EPC2 antibody (A11721-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPC2 antigen affinity purified polyclonal antibody (A11721-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPC2 at approximately 91 kDa. The expected band size for EPC2 is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11721-1-epc2-primary-antibodies-ihc-testing-1.jpgAnti-EPC2 Antibody Picoband®IHC analysis of EPC2 using anti-EPC2 antibody (A11721-1). EPC2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPC2 Antibody (A11721-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11721-1-epc2-primary-antibodies-if-testing-1.jpgAnti-EPC2 Antibody Picoband®IF analysis of EPC2 using anti-EPC2 antibody (A11721-1) and anti-Alpha Tubulin antibody (M03989-3). EPC2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EPC2 Antibody (A11721-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11721-1-epc2-primary-antibodies-fcm-testing-1.jpgAnti-EPC2 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-EPC2 antibody (A11721-1). Overlay histogram showing RT4 cells stained with A11721-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EPC2 Antibody (A11721-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340782026-04-02T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12195-1-epdr1-primary-antibodies-wb-testing-1.jpgAnti-EPDR1 Antibody Picoband®Western blot analysis of EPDR1 using anti-EPDR1 antibody (A12195-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPDR1 antigen affinity purified polyclonal antibody (A12195-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPDR1 at approximately 27 kDa. The expected band size for EPDR1 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12195-1-epdr1-primary-antibodies-ihc-testing-1.jpgAnti-EPDR1 Antibody Picoband®IHC analysis of EPDR1 using anti-EPDR1 antibody (A12195-1). EPDR1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPDR1 Antibody (A12195-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12195-1-epdr1-primary-antibodies-ihc-testing-2.jpgAnti-EPDR1 Antibody Picoband®IHC analysis of EPDR1 using anti-EPDR1 antibody (A12195-1). EPDR1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPDR1 Antibody (A12195-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12195-1-epdr1-primary-antibodies-ihc-testing-3.jpgAnti-EPDR1 Antibody Picoband®IHC analysis of EPDR1 using anti-EPDR1 antibody (A12195-1). EPDR1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPDR1 Antibody (A12195-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12195-1-epdr1-primary-antibodies-ihc-testing-4.jpgAnti-EPDR1 Antibody Picoband®IHC analysis of EPDR1 using anti-EPDR1 antibody (A12195-1). EPDR1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPDR1 Antibody (A12195-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12195-1-epdr1-primary-antibodies-ihc-testing-5.jpgAnti-EPDR1 Antibody Picoband®IHC analysis of EPDR1 using anti-EPDR1 antibody (A12195-1). EPDR1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPDR1 Antibody (A12195-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12195-1-epdr1-primary-antibodies-ihc-testing-6.jpgAnti-EPDR1 Antibody Picoband®IHC analysis of EPDR1 using anti-EPDR1 antibody (A12195-1). EPDR1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPDR1 Antibody (A12195-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1340792026-04-02T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03171-ank2-primary-antibodies-wb-testing-1.jpgAnti-ANK2 Antibody Picoband®Western blot analysis of ANK2 using anti-ANK2 antibody (A03171). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANK2 antigen affinity purified polyclonal antibody (A03171) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ANK2 at approximately 260 kDa. The expected band size for ANK2 is at 434 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03171-ank2-primary-antibodies-fcm-testing-1.jpgAnti-ANK2 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-ANK2 antibody (A03171). Overlay histogram showing SH-SY5Y cells stained with A03171 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ANK2 Antibody (A03171, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340802026-04-02T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08759-1-ermap-primary-antibodies-wb-testing-1.jpgAnti-ERMAP Antibody Picoband®Western blot analysis of ERMAP using anti-ERMAP antibody (A08759-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERMAP antigen affinity purified polyclonal antibody (A08759-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERMAP at approximately 60 kDa. The expected band size for ERMAP is at 53 kDa. https://www.bosterbio.com/catalog/product/view/id/1340812026-04-02T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08004-1-esrp2-primary-antibodies-wb-testing-1.jpgAnti-ESRP2 Antibody Picoband®Western blot analysis of ESRP2 using anti-ESRP2 antibody (A08004-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESRP2 antigen affinity purified polyclonal antibody (A08004-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ESRP2 at approximately 78 kDa. The expected band size for ESRP2 is at 78 kDa. https://www.bosterbio.com/catalog/product/view/id/1340822026-04-02T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07472-1-dmtf1-primary-antibodies-wb-testing-1.jpgAnti-DMTF1 Antibody Picoband®Western blot analysis of DMTF1 using anti-DMTF1 antibody (A07472-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DMTF1 antigen affinity purified polyclonal antibody (A07472-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DMTF1 at approximately 90 kDa. The expected band size for DMTF1 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07472-1-dmtf1-primary-antibodies-ihc-testing-1.jpgAnti-DMTF1 Antibody Picoband®IHC analysis of DMTF1 using anti-DMTF1 antibody (A07472-1). DMTF1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DMTF1 Antibody (A07472-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07472-1-dmtf1-primary-antibodies-ihc-testing-2.jpgAnti-DMTF1 Antibody Picoband®IHC analysis of DMTF1 using anti-DMTF1 antibody (A07472-1). DMTF1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DMTF1 Antibody (A07472-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07472-1-dmtf1-primary-antibodies-ihc-testing-3.jpgAnti-DMTF1 Antibody Picoband®IHC analysis of DMTF1 using anti-DMTF1 antibody (A07472-1). DMTF1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DMTF1 Antibody (A07472-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1340832026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09715-nit1-primary-antibodies-wb-testing-1.jpgAnti-NIT1 Antibody Picoband®Western blot analysis of NIT1 using anti-NIT1 antibody (A09715). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NIT1 antigen affinity purified polyclonal antibody (A09715) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NIT1 at approximately 32 kDa. The expected band size for NIT1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09715-nit1-primary-antibodies-ihc-testing-1.jpgAnti-NIT1 Antibody Picoband®IHC analysis of NIT1 using anti-NIT1 antibody (A09715). NIT1 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NIT1 Antibody (A09715) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09715-nit1-primary-antibodies-ihc-testing-2.jpgAnti-NIT1 Antibody Picoband®IHC analysis of NIT1 using anti-NIT1 antibody (A09715). NIT1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NIT1 Antibody (A09715) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1340842026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06028-2-sprtn-primary-antibodies-wb-testing-1.jpgAnti-Spartan/SPRTN Antibody Picoband®Western blot analysis of Spartan/SPRTN using anti-Spartan/SPRTN antibody (A06028-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spartan/SPRTN antigen affinity purified polyclonal antibody (A06028-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Spartan/SPRTN at approximately 65 kDa. The expected band size for Spartan/SPRTN is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06028-2-sprtn-primary-antibodies-ihc-testing-1.jpgAnti-Spartan/SPRTN Antibody Picoband®IHC analysis of Spartan/SPRTN using anti-Spartan/SPRTN antibody (A06028-2). Spartan/SPRTN was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Spartan/SPRTN Antibody (A06028-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06028-2-sprtn-primary-antibodies-ihc-testing-2.jpgAnti-Spartan/SPRTN Antibody Picoband®IHC analysis of Spartan/SPRTN using anti-Spartan/SPRTN antibody (A06028-2). Spartan/SPRTN was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Spartan/SPRTN Antibody (A06028-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06028-2-sprtn-primary-antibodies-ihc-testing-3.jpgAnti-Spartan/SPRTN Antibody Picoband®IHC analysis of Spartan/SPRTN using anti-Spartan/SPRTN antibody (A06028-2). Spartan/SPRTN was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Spartan/SPRTN Antibody (A06028-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1340852026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16058-1-fabp9-primary-antibodies-wb-testing-1.jpgAnti-FABP9 Antibody Picoband®Western blot analysis of FABP9 using anti-FABP9 antibody (A16058-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human OE19 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP9 antigen affinity purified polyclonal antibody (A16058-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FABP9 at approximately 15 kDa. The expected band size for FABP9 is at 15 kDa. https://www.bosterbio.com/catalog/product/view/id/1340862026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07124-2-efhd2-primary-antibodies-wb-testing-1.jpgAnti-EFHD2 Antibody Picoband®Western blot analysis of EFHD2 using anti-EFHD2 antibody (A07124-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EFHD2 antigen affinity purified polyclonal antibody (A07124-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EFHD2 at approximately 27 kDa. The expected band size for EFHD2 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07124-2-efhd2-primary-antibodies-ihc-testing-1.jpgAnti-EFHD2 Antibody Picoband®IHC analysis of EFHD2 using anti-EFHD2 antibody (A07124-2). EFHD2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EFHD2 Antibody (A07124-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07124-2-efhd2-primary-antibodies-ihc-testing-2.jpgAnti-EFHD2 Antibody Picoband®IHC analysis of EFHD2 using anti-EFHD2 antibody (A07124-2). EFHD2 was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EFHD2 Antibody (A07124-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07124-2-efhd2-primary-antibodies-ihc-testing-3.jpgAnti-EFHD2 Antibody Picoband®IHC analysis of EFHD2 using anti-EFHD2 antibody (A07124-2). EFHD2 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EFHD2 Antibody (A07124-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07124-2-efhd2-primary-antibodies-ip-testing-1.jpgAnti-EFHD2 Antibody Picoband®Immunoprecipitating EFHD2 in A549 whole cell lysate. Western blot analysis of EFHD2 using anti-EFHD2 antibody (A07124-2). Lane 1: A549 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-EFHD2 antibody in A549 whole cell lysate, Lane 3: anti-EFHD2 antibody (2μg) + A549 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-EFHD2 antigen affinity purified polyclonal antibody (A07124-2) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for EFHD2 at approximately 27 kDa. The expected band size for EFHD2 is at 27 kDa. https://www.bosterbio.com/catalog/product/view/id/1340872026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12937-3-epyc-primary-antibodies-wb-testing-1.jpgAnti-EPYC Antibody Picoband®Western blot analysis of EPYC using anti-EPYC antibody (A12937-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPYC antigen affinity purified polyclonal antibody (A12937-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPYC at approximately 37 kDa. The expected band size for EPYC is at 37 kDa. https://www.bosterbio.com/catalog/product/view/id/1340882026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01383-3-etv1-primary-antibodies-wb-testing-1.jpgAnti-ETV1 Antibody Picoband®Western blot analysis of ETV1 using anti-ETV1 antibody (A01383-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETV1 antigen affinity purified polyclonal antibody (A01383-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ETV1 at approximately 45 kDa. The expected band size for ETV1 is at 55 kDa. https://www.bosterbio.com/catalog/product/view/id/1340892026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08148-1-epb42-primary-antibodies-wb-testing-1.jpgAnti-EPB42 Antibody Picoband®Western blot analysis of EPB42 using anti-EPB42 antibody (A08148-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPB42 antigen affinity purified polyclonal antibody (A08148-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPB42 at approximately 77 kDa. The expected band size for EPB42 is at 77 kDa. https://www.bosterbio.com/catalog/product/view/id/1340902026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12387-2-eps8l1-primary-antibodies-wb-testing-1.jpgAnti-EPS8L1 Antibody Picoband®Western blot analysis of EPS8L1 using anti-EPS8L1 antibody (A12387-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCT116 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPS8L1 antigen affinity purified polyclonal antibody (A12387-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPS8L1 at approximately 85 kDa. The expected band size for EPS8L1 is at 80 kDa. https://www.bosterbio.com/catalog/product/view/id/1340912026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11370-1-eral1-primary-antibodies-wb-testing-1.jpgAnti-ERAL1 Antibody Picoband®Western blot analysis of ERAL1 using anti-ERAL1 antibody (A11370-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERAL1 antigen affinity purified polyclonal antibody (A11370-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERAL1 at approximately 40 kDa. The expected band size for ERAL1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11370-1-eral1-primary-antibodies-ihc-testing-1.jpgAnti-ERAL1 Antibody Picoband®IHC analysis of ERAL1 using anti-ERAL1 antibody (A11370-1). ERAL1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ERAL1 Antibody (A11370-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11370-1-eral1-primary-antibodies-ip-testing-1.jpgAnti-ERAL1 Antibody Picoband®Immunoprecipitating ERAL1 in K562 whole cell lysate. Western blot analysis of ERAL1 using anti-ERAL1 antibody (A11370-1). Lane 1: K562 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-ERAL1 antibody in K562 whole cell lysate, Lane 3: anti-ERAL1 antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ERAL1 antigen affinity purified polyclonal antibody (A11370-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ERAL1 at approximately 40 kDa. The expected band size for ERAL1 is at 48 kDa. https://www.bosterbio.com/catalog/product/view/id/1340922026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31721-ess2-primary-antibodies-wb-testing-1.jpgAnti-DGCR14/ESS2 Antibody Picoband®Western blot analysis of DGCR14/ESS2 using anti-DGCR14/ESS2 antibody (A31721). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human LNCAP whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DGCR14/ESS2 antigen affinity purified polyclonal antibody (A31721) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DGCR14/ESS2 at approximately 60 kDa. The expected band size for DGCR14/ESS2 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31721-ess2-primary-antibodies-ihc-testing-1.jpgAnti-DGCR14/ESS2 Antibody Picoband®IHC analysis of DGCR14/ESS2 using anti-DGCR14/ESS2 antibody (A31721). DGCR14/ESS2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DGCR14/ESS2 Antibody (A31721) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31721-ess2-primary-antibodies-if-testing-1.jpgAnti-DGCR14/ESS2 Antibody Picoband®IF analysis of DGCR14/ESS2 using anti-DGCR14/ESS2 antibody (A31721) and anti-Alpha Tubulin antibody (M03989-3). DGCR14/ESS2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DGCR14/ESS2 Antibody (A31721) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a31721-ess2-primary-antibodies-ip-testing-1.jpgAnti-DGCR14/ESS2 Antibody Picoband®Immunoprecipitating DGCR14/ESS2 in K562 whole cell lysate. Western blot analysis of DGCR14/ESS2 using anti-DGCR14/ESS2 antibody (A31721). Lane 1: K562 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DGCR14/ESS2 antibody in K562 whole cell lysate, Lane 3: anti-DGCR14/ESS2 antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DGCR14/ESS2 antigen affinity purified polyclonal antibody (A31721) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DGCR14/ESS2 at approximately 60 kDa. The expected band size for DGCR14/ESS2 is at 53 kDa. https://www.bosterbio.com/catalog/product/view/id/1340932026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05557-1-ethe1-primary-antibodies-wb-testing-1.jpgAnti-ETHE1 Antibody Picoband®Western blot analysis of ETHE1 using anti-ETHE1 antibody (A05557-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETHE1 antigen affinity purified polyclonal antibody (A05557-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ETHE1 at approximately 28 kDa. The expected band size for ETHE1 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05557-1-ethe1-primary-antibodies-ihc-testing-1.jpgAnti-ETHE1 Antibody Picoband®IHC analysis of ETHE1 using anti-ETHE1 antibody (A05557-1). ETHE1 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ETHE1 Antibody (A05557-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05557-1-ethe1-primary-antibodies-ihc-testing-2.jpgAnti-ETHE1 Antibody Picoband®IHC analysis of ETHE1 using anti-ETHE1 antibody (A05557-1). ETHE1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ETHE1 Antibody (A05557-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05557-1-ethe1-primary-antibodies-ihc-testing-3.jpgAnti-ETHE1 Antibody Picoband®IHC analysis of ETHE1 using anti-ETHE1 antibody (A05557-1). ETHE1 was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ETHE1 Antibody (A05557-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05557-1-ethe1-primary-antibodies-ihc-testing-4.jpgAnti-ETHE1 Antibody Picoband®IHC analysis of ETHE1 using anti-ETHE1 antibody (A05557-1). ETHE1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ETHE1 Antibody (A05557-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05557-1-ethe1-primary-antibodies-if-testing-1.jpgAnti-ETHE1 Antibody Picoband®IF analysis of ETHE1 using anti-ETHE1 antibody (A05557-1). ETHE1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ETHE1 Antibody (A05557-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05557-1-ethe1-primary-antibodies-ip-testing-1.jpgAnti-ETHE1 Antibody Picoband®Immunoprecipitating ETHE1 in RT4 whole cell lysate. Western blot analysis of ETHE1 using anti-ETHE1 antibody (A05557-1). Lane 1: RT4 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-ETHE1 antibody in RT4 whole cell lysate, Lane 3: anti-ETHE1 antibody (2μg) + RT4 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ETHE1 antigen affinity purified polyclonal antibody (A05557-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ETHE1 at approximately 28 kDa. The expected band size for ETHE1 is at 28 kDa. https://www.bosterbio.com/catalog/product/view/id/1340942026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10379-etnk1-primary-antibodies-wb-testing-1.jpgAnti-ETNK1 Antibody Picoband®Western blot analysis of ETNK1 using anti-ETNK1 antibody (A10379). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETNK1 antigen affinity purified polyclonal antibody (A10379) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ETNK1 at approximately 51 kDa. The expected band size for ETNK1 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10379-etnk1-primary-antibodies-ihc-testing-1.jpgAnti-ETNK1 Antibody Picoband®IHC analysis of ETNK1 using anti-ETNK1 antibody (A10379). ETNK1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ETNK1 Antibody (A10379) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10379-etnk1-primary-antibodies-if-testing-1.jpgAnti-ETNK1 Antibody Picoband®IF analysis of ETNK1 using anti-ETNK1 antibody (A10379). ETNK1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ETNK1 Antibody (A10379) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10379-etnk1-primary-antibodies-fcm-testing-1.jpgAnti-ETNK1 Antibody Picoband®Flow Cytometry analysis of A549 cells using anti-ETNK1 antibody (A10379). Overlay histogram showing A549 cells stained with A10379 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ETNK1 Antibody (A10379, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10379-etnk1-primary-antibodies-fcm-testing-2.jpgAnti-ETNK1 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-ETNK1 antibody (A10379). Overlay histogram showing MCF-7 cells stained with A10379 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ETNK1 Antibody (A10379, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1340952026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12947-2-etnk2-primary-antibodies-wb-testing-1.jpgAnti-ETNK2 Antibody Picoband®Western blot analysis of ETNK2 using anti-ETNK2 antibody (A12947-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETNK2 antigen affinity purified polyclonal antibody (A12947-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ETNK2 at approximately 45 kDa. The expected band size for ETNK2 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12947-2-etnk2-primary-antibodies-if-testing-1.jpgAnti-ETNK2 Antibody Picoband®IF analysis of ETNK2 using anti-ETNK2 antibody (A12947-2). ETNK2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ETNK2 Antibody (A12947-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1340962026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06185-1-evi5-primary-antibodies-wb-testing-1.jpgAnti-EVI5 Antibody Picoband®Western blot analysis of EVI5 using anti-EVI5 antibody (A06185-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EVI5 antigen affinity purified polyclonal antibody (A06185-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EVI5 at approximately 93 kDa. The expected band size for EVI5 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06185-1-evi5-primary-antibodies-ihc-testing-1.jpgAnti-EVI5 Antibody Picoband®IHC analysis of EVI5 using anti-EVI5 antibody (A06185-1). EVI5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EVI5 Antibody (A06185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1340972026-04-02T05:01:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09421-1-evpl-primary-antibodies-wb-testing-1.jpgAnti-Envoplakin/EVPL Antibody Picoband®Western blot analysis of Envoplakin/EVPL using anti-Envoplakin/EVPL antibody (A09421-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Hacat whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Envoplakin/EVPL antigen affinity purified polyclonal antibody (A09421-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Envoplakin/EVPL at approximately 250 kDa. The expected band size for Envoplakin/EVPL is at 232 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09421-1-evpl-primary-antibodies-ihc-testing-1.jpgAnti-Envoplakin/EVPL Antibody Picoband®IHC analysis of Envoplakin/EVPL using anti-Envoplakin/EVPL antibody (A09421-1). Envoplakin/EVPL was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Envoplakin/EVPL Antibody (A09421-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09421-1-evpl-primary-antibodies-ihc-testing-2.jpgAnti-Envoplakin/EVPL Antibody Picoband®IHC analysis of Envoplakin/EVPL using anti-Envoplakin/EVPL antibody (A09421-1). Envoplakin/EVPL was detected in a paraffin-embedded section of human skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Envoplakin/EVPL Antibody (A09421-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09421-1-evpl-primary-antibodies-ihc-testing-3.jpgAnti-Envoplakin/EVPL Antibody Picoband®IHC analysis of Envoplakin/EVPL using anti-Envoplakin/EVPL antibody (A09421-1). Envoplakin/EVPL was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Envoplakin/EVPL Antibody (A09421-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09421-1-evpl-primary-antibodies-if-testing-1.jpgAnti-Envoplakin/EVPL Antibody Picoband®IF analysis of Envoplakin/EVPL using anti-Envoplakin/EVPL antibody (A09421-1). Envoplakin/EVPL was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Envoplakin/EVPL Antibody (A09421-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1340982026-04-02T05:01:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10155-2-exog-primary-antibodies-wb-testing-1.jpgAnti-EXOG Antibody Picoband®Western blot analysis of EXOG using anti-EXOG antibody (A10155-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EXOG antigen affinity purified polyclonal antibody (A10155-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EXOG at approximately 41 kDa. The expected band size for EXOG is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10155-2-exog-primary-antibodies-ihc-testing-1.jpgAnti-EXOG Antibody Picoband®IHC analysis of EXOG using anti-EXOG antibody (A10155-2). EXOG was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EXOG Antibody (A10155-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10155-2-exog-primary-antibodies-if-testing-1.jpgAnti-EXOG Antibody Picoband®IF analysis of EXOG using anti-EXOG antibody (A10155-2) and anti-Alpha Tubulin antibody (M03989-3). EXOG was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EXOG Antibody (A10155-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1340992026-04-02T05:01:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02065-2-ext2-primary-antibodies-wb-testing-1.jpgAnti-EXT2 Antibody Picoband®Western blot analysis of EXT2 using anti-EXT2 antibody (A02065-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EXT2 antigen affinity purified polyclonal antibody (A02065-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EXT2 at approximately 75 kDa. The expected band size for EXT2 is at 82 kDa. https://www.bosterbio.com/catalog/product/view/id/1341002026-04-02T05:01:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10534-1-fam162a-primary-antibodies-wb-testing-1.jpgAnti-FAM162A Antibody Picoband®Western blot analysis of FAM162A using anti-FAM162A antibody (A10534-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM162A antigen affinity purified polyclonal antibody (A10534-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FAM162A at approximately 17 kDa. The expected band size for FAM162A is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10534-1-fam162a-primary-antibodies-ihc-testing-1.jpgAnti-FAM162A Antibody Picoband®IHC analysis of FAM162A using anti-FAM162A antibody (A10534-1). FAM162A was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAM162A Antibody (A10534-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10534-1-fam162a-primary-antibodies-ihc-testing-2.jpgAnti-FAM162A Antibody Picoband®IHC analysis of FAM162A using anti-FAM162A antibody (A10534-1). FAM162A was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAM162A Antibody (A10534-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10534-1-fam162a-primary-antibodies-if-testing-1.jpgAnti-FAM162A Antibody Picoband®IF analysis of FAM162A using anti-FAM162A antibody (A10534-1). FAM162A was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FAM162A Antibody (A10534-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10534-1-fam162a-primary-antibodies-ip-testing-1.jpgAnti-FAM162A Antibody Picoband®Immunoprecipitating FAM162A in RT4 whole cell lysate. Western blot analysis of FAM162A using anti-FAM162A antibody (A10534-1). Lane 1: RT4 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-FAM162A antibody in RT4 whole cell lysate, Lane 3: anti-FAM162A antibody (2μg) + RT4 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FAM162A antigen affinity purified polyclonal antibody (A10534-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FAM162A at approximately 17 kDa. The expected band size for FAM162A is at 17 kDa. https://www.bosterbio.com/catalog/product/view/id/1341012026-04-02T05:01:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12412-1-fate1-primary-antibodies-wb-testing-1.jpgAnti-FATE1 Antibody Picoband®Western blot analysis of FATE1 using anti-FATE1 antibody (A12412-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FATE1 antigen affinity purified polyclonal antibody (A12412-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FATE1 at approximately 24 kDa. The expected band size for FATE1 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12412-1-fate1-primary-antibodies-ihc-testing-1.jpgAnti-FATE1 Antibody Picoband®IHC analysis of FATE1 using anti-FATE1 antibody (A12412-1). FATE1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FATE1 Antibody (A12412-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1341022026-04-02T05:01:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14171-dancr-primary-antibodies-wb-testing-1.jpgAnti-DANCR Antibody Picoband®Western blot analysis of DANCR using anti-DANCR antibody (A14171). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DANCR antigen affinity purified polyclonal antibody (A14171) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DANCR at approximately 22 kDa. The expected band size for DANCR is at 22 kDa. https://www.bosterbio.com/catalog/product/view/id/1341032026-04-02T05:01:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11920-2-efhd1-primary-antibodies-wb-testing-1.jpgAnti-EFHD1 Antibody Picoband®Western blot analysis of EFHD1 using anti-EFHD1 antibody (A11920-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EFHD1 antigen affinity purified polyclonal antibody (A11920-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EFHD1 at approximately 27 kDa. The expected band size for EFHD1 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11920-2-efhd1-primary-antibodies-ihc-testing-1.jpgAnti-EFHD1 Antibody Picoband®IHC analysis of EFHD1 using anti-EFHD1 antibody (A11920-2). EFHD1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EFHD1 Antibody (A11920-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11920-2-efhd1-primary-antibodies-ihc-testing-2.jpgAnti-EFHD1 Antibody Picoband®IHC analysis of EFHD1 using anti-EFHD1 antibody (A11920-2). EFHD1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EFHD1 Antibody (A11920-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11920-2-efhd1-primary-antibodies-if-testing-1.jpgAnti-EFHD1 Antibody Picoband®IF analysis of EFHD1 using anti-EFHD1 antibody (A11920-2). EFHD1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EFHD1 Antibody (A11920-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1341042026-04-02T05:01:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13192-1-tppp3-primary-antibodies-wb-testing-1.jpgAnti-TPPP3 Antibody Picoband®Western blot analysis of TPPP3 using anti-TPPP3 antibody (A13192-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPPP3 antigen affinity purified polyclonal antibody (A13192-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TPPP3 at approximately 19 kDa. The expected band size for TPPP3 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13192-1-tppp3-primary-antibodies-ihc-testing-1.jpgAnti-TPPP3 Antibody Picoband®IHC analysis of TPPP3 using anti-TPPP3 antibody (A13192-1). TPPP3 was detected in a paraffin-embedded section of human fallopian tube tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TPPP3 Antibody (A13192-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1341052026-04-02T05:01:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12186-1-tm4sf4-primary-antibodies-wb-testing-1.jpgAnti-TM4SF4 Antibody Picoband®Western blot analysis of TM4SF4 using anti-TM4SF4 antibody (A12186-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TM4SF4 antigen affinity purified polyclonal antibody (A12186-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TM4SF4 at approximately 21 kDa. The expected band size for TM4SF4 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12186-1-tm4sf4-primary-antibodies-ihc-testing-1.jpgAnti-TM4SF4 Antibody Picoband®IHC analysis of TM4SF4 using anti-TM4SF4 antibody (A12186-1). TM4SF4 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TM4SF4 Antibody (A12186-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1341062026-04-02T05:01:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04611-1-mt-nd3-primary-antibodies-wb-testing-1.jpgAnti-ND3/MT-ND3 Antibody Picoband®Western blot analysis of ND3/MT-ND3 using anti-ND3/MT-ND3 antibody (A04611-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ND3/MT-ND3 antigen affinity purified polyclonal antibody (A04611-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ND3/MT-ND3 at approximately 13 kDa. The expected band size for ND3/MT-ND3 is at 13 kDa. https://www.bosterbio.com/catalog/product/view/id/1341072026-04-02T05:01:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04611-2-mt-nd3-primary-antibodies-wb-testing-1.jpgAnti-ND3/MT-ND3 Antibody Picoband®Western blot analysis of ND3/MT-ND3 using anti-ND3/MT-ND3 antibody (A04611-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse stomach tissue lysates, Lane 3: mouse small intestine tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ND3/MT-ND3 antigen affinity purified polyclonal antibody (A04611-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ND3/MT-ND3 at approximately 15 kDa. The expected band size for ND3/MT-ND3 is at 13 kDa. https://www.bosterbio.com/catalog/product/view/id/1341092026-03-30T05:00:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1628.pngHuman NT-proBNP/NPPB ELISA Kit PicoKine®Human NT-proBNP/NPPB ELISA Kit PicoKine standard curve https://www.bosterbio.com/catalog/product/view/id/1341102026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14587-1-dexi-primary-antibodies-ihc-testing-1.jpgAnti-DEXI AntibodyIHC analysis of DEXI using anti-DEXI antibody (A14587-1). DEXI was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DEXI Antibody (A14587-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14587-1-dexi-primary-antibodies-ihc-testing-2.jpgAnti-DEXI AntibodyIHC analysis of DEXI using anti-DEXI antibody (A14587-1). DEXI was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DEXI Antibody (A14587-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14587-1-dexi-primary-antibodies-ihc-testing-3.jpgAnti-DEXI AntibodyIHC analysis of DEXI using anti-DEXI antibody (A14587-1). DEXI was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DEXI Antibody (A14587-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1341112026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02728-1-dnase1-primary-antibodies-wb-testing-1.jpgAnti-DNase I/DNASE1 Antibody Picoband®Western blot analysis of DNase I/DNASE1 using anti-DNase I/DNASE1 antibody (A02728-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNase I/DNASE1 antigen affinity purified polyclonal antibody (A02728-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNase I/DNASE1 at approximately 42 kDa. The expected band size for DNase I/DNASE1 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02728-1-dnase1-primary-antibodies-fcm-testing-1.jpgAnti-DNase I/DNASE1 Antibody Picoband®Flow Cytometry analysis of CACO-2 cells using anti-DNase I/DNASE1 antibody (A02728-1). Overlay histogram showing CACO-2 cells stained with A02728-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-DNase I/DNASE1 Antibody (A02728-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341122026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01108-2-trh-primary-antibodies-wb-testing-1.jpgAnti-Trh Antibody Picoband®Western blot analysis of Trh using anti-Trh antibody (A01108-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, , Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Trh antigen affinity purified polyclonal antibody (A01108-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Trh at approximately 30 kDa. The expected band size for Trh is at 27 kDa. https://www.bosterbio.com/catalog/product/view/id/1341132026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a33371-1-mt-atp8-primary-antibodies-wb-testing-1.jpgAnti-ATP8/MT-ATP8 Antibody Picoband®Western blot analysis of ATP8/MT-ATP8 using anti-ATP8/MT-ATP8 antibody (A33371-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse HT22 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP8/MT-ATP8 antigen affinity purified polyclonal antibody (A33371-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATP8/MT-ATP8 at approximately 8 kDa. The expected band size for ATP8/MT-ATP8 is at 8 kDa. https://www.bosterbio.com/catalog/product/view/id/1341142026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13392-2-mxra8-primary-antibodies-wb-testing-1.jpgAnti-MXRA8 Antibody Picoband®Western blot analysis of MXRA8 using anti-MXRA8 antibody (A13392-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: rat ovary tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: rat SHZ-88 whole cell lysates, Lane 6: mouse 4T1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MXRA8 antigen affinity purified polyclonal antibody (A13392-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MXRA8 at approximately 70 kDa. The expected band size for MXRA8 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13392-2-mxra8-primary-antibodies-wb-testing-2.jpgAnti-MXRA8 Antibody Picoband®Western blot analysis of MXRA8 using anti-MXRA8 antibody (A13392-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat ovary tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat SHZ-88 whole cell lysates, Lane 4: mouse ovary tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MXRA8 antigen affinity purified polyclonal antibody (A13392-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MXRA8 at approximately 55-60 kDa. The expected band size for MXRA8 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13392-2-mxra8-primary-antibodies-if-testing-1.jpgAnti-MXRA8 Antibody Picoband®IF analysis of MXRA8 using anti-MXRA8 antibody (A13392-2) and anti-Alpha Tubulin antibody (M03989-3). MXRA8 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MXRA8 Antibody (A13392-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13392-2-mxra8-primary-antibodies-fcm-testing-1.jpgAnti-MXRA8 Antibody Picoband®Flow Cytometry analysis of U2OS cells using anti-MXRA8 antibody (A13392-2). Overlay histogram showing U2OS cells stained with A13392-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MXRA8 Antibody (A13392-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13392-2-mxra8-primary-antibodies-fcm-testing-2.jpgAnti-MXRA8 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-MXRA8 antibody (A13392-2). Overlay histogram showing K562 cells stained with A13392-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MXRA8 Antibody (A13392-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341152026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07853-1-mycl-primary-antibodies-wb-testing-1.jpgAnti-L-Myc/MYCL Antibody Picoband®Western blot analysis of L-Myc/MYCL using anti-L-Myc/MYCL antibody (A07853-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U266 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-L-Myc/MYCL antigen affinity purified polyclonal antibody (A07853-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for L-Myc/MYCL at approximately 62 kDa. The expected band size for L-Myc/MYCL is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07853-1-mycl-primary-antibodies-wb-testing-2.jpgAnti-L-Myc/MYCL Antibody Picoband®Western blot analysis of L-Myc/MYCL using anti-L-Myc/MYCL antibody (A07853-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-L-Myc/MYCL antigen affinity purified polyclonal antibody (A07853-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for L-Myc/MYCL at approximately 62 kDa. The expected band size for L-Myc/MYCL is at 40 kDa. https://www.bosterbio.com/catalog/product/view/id/1341162026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07978-2-nanos2-primary-antibodies-wb-testing-1.jpgAnti-NANOS2 Antibody Picoband®Western blot analysis of NANOS2 using anti-NANOS2 antibody (A07978-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NANOS2 antigen affinity purified polyclonal antibody (A07978-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NANOS2 at approximately 16 kDa. The expected band size for NANOS2 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07978-2-nanos2-primary-antibodies-ihc-testing-1.jpgAnti-NANOS2 Antibody Picoband®IHC analysis of NANOS2 using anti-NANOS2 antibody (A07978-2). NANOS2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NANOS2 Antibody (A07978-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1341172026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12015-dnali1-primary-antibodies-wb-testing-1.jpgAnti-DNALI1 Antibody Picoband®Western blot analysis of DNALI1 using anti-DNALI1 antibody (A12015). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNALI1 antigen affinity purified polyclonal antibody (A12015) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DNALI1 at approximately 30 kDa. The expected band size for DNALI1 is at 30 kDa. https://www.bosterbio.com/catalog/product/view/id/1341182026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07713-2-atg101-primary-antibodies-wb-testing-1.jpgAnti-ATG101 Antibody Picoband®Western blot analysis of ATG101 using anti-ATG101 antibody (A07713-2). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse 3T3-L1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG101 antigen affinity purified polyclonal antibody (A07713-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATG101 at approximately 35 kDa. The expected band size for ATG101 is at 25 kDa. https://www.bosterbio.com/catalog/product/view/id/1341192026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14276-1-aar2-primary-antibodies-wb-testing-1.jpgAnti-C20orf4/AAR2 Antibody Picoband®Western blot analysis of C20orf4/AAR2 using anti-C20orf4/AAR2 antibody (A14276-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C20orf4/AAR2 antigen affinity purified polyclonal antibody (A14276-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for C20orf4/AAR2 at approximately 43 kDa. The expected band size for C20orf4/AAR2 is at 43 kDa. https://www.bosterbio.com/catalog/product/view/id/1341202026-04-03T05:00:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10794-1-evx1-primary-antibodies-wb-testing-1.jpgAnti-EVX1 Antibody Picoband®Western blot analysis of EVX1 using anti-EVX1 antibody (A10794-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EVX1 antigen affinity purified polyclonal antibody (A10794-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EVX1 at approximately 45 kDa. The expected band size for EVX1 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10794-1-evx1-primary-antibodies-if-testing-1.jpgAnti-EVX1 Antibody Picoband®IF analysis of EVX1 using anti-EVX1 antibody (A10794-1) and anti-Alpha Tubulin antibody (M03989-3). EVX1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EVX1 Antibody (A10794-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10794-1-evx1-primary-antibodies-fcm-testing-1.jpgAnti-EVX1 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-EVX1 antibody (A10794-1). Overlay histogram showing Jurkat cells stained with A10794-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EVX1 Antibody (A10794-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341212026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08953-1-exosc4-primary-antibodies-wb-testing-1.jpgAnti-EXOSC4 Antibody Picoband®Western blot analysis of EXOSC4 using anti-EXOSC4 antibody (A08953-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HCT116 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EXOSC4 antigen affinity purified polyclonal antibody (A08953-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EXOSC4 at approximately 26 kDa. The expected band size for EXOSC4 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08953-1-exosc4-primary-antibodies-fcm-testing-1.jpgAnti-EXOSC4 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-EXOSC4 antibody (A08953-1). Overlay histogram showing 293T cells stained with A08953-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EXOSC4 Antibody (A08953-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341222026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12862-extl1-primary-antibodies-wb-testing-1.jpgAnti-EXTL1 Antibody Picoband®Western blot analysis of EXTL1 using anti-EXTL1 antibody (A12862). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EXTL1 antigen affinity purified polyclonal antibody (A12862) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EXTL1 at approximately 75 kDa. The expected band size for EXTL1 is at 75 kDa. https://www.bosterbio.com/catalog/product/view/id/1341232026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08784-2-f2rl2-primary-antibodies-wb-testing-1.jpgAnti-F2RL2 Antibody Picoband®Western blot analysis of F2RL2 using anti-F2RL2 antibody (A08784-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-F2RL2 antigen affinity purified polyclonal antibody (A08784-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for F2RL2 at approximately 50 kDa. The expected band size for F2RL2 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08784-2-f2rl2-primary-antibodies-if-testing-1.jpgAnti-F2RL2 Antibody Picoband®IF analysis of F2RL2 using anti-F2RL2 antibody (A08784-2). F2RL2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-F2RL2 Antibody (A08784-2) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1341242026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00537-1-f9-primary-antibodies-wb-testing-1.jpgAnti-Factor IX/PTC/F9 Antibody Picoband®Western blot analysis of Factor IX/PTC/F9 using anti-Factor IX/PTC/F9 antibody (A00537-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor IX/PTC/F9 antigen affinity purified polyclonal antibody (A00537-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Factor IX/PTC/F9 at approximately 52 kDa. The expected band size for Factor IX/PTC/F9 is at 52 kDa. https://www.bosterbio.com/catalog/product/view/id/1341252026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02047-1-f13a1-primary-antibodies-wb-testing-1.jpgAnti-Factor XIIIa/F13A1 Antibody Picoband®Western blot analysis of Factor XIIIa/F13A1 using anti-Factor XIIIa/F13A1 antibody (A02047-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor XIIIa/F13A1 antigen affinity purified polyclonal antibody (A02047-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Factor XIIIa/F13A1 at approximately 85 kDa. The expected band size for Factor XIIIa/F13A1 is at 83 kDa. https://www.bosterbio.com/catalog/product/view/id/1341262026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11158-dchs2-primary-antibodies-wb-testing-1.jpgAnti-DCHS2 Antibody Picoband®Western blot analysis of DCHS2 using anti-DCHS2 antibody (A11158). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: rat UMR-106 whole cell lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DCHS2 antigen affinity purified polyclonal antibody (A11158) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DCHS2 at approximately 90 kDa. The expected band size for DCHS2 is at 370 kDa. https://www.bosterbio.com/catalog/product/view/id/1341272026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12277-1-ddi2-primary-antibodies-wb-testing-1.jpgAnti-DDI2 Antibody Picoband®Western blot analysis of DDI2 using anti-DDI2 antibody (A12277-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDI2 antigen affinity purified polyclonal antibody (A12277-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DDI2 at approximately 50 kDa. The expected band size for DDI2 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12277-1-ddi2-primary-antibodies-if-testing-1.jpgAnti-DDI2 Antibody Picoband®IF analysis of DDI2 using anti-DDI2 antibody (A12277-1) and anti-Alpha Tubulin antibody (M03989-3). DDI2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DDI2 Antibody (A12277-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12277-1-ddi2-primary-antibodies-ip-testing-1.jpgAnti-DDI2 Antibody Picoband®Immunoprecipitating DDI2 in Jurkat whole cell lysate. Western blot analysis of DDI2 using anti-DDI2 antibody (A12277-1). Lane 1: Jurkat whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-DDI2 antibody in Jurkat whole cell lysate, Lane 3: anti-DDI2 antibody (2μg) + Jurkat whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DDI2 antigen affinity purified polyclonal antibody (A12277-1) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for DDI2 at approximately 50 kDa. The expected band size for DDI2 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12277-1-ddi2-primary-antibodies-fcm-testing-1.jpgAnti-DDI2 Antibody Picoband®Flow Cytometry analysis of 293T cells using anti-DDI2 antibody (A12277-1). Overlay histogram showing 293T cells stained with A12277-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDI2 Antibody (A12277-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341282026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07378-3-diaph2-primary-antibodies-wb-testing-1.jpgAnti-DIAPH2 Antibody Picoband®Western blot analysis of DIAPH2 using anti-DIAPH2 antibody (A07378-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCT116 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIAPH2 antigen affinity purified polyclonal antibody (A07378-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DIAPH2 at approximately 125 kDa. The expected band size for DIAPH2 is at 126 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07378-3-diaph2-primary-antibodies-if-testing-1.jpgAnti-DIAPH2 Antibody Picoband®IF analysis of DIAPH2 using anti-DIAPH2 antibody (A07378-3). DIAPH2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DIAPH2 Antibody (A07378-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1341292026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10010-fam20b-primary-antibodies-wb-testing-1.jpgAnti-FAM20B Antibody Picoband®Western blot analysis of FAM20B using anti-FAM20B antibody (A10010). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM20B antigen affinity purified polyclonal antibody (A10010) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FAM20B at approximately 46 kDa. The expected band size for FAM20B is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10010-fam20b-primary-antibodies-ihc-testing-1.jpgAnti-FAM20B Antibody Picoband®IHC analysis of FAM20B using anti-FAM20B antibody (A10010). FAM20B was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAM20B Antibody (A10010) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10010-fam20b-primary-antibodies-ihc-testing-2.jpgAnti-FAM20B Antibody Picoband®IHC analysis of FAM20B using anti-FAM20B antibody (A10010). FAM20B was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAM20B Antibody (A10010) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10010-fam20b-primary-antibodies-ihc-testing-3.jpgAnti-FAM20B Antibody Picoband®IHC analysis of FAM20B using anti-FAM20B antibody (A10010). FAM20B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAM20B Antibody (A10010) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10010-fam20b-primary-antibodies-ihc-testing-4.jpgAnti-FAM20B Antibody Picoband®IHC analysis of FAM20B using anti-FAM20B antibody (A10010). FAM20B was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAM20B Antibody (A10010) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1341302026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08800-1-fam83d-primary-antibodies-wb-testing-1.jpgAnti-FAM83D Antibody Picoband®Western blot analysis of FAM83D using anti-FAM83D antibody (A08800-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM83D antigen affinity purified polyclonal antibody (A08800-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FAM83D at approximately 64 kDa. The expected band size for FAM83D is at 64 kDa. https://www.bosterbio.com/catalog/product/view/id/1341312026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17465-1-fam83f-primary-antibodies-wb-testing-1.jpgAnti-FAM83F Antibody Picoband®Western blot analysis of FAM83F using anti-FAM83F antibody (A17465-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM83F antigen affinity purified polyclonal antibody (A17465-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FAM83F at approximately 60 kDa. The expected band size for FAM83F is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17465-1-fam83f-primary-antibodies-ihc-testing-1.jpgAnti-FAM83F Antibody Picoband®IHC analysis of FAM83F using anti-FAM83F antibody (A17465-1). FAM83F was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAM83F Antibody (A17465-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17465-1-fam83f-primary-antibodies-ihc-testing-2.jpgAnti-FAM83F Antibody Picoband®IHC analysis of FAM83F using anti-FAM83F antibody (A17465-1). FAM83F was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAM83F Antibody (A17465-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a17465-1-fam83f-primary-antibodies-fcm-testing-1.jpgAnti-FAM83F Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-FAM83F antibody (A17465-1). Overlay histogram showing RT4 cells stained with A17465-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-FAM83F Antibody (A17465-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341322026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05758-1-fam83h-primary-antibodies-wb-testing-1.jpgAnti-FAM83H Antibody Picoband®Western blot analysis of FAM83H using anti-FAM83H antibody (A05758-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM83H antigen affinity purified polyclonal antibody (A05758-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FAM83H at approximately 150 kDa. The expected band size for FAM83H is at 127 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05758-1-fam83h-primary-antibodies-ihc-testing-1.jpgAnti-FAM83H Antibody Picoband®IHC analysis of FAM83H using anti-FAM83H antibody (A05758-1). FAM83H was detected in a paraffin-embedded section of human esophagus cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAM83H Antibody (A05758-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05758-1-fam83h-primary-antibodies-if-testing-1.jpgAnti-FAM83H Antibody Picoband®IF analysis of FAM83H using anti-FAM83H antibody (A05758-1). FAM83H was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FAM83H Antibody (A05758-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1341332026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08753-fam111a-primary-antibodies-wb-testing-1.jpgAnti-FAM111A Antibody Picoband®Western blot analysis of FAM111A using anti-FAM111A antibody (A08753). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM111A antigen affinity purified polyclonal antibody (A08753) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FAM111A at approximately 70 kDa. The expected band size for FAM111A is at 70 kDa. https://www.bosterbio.com/catalog/product/view/id/1341342026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07791-3-niban1-primary-antibodies-wb-testing-1.jpgAnti-FAM129A/NIBAN1 Antibody Picoband®Western blot analysis of FAM129A/NIBAN1 using anti-FAM129A/NIBAN1 antibody (A07791-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM129A/NIBAN1 antigen affinity purified polyclonal antibody (A07791-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FAM129A/NIBAN1 at approximately 150 kDa. The expected band size for FAM129A/NIBAN1 is at 103 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07791-3-niban1-primary-antibodies-ihc-testing-1.jpgAnti-FAM129A/NIBAN1 Antibody Picoband®IHC analysis of FAM129A/NIBAN1 using anti-FAM129A/NIBAN1 antibody (A07791-3). FAM129A/NIBAN1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAM129A/NIBAN1 Antibody (A07791-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07791-3-niban1-primary-antibodies-ip-testing-1.jpgAnti-FAM129A/NIBAN1 Antibody Picoband®Immunoprecipitating FAM129A/NIBAN1 in K562 whole cell lysate. Western blot analysis of FAM129A/NIBAN1 using anti-FAM129A/NIBAN1 antibody (A07791-3). Lane 1: K562 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-FAM129A/NIBAN1 antibody in K562 whole cell lysate, Lane 3: anti-FAM129A/NIBAN1 antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FAM129A/NIBAN1 antigen affinity purified polyclonal antibody (A07791-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FAM129A/NIBAN1 at approximately 150 kDa. The expected band size for FAM129A/NIBAN1 is at 103 kDa. https://www.bosterbio.com/catalog/product/view/id/1341352026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08050-1-fblim1-primary-antibodies-wb-testing-1.jpgAnti-FBLIM1 Antibody Picoband®Western blot analysis of FBLIM1 using anti-FBLIM1 antibody (A08050-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBLIM1 antigen affinity purified polyclonal antibody (A08050-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBLIM1 at approximately 48 kDa. The expected band size for FBLIM1 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08050-1-fblim1-primary-antibodies-fcm-testing-1.jpgAnti-FBLIM1 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-FBLIM1 antibody (A08050-1). Overlay histogram showing U251 cells stained with A08050-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FBLIM1 Antibody (A08050-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341362026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16837-1-fbxl6-primary-antibodies-wb-testing-1.jpgAnti-FBXL6 Antibody Picoband®Western blot analysis of FBXL6 using anti-FBXL6 antibody (A16837-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXL6 antigen affinity purified polyclonal antibody (A16837-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXL6 at approximately 70 kDa. The expected band size for FBXL6 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16837-1-fbxl6-primary-antibodies-if-testing-1.jpgAnti-FBXL6 Antibody Picoband®IF analysis of FBXL6 using anti-FBXL6 antibody (A16837-1) and anti-Alpha Tubulin antibody (M03989-3). FBXL6 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FBXL6 Antibody (A16837-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1341372026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14936-1-fbxl18-primary-antibodies-wb-testing-1.jpgAnti-FBXL18 Antibody Picoband®Western blot analysis of FBXL18 using anti-FBXL18 antibody (A14936-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse ovary tissue lysates, Lane 8: mouse ID8 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXL18 antigen affinity purified polyclonal antibody (A14936-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXL18 at approximately 100 kDa. The expected band size for FBXL18 is at 79 kDa. https://www.bosterbio.com/catalog/product/view/id/1341382026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08912-1-fbxo2-primary-antibodies-wb-testing-1.jpgAnti-FBXO2 Antibody Picoband®Western blot analysis of FBXO2 using anti-FBXO2 antibody (A08912-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXO2 antigen affinity purified polyclonal antibody (A08912-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXO2 at approximately 40 kDa. The expected band size for FBXO2 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08912-1-fbxo2-primary-antibodies-ihc-testing-1.jpgAnti-FBXO2 Antibody Picoband®IHC analysis of FBXO2 using anti-FBXO2 antibody (A08912-1). FBXO2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FBXO2 Antibody (A08912-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08912-1-fbxo2-primary-antibodies-ip-testing-1.jpgAnti-FBXO2 Antibody Picoband®Immunoprecipitating FBXO2 in HepG2 whole cell lysate. Western blot analysis of FBXO2 using anti-FBXO2 antibody (A08912-1). Lane 1: HepG2 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-FBXO2 antibody in HepG2 whole cell lysate, Lane 3: anti-FBXO2 antibody (2μg) + HepG2 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FBXO2 antigen affinity purified polyclonal antibody (A08912-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FBXO2 at approximately 40 kDa. The expected band size for FBXO2 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08912-1-fbxo2-primary-antibodies-fcm-testing-1.jpgAnti-FBXO2 Antibody Picoband®Flow Cytometry analysis of SiHa cells using anti-FBXO2 antibody (A08912-1). Overlay histogram showing SiHa cells stained with A08912-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FBXO2 Antibody (A08912-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341392026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15576-1-fbxo8-primary-antibodies-wb-testing-1.jpgAnti-FBXO8 Antibody Picoband®Western blot analysis of FBXO8 using anti-FBXO8 antibody (A15576-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXO8 antigen affinity purified polyclonal antibody (A15576-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXO8 at approximately 45 kDa. The expected band size for FBXO8 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15576-1-fbxo8-primary-antibodies-ihc-testing-1.jpgAnti-FBXO8 Antibody Picoband®IHC analysis of FBXO8 using anti-FBXO8 antibody (A15576-1). FBXO8 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FBXO8 Antibody (A15576-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15576-1-fbxo8-primary-antibodies-fcm-testing-1.jpgAnti-FBXO8 Antibody Picoband®Flow Cytometry analysis of U251 cells using anti-FBXO8 antibody (A15576-1). Overlay histogram showing U251 cells stained with A15576-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FBXO8 Antibody (A15576-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341402026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04708-1-fbxo11-primary-antibodies-wb-testing-1.jpgAnti-FBXO11 Antibody Picoband®Western blot analysis of FBXO11 using anti-FBXO11 antibody (A04708-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXO11 antigen affinity purified polyclonal antibody (A04708-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXO11 at approximately 120 kDa. The expected band size for FBXO11 is at 104 kDa. https://www.bosterbio.com/catalog/product/view/id/1341412026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09009-fbxo25-primary-antibodies-wb-testing-1.jpgAnti-FBXO25 Antibody Picoband®Western blot analysis of FBXO25 using anti-FBXO25 antibody (A09009). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXO25 antigen affinity purified polyclonal antibody (A09009) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXO25 at approximately 43 kDa. The expected band size for FBXO25 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09009-fbxo25-primary-antibodies-if-testing-1.jpgAnti-FBXO25 Antibody Picoband®IF analysis of FBXO25 using anti-FBXO25 antibody (A09009) and anti-Alpha Tubulin antibody (M03989-3). FBXO25 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FBXO25 Antibody (A09009) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1341422026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10940-1-fbxo28-primary-antibodies-wb-testing-1.jpgAnti-FBXO28 Antibody Picoband®Western blot analysis of FBXO28 using anti-FBXO28 antibody (A10940-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXO28 antigen affinity purified polyclonal antibody (A10940-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXO28 at approximately 41 kDa. The expected band size for FBXO28 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10940-1-fbxo28-primary-antibodies-ihc-testing-1.jpgAnti-FBXO28 Antibody Picoband®IHC analysis of FBXO28 using anti-FBXO28 antibody (A10940-1). FBXO28 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FBXO28 Antibody (A10940-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10940-1-fbxo28-primary-antibodies-ihc-testing-2.jpgAnti-FBXO28 Antibody Picoband®IHC analysis of FBXO28 using anti-FBXO28 antibody (A10940-1). FBXO28 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FBXO28 Antibody (A10940-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10940-1-fbxo28-primary-antibodies-ihc-testing-3.jpgAnti-FBXO28 Antibody Picoband®IHC analysis of FBXO28 using anti-FBXO28 antibody (A10940-1). FBXO28 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FBXO28 Antibody (A10940-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10940-1-fbxo28-primary-antibodies-if-testing-1.jpgAnti-FBXO28 Antibody Picoband®IF analysis of FBXO28 using anti-FBXO28 antibody (A10940-1) and anti-Alpha Tubulin antibody (M03989-3). FBXO28 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FBXO28 Antibody (A10940-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1341432026-04-03T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02609-3-tp53bp2-primary-antibodies-wb-testing-1.jpgAnti-53BP2/TP53BP2 Antibody Picoband®Western blot analysis of 53BP2/TP53BP2 using anti-53BP2/TP53BP2 antibody (A02609-3). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-53BP2/TP53BP2 antigen affinity purified polyclonal antibody (A02609-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for 53BP2/TP53BP2 at approximately 160 kDa. The expected band size for 53BP2/TP53BP2 is at 126 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02609-3-tp53bp2-primary-antibodies-ip-testing-1.jpgAnti-53BP2/TP53BP2 Antibody Picoband®Immunoprecipitating 53BP2/TP53BP2 in K562 whole cell lysate. Western blot analysis of 53BP2/TP53BP2 using anti-53BP2/TP53BP2 antibody (A02609-3). Lane 1: K562 whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-53BP2/TP53BP2 antibody in K562 whole cell lysate, Lane 3: anti-53BP2/TP53BP2 antibody (2μg) + K562 whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-53BP2/TP53BP2 antigen affinity purified polyclonal antibody (A02609-3) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 53BP2/TP53BP2 at approximately 160 kDa. The expected band size for 53BP2/TP53BP2 is at 126 kDa. https://www.bosterbio.com/catalog/product/view/id/1341442026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02815-2-eps15l1-primary-antibodies-wb-testing-1.jpgAnti-EPS15R/EPS15L1 Antibody Picoband®Western blot analysis of EPS15R/EPS15L1 using anti-EPS15R/EPS15L1 antibody (A02815-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPS15R/EPS15L1 antigen affinity purified polyclonal antibody (A02815-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPS15R/EPS15L1 at approximately 94 kDa. The expected band size for EPS15R/EPS15L1 is at 94 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02815-2-eps15l1-primary-antibodies-ihc-testing-1.jpgAnti-EPS15R/EPS15L1 Antibody Picoband®IHC analysis of EPS15R/EPS15L1 using anti-EPS15R/EPS15L1 antibody (A02815-2). EPS15R/EPS15L1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPS15R/EPS15L1 Antibody (A02815-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02815-2-eps15l1-primary-antibodies-ihc-testing-2.jpgAnti-EPS15R/EPS15L1 Antibody Picoband®IHC analysis of EPS15R/EPS15L1 using anti-EPS15R/EPS15L1 antibody (A02815-2). EPS15R/EPS15L1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPS15R/EPS15L1 Antibody (A02815-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02815-2-eps15l1-primary-antibodies-ihc-testing-3.jpgAnti-EPS15R/EPS15L1 Antibody Picoband®IHC analysis of EPS15R/EPS15L1 using anti-EPS15R/EPS15L1 antibody (A02815-2). EPS15R/EPS15L1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPS15R/EPS15L1 Antibody (A02815-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02815-2-eps15l1-primary-antibodies-ihc-testing-4.jpgAnti-EPS15R/EPS15L1 Antibody Picoband®IHC analysis of EPS15R/EPS15L1 using anti-EPS15R/EPS15L1 antibody (A02815-2). EPS15R/EPS15L1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPS15R/EPS15L1 Antibody (A02815-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02815-2-eps15l1-primary-antibodies-ihc-testing-5.jpgAnti-EPS15R/EPS15L1 Antibody Picoband®IHC analysis of EPS15R/EPS15L1 using anti-EPS15R/EPS15L1 antibody (A02815-2). EPS15R/EPS15L1 was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPS15R/EPS15L1 Antibody (A02815-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1341452026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04930-e4f1-primary-antibodies-wb-testing-1.jpgAnti-E4F1 Antibody Picoband®Western blot analysis of E4F1 using anti-E4F1 antibody (A04930). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E4F1 antigen affinity purified polyclonal antibody (A04930) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for E4F1 at approximately 120 kDa. The expected band size for E4F1 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04930-e4f1-primary-antibodies-if-testing-1.jpgAnti-E4F1 Antibody Picoband®IF analysis of E4F1 using anti-E4F1 antibody (A04930) and anti-Alpha Tubulin antibody (M03989-3). E4F1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-E4F1 Antibody (A04930) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04930-e4f1-primary-antibodies-fcm-testing-1.jpgAnti-E4F1 Antibody Picoband®Flow Cytometry analysis of Jurkat cells using anti-E4F1 antibody (A04930). Overlay histogram showing Jurkat cells stained with A04930 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-E4F1 Antibody (A04930, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341462026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13273-1-fbxo40-primary-antibodies-wb-testing-1.jpgAnti-FBXO40 Antibody Picoband®Western blot analysis of FBXO40 using anti-FBXO40 antibody (A13273-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates, Lane 2: monkey skeletal muscle tissue lysates, Lane 3: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXO40 antigen affinity purified polyclonal antibody (A13273-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXO40 at approximately 60 kDa. The expected band size for FBXO40 is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13273-1-fbxo40-primary-antibodies-ihc-testing-1.jpgAnti-FBXO40 Antibody Picoband®IHC analysis of FBXO40 using anti-FBXO40 antibody (A13273-1). FBXO40 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FBXO40 Antibody (A13273-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13273-1-fbxo40-primary-antibodies-ihc-testing-2.jpgAnti-FBXO40 Antibody Picoband®IHC analysis of FBXO40 using anti-FBXO40 antibody (A13273-1). FBXO40 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FBXO40 Antibody (A13273-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13273-1-fbxo40-primary-antibodies-ihc-testing-3.jpgAnti-FBXO40 Antibody Picoband®IHC analysis of FBXO40 using anti-FBXO40 antibody (A13273-1). FBXO40 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FBXO40 Antibody (A13273-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13273-1-fbxo40-primary-antibodies-ihc-testing-4.jpgAnti-FBXO40 Antibody Picoband®IHC analysis of FBXO40 using anti-FBXO40 antibody (A13273-1). FBXO40 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FBXO40 Antibody (A13273-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13273-1-fbxo40-primary-antibodies-ihc-testing-5.jpgAnti-FBXO40 Antibody Picoband®IHC analysis of FBXO40 using anti-FBXO40 antibody (A13273-1). FBXO40 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FBXO40 Antibody (A13273-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13273-1-fbxo40-primary-antibodies-fcm-testing-1.jpgAnti-FBXO40 Antibody Picoband®Flow Cytometry analysis of Hela cells using anti-FBXO40 antibody (A13273-1). Overlay histogram showing Hela cells stained with A13273-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FBXO40 Antibody (A13273-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341472026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06420-fcer1g-primary-antibodies-wb-testing-1.jpgAnti-FCER1G Antibody Picoband®Western blot analysis of FCER1G using anti-FCER1G antibody (A06420). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCER1G antigen affinity purified polyclonal antibody (A06420) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FCER1G at approximately 12 kDa. The expected band size for FCER1G is at 10 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06420-fcer1g-primary-antibodies-ihc-testing-1.jpgAnti-FCER1G Antibody Picoband®IHC analysis of FCER1G using anti-FCER1G antibody (A06420). FCER1G was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FCER1G Antibody (A06420) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06420-fcer1g-primary-antibodies-ihc-testing-2.jpgAnti-FCER1G Antibody Picoband®IHC analysis of FCER1G using anti-FCER1G antibody (A06420). FCER1G was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FCER1G Antibody (A06420) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06420-fcer1g-primary-antibodies-ihc-testing-3.jpgAnti-FCER1G Antibody Picoband®IHC analysis of FCER1G using anti-FCER1G antibody (A06420). FCER1G was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FCER1G Antibody (A06420) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06420-fcer1g-primary-antibodies-ihc-testing-4.jpgAnti-FCER1G Antibody Picoband®IHC analysis of FCER1G using anti-FCER1G antibody (A06420). FCER1G was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FCER1G Antibody (A06420) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06420-fcer1g-primary-antibodies-ip-testing-1.jpgAnti-FCER1G Antibody Picoband®Immunoprecipitating FCER1G in HEL whole cell lysate. Western blot analysis of FCER1G using anti-FCER1G antibody (A06420). Lane 1: HEL whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-FCER1G antibody in HEL whole cell lysate, Lane 3: anti-FCER1G antibody (2μg) + HEL whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FCER1G antigen affinity purified polyclonal antibody (A06420) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FCER1G at approximately 12 kDa. The expected band size for FCER1G is at 10 kDa. https://www.bosterbio.com/catalog/product/view/id/1341482026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16298-fcf1-primary-antibodies-wb-testing-1.jpgAnti-FCF1 Antibody Picoband®Western blot analysis of FCF1 using anti-FCF1 antibody (A16298). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCF1 antigen affinity purified polyclonal antibody (A16298) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FCF1 at approximately 23 kDa. The expected band size for FCF1 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16298-fcf1-primary-antibodies-fcm-testing-1.jpgAnti-FCF1 Antibody Picoband®Flow Cytometry analysis of SiHa cells using anti-FCF1 antibody (A16298). Overlay histogram showing SiHa cells stained with A16298 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FCF1 Antibody (A16298, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341492026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09023-1-fcho1-primary-antibodies-wb-testing-1.jpgAnti-FCHO1 Antibody Picoband®Western blot analysis of FCHO1 using anti-FCHO1 antibody (A09023-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCHO1 antigen affinity purified polyclonal antibody (A09023-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FCHO1 at approximately 110 kDa. The expected band size for FCHO1 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09023-1-fcho1-primary-antibodies-ihc-testing-1.jpgAnti-FCHO1 Antibody Picoband®IHC analysis of FCHO1 using anti-FCHO1 antibody (A09023-1). FCHO1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FCHO1 Antibody (A09023-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09023-1-fcho1-primary-antibodies-ihc-testing-2.jpgAnti-FCHO1 Antibody Picoband®IHC analysis of FCHO1 using anti-FCHO1 antibody (A09023-1). FCHO1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FCHO1 Antibody (A09023-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1341502026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07447-1-lrrc4-primary-antibodies-wb-testing-1.jpgAnti-LRRC4 Antibody Picoband®Western blot analysis of LRRC4 using anti-LRRC4 antibody (A07447-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human REH whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRC4 antigen affinity purified polyclonal antibody (A07447-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LRRC4 at approximately 80 kDa. The expected band size for LRRC4 is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07447-1-lrrc4-primary-antibodies-fcm-testing-1.jpgAnti-LRRC4 Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-LRRC4 antibody (A07447-1). Overlay histogram showing SH-SY5Y cells stained with A07447-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LRRC4 Antibody (A07447-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341512026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10034-fbxo30-primary-antibodies-wb-testing-1.jpgAnti-FBXO30 Antibody Picoband®Western blot analysis of FBXO30 using anti-FBXO30 antibody (A10034). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse NIH/3T3 tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBXO30 antigen affinity purified polyclonal antibody (A10034) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FBXO30 at approximately 82 kDa. The expected band size for FBXO30 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10034-fbxo30-primary-antibodies-fcm-testing-1.jpgAnti-FBXO30 Antibody Picoband®Flow Cytometry analysis of Hela cells using anti-FBXO30 antibody (A10034). Overlay histogram showing Hela cells stained with A10034 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FBXO30 Antibody (A10034, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341522026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13193-2-atp6v1c2-primary-antibodies-wb-testing-1.jpgAnti-ATP6V1C2 Antibody Picoband®Western blot analysis of ATP6V1C2 using anti-ATP6V1C2 antibody (A13193-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP6V1C2 antigen affinity purified polyclonal antibody (A13193-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATP6V1C2 at approximately 49 kDa. The expected band size for ATP6V1C2 is at 49 kDa. https://www.bosterbio.com/catalog/product/view/id/1341532026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10685-2-mdga2-primary-antibodies-wb-testing-1.jpgAnti-MDGA2 Antibody Picoband®Western blot analysis of MDGA2 using anti-MDGA2 antibody (A10685-2). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDGA2 antigen affinity purified polyclonal antibody (A10685-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MDGA2 at approximately 130 kDa. The expected band size for MDGA2 is at 107 kDa. https://www.bosterbio.com/catalog/product/view/id/1341542026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04210-1-myt1-primary-antibodies-wb-testing-1.jpgAnti-MYT1 Antibody Picoband®Western blot analysis of MYT1 using anti-MYT1 antibody (A04210-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human REH whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYT1 antigen affinity purified polyclonal antibody (A04210-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYT1 at approximately 122 kDa. The expected band size for MYT1 is at 122 kDa. https://www.bosterbio.com/catalog/product/view/id/1341552026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00753-4-glp1r-primary-antibodies-wb-testing-1.jpgAnti-GLP1R Antibody Picoband®Western blot analysis of GLP1R using anti-GLP1R antibody (A00753-4). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat small intestine tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLP1R antigen affinity purified polyclonal antibody (A00753-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GLP1R at approximately 53 kDa. The expected band size for GLP1R is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00753-4-glp1r-primary-antibodies-fcm-testing-1.jpgAnti-GLP1R Antibody Picoband®Flow Cytometry analysis of SH-SY5Y cells using anti-GLP1R antibody (A00753-4). Overlay histogram showing SH-SY5Y cells stained with A00753-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GLP1R Antibody (A00753-4, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341562026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13711-1-klf14-primary-antibodies-wb-testing-1.jpgAnti-SP6/KLF14 Antibody Picoband®Western blot analysis of SP6/KLF14 using anti-SP6/KLF14 antibody (A13711-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP6/KLF14 antigen affinity purified polyclonal antibody (A13711-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SP6/KLF14 at approximately 33 kDa. The expected band size for SP6/KLF14 is at 33 kDa. https://www.bosterbio.com/catalog/product/view/id/1341572026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08410-3-steap2-primary-antibodies-wb-testing-1.jpgAnti-STEAP2 Antibody Picoband®Western blot analysis of STEAP2 using anti-STEAP2 antibody (A08410-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat kidney tissue lysates Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STEAP2 antigen affinity purified polyclonal antibody (A08410-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STEAP2 at approximately 52 kDa. The expected band size for STEAP2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08410-3-steap2-primary-antibodies-ihc-testing-1.jpgAnti-STEAP2 Antibody Picoband®IHC analysis of STEAP2 using anti-STEAP2 antibody (A08410-3). STEAP2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STEAP2 Antibody (A08410-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08410-3-steap2-primary-antibodies-if-testing-1.jpgAnti-STEAP2 Antibody Picoband®IF analysis of STEAP2 using anti-STEAP2 antibody (A08410-3). STEAP2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STEAP2 Antibody (A08410-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1341582026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06977-1-fkbp6-primary-antibodies-wb-testing-1.jpgAnti-FKBP6 Antibody Picoband®Western blot analysis of FKBP6 using anti-FKBP6 antibody (A06977-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP6 antigen affinity purified polyclonal antibody (A06977-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FKBP6 at approximately 37 kDa. The expected band size for FKBP6 is at 37 kDa. https://www.bosterbio.com/catalog/product/view/id/1341592026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08789-1-myl1-primary-antibodies-wb-testing-1.jpgAnti-MYL1 Antibody Picoband®Western blot analysis of MYL1 using anti-MYL1 antibody (A08789-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL1 antigen affinity purified polyclonal antibody (A08789-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYL1 at approximately 22 kDa. The expected band size for MYL1 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08789-1-myl1-primary-antibodies-ihc-testing-1.jpgAnti-MYL1 Antibody Picoband®IHC analysis of MYL1 using anti-MYL1 antibody (A08789-1). MYL1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYL1 Antibody (A08789-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08789-1-myl1-primary-antibodies-ihc-testing-2.jpgAnti-MYL1 Antibody Picoband®IHC analysis of MYL1 using anti-MYL1 antibody (A08789-1). MYL1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYL1 Antibody (A08789-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08789-1-myl1-primary-antibodies-ihc-testing-3.jpgAnti-MYL1 Antibody Picoband®IHC analysis of MYL1 using anti-MYL1 antibody (A08789-1). MYL1 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYL1 Antibody (A08789-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08789-1-myl1-primary-antibodies-ihc-testing-4.jpgAnti-MYL1 Antibody Picoband®IHC analysis of MYL1 using anti-MYL1 antibody (A08789-1). MYL1 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYL1 Antibody (A08789-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08789-1-myl1-primary-antibodies-if-testing-1.jpgAnti-MYL1 Antibody Picoband®IF analysis of MYL1 using anti-MYL1 antibody (A08789-1). MYL1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MYL1 Antibody (A08789-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1341602026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06550-1-cldn14-primary-antibodies-wb-testing-1.jpgAnti-CLDN14 Antibody Picoband®Western blot analysis of CLDN14 using anti-CLDN14 antibody (A06550-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLDN14 antigen affinity purified polyclonal antibody (A06550-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CLDN14 at approximately 24 kDa. The expected band size for CLDN14 is at 26 kDa. https://www.bosterbio.com/catalog/product/view/id/1341612026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09576-1-fpr3-primary-antibodies-wb-testing-1.jpgAnti-FPR3 Antibody Picoband®Western blot analysis of FPR3 using anti-FPR3 antibody (A09576-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FPR3 antigen affinity purified polyclonal antibody (A09576-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FPR3 at approximately 40 kDa. The expected band size for FPR3 is at 40 kDa. https://www.bosterbio.com/catalog/product/view/id/1341622026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02718-1-azgp1-primary-antibodies-wb-testing-1.jpgAnti-Zinc Alpha 2 Glycoprotein/AZGP1 Antibody Picoband®Western blot analysis of Zinc Alpha 2 Glycoprotein/AZGP1 using anti-Zinc Alpha 2 Glycoprotein/AZGP1 antibody (A02718-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HUH-7 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Zinc Alpha 2 Glycoprotein/AZGP1 antigen affinity purified polyclonal antibody (A02718-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Zinc Alpha 2 Glycoprotein/AZGP1 at approximately 41,55 kDa. The expected band size for Zinc Alpha 2 Glycoprotein/AZGP1 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02718-1-azgp1-primary-antibodies-fcm-testing-1.jpgAnti-Zinc Alpha 2 Glycoprotein/AZGP1 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-Zinc Alpha 2 Glycoprotein/AZGP1 antibody (A02718-1). Overlay histogram showing MCF-7 cells stained with A02718-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Zinc Alpha 2 Glycoprotein/AZGP1 Antibody (A02718-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341632026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03097-3-ntm-primary-antibodies-wb-testing-1.jpgAnti-NTM Antibody Picoband®Western blot analysis of NTM using anti-NTM antibody (A03097-3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTM antigen affinity purified polyclonal antibody (A03097-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NTM at approximately 52 kDa. The expected band size for NTM is at 38 kDa. https://www.bosterbio.com/catalog/product/view/id/1341642026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11979-1-ints1-primary-antibodies-wb-testing-1.jpgAnti-INTS1 Antibody Picoband®Western blot analysis of INTS1 using anti-INTS1 antibody (A11979-1). Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse thymus tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INTS1 antigen affinity purified polyclonal antibody (A11979-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for INTS1 at approximately 244 kDa. The expected band size for INTS1 is at 244 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11979-1-ints1-primary-antibodies-ihc-testing-1.jpgAnti-INTS1 Antibody Picoband®IHC analysis of INTS1 using anti-INTS1 antibody (A11979-1). INTS1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-INTS1 Antibody (A11979-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/catalog/product/view/id/1341652026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/3/a32417-1-ints11-primary-antibodies-wb-testing-1.jpgAnti-INTS11 Antibody Picoband®Western blot analysis of INTS11 using anti-INTS11 antibody (A32417-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-INTS11 antigen affinity purified polyclonal antibody (A32417-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for INTS11 at approximately 68 kDa. The expected band size for INTS11 is at 68 kDa. https://www.bosterbio.com/catalog/product/view/id/1341662026-04-03T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10945-2-foxj3-primary-antibodies-wb-testing-1.jpgAnti-FOXJ3 Antibody Picoband®Western blot analysis of FOXJ3 using anti-FOXJ3 antibody (A10945-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXJ3 antigen affinity purified polyclonal antibody (A10945-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FOXJ3 at approximately 70-72 kDa. The expected band size for FOXJ3 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10945-2-foxj3-primary-antibodies-ihc-testing-1.jpgAnti-FOXJ3 Antibody Picoband®IHC analysis of FOXJ3 using anti-FOXJ3 antibody (A10945-2). FOXJ3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXJ3 Antibody (A10945-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10945-2-foxj3-primary-antibodies-ihc-testing-2.jpgAnti-FOXJ3 Antibody Picoband®IHC analysis of FOXJ3 using anti-FOXJ3 antibody (A10945-2). FOXJ3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXJ3 Antibody (A10945-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10945-2-foxj3-primary-antibodies-ihc-testing-3.jpgAnti-FOXJ3 Antibody Picoband®IHC analysis of FOXJ3 using anti-FOXJ3 antibody (A10945-2). FOXJ3 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXJ3 Antibody (A10945-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10945-2-foxj3-primary-antibodies-if-testing-1.jpgAnti-FOXJ3 Antibody Picoband®IF analysis of FOXJ3 using anti-FOXJ3 antibody (A10945-2) and anti-Alpha Tubulin antibody (M03989-3). FOXJ3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FOXJ3 Antibody (A10945-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/catalog/product/view/id/1341672026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02836-1-fpgs-primary-antibodies-wb-testing-1.jpgAnti-FPGS Antibody Picoband®Western blot analysis of FPGS using anti-FPGS antibody (A02836-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human JAR whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FPGS antigen affinity purified polyclonal antibody (A02836-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FPGS at approximately 65 kDa. The expected band size for FPGS is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02836-1-fpgs-primary-antibodies-if-testing-1.jpgAnti-FPGS Antibody Picoband®IF analysis of FPGS using anti-FPGS antibody (A02836-1) and anti-Alpha Tubulin antibody (M03989-3). FPGS was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FPGS Antibody (A02836-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02836-1-fpgs-primary-antibodies-fcm-testing-1.jpgAnti-FPGS Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-FPGS antibody (A02836-1). Overlay histogram showing K562 cells stained with A02836-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FPGS Antibody (A02836-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341682026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a16721-fsip1-primary-antibodies-wb-testing-1.jpgAnti-FSIP1 Antibody Picoband®Western blot analysis of FSIP1 using anti-FSIP1 antibody (A16721). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse TM4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSIP1 antigen affinity purified polyclonal antibody (A16721) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FSIP1 at approximately 80 kDa. The expected band size for FSIP1 is at 66 kDa. https://www.bosterbio.com/catalog/product/view/id/1341692026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14838-2-fstl4-primary-antibodies-wb-testing-1.jpgAnti-FSTL4 Antibody Picoband®Western blot analysis of FSTL4 using anti-FSTL4 antibody (A14838-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSTL4 antigen affinity purified polyclonal antibody (A14838-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FSTL4 at approximately 93 kDa. The expected band size for FSTL4 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14838-2-fstl4-primary-antibodies-fcm-testing-1.jpgAnti-FSTL4 Antibody Picoband®Flow Cytometry analysis of RT4 cells using anti-FSTL4 antibody (A14838-2). Overlay histogram showing RT4 cells stained with A14838-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-FSTL4 Antibody (A14838-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341702026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08695-2-fubp3-primary-antibodies-wb-testing-1.jpgAnti-FUBP3 Antibody Picoband®Western blot analysis of FUBP3 using anti-FUBP3 antibody (A08695-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FUBP3 antigen affinity purified polyclonal antibody (A08695-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FUBP3 at approximately 62 kDa. The expected band size for FUBP3 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08695-2-fubp3-primary-antibodies-ihc-testing-1.jpgAnti-FUBP3 Antibody Picoband®IHC analysis of FUBP3 using anti-FUBP3 antibody (A08695-2). FUBP3 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FUBP3 Antibody (A08695-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08695-2-fubp3-primary-antibodies-ihc-testing-2.jpgAnti-FUBP3 Antibody Picoband®IHC analysis of FUBP3 using anti-FUBP3 antibody (A08695-2). FUBP3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FUBP3 Antibody (A08695-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08695-2-fubp3-primary-antibodies-ihc-testing-3.jpgAnti-FUBP3 Antibody Picoband®IHC analysis of FUBP3 using anti-FUBP3 antibody (A08695-2). FUBP3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FUBP3 Antibody (A08695-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08695-2-fubp3-primary-antibodies-if-testing-1.jpgAnti-FUBP3 Antibody Picoband®IF analysis of FUBP3 using anti-FUBP3 antibody (A08695-2) and anti-Alpha Tubulin antibody (M03989-3). FUBP3 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FUBP3 Antibody (A08695-2) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08695-2-fubp3-primary-antibodies-fcm-testing-1.jpgAnti-FUBP3 Antibody Picoband®Flow Cytometry analysis of MCF-7 cells using anti-FUBP3 antibody (A08695-2). Overlay histogram showing MCF-7 cells stained with A08695-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FUBP3 Antibody (A08695-2, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341712026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07597-2-galm-primary-antibodies-wb-testing-1.jpgAnti-GALM Antibody Picoband®Western blot analysis of GALM using anti-GALM antibody (A07597-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALM antigen affinity purified polyclonal antibody (A07597-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GALM at approximately 38 kDa. The expected band size for GALM is at 38 kDa. https://www.bosterbio.com/catalog/product/view/id/1341722026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12824-galnt8-primary-antibodies-wb-testing-1.jpgAnti-GALNT8 Antibody Picoband®Western blot analysis of GALNT8 using anti-GALNT8 antibody (A12824). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT8 antigen affinity purified polyclonal antibody (A12824) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GALNT8 at approximately 90 kDa. The expected band size for GALNT8 is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12824-galnt8-primary-antibodies-ihc-testing-1.jpgAnti-GALNT8 Antibody Picoband®IHC analysis of GALNT8 using anti-GALNT8 antibody (A12824). GALNT8 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GALNT8 Antibody (A12824) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12824-galnt8-primary-antibodies-ihc-testing-2.jpgAnti-GALNT8 Antibody Picoband®IHC analysis of GALNT8 using anti-GALNT8 antibody (A12824). GALNT8 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GALNT8 Antibody (A12824) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12824-galnt8-primary-antibodies-fcm-testing-1.jpgAnti-GALNT8 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-GALNT8 antibody (A12824). Overlay histogram showing K562 cells stained with A12824 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GALNT8 Antibody (A12824, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341732026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09609-1-galnt11-primary-antibodies-wb-testing-1.jpgAnti-GALNT11 Antibody Picoband®Western blot analysis of GALNT11 using anti-GALNT11 antibody (A09609-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT11 antigen affinity purified polyclonal antibody (A09609-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GALNT11 at approximately 95 kDa. The expected band size for GALNT11 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09609-1-galnt11-primary-antibodies-ihc-testing-1.jpgAnti-GALNT11 Antibody Picoband®IHC analysis of GALNT11 using anti-GALNT11 antibody (A09609-1). GALNT11 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GALNT11 Antibody (A09609-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09609-1-galnt11-primary-antibodies-if-testing-1.jpgAnti-GALNT11 Antibody Picoband®IF analysis of GALNT11 using anti-GALNT11 antibody (A09609-1). GALNT11 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GALNT11 Antibody (A09609-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09609-1-galnt11-primary-antibodies-fcm-testing-1.jpgAnti-GALNT11 Antibody Picoband®Flow Cytometry analysis of K562 cells using anti-GALNT11 antibody (A09609-1). Overlay histogram showing K562 cells stained with A09609-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GALNT11 Antibody (A09609-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/catalog/product/view/id/1341742026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10529-1-gas2l3-primary-antibodies-wb-testing-1.jpgAnti-GAS2L3 Antibody Picoband®Western blot analysis of GAS2L3 using anti-GAS2L3 antibody (A10529-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAS2L3 antigen affinity purified polyclonal antibody (A10529-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GAS2L3 at approximately 75 kDa. The expected band size for GAS2L3 is at 75 kDa. https://www.bosterbio.com/catalog/product/view/id/1341752026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08125-1-fkbp9-primary-antibodies-wb-testing-1.jpgAnti-FKBP9 Antibody Picoband®Western blot analysis of FKBP9 using anti-FKBP9 antibody (A08125-1). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP9 antigen affinity purified polyclonal antibody (A08125-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FKBP9 at approximately 63 kDa. The expected band size for FKBP9 is at 63 kDa. https://www.bosterbio.com/catalog/product/view/id/1341762026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09416-odam-primary-antibodies-ihc-testing-1.jpgAnti-ODAM AntibodyIHC analysis of ODAM using anti-ODAM antibody (A09416). ODAM was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ODAM Antibody (A09416) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09416-odam-primary-antibodies-ihc-testing-2.jpgAnti-ODAM AntibodyIHC analysis of ODAM using anti-ODAM antibody (A09416). ODAM was detected in a paraffin-embedded section of rat tooth tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ODAM Antibody (A09416) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/anti-alpha-smooth-muscle-actin-antibody-monoclonal-m01072-7-boster.html2026-04-03T05:00:57+00:00daily0.9